Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0002142

Physical Location & Seq

Genomic Coordinates Forward (+)

29323213 .. 29334271

Gene Structure 11059 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG268800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2748 bp

ATGGCAGAAGACGGCCTACGGTTAGGTGGGCCTTCTTCTGGTCTGTTCTTGATATTGAATGGTGAGGACAGCAAAGAGAATTCGTCGAAATCCCATCTTGTTTCATCATGTGATGAGCTCGGTTACCAATCAGTGGAGCGAACCTTGGAATATGTATTTGGACTCCCTAACAGATCAATCGGTTCGTTGTCTGGGCCAGTTGATGGGAATCTAGTTCACGGTATTTTAAAGAATGAGTTCTCGAAATTACATGCAAATTCAAGTACTTTGGATGGAGACAGAGACGGCATTTGCATTTCTGGGAATGGCTGCAGACCCCACACCATTGGTCTTGATGAAATCAGTATCCGGGGTGATATTGGAATCGTTAAGCCACCTTTGCTTGTAGAGAGCTTAGCAATGTTCAGCAGTGCTAGGGCTAACGCTTCTGTTTGGAAAGGAAAATGGATGTATGAAGTTGTTCTGGAAACTGCAGGTATTCAGCAGCTTGGATGGGCTACTGTTTCTTGCCCTTTCACTGACCATAAGGGTGTAGGTGATGCTGATGATTCATATGCGTTTGATGGGAGGAGGGTTAGAAAATGGAACAAGGATGCTGAGTCATATGGTCAGTCATGGGTTGTTGGTGATGCTATTGGATGCTGCATAGATTTGGACTGTAATGAAATATCATTCTACAGAAATGGTGTCTCTCTTGGTTTGGCATTCCATGGTATTCGTAAGATGGGACCAGGATGTGGATATTATCCTGCAGTTTCTCTCTCACAAGGTGAACGATGTGAATTAAATTTTGGTGGCCGTCCCTTTAAATTTCCAATTGAAGGCTACCAGCCCCTTCAAGCACCTCCTCCTTTAAATTTGTTTGCAACTCAGTTGCTGCAGTGCTTGTCAAGGTTGTTGGGTTTGCACTCTGTGGAGCAGGCAAAACATTCTTCTGTTGAGAAATTGAGACTGAAGAGGTTTGTTTCTCCTGACGAAATTTTTTATCCTGCTTCTCAGGGGATATGTGAGGAACTTTTATCTGTACTTGGAGCTGATGTTTGGAGCATAGAGTATGTTGCCTGGGGTCCGTTTTTGTCATTCATGGTGGAAGTGTTTGGATTGCAGGCGCCACATGATTATTCAGGCTTAGATAGAGTTCTGGATGTCTTTCTAGAATTTAATAGATCACACTTGTTGTTTGAACATATTATAAATGCCCTGGCATGTGGTTGCAAAACGGCCACATTGGTTCTCAAAGAGTGCCCATGGTCAGGGTCATATCCATACCTTGCATTGGCATGTCATATCCTGAGACGGCAGGAACTTATGGTGCTCTGGTGGAAATCACCTGATTTTGAATACCATTTTGAAAGGTTCCTATCACGAAAGGATCCAAATAAGAATGATCTGGAATCCATGATGCCTTCTGTTTGGTGGCCTGGTTCATGTGAGGATGTGTCCTACGAAAGTAGCATGCTGTTGACGACTACAGCTTTATCTGAAGCTGTTAGTAAGATTGAGGAGAAGCATAGGGACCTCTGTCGCTTGGTCATACAGTTCATACCACCTGTGACACCTCCCCAGTTGCCTGGTTCAGTGTTTAGGACATTTCTACAGAACCTTCTACTAAAGAACAGAGGAGCTGACCGCAATCTTCCACCTCCTGGAGTTTCAAGCAATTCTGTTCTTGTTTCTTTGTACACAGTTATACTTCATTTTTTATCTGAGGGGTTTGGAATGGGGGAAATATGCGGTTGGTTGAAGCACAGTGAAAAAGGTCATGATGTTGGTTTTCTTCATAGGGGTGGTCATCGAAGCTTTCCAGTAGGTTTATTTCTTAGAAATGATCCTCATCGAAATGACAATACCAGGCTTGGAGGATCTTTTAGTCATCTCTCAAAATCACACCCTGTAGATGATCAGGAAGCAGAAGATATCCGGTGGGAGGAAGGTTGTATGGATGATGAAGAAACGAGAGTGACTCATCTAAGTACAAGAAAACCATGCTGCTGCTCAAGTTACGACGAGGATTTTACAAGAATCTCAAAGAATCCAATTAGATACACAGCTAAAGGTTCCCGTGCCCATTGCAGTCCCATGCCAGAGAGATCTTCCCATGTTACTACAGAATGCAGTGCTGGAAGTTTGAGTGATGATATGGCTGATAAACCTAGTTCTAGCTACCAATCTGAATCCGATTTTAGTTATTGCCTGGTGCAACATTCGAGTTTTGTACCAAGGGAGGGTATTATGTCGTCAGCTACACTCAGAGAAGAAGAACTTCTAGATGTTCTATTGCTGTTGTATCATATAGGTCTTGCTCCAAATTTTAAGCAGGCATCGTATTACATGAATCACCAGTTGCAGTCAATTGCCAATCTTGAAGAAACTGATAAAAAAATTCGAGAAGGACCTTGTTTTGAGCAATTGAAGCATTTGAAAGAAGCTCGTAATGTTCATAGAGAAGAGGTTATTGATTCTGTAAGACAATGCGCTTGGTACCGTATCTCCCTATCCTCTAGATGGAAGCAAAGAGGAATGTATGCAACATGCATGTGGACTGTTCAGTTGCTTCTTGTCCTTAGCAAAGTGGATTTGTTGTTCACTTATGTCCCTGAATACTATCTTGAAGCTCTGGTAAATCAAATGTTTTGTTTTATTCAAACAACAGTCTTACATACGCAAGGAGTCCCTTTCATAAAATTGTTCAATCATTTTTTTTATATTATGAACACTTGTATTTGCCCTTGTTTTTGTCGCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030163 GO:0032446 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0060154 GO:0065007 GO:0070647 GO:0071704 GO:0098586 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPRY Ufd2P_core zf-C3HC4_3

Protein Analysis

915

Amino Acids

102.96

Weight (kDa)

5.9

Isoelectric Point (pI)

49.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RKP_N	PF19322	1 - 137	6.5e-59	E3 ubiquitin-protein ligase RKP N-terminus
SPRY	PF00622	148 - 266	6.9e-30	SPRY domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22010 AT2G22010
fragaria_vesca	FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_7g04730 FvH4_7g04730
malus_domestica	MD01G1017400.v1.1 MD15G1333600.v1.1
prunus_persica	Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1
pyrus_communis	pycom01g04820 pycom01g04830 pycom15g29160 pycom15g29170
rosa_chinensis	RchiOBHm_Chr2g0116231
rosa_laevigata	RLG00000018249
rosa_multiflora	Rmu_sc0003499.1_g000012 Rmu_sc0004888.1_g000064
rosa_roxburghii	Rroxscaffold_2G00127410
rosa_rugosa	Rorug02G0205000
rosa_samantha	Rh2AG260600 Rh2BG271700 Rh2CG266800 Rh2DG268800
rosa_wichuraiana	Rw2G020430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1193
Acc36I	ACCTGC	1 cut(s)	464
Acc65I	GGTACC	1 cut(s)	2481
AccB1I	GGYRCC	2 cut(s)	1108, 2481
AccB7I	CCANNNNNTGG	4 cut(s)	133, 203, 737, 1646
AciI	CCGC	2 cut(s)	1630, 1734
AclWI	GGATC	4 cut(s)	1367, 1380, 1823, 1870
AcoI	YGGCCR	2 cut(s)	796, 1221
AcsI	RAATTY	9 cut(s)	79, 256, 787, 809, 856, 978, 1157, 2308, 2382
AcuI	CTGAAG	2 cut(s)	974, 1503
AcyI	GRCGYC	1 cut(s)	1109
AdeI	CACNNNGTG	2 cut(s)	770, 913
AfaI	GTAC	6 cut(s)	265, 1026, 1682, 1975, 2217, 2483
AjnI	CCWGG	8 cut(s)	730, 1061, 1200, 1420, 1570, 1645, 1850, 2193
AjuI	GAANNNNNNNTTGG	4 cut(s)	141, 173, 774, 806
AloI	GAACNNNNNNTCC	2 cut(s)	2567, 2599
Alw21I	GWGCWC	2 cut(s)	120, 1317
Alw26I	GTCTC	5 cut(s)	270, 276, 694, 943, 1288
AlwI	GGATC	4 cut(s)	1367, 1380, 1823, 1870
AlwNI	CAGNNNCTG	1 cut(s)	877
AoxI	GGCC	6 cut(s)	13, 29, 194, 796, 1221, 1418
ApeKI	GCWGC	6 cut(s)	309, 484, 642, 877, 1989, 1992
ApoI	RAATTY	9 cut(s)	79, 256, 787, 809, 856, 978, 1157, 2308, 2382
Asp700I	GAANNNNTTC	3 cut(s)	236, 459, 2262
Asp718I	GGTACC	1 cut(s)	2481
AspLEI	GCGC	2 cut(s)	1111, 2477
AspS9I	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
AsuC2I	CCSGG	1 cut(s)	350
AsuHPI	GGTGA	7 cut(s)	74, 365, 548, 638, 782, 1320, 2330
AvaII	GGWCC	4 cut(s)	728, 1067, 1516, 2393
BaeGI	GKGCMC	2 cut(s)	1247, 2068
BamHI	GGATCC	1 cut(s)	1372
BanI	GGYRCC	2 cut(s)	1108, 2481
BanII	GRGCYC	1 cut(s)	120
BarI	GAAGNNNNNNTAC	1 cut(s)	32
BbsI	GAAGAC	1 cut(s)	15
Bbv12I	GWGCWC	2 cut(s)	120, 1317
BbvI	GCAGC	6 cut(s)	296, 496, 629, 864, 1976, 1979
BccI	CCATC	7 cut(s)	102, 197, 266, 486, 557, 718, 2499
BceAI	ACGGC	5 cut(s)	28, 301, 783, 1236, 1313
BciT130I	CCWGG	8 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2195
BciVI	GTATCC	1 cut(s)	356
BclI	TGATCA	1 cut(s)	1900
BcnI	CCSGG	1 cut(s)	350
BcoDI	GTCTC	5 cut(s)	270, 276, 694, 943, 1288
BfaI	CTAG	7 cut(s)	212, 414, 1154, 2154, 2160, 2267, 2502
BfmI	CTRYAG	9 cut(s)	310, 471, 676, 750, 878, 1470, 1595, 1893, 2106
BfoI	RGCGCY	1 cut(s)	1112
BfuAI	ACCTGC	1 cut(s)	464
BfuI	GTATCC	1 cut(s)	356
BglII	AGATCT	1 cut(s)	2090
BisI	GCNGC	6 cut(s)	310, 485, 643, 878, 1990, 1993
BlpI	GCTNAGC	1 cut(s)	394
BlsI	GCNGC	6 cut(s)	311, 486, 644, 879, 1991, 1994
BmcAI	AGTACT	1 cut(s)	265
Bme1390I	CCNGG	9 cut(s)	350, 732, 1063, 1202, 1422, 1572, 1647, 1852, 2195
Bme18I	GGWCC	4 cut(s)	728, 1067, 1516, 2393
BmgT120I	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
BmiI	GGNNCC	8 cut(s)	729, 1068, 1110, 1358, 1374, 1517, 2059, 2483
BmrFI	CCNGG	9 cut(s)	350, 732, 1063, 1202, 1422, 1572, 1647, 1852, 2195
BmrI	ACTGGG	1 cut(s)	1558
BmsI	GCATC	6 cut(s)	529, 583, 619, 629, 1392, 2330
BmuI	ACTGGG	1 cut(s)	1558
BoxI	GACNNNNGTC	1 cut(s)	1521
BpiI	GAAGAC	1 cut(s)	15
BplI	GAGNNNNNCTC	2 cut(s)	1948, 1980
BpmI	CTGGAG	1 cut(s)	1668
Bpu10I	CCTNAGC	1 cut(s)	2564
Bpu1102I	GCTNAGC	1 cut(s)	394
BpuEI	CTTGAG	1 cut(s)	1981
BpuMI	CCSGG	1 cut(s)	350
BsaBI	GATNNNNATC	2 cut(s)	207, 1833
BsaHI	GRCGYC	1 cut(s)	1109
BsaJI	CCNNGG	7 cut(s)	144, 349, 709, 1062, 1200, 1247, 2219
BsaWI	WCCGGW	1 cut(s)	1920
BsaXI	ACNNNNNCTCC	4 cut(s)	1023, 1053, 2474, 2504
Bse1I	ACTGG	4 cut(s)	197, 1564, 1805, 2341
Bse3DI	GCAATG	2 cut(s)	405, 2068
Bse8I	GATNNNNATC	2 cut(s)	207, 1833
BseBI	CCWGG	8 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2195
BseDI	CCNNGG	7 cut(s)	144, 349, 709, 1062, 1200, 1247, 2219
BseGI	GGATG	9 cut(s)	277, 453, 497, 598, 644, 740, 1150, 1441, 1948
BseJI	GATNNNNATC	2 cut(s)	207, 1833
BseMI	GCAATG	2 cut(s)	405, 2068
BseMII	CTCAG	6 cut(s)	588, 884, 1010, 1283, 1698, 2263
BseNI	ACTGG	4 cut(s)	197, 1564, 1805, 2341
BseRI	GAGGAG	4 cut(s)	583, 837, 1517, 1635
BseSI	GKGCMC	2 cut(s)	1247, 2068
BseXI	GCAGC	6 cut(s)	296, 496, 629, 864, 1976, 1979
BshFI	GGCC	6 cut(s)	15, 31, 196, 798, 1223, 1420
BshNI	GGYRCC	2 cut(s)	1108, 2481
BsiHKAI	GWGCWC	2 cut(s)	120, 1317
BsiSI	CCGG	2 cut(s)	349, 1921
BslFI	GGGAC	6 cut(s)	741, 786, 1529, 2061, 2579, 2657
BsmAI	GTCTC	5 cut(s)	270, 276, 694, 943, 1288
BsmBI	CGTCTC	2 cut(s)	276, 1288
BsmFI	GGGAC	6 cut(s)	741, 786, 1529, 2061, 2579, 2657
BsmI	GAATGC	2 cut(s)	704, 2117
BsnI	GGCC	6 cut(s)	15, 31, 196, 798, 1223, 1420
Bsp1286I	GDGCHC	4 cut(s)	120, 1247, 1317, 2068
Bsp1407I	TGTACA	1 cut(s)	1680
Bsp143I	GATC	8 cut(s)	173, 1166, 1372, 1387, 1828, 1862, 1900, 2090
Bsp1720I	GCTNAGC	1 cut(s)	394
Bsp19I	CCATGG	2 cut(s)	709, 1247
BspACI	CCGC	2 cut(s)	1630, 1734
BspANI	GGCC	6 cut(s)	15, 31, 196, 798, 1223, 1420
BspCNI	CTCAG	6 cut(s)	589, 883, 1009, 1284, 1699, 2262
BspHI	TCATGA	1 cut(s)	1762
BspLI	GGNNCC	8 cut(s)	729, 1068, 1110, 1358, 1374, 1517, 2059, 2483
BspMAI	CTGCAG	4 cut(s)	314, 475, 754, 882
BspMI	ACCTGC	1 cut(s)	464
BspPI	GGATC	4 cut(s)	1367, 1380, 1823, 1870
BspT107I	GGYRCC	2 cut(s)	1108, 2481
BsrDI	GCAATG	2 cut(s)	405, 2068
BsrGI	TGTACA	1 cut(s)	1680
BsrI	ACTGG	4 cut(s)	197, 1564, 1805, 2341
BssECI	CCNNGG	7 cut(s)	144, 349, 709, 1062, 1200, 1247, 2219
BssMI	GATC	8 cut(s)	173, 1166, 1372, 1387, 1828, 1862, 1900, 2090
BssNI	GRCGYC	1 cut(s)	1109
BssT1I	CCWWGG	4 cut(s)	144, 709, 1247, 2219
Bst2UI	CCWGG	8 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2195
Bst6I	CTCTTC	2 cut(s)	950, 2442
BstACI	GRCGYC	1 cut(s)	1109
BstAUI	TGTACA	1 cut(s)	1680
BstC8I	GCNNGC	4 cut(s)	921, 1107, 1457, 2319
BstDSI	CCRYGG	2 cut(s)	709, 1247
BstEII	GGTNACC	1 cut(s)	122
BstF5I	GGATG	9 cut(s)	277, 453, 497, 598, 644, 740, 1150, 1441, 1948
BstH2I	RGCGCY	1 cut(s)	1112
BstHHI	GCGC	2 cut(s)	1111, 2477
BstKTI	GATC	8 cut(s)	176, 1169, 1375, 1390, 1831, 1865, 1903, 2093
BstMAI	GTCTC	5 cut(s)	270, 276, 694, 943, 1288
BstMBI	GATC	8 cut(s)	173, 1166, 1372, 1387, 1828, 1862, 1900, 2090
BstMWI	GCNNNNNNNGC	2 cut(s)	379, 2413
BstNI	CCWGG	8 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2195
BstNSI	RCATGY	7 cut(s)	254, 1209, 1284, 1459, 2535, 2539, 2746
BstPAI	GACNNNNGTC	1 cut(s)	1521
BstPI	GGTNACC	1 cut(s)	122
BstSCI	CCNGG	9 cut(s)	348, 730, 1061, 1200, 1420, 1570, 1645, 1850, 2193
BstSFI	CTRYAG	9 cut(s)	310, 471, 676, 750, 878, 1470, 1595, 1893, 2106
BstSLI	GKGCMC	2 cut(s)	1247, 2068
BstV1I	GCAGC	6 cut(s)	296, 496, 629, 864, 1976, 1979
BstV2I	GAAGAC	1 cut(s)	15
BstX2I	RGATCY	3 cut(s)	1372, 1862, 2090
BstXI	CCANNNNNNTGG	2 cut(s)	326, 1571
BstYI	RGATCY	3 cut(s)	1372, 1862, 2090
BsuI	GTATCC	1 cut(s)	356
BsuRI	GGCC	6 cut(s)	15, 31, 196, 798, 1223, 1420
BtgI	CCRYGG	2 cut(s)	709, 1247
BtsCI	GGATG	9 cut(s)	277, 453, 497, 598, 644, 740, 1150, 1441, 1948
BtsI	GCAGTG	3 cut(s)	415, 887, 2122
BtsIMutI	CAGTG	7 cut(s)	138, 415, 516, 887, 1584, 1756, 2122
BveI	ACCTGC	1 cut(s)	464
Cac8I	GCNNGC	4 cut(s)	921, 1107, 1457, 2319
CaiI	CAGNNNCTG	1 cut(s)	877
CciI	TCATGA	1 cut(s)	1762
CfoI	GCGC	2 cut(s)	1111, 2477
Cfr13I	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
Csp6I	GTAC	6 cut(s)	264, 1025, 1681, 1974, 2216, 2482
CviQI	GTAC	6 cut(s)	264, 1025, 1681, 1974, 2216, 2482
DinI	GGCGCC	1 cut(s)	1110
DpnI	GATC	8 cut(s)	175, 1168, 1374, 1389, 1830, 1864, 1902, 2092
DpnII	GATC	8 cut(s)	173, 1166, 1372, 1387, 1828, 1862, 1900, 2090
DraI	TTTAAA	3 cut(s)	228, 808, 855
DraIII	CACNNNGTG	2 cut(s)	770, 913
EaeI	YGGCCR	2 cut(s)	796, 1221
Eam1104I	CTCTTC	2 cut(s)	950, 2442
EarI	CTCTTC	2 cut(s)	950, 2442
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	4 cut(s)	144, 709, 1247, 2219
Eco24I	GRGCYC	1 cut(s)	120
Eco32I	GATATC	1 cut(s)	1918
Eco47I	GGWCC	4 cut(s)	728, 1067, 1516, 2393
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	2 cut(s)	974, 1503
Eco91I	GGTNACC	1 cut(s)	122
EcoICRI	GAGCTC	1 cut(s)	118
EcoO109I	RGGNCCY	2 cut(s)	1516, 2393
EcoO65I	GGTNACC	1 cut(s)	122
EcoRI	GAATTC	1 cut(s)	79
EcoRII	CCWGG	8 cut(s)	730, 1061, 1200, 1420, 1570, 1645, 1850, 2193
EcoRV	GATATC	1 cut(s)	1918
EcoT14I	CCWWGG	4 cut(s)	144, 709, 1247, 2219
EcoT22I	ATGCAT	1 cut(s)	2537
EcoT38I	GRGCYC	1 cut(s)	120
EgeI	GGCGCC	1 cut(s)	1110
EheI	GGCGCC	1 cut(s)	1110
ErhI	CCWWGG	4 cut(s)	144, 709, 1247, 2219
Esp3I	CGTCTC	2 cut(s)	276, 1288
FalI	AAGNNNNNCTT	2 cut(s)	2247, 2279
FaqI	GGGAC	6 cut(s)	741, 786, 1529, 2061, 2579, 2657
FauNDI	CATATG	2 cut(s)	553, 604
FbaI	TGATCA	1 cut(s)	1900
Fnu4HI	GCNGC	6 cut(s)	310, 485, 643, 878, 1990, 1993
FokI	GGATG	9 cut(s)	284, 460, 504, 605, 651, 747, 1157, 1448, 1955
FriOI	GRGCYC	1 cut(s)	120
Fsp4HI	GCNGC	6 cut(s)	310, 485, 643, 878, 1990, 1993
FspBI	CTAG	7 cut(s)	212, 414, 1154, 2154, 2160, 2267, 2502
GlaI	GCGC	2 cut(s)	1110, 2476
GluI	GCNGC	6 cut(s)	310, 485, 643, 878, 1990, 1993
GsuI	CTGGAG	1 cut(s)	1668
HaeII	RGCGCY	1 cut(s)	1112
HaeIII	GGCC	6 cut(s)	15, 31, 196, 798, 1223, 1420
HapII	CCGG	2 cut(s)	349, 1921
HhaI	GCGC	2 cut(s)	1111, 2477
Hin1I	GRCGYC	1 cut(s)	1109
Hin6I	GCGC	2 cut(s)	1109, 2475
HinP1I	GCGC	2 cut(s)	1109, 2475
HincII	GTYRAC	1 cut(s)	1464
HindII	GTYRAC	1 cut(s)	1464
HindIII	AAGCTT	1 cut(s)	1798
HpaII	CCGG	2 cut(s)	349, 1921
HphI	GGTGA	7 cut(s)	74, 365, 548, 638, 782, 1320, 2330
Hpy166II	GTNNAC	6 cut(s)	217, 773, 1464, 1683, 2541, 2586
Hpy188I	TCNGA	5 cut(s)	1483, 1708, 2173, 2179, 2252
Hpy8I	GTNNAC	6 cut(s)	217, 773, 1464, 1683, 2541, 2586
Hpy99I	CGWCG	2 cut(s)	88, 2009
HpyAV	CCTTC	7 cut(s)	42, 815, 845, 1416, 1613, 1925, 2384
HpyF10VI	GCNNNNNNNGC	2 cut(s)	379, 2413
Hsp92I	GRCGYC	1 cut(s)	1109
HspAI	GCGC	2 cut(s)	1109, 2475
KasI	GGCGCC	1 cut(s)	1108
KpnI	GGTACC	1 cut(s)	2485
Ksp22I	TGATCA	1 cut(s)	1900
Kzo9I	GATC	8 cut(s)	173, 1166, 1372, 1387, 1828, 1862, 1900, 2090
LmnI	GCTCC	6 cut(s)	136, 916, 1031, 1044, 1622, 2308
Lsp1109I	GCAGC	6 cut(s)	296, 496, 629, 864, 1976, 1979
LweI	GCATC	6 cut(s)	529, 583, 619, 629, 1392, 2330
MaeI	CTAG	7 cut(s)	212, 414, 1154, 2154, 2160, 2267, 2502
MaeIII	GTNAC	5 cut(s)	122, 1552, 1960, 2000, 2101
MalI	GATC	8 cut(s)	175, 1168, 1374, 1389, 1830, 1864, 1902, 2092
MboI	GATC	8 cut(s)	173, 1166, 1372, 1387, 1828, 1862, 1900, 2090
MfeI	CAATTG	3 cut(s)	816, 2352, 2408
MflI	RGATCY	3 cut(s)	1372, 1862, 2090
MhlI	GDGCHC	4 cut(s)	120, 1247, 1317, 2068
Mly113I	GGCGCC	1 cut(s)	1109
MlyI	GAGTC	4 cut(s)	156, 608, 1957, 2679
Mph1103I	ATGCAT	1 cut(s)	2537
MroXI	GAANNNNTTC	3 cut(s)	236, 459, 2262
MseI	TTAA	7 cut(s)	227, 369, 785, 807, 854, 1161, 2313
MslI	CAYNNNNRTG	5 cut(s)	528, 1279, 1785, 1839, 2536
MspI	CCGG	2 cut(s)	349, 1921
MspR9I	CCNGG	9 cut(s)	350, 732, 1063, 1202, 1422, 1572, 1647, 1852, 2195
MunI	CAATTG	3 cut(s)	816, 2352, 2408
Mva1269I	GAATGC	2 cut(s)	704, 2117
MvaI	CCWGG	8 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2195
MwoI	GCNNNNNNNGC	2 cut(s)	379, 2413
NarI	GGCGCC	1 cut(s)	1109
NciI	CCSGG	1 cut(s)	350
NcoI	CCATGG	2 cut(s)	709, 1247
NdeI	CATATG	2 cut(s)	553, 604
NdeII	GATC	8 cut(s)	173, 1166, 1372, 1387, 1828, 1862, 1900, 2090
NlaIV	GGNNCC	8 cut(s)	729, 1068, 1110, 1358, 1374, 1517, 2059, 2483
NmuCI	GTSAC	2 cut(s)	1552, 1960
NsiI	ATGCAT	1 cut(s)	2537
NspI	RCATGY	7 cut(s)	254, 1209, 1284, 1459, 2535, 2539, 2746
PaeI	GCATGC	1 cut(s)	1459
PagI	TCATGA	1 cut(s)	1762
PctI	GAATGC	2 cut(s)	704, 2117
PdmI	GAANNNNTTC	3 cut(s)	236, 459, 2262
PfeI	GAWTC	9 cut(s)	208, 363, 548, 1394, 2022, 2032, 2174, 2335, 2459
PflMI	CCANNNNNTGG	4 cut(s)	133, 203, 737, 1646
PfoI	TCCNGGA	1 cut(s)	1645
PkrI	GCNGC	6 cut(s)	311, 486, 644, 879, 1991, 1994
PleI	GAGTC	4 cut(s)	156, 607, 1957, 2678
PluTI	GGCGCC	1 cut(s)	1112
PpsI	GAGTC	4 cut(s)	156, 607, 1957, 2678
PpuMI	RGGWCCY	2 cut(s)	1516, 2393
PshAI	GACNNNNGTC	1 cut(s)	1521
PsiI	TTATAA	1 cut(s)	1193
Psp124BI	GAGCTC	1 cut(s)	120
Psp5II	RGGWCCY	2 cut(s)	1516, 2393
Psp6I	CCWGG	8 cut(s)	730, 1061, 1200, 1420, 1570, 1645, 1850, 2193
PspEI	GGTNACC	1 cut(s)	122
PspGI	CCWGG	8 cut(s)	730, 1061, 1200, 1420, 1570, 1645, 1850, 2193
PspN4I	GGNNCC	8 cut(s)	729, 1068, 1110, 1358, 1374, 1517, 2059, 2483
PspPI	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
PspPPI	RGGWCCY	2 cut(s)	1516, 2393
PstI	CTGCAG	4 cut(s)	314, 475, 754, 882
PstNI	CAGNNNCTG	1 cut(s)	877
PsuI	RGATCY	3 cut(s)	1372, 1862, 2090
RsaI	GTAC	6 cut(s)	265, 1026, 1682, 1975, 2217, 2483
RsaNI	GTAC	6 cut(s)	264, 1025, 1681, 1974, 2216, 2482
RseI	CAYNNNNRTG	5 cut(s)	528, 1279, 1785, 1839, 2536
SacI	GAGCTC	1 cut(s)	120
SaqAI	TTAA	7 cut(s)	227, 369, 785, 807, 854, 1161, 2313
SatI	GCNGC	6 cut(s)	310, 485, 643, 878, 1990, 1993
Sau3AI	GATC	8 cut(s)	173, 1166, 1372, 1387, 1828, 1862, 1900, 2090
Sau96I	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
ScaI	AGTACT	1 cut(s)	265
SchI	GAGTC	4 cut(s)	156, 608, 1957, 2679
ScrFI	CCNGG	9 cut(s)	350, 732, 1063, 1202, 1422, 1572, 1647, 1852, 2195
SduI	GDGCHC	4 cut(s)	120, 1247, 1317, 2068
SfaNI	GCATC	6 cut(s)	529, 583, 619, 629, 1392, 2330
SfcI	CTRYAG	9 cut(s)	310, 471, 676, 750, 878, 1470, 1595, 1893, 2106
SfoI	GGCGCC	1 cut(s)	1110
SinI	GGWCC	4 cut(s)	728, 1067, 1516, 2393
SmiMI	CAYNNNNRTG	5 cut(s)	528, 1279, 1785, 1839, 2536
SmlI	CTYRAG	1 cut(s)	1996
SmoI	CTYRAG	1 cut(s)	1996
SphI	GCATGC	1 cut(s)	1459
SsiI	CCGC	2 cut(s)	1630, 1734
SspDI	GGCGCC	1 cut(s)	1108
SspMI	CTAG	7 cut(s)	212, 414, 1154, 2154, 2160, 2267, 2502
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	9 cut(s)	348, 730, 1061, 1200, 1420, 1570, 1645, 1850, 2193
StyI	CCWWGG	4 cut(s)	144, 709, 1247, 2219
TaqI	TCGA	6 cut(s)	86, 242, 1795, 1837, 2207, 2386
TatI	WGTACW	4 cut(s)	263, 1024, 1680, 1973
TfiI	GAWTC	9 cut(s)	208, 363, 548, 1394, 2022, 2032, 2174, 2335, 2459
Tru1I	TTAA	7 cut(s)	227, 369, 785, 807, 854, 1161, 2313
Tru9I	TTAA	7 cut(s)	227, 369, 785, 807, 854, 1161, 2313
TscAI	CASTG	7 cut(s)	138, 415, 523, 887, 1584, 1756, 2122
TseFI	GTSAC	2 cut(s)	1552, 1960
TseI	GCWGC	6 cut(s)	309, 484, 642, 877, 1989, 1992
Tsp45I	GTSAC	2 cut(s)	1552, 1960
TspGWI	ACGGA	1 cut(s)	1059
TspRI	CASTG	7 cut(s)	138, 415, 523, 887, 1584, 1756, 2122
Van91I	CCANNNNNTGG	4 cut(s)	133, 203, 737, 1646
VpaK11BI	GGWCC	4 cut(s)	728, 1067, 1516, 2393
XapI	RAATTY	9 cut(s)	79, 256, 787, 809, 856, 978, 1157, 2308, 2382
XbaI	TCTAGA	3 cut(s)	1153, 2266, 2501
XceI	RCATGY	7 cut(s)	254, 1209, 1284, 1459, 2535, 2539, 2746
XmnI	GAANNNNTTC	3 cut(s)	236, 459, 2262
XspI	CTAG	7 cut(s)	212, 414, 1154, 2154, 2160, 2267, 2502
ZrmI	AGTACT	1 cut(s)	265
Zsp2I	ATGCAT	1 cut(s)	2537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.