Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002142

Physical Location & Seq

Genomic Coordinates Reverse (-)

37100248 .. 37110072

Gene Structure 9825 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1333600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3816 bp

ATGGCAGAGGACGGCCTACGAATTGGTCGGCTTTCTTCTGGTCTAGCTGTGTTATTGAATGGCGAAGGCAGTAAAGAGAACTCGTCGAAAACCCATCTTGTTTCAAATTGTGATGATTTTGGCCATCAATCGGTGGAGCGTACCTTAGAGTATGTTCTTGGTCTCCCTAACAAATCTGTTGGTCCGCTGGCCAGTCCAATTGACAGCAATCTAGTTCGTCGTTTGATACAGAGTGAATTCTCTAAATTGCATGCAAGTTCAAGTGTGTTTAGAAACAGAGATGGAGTTTGTATTTCTGGAGATGGCTGTGCGCCCCACATCATTGGTCTTGATGAATCCAGTATTCGTGGTGATATTAGAGTTATGAAGCCACCTTTGCTTGTAGAGAGCTTGGCGATGTTCAGCAGTGCCAGGGCTAATGCTTTTGTGTGGAAAGGTAAATGGATGTATGAAGTTATTCTAGAAACTTCAGGTATTCAGCAGCTTGGTTGGGCAACTGTTTCTTGCCCTTTCACTGACCATAAGGGTGTAGGTGATGCTGATGATTCATATGCATTTGATGGGAGGAGGGTTAGAAAATGGAACAAGGAAGCTGAGCCTTATGGTCAGTCATGGGTTGTTGGTGATGCCATTGGCTGCTGCATAGATTTGGATTGTAATGAAATATCATTCTACAGAAATGGTGTTTCACTTGGGCCCGCATTTCATGGCATTCGTAAGATGGGACCAGGATCTGGATATTATCCTGCAGTTTCTCTGTCTCAGGGCGAAAGATGTGAATTAAATTTTGGGGCCCGCCCCTTTAGATTCCCCATTGAAAGCTATTTCCCCCTTCAAGCACCTCCATCCTTGGTTCCGTTTGCTACTCAGTTGCTGTATTGCTTGTCAAGGCTGTTGGGTATGCACTCGGTGGAGCAGGCAAATCACTCTTCTGTTCAGAAGTTGAGGATACTGAAGAGGTTTGTTTCACATGAAGAACTTTTTTATCCTGTTTCTCATGGTATATGTGAGGAATTTTTCTCAGTACTTGGAGCAGATGTGTGGAGCAGAGAGTACATTGCCTGGGGTCCATTTCTATCATTCATGATGGAAGTGTTCGGGTTGCAGATTCCACATGATTATTCAAGTTTAGATAGAGTTCTTGATGTCTTTCTAGAATTTGAAGGATCACATTTGTTGTTTGAACATTTCATAAATGCCTTGGCTTGTGCTTGCAAAACAACTCTGCTGGTTCTAAAAGACTGCCCATGTTCAGGGTCGTACCCATACCTTGCACTGGCATGCCACATCCTGCAACAACAAGAACTTATGGTGCTTTGGTGGAAATCACCTGATTTTGAATTCCTTTTTGAAGGTTTTCTGTCACGGAAGAATCCAAACAAGCATGACCTTGAATCCATGATACCATCTGTTTCTTGGCCTGGTTCAAGTGAGGATGTGTCATATGAAAGTAGCATGTTGTTGACAACTAGAGCTTTATCTGAAGCTGTCAGTAAGATTGAGGAGAAGCATAGGGATCTCTGTCGCTTGGTCATACAGTTTATACCACCTGTGACACCTCCCCAGTTTCCTGGTTCAGTGTTCAGAACTTTTTTACAGAACCTTATAATAAAGAATAGAGGGTCCGACCGCAATCTTCCACCTCCTGGAGTTTCAAGCAATTCTGTTCTGGTTTCTTTGTACACAGTCATACTTCATTTCTTATCCGAAGGGTTTGGCATGGGCGATATTTGCGGCTGGTTGAAAGGCACTGAAAATGGCCCTGATGTTGGTTTTCTTCATAGGGGTGGTCACCGAAGCTTTCCTGTAGGTCTATTTCTTAGAAATGATCCTCATCGAAATGACAGTTCTAGGCTTGGAGGGTCGTTTAGTCATCTTTCAAAATCATACCGGGCATATGATGAGGAAACAGAACTAATTCGGTGGGAGGAAGGCTGTATGGACGATGAAGAAACCAGAGTGACTCATTCAACTACAAAAAAACCATGTTGTTGCTCATGCTACAATGATGATTTTACAAGAATCTCAAAGTATCCAATTCGATACACAGCCAAAGGTTCTCGTGTCCATTGCAGTTCCATTCCAGAGAGATCTGCCCATGTTGCTACAGAATGCAGTACTGGAAGTTTGAATGATGAAATAGAAGATAAAGCTAGTTCTAGTTATCAATCTGAATCTGAATTTAGTTATCACCCAGTGCAGCATATGAGGTTTGTACCAAGGGAAAGTAATATGTCTTCAGCTACGCTCAGAGAAGAAGAACTTCTAGACGTTTTGCTGCTGTTGTATCATATAGGTCTTGCACCAAATTTTAAGCAGGCATCATATTACATGTCTCACCAGTCACAGTCAATATCCCTCATGGAAGAAGCTGATAAACAATTAAGAGAAAAAACTAGCAGTGAGCAATTAAAGCATTTGAAAGAAGCTCGTAATGGTTATAGAGAAGAAGTTATTGATTGTGTCAGACAATGTGCTTGGTACCGTATTTCTCTATTTTCTCGATGGAAGCAAAGAGGAATGTATGCAACATGCATATGGACCGTCCAGTTGCTTTTGGTTCTTAGCAAAGTCGATCTGCTGTTCCTTTATATCCCTGAATATTATCTGGAAGCTCTTGTCGATTGCTTCCATGTATTGCGCAAAAGTGATCCTCCATTTGTACCTTCATCGATATTTATCAAGCAAGGCCTTGCTTCATTTGTCACTTTTGTGGTCACGCACTTCAACGATCCTCGAATATCGAGTGCAGATCTTAGGGATCTGCTTCTCCAATCAATCTCAGTCTTGGTACAGTACAAAGAGTATTTGTCTGTTTTTGAGAGTAATGAAGCAGCCACCCAGAGGATGCCTAAGGCATTGCTGTCAGCATTTGATAATAGATCCTGGATTCCTGTGACGAACATTCTTCTGCGTTTGTGCAAGGGATCTGGCTTTGGTTCTTCAAAGCATGGAGAATCGTCATCTTCATCTGTTGTCTTCCAGAGATTGTTAGGAGAAACATGTATCAATGATGAGGAATTGTTCTCAGCTTTTCTCAATCGCCTATTTAACACTCTCAGCTGGACAATGACTGAATTCTCAATTTCTGTCAGAGAAATGCAAGAGAAGTACCAGGTATTAGAGTTCCAGCAAAAGAAATGCAGTGTCATATTTGATCTTTCATGCAATCTTGCAAGGCTTTTGGAGTTCTGTACCCACGCGATACCTCAAGCATTTCTGTTTGGAGCTGAAACAAACCTTCGAAGGTTGACTGAGTTGATTGTCTTTATTCTGAACCACATAACATCAGCAGAAGATGCCGAGTTTTTTGACTTGTTGCTGAGACGACATGGCCAATCTTTAGAAAAAGTAAACCGAGGGATGATATTGGCACCTCTTGTAGGAATTATCTTAAACTTGTTAAATGCCGGTGAGCATATGGAATGCAGGGAGCACAATGATGTTGTGAGCATCTTTGCAAGTATGGGTTGCCTTGACAGTTTTCAATGCAGATTCCAGTACCTATTGGATTACAACTGGGCTGGAACCTTTAGAGGCGATGCTTATCTTGTAAAACTTGCCCAGCTAGAGAATTTTCTGAGCCTCCTCAGCCGGAGCCAATCACAAGAAAATACAATATGCGAGGGAGAAACGGATGGGGATGATGGCATGTGCTGCATCTGTTATGCCCGTGAAGCAGATGCTCAGTTTTCACCTTGTTCCCACAGGTCATGTTATGGCTGCATAACCAGGCATCTTTTGAATTGCCACAGGTGCTTTTTTTGCAATGCAAGAGTCATGGACGTTGTCCGGATCAGCGAGAAGACGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030163 GO:0032446 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0060154 GO:0065007 GO:0070647 GO:0071704 GO:0098586 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPRY Ufd2P_core zf-C3HC4_3

Protein Analysis

1272

Amino Acids

143.67

Weight (kDa)

5.92

Isoelectric Point (pI)

49.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RKP_N	PF19322	1 - 136	1.8e-59	E3 ubiquitin-protein ligase RKP N-terminus
SPRY	PF00622	147 - 265	1.7e-30	SPRY domain
Ufd2P_core	PF10408	763 - 958	7.4e-06	Ubiquitin elongating factor core
TPR_RNF123	PF25576	1010 - 1202	1.2e-81	RNF123-like, TPR repeat
zf-C3HC4_3	PF13920	1216 - 1261	5.6e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22010 AT2G22010
fragaria_vesca	FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_7g04730 FvH4_7g04730
malus_domestica	MD01G1017400.v1.1 MD15G1333600.v1.1
prunus_persica	Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1
pyrus_communis	pycom01g04820 pycom01g04830 pycom15g29160 pycom15g29170
rosa_chinensis	RchiOBHm_Chr2g0116231
rosa_laevigata	RLG00000018249
rosa_multiflora	Rmu_sc0003499.1_g000012 Rmu_sc0004888.1_g000064
rosa_roxburghii	Rroxscaffold_2G00127410
rosa_rugosa	Rorug02G0205000
rosa_samantha	Rh2AG260600 Rh2BG271700 Rh2CG266800 Rh2DG268800
rosa_wichuraiana	Rw2G020430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1607
Acc16I	TGCGCA	1 cut(s)	2642
Acc65I	GGTACC	1 cut(s)	2481
AccB1I	GGYRCC	2 cut(s)	2481, 3373
AccB7I	CCANNNNNTGG	2 cut(s)	734, 1646
AccII	CGCG	1 cut(s)	3203
AccIII	TCCGGA	1 cut(s)	3792
AciI	CCGC	5 cut(s)	185, 699, 796, 1630, 1734
AcoI	YGGCCR	3 cut(s)	121, 189, 3334
AcsI	RAATTY	9 cut(s)	236, 784, 1013, 1157, 1340, 2180, 2308, 3077, 3574
AcuI	CTGAAG	4 cut(s)	453, 974, 1503, 2223
AdeI	CACNNNGTG	2 cut(s)	910, 2197
AfiI	CCNNNNNNNGG	8 cut(s)	130, 734, 1253, 1276, 1646, 1769, 2844, 3383
AflIII	ACRYGT	2 cut(s)	2331, 3002
AjnI	CCWGG	9 cut(s)	410, 727, 1061, 1420, 1570, 1645, 2885, 3114, 3731
AjuI	GAANNNNNNNTTGG	2 cut(s)	771, 803
AleI	CACNNNNGTG	1 cut(s)	2711
Alw21I	GWGCWC	1 cut(s)	3438
Alw26I	GTCTC	4 cut(s)	167, 765, 2340, 3319
AlwNI	CAGNNNCTG	2 cut(s)	734, 874
Aor13HI	TCCGGA	1 cut(s)	3792
AoxI	GGCC	9 cut(s)	13, 121, 189, 695, 792, 1418, 1759, 2689, 3334
ApaI	GGGCCC	2 cut(s)	699, 796
ApeKI	GCWGC	8 cut(s)	481, 636, 639, 2200, 2278, 2834, 3657, 3723
ApoI	RAATTY	9 cut(s)	236, 784, 1013, 1157, 1340, 2180, 2308, 3077, 3574
Asp700I	GAANNNNTTC	4 cut(s)	456, 1356, 1917, 2262
Asp718I	GGTACC	1 cut(s)	2481
AspLEI	GCGC	2 cut(s)	313, 2643
AsuC2I	CCSGG	1 cut(s)	1892
AsuHPI	GGTGA	9 cut(s)	362, 545, 635, 1320, 1784, 2183, 2330, 3425, 3687
AsuII	TTCGAA	1 cut(s)	3244
AvaII	GGWCC	5 cut(s)	182, 725, 1067, 1623, 2541
AxyI	CCTNAGG	1 cut(s)	2853
BaeGI	GKGCMC	2 cut(s)	699, 796
BalI	TGGCCA	3 cut(s)	123, 191, 3336
BanI	GGYRCC	2 cut(s)	2481, 3373
BanII	GRGCYC	2 cut(s)	699, 796
BarI	GAAGNNNNNNTAC	2 cut(s)	2221, 2253
BauI	CACGAG	1 cut(s)	2061
BbsI	GAAGAC	3 cut(s)	2229, 2971, 3812
Bbv12I	GWGCWC	1 cut(s)	3438
BbvCI	CCTCAGC	1 cut(s)	3590
BbvI	GCAGC	8 cut(s)	493, 623, 626, 2212, 2265, 2846, 3644, 3710
BceAI	ACGGC	1 cut(s)	28
BciT130I	CCWGG	9 cut(s)	412, 729, 1063, 1422, 1572, 1647, 2887, 3116, 3733
BciVI	GTATCC	2 cut(s)	942, 2043
BcnI	CCSGG	1 cut(s)	1892
BcoDI	GTCTC	4 cut(s)	167, 765, 2340, 3319
BfmI	CTRYAG	4 cut(s)	673, 747, 1806, 2106
BfuI	GTATCC	2 cut(s)	942, 2043
BglII	AGATCT	2 cut(s)	2090, 2752
BisI	GCNGC	9 cut(s)	482, 637, 640, 1735, 2201, 2279, 2835, 3658, 3724
BlpI	GCTNAGC	1 cut(s)	594
BlsI	GCNGC	9 cut(s)	483, 638, 641, 1736, 2202, 2280, 2836, 3659, 3725
BmcAI	AGTACT	2 cut(s)	1026, 2119
Bme18I	GGWCC	5 cut(s)	182, 725, 1067, 1623, 2541
BmrI	ACTGGG	3 cut(s)	1558, 2189, 3529
BmuI	ACTGGG	3 cut(s)	1558, 2189, 3529
BpiI	GAAGAC	3 cut(s)	2229, 2971, 3812
BpmI	CTGGAG	2 cut(s)	318, 1668
Bpu10I	CCTNAGC	1 cut(s)	3590
Bpu1102I	GCTNAGC	1 cut(s)	594
Bpu14I	TTCGAA	1 cut(s)	3244
BpuEI	CTTGAG	1 cut(s)	3195
BpuMI	CCSGG	1 cut(s)	1892
Bsa29I	ATCGAT	1 cut(s)	2672
BsaBI	GATNNNNATC	3 cut(s)	1833, 2678, 3546
BsaI	GGTCTC	1 cut(s)	167
BsaJI	CCNNGG	6 cut(s)	411, 849, 1062, 1200, 2219, 3358
BsaWI	WCCGGW	1 cut(s)	3792
BsaXI	ACNNNNNCTCC	2 cut(s)	1023, 1053
Bsc4I	CCNNNNNNNGG	8 cut(s)	130, 734, 1253, 1276, 1646, 1769, 2844, 3383
Bse118I	RCCGGY	1 cut(s)	3410
Bse21I	CCTNAGG	1 cut(s)	2853
Bse3DI	GCAATG	4 cut(s)	1056, 2068, 2858, 3775
Bse8I	GATNNNNATC	3 cut(s)	1833, 2678, 3546
BseAI	TCCGGA	1 cut(s)	3792
BseBI	CCWGG	9 cut(s)	412, 729, 1063, 1422, 1572, 1647, 2887, 3116, 3733
BseCI	ATCGAT	1 cut(s)	2672
BseDI	CCNNGG	6 cut(s)	411, 849, 1062, 1200, 2219, 3358
BseGI	GGATG	8 cut(s)	450, 845, 1287, 1441, 2853, 3369, 3643, 3649
BseJI	GATNNNNATC	3 cut(s)	1833, 2678, 3546
BseLI	CCNNNNNNNGG	8 cut(s)	130, 734, 1253, 1276, 1646, 1769, 2844, 3383
BseMI	GCAATG	4 cut(s)	1056, 2068, 2858, 3775
BseRI	GAGGAG	3 cut(s)	580, 1517, 3578
BseSI	GKGCMC	2 cut(s)	699, 796
BseXI	GCAGC	8 cut(s)	493, 623, 626, 2212, 2265, 2846, 3644, 3710
BseYI	CCCAGC	1 cut(s)	3564
BsgI	GTGCAG	2 cut(s)	2219, 2769
Bsh1236I	CGCG	1 cut(s)	3203
Bsh1285I	CGRYCG	1 cut(s)	1630
BshFI	GGCC	9 cut(s)	15, 123, 191, 697, 794, 1420, 1761, 2691, 3336
BshNI	GGYRCC	2 cut(s)	2481, 3373
BshVI	ATCGAT	1 cut(s)	2672
BsiEI	CGRYCG	1 cut(s)	1630
BsiHKAI	GWGCWC	1 cut(s)	3438
BsiSI	CCGG	4 cut(s)	1891, 3411, 3595, 3793
BslFI	GGGAC	1 cut(s)	738
BslI	CCNNNNNNNGG	8 cut(s)	130, 734, 1253, 1276, 1646, 1769, 2844, 3383
BsmAI	GTCTC	4 cut(s)	167, 765, 2340, 3319
BsmBI	CGTCTC	1 cut(s)	3319
BsmFI	GGGAC	1 cut(s)	738
BsmI	GAATGC	3 cut(s)	711, 2117, 3431
BsnI	GGCC	9 cut(s)	15, 123, 191, 697, 794, 1420, 1761, 2691, 3336
Bso31I	GGTCTC	1 cut(s)	167
Bsp119I	TTCGAA	1 cut(s)	3244
Bsp120I	GGGCCC	2 cut(s)	695, 792
Bsp1286I	GDGCHC	3 cut(s)	699, 796, 3438
Bsp13I	TCCGGA	1 cut(s)	3792
Bsp1407I	TGTACA	1 cut(s)	1680
Bsp1720I	GCTNAGC	1 cut(s)	594
BspACI	CCGC	5 cut(s)	185, 699, 796, 1630, 1734
BspANI	GGCC	9 cut(s)	15, 123, 191, 697, 794, 1420, 1761, 2691, 3336
BspDI	ATCGAT	1 cut(s)	2672
BspEI	TCCGGA	1 cut(s)	3792
BspFNI	CGCG	1 cut(s)	3203
BspHI	TCATGA	1 cut(s)	1083
BspMAI	CTGCAG	1 cut(s)	751
BspT104I	TTCGAA	1 cut(s)	3244
BspT107I	GGYRCC	2 cut(s)	2481, 3373
BspTNI	GGTCTC	1 cut(s)	167
BsrDI	GCAATG	4 cut(s)	1056, 2068, 2858, 3775
BsrFI	RCCGGY	1 cut(s)	3410
BsrGI	TGTACA	1 cut(s)	1680
BssAI	RCCGGY	1 cut(s)	3410
BssECI	CCNNGG	6 cut(s)	411, 849, 1062, 1200, 2219, 3358
BssSI	CACGAG	1 cut(s)	2061
BssT1I	CCWWGG	3 cut(s)	849, 1200, 2219
Bst2BI	CACGAG	1 cut(s)	2061
Bst2UI	CCWGG	9 cut(s)	412, 729, 1063, 1422, 1572, 1647, 2887, 3116, 3733
Bst4CI	ACNGT	9 cut(s)	499, 1539, 1687, 1847, 2349, 2486, 2545, 2796, 3482
Bst6I	CTCTTC	2 cut(s)	934, 950
BstAPI	GCANNNNNTGC	2 cut(s)	3299, 3657
BstAUI	TGTACA	1 cut(s)	1680
BstBI	TTCGAA	1 cut(s)	3244
BstC8I	GCNNGC	8 cut(s)	189, 252, 699, 796, 918, 1213, 1282, 2319
BstEII	GGTNACC	1 cut(s)	1790
BstENI	CCTNNNNNAGG	1 cut(s)	3381
BstF5I	GGATG	8 cut(s)	450, 845, 1287, 1441, 2853, 3369, 3643, 3649
BstFNI	CGCG	1 cut(s)	3203
BstHHI	GCGC	2 cut(s)	313, 2643
BstMAI	GTCTC	4 cut(s)	167, 765, 2340, 3319
BstMCI	CGRYCG	1 cut(s)	1630
BstNI	CCWGG	9 cut(s)	412, 729, 1063, 1422, 1572, 1647, 2887, 3116, 3733
BstNSI	RCATGY	7 cut(s)	254, 1284, 1459, 2335, 2535, 3006, 3655
BstPI	GGTNACC	1 cut(s)	1790
BstSFI	CTRYAG	4 cut(s)	673, 747, 1806, 2106
BstSLI	GKGCMC	2 cut(s)	699, 796
BstUI	CGCG	1 cut(s)	3203
BstV1I	GCAGC	8 cut(s)	493, 623, 626, 2212, 2265, 2846, 3644, 3710
BstV2I	GAAGAC	3 cut(s)	2229, 2971, 3812
BstX2I	RGATCY	7 cut(s)	731, 1516, 2090, 2752, 2761, 2882, 2927
BstXI	CCANNNNNNTGG	2 cut(s)	323, 1571
BstYI	RGATCY	7 cut(s)	731, 1516, 2090, 2752, 2761, 2882, 2927
Bsu15I	ATCGAT	1 cut(s)	2672
Bsu36I	CCTNAGG	1 cut(s)	2853
BsuI	GTATCC	2 cut(s)	942, 2043
BsuRI	GGCC	9 cut(s)	15, 123, 191, 697, 794, 1420, 1761, 2691, 3336
BsuTUI	ATCGAT	1 cut(s)	2672
BtgZI	GCGATG	2 cut(s)	410, 3555
BtsCI	GGATG	8 cut(s)	450, 845, 1287, 1441, 2853, 3369, 3643, 3649
BtsI	GCAGTG	3 cut(s)	412, 2407, 3151
BtsIMutI	CAGTG	8 cut(s)	412, 513, 1274, 1584, 1749, 2202, 2407, 3151
Cac8I	GCNNGC	8 cut(s)	189, 252, 699, 796, 918, 1213, 1282, 2319
CaiI	CAGNNNCTG	2 cut(s)	734, 874
CciI	TCATGA	1 cut(s)	1083
CfoI	GCGC	2 cut(s)	313, 2643
Cfr10I	RCCGGY	1 cut(s)	3410
ClaI	ATCGAT	1 cut(s)	2672
CsiI	ACCWGGT	1 cut(s)	3114
DraIII	CACNNNGTG	2 cut(s)	910, 2197
EaeI	YGGCCR	3 cut(s)	121, 189, 3334
Eam1104I	CTCTTC	2 cut(s)	934, 950
EarI	CTCTTC	2 cut(s)	934, 950
Eco130I	CCWWGG	3 cut(s)	849, 1200, 2219
Eco147I	AGGCCT	1 cut(s)	2691
Eco24I	GRGCYC	2 cut(s)	699, 796
Eco31I	GGTCTC	1 cut(s)	167
Eco47I	GGWCC	5 cut(s)	182, 725, 1067, 1623, 2541
Eco57I	CTGAAG	4 cut(s)	453, 974, 1503, 2223
Eco81I	CCTNAGG	1 cut(s)	2853
Eco91I	GGTNACC	1 cut(s)	1790
EcoNI	CCTNNNNNAGG	1 cut(s)	3381
EcoO109I	RGGNCCY	1 cut(s)	792
EcoO65I	GGTNACC	1 cut(s)	1790
EcoRI	GAATTC	3 cut(s)	236, 1340, 3077
EcoRII	CCWGG	9 cut(s)	410, 727, 1061, 1420, 1570, 1645, 2885, 3114, 3731
EcoT14I	CCWWGG	3 cut(s)	849, 1200, 2219
EcoT22I	ATGCAT	2 cut(s)	556, 2537
EcoT38I	GRGCYC	2 cut(s)	699, 796
ErhI	CCWWGG	3 cut(s)	849, 1200, 2219
Esp3I	CGTCTC	1 cut(s)	3319
FalI	AAGNNNNNCTT	2 cut(s)	2247, 2279
FaqI	GGGAC	1 cut(s)	738
FauI	CCCGC	2 cut(s)	706, 803
FauNDI	CATATG	6 cut(s)	550, 1444, 1897, 2205, 2537, 3420
Fnu4HI	GCNGC	9 cut(s)	482, 637, 640, 1735, 2201, 2279, 2835, 3658, 3724
FokI	GGATG	8 cut(s)	457, 832, 1274, 1448, 2860, 3376, 3650, 3656
FriOI	GRGCYC	2 cut(s)	699, 796
Fsp4HI	GCNGC	9 cut(s)	482, 637, 640, 1735, 2201, 2279, 2835, 3658, 3724
FspI	TGCGCA	1 cut(s)	2642
GlaI	GCGC	2 cut(s)	312, 2642
GluI	GCNGC	9 cut(s)	482, 637, 640, 1735, 2201, 2279, 2835, 3658, 3724
GsaI	CCCAGC	1 cut(s)	3568
GsuI	CTGGAG	2 cut(s)	318, 1668
HaeIII	GGCC	9 cut(s)	15, 123, 191, 697, 794, 1420, 1761, 2691, 3336
HapII	CCGG	4 cut(s)	1891, 3411, 3595, 3793
HhaI	GCGC	2 cut(s)	313, 2643
Hin6I	GCGC	2 cut(s)	311, 2641
HinP1I	GCGC	2 cut(s)	311, 2641
HincII	GTYRAC	2 cut(s)	1464, 3252
HindII	GTYRAC	2 cut(s)	1464, 3252
HindIII	AAGCTT	1 cut(s)	1798
HpaII	CCGG	4 cut(s)	1891, 3411, 3595, 3793
HphI	GGTGA	9 cut(s)	362, 545, 635, 1320, 1784, 2183, 2330, 3425, 3687
Hpy166II	GTNNAC	4 cut(s)	1464, 1683, 3252, 3355
Hpy8I	GTNNAC	4 cut(s)	1464, 1683, 3252, 3355
Hpy99I	CGWCG	2 cut(s)	88, 222
HpyAV	CCTTC	9 cut(s)	59, 842, 1157, 1346, 1703, 1925, 2676, 3240, 3251
HpyCH4III	ACNGT	9 cut(s)	499, 1539, 1687, 1847, 2349, 2486, 2545, 2796, 3482
HpyCH4IV	ACGT	2 cut(s)	2271, 3786
HpySE526I	ACGT	2 cut(s)	2271, 3786
HspAI	GCGC	2 cut(s)	311, 2641
Kpn2I	TCCGGA	1 cut(s)	3792
KpnI	GGTACC	1 cut(s)	2485
LmnI	GCTCC	7 cut(s)	136, 913, 1031, 1044, 3227, 3433, 3597
Lsp1109I	GCAGC	8 cut(s)	493, 623, 626, 2212, 2265, 2846, 3644, 3710
MabI	ACCWGGT	1 cut(s)	3114
MaeII	ACGT	2 cut(s)	2271, 3786
MaeIII	GTNAC	8 cut(s)	1362, 1552, 1790, 1960, 2343, 2704, 2716, 2896
MfeI	CAATTG	1 cut(s)	198
MflI	RGATCY	7 cut(s)	731, 1516, 2090, 2752, 2761, 2882, 2927
MhlI	GDGCHC	3 cut(s)	699, 796, 3438
MlsI	TGGCCA	3 cut(s)	123, 191, 3336
MluNI	TGGCCA	3 cut(s)	123, 191, 3336
MlyI	GAGTC	2 cut(s)	1957, 3786
MmeI	TCCRAC	1 cut(s)	1650
Mox20I	TGGCCA	3 cut(s)	123, 191, 3336
Mph1103I	ATGCAT	2 cut(s)	556, 2537
MroI	TCCGGA	1 cut(s)	3792
MroXI	GAANNNNTTC	4 cut(s)	456, 1356, 1917, 2262
MscI	TGGCCA	3 cut(s)	123, 191, 3336
MseI	TTAA	7 cut(s)	782, 2313, 2384, 2411, 3051, 3395, 3404
MslI	CAYNNNNRTG	7 cut(s)	525, 1279, 1785, 1839, 2536, 2711, 3441
Msp20I	TGGCCA	3 cut(s)	123, 191, 3336
MspA1I	CMGCKG	2 cut(s)	187, 3063
MspI	CCGG	4 cut(s)	1891, 3411, 3595, 3793
MunI	CAATTG	1 cut(s)	198
Mva1269I	GAATGC	3 cut(s)	711, 2117, 3431
MvaI	CCWGG	9 cut(s)	412, 729, 1063, 1422, 1572, 1647, 2887, 3116, 3733
MvnI	CGCG	1 cut(s)	3203
NciI	CCSGG	1 cut(s)	1892
NdeI	CATATG	6 cut(s)	550, 1444, 1897, 2205, 2537, 3420
NmeAIII	GCCGAG	1 cut(s)	3328
NmuCI	GTSAC	8 cut(s)	1362, 1552, 1790, 1960, 2343, 2704, 2716, 2896
NsbI	TGCGCA	1 cut(s)	2642
NsiI	ATGCAT	2 cut(s)	556, 2537
NspI	RCATGY	7 cut(s)	254, 1284, 1459, 2335, 2535, 3006, 3655
NspV	TTCGAA	1 cut(s)	3244
OliI	CACNNNNGTG	1 cut(s)	2711
PaeI	GCATGC	2 cut(s)	254, 1284
PagI	TCATGA	1 cut(s)	1083
PceI	AGGCCT	1 cut(s)	2691
PciI	ACATGT	2 cut(s)	2331, 3002
PctI	GAATGC	3 cut(s)	711, 2117, 3431
PdmI	GAANNNNTTC	4 cut(s)	456, 1356, 1917, 2262
PflFI	GACNNNGTC	1 cut(s)	3788
PflMI	CCANNNNNTGG	2 cut(s)	734, 1646
PfoI	TCCNGGA	2 cut(s)	1645, 2885
PkrI	GCNGC	9 cut(s)	483, 638, 641, 1736, 2202, 2280, 2836, 3659, 3725
PleI	GAGTC	2 cut(s)	1957, 3785
PpsI	GAGTC	2 cut(s)	1957, 3785
PscI	ACATGT	2 cut(s)	2331, 3002
PsiI	TTATAA	1 cut(s)	1607
Psp6I	CCWGG	9 cut(s)	410, 727, 1061, 1420, 1570, 1645, 2885, 3114, 3731
PspEI	GGTNACC	1 cut(s)	1790
PspFI	CCCAGC	1 cut(s)	3564
PspGI	CCWGG	9 cut(s)	410, 727, 1061, 1420, 1570, 1645, 2885, 3114, 3731
PspOMI	GGGCCC	2 cut(s)	695, 792
PstI	CTGCAG	1 cut(s)	751
PstNI	CAGNNNCTG	2 cut(s)	734, 874
PsuI	RGATCY	7 cut(s)	731, 1516, 2090, 2752, 2761, 2882, 2927
PsyI	GACNNNGTC	1 cut(s)	3788
PvuII	CAGCTG	1 cut(s)	3063
RseI	CAYNNNNRTG	7 cut(s)	525, 1279, 1785, 1839, 2536, 2711, 3441
SaqAI	TTAA	7 cut(s)	782, 2313, 2384, 2411, 3051, 3395, 3404
SatI	GCNGC	9 cut(s)	482, 637, 640, 1735, 2201, 2279, 2835, 3658, 3724
ScaI	AGTACT	2 cut(s)	1026, 2119
SchI	GAGTC	2 cut(s)	1957, 3786
SduI	GDGCHC	3 cut(s)	699, 796, 3438
SexAI	ACCWGGT	1 cut(s)	3114
SfcI	CTRYAG	4 cut(s)	673, 747, 1806, 2106
SfuI	TTCGAA	1 cut(s)	3244
SinI	GGWCC	5 cut(s)	182, 725, 1067, 1623, 2541
SmiMI	CAYNNNNRTG	7 cut(s)	525, 1279, 1785, 1839, 2536, 2711, 3441
SmlI	CTYRAG	1 cut(s)	3210
SmoI	CTYRAG	1 cut(s)	3210
SphI	GCATGC	2 cut(s)	254, 1284
SseBI	AGGCCT	1 cut(s)	2691
SsiI	CCGC	5 cut(s)	185, 699, 796, 1630, 1734
SspI	AATATT	1 cut(s)	2603
StuI	AGGCCT	1 cut(s)	2691
StyI	CCWWGG	3 cut(s)	849, 1200, 2219
TaaI	ACNGT	9 cut(s)	499, 1539, 1687, 1847, 2349, 2486, 2545, 2796, 3482
TaiI	ACGT	2 cut(s)	2274, 3789
TatI	WGTACW	5 cut(s)	1024, 1053, 1680, 2117, 2796
TauI	GCSGC	1 cut(s)	1737
Tru1I	TTAA	7 cut(s)	782, 2313, 2384, 2411, 3051, 3395, 3404
Tru9I	TTAA	7 cut(s)	782, 2313, 2384, 2411, 3051, 3395, 3404
TscAI	CASTG	8 cut(s)	412, 520, 1281, 1584, 1756, 2202, 2407, 3151
TseFI	GTSAC	8 cut(s)	1362, 1552, 1790, 1960, 2343, 2704, 2716, 2896
TseI	GCWGC	8 cut(s)	481, 636, 639, 2200, 2278, 2834, 3657, 3723
Tsp45I	GTSAC	8 cut(s)	1362, 1552, 1790, 1960, 2343, 2704, 2716, 2896
TspGWI	ACGGA	3 cut(s)	846, 1381, 3650
TspRI	CASTG	8 cut(s)	412, 520, 1281, 1584, 1756, 2202, 2407, 3151
Tth111I	GACNNNGTC	1 cut(s)	3788
Van91I	CCANNNNNTGG	2 cut(s)	734, 1646
VpaK11BI	GGWCC	5 cut(s)	182, 725, 1067, 1623, 2541
XagI	CCTNNNNNAGG	1 cut(s)	3381
XapI	RAATTY	9 cut(s)	236, 784, 1013, 1157, 1340, 2180, 2308, 3077, 3574
XbaI	TCTAGA	3 cut(s)	460, 1153, 2266
XceI	RCATGY	7 cut(s)	254, 1284, 1459, 2335, 2535, 3006, 3655
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1413
XmnI	GAANNNNTTC	4 cut(s)	456, 1356, 1917, 2262
ZrmI	AGTACT	2 cut(s)	1026, 2119
Zsp2I	ATGCAT	2 cut(s)	556, 2537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.