AT2G23430

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009816
Physical Location & Seq
Reverse (-)
9976590 .. 9978090
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G23430.1

Sequence Viewer

Length: 576 bp
ATGGTGAGAAAATATAGAAAAGCTAAAGGAATTGTAGAAGCTGGAGTTTCGTCAACGTATATGCAGCTACGGAGCCGGAGAATTGTTTATGTTAGATCGGAAAAATCAAGCTCTGTCTCCGTCGTCGGTGATAATGGAGTTTCGTCGTCTTGTAGTGGAAGCAATGAATATAAGAAGAAAGAATTAATACATCTGGAGGAGGAAGATAAAGATGGTGACACTGAAACGTCGACGTATCGACGGGGTACGAAGAGGAAGCTTTTTGAAAATCTGAGAGAGGAGGAGAAAGAAGAATTAAGTAAATCCATGGAGAATTATTCATCGGAATTTGAATCGGCGGTTAAAGAATCGTTAGATTGTTGTTGTAGCGGGAGGAAAACGATGGAGGAGACGGTGACGGCGGAGGAGGAGGAGAAGGCGAAATTGATGACGGAGATGCCAACGGAATCGGAAATTGAAGATTTTTTTGTGGAAGCTGAGAAACAACTCAAAGAAAAATTCAAGAAGAAGTACAATTTCGATTTCGAGAAGGAGAAGCCATTAGAAGGACGTTACGAATGGGTAAAGTTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

191

Amino Acids

22.28

Weight (kDa)

5.29

Isoelectric Point (pI)

54.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 146 - 190 2.2e-17 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 230
AciI CCGC 3 cut(s) 338, 369, 401
AcsI RAATTY 2 cut(s) 326, 497
AfaI GTAC 2 cut(s) 247, 512
AfiI CCNNNNNNNGG 1 cut(s) 545
AgsI TTSAA 4 cut(s) 266, 332, 458, 502
AluBI AGCT 6 cut(s) 23, 41, 67, 111, 259, 476
AluI AGCT 6 cut(s) 23, 41, 67, 111, 259, 476
Alw26I GTCTC 2 cut(s) 121, 383
ApeKI GCWGC 1 cut(s) 64
ApoI RAATTY 2 cut(s) 326, 497
AseI ATTAAT 1 cut(s) 185
AsuHPI GGTGA 4 cut(s) 16, 140, 227, 406
BbvI GCAGC 1 cut(s) 76
BccI CCATC 2 cut(s) 206, 376
BceAI ACGGC 1 cut(s) 414
BcoDI GTCTC 2 cut(s) 121, 383
BisI GCNGC 1 cut(s) 65
BlsI GCNGC 1 cut(s) 66
BmiI GGNNCC 1 cut(s) 74
BmsI GCATC 1 cut(s) 426
BpmI CTGGAG 2 cut(s) 63, 215
BsaJI CCNNGG 1 cut(s) 306
Bsc4I CCNNNNNNNGG 1 cut(s) 545
Bse3DI GCAATG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 306
BseLI CCNNNNNNNGG 1 cut(s) 545
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 2 cut(s) 263, 468
BseRI GAGGAG 7 cut(s) 212, 293, 296, 401, 419, 422, 425
BseXI GCAGC 1 cut(s) 76
BsiSI CCGG 1 cut(s) 76
BslI CCNNNNNNNGG 1 cut(s) 545
BsmAI GTCTC 2 cut(s) 121, 383
BsmBI CGTCTC 1 cut(s) 383
Bsp143I GATC 1 cut(s) 95
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 3 cut(s) 338, 369, 401
BspCNI CTCAG 2 cut(s) 264, 469
BspLI GGNNCC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 1 cut(s) 245
BstDEI CTNAG 2 cut(s) 272, 477
BstDSI CCRYGG 1 cut(s) 306
BstKTI GATC 1 cut(s) 98
BstMAI GTCTC 2 cut(s) 121, 383
BstMBI GATC 1 cut(s) 95
BstV1I GCAGC 1 cut(s) 76
BtgI CCRYGG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 219
Csp6I GTAC 2 cut(s) 246, 511
CviAII CATG 1 cut(s) 307
CviJI RGCY 8 cut(s) 23, 41, 67, 75, 111, 259, 476, 538
CviKI_1 RGCY 8 cut(s) 23, 41, 67, 75, 111, 259, 476, 538
CviQI GTAC 2 cut(s) 246, 511
DdeI CTNAG 2 cut(s) 272, 477
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
EciI GGCGGA 1 cut(s) 416
Eco130I CCWWGG 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 1 cut(s) 310
FaiI YATR 6 cut(s) 15, 60, 62, 90, 171, 308
FatI CATG 1 cut(s) 306
FauI CCCGC 1 cut(s) 362
FblI GTMKAC 1 cut(s) 230
Fnu4HI GCNGC 1 cut(s) 65
Fsp4HI GCNGC 1 cut(s) 65
GluI GCNGC 1 cut(s) 65
GsuI CTGGAG 2 cut(s) 63, 215
HapII CCGG 1 cut(s) 76
Hin1II CATG 1 cut(s) 310
HincII GTYRAC 2 cut(s) 54, 231
HindII GTYRAC 2 cut(s) 54, 231
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 3 cut(s) 332, 347, 446
HpaII CCGG 1 cut(s) 76
HphI GGTGA 4 cut(s) 16, 140, 227, 406
Hpy166II GTNNAC 2 cut(s) 54, 231
Hpy188I TCNGA 4 cut(s) 100, 273, 325, 451
Hpy188III TCNNGA 3 cut(s) 194, 502, 526
Hpy8I GTNNAC 2 cut(s) 54, 231
Hpy99I CGWCG 6 cut(s) 125, 128, 148, 232, 235, 243
HpyAV CCTTC 3 cut(s) 409, 523, 539
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 4 cut(s) 56, 227, 233, 550
HpyCH4V TGCA 1 cut(s) 64
HpyF3I CTNAG 2 cut(s) 272, 477
HpySE526I ACGT 4 cut(s) 56, 227, 233, 550
Hsp92II CATG 1 cut(s) 310
Kzo9I GATC 1 cut(s) 95
LmnI GCTCC 1 cut(s) 72
LpnPI CCDG 3 cut(s) 27, 89, 179
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 1 cut(s) 426
MaeII ACGT 4 cut(s) 56, 227, 233, 550
MaeIII GTNAC 3 cut(s) 215, 394, 551
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 6 cut(s) 187, 215, 262, 302, 470, 517
MseI TTAA 3 cut(s) 185, 296, 342
MspI CCGG 1 cut(s) 76
NcoI CCATGG 1 cut(s) 306
NdeII GATC 1 cut(s) 95
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 1 cut(s) 74
NmuCI GTSAC 2 cut(s) 215, 394
PfeI GAWTC 3 cut(s) 332, 347, 446
PkrI GCNGC 1 cut(s) 66
PshBI ATTAAT 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 74
RsaI GTAC 2 cut(s) 247, 512
RsaNI GTAC 2 cut(s) 246, 511
SalI GTCGAC 1 cut(s) 229
SaqAI TTAA 3 cut(s) 185, 296, 342
SatI GCNGC 1 cut(s) 65
Sau3AI GATC 1 cut(s) 95
SfaNI GCATC 1 cut(s) 426
SgrDI CGTCGACG 1 cut(s) 229
SsiI CCGC 3 cut(s) 338, 369, 401
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 4 cut(s) 59, 230, 236, 553
TaqI TCGA 4 cut(s) 230, 238, 519, 525
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 3 cut(s) 332, 347, 446
Tru1I TTAA 3 cut(s) 185, 296, 342
Tru9I TTAA 3 cut(s) 185, 296, 342
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 2 cut(s) 215, 394
TseI GCWGC 1 cut(s) 64
Tsp45I GTSAC 2 cut(s) 215, 394
TspDTI ATGAA 2 cut(s) 180, 309
TspGWI ACGGA 4 cut(s) 85, 109, 446, 458
TspRI CASTG 1 cut(s) 226
VspI ATTAAT 1 cut(s) 185
XapI RAATTY 2 cut(s) 326, 497
XmiI GTMKAC 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.