RLG00000017404

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009816
Physical Location & Seq
Forward (+)
16190546 .. 16191668
1123 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017404

Sequence Viewer

Length: 609 bp
ATGGAGGCCAAGACGGCGGAAACCAGCTCAGTCAAGAAGAGGAAGGTGGAAGTTGCACAGTTGGTCAAATCCAAACTCGTTCACCTCGACAAGAAGCCGAGACCTAGTGTTGAAACTATATCGAAAATAGGAGAGGGAAGAGAGTGTACTGATGTTGCTACTGTTCGGAAACCAAAGGAGGAAGATGATGACGCCGTTTGCGCCAGCCCTAGGAGCTCAGGCTTCGATGATCACGTCGACGACACCTCCTGTTGCTCGAGTAACAGCTCGAGTGAGCTGGTGGAAGATGGACATAACCTCAAATTCGTAGATCTGGTGGAGGATGACGGTGATGATGTTGAGAGTGACGAAGTTGAATCGCCGACGTATAATCCACTCAGAGAAAGGAGACAGATGACGCCTTCTAGCAACATTCAACCTCAACCAACGCCTGCGGAACCTCGGCGAGGTTCCACGGCAGAGAAAATGCCTACAGAATTGGAGCTGGAGGAATTCTTCACGGCGGGCGAGAAAGACATCCAAAATAAATTTTCACAGAAATATAACTACGATCTAACGAAGGACGAGCCGTTGGAAGGACGTTACGAATGGACTCGATTAAAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

203

Amino Acids

22.77

Weight (kDa)

4.77

Isoelectric Point (pI)

55.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 155 - 200 7.6e-16 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 237
AciI CCGC 3 cut(s) 17, 434, 503
AcsI RAATTY 3 cut(s) 302, 491, 527
AcyI GRCGYC 2 cut(s) 192, 398
AfaI GTAC 1 cut(s) 148
AfiI CCNNNNNNNGG 3 cut(s) 210, 446, 575
AgsI TTSAA 3 cut(s) 113, 356, 416
AjiI CACGTC 1 cut(s) 235
AluBI AGCT 5 cut(s) 27, 216, 267, 277, 484
AluI AGCT 5 cut(s) 27, 216, 267, 277, 484
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 2 cut(s) 94, 382
Ama87I CYCGRG 2 cut(s) 256, 268
AoxI GGCC 1 cut(s) 6
ApoI RAATTY 3 cut(s) 302, 491, 527
AspA2I CCTAGG 1 cut(s) 209
AspLEI GCGC 1 cut(s) 203
AsuHPI GGTGA 2 cut(s) 74, 341
AvaI CYCGRG 2 cut(s) 256, 268
AvrII CCTAGG 1 cut(s) 209
BanII GRGCYC 1 cut(s) 218
BarI GAAGNNNNNNTAC 2 cut(s) 130, 162
Bbv12I GWGCWC 1 cut(s) 218
BccI CCATC 1 cut(s) 281
BceAI ACGGC 5 cut(s) 30, 179, 471, 516, 553
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 94, 382
BfaI CTAG 3 cut(s) 105, 210, 405
BfmI CTRYAG 1 cut(s) 471
BglI GCCNNNNNGGC 1 cut(s) 14
BglII AGATCT 1 cut(s) 310
BlnI CCTAGG 1 cut(s) 209
BmeT110I CYCGRG 2 cut(s) 256, 268
BmgBI CACGTC 1 cut(s) 235
BmiI GGNNCC 2 cut(s) 438, 451
BpmI CTGGAG 1 cut(s) 506
Bpu10I CCTNAGC 1 cut(s) 217
BsaHI GRCGYC 2 cut(s) 192, 398
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 3 cut(s) 209, 440, 453
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 446, 575
BseDI CCNNGG 3 cut(s) 209, 440, 453
BseGI GGATG 2 cut(s) 328, 516
BseLI CCNNNNNNNGG 3 cut(s) 210, 446, 575
BseMII CTCAG 3 cut(s) 42, 231, 391
BshFI GGCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 218
BsiHKCI CYCGRG 2 cut(s) 256, 268
BslI CCNNNNNNNGG 3 cut(s) 210, 446, 575
BsmAI GTCTC 2 cut(s) 94, 382
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 1 cut(s) 94
BsoBI CYCGRG 2 cut(s) 256, 268
Bsp1286I GDGCHC 1 cut(s) 218
Bsp143I GATC 3 cut(s) 229, 310, 550
BspACI CCGC 3 cut(s) 17, 434, 503
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 3 cut(s) 41, 230, 390
BspLI GGNNCC 2 cut(s) 438, 451
BspTNI GGTCTC 1 cut(s) 94
BssECI CCNNGG 3 cut(s) 209, 440, 453
BssMI GATC 3 cut(s) 229, 310, 550
BssNI GRCGYC 2 cut(s) 192, 398
BssT1I CCWWGG 1 cut(s) 209
Bst4CI ACNGT 3 cut(s) 60, 163, 329
Bst6I CTCTTC 2 cut(s) 32, 133
BstACI GRCGYC 2 cut(s) 192, 398
BstC8I GCNNGC 3 cut(s) 205, 432, 505
BstDEI CTNAG 3 cut(s) 28, 217, 377
BstDSI CCRYGG 1 cut(s) 453
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 2 cut(s) 328, 516
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 3 cut(s) 232, 313, 553
BstMAI GTCTC 2 cut(s) 94, 382
BstMBI GATC 3 cut(s) 229, 310, 550
BstMWI GCNNNNNNNGC 3 cut(s) 14, 200, 213
BstSFI CTRYAG 1 cut(s) 471
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BsuRI GGCC 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 453
BtrI CACGTC 1 cut(s) 235
BtsCI GGATG 2 cut(s) 328, 516
Cac8I GCNNGC 3 cut(s) 205, 432, 505
CfoI GCGC 1 cut(s) 203
CseI GACGC 2 cut(s) 200, 406
Csp6I GTAC 1 cut(s) 147
CviQI GTAC 1 cut(s) 147
DdeI CTNAG 3 cut(s) 28, 217, 377
DpnI GATC 3 cut(s) 231, 312, 552
DpnII GATC 3 cut(s) 229, 310, 550
Eam1104I CTCTTC 2 cut(s) 32, 133
EarI CTCTTC 2 cut(s) 32, 133
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 209
Eco24I GRGCYC 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 216
Eco88I CYCGRG 2 cut(s) 256, 268
EcoICRI GAGCTC 1 cut(s) 216
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 491
EcoT14I CCWWGG 1 cut(s) 209
EcoT38I GRGCYC 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 209
FaiI YATR 5 cut(s) 119, 294, 369, 543, 607
FauI CCCGC 1 cut(s) 496
FbaI TGATCA 1 cut(s) 229
FblI GTMKAC 1 cut(s) 237
FokI GGATG 2 cut(s) 335, 503
FriOI GRGCYC 1 cut(s) 218
FspBI CTAG 3 cut(s) 105, 210, 405
GlaI GCGC 1 cut(s) 202
GsuI CTGGAG 1 cut(s) 506
HaeIII GGCC 1 cut(s) 8
HgaI GACGC 2 cut(s) 200, 406
HhaI GCGC 1 cut(s) 203
Hin1I GRCGYC 2 cut(s) 192, 398
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 2 cut(s) 356, 592
HphI GGTGA 2 cut(s) 74, 341
Hpy166II GTNNAC 3 cut(s) 82, 147, 238
Hpy188I TCNGA 2 cut(s) 168, 380
Hpy188III TCNNGA 1 cut(s) 34
Hpy8I GTNNAC 3 cut(s) 82, 147, 238
Hpy99I CGWCG 3 cut(s) 239, 242, 367
HpyAV CCTTC 4 cut(s) 37, 411, 553, 569
HpyCH4III ACNGT 3 cut(s) 60, 163, 329
HpyCH4IV ACGT 3 cut(s) 234, 365, 580
HpyCH4V TGCA 1 cut(s) 56
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 200, 213
HpyF3I CTNAG 3 cut(s) 28, 217, 377
HpySE526I ACGT 3 cut(s) 234, 365, 580
Hsp92I GRCGYC 2 cut(s) 192, 398
HspAI GCGC 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 3 cut(s) 229, 310, 550
LmnI GCTCC 2 cut(s) 213, 481
LpnPI CCDG 8 cut(s) 37, 204, 217, 262, 263, 299, 444, 470
MaeI CTAG 3 cut(s) 105, 210, 405
MaeII ACGT 3 cut(s) 234, 365, 580
MaeIII GTNAC 3 cut(s) 260, 344, 581
MalI GATC 3 cut(s) 231, 312, 552
MboI GATC 3 cut(s) 229, 310, 550
MboII GAAGA 5 cut(s) 49, 150, 194, 296, 487
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 1 cut(s) 218
MluCI AATT 4 cut(s) 302, 476, 491, 527
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 552
MseI TTAA 1 cut(s) 599
MwoI GCNNNNNNNGC 3 cut(s) 14, 200, 213
NdeII GATC 3 cut(s) 229, 310, 550
NlaIV GGNNCC 2 cut(s) 438, 451
NmeAIII GCCGAG 2 cut(s) 123, 421
NmuCI GTSAC 1 cut(s) 344
PaeR7I CTCGAG 2 cut(s) 256, 268
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 231
PfeI GAWTC 1 cut(s) 356
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
Psp124BI GAGCTC 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 438, 451
PspXI VCTCGAGB 2 cut(s) 256, 268
PsuI RGATCY 1 cut(s) 310
RsaI GTAC 1 cut(s) 148
RsaNI GTAC 1 cut(s) 147
SacI GAGCTC 1 cut(s) 218
SalI GTCGAC 1 cut(s) 236
SaqAI TTAA 1 cut(s) 599
Sau3AI GATC 3 cut(s) 229, 310, 550
SchI GAGTC 1 cut(s) 586
SduI GDGCHC 1 cut(s) 218
SfcI CTRYAG 1 cut(s) 471
Sfr274I CTCGAG 2 cut(s) 256, 268
SgrDI CGTCGACG 1 cut(s) 236
SlaI CTCGAG 2 cut(s) 256, 268
SmlI CTYRAG 2 cut(s) 256, 268
SmoI CTYRAG 2 cut(s) 256, 268
Sse9I AATT 4 cut(s) 302, 476, 491, 527
SsiI CCGC 3 cut(s) 17, 434, 503
SspMI CTAG 3 cut(s) 105, 210, 405
SstI GAGCTC 1 cut(s) 218
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 3 cut(s) 60, 163, 329
TaiI ACGT 3 cut(s) 237, 368, 583
TaqI TCGA 7 cut(s) 87, 122, 225, 237, 257, 269, 595
TasI AATT 4 cut(s) 302, 476, 491, 527
TatI WGTACW 1 cut(s) 146
TfiI GAWTC 1 cut(s) 356
Tru1I TTAA 1 cut(s) 599
Tru9I TTAA 1 cut(s) 599
TseFI GTSAC 1 cut(s) 344
Tsp45I GTSAC 1 cut(s) 344
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 3 cut(s) 302, 491, 527
XhoI CTCGAG 2 cut(s) 256, 268
XmaJI CCTAGG 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 3 cut(s) 105, 210, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.