MD00G1044500.v1.1

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009816
Physical Location & Seq
Reverse (-)
8161951 .. 8163479
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1044500.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGAGGTGTCTCGAGCTGCCACGTCAGCGACGGCGGCGAAGAAGAGGAAGGCCGGCAGCGCGGACGGAGAATCAGTCGAATTGCCGTCGTCGTATGACGTGGCTCAGCACAAGAGGCCGAGAAGGCGCGTCGTCGTGGTTAGCAGCGCGCCGAAAATCAAGAGGGAGGCGGAGAGCGTACGGACTTCCAACGACGGCTTTTGGACGTCGGATCACGCGGAGAGCTCCTGCTGCTCGAGTAACGGCTCGAGCGAGCTGGAAGATGAGAGTGACCAGGTCGAATCGTGGACGTATAATTCCAGCAGAGACGAAAGGAGAGAGATGACGGCGGCGACGAGCAAAGTTGGAGCGGAAGCAGAATCGACGGCGAGGCTTAAAGATGAGTCTCAACGACGATCACCGACGGTAGTGAACGCGTCGGAGCTCGAAGAATTCTTCGCTGCCATGGAAAAGGAAAGCCAGCAAAAATTTAAGGAGATGTACAATTTTGATGTTGCCAAGGACGAGCCGCACGAAGGACGATACGAGTGGGTTCGATTAAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

183

Amino Acids

20.41

Weight (kDa)

5.43

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 137 - 180 2.3e-14 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 209
AccBSI CCGCTC 1 cut(s) 350
AccII CGCG 5 cut(s) 62, 129, 149, 218, 416
AciI CCGC 7 cut(s) 35, 62, 170, 218, 329, 350, 509
AclWI GGATC 1 cut(s) 219
AcsI RAATTY 2 cut(s) 431, 467
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 2 cut(s) 180, 482
AfiI CCNNNNNNNGG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 414
AjiI CACGTC 2 cut(s) 24, 100
AjnI CCWGG 1 cut(s) 273
AluBI AGCT 4 cut(s) 17, 225, 256, 424
AluI AGCT 4 cut(s) 17, 225, 256, 424
Alw21I GWGCWC 2 cut(s) 227, 426
Alw26I GTCTC 3 cut(s) 15, 300, 390
AlwI GGATC 1 cut(s) 219
Ama87I CYCGRG 3 cut(s) 12, 235, 247
AoxI GGCC 2 cut(s) 51, 116
ApeKI GCWGC 5 cut(s) 17, 57, 144, 231, 440
ApoI RAATTY 2 cut(s) 431, 467
AspLEI GCGC 4 cut(s) 62, 129, 149, 151
AsuHPI GGTGA 1 cut(s) 390
AvaI CYCGRG 3 cut(s) 12, 235, 247
BanII GRGCYC 2 cut(s) 227, 426
Bbv12I GWGCWC 2 cut(s) 227, 426
BbvI GCAGC 5 cut(s) 4, 69, 156, 218, 427
BceAI ACGGC 6 cut(s) 48, 70, 211, 259, 342, 381
BciT130I CCWGG 1 cut(s) 275
BcoDI GTCTC 3 cut(s) 15, 300, 390
BglI GCCNNNNNGGC 1 cut(s) 124
BisI GCNGC 8 cut(s) 18, 36, 58, 145, 232, 330, 441, 509
BlpI GCTNAGC 1 cut(s) 105
BlsI GCNGC 8 cut(s) 19, 37, 59, 146, 233, 331, 442, 510
Bme1390I CCNGG 1 cut(s) 275
BmeT110I CYCGRG 3 cut(s) 12, 235, 247
BmgBI CACGTC 2 cut(s) 24, 100
BmrFI CCNGG 1 cut(s) 275
Bpu1102I GCTNAGC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 206
BsaJI CCNNGG 2 cut(s) 444, 498
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 1 cut(s) 515
Bse118I RCCGGY 1 cut(s) 53
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 444, 498
BseLI CCNNNNNNNGG 1 cut(s) 515
BseMII CTCAG 1 cut(s) 119
BsePI GCGCGC 1 cut(s) 147
BseXI GCAGC 5 cut(s) 4, 69, 156, 218, 427
Bsh1236I CGCG 5 cut(s) 62, 129, 149, 218, 416
BshFI GGCC 2 cut(s) 53, 118
BsiHKAI GWGCWC 2 cut(s) 227, 426
BsiHKCI CYCGRG 3 cut(s) 12, 235, 247
BsiSI CCGG 1 cut(s) 54
BsiWI CGTACG 1 cut(s) 178
BslI CCNNNNNNNGG 1 cut(s) 515
BsmAI GTCTC 3 cut(s) 15, 300, 390
BsmBI CGTCTC 1 cut(s) 300
BsnI GGCC 2 cut(s) 53, 118
BsoBI CYCGRG 3 cut(s) 12, 235, 247
Bsp1286I GDGCHC 2 cut(s) 227, 426
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 2 cut(s) 211, 395
Bsp1720I GCTNAGC 1 cut(s) 105
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 7 cut(s) 35, 62, 170, 218, 329, 350, 509
BspANI GGCC 2 cut(s) 53, 118
BspCNI CTCAG 1 cut(s) 118
BspFNI CGCG 5 cut(s) 62, 129, 149, 218, 416
BspPI GGATC 1 cut(s) 219
BsrBI CCGCTC 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 53
BsrGI TGTACA 1 cut(s) 480
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 2 cut(s) 444, 498
BssHII GCGCGC 1 cut(s) 147
BssMI GATC 2 cut(s) 211, 395
BssNI GRCGYC 1 cut(s) 206
BssT1I CCWWGG 2 cut(s) 444, 498
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 1 cut(s) 406
Bst6I CTCTTC 1 cut(s) 38
BstACI GRCGYC 1 cut(s) 206
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 4 cut(s) 55, 149, 254, 461
BstDEI CTNAG 1 cut(s) 105
BstDSI CCRYGG 1 cut(s) 444
BstFNI CGCG 5 cut(s) 62, 129, 149, 218, 416
BstHHI GCGC 4 cut(s) 62, 129, 149, 151
BstKTI GATC 2 cut(s) 214, 398
BstMAI GTCTC 3 cut(s) 15, 300, 390
BstMBI GATC 2 cut(s) 211, 395
BstMWI GCNNNNNNNGC 6 cut(s) 26, 35, 59, 115, 124, 231
BstNI CCWGG 1 cut(s) 275
BstSCI CCNGG 1 cut(s) 273
BstUI CGCG 5 cut(s) 62, 129, 149, 218, 416
BstV1I GCAGC 5 cut(s) 4, 69, 156, 218, 427
BsuRI GGCC 2 cut(s) 53, 118
BtgI CCRYGG 1 cut(s) 444
BtrI CACGTC 2 cut(s) 24, 100
Cac8I GCNNGC 4 cut(s) 55, 149, 254, 461
CfoI GCGC 4 cut(s) 62, 129, 149, 151
Cfr10I RCCGGY 1 cut(s) 53
CseI GACGC 2 cut(s) 118, 405
CsiI ACCWGGT 1 cut(s) 273
Csp6I GTAC 2 cut(s) 179, 481
CviAII CATG 2 cut(s) 445, 546
CviQI GTAC 2 cut(s) 179, 481
DdeI CTNAG 1 cut(s) 105
DpnI GATC 2 cut(s) 213, 397
DpnII GATC 2 cut(s) 211, 395
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 2 cut(s) 225, 424
Eco130I CCWWGG 2 cut(s) 444, 498
Eco24I GRGCYC 2 cut(s) 227, 426
Eco53kI GAGCTC 2 cut(s) 225, 424
Eco88I CYCGRG 3 cut(s) 12, 235, 247
EcoICRI GAGCTC 2 cut(s) 225, 424
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 273
EcoT14I CCWWGG 2 cut(s) 444, 498
EcoT38I GRGCYC 2 cut(s) 227, 426
ErhI CCWWGG 2 cut(s) 444, 498
Esp3I CGTCTC 1 cut(s) 300
FaeI CATG 2 cut(s) 448, 549
FaiI YATR 4 cut(s) 96, 294, 446, 547
FatI CATG 2 cut(s) 444, 545
Fnu4HI GCNGC 8 cut(s) 18, 36, 58, 145, 232, 330, 441, 509
FriOI GRGCYC 2 cut(s) 227, 426
Fsp4HI GCNGC 8 cut(s) 18, 36, 58, 145, 232, 330, 441, 509
GlaI GCGC 4 cut(s) 61, 128, 148, 150
GluI GCNGC 8 cut(s) 18, 36, 58, 145, 232, 330, 441, 509
HaeIII GGCC 2 cut(s) 53, 118
HapII CCGG 1 cut(s) 54
HgaI GACGC 2 cut(s) 118, 405
HhaI GCGC 4 cut(s) 62, 129, 149, 151
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 2 cut(s) 448, 549
Hin6I GCGC 4 cut(s) 60, 127, 147, 149
HinP1I GCGC 4 cut(s) 60, 127, 147, 149
HinfI GANTC 4 cut(s) 71, 281, 359, 383
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 390
Hpy166II GTNNAC 2 cut(s) 288, 412
Hpy188I TCNGA 2 cut(s) 211, 421
Hpy188III TCNNGA 2 cut(s) 12, 160
Hpy8I GTNNAC 2 cut(s) 288, 412
HpyAV CCTTC 3 cut(s) 43, 117, 509
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4IV ACGT 4 cut(s) 23, 99, 206, 290
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 35, 59, 115, 124, 231
HpyF3I CTNAG 1 cut(s) 105
HpySE526I ACGT 4 cut(s) 23, 99, 206, 290
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 2 cut(s) 448, 549
HspAI GCGC 4 cut(s) 60, 127, 147, 149
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 2 cut(s) 211, 395
LmnI GCTCC 3 cut(s) 230, 347, 421
LpnPI CCDG 7 cut(s) 67, 241, 242, 260, 287, 313, 473
Lsp1109I GCAGC 5 cut(s) 4, 69, 156, 218, 427
MabI ACCWGGT 1 cut(s) 273
MaeII ACGT 4 cut(s) 23, 99, 206, 290
MaeIII GTNAC 2 cut(s) 239, 269
MalI GATC 2 cut(s) 213, 397
MbiI CCGCTC 1 cut(s) 350
MboI GATC 2 cut(s) 211, 395
MboII GAAGA 5 cut(s) 52, 55, 272, 427, 440
MhlI GDGCHC 2 cut(s) 227, 426
MluCI AATT 5 cut(s) 80, 295, 431, 467, 484
MluI ACGCGT 1 cut(s) 414
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 4 cut(s) 189, 213, 325, 399
MnlI CCTC 5 cut(s) 39, 108, 156, 160, 363
MroNI GCCGGC 1 cut(s) 53
MseI TTAA 3 cut(s) 375, 471, 539
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 1 cut(s) 275
MvaI CCWGG 1 cut(s) 275
MvnI CGCG 5 cut(s) 62, 129, 149, 218, 416
MwoI GCNNNNNNNGC 6 cut(s) 26, 35, 59, 115, 124, 231
NaeI GCCGGC 1 cut(s) 55
NcoI CCATGG 1 cut(s) 444
NdeII GATC 2 cut(s) 211, 395
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 2 cut(s) 448, 549
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 269
PaeR7I CTCGAG 3 cut(s) 12, 235, 247
PauI GCGCGC 1 cut(s) 147
PcsI WCGNNNNNNNCGW 3 cut(s) 249, 332, 510
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 71, 281, 359
Pfl23II CGTACG 1 cut(s) 178
PflFI GACNNNGTC 1 cut(s) 275
PkrI GCNGC 8 cut(s) 19, 37, 59, 146, 233, 331, 442, 510
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Psp124BI GAGCTC 2 cut(s) 227, 426
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PspLI CGTACG 1 cut(s) 178
PspXI VCTCGAGB 2 cut(s) 235, 247
PsyI GACNNNGTC 1 cut(s) 275
PteI GCGCGC 1 cut(s) 147
RsaI GTAC 2 cut(s) 180, 482
RsaNI GTAC 2 cut(s) 179, 481
SacI GAGCTC 2 cut(s) 227, 426
SaqAI TTAA 3 cut(s) 375, 471, 539
SatI GCNGC 8 cut(s) 18, 36, 58, 145, 232, 330, 441, 509
Sau3AI GATC 2 cut(s) 211, 395
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 2 cut(s) 227, 426
SexAI ACCWGGT 1 cut(s) 273
Sfr274I CTCGAG 3 cut(s) 12, 235, 247
SlaI CTCGAG 3 cut(s) 12, 235, 247
SmlI CTYRAG 3 cut(s) 12, 235, 247
SmoI CTYRAG 3 cut(s) 12, 235, 247
Sse9I AATT 5 cut(s) 80, 295, 431, 467, 484
SsiI CCGC 7 cut(s) 35, 62, 170, 218, 329, 350, 509
SstI GAGCTC 2 cut(s) 227, 426
StyD4I CCNGG 1 cut(s) 273
StyI CCWWGG 2 cut(s) 444, 498
TaaI ACNGT 1 cut(s) 406
TaiI ACGT 4 cut(s) 26, 102, 209, 293
TaqI TCGA 8 cut(s) 13, 78, 236, 248, 279, 362, 426, 535
TasI AATT 5 cut(s) 80, 295, 431, 467, 484
TatI WGTACW 1 cut(s) 480
TauI GCSGC 3 cut(s) 38, 332, 511
TfiI GAWTC 3 cut(s) 71, 281, 359
Tru1I TTAA 3 cut(s) 375, 471, 539
Tru9I TTAA 3 cut(s) 375, 471, 539
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 5 cut(s) 17, 57, 144, 231, 440
Tsp45I GTSAC 1 cut(s) 269
TspGWI ACGGA 2 cut(s) 81, 196
Tth111I GACNNNGTC 1 cut(s) 275
XapI RAATTY 2 cut(s) 431, 467
XhoI CTCGAG 3 cut(s) 12, 235, 247
ZraI GACGTC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.