AT2G24540

Kelch

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013470
Physical Location & Seq
Forward (+)
10426342 .. 10427683
1342 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G24540.1

Sequence Viewer

Length: 1119 bp
ATGGCAGAACAAGAAACTACGTCAAACATAAACACCATTAATGATCAAGCAGAAGAAGAAACAAGAACAAAATCTCAGCCATTGATTTCAGGTCTACCAAATGATATAGCTGAGCTTTGTCTTCTTCGTCTTCCTTACCCATACCATGCTCTATATCGCTCTGTTTCATCTTCATGGAACAAAACCATCACAAACCCTAGATTCCTCTTCTCCAAGCAATCTCTTTCTATCTCTTCTCCTTACCTATTTGTCTTCGCTTTCAACAAATCAACGGCTAGGATTCAATGGCAATCCCTTGACCTTGCATCTGGCCGTTGGTTTGTTTTACCTCCCATGCCAAACTCTTTCACCAAAATCTCGTCTCCTCACGCACTCTCATGCGCGTCGATGCCACGTCAGGGGAAGCTTTTTGTACTCGGCGGCGGAGATGTAAACCGCTCCGCCGTCGTTTACACTGCTCTAACGAATCGTTGGTCGTGTATATCTCCGATGATGAGCCCGAGAACGTACTTCGTCTCCGGGAATGTTAACGGTAAAATCATGGCCGTTGGTGGGAGTGTGGGCGGAAACGGAGAAGCAACTACGGAAGTTGAATCGTATGATCCAGATAATGACACGTGGACGGTTGTGAAGAAGTTGCCGATGGTGTTGGCGAAGTATGACTCAGCCGTGATCGGGAAAGAGATGTGTGTAACAGAAGGATGGGCGTGGCCGTTCATGTTTCCACCGATGGGACAAGTGTACGACTCCGACGAAGGCACGTGGCGAGAGATGAGCGGTGGGATGAAAGAAGGGTGGACGGGTGTGAGCGTCGTGATCCGCGACAGGCTGTTTGTGATCTCGGAGCACGGTGATTTTCCGATGAAGGTTTATTGTTCAGATGATGACACGTGGAGGTACGTGAGTGGTGAGAAGCTACAGGGGGAGAAGATGCGGCGTCCTTTCGCCGTGACGGGAGCTGATGATCGTGTGTTTGTGGTGGCCAGTGGGATTAACGTAGCGGAAGGGAGAGTGAGTGAAGGGCAAAATGGGGATTTTAGTGTTGAGTGGAGGATGGTTTCGTCTCCTAAAAGTTCTATTCAGTTTTCACCTGCTAGTTGTCATGTGCTATATGTTTGA

Protein Analysis

372

Amino Acids

41.28

Weight (kDa)

5.91

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 29 - 70 3.8e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 94 - 257 3e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 104 - 218 3.5e-10 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 124 - 305 8.6e-13 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 136 - 276 7.4e-08 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 167 - 214 7.7e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24540
fragaria_vesca FvH4_2g36671
malus_domestica MD08G1127900.v1.1 MD15G1107400.v1.1
prunus_persica Prupe.1G459700_v2.0.a1 Prupe.5G016700_v2.0.a1
pyrus_communis pycom08g10690 pycom15g09820
rosa_chinensis RchiOBHm_Chr6g0305031
rosa_laevigata RLG00000010926
rosa_multiflora Rmu_sc0009145.1_g000002
rosa_roxburghii Rroxscaffold_7G00163240
rosa_rugosa Rorug06G0336900
rosa_samantha Rh6AG448500 Rh6BG452400 Rh6CG462200 Rh6DG449100
rosa_wichuraiana Rw6G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1099
Acc36I ACCTGC 1 cut(s) 1099
AccBSI CCGCTC 2 cut(s) 438, 777
AccI GTMKAC 1 cut(s) 94
AccII CGCG 2 cut(s) 383, 822
AciI CCGC 9 cut(s) 420, 423, 436, 441, 564, 777, 820, 934, 1001
AclWI GGATC 2 cut(s) 596, 811
AcoI YGGCCR 4 cut(s) 310, 543, 710, 981
AcvI CACGTG 3 cut(s) 618, 762, 891
AcyI GRCGYC 1 cut(s) 937
AfaI GTAC 4 cut(s) 414, 509, 743, 899
AfiI CCNNNNNNNGG 4 cut(s) 398, 552, 675, 731
AflIII ACRYGT 2 cut(s) 615, 888
AgsI TTSAA 3 cut(s) 262, 284, 593
AjiI CACGTC 1 cut(s) 395
AluBI AGCT 5 cut(s) 110, 115, 406, 916, 959
AluI AGCT 5 cut(s) 110, 115, 406, 916, 959
Alw21I GWGCWC 1 cut(s) 849
Alw26I GTCTC 3 cut(s) 366, 520, 1068
AlwI GGATC 2 cut(s) 596, 811
Ama87I CYCGRG 1 cut(s) 499
AoxI GGCC 4 cut(s) 310, 543, 710, 981
ArsI GACNNNNNNTTYG 4 cut(s) 458, 490, 815, 847
AseI ATTAAT 1 cut(s) 39
AspLEI GCGC 1 cut(s) 383
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 4 cut(s) 340, 863, 920, 1080
AvaI CYCGRG 1 cut(s) 499
BaeI ACNNNNGTAYC 2 cut(s) 889, 922
BalI TGGCCA 1 cut(s) 983
BanII GRGCYC 1 cut(s) 500
BbrPI CACGTG 3 cut(s) 618, 762, 891
BbsI GAAGAC 3 cut(s) 113, 122, 244
Bbv12I GWGCWC 1 cut(s) 849
BccI CCATC 5 cut(s) 194, 637, 696, 724, 1048
BceAI ACGGC 7 cut(s) 288, 297, 428, 530, 653, 697, 932
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 1 cut(s) 520
BcoDI GTCTC 3 cut(s) 366, 520, 1068
BfaI CTAG 3 cut(s) 198, 276, 1095
BfmI CTRYAG 1 cut(s) 917
BfuAI ACCTGC 1 cut(s) 1099
BisI GCNGC 2 cut(s) 421, 935
BlpI GCTNAGC 1 cut(s) 111
BlsI GCNGC 2 cut(s) 422, 936
Bme1390I CCNGG 1 cut(s) 520
BmeT110I CYCGRG 1 cut(s) 499
BmgBI CACGTC 1 cut(s) 395
BmrFI CCNGG 1 cut(s) 520
BmsI GCATC 3 cut(s) 314, 378, 921
BpiI GAAGAC 3 cut(s) 113, 122, 244
Bpu1102I GCTNAGC 1 cut(s) 111
BpuMI CCSGG 1 cut(s) 520
BsaAI YACGTR 4 cut(s) 618, 762, 891, 901
BsaHI GRCGYC 1 cut(s) 937
BsaXI ACNNNNNCTCC 4 cut(s) 500, 530, 1000, 1030
Bsc4I CCNNNNNNNGG 4 cut(s) 398, 552, 675, 731
Bse1I ACTGG 1 cut(s) 984
BseGI GGATG 3 cut(s) 707, 789, 1059
BseLI CCNNNNNNNGG 4 cut(s) 398, 552, 675, 731
BseMII CTCAG 3 cut(s) 89, 102, 678
BseNI ACTGG 1 cut(s) 984
BseRI GAGGAG 1 cut(s) 354
Bsh1236I CGCG 2 cut(s) 383, 822
BshFI GGCC 4 cut(s) 312, 545, 712, 983
BsiHKAI GWGCWC 1 cut(s) 849
BsiHKCI CYCGRG 1 cut(s) 499
BsiSI CCGG 1 cut(s) 519
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 4 cut(s) 398, 552, 675, 731
BsmAI GTCTC 3 cut(s) 366, 520, 1068
BsmBI CGTCTC 3 cut(s) 366, 520, 1068
BsmFI GGGAC 1 cut(s) 747
BsnI GGCC 4 cut(s) 312, 545, 712, 983
BsoBI CYCGRG 1 cut(s) 499
Bsp1286I GDGCHC 2 cut(s) 500, 849
Bsp143I GATC 6 cut(s) 43, 601, 672, 816, 837, 964
Bsp1720I GCTNAGC 1 cut(s) 111
BspACI CCGC 9 cut(s) 420, 423, 436, 441, 564, 777, 820, 934, 1001
BspANI GGCC 4 cut(s) 312, 545, 712, 983
BspCNI CTCAG 3 cut(s) 88, 103, 677
BspFNI CGCG 2 cut(s) 383, 822
BspMI ACCTGC 1 cut(s) 1099
BspPI GGATC 2 cut(s) 596, 811
BsrBI CCGCTC 2 cut(s) 438, 777
BsrI ACTGG 1 cut(s) 984
BssMI GATC 6 cut(s) 43, 601, 672, 816, 837, 964
BssNI GRCGYC 1 cut(s) 937
Bst4CI ACNGT 3 cut(s) 533, 625, 851
Bst6I CTCTTC 2 cut(s) 212, 238
BstACI GRCGYC 1 cut(s) 937
BstBAI YACGTR 4 cut(s) 618, 762, 891, 901
BstDEI CTNAG 3 cut(s) 75, 111, 664
BstF5I GGATG 3 cut(s) 707, 789, 1059
BstFNI CGCG 2 cut(s) 383, 822
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 6 cut(s) 46, 604, 675, 819, 840, 967
BstMAI GTCTC 3 cut(s) 366, 520, 1068
BstMBI GATC 6 cut(s) 43, 601, 672, 816, 837, 964
BstSCI CCNGG 1 cut(s) 518
BstSFI CTRYAG 1 cut(s) 917
BstUI CGCG 2 cut(s) 383, 822
BstV2I GAAGAC 3 cut(s) 113, 122, 244
BsuRI GGCC 4 cut(s) 312, 545, 712, 983
BtrI CACGTC 1 cut(s) 395
BtsCI GGATG 3 cut(s) 707, 789, 1059
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 453, 991
BveI ACCTGC 1 cut(s) 1099
CfoI GCGC 1 cut(s) 383
CseI GACGC 3 cut(s) 372, 799, 926
Csp6I GTAC 4 cut(s) 413, 508, 742, 898
CviAII CATG 7 cut(s) 146, 174, 334, 378, 541, 718, 1103
CviQI GTAC 4 cut(s) 413, 508, 742, 898
DdeI CTNAG 3 cut(s) 75, 111, 664
DpnI GATC 6 cut(s) 45, 603, 674, 818, 839, 966
DpnII GATC 6 cut(s) 43, 601, 672, 816, 837, 964
EaeI YGGCCR 4 cut(s) 310, 543, 710, 981
Eam1104I CTCTTC 2 cut(s) 212, 238
EarI CTCTTC 2 cut(s) 212, 238
EciI GGCGGA 3 cut(s) 430, 438, 579
Eco24I GRGCYC 1 cut(s) 500
Eco72I CACGTG 3 cut(s) 618, 762, 891
Eco88I CYCGRG 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 500
Esp3I CGTCTC 3 cut(s) 366, 520, 1068
FaeI CATG 7 cut(s) 149, 177, 337, 381, 544, 721, 1106
FaqI GGGAC 1 cut(s) 747
FatI CATG 7 cut(s) 145, 173, 333, 377, 540, 717, 1102
FbaI TGATCA 1 cut(s) 43
FblI GTMKAC 1 cut(s) 94
Fnu4HI GCNGC 2 cut(s) 421, 935
FokI GGATG 3 cut(s) 714, 796, 1066
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 2 cut(s) 421, 935
FspBI CTAG 3 cut(s) 198, 276, 1095
GlaI GCGC 1 cut(s) 382
GluI GCNGC 2 cut(s) 421, 935
HaeIII GGCC 4 cut(s) 312, 545, 712, 983
HapII CCGG 1 cut(s) 519
HgaI GACGC 3 cut(s) 372, 799, 926
HhaI GCGC 1 cut(s) 383
Hin1I GRCGYC 1 cut(s) 937
Hin1II CATG 7 cut(s) 149, 177, 337, 381, 544, 721, 1106
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 6 cut(s) 201, 280, 466, 593, 662, 746
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 1 cut(s) 519
HphI GGTGA 4 cut(s) 340, 863, 920, 1080
Hpy166II GTNNAC 7 cut(s) 95, 433, 451, 529, 621, 742, 798
Hpy188I TCNGA 5 cut(s) 489, 751, 844, 861, 880
Hpy188III TCNNGA 3 cut(s) 605, 676, 814
Hpy8I GTNNAC 7 cut(s) 95, 433, 451, 529, 621, 742, 798
Hpy99I CGWCG 4 cut(s) 388, 449, 755, 815
HpyAV CCTTC 6 cut(s) 692, 749, 785, 859, 998, 1013
HpyCH4III ACNGT 3 cut(s) 533, 625, 851
HpyCH4IV ACGT 8 cut(s) 20, 394, 506, 617, 761, 890, 900, 996
HpyCH4V TGCA 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 75, 111, 664
HpySE526I ACGT 8 cut(s) 20, 394, 506, 617, 761, 890, 900, 996
Hsp92I GRCGYC 1 cut(s) 937
Hsp92II CATG 7 cut(s) 149, 177, 337, 381, 544, 721, 1106
HspAI GCGC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 43
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 6 cut(s) 43, 601, 672, 816, 837, 964
LmnI GCTCC 3 cut(s) 443, 844, 956
LpnPI CCDG 9 cut(s) 75, 294, 383, 532, 618, 811, 905, 997, 1104
LweI GCATC 3 cut(s) 314, 378, 921
MaeI CTAG 3 cut(s) 198, 276, 1095
MaeII ACGT 8 cut(s) 20, 394, 506, 617, 761, 890, 900, 996
MaeIII GTNAC 2 cut(s) 691, 949
MalI GATC 6 cut(s) 45, 603, 674, 818, 839, 966
MbiI CCGCTC 2 cut(s) 438, 777
MboI GATC 6 cut(s) 43, 601, 672, 816, 837, 964
MhlI GDGCHC 2 cut(s) 500, 849
MlsI TGGCCA 1 cut(s) 983
MluNI TGGCCA 1 cut(s) 983
MlyI GAGTC 2 cut(s) 656, 740
MmeI TCCRAC 1 cut(s) 774
MnlI CCTC 5 cut(s) 215, 339, 375, 888, 1044
Mox20I TGGCCA 1 cut(s) 983
MscI TGGCCA 1 cut(s) 983
MseI TTAA 3 cut(s) 39, 528, 993
MslI CAYNNNNRTG 2 cut(s) 172, 376
Msp20I TGGCCA 1 cut(s) 983
MspI CCGG 1 cut(s) 519
MspR9I CCNGG 1 cut(s) 520
MvnI CGCG 2 cut(s) 383, 822
NciI CCSGG 1 cut(s) 520
NdeII GATC 6 cut(s) 43, 601, 672, 816, 837, 964
NlaIII CATG 7 cut(s) 149, 177, 337, 381, 544, 721, 1106
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 1 cut(s) 949
PaqCI CACCTGC 1 cut(s) 1099
PcsI WCGNNNNNNNCGW 2 cut(s) 750, 819
PfeI GAWTC 4 cut(s) 201, 280, 466, 593
PfoI TCCNGGA 1 cut(s) 518
PkrI GCNGC 2 cut(s) 422, 936
PleI GAGTC 2 cut(s) 656, 740
PmaCI CACGTG 3 cut(s) 618, 762, 891
PmlI CACGTG 3 cut(s) 618, 762, 891
PpsI GAGTC 2 cut(s) 656, 740
Ppu21I YACGTR 4 cut(s) 618, 762, 891, 901
PshBI ATTAAT 1 cut(s) 39
PspCI CACGTG 3 cut(s) 618, 762, 891
RsaI GTAC 4 cut(s) 414, 509, 743, 899
RsaNI GTAC 4 cut(s) 413, 508, 742, 898
RseI CAYNNNNRTG 2 cut(s) 172, 376
SaqAI TTAA 3 cut(s) 39, 528, 993
SatI GCNGC 2 cut(s) 421, 935
Sau3AI GATC 6 cut(s) 43, 601, 672, 816, 837, 964
SchI GAGTC 2 cut(s) 656, 740
ScrFI CCNGG 1 cut(s) 520
SduI GDGCHC 2 cut(s) 500, 849
SfaNI GCATC 3 cut(s) 314, 378, 921
SfcI CTRYAG 1 cut(s) 917
SmiMI CAYNNNNRTG 2 cut(s) 172, 376
SsiI CCGC 9 cut(s) 420, 423, 436, 441, 564, 777, 820, 934, 1001
SspMI CTAG 3 cut(s) 198, 276, 1095
StyD4I CCNGG 1 cut(s) 518
TaaI ACNGT 3 cut(s) 533, 625, 851
TaiI ACGT 8 cut(s) 23, 397, 509, 620, 764, 893, 903, 999
TaqI TCGA 1 cut(s) 386
TatI WGTACW 1 cut(s) 412
TauI GCSGC 2 cut(s) 423, 937
TfiI GAWTC 4 cut(s) 201, 280, 466, 593
Tru1I TTAA 3 cut(s) 39, 528, 993
Tru9I TTAA 3 cut(s) 39, 528, 993
TscAI CASTG 2 cut(s) 460, 991
TseFI GTSAC 1 cut(s) 949
Tsp45I GTSAC 1 cut(s) 949
TspDTI ATGAA 5 cut(s) 156, 162, 706, 800, 878
TspGWI ACGGA 2 cut(s) 585, 599
TspRI CASTG 2 cut(s) 460, 991
VspI ATTAAT 1 cut(s) 39
XmiI GTMKAC 1 cut(s) 94
XspI CTAG 3 cut(s) 198, 276, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.