Rw6G039060

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013470
Physical Location & Seq
Forward (+)
63097472 .. 63099801
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G039060.1

Sequence Viewer

Length: 1137 bp
ATGGCAGCTCTGACTTCCTCTGCATCAATCCCAGAAACTGAGAATTCTGACGTAGAAAGGAGCAGAAGCAACAAATCTGAGCCGTTGATTCCGGGACTTCCAGACGAGGTGGCCGAGCTGTGTCTTGTTTACCTTCCGTATCCGTACCAAGCTTTGGTGCGTTCGGTCTCTGCTTCGTGGAGGAGAGCCATCTCAGACCCGGCTTTTCTTCACTGCAAGAAGTCACTTTCGCTCCCGCTGCCCTATCTTTTCGTTTTCGCCTTCAACAAATTGACGGCCAGGATTCAGTGGCAAGCGCTCGACCCTAGATCGGGCCGTTGGTTTGTCCTACCTCCGATGCCGGGGCATTATTCCAAGCTCGCGAGTCCACCGGGGTTAGCGTGTGCCTCCTTGCCACGTCAGGGGATGCTTGTCGTGTTGGGTGGATCGGACGCGGAATCGTCTATGCGGTCCACTATTGTATACCGTACATCTACCAATCAGTGGTCCAGCATGACTCCCATGCCGACCGCCCGATCGTATTTTGACGCGGGAAGCATCAATAATAAGATCATAGCAGTCGGAGGTAGTGGGACCCGCGAGAATGACTCGATCAGGGCGGTTGAGTGTTATAACCCTGAAAATGACACATGGGCGACACTGGCCACGATGCCAGCTGGACTTGCCAAGTATGACTCCCACGTGGTGGGGGATAAGATGTACGTCACCGAGGGGTGGACGTGGCCCTTCATGTTCTCGCCACGCGGCCTGATCTACGACTCGAAGACGGACGCGTGGCGAGAGATGAGTCCTGGGATGAGGGAAGGGTGGACAGGTGTCAGCGTGGTCGTGGGCAATAAGTTATTAGTGATCTCGGAGTATGGAGATTGTCCGATGAAGGTTTATGACCACGATAAGGACACATGGCGGTACGTCGGCGGGGACAAGTTCCCGTGCGACTCACTTAGACGGCCTTTCGCTGTGAGCGCGGTGGAGGGGAGCATATATGTGGTGGCATGTGGGCTGAATGTGGGAATAGGAAGGCTGTCTGAATCAGAAGCAGAAAAGGGTGAGCTACAAGTTGAGTGGCAGGTTCTGCCAGCTCCAAGTGCTTTTAGGGGTTTTTCTCCTTCTAGTTGTGAAGTGCTTTATGCCTGA

Protein Analysis

378

Amino Acids

41.59

Weight (kDa)

5.92

Isoelectric Point (pI)

55.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 127 - 265 5.3e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 128 - 289 1.4e-08 FBX42, beta-propeller domain
Kelch_1 PF01344 134 - 169 4.3e-06 Kelch motif
Beta-prop_Calicin PF13964 137 - 264 2.3e-12 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 139 - 307 1.3e-16 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 139 - 276 1e-08 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 171 - 218 1.1e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24540
fragaria_vesca FvH4_2g36671
malus_domestica MD08G1127900.v1.1 MD15G1107400.v1.1
prunus_persica Prupe.1G459700_v2.0.a1 Prupe.5G016700_v2.0.a1
pyrus_communis pycom08g10690 pycom15g09820
rosa_chinensis RchiOBHm_Chr6g0305031
rosa_laevigata RLG00000010926
rosa_multiflora Rmu_sc0009145.1_g000002
rosa_roxburghii Rroxscaffold_7G00163240
rosa_rugosa Rorug06G0336900
rosa_samantha Rh6AG448500 Rh6BG452400 Rh6CG462200 Rh6DG449100
rosa_wichuraiana Rw6G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
Acc36I ACCTGC 1 cut(s) 1060
AccB7I CCANNNNNTGG 3 cut(s) 154, 483, 685
AccI GTMKAC 1 cut(s) 462
AccII CGCG 7 cut(s) 362, 434, 530, 579, 744, 773, 968
AclWI GGATC 1 cut(s) 433
AcoI YGGCCR 3 cut(s) 111, 276, 642
AcsI RAATTY 1 cut(s) 43
AcvI CACGTG 1 cut(s) 682
AdeI CACNNNGTG 1 cut(s) 685
AfaI GTAC 4 cut(s) 146, 469, 701, 911
AfeI AGCGCT 1 cut(s) 297
AfiI CCNNNNNNNGG 9 cut(s) 154, 310, 311, 341, 401, 483, 685, 714, 895
AflIII ACRYGT 1 cut(s) 771
AgsI TTSAA 1 cut(s) 265
AjiI CACGTC 2 cut(s) 398, 720
AjnI CCWGG 2 cut(s) 278, 790
AluBI AGCT 7 cut(s) 8, 118, 152, 358, 656, 1054, 1082
AluI AGCT 7 cut(s) 8, 118, 152, 358, 656, 1054, 1082
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 1 cut(s) 433
AlwNI CAGNNNCTG 2 cut(s) 38, 1075
Aor51HI AGCGCT 1 cut(s) 297
AoxI GGCC 7 cut(s) 111, 276, 313, 642, 722, 745, 950
ApeKI GCWGC 2 cut(s) 5, 238
ApoI RAATTY 1 cut(s) 43
AspLEI GCGC 2 cut(s) 298, 968
AspS9I GGNCC 5 cut(s) 313, 450, 486, 573, 723
AsuC2I CCSGG 4 cut(s) 93, 200, 342, 372
AsuHPI GGTGA 2 cut(s) 697, 1061
AvaII GGWCC 3 cut(s) 450, 486, 573
BalI TGGCCA 1 cut(s) 644
BbrPI CACGTG 1 cut(s) 682
BbsI GAAGAC 1 cut(s) 770
BbvI GCAGC 2 cut(s) 17, 225
BccI CCATC 1 cut(s) 197
BceAI ACGGC 4 cut(s) 67, 291, 300, 965
BciT130I CCWGG 2 cut(s) 280, 792
BciVI GTATCC 1 cut(s) 150
BcnI CCSGG 4 cut(s) 93, 200, 342, 372
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 2 cut(s) 306, 1113
BfoI RGCGCY 1 cut(s) 299
BfuAI ACCTGC 1 cut(s) 1060
BfuI GTATCC 1 cut(s) 150
BisI GCNGC 3 cut(s) 6, 239, 745
BlsI GCNGC 3 cut(s) 7, 240, 746
Bme1390I CCNGG 6 cut(s) 93, 200, 280, 342, 372, 792
Bme18I GGWCC 3 cut(s) 450, 486, 573
BmgBI CACGTC 2 cut(s) 398, 720
BmgT120I GGNCC 5 cut(s) 313, 450, 486, 573, 723
BmiI GGNNCC 2 cut(s) 574, 575
BmrFI CCNGG 6 cut(s) 93, 200, 280, 342, 372, 792
BmsI GCATC 5 cut(s) 32, 327, 396, 546, 639
BoxI GACNNNNGTC 1 cut(s) 815
BpiI GAAGAC 1 cut(s) 770
BplI GAGNNNNNCTC 2 cut(s) 572, 604
BpuMI CCSGG 4 cut(s) 93, 200, 342, 372
BsaAI YACGTR 1 cut(s) 682
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 4 cut(s) 341, 371, 708, 791
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 9 cut(s) 154, 310, 311, 341, 401, 483, 685, 714, 895
Bse1I ACTGG 1 cut(s) 645
BseBI CCWGG 2 cut(s) 280, 792
BseDI CCNNGG 4 cut(s) 341, 371, 708, 791
BseGI GGATG 2 cut(s) 411, 801
BseLI CCNNNNNNNGG 9 cut(s) 154, 310, 311, 341, 401, 483, 685, 714, 895
BseMII CTCAG 3 cut(s) 30, 69, 207
BseNI ACTGG 1 cut(s) 645
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 2 cut(s) 17, 225
Bsh1236I CGCG 7 cut(s) 362, 434, 530, 579, 744, 773, 968
Bsh1285I CGRYCG 2 cut(s) 510, 518
BshFI GGCC 7 cut(s) 113, 278, 315, 644, 724, 747, 952
BsiEI CGRYCG 2 cut(s) 510, 518
BsiSI CCGG 4 cut(s) 92, 200, 341, 371
BslFI GGGAC 3 cut(s) 108, 586, 935
BslI CCNNNNNNNGG 9 cut(s) 154, 310, 311, 341, 401, 483, 685, 714, 895
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 3 cut(s) 108, 586, 935
BsnI GGCC 7 cut(s) 113, 278, 315, 644, 724, 747, 952
Bso31I GGTCTC 1 cut(s) 172
Bsp143I GATC 7 cut(s) 308, 425, 515, 549, 591, 750, 849
Bsp68I TCGCGA 1 cut(s) 362
BspANI GGCC 7 cut(s) 113, 278, 315, 644, 724, 747, 952
BspCNI CTCAG 3 cut(s) 31, 70, 206
BspFNI CGCG 7 cut(s) 362, 434, 530, 579, 744, 773, 968
BspLI GGNNCC 2 cut(s) 574, 575
BspMI ACCTGC 1 cut(s) 1060
BspPI GGATC 1 cut(s) 433
BspTNI GGTCTC 1 cut(s) 172
BsrI ACTGG 1 cut(s) 645
BssECI CCNNGG 4 cut(s) 341, 371, 708, 791
BssMI GATC 7 cut(s) 308, 425, 515, 549, 591, 750, 849
BssNAI GTATAC 1 cut(s) 463
Bst1107I GTATAC 1 cut(s) 463
Bst2UI CCWGG 2 cut(s) 280, 792
Bst4CI ACNGT 1 cut(s) 467
BstAPI GCANNNNNTGC 1 cut(s) 1075
BstBAI YACGTR 1 cut(s) 682
BstC8I GCNNGC 4 cut(s) 294, 360, 654, 1080
BstDEI CTNAG 4 cut(s) 39, 78, 193, 944
BstF5I GGATG 2 cut(s) 411, 801
BstFNI CGCG 7 cut(s) 362, 434, 530, 579, 744, 773, 968
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 2 cut(s) 298, 968
BstKTI GATC 7 cut(s) 311, 428, 518, 552, 594, 753, 852
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 7 cut(s) 308, 425, 515, 549, 591, 750, 849
BstMCI CGRYCG 2 cut(s) 510, 518
BstMWI GCNNNNNNNGC 6 cut(s) 238, 641, 662, 965, 1075, 1088
BstNI CCWGG 2 cut(s) 280, 792
BstNSI RCATGY 1 cut(s) 999
BstPAI GACNNNNGTC 1 cut(s) 815
BstSCI CCNGG 6 cut(s) 91, 198, 278, 340, 370, 790
BstUI CGCG 7 cut(s) 362, 434, 530, 579, 744, 773, 968
BstV1I GCAGC 2 cut(s) 17, 225
BstV2I GAAGAC 1 cut(s) 770
BstZ17I GTATAC 1 cut(s) 463
BsuI GTATCC 1 cut(s) 150
BsuRI GGCC 7 cut(s) 113, 278, 315, 644, 724, 747, 952
BtrI CACGTC 2 cut(s) 398, 720
BtsCI GGATG 2 cut(s) 411, 801
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 4 cut(s) 211, 293, 488, 638
BtuMI TCGCGA 1 cut(s) 362
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 4 cut(s) 294, 360, 654, 1080
CaiI CAGNNNCTG 2 cut(s) 38, 1075
CfoI GCGC 2 cut(s) 298, 968
Cfr13I GGNCC 5 cut(s) 313, 450, 486, 573, 723
CseI GACGC 3 cut(s) 440, 536, 779
Csp6I GTAC 4 cut(s) 145, 468, 700, 910
CspCI CAANNNNNGTGG 2 cut(s) 1046, 1081
CviAII CATG 6 cut(s) 493, 502, 630, 730, 903, 996
CviQI GTAC 4 cut(s) 145, 468, 700, 910
DdeI CTNAG 4 cut(s) 39, 78, 193, 944
DpnI GATC 7 cut(s) 310, 427, 517, 551, 593, 752, 851
DpnII GATC 7 cut(s) 308, 425, 515, 549, 591, 750, 849
DraIII CACNNNGTG 1 cut(s) 685
EaeI YGGCCR 3 cut(s) 111, 276, 642
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 3 cut(s) 450, 486, 573
Eco47III AGCGCT 1 cut(s) 297
Eco72I CACGTG 1 cut(s) 682
EcoO109I RGGNCCY 1 cut(s) 573
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 278, 790
FaeI CATG 6 cut(s) 496, 505, 633, 733, 906, 999
FaqI GGGAC 3 cut(s) 108, 586, 935
FatI CATG 6 cut(s) 492, 501, 629, 729, 902, 995
FauI CCCGC 4 cut(s) 243, 523, 584, 911
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 3 cut(s) 6, 239, 745
FokI GGATG 2 cut(s) 418, 808
Fsp4HI GCNGC 3 cut(s) 6, 239, 745
FspBI CTAG 2 cut(s) 306, 1113
GlaI GCGC 2 cut(s) 297, 967
GluI GCNGC 3 cut(s) 6, 239, 745
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 7 cut(s) 113, 278, 315, 644, 724, 747, 952
HapII CCGG 4 cut(s) 92, 200, 341, 371
HgaI GACGC 3 cut(s) 440, 536, 779
HhaI GCGC 2 cut(s) 298, 968
Hin1II CATG 6 cut(s) 496, 505, 633, 733, 906, 999
Hin6I GCGC 2 cut(s) 296, 966
HinP1I GCGC 2 cut(s) 296, 966
HindIII AAGCTT 1 cut(s) 150
HpaII CCGG 4 cut(s) 92, 200, 341, 371
HphI GGTGA 2 cut(s) 697, 1061
Hpy166II GTNNAC 6 cut(s) 130, 368, 453, 463, 717, 810
Hpy188III TCNNGA 2 cut(s) 101, 361
Hpy8I GTNNAC 6 cut(s) 130, 368, 453, 463, 717, 810
Hpy99I CGWCG 1 cut(s) 917
HpyAV CCTTC 7 cut(s) 143, 271, 736, 797, 871, 1014, 1119
HpyCH4III ACNGT 1 cut(s) 467
HpyCH4IV ACGT 6 cut(s) 51, 397, 681, 702, 719, 912
HpyCH4V TGCA 2 cut(s) 23, 216
HpyF10VI GCNNNNNNNGC 6 cut(s) 238, 641, 662, 965, 1075, 1088
HpyF3I CTNAG 4 cut(s) 39, 78, 193, 944
HpySE526I ACGT 6 cut(s) 51, 397, 681, 702, 719, 912
Hsp92II CATG 6 cut(s) 496, 505, 633, 733, 906, 999
HspAI GCGC 2 cut(s) 296, 966
KflI GGGWCCC 1 cut(s) 573
Kzo9I GATC 7 cut(s) 308, 425, 515, 549, 591, 750, 849
LmnI GCTCC 4 cut(s) 60, 237, 978, 1087
Lsp1109I GCAGC 2 cut(s) 17, 225
LweI GCATC 5 cut(s) 32, 327, 396, 546, 639
MaeI CTAG 2 cut(s) 306, 1113
MaeII ACGT 6 cut(s) 51, 397, 681, 702, 719, 912
MaeIII GTNAC 2 cut(s) 222, 703
MalI GATC 7 cut(s) 310, 427, 517, 551, 593, 752, 851
MboI GATC 7 cut(s) 308, 425, 515, 549, 591, 750, 849
MboII GAAGA 2 cut(s) 200, 775
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 2 cut(s) 43, 269
MluI ACGCGT 1 cut(s) 771
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 7 cut(s) 373, 490, 581, 668, 752, 796, 932
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 9 cut(s) 28, 100, 174, 342, 397, 557, 703, 792, 967
Mox20I TGGCCA 1 cut(s) 644
MscI TGGCCA 1 cut(s) 644
MslI CAYNNNNRTG 1 cut(s) 986
Msp20I TGGCCA 1 cut(s) 644
MspA1I CMGCKG 2 cut(s) 238, 656
MspI CCGG 4 cut(s) 92, 200, 341, 371
MspR9I CCNGG 6 cut(s) 93, 200, 280, 342, 372, 792
MvaI CCWGG 2 cut(s) 280, 792
MvnI CGCG 7 cut(s) 362, 434, 530, 579, 744, 773, 968
MwoI GCNNNNNNNGC 6 cut(s) 238, 641, 662, 965, 1075, 1088
NciI CCSGG 4 cut(s) 93, 200, 342, 372
NdeII GATC 7 cut(s) 308, 425, 515, 549, 591, 750, 849
NlaIII CATG 6 cut(s) 496, 505, 633, 733, 906, 999
NlaIV GGNNCC 2 cut(s) 574, 575
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 2 cut(s) 222, 703
NruI TCGCGA 1 cut(s) 362
NspI RCATGY 1 cut(s) 999
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PfeI GAWTC 4 cut(s) 88, 283, 437, 1031
PflMI CCANNNNNTGG 3 cut(s) 154, 483, 685
PfoI TCCNGGA 1 cut(s) 91
PkrI GCNGC 3 cut(s) 7, 240, 746
Ple19I CGATCG 1 cut(s) 518
PleI GAGTC 7 cut(s) 372, 490, 581, 668, 752, 795, 932
PmaCI CACGTG 1 cut(s) 682
PmlI CACGTG 1 cut(s) 682
PpsI GAGTC 7 cut(s) 372, 490, 581, 668, 752, 795, 932
Ppu21I YACGTR 1 cut(s) 682
PpuMI RGGWCCY 1 cut(s) 573
PshAI GACNNNNGTC 1 cut(s) 815
PsiI TTATAA 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 573
Psp6I CCWGG 2 cut(s) 278, 790
PspCI CACGTG 1 cut(s) 682
PspGI CCWGG 2 cut(s) 278, 790
PspN4I GGNNCC 2 cut(s) 574, 575
PspPI GGNCC 5 cut(s) 313, 450, 486, 573, 723
PspPPI RGGWCCY 1 cut(s) 573
PstNI CAGNNNCTG 2 cut(s) 38, 1075
PvuI CGATCG 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 656
RruI TCGCGA 1 cut(s) 362
RsaI GTAC 4 cut(s) 146, 469, 701, 911
RsaNI GTAC 4 cut(s) 145, 468, 700, 910
RseI CAYNNNNRTG 1 cut(s) 986
SatI GCNGC 3 cut(s) 6, 239, 745
Sau3AI GATC 7 cut(s) 308, 425, 515, 549, 591, 750, 849
Sau96I GGNCC 5 cut(s) 313, 450, 486, 573, 723
SchI GAGTC 7 cut(s) 373, 490, 581, 668, 752, 796, 932
ScrFI CCNGG 6 cut(s) 93, 200, 280, 342, 372, 792
SfaNI GCATC 5 cut(s) 32, 327, 396, 546, 639
SinI GGWCC 3 cut(s) 450, 486, 573
SmiMI CAYNNNNRTG 1 cut(s) 986
Sse9I AATT 2 cut(s) 43, 269
SspMI CTAG 2 cut(s) 306, 1113
StyD4I CCNGG 6 cut(s) 91, 198, 278, 340, 370, 790
TaaI ACNGT 1 cut(s) 467
TaiI ACGT 6 cut(s) 54, 400, 684, 705, 722, 915
TaqI TCGA 3 cut(s) 300, 590, 761
TaqII GACCGA 1 cut(s) 154
TasI AATT 2 cut(s) 43, 269
TauI GCSGC 1 cut(s) 747
TfiI GAWTC 4 cut(s) 88, 283, 437, 1031
TscAI CASTG 4 cut(s) 218, 293, 488, 645
TseFI GTSAC 2 cut(s) 222, 703
TseI GCWGC 2 cut(s) 5, 238
Tsp45I GTSAC 2 cut(s) 222, 703
TspDTI ATGAA 2 cut(s) 718, 890
TspGWI ACGGA 3 cut(s) 126, 132, 782
TspRI CASTG 4 cut(s) 218, 293, 488, 645
Van91I CCANNNNNTGG 3 cut(s) 154, 483, 685
VpaK11BI GGWCC 3 cut(s) 450, 486, 573
XapI RAATTY 1 cut(s) 43
XceI RCATGY 1 cut(s) 999
XmiI GTMKAC 1 cut(s) 462
XspI CTAG 2 cut(s) 306, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.