RLG00000010926

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013470
Physical Location & Seq
Reverse (-)
5456728 .. 5457876
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010926

Sequence Viewer

Length: 1149 bp
ATGGCAGCTCTGACTTCCTCTGCATCAAACCCAGAAACTGAGAATTCTGACGTAGAAAGGAGCAGAAGCAGCAGCAGCAGCAACAAATCTGAGCCGTTGATTCCTGGACTTCCAGACGAAGTGGCCGAGCTGTGTCTTCTTTACCTTCCGTATCCGTACCAAGCTTTGGTGCGCTCGGTCTCTGCTTCGTGGAGGAGAGCCATTTCAGACCCGGCTTTTCTTCACTGCAAGAAGTCACTTTCGCTCCCGCTGCCTTATCTTTTCGTTTTCGCCTTCAACAAATCGACGGCCAGGATTCAATGGCAAGCGCTCGACCCTCCATCGGGCCGTTGGTTTGTCCTACCTCCGATGCCGGGGCATTATTCCAAGCTCGCGAGTCCACCGGGGTTAGCGTGTGCCTCCTTGCCACGTCAGGGGATGCTTGTCGTGTTGGGTGGATCGGACGCGGATTCGTCTATGCGGTCCACTATTGTATACCGCACATCTACCAATCAGTGGTCCAGCATGGCTCCCATGCCGACCGCCCGATCGTATTTTGACGCGGGAAGCATCGATAATAAGATCATAGCGGTCGGAGGTAGTGGAACCCGGGAGAATGACTCGATCAAGGCCGTTGAGTGTTATAACCCTGAAAATGACACATGGGCGACACTGGCCACGATGCCAGCGGGACTTGCCAAGTATGACTCCCACGTGGTGGGGGATAAGATGTACGTCACCGAGGGGTGGACGTGGCCCTTCATGTTCTCGCCACGCGGCCTGGTCTACGACTCAAAGAGTGACGCGTGGCGAGAGATGAGTCCTGCGATGAGGGAAGGGTGGACAGGTGTCAGCGTGGTCGTGGGCAACAAGTTGTTAGTTATCTCGGAGTATGGAGATTGTCCGATGAAGGTATACGACCACGATAAGGACACATGGCGGTACGTTGGCGGGGACAAGTTCCCGTGCGACTCACTTAGACGGCCTTTCGCTGTGAGCGTGGTGGAGGGGAGCATATATGTGGTGGCATATGGGCTGAATGTGGGAATAGGAAGGCTGTCTGAATCAGAAGCAGAAAAGGGTGAGCTACAAGTTGAGTGGCAGGTTCTGCCAGCTCCAAGTGCTTTTAGGGGTTTTTCTCCTTCTAGTTGTCAAGTCCTTTATGCCTGA

Protein Analysis

383

Amino Acids

41.87

Weight (kDa)

5.71

Isoelectric Point (pI)

56.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 35 - 74 8.3e-06 F-box domain
Beta-prop_FBX42 PF13415 132 - 294 2.8e-08 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 132 - 270 6.5e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 138 - 173 3.7e-06 Kelch motif
Beta-prop_Calicin PF13964 141 - 286 3.1e-12 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 142 - 281 3.4e-08 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 143 - 311 1.5e-17 FKB95, Kelch-repeats domain
Kelch_1 PF01344 175 - 222 1.2e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24540
fragaria_vesca FvH4_2g36671
malus_domestica MD08G1127900.v1.1 MD15G1107400.v1.1
prunus_persica Prupe.1G459700_v2.0.a1 Prupe.5G016700_v2.0.a1
pyrus_communis pycom08g10690 pycom15g09820
rosa_chinensis RchiOBHm_Chr6g0305031
rosa_laevigata RLG00000010926
rosa_multiflora Rmu_sc0009145.1_g000002
rosa_roxburghii Rroxscaffold_7G00163240
rosa_rugosa Rorug06G0336900
rosa_samantha Rh6AG448500 Rh6BG452400 Rh6CG462200 Rh6DG449100
rosa_wichuraiana Rw6G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
Acc36I ACCTGC 1 cut(s) 1072
AccB7I CCANNNNNTGG 3 cut(s) 166, 495, 697
AccI GTMKAC 3 cut(s) 474, 765, 894
AccII CGCG 5 cut(s) 374, 446, 542, 756, 785
AclWI GGATC 1 cut(s) 445
AcoI YGGCCR 3 cut(s) 123, 288, 654
AcsI RAATTY 1 cut(s) 43
AcvI CACGTG 1 cut(s) 694
AdeI CACNNNGTG 1 cut(s) 697
AfaI GTAC 3 cut(s) 158, 713, 923
AfeI AGCGCT 1 cut(s) 309
AfiI CCNNNNNNNGG 9 cut(s) 166, 322, 323, 353, 413, 495, 697, 726, 907
AflIII ACRYGT 1 cut(s) 783
AgsI TTSAA 2 cut(s) 277, 299
AjiI CACGTC 2 cut(s) 410, 732
AjnI CCWGG 3 cut(s) 103, 290, 759
AluBI AGCT 6 cut(s) 8, 130, 164, 370, 1066, 1094
AluI AGCT 6 cut(s) 8, 130, 164, 370, 1066, 1094
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 1 cut(s) 445
AlwNI CAGNNNCTG 2 cut(s) 38, 1087
Ama87I CYCGRG 1 cut(s) 588
Aor51HI AGCGCT 1 cut(s) 309
AoxI GGCC 8 cut(s) 123, 288, 325, 609, 654, 734, 757, 962
ApeKI GCWGC 6 cut(s) 5, 69, 72, 75, 78, 250
ApoI RAATTY 1 cut(s) 43
AspLEI GCGC 2 cut(s) 174, 310
AspS9I GGNCC 4 cut(s) 325, 462, 498, 735
AsuC2I CCSGG 5 cut(s) 212, 354, 384, 589, 590
AsuHPI GGTGA 2 cut(s) 709, 1073
AvaI CYCGRG 1 cut(s) 588
AvaII GGWCC 2 cut(s) 462, 498
BalI TGGCCA 1 cut(s) 656
BbrPI CACGTG 1 cut(s) 694
BbsI GAAGAC 1 cut(s) 128
BbvI GCAGC 6 cut(s) 17, 81, 84, 87, 90, 237
BccI CCATC 1 cut(s) 328
BceAI ACGGC 5 cut(s) 79, 303, 312, 596, 977
BciT130I CCWGG 3 cut(s) 105, 292, 761
BciVI GTATCC 1 cut(s) 162
BcnI CCSGG 5 cut(s) 212, 354, 384, 589, 590
BcoDI GTCTC 1 cut(s) 184
BfaI CTAG 1 cut(s) 1125
BfoI RGCGCY 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 1072
BfuI GTATCC 1 cut(s) 162
BisI GCNGC 7 cut(s) 6, 70, 73, 76, 79, 251, 757
BlsI GCNGC 7 cut(s) 7, 71, 74, 77, 80, 252, 758
Bme1390I CCNGG 8 cut(s) 105, 212, 292, 354, 384, 589, 590, 761
Bme18I GGWCC 2 cut(s) 462, 498
BmeT110I CYCGRG 1 cut(s) 588
BmgBI CACGTC 2 cut(s) 410, 732
BmgT120I GGNCC 4 cut(s) 325, 462, 498, 735
BmiI GGNNCC 2 cut(s) 510, 586
BmrFI CCNGG 8 cut(s) 105, 212, 292, 354, 384, 589, 590, 761
BmsI GCATC 5 cut(s) 32, 339, 408, 558, 651
BoxI GACNNNNGTC 1 cut(s) 827
BpiI GAAGAC 1 cut(s) 128
BplI GAGNNNNNCTC 2 cut(s) 584, 616
BpuMI CCSGG 5 cut(s) 212, 354, 384, 589, 590
Bsa29I ATCGAT 1 cut(s) 552
BsaAI YACGTR 1 cut(s) 694
BsaI GGTCTC 1 cut(s) 184
BsaJI CCNNGG 4 cut(s) 353, 383, 588, 720
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bsc4I CCNNNNNNNGG 9 cut(s) 166, 322, 323, 353, 413, 495, 697, 726, 907
Bse1I ACTGG 1 cut(s) 657
BseBI CCWGG 3 cut(s) 105, 292, 761
BseCI ATCGAT 1 cut(s) 552
BseDI CCNNGG 4 cut(s) 353, 383, 588, 720
BseGI GGATG 1 cut(s) 423
BseLI CCNNNNNNNGG 9 cut(s) 166, 322, 323, 353, 413, 495, 697, 726, 907
BseMII CTCAG 2 cut(s) 30, 81
BseNI ACTGG 1 cut(s) 657
BseRI GAGGAG 1 cut(s) 208
BseXI GCAGC 6 cut(s) 17, 81, 84, 87, 90, 237
Bsh1236I CGCG 5 cut(s) 374, 446, 542, 756, 785
Bsh1285I CGRYCG 3 cut(s) 522, 530, 573
BshFI GGCC 8 cut(s) 125, 290, 327, 611, 656, 736, 759, 964
BshVI ATCGAT 1 cut(s) 552
BsiEI CGRYCG 3 cut(s) 522, 530, 573
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 4 cut(s) 212, 353, 383, 589
BslFI GGGAC 2 cut(s) 684, 947
BslI CCNNNNNNNGG 9 cut(s) 166, 322, 323, 353, 413, 495, 697, 726, 907
BsmAI GTCTC 1 cut(s) 184
BsmFI GGGAC 2 cut(s) 684, 947
BsnI GGCC 8 cut(s) 125, 290, 327, 611, 656, 736, 759, 964
Bso31I GGTCTC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 588
Bsp143I GATC 4 cut(s) 437, 527, 561, 603
Bsp68I TCGCGA 1 cut(s) 374
BspANI GGCC 8 cut(s) 125, 290, 327, 611, 656, 736, 759, 964
BspCNI CTCAG 2 cut(s) 31, 82
BspDI ATCGAT 1 cut(s) 552
BspFNI CGCG 5 cut(s) 374, 446, 542, 756, 785
BspLI GGNNCC 2 cut(s) 510, 586
BspMI ACCTGC 1 cut(s) 1072
BspPI GGATC 1 cut(s) 445
BspTNI GGTCTC 1 cut(s) 184
BsrI ACTGG 1 cut(s) 657
BssECI CCNNGG 4 cut(s) 353, 383, 588, 720
BssMI GATC 4 cut(s) 437, 527, 561, 603
BssNAI GTATAC 2 cut(s) 475, 895
Bst1107I GTATAC 2 cut(s) 475, 895
Bst2UI CCWGG 3 cut(s) 105, 292, 761
BstAPI GCANNNNNTGC 1 cut(s) 1087
BstBAI YACGTR 1 cut(s) 694
BstC8I GCNNGC 4 cut(s) 306, 372, 666, 1092
BstDEI CTNAG 3 cut(s) 39, 90, 956
BstF5I GGATG 1 cut(s) 423
BstFNI CGCG 5 cut(s) 374, 446, 542, 756, 785
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 2 cut(s) 174, 310
BstKTI GATC 4 cut(s) 440, 530, 564, 606
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 4 cut(s) 437, 527, 561, 603
BstMCI CGRYCG 3 cut(s) 522, 530, 573
BstMWI GCNNNNNNNGC 8 cut(s) 69, 75, 78, 250, 653, 674, 1087, 1100
BstNI CCWGG 3 cut(s) 105, 292, 761
BstPAI GACNNNNGTC 1 cut(s) 827
BstSCI CCNGG 8 cut(s) 103, 210, 290, 352, 382, 587, 588, 759
BstUI CGCG 5 cut(s) 374, 446, 542, 756, 785
BstV1I GCAGC 6 cut(s) 17, 81, 84, 87, 90, 237
BstV2I GAAGAC 1 cut(s) 128
BstZ17I GTATAC 2 cut(s) 475, 895
Bsu15I ATCGAT 1 cut(s) 552
BsuI GTATCC 1 cut(s) 162
BsuRI GGCC 8 cut(s) 125, 290, 327, 611, 656, 736, 759, 964
BsuTUI ATCGAT 1 cut(s) 552
BtgZI GCGATG 1 cut(s) 821
BtrI CACGTC 2 cut(s) 410, 732
BtsCI GGATG 1 cut(s) 423
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 3 cut(s) 223, 500, 650
BtuMI TCGCGA 1 cut(s) 374
BveI ACCTGC 1 cut(s) 1072
Cac8I GCNNGC 4 cut(s) 306, 372, 666, 1092
CaiI CAGNNNCTG 2 cut(s) 38, 1087
CfoI GCGC 2 cut(s) 174, 310
Cfr13I GGNCC 4 cut(s) 325, 462, 498, 735
Cfr9I CCCGGG 1 cut(s) 588
ClaI ATCGAT 1 cut(s) 552
CseI GACGC 3 cut(s) 452, 548, 791
Csp6I GTAC 3 cut(s) 157, 712, 922
CspCI CAANNNNNGTGG 2 cut(s) 1058, 1093
CviAII CATG 5 cut(s) 505, 514, 642, 742, 915
CviQI GTAC 3 cut(s) 157, 712, 922
DdeI CTNAG 3 cut(s) 39, 90, 956
DpnI GATC 4 cut(s) 439, 529, 563, 605
DpnII GATC 4 cut(s) 437, 527, 561, 603
DraIII CACNNNGTG 1 cut(s) 697
EaeI YGGCCR 3 cut(s) 123, 288, 654
Eco31I GGTCTC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 462, 498
Eco47III AGCGCT 1 cut(s) 309
Eco72I CACGTG 1 cut(s) 694
Eco88I CYCGRG 1 cut(s) 588
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 3 cut(s) 103, 290, 759
FaeI CATG 5 cut(s) 508, 517, 645, 745, 918
FaqI GGGAC 2 cut(s) 684, 947
FatI CATG 5 cut(s) 504, 513, 641, 741, 914
FauI CCCGC 4 cut(s) 255, 535, 661, 923
FauNDI CATATG 1 cut(s) 1009
FblI GTMKAC 3 cut(s) 474, 765, 894
Fnu4HI GCNGC 7 cut(s) 6, 70, 73, 76, 79, 251, 757
FokI GGATG 1 cut(s) 430
Fsp4HI GCNGC 7 cut(s) 6, 70, 73, 76, 79, 251, 757
FspBI CTAG 1 cut(s) 1125
GlaI GCGC 2 cut(s) 173, 309
GluI GCNGC 7 cut(s) 6, 70, 73, 76, 79, 251, 757
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 8 cut(s) 125, 290, 327, 611, 656, 736, 759, 964
HapII CCGG 4 cut(s) 212, 353, 383, 589
HgaI GACGC 3 cut(s) 452, 548, 791
HhaI GCGC 2 cut(s) 174, 310
Hin1II CATG 5 cut(s) 508, 517, 645, 745, 918
Hin6I GCGC 2 cut(s) 172, 308
HinP1I GCGC 2 cut(s) 172, 308
HindIII AAGCTT 1 cut(s) 162
HpaII CCGG 4 cut(s) 212, 353, 383, 589
HphI GGTGA 2 cut(s) 709, 1073
Hpy166II GTNNAC 7 cut(s) 380, 465, 475, 729, 766, 822, 895
Hpy188III TCNNGA 2 cut(s) 113, 373
Hpy8I GTNNAC 7 cut(s) 380, 465, 475, 729, 766, 822, 895
Hpy99I CGWCG 1 cut(s) 289
HpyAV CCTTC 7 cut(s) 155, 283, 748, 809, 883, 1026, 1131
HpyCH4IV ACGT 6 cut(s) 51, 409, 693, 714, 731, 924
HpyCH4V TGCA 2 cut(s) 23, 228
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 75, 78, 250, 653, 674, 1087, 1100
HpyF3I CTNAG 3 cut(s) 39, 90, 956
HpySE526I ACGT 6 cut(s) 51, 409, 693, 714, 731, 924
Hsp92II CATG 5 cut(s) 508, 517, 645, 745, 918
HspAI GCGC 2 cut(s) 172, 308
Kzo9I GATC 4 cut(s) 437, 527, 561, 603
LmnI GCTCC 5 cut(s) 60, 249, 514, 990, 1099
Lsp1109I GCAGC 6 cut(s) 17, 81, 84, 87, 90, 237
LweI GCATC 5 cut(s) 32, 339, 408, 558, 651
MaeI CTAG 1 cut(s) 1125
MaeII ACGT 6 cut(s) 51, 409, 693, 714, 731, 924
MaeIII GTNAC 3 cut(s) 234, 715, 779
MalI GATC 4 cut(s) 439, 529, 563, 605
MboI GATC 4 cut(s) 437, 527, 561, 603
MboII GAAGA 2 cut(s) 128, 212
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 1 cut(s) 43
MluI ACGCGT 1 cut(s) 783
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 6 cut(s) 385, 593, 680, 764, 808, 944
MmeI TCCRAC 1 cut(s) 553
MnlI CCTC 9 cut(s) 28, 186, 327, 354, 409, 569, 715, 804, 979
Mox20I TGGCCA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 656
MslI CAYNNNNRTG 1 cut(s) 998
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 2 cut(s) 250, 668
MspI CCGG 4 cut(s) 212, 353, 383, 589
MspR9I CCNGG 8 cut(s) 105, 212, 292, 354, 384, 589, 590, 761
MvaI CCWGG 3 cut(s) 105, 292, 761
MvnI CGCG 5 cut(s) 374, 446, 542, 756, 785
MwoI GCNNNNNNNGC 8 cut(s) 69, 75, 78, 250, 653, 674, 1087, 1100
NciI CCSGG 5 cut(s) 212, 354, 384, 589, 590
NdeI CATATG 1 cut(s) 1009
NdeII GATC 4 cut(s) 437, 527, 561, 603
NlaIII CATG 5 cut(s) 508, 517, 645, 745, 918
NlaIV GGNNCC 2 cut(s) 510, 586
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 3 cut(s) 234, 715, 779
NruI TCGCGA 1 cut(s) 374
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 975
PfeI GAWTC 4 cut(s) 100, 295, 449, 1043
PflMI CCANNNNNTGG 3 cut(s) 166, 495, 697
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 7 cut(s) 7, 71, 74, 77, 80, 252, 758
Ple19I CGATCG 1 cut(s) 530
PleI GAGTC 6 cut(s) 384, 593, 680, 764, 807, 944
PmaCI CACGTG 1 cut(s) 694
PmlI CACGTG 1 cut(s) 694
PpsI GAGTC 6 cut(s) 384, 593, 680, 764, 807, 944
Ppu21I YACGTR 1 cut(s) 694
PshAI GACNNNNGTC 1 cut(s) 827
PsiI TTATAA 1 cut(s) 624
Psp6I CCWGG 3 cut(s) 103, 290, 759
PspCI CACGTG 1 cut(s) 694
PspGI CCWGG 3 cut(s) 103, 290, 759
PspN4I GGNNCC 2 cut(s) 510, 586
PspPI GGNCC 4 cut(s) 325, 462, 498, 735
PstNI CAGNNNCTG 2 cut(s) 38, 1087
PvuI CGATCG 1 cut(s) 530
RruI TCGCGA 1 cut(s) 374
RsaI GTAC 3 cut(s) 158, 713, 923
RsaNI GTAC 3 cut(s) 157, 712, 922
RseI CAYNNNNRTG 1 cut(s) 998
SatI GCNGC 7 cut(s) 6, 70, 73, 76, 79, 251, 757
Sau3AI GATC 4 cut(s) 437, 527, 561, 603
Sau96I GGNCC 4 cut(s) 325, 462, 498, 735
SchI GAGTC 6 cut(s) 385, 593, 680, 764, 808, 944
ScrFI CCNGG 8 cut(s) 105, 212, 292, 354, 384, 589, 590, 761
SfaNI GCATC 5 cut(s) 32, 339, 408, 558, 651
SinI GGWCC 2 cut(s) 462, 498
SmaI CCCGGG 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 998
Sse9I AATT 1 cut(s) 43
SspMI CTAG 1 cut(s) 1125
StyD4I CCNGG 8 cut(s) 103, 210, 290, 352, 382, 587, 588, 759
TaiI ACGT 6 cut(s) 54, 412, 696, 717, 734, 927
TaqI TCGA 4 cut(s) 284, 312, 552, 602
TaqII GACCGA 1 cut(s) 166
TasI AATT 1 cut(s) 43
TauI GCSGC 1 cut(s) 759
TfiI GAWTC 4 cut(s) 100, 295, 449, 1043
TscAI CASTG 3 cut(s) 230, 500, 657
TseFI GTSAC 3 cut(s) 234, 715, 779
TseI GCWGC 6 cut(s) 5, 69, 72, 75, 78, 250
Tsp45I GTSAC 3 cut(s) 234, 715, 779
TspDTI ATGAA 2 cut(s) 730, 902
TspGWI ACGGA 2 cut(s) 138, 144
TspMI CCCGGG 1 cut(s) 588
TspRI CASTG 3 cut(s) 230, 500, 657
Van91I CCANNNNNTGG 3 cut(s) 166, 495, 697
VpaK11BI GGWCC 2 cut(s) 462, 498
XapI RAATTY 1 cut(s) 43
XcmI CCANNNNNNNNNTGG 1 cut(s) 327
XmaI CCCGGG 1 cut(s) 588
XmiI GTMKAC 3 cut(s) 474, 765, 894
XspI CTAG 1 cut(s) 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.