pycom08g10690

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013470
Physical Location & Seq
Reverse (-)
8931299 .. 8932438
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g10690.1

Sequence Viewer

Length: 1140 bp
ATGTCGAAAATGGCGGCTCCGAAATCATCATCTGGATCAAACCCAGGTTCTGATCAGTGTTCTAACCAAGAAATCAGCATGAACAAATCTGAGCCGTTGATTCCCGGATTGCCGGACGAGGTCGCCGAGCTCTGCCTGCTCTATCTTCCCTATCCGTACCAAGCTTTGGTGCGTTCGGTTTCTGCTTCATGGAACAGAGCCATTACAGACCCTAGCTTTGTTCTTCACAAGAAGTCTTTGTCTTTGCCTCTGCCTTATCTTTTCGTTTTGGCCTTCAACAAATCAACGGCCATGATCCAGTGGCAAGCCCTGGACCCACGATCTGGCCGTTGGTTTGTTTTGCCTCCCATGCCGTGTCCCAAGGCCACTTGCCCGCCGGGGTTTGCATGCGCTTCCCTGCCACGTGAGGGGACGCTTGTTGTAGCGGGCGGCGTGCGGTCGGACACAGAATGTTCTATGGAAACAACCTTTGTGTATCGAACATCTACAAATCAATGGTCGACAGCCGCTCCAATGCGGACCCCACGGTCATTTTTCGACGCTGGAAATATCAACGGAAAGATCTTGGCCGTCGGAGGCGGCAAGGATCACTATGGCAGCTCGATCACGGCCGTGGACTGTTACGACCCTAAAAAAGACACGTGGGAGGCATATGCCCCTCTGCCAGGTGGACTGGCCAAGTATGATTCCAATGTGGTGGGCAACAAGATGTACGTGACCGAGGGGTGGACGTGGCCGTTTATGTACTCGCCACGTGGCGTGGTCTACGACCCTGACGATAACACGTGGCAAGAGATGAGGCCGGGAATGAGGGACGGATGGACGGGCGTGAGCGTGGTCGTGGGTGGCAGGCTGTTGGTGATATCGGAGTACGGGGACTGTCCGATGAAGGCGTACGACCCTGATAAGGACACGTGGCGGTACGTGGGTGGGGACAGGTTCCCATGTGAGGCACTGAGACGCCCCTTTGCTGCGAGCGGGGTAGAAGGGAACATATATGTGGTGGCATGTGGGTTGAACGTGGGCGTAGGAAGGCTGTCCGAGTGTGAAGCAGAGCTAAAAGTTGAGTGGCAGCTCCTGCCAGCTCCAAGTGCTTTTAGGGGTTTTTCTCCTTCTAGTTGTCAAGTGCTTCTTGCCTAA

Protein Analysis

380

Amino Acids

41.4

Weight (kDa)

5.86

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 102 - 217 7.7e-06 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 103 - 239 5.7e-07 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 110 - 216 5e-08 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 136 - 334 6.2e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 141 - 268 5e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 151 - 311 2.6e-17 FKB95, Kelch-repeats domain
Kelch_1 PF01344 175 - 222 4e-08 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24540
fragaria_vesca FvH4_2g36671
malus_domestica MD08G1127900.v1.1 MD15G1107400.v1.1
prunus_persica Prupe.1G459700_v2.0.a1 Prupe.5G016700_v2.0.a1
pyrus_communis pycom08g10690 pycom15g09820
rosa_chinensis RchiOBHm_Chr6g0305031
rosa_laevigata RLG00000010926
rosa_multiflora Rmu_sc0009145.1_g000002
rosa_roxburghii Rroxscaffold_7G00163240
rosa_rugosa Rorug06G0336900
rosa_samantha Rh6AG448500 Rh6BG452400 Rh6CG462200 Rh6DG449100
rosa_wichuraiana Rw6G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 526
AccB7I CCANNNNNTGG 2 cut(s) 166, 323
AccBSI CCGCTC 2 cut(s) 509, 978
AccI GTMKAC 2 cut(s) 500, 765
AclWI GGATC 3 cut(s) 43, 289, 594
AcoI YGGCCR 6 cut(s) 288, 325, 567, 609, 675, 734
AcvI CACGTG 5 cut(s) 404, 642, 755, 786, 915
AcyI GRCGYC 1 cut(s) 961
AfaI GTAC 6 cut(s) 158, 713, 746, 872, 896, 923
AfiI CCNNNNNNNGG 7 cut(s) 166, 323, 407, 665, 726, 907, 949
AflIII ACRYGT 3 cut(s) 639, 783, 912
AgsI TTSAA 2 cut(s) 277, 1018
AjiI CACGTC 1 cut(s) 732
AjnI CCWGG 3 cut(s) 43, 309, 664
AleI CACNNNNGTG 1 cut(s) 611
AluBI AGCT 7 cut(s) 130, 164, 216, 600, 1057, 1075, 1085
AluI AGCT 7 cut(s) 130, 164, 216, 600, 1057, 1075, 1085
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 952
AlwI GGATC 3 cut(s) 43, 289, 594
AlwNI CAGNNNCTG 2 cut(s) 50, 1078
AoxI GGCC 9 cut(s) 270, 288, 325, 363, 567, 609, 675, 734, 800
ApeKI GCWGC 3 cut(s) 597, 971, 1072
ArsI GACNNNNNNTTYG 4 cut(s) 200, 232, 482, 514
AspLEI GCGC 1 cut(s) 392
AspS9I GGNCC 2 cut(s) 313, 519
AsuC2I CCSGG 3 cut(s) 105, 378, 804
AsuHPI GGTGA 1 cut(s) 871
AvaII GGWCC 2 cut(s) 313, 519
BaeI ACNNNNGTAYC 2 cut(s) 913, 946
BalI TGGCCA 1 cut(s) 677
BanII GRGCYC 1 cut(s) 132
BbrPI CACGTG 5 cut(s) 404, 642, 755, 786, 915
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 609, 958, 1084
BccI CCATC 1 cut(s) 813
BceAI ACGGC 8 cut(s) 79, 303, 312, 337, 554, 596, 624, 721
BciT130I CCWGG 3 cut(s) 45, 311, 666
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 3 cut(s) 105, 378, 804
BcoDI GTCTC 1 cut(s) 952
BfaI CTAG 2 cut(s) 213, 1116
BglII AGATCT 1 cut(s) 561
BisI GCNGC 7 cut(s) 15, 430, 507, 580, 598, 972, 1073
BlsI GCNGC 7 cut(s) 16, 431, 508, 581, 599, 973, 1074
Bme1390I CCNGG 6 cut(s) 45, 105, 311, 378, 666, 804
Bme18I GGWCC 2 cut(s) 313, 519
BmgBI CACGTC 1 cut(s) 732
BmgT120I GGNCC 2 cut(s) 313, 519
BmiI GGNNCC 4 cut(s) 18, 315, 521, 941
BmrFI CCNGG 6 cut(s) 45, 105, 311, 378, 666, 804
BpuMI CCSGG 3 cut(s) 105, 378, 804
BsaAI YACGTR 7 cut(s) 404, 642, 715, 755, 786, 915, 925
BsaHI GRCGYC 1 cut(s) 961
BsaJI CCNNGG 7 cut(s) 43, 309, 360, 377, 524, 612, 720
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bsc4I CCNNNNNNNGG 7 cut(s) 166, 323, 407, 665, 726, 907, 949
Bse1I ACTGG 2 cut(s) 298, 678
BseBI CCWGG 3 cut(s) 45, 311, 666
BseDI CCNNGG 7 cut(s) 43, 309, 360, 377, 524, 612, 720
BseGI GGATG 1 cut(s) 824
BseLI CCNNNNNNNGG 7 cut(s) 166, 323, 407, 665, 726, 907, 949
BseMII CTCAG 2 cut(s) 81, 947
BseNI ACTGG 2 cut(s) 298, 678
BseX3I CGGCCG 1 cut(s) 609
BseXI GCAGC 3 cut(s) 609, 958, 1084
Bsh1285I CGRYCG 2 cut(s) 440, 612
BshFI GGCC 9 cut(s) 272, 290, 327, 365, 569, 611, 677, 736, 802
BsiEI CGRYCG 2 cut(s) 440, 612
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 4 cut(s) 105, 113, 377, 803
BsiWI CGTACG 1 cut(s) 894
BslFI GGGAC 5 cut(s) 342, 424, 827, 890, 947
BslI CCNNNNNNNGG 7 cut(s) 166, 323, 407, 665, 726, 907, 949
BsmAI GTCTC 1 cut(s) 952
BsmBI CGTCTC 1 cut(s) 952
BsmFI GGGAC 5 cut(s) 342, 424, 827, 890, 947
BsnI GGCC 9 cut(s) 272, 290, 327, 365, 569, 611, 677, 736, 802
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 7 cut(s) 35, 52, 294, 320, 561, 586, 603
BspANI GGCC 9 cut(s) 272, 290, 327, 365, 569, 611, 677, 736, 802
BspCNI CTCAG 2 cut(s) 82, 948
BspLI GGNNCC 4 cut(s) 18, 315, 521, 941
BspPI GGATC 3 cut(s) 43, 289, 594
BsrBI CCGCTC 2 cut(s) 509, 978
BsrI ACTGG 2 cut(s) 298, 678
BssECI CCNNGG 7 cut(s) 43, 309, 360, 377, 524, 612, 720
BssMI GATC 7 cut(s) 35, 52, 294, 320, 561, 586, 603
BssNI GRCGYC 1 cut(s) 961
BssT1I CCWWGG 1 cut(s) 360
Bst2UI CCWGG 3 cut(s) 45, 311, 666
Bst4CI ACNGT 3 cut(s) 528, 620, 881
BstACI GRCGYC 1 cut(s) 961
BstAPI GCANNNNNTGC 1 cut(s) 1078
BstBAI YACGTR 7 cut(s) 404, 642, 715, 755, 786, 915, 925
BstC8I GCNNGC 9 cut(s) 137, 306, 374, 388, 427, 434, 851, 976, 1083
BstDEI CTNAG 2 cut(s) 90, 956
BstDSI CCRYGG 2 cut(s) 524, 612
BstENI CCTNNNNNAGG 1 cut(s) 663
BstF5I GGATG 1 cut(s) 824
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 7 cut(s) 38, 55, 297, 323, 564, 589, 606
BstMAI GTCTC 1 cut(s) 952
BstMBI GATC 7 cut(s) 35, 52, 294, 320, 561, 586, 603
BstMCI CGRYCG 2 cut(s) 440, 612
BstMWI GCNNNNNNNGC 4 cut(s) 136, 349, 1078, 1091
BstNI CCWGG 3 cut(s) 45, 311, 666
BstNSI RCATGY 2 cut(s) 390, 1011
BstSCI CCNGG 6 cut(s) 43, 103, 309, 376, 664, 802
BstV1I GCAGC 3 cut(s) 609, 958, 1084
BstX2I RGATCY 1 cut(s) 561
BstXI CCANNNNNNTGG 1 cut(s) 697
BstYI RGATCY 1 cut(s) 561
BstZI CGGCCG 1 cut(s) 609
BsuRI GGCC 9 cut(s) 272, 290, 327, 365, 569, 611, 677, 736, 802
BtgI CCRYGG 2 cut(s) 524, 612
BtrI CACGTC 1 cut(s) 732
BtsCI GGATG 1 cut(s) 824
BtsIMutI CAGTG 3 cut(s) 62, 305, 953
Cac8I GCNNGC 9 cut(s) 137, 306, 374, 388, 427, 434, 851, 976, 1083
CaiI CAGNNNCTG 2 cut(s) 50, 1078
CfoI GCGC 1 cut(s) 392
Cfr13I GGNCC 2 cut(s) 313, 519
CseI GACGC 3 cut(s) 421, 548, 969
Csp6I GTAC 6 cut(s) 157, 712, 745, 871, 895, 922
CviAII CATG 7 cut(s) 79, 189, 292, 349, 387, 945, 1008
CviQI GTAC 6 cut(s) 157, 712, 745, 871, 895, 922
DdeI CTNAG 2 cut(s) 90, 956
DpnI GATC 7 cut(s) 37, 54, 296, 322, 563, 588, 605
DpnII GATC 7 cut(s) 35, 52, 294, 320, 561, 586, 603
DrdI GACNNNNNNGTC 1 cut(s) 526
DseDI GACNNNNNNGTC 1 cut(s) 526
EaeI YGGCCR 6 cut(s) 288, 325, 567, 609, 675, 734
EagI CGGCCG 1 cut(s) 609
Ecl136II GAGCTC 1 cut(s) 130
EclXI CGGCCG 1 cut(s) 609
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 864
Eco47I GGWCC 2 cut(s) 313, 519
Eco52I CGGCCG 1 cut(s) 609
Eco53kI GAGCTC 1 cut(s) 130
Eco72I CACGTG 5 cut(s) 404, 642, 755, 786, 915
EcoICRI GAGCTC 1 cut(s) 130
EcoNI CCTNNNNNAGG 1 cut(s) 663
EcoRII CCWGG 3 cut(s) 43, 309, 664
EcoRV GATATC 1 cut(s) 864
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 360
Esp3I CGTCTC 1 cut(s) 952
FaeI CATG 7 cut(s) 82, 192, 295, 352, 390, 948, 1011
FalI AAGNNNNNCTT 1 cut(s) 1116
FaqI GGGAC 5 cut(s) 342, 424, 827, 890, 947
FatI CATG 7 cut(s) 78, 188, 291, 348, 386, 944, 1007
FauI CCCGC 3 cut(s) 381, 418, 971
FauNDI CATATG 1 cut(s) 652
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 2 cut(s) 500, 765
Fnu4HI GCNGC 7 cut(s) 15, 430, 507, 580, 598, 972, 1073
FokI GGATG 1 cut(s) 831
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 7 cut(s) 15, 430, 507, 580, 598, 972, 1073
FspBI CTAG 2 cut(s) 213, 1116
GlaI GCGC 1 cut(s) 391
GluI GCNGC 7 cut(s) 15, 430, 507, 580, 598, 972, 1073
HaeIII GGCC 9 cut(s) 272, 290, 327, 365, 569, 611, 677, 736, 802
HapII CCGG 4 cut(s) 105, 113, 377, 803
HgaI GACGC 3 cut(s) 421, 548, 969
HhaI GCGC 1 cut(s) 392
Hin1I GRCGYC 1 cut(s) 961
Hin1II CATG 7 cut(s) 82, 192, 295, 352, 390, 948, 1011
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HindIII AAGCTT 1 cut(s) 162
HinfI GANTC 2 cut(s) 100, 686
HpaII CCGG 4 cut(s) 105, 113, 377, 803
HphI GGTGA 1 cut(s) 871
Hpy166II GTNNAC 5 cut(s) 501, 616, 671, 729, 766
Hpy188I TCNGA 8 cut(s) 21, 52, 91, 442, 575, 868, 885, 1042
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 5 cut(s) 501, 616, 671, 729, 766
Hpy99I CGWCG 2 cut(s) 542, 575
HpyAV CCTTC 5 cut(s) 283, 883, 980, 1026, 1122
HpyCH4III ACNGT 3 cut(s) 528, 620, 881
HpyCH4IV ACGT 9 cut(s) 403, 641, 714, 731, 754, 785, 914, 924, 1020
HpyCH4V TGCA 1 cut(s) 386
HpyF10VI GCNNNNNNNGC 4 cut(s) 136, 349, 1078, 1091
HpyF3I CTNAG 2 cut(s) 90, 956
HpySE526I ACGT 9 cut(s) 403, 641, 714, 731, 754, 785, 914, 924, 1020
Hsp92I GRCGYC 1 cut(s) 961
Hsp92II CATG 7 cut(s) 82, 192, 295, 352, 390, 948, 1011
HspAI GCGC 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 7 cut(s) 35, 52, 294, 320, 561, 586, 603
LmnI GCTCC 4 cut(s) 22, 514, 1080, 1090
Lsp1109I GCAGC 3 cut(s) 609, 958, 1084
MaeI CTAG 2 cut(s) 213, 1116
MaeII ACGT 9 cut(s) 403, 641, 714, 731, 754, 785, 914, 924, 1020
MaeIII GTNAC 2 cut(s) 620, 715
MalI GATC 7 cut(s) 37, 54, 296, 322, 563, 588, 605
MbiI CCGCTC 2 cut(s) 509, 978
MboI GATC 7 cut(s) 35, 52, 294, 320, 561, 586, 603
MboII GAAGA 2 cut(s) 137, 215
MflI RGATCY 1 cut(s) 561
MhlI GDGCHC 1 cut(s) 132
MlsI TGGCCA 1 cut(s) 677
MluNI TGGCCA 1 cut(s) 677
MmeI TCCRAC 2 cut(s) 420, 553
Mox20I TGGCCA 1 cut(s) 677
MscI TGGCCA 1 cut(s) 677
MslI CAYNNNNRTG 2 cut(s) 611, 998
Msp20I TGGCCA 1 cut(s) 677
MspI CCGG 4 cut(s) 105, 113, 377, 803
MspR9I CCNGG 6 cut(s) 45, 105, 311, 378, 666, 804
MvaI CCWGG 3 cut(s) 45, 311, 666
MwoI GCNNNNNNNGC 4 cut(s) 136, 349, 1078, 1091
NciI CCSGG 3 cut(s) 105, 378, 804
NdeI CATATG 1 cut(s) 652
NdeII GATC 7 cut(s) 35, 52, 294, 320, 561, 586, 603
NlaIII CATG 7 cut(s) 82, 192, 295, 352, 390, 948, 1011
NlaIV GGNNCC 4 cut(s) 18, 315, 521, 941
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 715
NspI RCATGY 2 cut(s) 390, 1011
OliI CACNNNNGTG 1 cut(s) 611
PaeI GCATGC 1 cut(s) 390
PcsI WCGNNNNNNNCGW 3 cut(s) 123, 325, 774
PfeI GAWTC 2 cut(s) 100, 686
Pfl23II CGTACG 1 cut(s) 894
PflFI GACNNNGTC 1 cut(s) 119
PflMI CCANNNNNTGG 2 cut(s) 166, 323
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 7 cut(s) 16, 431, 508, 581, 599, 973, 1074
PmaCI CACGTG 5 cut(s) 404, 642, 755, 786, 915
PmlI CACGTG 5 cut(s) 404, 642, 755, 786, 915
Ppu21I YACGTR 7 cut(s) 404, 642, 715, 755, 786, 915, 925
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 3 cut(s) 43, 309, 664
PspCI CACGTG 5 cut(s) 404, 642, 755, 786, 915
PspGI CCWGG 3 cut(s) 43, 309, 664
PspLI CGTACG 1 cut(s) 894
PspN4I GGNNCC 4 cut(s) 18, 315, 521, 941
PspPI GGNCC 2 cut(s) 313, 519
PstNI CAGNNNCTG 2 cut(s) 50, 1078
PsuI RGATCY 1 cut(s) 561
PsyI GACNNNGTC 1 cut(s) 119
RsaI GTAC 6 cut(s) 158, 713, 746, 872, 896, 923
RsaNI GTAC 6 cut(s) 157, 712, 745, 871, 895, 922
RseI CAYNNNNRTG 2 cut(s) 611, 998
SacI GAGCTC 1 cut(s) 132
SalI GTCGAC 1 cut(s) 499
SatI GCNGC 7 cut(s) 15, 430, 507, 580, 598, 972, 1073
Sau3AI GATC 7 cut(s) 35, 52, 294, 320, 561, 586, 603
Sau96I GGNCC 2 cut(s) 313, 519
ScrFI CCNGG 6 cut(s) 45, 105, 311, 378, 666, 804
SduI GDGCHC 1 cut(s) 132
SinI GGWCC 2 cut(s) 313, 519
SmiMI CAYNNNNRTG 2 cut(s) 611, 998
SphI GCATGC 1 cut(s) 390
SspMI CTAG 2 cut(s) 213, 1116
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 6 cut(s) 43, 103, 309, 376, 664, 802
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 3 cut(s) 528, 620, 881
TaiI ACGT 9 cut(s) 406, 644, 717, 734, 757, 788, 917, 927, 1023
TaqI TCGA 5 cut(s) 5, 478, 500, 537, 602
TaqII GACCGA 1 cut(s) 734
TatI WGTACW 1 cut(s) 744
TauI GCSGC 4 cut(s) 17, 432, 509, 582
TfiI GAWTC 2 cut(s) 100, 686
TscAI CASTG 3 cut(s) 62, 305, 960
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 3 cut(s) 597, 971, 1072
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 3 cut(s) 95, 177, 902
TspGWI ACGGA 3 cut(s) 144, 570, 831
TspRI CASTG 3 cut(s) 62, 305, 960
Tth111I GACNNNGTC 1 cut(s) 119
Van91I CCANNNNNTGG 2 cut(s) 166, 323
VpaK11BI GGWCC 2 cut(s) 313, 519
XagI CCTNNNNNAGG 1 cut(s) 663
XceI RCATGY 2 cut(s) 390, 1011
XmiI GTMKAC 2 cut(s) 500, 765
XspI CTAG 2 cut(s) 213, 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.