FvH4_2g36671

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013470
Physical Location & Seq
Forward (+)
26806204 .. 26808510
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36671.t1

Sequence Viewer

Length: 1152 bp
ATGGGGTTTTCAACAATGGCAGCTTTGACTTCATCTGCATCAAACCCAGAAACTGAGAATTCTGACATAGAAAGGAGGAGAAGAAGCAGCAACAAATCTGAGCCGTTGATTCCTGGACTTCCCGACGAGGTGACCGAGCTGTGTCTGCTTTACCTTCCCTATCCATACCAAACTTTGGTGCGTTCGGTCTCTGCTTCGTGGAACAGAGCCATTTCAGACCCGGCTTTCCTTCACTGCAAGAAGTCTCTTTCGCTCCCGCTGCCGTATCTTTTCGTTTTCGCCTTCAACAAATCGACGGCCAGGATTCAGTGGCAAGCGCTAGACCCTCGATCGGGCCGTTGGTTTGTCCTACCTCCGATGCCGGGGCATTATTCCAAGGCTGGGAGTCCACCGGGGTTAGCATGCGCCTCCTTGCCACGTCAAGGGATGCTTGTCGTGTTGGGTGGATCCAACGCGGAATGTTCTATGCGGTCCACCCTCGTGTACCGTACATCGACCAATCAATGGTCCAACGCGGCTCTTATGCCGACTGCTCGATCTTATTTCGATGCAGGGTGCGTCGATAATAAAATCGTAGCAGTCGGAGGTACTGGGACATGCGAGAACGACTCAATTAGGACTGTCGAGTGTTACGACCCTGAAATGGATAAGTGGACGACAATTGCGACAATGCCTGTAGGACTTACCAAATACGACTCCCACGTGGTGGGGGATAAGATGTATGTCACTGAGGGGTGGACGTGGCCCTTTATGTTCTCGCCACGTAGCCTGGTCTACGACTCGAAGACGGACGTGTGGCGAGAAATGAGTCTCGGGATGAGGGAAGGCTGGACGGGCATCAGTGTATTAGTGGACAACAAGTTATTGGTGATATCGGAGTATGGTGATTGTCCGATTAAAGTGTACGATCATGATGAGGACACGTGGAGGTACGTCGGAGGGGACAAGTTCCCGTGCGACGCACTTAGACGGCCTTTCGCTGTAAGCGCGGTGGAGGGTAGCATATATGTGGTGGCATGTGGGCTGAATGTGGGAATAGGAAGGCTGTCTGAAGGAGAAAAGGGCGAGGTACAGGTCGAGTGGCAGGTTATGCCAGCTCCAAGTGCTTTTAGGGGTTTTTCTCCTTCTAGTTGTCAAGTGCTTTATGCCTGA

Protein Analysis

384

Amino Acids

42.36

Weight (kDa)

5.71

Isoelectric Point (pI)

56.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_Calicin PF13964 113 - 245 5.6e-12 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 133 - 271 1.8e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 138 - 295 3.8e-07 FBX42, beta-propeller domain
Kelch_FKB95 PF25210 146 - 314 1.1e-17 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 146 - 270 2.2e-06 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 178 - 225 1.3e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24540
fragaria_vesca FvH4_2g36671
malus_domestica MD08G1127900.v1.1 MD15G1107400.v1.1
prunus_persica Prupe.1G459700_v2.0.a1 Prupe.5G016700_v2.0.a1
pyrus_communis pycom08g10690 pycom15g09820
rosa_chinensis RchiOBHm_Chr6g0305031
rosa_laevigata RLG00000010926
rosa_multiflora Rmu_sc0009145.1_g000002
rosa_roxburghii Rroxscaffold_7G00163240
rosa_rugosa Rorug06G0336900
rosa_samantha Rh6AG448500 Rh6BG452400 Rh6CG462200 Rh6DG449100
rosa_wichuraiana Rw6G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1075
AccB7I CCANNNNNTGG 3 cut(s) 175, 504, 706
AccI GTMKAC 1 cut(s) 774
AccII CGCG 3 cut(s) 455, 515, 989
AciI CCGC 5 cut(s) 257, 455, 469, 515, 989
AclWI GGATC 2 cut(s) 441, 454
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 1071
AcvI CACGTG 2 cut(s) 703, 924
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 6 cut(s) 485, 490, 589, 905, 932, 1071
AfeI AGCGCT 1 cut(s) 318
AfiI CCNNNNNNNGG 9 cut(s) 175, 331, 332, 362, 381, 422, 504, 643, 706
AflIII ACRYGT 2 cut(s) 792, 921
AgsI TTSAA 2 cut(s) 12, 286
AjiI CACGTC 3 cut(s) 419, 741, 793
AjnI CCWGG 3 cut(s) 112, 299, 768
AleI CACNNNNGTG 1 cut(s) 479
AluBI AGCT 3 cut(s) 23, 139, 1097
AluI AGCT 3 cut(s) 23, 139, 1097
Alw26I GTCTC 3 cut(s) 193, 249, 815
AlwI GGATC 2 cut(s) 441, 454
AlwNI CAGNNNCTG 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 812
Aor51HI AGCGCT 1 cut(s) 318
AoxI GGCC 4 cut(s) 297, 334, 743, 971
ApeKI GCWGC 3 cut(s) 20, 87, 259
ApoI RAATTY 1 cut(s) 58
AspLEI GCGC 3 cut(s) 319, 407, 989
AspS9I GGNCC 4 cut(s) 334, 471, 507, 744
AsuC2I CCSGG 3 cut(s) 221, 363, 393
AsuHPI GGTGA 3 cut(s) 142, 880, 896
AvaI CYCGRG 1 cut(s) 812
AvaII GGWCC 2 cut(s) 471, 507
BamHI GGATCC 1 cut(s) 446
BauI CACGAG 1 cut(s) 479
BbrPI CACGTG 2 cut(s) 703, 924
BbsI GAAGAC 1 cut(s) 791
BbvI GCAGC 3 cut(s) 32, 99, 246
BceAI ACGGC 5 cut(s) 88, 247, 312, 321, 986
BciT130I CCWGG 3 cut(s) 114, 301, 770
BcnI CCSGG 3 cut(s) 221, 363, 393
BcoDI GTCTC 3 cut(s) 193, 249, 815
BfaI CTAG 2 cut(s) 320, 1128
BfmI CTRYAG 1 cut(s) 675
BfoI RGCGCY 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 1075
BisI GCNGC 4 cut(s) 21, 88, 260, 516
BlsI GCNGC 4 cut(s) 22, 89, 261, 517
Bme1390I CCNGG 6 cut(s) 114, 221, 301, 363, 393, 770
Bme18I GGWCC 2 cut(s) 471, 507
BmeT110I CYCGRG 1 cut(s) 812
BmgBI CACGTC 3 cut(s) 419, 741, 793
BmgT120I GGNCC 4 cut(s) 334, 471, 507, 744
BmiI GGNNCC 1 cut(s) 448
BmrFI CCNGG 6 cut(s) 114, 221, 301, 363, 393, 770
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 5 cut(s) 47, 348, 417, 538, 846
BmuI ACTGGG 1 cut(s) 600
BpiI GAAGAC 1 cut(s) 791
BplI GAGNNNNNCTC 2 cut(s) 593, 625
BpuMI CCSGG 3 cut(s) 221, 363, 393
BsaAI YACGTR 3 cut(s) 703, 764, 924
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 3 cut(s) 362, 375, 392
Bsc4I CCNNNNNNNGG 9 cut(s) 175, 331, 332, 362, 381, 422, 504, 643, 706
Bse1I ACTGG 1 cut(s) 595
BseBI CCWGG 3 cut(s) 114, 301, 770
BseDI CCNNGG 3 cut(s) 362, 375, 392
BseGI GGATG 2 cut(s) 432, 822
BseLI CCNNNNNNNGG 9 cut(s) 175, 331, 332, 362, 381, 422, 504, 643, 706
BseMII CTCAG 3 cut(s) 45, 90, 720
BseNI ACTGG 1 cut(s) 595
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 3 cut(s) 32, 99, 246
BseYI CCCAGC 1 cut(s) 380
Bsh1236I CGCG 3 cut(s) 455, 515, 989
Bsh1285I CGRYCG 1 cut(s) 332
BshFI GGCC 4 cut(s) 299, 336, 745, 973
BsiEI CGRYCG 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 812
BsiSI CCGG 3 cut(s) 221, 362, 392
BslFI GGGAC 2 cut(s) 607, 956
BslI CCNNNNNNNGG 9 cut(s) 175, 331, 332, 362, 381, 422, 504, 643, 706
BsmAI GTCTC 3 cut(s) 193, 249, 815
BsmFI GGGAC 2 cut(s) 607, 956
BsnI GGCC 4 cut(s) 299, 336, 745, 973
Bso31I GGTCTC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 812
Bsp143I GATC 4 cut(s) 329, 446, 536, 907
BspACI CCGC 5 cut(s) 257, 455, 469, 515, 989
BspANI GGCC 4 cut(s) 299, 336, 745, 973
BspCNI CTCAG 3 cut(s) 46, 91, 721
BspFNI CGCG 3 cut(s) 455, 515, 989
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 1 cut(s) 448
BspMI ACCTGC 1 cut(s) 1075
BspPI GGATC 2 cut(s) 441, 454
BspTNI GGTCTC 1 cut(s) 193
BsrI ACTGG 1 cut(s) 595
BssECI CCNNGG 3 cut(s) 362, 375, 392
BssMI GATC 4 cut(s) 329, 446, 536, 907
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 3 cut(s) 114, 301, 770
Bst4CI ACNGT 2 cut(s) 488, 622
BstAPI GCANNNNNTGC 1 cut(s) 1090
BstBAI YACGTR 3 cut(s) 703, 764, 924
BstC8I GCNNGC 3 cut(s) 315, 403, 1095
BstDEI CTNAG 4 cut(s) 54, 99, 729, 965
BstEII GGTNACC 1 cut(s) 130
BstF5I GGATG 2 cut(s) 432, 822
BstFNI CGCG 3 cut(s) 455, 515, 989
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 3 cut(s) 319, 407, 989
BstKTI GATC 4 cut(s) 332, 449, 539, 910
BstMAI GTCTC 3 cut(s) 193, 249, 815
BstMBI GATC 4 cut(s) 329, 446, 536, 907
BstMCI CGRYCG 1 cut(s) 332
BstMWI GCNNNNNNNGC 6 cut(s) 145, 259, 834, 986, 1090, 1103
BstNI CCWGG 3 cut(s) 114, 301, 770
BstNSI RCATGY 3 cut(s) 405, 600, 1020
BstPI GGTNACC 1 cut(s) 130
BstSCI CCNGG 6 cut(s) 112, 219, 299, 361, 391, 768
BstSFI CTRYAG 1 cut(s) 675
BstUI CGCG 3 cut(s) 455, 515, 989
BstV1I GCAGC 3 cut(s) 32, 99, 246
BstV2I GAAGAC 1 cut(s) 791
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BsuRI GGCC 4 cut(s) 299, 336, 745, 973
BtrI CACGTC 3 cut(s) 419, 741, 793
BtsCI GGATG 2 cut(s) 432, 822
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 4 cut(s) 232, 314, 726, 847
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 3 cut(s) 315, 403, 1095
CaiI CAGNNNCTG 1 cut(s) 53
CciI TCATGA 1 cut(s) 910
CfoI GCGC 3 cut(s) 319, 407, 989
Cfr13I GGNCC 4 cut(s) 334, 471, 507, 744
CseI GACGC 2 cut(s) 547, 968
Csp6I GTAC 6 cut(s) 484, 489, 588, 904, 931, 1070
CviAII CATG 4 cut(s) 402, 597, 911, 1017
CviQI GTAC 6 cut(s) 484, 489, 588, 904, 931, 1070
DdeI CTNAG 4 cut(s) 54, 99, 729, 965
DpnI GATC 4 cut(s) 331, 448, 538, 909
DpnII GATC 4 cut(s) 329, 446, 536, 907
DraIII CACNNNGTG 1 cut(s) 706
EaeI YGGCCR 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 193
Eco32I GATATC 1 cut(s) 873
Eco47I GGWCC 2 cut(s) 471, 507
Eco47III AGCGCT 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 1071
Eco72I CACGTG 2 cut(s) 703, 924
Eco88I CYCGRG 1 cut(s) 812
Eco91I GGTNACC 1 cut(s) 130
EcoO65I GGTNACC 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 58
EcoRII CCWGG 3 cut(s) 112, 299, 768
EcoRV GATATC 1 cut(s) 873
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 4 cut(s) 405, 600, 914, 1020
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FaqI GGGAC 2 cut(s) 607, 956
FatI CATG 4 cut(s) 401, 596, 910, 1016
FauI CCCGC 1 cut(s) 264
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 4 cut(s) 21, 88, 260, 516
FokI GGATG 2 cut(s) 439, 829
Fsp4HI GCNGC 4 cut(s) 21, 88, 260, 516
FspBI CTAG 2 cut(s) 320, 1128
GlaI GCGC 3 cut(s) 318, 406, 988
GluI GCNGC 4 cut(s) 21, 88, 260, 516
GsaI CCCAGC 1 cut(s) 384
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 4 cut(s) 299, 336, 745, 973
HapII CCGG 3 cut(s) 221, 362, 392
HgaI GACGC 2 cut(s) 547, 968
HhaI GCGC 3 cut(s) 319, 407, 989
Hin1II CATG 4 cut(s) 405, 600, 914, 1020
Hin6I GCGC 3 cut(s) 317, 405, 987
HinP1I GCGC 3 cut(s) 317, 405, 987
HinfI GANTC 7 cut(s) 109, 304, 385, 608, 695, 779, 808
HpaII CCGG 3 cut(s) 221, 362, 392
HphI GGTGA 3 cut(s) 142, 880, 896
Hpy166II GTNNAC 8 cut(s) 389, 474, 484, 654, 738, 775, 853, 904
Hpy188I TCNGA 9 cut(s) 64, 100, 217, 357, 584, 877, 894, 938, 1051
Hpy188III TCNNGA 3 cut(s) 122, 814, 911
Hpy8I GTNNAC 8 cut(s) 389, 474, 484, 654, 738, 775, 853, 904
Hpy99I CGWCG 5 cut(s) 128, 298, 563, 938, 962
HpyAV CCTTC 7 cut(s) 164, 239, 292, 818, 1035, 1046, 1134
HpyCH4III ACNGT 2 cut(s) 488, 622
HpyCH4IV ACGT 7 cut(s) 418, 702, 740, 763, 792, 923, 933
HpyCH4V TGCA 3 cut(s) 38, 237, 551
HpyF10VI GCNNNNNNNGC 6 cut(s) 145, 259, 834, 986, 1090, 1103
HpyF3I CTNAG 4 cut(s) 54, 99, 729, 965
HpySE526I ACGT 7 cut(s) 418, 702, 740, 763, 792, 923, 933
Hsp92II CATG 4 cut(s) 405, 600, 914, 1020
HspAI GCGC 3 cut(s) 317, 405, 987
Kzo9I GATC 4 cut(s) 329, 446, 536, 907
LmnI GCTCC 2 cut(s) 258, 1102
Lsp1109I GCAGC 3 cut(s) 32, 99, 246
LweI GCATC 5 cut(s) 47, 348, 417, 538, 846
MaeI CTAG 2 cut(s) 320, 1128
MaeII ACGT 7 cut(s) 418, 702, 740, 763, 792, 923, 933
MaeIII GTNAC 3 cut(s) 130, 629, 724
MalI GATC 4 cut(s) 331, 448, 538, 909
MboI GATC 4 cut(s) 329, 446, 536, 907
MboII GAAGA 2 cut(s) 93, 796
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 1 cut(s) 446
MluCI AATT 3 cut(s) 58, 612, 660
MlyI GAGTC 5 cut(s) 394, 602, 689, 773, 817
MmeI TCCRAC 4 cut(s) 474, 534, 562, 916
MseI TTAA 1 cut(s) 897
MslI CAYNNNNRTG 2 cut(s) 479, 1007
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 3 cut(s) 221, 362, 392
MspR9I CCNGG 6 cut(s) 114, 221, 301, 363, 393, 770
MunI CAATTG 1 cut(s) 660
MvaI CCWGG 3 cut(s) 114, 301, 770
MvnI CGCG 3 cut(s) 455, 515, 989
MwoI GCNNNNNNNGC 6 cut(s) 145, 259, 834, 986, 1090, 1103
NciI CCSGG 3 cut(s) 221, 363, 393
NdeII GATC 4 cut(s) 329, 446, 536, 907
NlaIII CATG 4 cut(s) 405, 600, 914, 1020
NlaIV GGNNCC 1 cut(s) 448
NmuCI GTSAC 2 cut(s) 130, 724
NspI RCATGY 3 cut(s) 405, 600, 1020
OliI CACNNNNGTG 1 cut(s) 479
PaeI GCATGC 1 cut(s) 405
PagI TCATGA 1 cut(s) 910
PcsI WCGNNNNNNNCGW 5 cut(s) 132, 334, 630, 662, 699
PfeI GAWTC 2 cut(s) 109, 304
PflMI CCANNNNNTGG 3 cut(s) 175, 504, 706
PfoI TCCNGGA 1 cut(s) 112
PkrI GCNGC 4 cut(s) 22, 89, 261, 517
Ple19I CGATCG 1 cut(s) 332
PleI GAGTC 5 cut(s) 393, 602, 689, 773, 816
PmaCI CACGTG 2 cut(s) 703, 924
PmlI CACGTG 2 cut(s) 703, 924
PpsI GAGTC 5 cut(s) 393, 602, 689, 773, 816
Ppu21I YACGTR 3 cut(s) 703, 764, 924
Psp6I CCWGG 3 cut(s) 112, 299, 768
PspCI CACGTG 2 cut(s) 703, 924
PspEI GGTNACC 1 cut(s) 130
PspFI CCCAGC 1 cut(s) 380
PspGI CCWGG 3 cut(s) 112, 299, 768
PspN4I GGNNCC 1 cut(s) 448
PspPI GGNCC 4 cut(s) 334, 471, 507, 744
PstNI CAGNNNCTG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 446
PvuI CGATCG 1 cut(s) 332
RsaI GTAC 6 cut(s) 485, 490, 589, 905, 932, 1071
RsaNI GTAC 6 cut(s) 484, 489, 588, 904, 931, 1070
RseI CAYNNNNRTG 2 cut(s) 479, 1007
SaqAI TTAA 1 cut(s) 897
SatI GCNGC 4 cut(s) 21, 88, 260, 516
Sau3AI GATC 4 cut(s) 329, 446, 536, 907
Sau96I GGNCC 4 cut(s) 334, 471, 507, 744
SchI GAGTC 5 cut(s) 394, 602, 689, 773, 817
ScrFI CCNGG 6 cut(s) 114, 221, 301, 363, 393, 770
SfaNI GCATC 5 cut(s) 47, 348, 417, 538, 846
SfcI CTRYAG 1 cut(s) 675
SinI GGWCC 2 cut(s) 471, 507
SmiMI CAYNNNNRTG 2 cut(s) 479, 1007
SphI GCATGC 1 cut(s) 405
Sse9I AATT 3 cut(s) 58, 612, 660
SsiI CCGC 5 cut(s) 257, 455, 469, 515, 989
SspMI CTAG 2 cut(s) 320, 1128
StyD4I CCNGG 6 cut(s) 112, 219, 299, 361, 391, 768
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 2 cut(s) 488, 622
TaiI ACGT 7 cut(s) 421, 705, 743, 766, 795, 926, 936
TaqI TCGA 9 cut(s) 293, 328, 494, 535, 546, 561, 624, 782, 1077
TaqII GACCGA 2 cut(s) 149, 175
TasI AATT 3 cut(s) 58, 612, 660
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 2 cut(s) 109, 304
Tru1I TTAA 1 cut(s) 897
Tru9I TTAA 1 cut(s) 897
TscAI CASTG 4 cut(s) 239, 314, 733, 847
TseFI GTSAC 2 cut(s) 130, 724
TseI GCWGC 3 cut(s) 20, 87, 259
Tsp45I GTSAC 2 cut(s) 130, 724
TspDTI ATGAA 1 cut(s) 21
TspGWI ACGGA 1 cut(s) 803
TspRI CASTG 4 cut(s) 239, 314, 733, 847
Van91I CCANNNNNTGG 3 cut(s) 175, 504, 706
VpaK11BI GGWCC 2 cut(s) 471, 507
XapI RAATTY 1 cut(s) 58
XceI RCATGY 3 cut(s) 405, 600, 1020
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 2 cut(s) 320, 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.