Rroxscaffold_7G00163240

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013470
Physical Location & Seq
Reverse (-)
5561013 .. 5562137
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00163240.1

Sequence Viewer

Length: 960 bp
ATGGCAGCTCTGACTTCCTCTGCATCAAACCCAGAAACTGAGAACTCTGACATAGAAAGGAGCAGAAGCAGCAGCAACAAATCTGAGCCGTTGATTCCTGGACTTCCAGACGAAGTGGCCGAGCTGTGTCTTCTTTACCTTCCGTATCCGTACCAAGCTTTGGTGCGCTCGGTCTCTGCTTCGTGGAGGAGAGCCATTTCAGACCCGGCTTTTCTTCACTGCAAGAAGTCACTTTCGCTCCCGCTGCCTTATCTATTCGTTTTCGCCTTCAACAAATCAACGGCCAGGATTCAATGGCAAGCGCTGGACCCTCGATCGGGCCGTTGGTTTGTCCTACCTCCGATGCCGGGGCATTATTCCAAGCTCGCCAGTCCACCGGGGTTAGCGTGTGCCTCCTTGCCACGTCAGGGGCTGCTTGTCGTGTTGGGTGGCTCGGACGCGGAATCGTCTATGCGGTCCACTATTGTATACCATACATCTACCAATCAGTGGTCCAGCATGGCTCCCATGCCGACTGCCCGATCGTATTTTGACGCGGGAAGCATCGATAATAAGATCATAGCGGTCGGAGGTAGTGGGATCCGCGAGAATGACTTGATCAGGGCCGTTGAGTGTTATAACCCTGAAAATGACACATGGGCGACACTGGCCACGATGCCAGCGGGACTTGCCAAGTATGACTCCCACGTGGTGGGGGACAAGATGTACGTCACCGAGGGGTGGACGTGGCCCTTCATGTTCTCGCCACGCGGCCTGATCTATGACTCGAAGACAGACACGTGGCGAGAGATGAGTCCTGGGATGAGGGAAGGGTGGACAGGTGTCAGCGTGGTCGTGGACAACAAGCTGTCTGAATCAGAAGCAGAAAAGGGTGAGCTACAAGTTGAGTGGCAGGTTCTGCCAACTCCAAGTGCTTTTAGGGGTTTTTCTCCTTCAAGTTGCCAAGTGCTTTATGCCTGA

Protein Analysis

319

Amino Acids

35.13

Weight (kDa)

5.67

Isoelectric Point (pI)

59.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 33 - 72 6.4e-06 F-box domain
Beta-prop_Calicin PF13964 108 - 214 4.8e-07 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 127 - 266 5e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 134 - 266 1.1e-07 FBX42, beta-propeller domain
Kelch_1 PF01344 136 - 171 1.6e-07 Kelch motif
Kelch_FKB95 PF25210 141 - 290 3e-13 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 141 - 282 4.2e-09 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 173 - 220 5.8e-09 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24540
fragaria_vesca FvH4_2g36671
malus_domestica MD08G1127900.v1.1 MD15G1107400.v1.1
prunus_persica Prupe.1G459700_v2.0.a1 Prupe.5G016700_v2.0.a1
pyrus_communis pycom08g10690 pycom15g09820
rosa_chinensis RchiOBHm_Chr6g0305031
rosa_laevigata RLG00000010926
rosa_multiflora Rmu_sc0009145.1_g000002
rosa_roxburghii Rroxscaffold_7G00163240
rosa_rugosa Rorug06G0336900
rosa_samantha Rh6AG448500 Rh6BG452400 Rh6CG462200 Rh6DG449100
rosa_wichuraiana Rw6G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
Acc36I ACCTGC 1 cut(s) 883
AccB7I CCANNNNNTGG 3 cut(s) 160, 489, 691
AccI GTMKAC 1 cut(s) 468
AccII CGCG 4 cut(s) 440, 536, 585, 750
AciI CCGC 8 cut(s) 242, 440, 454, 536, 563, 583, 662, 750
AclWI GGATC 2 cut(s) 574, 587
AcoI YGGCCR 3 cut(s) 117, 282, 648
AcvI CACGTG 2 cut(s) 688, 780
AdeI CACNNNGTG 1 cut(s) 691
AfaI GTAC 2 cut(s) 152, 707
AfeI AGCGCT 1 cut(s) 303
AfiI CCNNNNNNNGG 8 cut(s) 160, 316, 317, 347, 407, 489, 691, 720
AflIII ACRYGT 1 cut(s) 777
AgsI TTSAA 3 cut(s) 271, 293, 936
AjiI CACGTC 2 cut(s) 404, 726
AjnI CCWGG 3 cut(s) 97, 284, 796
AluBI AGCT 6 cut(s) 8, 124, 158, 364, 847, 877
AluI AGCT 6 cut(s) 8, 124, 158, 364, 847, 877
Alw26I GTCTC 1 cut(s) 178
AlwI GGATC 2 cut(s) 574, 587
AlwNI CAGNNNCTG 3 cut(s) 38, 412, 898
Aor51HI AGCGCT 1 cut(s) 303
AoxI GGCC 7 cut(s) 117, 282, 319, 603, 648, 728, 751
ApeKI GCWGC 5 cut(s) 5, 69, 72, 244, 412
AspLEI GCGC 2 cut(s) 168, 304
AspS9I GGNCC 6 cut(s) 307, 319, 456, 492, 603, 729
AsuC2I CCSGG 3 cut(s) 206, 348, 378
AsuHPI GGTGA 2 cut(s) 703, 884
AvaII GGWCC 3 cut(s) 307, 456, 492
BalI TGGCCA 1 cut(s) 650
BamHI GGATCC 1 cut(s) 579
BbrPI CACGTG 2 cut(s) 688, 780
BbsI GAAGAC 2 cut(s) 122, 776
BbvI GCAGC 5 cut(s) 17, 81, 84, 231, 399
BceAI ACGGC 4 cut(s) 73, 297, 306, 590
BciT130I CCWGG 3 cut(s) 99, 286, 798
BciVI GTATCC 1 cut(s) 156
BclI TGATCA 1 cut(s) 597
BcnI CCSGG 3 cut(s) 206, 348, 378
BcoDI GTCTC 1 cut(s) 178
BfoI RGCGCY 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 883
BfuI GTATCC 1 cut(s) 156
BisI GCNGC 6 cut(s) 6, 70, 73, 245, 413, 751
BlsI GCNGC 6 cut(s) 7, 71, 74, 246, 414, 752
Bme1390I CCNGG 6 cut(s) 99, 206, 286, 348, 378, 798
Bme18I GGWCC 3 cut(s) 307, 456, 492
BmgBI CACGTC 2 cut(s) 404, 726
BmgT120I GGNCC 6 cut(s) 307, 319, 456, 492, 603, 729
BmiI GGNNCC 3 cut(s) 309, 504, 581
BmrFI CCNGG 6 cut(s) 99, 206, 286, 348, 378, 798
BmsI GCATC 4 cut(s) 32, 333, 552, 645
BoxI GACNNNNGTC 1 cut(s) 821
BpiI GAAGAC 2 cut(s) 122, 776
BpuMI CCSGG 3 cut(s) 206, 348, 378
Bsa29I ATCGAT 1 cut(s) 546
BsaAI YACGTR 2 cut(s) 688, 780
BsaI GGTCTC 1 cut(s) 178
BsaJI CCNNGG 4 cut(s) 347, 377, 714, 797
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 8 cut(s) 160, 316, 317, 347, 407, 489, 691, 720
Bse1I ACTGG 2 cut(s) 369, 651
BseBI CCWGG 3 cut(s) 99, 286, 798
BseCI ATCGAT 1 cut(s) 546
BseDI CCNNGG 4 cut(s) 347, 377, 714, 797
BseGI GGATG 1 cut(s) 807
BseLI CCNNNNNNNGG 8 cut(s) 160, 316, 317, 347, 407, 489, 691, 720
BseMII CTCAG 2 cut(s) 30, 75
BseNI ACTGG 2 cut(s) 369, 651
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 5 cut(s) 17, 81, 84, 231, 399
Bsh1236I CGCG 4 cut(s) 440, 536, 585, 750
Bsh1285I CGRYCG 3 cut(s) 317, 524, 567
BshFI GGCC 7 cut(s) 119, 284, 321, 605, 650, 730, 753
BshVI ATCGAT 1 cut(s) 546
BsiEI CGRYCG 3 cut(s) 317, 524, 567
BsiSI CCGG 3 cut(s) 206, 347, 377
BslFI GGGAC 2 cut(s) 678, 710
BslI CCNNNNNNNGG 8 cut(s) 160, 316, 317, 347, 407, 489, 691, 720
BsmAI GTCTC 1 cut(s) 178
BsmFI GGGAC 2 cut(s) 678, 710
BsnI GGCC 7 cut(s) 119, 284, 321, 605, 650, 730, 753
Bso31I GGTCTC 1 cut(s) 178
Bsp143I GATC 6 cut(s) 314, 521, 555, 579, 597, 756
BspACI CCGC 8 cut(s) 242, 440, 454, 536, 563, 583, 662, 750
BspANI GGCC 7 cut(s) 119, 284, 321, 605, 650, 730, 753
BspCNI CTCAG 2 cut(s) 31, 76
BspDI ATCGAT 1 cut(s) 546
BspFNI CGCG 4 cut(s) 440, 536, 585, 750
BspLI GGNNCC 3 cut(s) 309, 504, 581
BspMI ACCTGC 1 cut(s) 883
BspPI GGATC 2 cut(s) 574, 587
BspTNI GGTCTC 1 cut(s) 178
BsrI ACTGG 2 cut(s) 369, 651
BssECI CCNNGG 4 cut(s) 347, 377, 714, 797
BssMI GATC 6 cut(s) 314, 521, 555, 579, 597, 756
BssNAI GTATAC 1 cut(s) 469
Bst1107I GTATAC 1 cut(s) 469
Bst2UI CCWGG 3 cut(s) 99, 286, 798
BstAPI GCANNNNNTGC 1 cut(s) 898
BstBAI YACGTR 2 cut(s) 688, 780
BstC8I GCNNGC 3 cut(s) 300, 366, 660
BstDEI CTNAG 2 cut(s) 39, 84
BstF5I GGATG 1 cut(s) 807
BstFNI CGCG 4 cut(s) 440, 536, 585, 750
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 2 cut(s) 168, 304
BstKTI GATC 6 cut(s) 317, 524, 558, 582, 600, 759
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 6 cut(s) 314, 521, 555, 579, 597, 756
BstMCI CGRYCG 3 cut(s) 317, 524, 567
BstMWI GCNNNNNNNGC 5 cut(s) 69, 244, 647, 668, 898
BstNI CCWGG 3 cut(s) 99, 286, 798
BstPAI GACNNNNGTC 1 cut(s) 821
BstSCI CCNGG 6 cut(s) 97, 204, 284, 346, 376, 796
BstUI CGCG 4 cut(s) 440, 536, 585, 750
BstV1I GCAGC 5 cut(s) 17, 81, 84, 231, 399
BstV2I GAAGAC 2 cut(s) 122, 776
BstX2I RGATCY 1 cut(s) 579
BstYI RGATCY 1 cut(s) 579
BstZ17I GTATAC 1 cut(s) 469
Bsu15I ATCGAT 1 cut(s) 546
BsuI GTATCC 1 cut(s) 156
BsuRI GGCC 7 cut(s) 119, 284, 321, 605, 650, 730, 753
BsuTUI ATCGAT 1 cut(s) 546
BtrI CACGTC 2 cut(s) 404, 726
BtsCI GGATG 1 cut(s) 807
BtsI GCAGTG 1 cut(s) 217
BtsIMutI CAGTG 3 cut(s) 217, 494, 644
BveI ACCTGC 1 cut(s) 883
Cac8I GCNNGC 3 cut(s) 300, 366, 660
CaiI CAGNNNCTG 3 cut(s) 38, 412, 898
CfoI GCGC 2 cut(s) 168, 304
Cfr13I GGNCC 6 cut(s) 307, 319, 456, 492, 603, 729
ClaI ATCGAT 1 cut(s) 546
CseI GACGC 2 cut(s) 446, 542
Csp6I GTAC 2 cut(s) 151, 706
CspCI CAANNNNNGTGG 2 cut(s) 869, 904
CviAII CATG 4 cut(s) 499, 508, 636, 736
CviQI GTAC 2 cut(s) 151, 706
DdeI CTNAG 2 cut(s) 39, 84
DpnI GATC 6 cut(s) 316, 523, 557, 581, 599, 758
DpnII GATC 6 cut(s) 314, 521, 555, 579, 597, 756
DraIII CACNNNGTG 1 cut(s) 691
EaeI YGGCCR 3 cut(s) 117, 282, 648
Eco31I GGTCTC 1 cut(s) 178
Eco47I GGWCC 3 cut(s) 307, 456, 492
Eco47III AGCGCT 1 cut(s) 303
Eco72I CACGTG 2 cut(s) 688, 780
EcoRII CCWGG 3 cut(s) 97, 284, 796
FaeI CATG 4 cut(s) 502, 511, 639, 739
FaqI GGGAC 2 cut(s) 678, 710
FatI CATG 4 cut(s) 498, 507, 635, 735
FauI CCCGC 3 cut(s) 249, 529, 655
FbaI TGATCA 1 cut(s) 597
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 6 cut(s) 6, 70, 73, 245, 413, 751
FokI GGATG 1 cut(s) 814
Fsp4HI GCNGC 6 cut(s) 6, 70, 73, 245, 413, 751
GlaI GCGC 2 cut(s) 167, 303
GluI GCNGC 6 cut(s) 6, 70, 73, 245, 413, 751
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 7 cut(s) 119, 284, 321, 605, 650, 730, 753
HapII CCGG 3 cut(s) 206, 347, 377
HgaI GACGC 2 cut(s) 446, 542
HhaI GCGC 2 cut(s) 168, 304
Hin1II CATG 4 cut(s) 502, 511, 639, 739
Hin6I GCGC 2 cut(s) 166, 302
HinP1I GCGC 2 cut(s) 166, 302
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 7 cut(s) 94, 289, 443, 680, 764, 793, 854
HpaII CCGG 3 cut(s) 206, 347, 377
HphI GGTGA 2 cut(s) 703, 884
Hpy166II GTNNAC 6 cut(s) 374, 459, 469, 723, 816, 838
Hpy188I TCNGA 9 cut(s) 12, 49, 85, 202, 342, 436, 569, 853, 859
Hpy188III TCNNGA 1 cut(s) 107
Hpy8I GTNNAC 6 cut(s) 374, 459, 469, 723, 816, 838
HpyAV CCTTC 5 cut(s) 149, 277, 742, 803, 942
HpyCH4IV ACGT 5 cut(s) 403, 687, 708, 725, 779
HpyCH4V TGCA 2 cut(s) 23, 222
HpyF10VI GCNNNNNNNGC 5 cut(s) 69, 244, 647, 668, 898
HpyF3I CTNAG 2 cut(s) 39, 84
HpySE526I ACGT 5 cut(s) 403, 687, 708, 725, 779
Hsp92II CATG 4 cut(s) 502, 511, 639, 739
HspAI GCGC 2 cut(s) 166, 302
Ksp22I TGATCA 1 cut(s) 597
Kzo9I GATC 6 cut(s) 314, 521, 555, 579, 597, 756
LmnI GCTCC 3 cut(s) 60, 243, 508
Lsp1109I GCAGC 5 cut(s) 17, 81, 84, 231, 399
LweI GCATC 4 cut(s) 32, 333, 552, 645
MaeII ACGT 5 cut(s) 403, 687, 708, 725, 779
MaeIII GTNAC 2 cut(s) 228, 709
MalI GATC 6 cut(s) 316, 523, 557, 581, 599, 758
MboI GATC 6 cut(s) 314, 521, 555, 579, 597, 756
MboII GAAGA 3 cut(s) 122, 206, 781
MflI RGATCY 1 cut(s) 579
MlsI TGGCCA 1 cut(s) 650
MluNI TGGCCA 1 cut(s) 650
MlyI GAGTC 3 cut(s) 674, 758, 802
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 8 cut(s) 28, 180, 321, 348, 403, 563, 709, 798
Mox20I TGGCCA 1 cut(s) 650
MscI TGGCCA 1 cut(s) 650
Msp20I TGGCCA 1 cut(s) 650
MspA1I CMGCKG 2 cut(s) 244, 662
MspI CCGG 3 cut(s) 206, 347, 377
MspR9I CCNGG 6 cut(s) 99, 206, 286, 348, 378, 798
MvaI CCWGG 3 cut(s) 99, 286, 798
MvnI CGCG 4 cut(s) 440, 536, 585, 750
MwoI GCNNNNNNNGC 5 cut(s) 69, 244, 647, 668, 898
NciI CCSGG 3 cut(s) 206, 348, 378
NdeII GATC 6 cut(s) 314, 521, 555, 579, 597, 756
NlaIII CATG 4 cut(s) 502, 511, 639, 739
NlaIV GGNNCC 3 cut(s) 309, 504, 581
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 2 cut(s) 228, 709
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 319
PfeI GAWTC 4 cut(s) 94, 289, 443, 854
PflMI CCANNNNNTGG 3 cut(s) 160, 489, 691
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 6 cut(s) 7, 71, 74, 246, 414, 752
Ple19I CGATCG 2 cut(s) 317, 524
PleI GAGTC 3 cut(s) 674, 758, 801
PmaCI CACGTG 2 cut(s) 688, 780
PmlI CACGTG 2 cut(s) 688, 780
PpsI GAGTC 3 cut(s) 674, 758, 801
Ppu21I YACGTR 2 cut(s) 688, 780
PshAI GACNNNNGTC 1 cut(s) 821
PsiI TTATAA 1 cut(s) 618
Psp6I CCWGG 3 cut(s) 97, 284, 796
PspCI CACGTG 2 cut(s) 688, 780
PspGI CCWGG 3 cut(s) 97, 284, 796
PspN4I GGNNCC 3 cut(s) 309, 504, 581
PspPI GGNCC 6 cut(s) 307, 319, 456, 492, 603, 729
PstNI CAGNNNCTG 3 cut(s) 38, 412, 898
PsuI RGATCY 1 cut(s) 579
PvuI CGATCG 2 cut(s) 317, 524
RsaI GTAC 2 cut(s) 152, 707
RsaNI GTAC 2 cut(s) 151, 706
SatI GCNGC 6 cut(s) 6, 70, 73, 245, 413, 751
Sau3AI GATC 6 cut(s) 314, 521, 555, 579, 597, 756
Sau96I GGNCC 6 cut(s) 307, 319, 456, 492, 603, 729
SchI GAGTC 3 cut(s) 674, 758, 802
ScrFI CCNGG 6 cut(s) 99, 206, 286, 348, 378, 798
SfaNI GCATC 4 cut(s) 32, 333, 552, 645
SinI GGWCC 3 cut(s) 307, 456, 492
SsiI CCGC 8 cut(s) 242, 440, 454, 536, 563, 583, 662, 750
StyD4I CCNGG 6 cut(s) 97, 204, 284, 346, 376, 796
TaiI ACGT 5 cut(s) 406, 690, 711, 728, 782
TaqI TCGA 3 cut(s) 313, 546, 767
TaqII GACCGA 1 cut(s) 160
TauI GCSGC 1 cut(s) 753
TfiI GAWTC 4 cut(s) 94, 289, 443, 854
TscAI CASTG 3 cut(s) 224, 494, 651
TseFI GTSAC 2 cut(s) 228, 709
TseI GCWGC 5 cut(s) 5, 69, 72, 244, 412
Tsp45I GTSAC 2 cut(s) 228, 709
TspDTI ATGAA 1 cut(s) 724
TspGWI ACGGA 2 cut(s) 132, 138
TspRI CASTG 3 cut(s) 224, 494, 651
Van91I CCANNNNNTGG 3 cut(s) 160, 489, 691
VpaK11BI GGWCC 3 cut(s) 307, 456, 492
XmiI GTMKAC 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.