Prupe.1G459700_v2.0.a1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013470
Physical Location & Seq
Reverse (-)
38472811 .. 38475365
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G459700.1

Sequence Viewer

Length: 1143 bp
ATGGCGGCTGTGAAATCGTCATCTTCATCGGTATCAAACCCAGTTTCTGATCAATGTTCTGACCAAGAAAGCAGCAACAAATCTGAGCCATTAATTCCTGGGTTACCGGACGAGATTGCCGAGCTCTGCCTTCTTTACCTTCCCTACCCCTACCAAGCTCTGGTGCGTTCGGTCTCTGCTTCGTGGAACAGAGCCATCACAGACTCTAGCTTCGTTCGCTGCAAGAAATCTCTGTCTCTTCCTCTGCCTTATCTTTTCGTCTTTGCCTTCAACAAAGTGACGTCCAGGATTCAGTGGCAGGCGCTGGACCCTCGATCGGGCCGTTGGTTTGTTTTACCTCCCATGCCGTTCCCCAAGGCCGCCTGCCCACCGGGCTTCGCATGCACATCGCTGCCACGTCAGGGGAAGCTCGTTGTCTTGGGCGGCGTGCGGTCAGACTCAGAATGTTCTACGCGCACCACCATCGTGTACTGTACGTCTACAAATCAATGGTCAATTGCCGCTCCAATGCGGACCCCACGATCATTTTTTGAGGCGGGAAATATCAATGGTAAGATCTTGGCGGTTGGCGGTGGTGTGGCCCGCAATGGTGATTCGATTAGGGCTGTAGACTGTTACGACCCTGAAAATGACACGTGGGCAGCGTCTGCCACGCTGCCCACGAGTCTAGCCAAGTACGACTCCAATGTGGTGGGAAACAAGATGTATGTCACAGAGGGGTGGATGTGGCCGTTTATGCTTTCGCCACGAGGCGTGGTTTATGACCCAGACGAGGACACTTGGCAGGAGATGAGGCAGGGGATGAGGGACGGGTGGACGGGGGTTAGCGTGGTGGTGGGTGAGAGGTTGTTGGTCATATCGGAGTATGGGGATTGTCCGATGAAGGTGTACGATCCGGATGAGGACACATGGCGGTATGTGAGTGGGGACAAGTTCCCATGTGAGGCACTGAGAAGGCCTTTTGCTGCGAGCGGGGTGGAAGGGAACATATATGTGGTGGCATGTGGGTTGAATGTGGGAATAGGAAGGCTGTCTGAGTGTGGAGGAGGAAAGGATGAGCTAAAAGTTGAGTGGCAGGTTGTGCCAGCTCCAAGTGCTTTTAGGGGCTTCTCTCCTTCTAGTTGTCAAGTACTTTACGCCTAA

Protein Analysis

381

Amino Acids

41.58

Weight (kDa)

5.35

Isoelectric Point (pI)

54.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24540
fragaria_vesca FvH4_2g36671
malus_domestica MD08G1127900.v1.1 MD15G1107400.v1.1
prunus_persica Prupe.1G459700_v2.0.a1 Prupe.5G016700_v2.0.a1
pyrus_communis pycom08g10690 pycom15g09820
rosa_chinensis RchiOBHm_Chr6g0305031
rosa_laevigata RLG00000010926
rosa_multiflora Rmu_sc0009145.1_g000002
rosa_roxburghii Rroxscaffold_7G00163240
rosa_rugosa Rorug06G0336900
rosa_samantha Rh6AG448500 Rh6BG452400 Rh6CG462200 Rh6DG449100
rosa_wichuraiana Rw6G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 1066
AccB7I CCANNNNNTGG 1 cut(s) 160
AccBSI CCGCTC 2 cut(s) 503, 972
AccI GTMKAC 2 cut(s) 479, 609
AccII CGCG 1 cut(s) 454
AccIII TCCGGA 1 cut(s) 895
AclWI GGATC 1 cut(s) 887
AcoI YGGCCR 1 cut(s) 728
AcvI CACGTG 1 cut(s) 636
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 5 cut(s) 470, 475, 677, 890, 1131
AfiI CCNNNNNNNGG 6 cut(s) 160, 316, 317, 401, 772, 943
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 2 cut(s) 271, 1012
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 2 cut(s) 97, 284
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 6 cut(s) 124, 158, 210, 409, 1060, 1088
AluI AGCT 6 cut(s) 124, 158, 210, 409, 1060, 1088
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 178, 240
AlwI GGATC 1 cut(s) 887
AlwNI CAGNNNCTG 3 cut(s) 47, 304, 647
Aor13HI TCCGGA 1 cut(s) 895
AoxI GGCC 5 cut(s) 319, 357, 579, 728, 956
ApeKI GCWGC 6 cut(s) 72, 219, 391, 641, 655, 965
ArsI GACNNNNNNTTYG 4 cut(s) 194, 226, 476, 508
AseI ATTAAT 1 cut(s) 92
AspLEI GCGC 2 cut(s) 304, 456
AspS9I GGNCC 4 cut(s) 307, 319, 513, 580
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 2 cut(s) 602, 851
AvaII GGWCC 2 cut(s) 307, 513
BanII GRGCYC 1 cut(s) 126
BauI CACGAG 2 cut(s) 661, 747
BbrPI CACGTG 1 cut(s) 636
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 6 cut(s) 84, 206, 378, 642, 653, 952
BccI CCATC 2 cut(s) 203, 470
BceAI ACGGC 3 cut(s) 306, 331, 715
BcgI CGANNNNNNTGC 4 cut(s) 369, 403, 445, 479
BciT130I CCWGG 2 cut(s) 99, 286
BclI TGATCA 1 cut(s) 49
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 2 cut(s) 178, 240
BfaI CTAG 3 cut(s) 207, 668, 1119
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 1066
BglI GCCNNNNNGGC 1 cut(s) 372
BglII AGATCT 1 cut(s) 555
BmcAI AGTACT 1 cut(s) 1131
Bme1390I CCNGG 3 cut(s) 99, 286, 372
Bme18I GGWCC 2 cut(s) 307, 513
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 4 cut(s) 307, 319, 513, 580
BmiI GGNNCC 2 cut(s) 309, 515
BmrFI CCNGG 3 cut(s) 99, 286, 372
BmrI ACTGGG 1 cut(s) 35
BmuI ACTGGG 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 372
BsaAI YACGTR 1 cut(s) 636
BsaHI GRCGYC 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 178
BsaJI CCNNGG 2 cut(s) 98, 354
BsaWI WCCGGW 2 cut(s) 106, 895
Bsc4I CCNNNNNNNGG 6 cut(s) 160, 316, 317, 401, 772, 943
Bse1I ACTGG 1 cut(s) 41
Bse3DI GCAATG 1 cut(s) 592
BseAI TCCGGA 1 cut(s) 895
BseBI CCWGG 2 cut(s) 99, 286
BseDI CCNNGG 2 cut(s) 98, 354
BseGI GGATG 4 cut(s) 729, 807, 904, 1060
BseLI CCNNNNNNNGG 6 cut(s) 160, 316, 317, 401, 772, 943
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 4 cut(s) 75, 453, 941, 1026
BseNI ACTGG 1 cut(s) 41
BseRI GAGGAG 1 cut(s) 1059
BseXI GCAGC 6 cut(s) 84, 206, 378, 642, 653, 952
Bsh1236I CGCG 1 cut(s) 454
Bsh1285I CGRYCG 1 cut(s) 317
BshFI GGCC 5 cut(s) 321, 359, 581, 730, 958
BsiEI CGRYCG 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 3 cut(s) 107, 371, 896
BslFI GGGAC 2 cut(s) 821, 941
BslI CCNNNNNNNGG 6 cut(s) 160, 316, 317, 401, 772, 943
BsmAI GTCTC 2 cut(s) 178, 240
BsmFI GGGAC 2 cut(s) 821, 941
BsnI GGCC 5 cut(s) 321, 359, 581, 730, 958
Bso31I GGTCTC 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 126
Bsp13I TCCGGA 1 cut(s) 895
Bsp143I GATC 5 cut(s) 49, 314, 521, 555, 892
BspANI GGCC 5 cut(s) 321, 359, 581, 730, 958
BspCNI CTCAG 4 cut(s) 76, 452, 942, 1027
BspEI TCCGGA 1 cut(s) 895
BspFNI CGCG 1 cut(s) 454
BspLI GGNNCC 2 cut(s) 309, 515
BspMI ACCTGC 1 cut(s) 1066
BspPI GGATC 1 cut(s) 887
BspTNI GGTCTC 1 cut(s) 178
BsrBI CCGCTC 2 cut(s) 503, 972
BsrDI GCAATG 1 cut(s) 592
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 98, 354
BssMI GATC 5 cut(s) 49, 314, 521, 555, 892
BssNI GRCGYC 1 cut(s) 281
BssSI CACGAG 2 cut(s) 661, 747
BssT1I CCWWGG 1 cut(s) 354
Bst2BI CACGAG 2 cut(s) 661, 747
Bst2UI CCWGG 2 cut(s) 99, 286
Bst4CI ACNGT 2 cut(s) 473, 614
Bst6I CTCTTC 1 cut(s) 243
BstACI GRCGYC 1 cut(s) 281
BstAPI GCANNNNNTGC 2 cut(s) 647, 1081
BstBAI YACGTR 1 cut(s) 636
BstC8I GCNNGC 7 cut(s) 300, 364, 382, 428, 583, 970, 1086
BstDEI CTNAG 4 cut(s) 84, 439, 950, 1035
BstEII GGTNACC 1 cut(s) 102
BstF5I GGATG 4 cut(s) 729, 807, 904, 1060
BstFNI CGCG 1 cut(s) 454
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 2 cut(s) 304, 456
BstKTI GATC 5 cut(s) 52, 317, 524, 558, 895
BstMAI GTCTC 2 cut(s) 178, 240
BstMBI GATC 5 cut(s) 49, 314, 521, 555, 892
BstMCI CGRYCG 1 cut(s) 317
BstMWI GCNNNNNNNGC 7 cut(s) 216, 372, 381, 647, 736, 1081, 1094
BstNI CCWGG 2 cut(s) 99, 286
BstNSI RCATGY 2 cut(s) 384, 1005
BstPI GGTNACC 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 97, 284, 370
BstSFI CTRYAG 1 cut(s) 606
BstUI CGCG 1 cut(s) 454
BstV1I GCAGC 6 cut(s) 84, 206, 378, 642, 653, 952
BstX2I RGATCY 1 cut(s) 555
BstXI CCANNNNNNTGG 1 cut(s) 691
BstYI RGATCY 1 cut(s) 555
BsuRI GGCC 5 cut(s) 321, 359, 581, 730, 958
BtgZI GCGATG 1 cut(s) 372
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 4 cut(s) 729, 807, 904, 1060
BtsIMutI CAGTG 2 cut(s) 299, 947
BveI ACCTGC 1 cut(s) 1066
Cac8I GCNNGC 7 cut(s) 300, 364, 382, 428, 583, 970, 1086
CaiI CAGNNNCTG 3 cut(s) 47, 304, 647
CfoI GCGC 2 cut(s) 304, 456
Cfr13I GGNCC 4 cut(s) 307, 319, 513, 580
CseI GACGC 1 cut(s) 633
Csp6I GTAC 5 cut(s) 469, 474, 676, 889, 1130
CviAII CATG 5 cut(s) 343, 381, 909, 939, 1002
CviQI GTAC 5 cut(s) 469, 474, 676, 889, 1130
DdeI CTNAG 4 cut(s) 84, 439, 950, 1035
DpnI GATC 5 cut(s) 51, 316, 523, 557, 894
DpnII GATC 5 cut(s) 49, 314, 521, 555, 892
EaeI YGGCCR 1 cut(s) 728
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 354
Eco147I AGGCCT 1 cut(s) 958
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 178
Eco47I GGWCC 2 cut(s) 307, 513
Eco53kI GAGCTC 1 cut(s) 124
Eco72I CACGTG 1 cut(s) 636
Eco91I GGTNACC 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 124
EcoO65I GGTNACC 1 cut(s) 102
EcoRII CCWGG 2 cut(s) 97, 284
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 5 cut(s) 346, 384, 912, 942, 1005
FaqI GGGAC 2 cut(s) 821, 941
FatI CATG 5 cut(s) 342, 380, 908, 938, 1001
FauI CCCGC 3 cut(s) 529, 590, 965
FbaI TGATCA 1 cut(s) 49
FblI GTMKAC 2 cut(s) 479, 609
FokI GGATG 4 cut(s) 736, 814, 911, 1067
FriOI GRGCYC 1 cut(s) 126
FspBI CTAG 3 cut(s) 207, 668, 1119
GlaI GCGC 2 cut(s) 303, 455
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 5 cut(s) 321, 359, 581, 730, 958
HapII CCGG 3 cut(s) 107, 371, 896
HgaI GACGC 1 cut(s) 633
HhaI GCGC 2 cut(s) 304, 456
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 5 cut(s) 346, 384, 912, 942, 1005
Hin6I GCGC 2 cut(s) 302, 454
HinP1I GCGC 2 cut(s) 302, 454
HinfI GANTC 6 cut(s) 203, 289, 437, 593, 664, 680
HpaII CCGG 3 cut(s) 107, 371, 896
HphI GGTGA 2 cut(s) 602, 851
Hpy166II GTNNAC 5 cut(s) 469, 480, 610, 816, 889
Hpy188I TCNGA 8 cut(s) 49, 61, 85, 436, 442, 862, 879, 1036
Hpy188III TCNNGA 1 cut(s) 896
Hpy8I GTNNAC 5 cut(s) 469, 480, 610, 816, 889
HpyAV CCTTC 8 cut(s) 140, 149, 277, 877, 948, 974, 1020, 1125
HpyCH4III ACNGT 2 cut(s) 473, 614
HpyCH4IV ACGT 4 cut(s) 281, 397, 476, 635
HpyCH4V TGCA 2 cut(s) 222, 384
HpyF10VI GCNNNNNNNGC 7 cut(s) 216, 372, 381, 647, 736, 1081, 1094
HpyF3I CTNAG 4 cut(s) 84, 439, 950, 1035
HpySE526I ACGT 4 cut(s) 281, 397, 476, 635
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 5 cut(s) 346, 384, 912, 942, 1005
HspAI GCGC 2 cut(s) 302, 454
Kpn2I TCCGGA 1 cut(s) 895
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 5 cut(s) 49, 314, 521, 555, 892
LmnI GCTCC 2 cut(s) 508, 1093
Lsp1109I GCAGC 6 cut(s) 84, 206, 378, 642, 653, 952
MaeI CTAG 3 cut(s) 207, 668, 1119
MaeII ACGT 4 cut(s) 281, 397, 476, 635
MaeIII GTNAC 4 cut(s) 102, 277, 614, 709
MalI GATC 5 cut(s) 51, 316, 523, 557, 894
MbiI CCGCTC 2 cut(s) 503, 972
MboI GATC 5 cut(s) 49, 314, 521, 555, 892
MboII GAAGA 2 cut(s) 15, 230
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 555
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 2 cut(s) 93, 495
MlyI GAGTC 4 cut(s) 197, 431, 673, 674
MroI TCCGGA 1 cut(s) 895
MseI TTAA 1 cut(s) 92
MslI CAYNNNNRTG 2 cut(s) 464, 992
MspI CCGG 3 cut(s) 107, 371, 896
MspR9I CCNGG 3 cut(s) 99, 286, 372
MunI CAATTG 1 cut(s) 495
MvaI CCWGG 2 cut(s) 99, 286
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 7 cut(s) 216, 372, 381, 647, 736, 1081, 1094
NciI CCSGG 1 cut(s) 372
NdeII GATC 5 cut(s) 49, 314, 521, 555, 892
NlaIII CATG 5 cut(s) 346, 384, 912, 942, 1005
NlaIV GGNNCC 2 cut(s) 309, 515
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 2 cut(s) 277, 709
NspI RCATGY 2 cut(s) 384, 1005
OliI CACNNNNGTG 1 cut(s) 464
PaeI GCATGC 1 cut(s) 384
PceI AGGCCT 1 cut(s) 958
PcsI WCGNNNNNNNCGW 4 cut(s) 117, 319, 641, 659
PfeI GAWTC 2 cut(s) 289, 593
PflMI CCANNNNNTGG 1 cut(s) 160
PfoI TCCNGGA 1 cut(s) 284
Ple19I CGATCG 1 cut(s) 317
PleI GAGTC 4 cut(s) 197, 431, 672, 674
PmaCI CACGTG 1 cut(s) 636
PmlI CACGTG 1 cut(s) 636
PpsI GAGTC 4 cut(s) 197, 431, 672, 674
Ppu21I YACGTR 1 cut(s) 636
PshBI ATTAAT 1 cut(s) 92
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 97, 284
PspCI CACGTG 1 cut(s) 636
PspEI GGTNACC 1 cut(s) 102
PspGI CCWGG 2 cut(s) 97, 284
PspN4I GGNNCC 2 cut(s) 309, 515
PspPI GGNCC 4 cut(s) 307, 319, 513, 580
PstNI CAGNNNCTG 3 cut(s) 47, 304, 647
PsuI RGATCY 1 cut(s) 555
PvuI CGATCG 1 cut(s) 317
RsaI GTAC 5 cut(s) 470, 475, 677, 890, 1131
RsaNI GTAC 5 cut(s) 469, 474, 676, 889, 1130
RseI CAYNNNNRTG 2 cut(s) 464, 992
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 92
Sau3AI GATC 5 cut(s) 49, 314, 521, 555, 892
Sau96I GGNCC 4 cut(s) 307, 319, 513, 580
ScaI AGTACT 1 cut(s) 1131
SchI GAGTC 4 cut(s) 197, 431, 673, 674
ScrFI CCNGG 3 cut(s) 99, 286, 372
SduI GDGCHC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 606
SinI GGWCC 2 cut(s) 307, 513
SmiMI CAYNNNNRTG 2 cut(s) 464, 992
SphI GCATGC 1 cut(s) 384
Sse9I AATT 2 cut(s) 93, 495
SseBI AGGCCT 1 cut(s) 958
SspMI CTAG 3 cut(s) 207, 668, 1119
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 1 cut(s) 958
StyD4I CCNGG 3 cut(s) 97, 284, 370
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 473, 614
TaiI ACGT 4 cut(s) 284, 400, 479, 638
TaqI TCGA 2 cut(s) 313, 596
TaqII GACCGA 1 cut(s) 160
TasI AATT 2 cut(s) 93, 495
TatI WGTACW 2 cut(s) 468, 1129
TauI GCSGC 4 cut(s) 8, 362, 426, 503
TfiI GAWTC 2 cut(s) 289, 593
Tru1I TTAA 1 cut(s) 92
Tru9I TTAA 1 cut(s) 92
TscAI CASTG 2 cut(s) 299, 954
TseFI GTSAC 2 cut(s) 277, 709
TseI GCWGC 6 cut(s) 72, 219, 391, 641, 655, 965
Tsp45I GTSAC 2 cut(s) 277, 709
TspDTI ATGAA 2 cut(s) 15, 896
TspRI CASTG 2 cut(s) 299, 954
Van91I CCANNNNNTGG 1 cut(s) 160
VpaK11BI GGWCC 2 cut(s) 307, 513
VspI ATTAAT 1 cut(s) 92
XceI RCATGY 2 cut(s) 384, 1005
XmiI GTMKAC 2 cut(s) 479, 609
XspI CTAG 3 cut(s) 207, 668, 1119
ZraI GACGTC 1 cut(s) 282
ZrmI AGTACT 1 cut(s) 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.