pycom15g09820

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013470
Physical Location & Seq
Reverse (-)
6389606 .. 6390736
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g09820.1

Sequence Viewer

Length: 1131 bp
ATGGCGGCTCCGAGAACGTCGTCTGCATCAAACCCAGGTTCTGATCAGTGTTCTAAACAAGAAATCAGAATGAGGAAATCTGAGCCGTTGATTCCCGGATTGCCGGATGAGGTTGCTGAGCTCTGCCTGCTCTATCTTCCATATCCGTACCAGGCTCTGGTGCGTTCGGTCTCTGCTTCATGGAAAAGAGCCATTACAGACCCTAGCTTTGTTCTTTGCAAGAAGTCCTTGTGTTTGCCTTTGCCTTATCTTTTCGTTTTCGCCTTCAACAAATCAACGGCTAGGATGCAGTGGCAAGCGTTGGATCCGCGATCTGGCCGTTGGTTTGTTTTGCCTCCCATGCCGTGTCCCAAGGCCGCTTGCCCGCCGGGTTTCGCATGCGCTTCCCTGCCACGTGAGGGGACGCTCGTTGTATTGGGCGGCGTGCGGGCAGACAGGGAATCCTCTACGAAGACAACCATCGTGTATCGTACATCTACCAATCAATGGTCAACAGCTACCCCAATGTCGACCCCGCGGTCATTTTTCGACGCGGGAAATATCAATGGAAAGATCTTGGCCGTTGGAGGCGACAAGGCTGGCTATGGCAGTTCGATCAGGGCGGTAGACTGTTACGACCCGCTGAAAGACACGTGGGCGCCATGTGCCACCCTGCCAGCTGGATTGGCCAAGTATGACTCCAACGTGGTGGGGAACAAGATGTACGTGACCGAGGGGTGGACGTGGCCGTTTATGCACTCGCCACGTGGTGTGGTCTACGACCCAGACAATGACACGTGGCAGGAGATGAGGCCGGGAATGAGGGACGGGTGGACGGGCGTGAGCGTGGTCGTGGGCGGCAGGCTCTTGGTGATATCGGAGTATGGGGACTGTCCGATTAAGGTGTACGACCCTGATAAGGACACGTGGCAGTACGTGGGTGGGGACAGATTCCCATGTGAGGCACTGAGACGTCCCTTTGCTGCGAGTGGGGTGGAAGGGAACATATGCGTGGTGGCATGTGGGTTGAATGTGGGAATCGGAAGGCTGTCCGAGTGGGAAGCAGAGCTAAAAGTTGAGTGGCAGCTTCTGCCAGCTCCGAGTGCTTTTAGGGGATTTTCTCCTTCTAGTTGTCAACTTCTTTTCGCCTAA

Protein Analysis

377

Amino Acids

41.18

Weight (kDa)

8.33

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 98 - 265 1.5e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 107 - 219 5.7e-08 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 134 - 270 7.7e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 139 - 308 5.5e-15 FKB95, Kelch-repeats domain
Kelch_1 PF01344 172 - 219 1.5e-08 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24540
fragaria_vesca FvH4_2g36671
malus_domestica MD08G1127900.v1.1 MD15G1107400.v1.1
prunus_persica Prupe.1G459700_v2.0.a1 Prupe.5G016700_v2.0.a1
pyrus_communis pycom08g10690 pycom15g09820
rosa_chinensis RchiOBHm_Chr6g0305031
rosa_laevigata RLG00000010926
rosa_multiflora Rmu_sc0009145.1_g000002
rosa_roxburghii Rroxscaffold_7G00163240
rosa_rugosa Rorug06G0336900
rosa_samantha Rh6AG448500 Rh6BG452400 Rh6CG462200 Rh6DG449100
rosa_wichuraiana Rw6G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 517
AatII GACGTC 1 cut(s) 955
AccB1I GGYRCC 1 cut(s) 637
AccB7I CCANNNNNTGG 2 cut(s) 157, 486
AccI GTMKAC 3 cut(s) 509, 606, 756
AccII CGCG 3 cut(s) 310, 517, 533
AclWI GGATC 2 cut(s) 299, 312
AcoI YGGCCR 4 cut(s) 316, 558, 666, 725
AcvI CACGTG 5 cut(s) 395, 633, 746, 777, 906
AcyI GRCGYC 2 cut(s) 638, 952
AdeI CACNNNGTG 1 cut(s) 749
AfaI GTAC 5 cut(s) 149, 472, 704, 887, 914
AfiI CCNNNNNNNGG 7 cut(s) 157, 314, 398, 486, 717, 898, 940
AflIII ACRYGT 3 cut(s) 630, 774, 903
AgsI TTSAA 2 cut(s) 268, 1009
AjiI CACGTC 1 cut(s) 723
AjnI CCWGG 2 cut(s) 34, 150
AluBI AGCT 7 cut(s) 121, 207, 497, 659, 1048, 1066, 1076
AluI AGCT 7 cut(s) 121, 207, 497, 659, 1048, 1066, 1076
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 2 cut(s) 175, 943
AlwI GGATC 2 cut(s) 299, 312
AlwNI CAGNNNCTG 3 cut(s) 41, 157, 1069
AoxI GGCC 6 cut(s) 316, 354, 558, 666, 725, 791
ApeKI GCWGC 2 cut(s) 962, 1063
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspLEI GCGC 2 cut(s) 383, 640
AsuC2I CCSGG 3 cut(s) 96, 369, 795
AsuHPI GGTGA 1 cut(s) 862
BalI TGGCCA 1 cut(s) 668
BamHI GGATCC 1 cut(s) 304
BanI GGYRCC 1 cut(s) 637
BanII GRGCYC 1 cut(s) 123
BbrPI CACGTG 5 cut(s) 395, 633, 746, 777, 906
BbsI GAAGAC 1 cut(s) 458
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 949, 1075
BccI CCATC 1 cut(s) 467
BceAI ACGGC 6 cut(s) 70, 294, 303, 328, 545, 712
BciT130I CCWGG 2 cut(s) 36, 152
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 3 cut(s) 96, 369, 795
BcoDI GTCTC 2 cut(s) 175, 943
BfaI CTAG 3 cut(s) 204, 282, 1107
BfoI RGCGCY 1 cut(s) 641
BglII AGATCT 1 cut(s) 552
BisI GCNGC 6 cut(s) 6, 357, 421, 838, 963, 1064
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 6 cut(s) 7, 358, 422, 839, 964, 1065
Bme1390I CCNGG 5 cut(s) 36, 96, 152, 369, 795
BmgBI CACGTC 1 cut(s) 723
BmiI GGNNCC 3 cut(s) 9, 306, 639
BmrFI CCNGG 5 cut(s) 36, 96, 152, 369, 795
BmsI GCATC 2 cut(s) 35, 276
BpiI GAAGAC 1 cut(s) 458
Bpu1102I GCTNAGC 1 cut(s) 117
BpuMI CCSGG 3 cut(s) 96, 369, 795
BsaAI YACGTR 7 cut(s) 395, 633, 706, 746, 777, 906, 916
BsaHI GRCGYC 2 cut(s) 638, 952
BsaI GGTCTC 1 cut(s) 175
BsaJI CCNNGG 4 cut(s) 34, 351, 515, 711
Bsc4I CCNNNNNNNGG 7 cut(s) 157, 314, 398, 486, 717, 898, 940
BseBI CCWGG 2 cut(s) 36, 152
BseDI CCNNGG 4 cut(s) 34, 351, 515, 711
BseGI GGATG 2 cut(s) 112, 291
BseLI CCNNNNNNNGG 7 cut(s) 157, 314, 398, 486, 717, 898, 940
BseMII CTCAG 3 cut(s) 72, 108, 938
BseXI GCAGC 2 cut(s) 949, 1075
Bsh1236I CGCG 3 cut(s) 310, 517, 533
BshFI GGCC 6 cut(s) 318, 356, 560, 668, 727, 793
BshNI GGYRCC 1 cut(s) 637
BsiHKAI GWGCWC 1 cut(s) 123
BsiSI CCGG 4 cut(s) 96, 104, 368, 794
BslFI GGGAC 6 cut(s) 333, 415, 818, 881, 938, 939
BslI CCNNNNNNNGG 7 cut(s) 157, 314, 398, 486, 717, 898, 940
BsmAI GTCTC 2 cut(s) 175, 943
BsmBI CGTCTC 1 cut(s) 943
BsmFI GGGAC 6 cut(s) 333, 415, 818, 881, 938, 939
BsnI GGCC 6 cut(s) 318, 356, 560, 668, 727, 793
Bso31I GGTCTC 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 5 cut(s) 43, 304, 311, 552, 594
Bsp1720I GCTNAGC 1 cut(s) 117
BspANI GGCC 6 cut(s) 318, 356, 560, 668, 727, 793
BspCNI CTCAG 3 cut(s) 73, 109, 939
BspFNI CGCG 3 cut(s) 310, 517, 533
BspLI GGNNCC 3 cut(s) 9, 306, 639
BspPI GGATC 2 cut(s) 299, 312
BspT107I GGYRCC 1 cut(s) 637
BspTNI GGTCTC 1 cut(s) 175
BssECI CCNNGG 4 cut(s) 34, 351, 515, 711
BssMI GATC 5 cut(s) 43, 304, 311, 552, 594
BssNI GRCGYC 2 cut(s) 638, 952
BssT1I CCWWGG 1 cut(s) 351
Bst2UI CCWGG 2 cut(s) 36, 152
Bst4CI ACNGT 2 cut(s) 611, 872
BstACI GRCGYC 2 cut(s) 638, 952
BstAPI GCANNNNNTGC 1 cut(s) 1069
BstBAI YACGTR 7 cut(s) 395, 633, 706, 746, 777, 906, 916
BstDEI CTNAG 3 cut(s) 81, 117, 947
BstDSI CCRYGG 1 cut(s) 515
BstF5I GGATG 2 cut(s) 112, 291
BstFNI CGCG 3 cut(s) 310, 517, 533
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 2 cut(s) 383, 640
BstKTI GATC 5 cut(s) 46, 307, 314, 555, 597
BstMAI GTCTC 2 cut(s) 175, 943
BstMBI GATC 5 cut(s) 43, 304, 311, 552, 594
BstMWI GCNNNNNNNGC 7 cut(s) 127, 340, 644, 665, 733, 1069, 1082
BstNI CCWGG 2 cut(s) 36, 152
BstNSI RCATGY 2 cut(s) 381, 1002
BstSCI CCNGG 5 cut(s) 34, 94, 150, 367, 793
BstUI CGCG 3 cut(s) 310, 517, 533
BstV1I GCAGC 2 cut(s) 949, 1075
BstV2I GAAGAC 1 cut(s) 458
BstX2I RGATCY 2 cut(s) 304, 552
BstXI CCANNNNNNTGG 1 cut(s) 688
BstYI RGATCY 2 cut(s) 304, 552
BsuRI GGCC 6 cut(s) 318, 356, 560, 668, 727, 793
BtgI CCRYGG 1 cut(s) 515
BtrI CACGTC 1 cut(s) 723
BtsCI GGATG 2 cut(s) 112, 291
BtsI GCAGTG 1 cut(s) 296
BtsIMutI CAGTG 3 cut(s) 53, 296, 944
CaiI CAGNNNCTG 3 cut(s) 41, 157, 1069
CfoI GCGC 2 cut(s) 383, 640
Cfr42I CCGCGG 1 cut(s) 518
CseI GACGC 2 cut(s) 412, 539
Csp6I GTAC 5 cut(s) 148, 471, 703, 886, 913
CviAII CATG 6 cut(s) 180, 340, 378, 642, 936, 999
CviQI GTAC 5 cut(s) 148, 471, 703, 886, 913
DdeI CTNAG 3 cut(s) 81, 117, 947
DinI GGCGCC 1 cut(s) 639
DpnI GATC 5 cut(s) 45, 306, 313, 554, 596
DpnII GATC 5 cut(s) 43, 304, 311, 552, 594
DraIII CACNNNGTG 1 cut(s) 749
DrdI GACNNNNNNGTC 1 cut(s) 517
DseDI GACNNNNNNGTC 1 cut(s) 517
EaeI YGGCCR 4 cut(s) 316, 558, 666, 725
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 175
Eco32I GATATC 1 cut(s) 855
Eco53kI GAGCTC 1 cut(s) 121
Eco72I CACGTG 5 cut(s) 395, 633, 746, 777, 906
EcoICRI GAGCTC 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 34, 150
EcoRV GATATC 1 cut(s) 855
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 123
EgeI GGCGCC 1 cut(s) 639
EheI GGCGCC 1 cut(s) 639
ErhI CCWWGG 1 cut(s) 351
Esp3I CGTCTC 1 cut(s) 943
FaeI CATG 6 cut(s) 183, 343, 381, 645, 939, 1002
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FaqI GGGAC 6 cut(s) 333, 415, 818, 881, 938, 939
FatI CATG 6 cut(s) 179, 339, 377, 641, 935, 998
FauI CCCGC 5 cut(s) 372, 420, 522, 526, 627
FauNDI CATATG 1 cut(s) 986
FbaI TGATCA 1 cut(s) 43
FblI GTMKAC 3 cut(s) 509, 606, 756
Fnu4HI GCNGC 6 cut(s) 6, 357, 421, 838, 963, 1064
FokI GGATG 2 cut(s) 119, 298
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 6 cut(s) 6, 357, 421, 838, 963, 1064
FspBI CTAG 3 cut(s) 204, 282, 1107
GlaI GCGC 2 cut(s) 382, 639
GluI GCNGC 6 cut(s) 6, 357, 421, 838, 963, 1064
HaeII RGCGCY 1 cut(s) 641
HaeIII GGCC 6 cut(s) 318, 356, 560, 668, 727, 793
HapII CCGG 4 cut(s) 96, 104, 368, 794
HgaI GACGC 2 cut(s) 412, 539
HhaI GCGC 2 cut(s) 383, 640
Hin1I GRCGYC 2 cut(s) 638, 952
Hin1II CATG 6 cut(s) 183, 343, 381, 645, 939, 1002
Hin6I GCGC 2 cut(s) 381, 638
HinP1I GCGC 2 cut(s) 381, 638
HincII GTYRAC 3 cut(s) 492, 510, 1115
HindII GTYRAC 3 cut(s) 492, 510, 1115
HinfI GANTC 5 cut(s) 91, 440, 677, 930, 1017
HpaII CCGG 4 cut(s) 96, 104, 368, 794
HphI GGTGA 1 cut(s) 862
Hpy166II GTNNAC 8 cut(s) 492, 510, 607, 720, 757, 813, 886, 1115
Hpy188I TCNGA 9 cut(s) 12, 43, 68, 82, 859, 876, 1022, 1033, 1080
Hpy8I GTNNAC 8 cut(s) 492, 510, 607, 720, 757, 813, 886, 1115
Hpy99I CGWCG 2 cut(s) 22, 533
HpyAV CCTTC 4 cut(s) 274, 971, 1017, 1113
HpyCH4III ACNGT 2 cut(s) 611, 872
HpyCH4V TGCA 4 cut(s) 26, 219, 289, 736
HpyF10VI GCNNNNNNNGC 7 cut(s) 127, 340, 644, 665, 733, 1069, 1082
HpyF3I CTNAG 3 cut(s) 81, 117, 947
Hsp92I GRCGYC 2 cut(s) 638, 952
Hsp92II CATG 6 cut(s) 183, 343, 381, 645, 939, 1002
HspAI GCGC 2 cut(s) 381, 638
KasI GGCGCC 1 cut(s) 637
Ksp22I TGATCA 1 cut(s) 43
KspI CCGCGG 1 cut(s) 518
Kzo9I GATC 5 cut(s) 43, 304, 311, 552, 594
LmnI GCTCC 2 cut(s) 13, 1081
Lsp1109I GCAGC 2 cut(s) 949, 1075
LweI GCATC 2 cut(s) 35, 276
MaeI CTAG 3 cut(s) 204, 282, 1107
MaeIII GTNAC 2 cut(s) 611, 706
MalI GATC 5 cut(s) 45, 306, 313, 554, 596
MboI GATC 5 cut(s) 43, 304, 311, 552, 594
MboII GAAGA 2 cut(s) 128, 463
MflI RGATCY 2 cut(s) 304, 552
MhlI GDGCHC 1 cut(s) 123
MlsI TGGCCA 1 cut(s) 668
MluNI TGGCCA 1 cut(s) 668
Mly113I GGCGCC 1 cut(s) 638
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 3 cut(s) 282, 544, 705
Mox20I TGGCCA 1 cut(s) 668
MscI TGGCCA 1 cut(s) 668
MseI TTAA 1 cut(s) 879
MslI CAYNNNNRTG 1 cut(s) 989
Msp20I TGGCCA 1 cut(s) 668
MspA1I CMGCKG 3 cut(s) 517, 622, 659
MspI CCGG 4 cut(s) 96, 104, 368, 794
MspR9I CCNGG 5 cut(s) 36, 96, 152, 369, 795
MvaI CCWGG 2 cut(s) 36, 152
MvnI CGCG 3 cut(s) 310, 517, 533
MwoI GCNNNNNNNGC 7 cut(s) 127, 340, 644, 665, 733, 1069, 1082
NarI GGCGCC 1 cut(s) 638
NciI CCSGG 3 cut(s) 96, 369, 795
NdeI CATATG 1 cut(s) 986
NdeII GATC 5 cut(s) 43, 304, 311, 552, 594
NlaIII CATG 6 cut(s) 183, 343, 381, 645, 939, 1002
NlaIV GGNNCC 3 cut(s) 9, 306, 639
NmuCI GTSAC 1 cut(s) 706
NspI RCATGY 2 cut(s) 381, 1002
PaeI GCATGC 1 cut(s) 381
PfeI GAWTC 4 cut(s) 91, 440, 930, 1017
PflMI CCANNNNNTGG 2 cut(s) 157, 486
PfoI TCCNGGA 1 cut(s) 94
PkrI GCNGC 6 cut(s) 7, 358, 422, 839, 964, 1065
PleI GAGTC 1 cut(s) 671
PluTI GGCGCC 1 cut(s) 641
PmaCI CACGTG 5 cut(s) 395, 633, 746, 777, 906
PmlI CACGTG 5 cut(s) 395, 633, 746, 777, 906
PpsI GAGTC 1 cut(s) 671
Ppu21I YACGTR 7 cut(s) 395, 633, 706, 746, 777, 906, 916
Psp124BI GAGCTC 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 34, 150
PspCI CACGTG 5 cut(s) 395, 633, 746, 777, 906
PspGI CCWGG 2 cut(s) 34, 150
PspN4I GGNNCC 3 cut(s) 9, 306, 639
PsrI GAACNNNNNNTAC 2 cut(s) 686, 718
PstNI CAGNNNCTG 3 cut(s) 41, 157, 1069
PsuI RGATCY 2 cut(s) 304, 552
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 5 cut(s) 149, 472, 704, 887, 914
RsaNI GTAC 5 cut(s) 148, 471, 703, 886, 913
RseI CAYNNNNRTG 1 cut(s) 989
SacI GAGCTC 1 cut(s) 123
SacII CCGCGG 1 cut(s) 518
SalI GTCGAC 1 cut(s) 508
SaqAI TTAA 1 cut(s) 879
SatI GCNGC 6 cut(s) 6, 357, 421, 838, 963, 1064
Sau3AI GATC 5 cut(s) 43, 304, 311, 552, 594
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 5 cut(s) 36, 96, 152, 369, 795
SduI GDGCHC 1 cut(s) 123
SfaNI GCATC 2 cut(s) 35, 276
SfoI GGCGCC 1 cut(s) 639
Sfr303I CCGCGG 1 cut(s) 518
SgrBI CCGCGG 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 989
SphI GCATGC 1 cut(s) 381
SspDI GGCGCC 1 cut(s) 637
SspMI CTAG 3 cut(s) 204, 282, 1107
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 5 cut(s) 34, 94, 150, 367, 793
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 2 cut(s) 611, 872
TaqI TCGA 3 cut(s) 509, 528, 593
TaqII GACCGA 2 cut(s) 157, 725
TauI GCSGC 4 cut(s) 8, 359, 423, 840
TfiI GAWTC 4 cut(s) 91, 440, 930, 1017
Tru1I TTAA 1 cut(s) 879
Tru9I TTAA 1 cut(s) 879
TscAI CASTG 3 cut(s) 53, 296, 951
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 2 cut(s) 962, 1063
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 1 cut(s) 168
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 3 cut(s) 53, 296, 951
Van91I CCANNNNNTGG 2 cut(s) 157, 486
XceI RCATGY 2 cut(s) 381, 1002
XmiI GTMKAC 3 cut(s) 509, 606, 756
XspI CTAG 3 cut(s) 204, 282, 1107
ZraI GACGTC 1 cut(s) 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.