Rh6BG452400

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0013470
Physical Location & Seq
Reverse (-)
66640376 .. 66642055
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG452400.1

Sequence Viewer

Length: 1164 bp
ATGTCCATGGGTTTCTCAACAATGGCAGCTCTGACTTCCTCTGCATCAACCCCAGAAACTGAGAATTCTGACGTAGAAAGGAGCACAAGCAGAAGCAACAAATCTGAGCCGTTGATTCCTGGACTTCCAGACGAGGTGGCAGAGCTGTGTCTTGTTTACCTTCCGTATCCGTACCAAGCTTTGGTGCGTTCGGTCTCTGCTTCGTGGAGGAGAGCCATTTCTGACCCGGCTTTTCTTCACTGCAAGAAGTCACTTTCGCTCCCGCTGCCTTATCTTTTCGTTTTCGCCTTCAACAAATCGACGGCCAGGATTCAGTGGCAAGCGCTCGACCCTCGATCGGGCCGTTGGTTTGTCCTACCTCCGATGCCGGGGCATTATTCCAAGCTCGCGAGTCCACCGGGGTTAGCGTGTGCCTCCTTGCCACGTCAGGGGATGCTTGTCGTGTTGGGTGGATCGGACGCGGAATCGTCTATGCGGTCCACTATTGTATACCGTACGTCTACCAATCAGTGGTCCAGCATGGCTCCCATGCCGACCGCCCGATCGTATTTTGACGCGGGAAGCATCAATAATAAGATCATAGCGGTCGGAGGTAGTGGGACCCGCGAGAATGACTCGATCAAGGCCGTTGAGTGTTATAACCCTGAAAATGACACATGGATGACACTGGCCACGATGCCAGCTGGACTTGCCAAGTATGACTCCCACGTGGTGGGGGATAAGATGTACGTCACCGAGGGGTGGACGTGGCCCTTCATGTTCTCGCCACGCGGCTTGGTCTACGACTCGAAGACAGACGCGTGGCGAGAGATGAGTCCTGGGATGAGGGAAGGGTGGACAGGTGTCAGCGTGGTCGTGGGCAACAAGTTATTAGTGATCTCGGAGTATGGAGATTGTCCGATGAAGGTTTATGACCATGATAAGGACACATGGCGGTACGTCGGCGGGGACAAGTTCCCGTGCGACTCACTTAGACGGCCTTTCGCTGTGAGCGCGGTGGAGGGGAGCATATATGTGGTGGCATGTGGGCTGAATGTGGGAATAGGAAGGCTGTCTGAATCAGAAGCAGAAAAGGGTGAGCTACAAGTTGAGTGGCAGGTTCTGCCAGCTCCAAGTGCTTTTAGGGGTTTTTCTCCTTCTAGTTGTGAAGTGCTTTATGCCTGA

Protein Analysis

387

Amino Acids

42.47

Weight (kDa)

5.92

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 134 - 274 4.9e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 137 - 298 1.3e-08 FBX42, beta-propeller domain
Kelch_1 PF01344 143 - 178 2.2e-06 Kelch motif
Beta-prop_Calicin PF13964 146 - 272 9.8e-12 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 148 - 316 7e-17 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 148 - 285 1.7e-08 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 180 - 227 4.9e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24540
fragaria_vesca FvH4_2g36671
malus_domestica MD08G1127900.v1.1 MD15G1107400.v1.1
prunus_persica Prupe.1G459700_v2.0.a1 Prupe.5G016700_v2.0.a1
pyrus_communis pycom08g10690 pycom15g09820
rosa_chinensis RchiOBHm_Chr6g0305031
rosa_laevigata RLG00000010926
rosa_multiflora Rmu_sc0009145.1_g000002
rosa_roxburghii Rroxscaffold_7G00163240
rosa_rugosa Rorug06G0336900
rosa_samantha Rh6AG448500 Rh6BG452400 Rh6CG462200 Rh6DG449100
rosa_wichuraiana Rw6G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 639
Acc36I ACCTGC 1 cut(s) 1087
AccB7I CCANNNNNTGG 3 cut(s) 181, 510, 712
AccI GTMKAC 3 cut(s) 489, 500, 780
AccII CGCG 7 cut(s) 389, 461, 557, 606, 771, 800, 995
AclWI GGATC 1 cut(s) 460
AcoI YGGCCR 2 cut(s) 303, 669
AcsI RAATTY 1 cut(s) 64
AcvI CACGTG 1 cut(s) 709
AdeI CACNNNGTG 1 cut(s) 712
AfaI GTAC 4 cut(s) 173, 496, 728, 938
AfeI AGCGCT 1 cut(s) 324
AfiI CCNNNNNNNGG 9 cut(s) 181, 337, 338, 368, 428, 510, 712, 741, 922
AflIII ACRYGT 1 cut(s) 798
AgsI TTSAA 1 cut(s) 292
AjiI CACGTC 2 cut(s) 425, 747
AjnI CCWGG 3 cut(s) 118, 305, 817
AluBI AGCT 7 cut(s) 29, 145, 179, 385, 683, 1081, 1109
AluI AGCT 7 cut(s) 29, 145, 179, 385, 683, 1081, 1109
Alw21I GWGCWC 1 cut(s) 86
Alw26I GTCTC 1 cut(s) 199
AlwI GGATC 1 cut(s) 460
AlwNI CAGNNNCTG 2 cut(s) 59, 1102
Aor51HI AGCGCT 1 cut(s) 324
AoxI GGCC 6 cut(s) 303, 340, 624, 669, 749, 977
ApeKI GCWGC 2 cut(s) 26, 265
ApoI RAATTY 1 cut(s) 64
AspLEI GCGC 2 cut(s) 325, 995
AspS9I GGNCC 5 cut(s) 340, 477, 513, 600, 750
AsuC2I CCSGG 3 cut(s) 227, 369, 399
AsuHPI GGTGA 2 cut(s) 724, 1088
AvaII GGWCC 3 cut(s) 477, 513, 600
BalI TGGCCA 1 cut(s) 671
BbrPI CACGTG 1 cut(s) 709
BbsI GAAGAC 1 cut(s) 797
Bbv12I GWGCWC 1 cut(s) 86
BbvI GCAGC 2 cut(s) 38, 252
BceAI ACGGC 5 cut(s) 94, 318, 327, 611, 992
BciT130I CCWGG 3 cut(s) 120, 307, 819
BciVI GTATCC 1 cut(s) 177
BcnI CCSGG 3 cut(s) 227, 369, 399
BcoDI GTCTC 1 cut(s) 199
BfaI CTAG 1 cut(s) 1140
BfoI RGCGCY 1 cut(s) 326
BfuAI ACCTGC 1 cut(s) 1087
BfuI GTATCC 1 cut(s) 177
BisI GCNGC 3 cut(s) 27, 266, 772
BlsI GCNGC 3 cut(s) 28, 267, 773
Bme1390I CCNGG 6 cut(s) 120, 227, 307, 369, 399, 819
Bme18I GGWCC 3 cut(s) 477, 513, 600
BmgBI CACGTC 2 cut(s) 425, 747
BmgT120I GGNCC 5 cut(s) 340, 477, 513, 600, 750
BmiI GGNNCC 3 cut(s) 525, 601, 602
BmrFI CCNGG 6 cut(s) 120, 227, 307, 369, 399, 819
BmsI GCATC 5 cut(s) 53, 354, 423, 573, 666
BoxI GACNNNNGTC 1 cut(s) 842
BpiI GAAGAC 1 cut(s) 797
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BpuMI CCSGG 3 cut(s) 227, 369, 399
BsaAI YACGTR 1 cut(s) 709
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 5 cut(s) 6, 368, 398, 735, 818
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 9 cut(s) 181, 337, 338, 368, 428, 510, 712, 741, 922
Bse1I ACTGG 1 cut(s) 672
BseBI CCWGG 3 cut(s) 120, 307, 819
BseDI CCNNGG 5 cut(s) 6, 368, 398, 735, 818
BseGI GGATG 3 cut(s) 438, 666, 828
BseLI CCNNNNNNNGG 9 cut(s) 181, 337, 338, 368, 428, 510, 712, 741, 922
BseMII CTCAG 2 cut(s) 51, 96
BseNI ACTGG 1 cut(s) 672
BseRI GAGGAG 1 cut(s) 223
BseXI GCAGC 2 cut(s) 38, 252
Bsh1236I CGCG 7 cut(s) 389, 461, 557, 606, 771, 800, 995
Bsh1285I CGRYCG 4 cut(s) 338, 537, 545, 588
BshFI GGCC 6 cut(s) 305, 342, 626, 671, 751, 979
BsiEI CGRYCG 4 cut(s) 338, 537, 545, 588
BsiHKAI GWGCWC 1 cut(s) 86
BsiSI CCGG 3 cut(s) 227, 368, 398
BsiWI CGTACG 1 cut(s) 494
BslFI GGGAC 2 cut(s) 613, 962
BslI CCNNNNNNNGG 9 cut(s) 181, 337, 338, 368, 428, 510, 712, 741, 922
BsmAI GTCTC 1 cut(s) 199
BsmFI GGGAC 2 cut(s) 613, 962
BsnI GGCC 6 cut(s) 305, 342, 626, 671, 751, 979
Bso31I GGTCTC 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 6 cut(s) 335, 452, 542, 576, 618, 876
Bsp19I CCATGG 1 cut(s) 6
Bsp68I TCGCGA 1 cut(s) 389
BspANI GGCC 6 cut(s) 305, 342, 626, 671, 751, 979
BspCNI CTCAG 2 cut(s) 52, 97
BspFNI CGCG 7 cut(s) 389, 461, 557, 606, 771, 800, 995
BspLI GGNNCC 3 cut(s) 525, 601, 602
BspMI ACCTGC 1 cut(s) 1087
BspPI GGATC 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 199
BsrI ACTGG 1 cut(s) 672
BssECI CCNNGG 5 cut(s) 6, 368, 398, 735, 818
BssMI GATC 6 cut(s) 335, 452, 542, 576, 618, 876
BssNAI GTATAC 1 cut(s) 490
BssT1I CCWWGG 1 cut(s) 6
Bst1107I GTATAC 1 cut(s) 490
Bst2UI CCWGG 3 cut(s) 120, 307, 819
Bst4CI ACNGT 1 cut(s) 494
BstAPI GCANNNNNTGC 1 cut(s) 1102
BstBAI YACGTR 1 cut(s) 709
BstC8I GCNNGC 4 cut(s) 321, 387, 681, 1107
BstDEI CTNAG 3 cut(s) 60, 105, 971
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 3 cut(s) 438, 666, 828
BstFNI CGCG 7 cut(s) 389, 461, 557, 606, 771, 800, 995
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 2 cut(s) 325, 995
BstKTI GATC 6 cut(s) 338, 455, 545, 579, 621, 879
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 6 cut(s) 335, 452, 542, 576, 618, 876
BstMCI CGRYCG 4 cut(s) 338, 537, 545, 588
BstMWI GCNNNNNNNGC 5 cut(s) 265, 689, 992, 1102, 1115
BstNI CCWGG 3 cut(s) 120, 307, 819
BstNSI RCATGY 1 cut(s) 1026
BstPAI GACNNNNGTC 1 cut(s) 842
BstSCI CCNGG 6 cut(s) 118, 225, 305, 367, 397, 817
BstUI CGCG 7 cut(s) 389, 461, 557, 606, 771, 800, 995
BstV1I GCAGC 2 cut(s) 38, 252
BstV2I GAAGAC 1 cut(s) 797
BstZ17I GTATAC 1 cut(s) 490
BsuI GTATCC 1 cut(s) 177
BsuRI GGCC 6 cut(s) 305, 342, 626, 671, 751, 979
BtgI CCRYGG 1 cut(s) 6
BtrI CACGTC 2 cut(s) 425, 747
BtsCI GGATG 3 cut(s) 438, 666, 828
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 4 cut(s) 238, 320, 515, 665
BtuMI TCGCGA 1 cut(s) 389
BveI ACCTGC 1 cut(s) 1087
Cac8I GCNNGC 4 cut(s) 321, 387, 681, 1107
CaiI CAGNNNCTG 2 cut(s) 59, 1102
CfoI GCGC 2 cut(s) 325, 995
Cfr13I GGNCC 5 cut(s) 340, 477, 513, 600, 750
CseI GACGC 3 cut(s) 467, 563, 806
Csp6I GTAC 4 cut(s) 172, 495, 727, 937
CspCI CAANNNNNGTGG 4 cut(s) 756, 791, 1073, 1108
CviAII CATG 8 cut(s) 7, 520, 529, 657, 757, 917, 930, 1023
CviQI GTAC 4 cut(s) 172, 495, 727, 937
DdeI CTNAG 3 cut(s) 60, 105, 971
DpnI GATC 6 cut(s) 337, 454, 544, 578, 620, 878
DpnII GATC 6 cut(s) 335, 452, 542, 576, 618, 876
DraIII CACNNNGTG 1 cut(s) 712
EaeI YGGCCR 2 cut(s) 303, 669
Eco130I CCWWGG 1 cut(s) 6
Eco31I GGTCTC 1 cut(s) 199
Eco47I GGWCC 3 cut(s) 477, 513, 600
Eco47III AGCGCT 1 cut(s) 324
Eco72I CACGTG 1 cut(s) 709
EcoO109I RGGNCCY 1 cut(s) 600
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 3 cut(s) 118, 305, 817
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 8 cut(s) 10, 523, 532, 660, 760, 920, 933, 1026
FaqI GGGAC 2 cut(s) 613, 962
FatI CATG 8 cut(s) 6, 519, 528, 656, 756, 916, 929, 1022
FauI CCCGC 4 cut(s) 270, 550, 611, 938
FblI GTMKAC 3 cut(s) 489, 500, 780
Fnu4HI GCNGC 3 cut(s) 27, 266, 772
FokI GGATG 3 cut(s) 445, 673, 835
Fsp4HI GCNGC 3 cut(s) 27, 266, 772
FspBI CTAG 1 cut(s) 1140
GlaI GCGC 2 cut(s) 324, 994
GluI GCNGC 3 cut(s) 27, 266, 772
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 6 cut(s) 305, 342, 626, 671, 751, 979
HapII CCGG 3 cut(s) 227, 368, 398
HgaI GACGC 3 cut(s) 467, 563, 806
HhaI GCGC 2 cut(s) 325, 995
Hin1II CATG 8 cut(s) 10, 523, 532, 660, 760, 920, 933, 1026
Hin6I GCGC 2 cut(s) 323, 993
HinP1I GCGC 2 cut(s) 323, 993
HindIII AAGCTT 1 cut(s) 177
HpaII CCGG 3 cut(s) 227, 368, 398
HphI GGTGA 2 cut(s) 724, 1088
Hpy166II GTNNAC 8 cut(s) 157, 395, 480, 490, 501, 744, 781, 837
Hpy188III TCNNGA 2 cut(s) 128, 388
Hpy8I GTNNAC 8 cut(s) 157, 395, 480, 490, 501, 744, 781, 837
Hpy99I CGWCG 2 cut(s) 304, 944
HpyAV CCTTC 7 cut(s) 170, 298, 763, 824, 898, 1041, 1146
HpyCH4III ACNGT 1 cut(s) 494
HpyCH4IV ACGT 7 cut(s) 72, 424, 497, 708, 729, 746, 939
HpyCH4V TGCA 2 cut(s) 44, 243
HpyF10VI GCNNNNNNNGC 5 cut(s) 265, 689, 992, 1102, 1115
HpyF3I CTNAG 3 cut(s) 60, 105, 971
HpySE526I ACGT 7 cut(s) 72, 424, 497, 708, 729, 746, 939
Hsp92II CATG 8 cut(s) 10, 523, 532, 660, 760, 920, 933, 1026
HspAI GCGC 2 cut(s) 323, 993
KflI GGGWCCC 1 cut(s) 600
Kzo9I GATC 6 cut(s) 335, 452, 542, 576, 618, 876
LmnI GCTCC 5 cut(s) 81, 264, 529, 1005, 1114
Lsp1109I GCAGC 2 cut(s) 38, 252
LweI GCATC 5 cut(s) 53, 354, 423, 573, 666
MaeI CTAG 1 cut(s) 1140
MaeII ACGT 7 cut(s) 72, 424, 497, 708, 729, 746, 939
MaeIII GTNAC 2 cut(s) 249, 730
MalI GATC 6 cut(s) 337, 454, 544, 578, 620, 878
MboI GATC 6 cut(s) 335, 452, 542, 576, 618, 876
MboII GAAGA 2 cut(s) 227, 802
MhlI GDGCHC 1 cut(s) 86
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 1 cut(s) 64
MluI ACGCGT 1 cut(s) 798
MluNI TGGCCA 1 cut(s) 671
MlyI GAGTC 6 cut(s) 400, 608, 695, 779, 823, 959
MmeI TCCRAC 1 cut(s) 568
Mox20I TGGCCA 1 cut(s) 671
MscI TGGCCA 1 cut(s) 671
MslI CAYNNNNRTG 2 cut(s) 659, 1013
Msp20I TGGCCA 1 cut(s) 671
MspA1I CMGCKG 2 cut(s) 265, 683
MspI CCGG 3 cut(s) 227, 368, 398
MspR9I CCNGG 6 cut(s) 120, 227, 307, 369, 399, 819
MvaI CCWGG 3 cut(s) 120, 307, 819
MvnI CGCG 7 cut(s) 389, 461, 557, 606, 771, 800, 995
MwoI GCNNNNNNNGC 5 cut(s) 265, 689, 992, 1102, 1115
NciI CCSGG 3 cut(s) 227, 369, 399
NcoI CCATGG 1 cut(s) 6
NdeII GATC 6 cut(s) 335, 452, 542, 576, 618, 876
NlaIII CATG 8 cut(s) 10, 523, 532, 660, 760, 920, 933, 1026
NlaIV GGNNCC 3 cut(s) 525, 601, 602
NmuCI GTSAC 2 cut(s) 249, 730
NruI TCGCGA 1 cut(s) 389
NspI RCATGY 1 cut(s) 1026
PcsI WCGNNNNNNNCGW 1 cut(s) 340
PfeI GAWTC 4 cut(s) 115, 310, 464, 1058
Pfl23II CGTACG 1 cut(s) 494
PflMI CCANNNNNTGG 3 cut(s) 181, 510, 712
PfoI TCCNGGA 1 cut(s) 118
PkrI GCNGC 3 cut(s) 28, 267, 773
Ple19I CGATCG 2 cut(s) 338, 545
PleI GAGTC 6 cut(s) 399, 608, 695, 779, 822, 959
PmaCI CACGTG 1 cut(s) 709
PmlI CACGTG 1 cut(s) 709
PpsI GAGTC 6 cut(s) 399, 608, 695, 779, 822, 959
Ppu21I YACGTR 1 cut(s) 709
PpuMI RGGWCCY 1 cut(s) 600
PshAI GACNNNNGTC 1 cut(s) 842
PsiI TTATAA 1 cut(s) 639
Psp5II RGGWCCY 1 cut(s) 600
Psp6I CCWGG 3 cut(s) 118, 305, 817
PspCI CACGTG 1 cut(s) 709
PspGI CCWGG 3 cut(s) 118, 305, 817
PspLI CGTACG 1 cut(s) 494
PspN4I GGNNCC 3 cut(s) 525, 601, 602
PspPI GGNCC 5 cut(s) 340, 477, 513, 600, 750
PspPPI RGGWCCY 1 cut(s) 600
PstNI CAGNNNCTG 2 cut(s) 59, 1102
PvuI CGATCG 2 cut(s) 338, 545
PvuII CAGCTG 1 cut(s) 683
RruI TCGCGA 1 cut(s) 389
RsaI GTAC 4 cut(s) 173, 496, 728, 938
RsaNI GTAC 4 cut(s) 172, 495, 727, 937
RseI CAYNNNNRTG 2 cut(s) 659, 1013
SatI GCNGC 3 cut(s) 27, 266, 772
Sau3AI GATC 6 cut(s) 335, 452, 542, 576, 618, 876
Sau96I GGNCC 5 cut(s) 340, 477, 513, 600, 750
SchI GAGTC 6 cut(s) 400, 608, 695, 779, 823, 959
ScrFI CCNGG 6 cut(s) 120, 227, 307, 369, 399, 819
SduI GDGCHC 1 cut(s) 86
SfaNI GCATC 5 cut(s) 53, 354, 423, 573, 666
SinI GGWCC 3 cut(s) 477, 513, 600
SmiMI CAYNNNNRTG 2 cut(s) 659, 1013
Sse9I AATT 1 cut(s) 64
SspMI CTAG 1 cut(s) 1140
StyD4I CCNGG 6 cut(s) 118, 225, 305, 367, 397, 817
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 494
TaiI ACGT 7 cut(s) 75, 427, 500, 711, 732, 749, 942
TaqI TCGA 5 cut(s) 299, 327, 334, 617, 788
TaqII GACCGA 1 cut(s) 181
TasI AATT 1 cut(s) 64
TauI GCSGC 1 cut(s) 774
TfiI GAWTC 4 cut(s) 115, 310, 464, 1058
TscAI CASTG 4 cut(s) 245, 320, 515, 672
TseFI GTSAC 2 cut(s) 249, 730
TseI GCWGC 2 cut(s) 26, 265
Tsp45I GTSAC 2 cut(s) 249, 730
TspDTI ATGAA 2 cut(s) 745, 917
TspGWI ACGGA 2 cut(s) 153, 159
TspRI CASTG 4 cut(s) 245, 320, 515, 672
Van91I CCANNNNNTGG 3 cut(s) 181, 510, 712
VpaK11BI GGWCC 3 cut(s) 477, 513, 600
XapI RAATTY 1 cut(s) 64
XceI RCATGY 1 cut(s) 1026
XmiI GTMKAC 3 cut(s) 489, 500, 780
XspI CTAG 1 cut(s) 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.