AT2G24970

Spindle and kinetochore-associated protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006708
Physical Location & Seq
Reverse (-)
10619531 .. 10621307
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G24970.1

Sequence Viewer

Length: 459 bp
ATGACTCATAATCATCATCATCAAGCTGTTGACAATCTGCTAAATGTTTTCTCAAGAGCTTCTCATGATCTTACTGTTGTTCATTCCAAGCTCGACAAGGAGTTTCAGCAGATGTATCCTGCTAATGCGAATCCGATGAAGCTGATTCAGCGAATCAAGAAGTTGCAGGAAGATGTGACTCTTCTTAAACACCAATGCCTTGACCTATTGTCTGCTAAACAGGATTTGATTGATAAGGCACAGACTACACTTGTTGGAAACTGTAACCTTATTCAGAAAATGAATGCATCATTAGGAGAATCAACGAATGGCGATACCGATGATGCATTGGCTGATTTTAATCAGATCATTGACGAGTGGACAATGCAAGTTAGATCCAGAACAGTGGGAGAAACAGAGGACGCAGATAAAGAAGACATCAATAAGATGCTTTTCTCAGCTATTTGTCATACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA2

Protein Analysis

152

Amino Acids

17.23

Weight (kDa)

5.51

Isoelectric Point (pI)

23.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA2 PF16740 7 - 111 7.7e-36 Spindle and kinetochore-associated protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 369
AhdI GACNNNNNGTC 1 cut(s) 208
AluBI AGCT 5 cut(s) 26, 59, 91, 142, 440
AluI AGCT 5 cut(s) 26, 59, 91, 142, 440
AlwI GGATC 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 420
BciVI GTATCC 1 cut(s) 126
BfuI GTATCC 1 cut(s) 126
BmeRI GACNNNNNGTC 1 cut(s) 208
BmsI GCATC 3 cut(s) 296, 313, 417
BpiI GAAGAC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 37
BsaBI GATNNNNATC 1 cut(s) 339
Bse8I GATNNNNATC 1 cut(s) 339
BseJI GATNNNNATC 1 cut(s) 339
BseMII CTCAG 1 cut(s) 450
BsmI GAATGC 1 cut(s) 289
Bsp143I GATC 3 cut(s) 67, 345, 374
BspCNI CTCAG 1 cut(s) 449
BspHI TCATGA 1 cut(s) 64
BspPI GGATC 1 cut(s) 369
BssMI GATC 3 cut(s) 67, 345, 374
Bst4CI ACNGT 3 cut(s) 76, 263, 385
Bst6I CTCTTC 1 cut(s) 186
BstDEI CTNAG 1 cut(s) 436
BstKTI GATC 3 cut(s) 70, 348, 377
BstMBI GATC 3 cut(s) 67, 345, 374
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstV2I GAAGAC 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 374
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 1 cut(s) 374
BsuI GTATCC 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 390
CciI TCATGA 1 cut(s) 64
CseI GACGC 1 cut(s) 410
CviAII CATG 1 cut(s) 65
CviJI RGCY 6 cut(s) 26, 59, 91, 142, 332, 440
CviKI_1 RGCY 6 cut(s) 26, 59, 91, 142, 332, 440
DdeI CTNAG 1 cut(s) 436
DpnI GATC 3 cut(s) 69, 347, 376
DpnII GATC 3 cut(s) 67, 345, 374
DriI GACNNNNNGTC 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 186
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 186
EcoT22I ATGCAT 2 cut(s) 289, 328
FaeI CATG 1 cut(s) 68
FaiI YATR 3 cut(s) 9, 66, 450
FatI CATG 1 cut(s) 64
HgaI GACGC 1 cut(s) 410
Hin1II CATG 1 cut(s) 68
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 6 cut(s) 4, 130, 145, 153, 178, 299
Hpy166II GTNNAC 2 cut(s) 31, 360
Hpy188I TCNGA 3 cut(s) 135, 276, 345
Hpy188III TCNNGA 4 cut(s) 54, 65, 157, 378
Hpy8I GTNNAC 2 cut(s) 31, 360
HpyCH4III ACNGT 3 cut(s) 76, 263, 385
HpyCH4V TGCA 4 cut(s) 166, 287, 326, 367
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 1 cut(s) 436
Hsp92II CATG 1 cut(s) 68
Kzo9I GATC 3 cut(s) 67, 345, 374
LpnPI CCDG 4 cut(s) 132, 152, 206, 391
LweI GCATC 3 cut(s) 296, 313, 417
MaeIII GTNAC 2 cut(s) 175, 263
MalI GATC 3 cut(s) 69, 347, 376
MboI GATC 3 cut(s) 67, 345, 374
MboII GAAGA 3 cut(s) 173, 182, 425
MfeI CAATTG 1 cut(s) 454
MflI RGATCY 1 cut(s) 374
MluCI AATT 1 cut(s) 454
MlyI GAGTC 1 cut(s) 172
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 1 cut(s) 391
Mph1103I ATGCAT 2 cut(s) 289, 328
MseI TTAA 2 cut(s) 186, 339
MunI CAATTG 1 cut(s) 454
Mva1269I GAATGC 1 cut(s) 289
MwoI GCNNNNNNNGC 1 cut(s) 148
NdeII GATC 3 cut(s) 67, 345, 374
NlaIII CATG 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 175
NsiI ATGCAT 2 cut(s) 289, 328
PagI TCATGA 1 cut(s) 64
PctI GAATGC 1 cut(s) 289
PfeI GAWTC 4 cut(s) 130, 145, 153, 299
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PsuI RGATCY 1 cut(s) 374
SaqAI TTAA 2 cut(s) 186, 339
Sau3AI GATC 3 cut(s) 67, 345, 374
SchI GAGTC 1 cut(s) 172
SetI ASST 7 cut(s) 28, 61, 93, 144, 207, 270, 442
SfaNI GCATC 3 cut(s) 296, 313, 417
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 1 cut(s) 454
TaaI ACNGT 3 cut(s) 76, 263, 385
TaqI TCGA 1 cut(s) 93
TasI AATT 1 cut(s) 454
TfiI GAWTC 4 cut(s) 130, 145, 153, 299
Tru1I TTAA 2 cut(s) 186, 339
Tru9I TTAA 2 cut(s) 186, 339
TscAI CASTG 1 cut(s) 390
TseFI GTSAC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 3 cut(s) 71, 152, 296
TspRI CASTG 1 cut(s) 390
Zsp2I ATGCAT 2 cut(s) 289, 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.