Rroxscaffold_7G00168930

Spindle and kinetochore-associated protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006708
Physical Location & Seq
Reverse (-)
9643105 .. 9644952
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00168930.1

Sequence Viewer

Length: 501 bp
ATGGGGCAAGAGCATCATGAAGCGACGGAGGGGTTGGTGAGGCTGCTGACGAAAGCGAACCACGATCTGACAGTGGTTCAGCACAGGCTCGACAAGGAGTTTCAGCAAATTTATCCCCCAAATGCAAACCCGATGAAGCTGGTTTCTAGAATTAAGAAAATTCAGCAAGACTTGTCCACTTTGGAAGACCAGTGCCGTCAACTTCTAACAGCCAAGCAGGATTTGATTGACAAGGCTAGGACCACTCTCGTCGGGAACAGAAATCTAGTGCAGCGGATGGAGGCATCCATGGGCATTTCCCCCAATACTGATTCTGATGATTCTGCTTTTGCCAACTTCAATCAGGGGCCTGAAACAGAATCTTATGACTGTATTGATCCCTTTTCGCAGATCATCGATGAGTGGAAGGTCCAAGCTAGATCAAAAGCAGGTGATGAAATAGATACAGATGTGCATGATATCAATACGTTACTATTTTCAACCATAGTTTCAAGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA2

Protein Analysis

166

Amino Acids

18.75

Weight (kDa)

4.93

Isoelectric Point (pI)

30.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA2 PF16740 6 - 114 1.4e-43 Spindle and kinetochore-associated protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 419
AciI CCGC 1 cut(s) 274
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 2 cut(s) 108, 159
AgsI TTSAA 3 cut(s) 340, 480, 492
AluBI AGCT 2 cut(s) 139, 416
AluI AGCT 2 cut(s) 139, 416
AlwI GGATC 1 cut(s) 371
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 2 cut(s) 43, 271
ApoI RAATTY 2 cut(s) 108, 159
AspS9I GGNCC 3 cut(s) 240, 347, 409
AsuHPI GGTGA 2 cut(s) 49, 443
AvaII GGWCC 2 cut(s) 240, 409
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BbsI GAAGAC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 30, 283
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 180
BfaI CTAG 4 cut(s) 147, 237, 266, 417
BfuAI ACCTGC 1 cut(s) 419
BisI GCNGC 2 cut(s) 44, 272
BlsI GCNGC 2 cut(s) 45, 273
Bme18I GGWCC 2 cut(s) 240, 409
BmgT120I GGNCC 3 cut(s) 240, 347, 409
BmiI GGNNCC 1 cut(s) 348
BmsI GCATC 2 cut(s) 22, 293
BpiI GAAGAC 1 cut(s) 192
Bsa29I ATCGAT 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 288
Bse1I ACTGG 1 cut(s) 190
BseCI ATCGAT 1 cut(s) 396
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 2 cut(s) 282, 284
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 2 cut(s) 30, 283
BsgI GTGCAG 1 cut(s) 290
BshFI GGCC 1 cut(s) 349
BshVI ATCGAT 1 cut(s) 396
BsnI GGCC 1 cut(s) 349
Bsp143I GATC 4 cut(s) 64, 376, 390, 419
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 1 cut(s) 274
BspANI GGCC 1 cut(s) 349
BspDI ATCGAT 1 cut(s) 396
BspHI TCATGA 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 419
BspPI GGATC 1 cut(s) 371
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 4 cut(s) 64, 376, 390, 419
BssT1I CCWWGG 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 73, 371
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 282, 284
BstKTI GATC 4 cut(s) 67, 379, 393, 422
BstMBI GATC 4 cut(s) 64, 376, 390, 419
BstV1I GCAGC 2 cut(s) 30, 283
BstV2I GAAGAC 1 cut(s) 192
Bsu15I ATCGAT 1 cut(s) 396
BsuRI GGCC 1 cut(s) 349
BsuTUI ATCGAT 1 cut(s) 396
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 2 cut(s) 282, 284
BtsIMutI CAGTG 2 cut(s) 78, 197
BveI ACCTGC 1 cut(s) 419
CciI TCATGA 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 240, 347, 409
ClaI ATCGAT 1 cut(s) 396
CviAII CATG 3 cut(s) 17, 289, 455
CviJI RGCY 7 cut(s) 43, 88, 139, 212, 236, 349, 416
CviKI_1 RGCY 7 cut(s) 43, 88, 139, 212, 236, 349, 416
DpnI GATC 4 cut(s) 66, 378, 392, 421
DpnII GATC 4 cut(s) 64, 376, 390, 419
Eco130I CCWWGG 1 cut(s) 288
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 2 cut(s) 240, 409
EcoO109I RGGNCCY 1 cut(s) 347
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 3 cut(s) 20, 292, 458
FaiI YATR 5 cut(s) 18, 290, 366, 456, 485
FatI CATG 3 cut(s) 16, 288, 454
Fnu4HI GCNGC 2 cut(s) 44, 272
FokI GGATG 2 cut(s) 271, 289
Fsp4HI GCNGC 2 cut(s) 44, 272
FspBI CTAG 4 cut(s) 147, 237, 266, 417
GluI GCNGC 2 cut(s) 44, 272
HaeIII GGCC 1 cut(s) 349
Hin1II CATG 3 cut(s) 20, 292, 458
HincII GTYRAC 1 cut(s) 200
HindII GTYRAC 1 cut(s) 200
HinfI GANTC 3 cut(s) 311, 320, 359
HphI GGTGA 2 cut(s) 49, 443
Hpy166II GTNNAC 2 cut(s) 177, 200
Hpy188I TCNGA 2 cut(s) 69, 316
Hpy188III TCNNGA 3 cut(s) 17, 147, 253
Hpy8I GTNNAC 2 cut(s) 177, 200
Hpy99I CGWCG 2 cut(s) 28, 254
HpyAV CCTTC 1 cut(s) 400
HpyCH4III ACNGT 2 cut(s) 73, 371
HpyCH4IV ACGT 1 cut(s) 467
HpyCH4V TGCA 3 cut(s) 125, 271, 454
HpySE526I ACGT 1 cut(s) 467
Hsp92II CATG 3 cut(s) 20, 292, 458
Kzo9I GATC 4 cut(s) 64, 376, 390, 419
LpnPI CCDG 7 cut(s) 70, 125, 203, 203, 329, 363, 414
Lsp1109I GCAGC 2 cut(s) 30, 283
LweI GCATC 2 cut(s) 22, 293
MaeI CTAG 4 cut(s) 147, 237, 266, 417
MaeII ACGT 1 cut(s) 467
MaeIII GTNAC 1 cut(s) 468
MalI GATC 4 cut(s) 66, 378, 392, 421
MboI GATC 4 cut(s) 64, 376, 390, 419
MboII GAAGA 1 cut(s) 197
MluCI AATT 3 cut(s) 108, 150, 159
MnlI CCTC 3 cut(s) 22, 33, 274
MseI TTAA 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 274
NcoI CCATGG 1 cut(s) 288
NdeII GATC 4 cut(s) 64, 376, 390, 419
NlaIII CATG 3 cut(s) 20, 292, 458
NlaIV GGNNCC 1 cut(s) 348
PagI TCATGA 1 cut(s) 16
PaqCI CACCTGC 1 cut(s) 419
PfeI GAWTC 3 cut(s) 311, 320, 359
PkrI GCNGC 2 cut(s) 45, 273
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 3 cut(s) 240, 347, 409
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 2 cut(s) 44, 272
Sau3AI GATC 4 cut(s) 64, 376, 390, 419
Sau96I GGNCC 3 cut(s) 240, 347, 409
SetI ASST 5 cut(s) 141, 411, 418, 433, 470
SfaNI GCATC 2 cut(s) 22, 293
SinI GGWCC 2 cut(s) 240, 409
Sse9I AATT 3 cut(s) 108, 150, 159
SsiI CCGC 1 cut(s) 274
SspMI CTAG 4 cut(s) 147, 237, 266, 417
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 2 cut(s) 73, 371
TaiI ACGT 1 cut(s) 470
TaqI TCGA 2 cut(s) 90, 396
TasI AATT 3 cut(s) 108, 150, 159
TfiI GAWTC 3 cut(s) 311, 320, 359
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 2 cut(s) 78, 197
TseI GCWGC 2 cut(s) 43, 271
TspDTI ATGAA 3 cut(s) 33, 149, 450
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 2 cut(s) 78, 197
VpaK11BI GGWCC 2 cut(s) 240, 409
XapI RAATTY 2 cut(s) 108, 159
XbaI TCTAGA 1 cut(s) 146
XspI CTAG 4 cut(s) 147, 237, 266, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.