MD08G1052100.v1.1

Spindle and kinetochore-associated protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006708
Physical Location & Seq
Forward (+)
4092571 .. 4094554
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1052100.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGGGCAGGTAAACGAGCATCACGAAGCGACTGACGGCTTGGTGAAGCTGCTGACGAAGGCGAACCACGATCTGACGGTGGTTCAGCACCGGCTCGAGAGGGAGTTCCAGCAAATCTACCCGGATAATGCGAACCCGATGAAGCTGGTTTCGAGGATAAAGAAGATTCAAGAAGAATTGTCGACTTTGGAAGATCAGTGCCGCGAACTTCTTTCCGCCAAACAGGATTTGATTGATAAGGCTAGGACGACTTTGGTTGGGAACAGAAACCAATTGCAGCGGATGGAGGCATCGATGGGAGTTTTCGCCGACGCTGATTCGGATGATTCTGCTTTTGCTAACTTCAACCAGGTCATTGATGAGTGGACGGTTCAAGTCAGATCAAAAACGGGGGATGAGAACCGGGATTCTGATTCGGAGGATATCAACCAGTTACTCTTTTCGGCTATAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA2

Protein Analysis

152

Amino Acids

17.19

Weight (kDa)

4.78

Isoelectric Point (pI)

19.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA2 PF16740 8 - 116 5.7e-42 Spindle and kinetochore-associated protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 182
AccII CGCG 1 cut(s) 204
AciI CCGC 3 cut(s) 202, 216, 280
AgsI TTSAA 3 cut(s) 170, 346, 374
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 2 cut(s) 49, 145
AluI AGCT 2 cut(s) 49, 145
Ama87I CYCGRG 1 cut(s) 95
ApeKI GCWGC 2 cut(s) 49, 277
AsuC2I CCSGG 2 cut(s) 122, 404
AsuHPI GGTGA 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 95
BbvI GCAGC 2 cut(s) 36, 289
BccI CCATC 2 cut(s) 277, 289
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 350
BcnI CCSGG 2 cut(s) 122, 404
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 1 cut(s) 447
BisI GCNGC 3 cut(s) 50, 202, 278
BlsI GCNGC 3 cut(s) 51, 203, 279
Bme1390I CCNGG 3 cut(s) 122, 350, 404
BmeT110I CYCGRG 1 cut(s) 95
BmrFI CCNGG 3 cut(s) 122, 350, 404
BmsI GCATC 2 cut(s) 28, 299
BpuMI CCSGG 2 cut(s) 122, 404
Bsa29I ATCGAT 1 cut(s) 293
Bse118I RCCGGY 1 cut(s) 90
Bse1I ACTGG 1 cut(s) 430
BseBI CCWGG 1 cut(s) 350
BseCI ATCGAT 1 cut(s) 293
BseGI GGATG 3 cut(s) 288, 328, 400
BseNI ACTGG 1 cut(s) 430
BseXI GCAGC 2 cut(s) 36, 289
Bsh1236I CGCG 1 cut(s) 204
BshVI ATCGAT 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 3 cut(s) 91, 122, 403
BsoBI CYCGRG 1 cut(s) 95
Bsp143I GATC 3 cut(s) 70, 193, 380
BspACI CCGC 3 cut(s) 202, 216, 280
BspDI ATCGAT 1 cut(s) 293
BspFNI CGCG 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 90
BsrI ACTGG 1 cut(s) 430
BssAI RCCGGY 1 cut(s) 90
BssMI GATC 3 cut(s) 70, 193, 380
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 2 cut(s) 79, 370
BstF5I GGATG 3 cut(s) 288, 328, 400
BstFNI CGCG 1 cut(s) 204
BstKTI GATC 3 cut(s) 73, 196, 383
BstMBI GATC 3 cut(s) 70, 193, 380
BstNI CCWGG 1 cut(s) 350
BstSCI CCNGG 3 cut(s) 120, 348, 402
BstSFI CTRYAG 1 cut(s) 447
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 2 cut(s) 36, 289
Bsu15I ATCGAT 1 cut(s) 293
BsuTUI ATCGAT 1 cut(s) 293
BtsCI GGATG 3 cut(s) 288, 328, 400
BtsIMutI CAGTG 1 cut(s) 203
Cfr10I RCCGGY 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 293
CseI GACGC 1 cut(s) 320
CsiI ACCWGGT 1 cut(s) 348
CviJI RGCY 6 cut(s) 39, 49, 94, 145, 242, 446
CviKI_1 RGCY 6 cut(s) 39, 49, 94, 145, 242, 446
DpnI GATC 3 cut(s) 72, 195, 382
DpnII GATC 3 cut(s) 70, 193, 380
EciI GGCGGA 1 cut(s) 205
Eco32I GATATC 1 cut(s) 424
Eco88I CYCGRG 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 348
EcoRV GATATC 1 cut(s) 424
FaiI YATR 1 cut(s) 449
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 3 cut(s) 50, 202, 278
FokI GGATG 3 cut(s) 295, 335, 407
Fsp4HI GCNGC 3 cut(s) 50, 202, 278
FspBI CTAG 1 cut(s) 243
GluI GCNGC 3 cut(s) 50, 202, 278
HapII CCGG 3 cut(s) 91, 122, 403
HgaI GACGC 1 cut(s) 320
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HinfI GANTC 5 cut(s) 166, 317, 326, 407, 413
HpaII CCGG 3 cut(s) 91, 122, 403
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 3 cut(s) 13, 183, 366
Hpy188I TCNGA 5 cut(s) 75, 322, 380, 412, 418
Hpy188III TCNNGA 3 cut(s) 23, 97, 170
Hpy8I GTNNAC 3 cut(s) 13, 183, 366
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 1 cut(s) 52
HpyCH4III ACNGT 2 cut(s) 79, 370
HpyCH4V TGCA 1 cut(s) 277
Kzo9I GATC 3 cut(s) 70, 193, 380
LpnPI CCDG 9 cut(s) 104, 122, 131, 135, 209, 335, 362, 416, 443
Lsp1109I GCAGC 2 cut(s) 36, 289
LweI GCATC 2 cut(s) 28, 299
MabI ACCWGGT 1 cut(s) 348
MaeI CTAG 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 432
MalI GATC 3 cut(s) 72, 195, 382
MboI GATC 3 cut(s) 70, 193, 380
MboII GAAGA 3 cut(s) 175, 185, 203
MfeI CAATTG 1 cut(s) 272
MluCI AATT 2 cut(s) 176, 272
MnlI CCTC 4 cut(s) 93, 147, 280, 412
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 3 cut(s) 91, 122, 403
MspR9I CCNGG 3 cut(s) 122, 350, 404
MunI CAATTG 1 cut(s) 272
MvaI CCWGG 1 cut(s) 350
MvnI CGCG 1 cut(s) 204
NciI CCSGG 2 cut(s) 122, 404
NdeII GATC 3 cut(s) 70, 193, 380
PaeR7I CTCGAG 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PfeI GAWTC 5 cut(s) 166, 317, 326, 407, 413
PkrI GCNGC 3 cut(s) 51, 203, 279
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
SalI GTCGAC 1 cut(s) 181
SatI GCNGC 3 cut(s) 50, 202, 278
Sau3AI GATC 3 cut(s) 70, 193, 380
ScrFI CCNGG 3 cut(s) 122, 350, 404
SetI ASST 4 cut(s) 12, 51, 147, 354
SexAI ACCWGGT 1 cut(s) 348
SfaNI GCATC 2 cut(s) 28, 299
SfcI CTRYAG 1 cut(s) 447
Sfr274I CTCGAG 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 2 cut(s) 176, 272
SsiI CCGC 3 cut(s) 202, 216, 280
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 3 cut(s) 120, 348, 402
TaaI ACNGT 2 cut(s) 79, 370
TaqI TCGA 4 cut(s) 96, 152, 182, 293
TasI AATT 2 cut(s) 176, 272
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 5 cut(s) 166, 317, 326, 407, 413
TscAI CASTG 1 cut(s) 203
TseI GCWGC 2 cut(s) 49, 277
TspDTI ATGAA 1 cut(s) 155
TspRI CASTG 1 cut(s) 203
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 182
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.