MD15G1043400.v1.1

Spindle and kinetochore-associated protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006708
Physical Location & Seq
Reverse (-)
3011107 .. 3012663
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1043400.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGGACACGTAAGCGAACACCACGAAGCGACGGACGGCTTGGTGAAGCTGCTGACGAAAGCGAACCACGATCTGACGGTGGTTCAGCACCGGCTCGAGAGGGAGTTCCAGCAAATCTACCCGGACAATGCGAACCCGATGAAGCTGGTTTCGAGGATAAAGAAGATTCAAGAAGAATTGTCGACATTGGAAGATCACTGCCGCCAACTTCTCTCTGCCAAACAGGATTTGATTGATAAGGCGAGGACGACTCTGGTTGGGAACAGAAATCAGTTGCAGCGGATGGAGACGTCGATGGGAGTTTTCGCCGACGCTGATTCGGATGATTCTGCTTTTGCTAACTTCAACCAGGTCATTGATGAGTGGACGGCTCAAGTCAGATCAAAAACAGGGGATGAGAATCGCGATTCGGATTCGGAGGATATCAATCAGTTGCTCTTTTCGGCTATAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA2

Protein Analysis

152

Amino Acids

17.18

Weight (kDa)

5.05

Isoelectric Point (pI)

23.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA2 PF16740 8 - 116 8.4e-41 Spindle and kinetochore-associated protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 293
AccI GTMKAC 1 cut(s) 182
AccII CGCG 1 cut(s) 405
AciI CCGC 2 cut(s) 202, 280
AcyI GRCGYC 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 7
AgsI TTSAA 2 cut(s) 170, 346
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 2 cut(s) 49, 145
AluI AGCT 2 cut(s) 49, 145
Alw26I GTCTC 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 95
ApeKI GCWGC 2 cut(s) 49, 277
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 95
BbvI GCAGC 2 cut(s) 36, 289
BccI CCATC 2 cut(s) 277, 289
BceAI ACGGC 2 cut(s) 52, 384
BciT130I CCWGG 1 cut(s) 350
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 447
BisI GCNGC 3 cut(s) 50, 202, 278
BlsI GCNGC 3 cut(s) 51, 203, 279
Bme1390I CCNGG 2 cut(s) 122, 350
BmeT110I CYCGRG 1 cut(s) 95
BmrFI CCNGG 2 cut(s) 122, 350
BplI GAGNNNNNCTC 2 cut(s) 235, 267
BpuEI CTTGAG 1 cut(s) 357
BpuMI CCSGG 1 cut(s) 122
BsaAI YACGTR 1 cut(s) 10
BsaBI GATNNNNATC 2 cut(s) 399, 426
BsaHI GRCGYC 1 cut(s) 290
Bse118I RCCGGY 1 cut(s) 90
Bse8I GATNNNNATC 2 cut(s) 399, 426
BseBI CCWGG 1 cut(s) 350
BseGI GGATG 3 cut(s) 288, 328, 400
BseJI GATNNNNATC 2 cut(s) 399, 426
BseXI GCAGC 2 cut(s) 36, 289
Bsh1236I CGCG 1 cut(s) 405
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 91, 122
BslFI GGGAC 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 281
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 95
Bsp143I GATC 3 cut(s) 70, 193, 380
Bsp68I TCGCGA 1 cut(s) 405
BspACI CCGC 2 cut(s) 202, 280
BspFNI CGCG 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 90
BssAI RCCGGY 1 cut(s) 90
BssMI GATC 3 cut(s) 70, 193, 380
BssNI GRCGYC 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 1 cut(s) 79
BstACI GRCGYC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 10
BstF5I GGATG 3 cut(s) 288, 328, 400
BstFNI CGCG 1 cut(s) 405
BstKTI GATC 3 cut(s) 73, 196, 383
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 3 cut(s) 70, 193, 380
BstNI CCWGG 1 cut(s) 350
BstSCI CCNGG 2 cut(s) 120, 348
BstSFI CTRYAG 1 cut(s) 447
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 2 cut(s) 36, 289
BtsCI GGATG 3 cut(s) 288, 328, 400
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 196
BtuMI TCGCGA 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 90
CseI GACGC 1 cut(s) 320
CsiI ACCWGGT 1 cut(s) 348
CviJI RGCY 6 cut(s) 39, 49, 94, 145, 371, 446
CviKI_1 RGCY 6 cut(s) 39, 49, 94, 145, 371, 446
DpnI GATC 3 cut(s) 72, 195, 382
DpnII GATC 3 cut(s) 70, 193, 380
Eco32I GATATC 1 cut(s) 424
Eco88I CYCGRG 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 348
EcoRV GATATC 1 cut(s) 424
Esp3I CGTCTC 1 cut(s) 281
FaiI YATR 1 cut(s) 449
FaqI GGGAC 1 cut(s) 18
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 3 cut(s) 50, 202, 278
FokI GGATG 3 cut(s) 295, 335, 407
Fsp4HI GCNGC 3 cut(s) 50, 202, 278
GluI GCNGC 3 cut(s) 50, 202, 278
HapII CCGG 2 cut(s) 91, 122
HgaI GACGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HinfI GANTC 7 cut(s) 166, 250, 317, 326, 400, 407, 413
HpaII CCGG 2 cut(s) 91, 122
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 2 cut(s) 183, 366
Hpy188I TCNGA 5 cut(s) 75, 322, 380, 412, 418
Hpy188III TCNNGA 3 cut(s) 97, 170, 404
Hpy8I GTNNAC 2 cut(s) 183, 366
Hpy99I CGWCG 3 cut(s) 34, 295, 314
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 2 cut(s) 9, 290
HpyCH4V TGCA 1 cut(s) 277
HpySE526I ACGT 2 cut(s) 9, 290
Hsp92I GRCGYC 1 cut(s) 290
Kzo9I GATC 3 cut(s) 70, 193, 380
LpnPI CCDG 9 cut(s) 104, 122, 131, 135, 209, 239, 335, 362, 375
Lsp1109I GCAGC 2 cut(s) 36, 289
MabI ACCWGGT 1 cut(s) 348
MaeII ACGT 2 cut(s) 9, 290
MalI GATC 3 cut(s) 72, 195, 382
MboI GATC 3 cut(s) 70, 193, 380
MboII GAAGA 3 cut(s) 175, 185, 203
MluCI AATT 1 cut(s) 176
MlyI GAGTC 1 cut(s) 244
MnlI CCTC 4 cut(s) 93, 147, 237, 412
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 2 cut(s) 91, 122
MspR9I CCNGG 2 cut(s) 122, 350
MvaI CCWGG 1 cut(s) 350
MvnI CGCG 1 cut(s) 405
NciI CCSGG 1 cut(s) 122
NdeII GATC 3 cut(s) 70, 193, 380
NruI TCGCGA 1 cut(s) 405
PaeR7I CTCGAG 1 cut(s) 95
PfeI GAWTC 6 cut(s) 166, 317, 326, 400, 407, 413
PkrI GCNGC 3 cut(s) 51, 203, 279
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
Ppu21I YACGTR 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
RruI TCGCGA 1 cut(s) 405
SalI GTCGAC 1 cut(s) 181
SatI GCNGC 3 cut(s) 50, 202, 278
Sau3AI GATC 3 cut(s) 70, 193, 380
SchI GAGTC 1 cut(s) 244
ScrFI CCNGG 2 cut(s) 122, 350
SetI ASST 5 cut(s) 12, 51, 147, 293, 354
SexAI ACCWGGT 1 cut(s) 348
SfcI CTRYAG 1 cut(s) 447
Sfr274I CTCGAG 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 95
SmlI CTYRAG 2 cut(s) 95, 372
SmoI CTYRAG 2 cut(s) 95, 372
Sse9I AATT 1 cut(s) 176
SsiI CCGC 2 cut(s) 202, 280
StyD4I CCNGG 2 cut(s) 120, 348
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 2 cut(s) 12, 293
TaqI TCGA 4 cut(s) 96, 152, 182, 293
TasI AATT 1 cut(s) 176
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 6 cut(s) 166, 317, 326, 400, 407, 413
TscAI CASTG 1 cut(s) 203
TseI GCWGC 2 cut(s) 49, 277
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 203
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 182
ZraI GACGTC 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.