pycom08g04350

Spindle and kinetochore-associated protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006708
Physical Location & Seq
Forward (+)
3353226 .. 3354548
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04350.5

Sequence Viewer

Length: 402 bp
ATGGGACACGTAAGCGAGCATCACGAAGCGACGGACGGCTTGGTGAAGCTGCTGACGAAGGCGAACCACGATCTGACGGTGGTTCAGCACCGGCTCGAGAGGGAGTTCCAGCAAATCTACCCAGATAATGCGAACCCAATGAAGCTGGTTTCGAGGATAAAGAAGATTCAAGAAGAATTGTCGACTTTGGAAGATCAGTGCCGCGAGCTTCTTTCGGCCAAACAGGATTTGATTGATAAGGCTAGGACGACTCTGGTTGGGAATAGAAACCAATTGCAGCGGATGGAGGCATCGATGGGAATTTTCGCCTACGCTGATTCGGATGATTCTGCCTTTGCTAACTTCAATCAGGTCATTGATGAGTGGACGGTTCAAGTCAGATCAAAAACAGAGATGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA2

Protein Analysis

134

Amino Acids

15.23

Weight (kDa)

5.5

Isoelectric Point (pI)

22.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 182
AccII CGCG 1 cut(s) 204
AciI CCGC 2 cut(s) 202, 280
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 1 cut(s) 300
AflIII ACRYGT 1 cut(s) 7
AgsI TTSAA 3 cut(s) 170, 346, 374
AluBI AGCT 3 cut(s) 49, 145, 208
AluI AGCT 3 cut(s) 49, 145, 208
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 1 cut(s) 216
ApeKI GCWGC 2 cut(s) 49, 277
ApoI RAATTY 1 cut(s) 300
AsuHPI GGTGA 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 95
BbvI GCAGC 2 cut(s) 36, 289
BccI CCATC 3 cut(s) 277, 289, 388
BceAI ACGGC 1 cut(s) 52
BfaI CTAG 1 cut(s) 243
BisI GCNGC 3 cut(s) 50, 202, 278
BlsI GCNGC 3 cut(s) 51, 203, 279
BmeT110I CYCGRG 1 cut(s) 95
BmsI GCATC 2 cut(s) 28, 299
Bsa29I ATCGAT 1 cut(s) 293
BsaAI YACGTR 1 cut(s) 10
Bse118I RCCGGY 1 cut(s) 90
BseCI ATCGAT 1 cut(s) 293
BseGI GGATG 2 cut(s) 288, 328
BseXI GCAGC 2 cut(s) 36, 289
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 1 cut(s) 218
BshVI ATCGAT 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 218
BsoBI CYCGRG 1 cut(s) 95
Bsp143I GATC 3 cut(s) 70, 193, 380
BspACI CCGC 2 cut(s) 202, 280
BspANI GGCC 1 cut(s) 218
BspDI ATCGAT 1 cut(s) 293
BspFNI CGCG 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 90
BssAI RCCGGY 1 cut(s) 90
BssMI GATC 3 cut(s) 70, 193, 380
Bst4CI ACNGT 2 cut(s) 79, 370
BstBAI YACGTR 1 cut(s) 10
BstC8I GCNNGC 2 cut(s) 17, 206
BstF5I GGATG 2 cut(s) 288, 328
BstFNI CGCG 1 cut(s) 204
BstKTI GATC 3 cut(s) 73, 196, 383
BstMBI GATC 3 cut(s) 70, 193, 380
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 2 cut(s) 36, 289
Bsu15I ATCGAT 1 cut(s) 293
BsuRI GGCC 1 cut(s) 218
BsuTUI ATCGAT 1 cut(s) 293
BtsCI GGATG 2 cut(s) 288, 328
BtsIMutI CAGTG 1 cut(s) 203
Cac8I GCNNGC 2 cut(s) 17, 206
Cfr10I RCCGGY 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 293
CviJI RGCY 7 cut(s) 39, 49, 94, 145, 208, 218, 242
CviKI_1 RGCY 7 cut(s) 39, 49, 94, 145, 208, 218, 242
DpnI GATC 3 cut(s) 72, 195, 382
DpnII GATC 3 cut(s) 70, 193, 380
EaeI YGGCCR 1 cut(s) 216
Eco88I CYCGRG 1 cut(s) 95
FaqI GGGAC 1 cut(s) 18
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 3 cut(s) 50, 202, 278
FokI GGATG 2 cut(s) 295, 335
Fsp4HI GCNGC 3 cut(s) 50, 202, 278
FspBI CTAG 1 cut(s) 243
GluI GCNGC 3 cut(s) 50, 202, 278
HaeIII GGCC 1 cut(s) 218
HapII CCGG 1 cut(s) 91
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HinfI GANTC 4 cut(s) 166, 250, 317, 326
HpaII CCGG 1 cut(s) 91
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 2 cut(s) 183, 366
Hpy188I TCNGA 3 cut(s) 75, 322, 380
Hpy188III TCNNGA 3 cut(s) 23, 97, 170
Hpy8I GTNNAC 2 cut(s) 183, 366
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 52
HpyCH4III ACNGT 2 cut(s) 79, 370
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 1 cut(s) 277
HpySE526I ACGT 1 cut(s) 9
Kzo9I GATC 3 cut(s) 70, 193, 380
LpnPI CCDG 7 cut(s) 104, 122, 131, 135, 209, 239, 335
Lsp1109I GCAGC 2 cut(s) 36, 289
LweI GCATC 2 cut(s) 28, 299
MaeI CTAG 1 cut(s) 243
MaeII ACGT 1 cut(s) 9
MalI GATC 3 cut(s) 72, 195, 382
MboI GATC 3 cut(s) 70, 193, 380
MboII GAAGA 3 cut(s) 175, 185, 203
MfeI CAATTG 1 cut(s) 272
MluCI AATT 3 cut(s) 176, 272, 300
MlyI GAGTC 1 cut(s) 244
MnlI CCTC 3 cut(s) 93, 147, 280
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 1 cut(s) 91
MunI CAATTG 1 cut(s) 272
MvnI CGCG 1 cut(s) 204
NdeII GATC 3 cut(s) 70, 193, 380
PaeR7I CTCGAG 1 cut(s) 95
PfeI GAWTC 3 cut(s) 166, 317, 326
PkrI GCNGC 3 cut(s) 51, 203, 279
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
Ppu21I YACGTR 1 cut(s) 10
SalI GTCGAC 1 cut(s) 181
SatI GCNGC 3 cut(s) 50, 202, 278
Sau3AI GATC 3 cut(s) 70, 193, 380
SchI GAGTC 1 cut(s) 244
SetI ASST 5 cut(s) 12, 51, 147, 210, 354
SfaNI GCATC 2 cut(s) 28, 299
Sfr274I CTCGAG 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 3 cut(s) 176, 272, 300
SsiI CCGC 2 cut(s) 202, 280
SspMI CTAG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 79, 370
TaiI ACGT 1 cut(s) 12
TaqI TCGA 4 cut(s) 96, 152, 182, 293
TasI AATT 3 cut(s) 176, 272, 300
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 3 cut(s) 166, 317, 326
TscAI CASTG 1 cut(s) 203
TseI GCWGC 2 cut(s) 49, 277
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 203
XapI RAATTY 1 cut(s) 300
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 182
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.