FvH4_2g41341

Spindle and kinetochore-associated protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006708
Physical Location & Seq
Reverse (-)
29255036 .. 29257340
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g41341.t1

Sequence Viewer

Length: 492 bp
ATGAGTCGAAAAGGAAAATCTTTATTCGACCGGAAAATGGGACAAGAGCATCATGAAGCGACGGAGGGGTTGGTGAGGCTGCTGACGAAAGCGAACCACGATCTGACAGCGGTTCAACACAGGCTCGACAAGGAGTTTCAACAAATCTATCCCCAGAATGCAAACCCGATGAAGCTGGTTTCCAGAATAAAGAAAATTCAGCAAGACTTGTCCACTTTGGAAGACCAGTGCCGTCACCTTCTAACAGCCAAGCAGGATTTGATTGACAAGGCTAGGACCACTCTCGTCGGTAACAGAAATCTAGTGCAGAGGATGGAGGCTTCCATGGGCATTTCCCCCACTACTGATTCTCATGATTCTGCTTTTGCCAACTTCAATCAGATCATCGATGAGTGGAAGGTCCAAGCTAGAACAAAAGCAGGTGATGAGATGGAAACAGATGTGGACGATATTAATACATTACTATTTTCAACTATAGTTTCAAGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA2

Protein Analysis

164

Amino Acids

18.56

Weight (kDa)

6.59

Isoelectric Point (pI)

25.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA2 PF16740 18 - 126 8.6e-42 Spindle and kinetochore-associated protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 410
Acc36I ACCTGC 1 cut(s) 410
AciI CCGC 1 cut(s) 110
AcsI RAATTY 1 cut(s) 195
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 5 cut(s) 116, 140, 376, 471, 483
AluBI AGCT 2 cut(s) 175, 407
AluI AGCT 2 cut(s) 175, 407
ApeKI GCWGC 1 cut(s) 79
ApoI RAATTY 1 cut(s) 195
AseI ATTAAT 1 cut(s) 453
AspS9I GGNCC 2 cut(s) 276, 400
AsuHPI GGTGA 3 cut(s) 85, 227, 434
AvaII GGWCC 2 cut(s) 276, 400
BbsI GAAGAC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 66
BccI CCATC 2 cut(s) 307, 424
BceAI ACGGC 1 cut(s) 216
BfaI CTAG 3 cut(s) 273, 302, 408
BfmI CTRYAG 1 cut(s) 474
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 1 cut(s) 80
BlsI GCNGC 1 cut(s) 81
Bme18I GGWCC 2 cut(s) 276, 400
BmgT120I GGNCC 2 cut(s) 276, 400
BmsI GCATC 1 cut(s) 58
BpiI GAAGAC 1 cut(s) 228
Bsa29I ATCGAT 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 226
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 37
BseNI ACTGG 1 cut(s) 226
BseXI GCAGC 1 cut(s) 66
BsgI GTGCAG 1 cut(s) 326
Bsh1285I CGRYCG 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 387
BsiEI CGRYCG 1 cut(s) 31
BsiSI CCGG 1 cut(s) 31
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 54
BsmI GAATGC 1 cut(s) 163
Bsp143I GATC 2 cut(s) 100, 381
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 1 cut(s) 110
BspDI ATCGAT 1 cut(s) 387
BspHI TCATGA 2 cut(s) 52, 352
BspMI ACCTGC 1 cut(s) 410
BsrI ACTGG 1 cut(s) 226
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 2 cut(s) 100, 381
BssT1I CCWWGG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 318
BstKTI GATC 2 cut(s) 103, 384
BstMBI GATC 2 cut(s) 100, 381
BstMCI CGRYCG 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 474
BstV1I GCAGC 1 cut(s) 66
BstV2I GAAGAC 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 387
BsuTUI ATCGAT 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 233
BveI ACCTGC 1 cut(s) 410
CciI TCATGA 2 cut(s) 52, 352
Cfr13I GGNCC 2 cut(s) 276, 400
ClaI ATCGAT 1 cut(s) 387
CviAII CATG 3 cut(s) 53, 325, 353
CviJI RGCY 7 cut(s) 79, 124, 175, 248, 272, 320, 407
CviKI_1 RGCY 7 cut(s) 79, 124, 175, 248, 272, 320, 407
DpnI GATC 2 cut(s) 102, 383
DpnII GATC 2 cut(s) 100, 381
Eco130I CCWWGG 1 cut(s) 324
Eco47I GGWCC 2 cut(s) 276, 400
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 3 cut(s) 56, 328, 356
FaiI YATR 4 cut(s) 54, 326, 354, 476
FaqI GGGAC 1 cut(s) 54
FatI CATG 3 cut(s) 52, 324, 352
Fnu4HI GCNGC 1 cut(s) 80
FokI GGATG 1 cut(s) 325
Fsp4HI GCNGC 1 cut(s) 80
FspBI CTAG 3 cut(s) 273, 302, 408
GluI GCNGC 1 cut(s) 80
HapII CCGG 1 cut(s) 31
Hin1II CATG 3 cut(s) 56, 328, 356
HinfI GANTC 3 cut(s) 4, 347, 356
HpaII CCGG 1 cut(s) 31
HphI GGTGA 3 cut(s) 85, 227, 434
Hpy166II GTNNAC 2 cut(s) 213, 445
Hpy188I TCNGA 2 cut(s) 105, 381
Hpy188III TCNNGA 3 cut(s) 53, 183, 353
Hpy8I GTNNAC 2 cut(s) 213, 445
Hpy99I CGWCG 2 cut(s) 64, 290
HpyAV CCTTC 2 cut(s) 248, 391
HpyCH4V TGCA 2 cut(s) 161, 307
Hsp92II CATG 3 cut(s) 56, 328, 356
Kzo9I GATC 2 cut(s) 100, 381
LpnPI CCDG 8 cut(s) 44, 106, 161, 167, 196, 239, 239, 405
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 1 cut(s) 58
MaeI CTAG 3 cut(s) 273, 302, 408
MaeIII GTNAC 2 cut(s) 233, 290
MalI GATC 2 cut(s) 102, 383
MboI GATC 2 cut(s) 100, 381
MboII GAAGA 1 cut(s) 233
MluCI AATT 1 cut(s) 195
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 4 cut(s) 58, 69, 303, 310
MseI TTAA 1 cut(s) 453
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 1 cut(s) 31
Mva1269I GAATGC 1 cut(s) 163
NcoI CCATGG 1 cut(s) 324
NdeII GATC 2 cut(s) 100, 381
NlaIII CATG 3 cut(s) 56, 328, 356
NmuCI GTSAC 1 cut(s) 233
PagI TCATGA 2 cut(s) 52, 352
PaqCI CACCTGC 1 cut(s) 410
PctI GAATGC 1 cut(s) 163
PfeI GAWTC 2 cut(s) 347, 356
PkrI GCNGC 1 cut(s) 81
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PshBI ATTAAT 1 cut(s) 453
PspPI GGNCC 2 cut(s) 276, 400
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 2 cut(s) 100, 381
Sau96I GGNCC 2 cut(s) 276, 400
SchI GAGTC 1 cut(s) 13
SetI ASST 5 cut(s) 177, 240, 402, 409, 424
SfaNI GCATC 1 cut(s) 58
SfcI CTRYAG 1 cut(s) 474
SinI GGWCC 2 cut(s) 276, 400
Sse9I AATT 1 cut(s) 195
SsiI CCGC 1 cut(s) 110
SspMI CTAG 3 cut(s) 273, 302, 408
StyI CCWWGG 1 cut(s) 324
TaqI TCGA 4 cut(s) 7, 27, 126, 387
TasI AATT 1 cut(s) 195
TfiI GAWTC 2 cut(s) 347, 356
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 1 cut(s) 233
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 1 cut(s) 79
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 2 cut(s) 69, 185
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 1 cut(s) 233
VpaK11BI GGWCC 2 cut(s) 276, 400
VspI ATTAAT 1 cut(s) 453
XapI RAATTY 1 cut(s) 195
XspI CTAG 3 cut(s) 273, 302, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.