AT2G25660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009462
Physical Location & Seq
Reverse (-)
10915307 .. 10927702
12396 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25660.4

Sequence Viewer

Length: 6501 bp
ATGAGTTTGAGATTGCAAAACCCTTTCCTTTCTACTCCTCTGCTTCATGGTTCTTTCAATCGCAGAGAGAAGAGAATCAATGTAGCTAGAAGGGCGTTTCGTAGCAAGCGTATATATTCGGAGAAGAAACAGAATGATTGGTTAGCTAAAGTTGCCAAATTTTCTCAATTTTGTGGGAAAAATGTGCAATTGCTTAGGAAGTCTCTTGATTCTAGAAGTAGAATGGAAGTTAAATGTTTGAAAGAGCCTTTCGTACGAAGCAAGGATTTGGTTAGGTCTTTGGCTCCTGTTTGGGAAGAGGGTTTGTTTTTCTTGAGGTGTTCTGTGTTCTTTGCTGTTATCTCTGGGGTTTGTTTATTGGTGTGGTATGGACAGAACAAGGCTCGTGTTTTTGTTGAGACTAAGCTTTTGCCATCTGTTTGCTCTGTGCTTAGTGAGACTATTCAGCGTGAGGTTGATTTTGGAAAGGTTAGAAGAGTTTCACCATTGTGCATTACACTTGAAGCAAGCTCCATTGGTCCTCATGGTGAAGAGTTTTCCTGTGGTGAGGTTCCGACTATGAAAGTTTGTGTCCGTCCTTTTGCTAGTCTTAGGAGGGGAAAGATTGTGGTTGATGCGATTCTAAGTAATCCAACAGTTTTGGTTGCACAAAAGAAAGATTTTACATGGTTAGGGATACCTCTATCTGACACTACTTTGCCAAGCCATTTGTCATCTGAAGAAGGGATTGATTTTCGCACCAAAACTAGGAGAGTTTCAAGGGAGGAAGCAGGGATCCGTTGGGATGAAGAAAGGGATAATGATGCAAGAAAAGCTGCAGAGATAGGCTATATCGTTCCTTGTAAGAATTACTCTCAAGCTAAAGATAATGCGGTGAAGCACGACAGGCGTTTTACGGAAATTGCAAATCCCAACTCCTTTATTTGCATGGATGAAAAGATGCATTCAGCTGAGCAACATTGCATGGACCCAGGTGTTGAGTATGATGTGAAGCATGCTGAATTGGAGAAATCATTTGGCATAAAAATTCCTGGTTCAGGGCTCAAGTTCTTGTCCAAAATGTTAAAAGTCCCGAGGAAGTACAAATTCAAATGGAATTCAAAATCACATAAGAATTCAATGTCTAATATTAGTGCAAAGAAGAGAATTCTTGAGCGCAGTGCTTCAGCAGCTCTATCTTATTTCCACAGTCTATCACAACAAAAGCTTGACGAGCCTTCAGTGTTATCAACAAATTATGATGGGTTGAGTTTGGATATGCTATTAGTTAAAGGTGATAGAGAAATCAGTAATCAATATGATCGACATGTGCCGTATGGTGAGCAATCATTAGCTAATGATTTGGACGGGAAAGGTTATCGAGTGAGGGGTAAAAGGCTCCTTGGTGTAAAAAAGGCTTCCACGTTAGACAAGTTCACAGTATCATGTGATCCATTCCTTATGACTGTTGATAGACTCTGTGCACTCTTACAAACTAAACGAAGCCCATCTGTTGAAGATATTGTGAATTCTTCTGAATCTGAGACTTTAAGCAGTCAGAGAGGGGATATATCTATGAATGTTGTTAATCAGAATACTGACGATGTTCCACATGGTAATCGAAGTGGAAATCAGCCTCGTGACTTTACGTTCAAAAAGCATGAGCATCAACCTGTAGCAAACCATTGGCGTCCCAGCTGGCCACGGAACAAAAAATTAAAAGAAGCAGTGTTTAATATTTTAACTGGTTCCTCTAAGAAGTTAACAGGAAGGGCAGATCCGAATGCTCCTCACTTGAGCGATGAACTGGAGAAGCTGCCTGCTGTTTATGTTGAGAAGACACTACCAGTTATGCTTGACTCTGTGCAGTTTAAAGGTGGGACACTTCTTCTATTAGCTTATGGCGATACAGAGCCCAGAGAAATGAGGAACGTCCATGGACATGTTAAGTTTCAGAATCATTATGGTCGTGTGTACGTGCAACTTGGTGGAAACTGTAATATGTGGAGATCAGATGTAACATCTGAAGATGGTGGGCTTTTGTCTGTTGATGTTTTTGTTGATACCGTCGAGCAGAACTGGCATGCAAATTTAAATGTTGCCAACTTCTTTGTCCCGATTTTTGAAAGAATTCTAGAGATTCCAATCGAGTGGTCTAAGGGAAGAGCTACTGGTGAGGTTCATCTATGTATGTCTAGAGGAGAAAGTTTTCCAAACCTACATGGCCAACTTGACGTAACAGGACTAGGCTTCCATATAAATGATGCACCTTCTTCGTTTTCTGATGTCTCAGCCAGCCTTTCTTTCCGCGGCCAGCGCATTTTCCTGCATAACGCTAATGGTTGGTTTGGAAAGGTTCCTTTGGAGGCTTCTGGAGATTTCGGTATACATCCAGATGAGGGGGAGTTTCATCTGATGTGTCAGGTTCCCTATGTGGAAATTAATGCTTTGATGAAAACTTTCAAGATGAAGCCCTTGTTTTTCCCGCTAGCTGGTTCTGTCACAGCTGTATTCAACTGTCAAGGCCCGCTTGATGCTCCCGTTTTTGTGGGAAGTTGCATGGTCTCTAGGAAGATAGCTTATCTGTCTCCAGATCTCCCTACATCTTTGGCATATGAGGCAATGCTGAAAAACAAGGAAGCTGGTGCAGTTGCAGCATTTGACCGTGTTCCATTTTCTTATCTTTCTGCTAATTTCACATTTAACACTGACAACTGTGTTGCTGATTTATATGGTATTAGAGCTACCCTTGTTGATGGTGGGGAGATTCGAGGAGCAGGGAATGCTTGGATCTGTCCAGAGGGGGAGGTGGATGATACCGCACTGGATGTGAACTTTTCAGGAAATATATCTTTTGATAAGGTTTTGCATCGTTATATGCCTGAATATTTTAACATCGGGATGCTGAAGTTGGGTGATTTAACTGGGGAAACAAAACTTTCTGGGGCCCTCTTGAAACCGAGGTTTGACATAAAATGGGCAGCCCCTAAAGCTGATGGCTCCTTGACTGATGCTCGGGGAGATATTGTGATATCACATGATAATATCATTGTTAATTCATCCTCTGTTGCTTTTGATCTATTCACAAAATTAGATACCTCATACCATGACCCTTGTTTGTCGCACCAAGACTTCACTCAAGGGGAGGCCATGCCATTTGTTGTTGAGGGGCTTGATTTGGATTTACGTATGCGTGGTTTTGAGTTTTTCAGCTTGGTCTCTTCCTATCCATTTGATTCACCACGACCTACACATTTAAAAGCAACAGGAAGGATCAAATTTCTGGGGAAGATAAAACGACACAGCACTACAAAAGATGGAGATGTTGGGTCAGACAAATGTGAAGATGCAGCTGCAATTTCTAGCCTCGATGGTGATATTTCCATTTCAAGTCTCAAGCTTAATCAGTTGATTTTGGCCCCTCAACTGTCTGGACGTTTGAGCGTTTCACGTGATCATGTTAAGCTTGATGCCGCGGGCCGACCAGATGAAAGTCTTACACTAGACTTTATAGGTCCACTACAGCCTAATTCTGACGAAAATGTACAAAGTGGAAAGTTGCTCTCCTTTTCTCTTCAAAAGGGACAACTAAGAGCTAACGCTTGTTTTCAGCCACAACAGTCCGCTACTTTGGAGATACGCAATTTTCCACTTGATGAGTTGGAGTTGGCCTCACTGAGAGGGTTGATTCAAAAGGCAGAGATTCAACTAAATCTTCAGAAGCGAAGAGGGCATGGCCTGTTGTCTGTAATTCGGCCAAAGTTTAGTGGAGTTCTAGGTGAAGCTTTAGATGTTGCAGTAAGATGGAGCGGTGACGTGTGTTTCATGTTGTCCGGAAGACTAGAGGTTATGATCACTGTTGAGAAAACTATTCTGGAACAAAGCAATAGCCGGTATGAGCTTCAAGGTGAATATGTATTGCCGGGTTCCCGTGACCGAGACCTTGGTCAGAAGGAAGCTGGCAGTTTTTTAATGAGAGCCATGACTGGTCATCTAGGAAGTGTTATATCCTCCATGGGAAGATGGAGAATGAGACTTGAAGTGCCTAAGGCAGAGGTTGCTGAGATGCTTCCTCTTGCAAGGCTTCTTTCAAGAAGTACAGATCCAGCTGTTCACTCAAGATCAAAGGATCTTTTCATCCAAAGCGTTCAAAATCTCTGTCTACAAGCTGAAAATCTTCGAGACTTGCTTGAGGAGATACGTGGGTATTATACTCCACCAAGTGAAGTCGTTCTTGAAGATCTAAGTCTTCCAGGTTTAGCTGAGCTCAAGGGTCATTGGCATGGTTCGCTTGATGCTAGTGGTGGAGGCAATGGAGACACTTTGGCCGAATTTGACTTCCATGGAGATGATTGGGAGTGGGGGACTTACAAAACACAGCGTGTTCTGGCTACTGGTTCGTATAACAATGATGATGGTTTACGTCTTAAGGAGATGCTCATTCAAAAAGGAAACGCTACACTGCACGCAGATGGGACCTTATTGGGGCCAAAAACAAATCTTCATTTTGCTGTTCTGAATTTCCCTGTTAGTTTGATACCTACCTTGGTTGAGGTTGTTGAATCATCAGCTACTGATATTGTTCACTCTTTGAGGAAACTCTTGTCACCAATCAAAGGCATTTTGCACATGGAAGGGGATCTTAGAGGGAGTCTTGAAAAACCAGAATGTGATGTACAAGTTAGGTTATTGGATGGTGCTGTTGGTGGTATAGACCTTGGACGAGCTGAAGTTTTTGCTTCTCTTACATCTAACAGTCGTTTTCTTTTCAACTCCAACTTTGAACCATTTGTCCAAAATGGTCATGTCCATATACAAGGAAGTGTTCCTGTTAGCTTTTCGCAGAAAAATATGTCTGAAGGAGAAGTCAGCGAAACAGATAGAGGTGGTGCAGTGAAAATCCCGAGTTGGGCAAAAGAGAAGGAAGATGATGAGAAGAGAACCTCAAGGGATAGGAGCGAAGAGAGATGGGACAGCCAACTAGCTGAGAGTCTCAAGGGTTTATACTGGAATATTTTAGATGCAGGAGAAGTTAGACTGGAGGCAGACATCAAGGATGGGGGAATGACTTTGTTAACAGCCATATCTCCTTATGCTAATTGGCTCCAAGGAAATGCTGATATCAGGCTTCAGGTCGGAGGTACAGTAGACCATCCAGTGCTTGATGGGTCTGCTTCATTCCACAGGGCGTCTATCTCTTCACCTGTGCTTCGTAAACCGCTCACAAACTTTGGTGGAACCTTACATGTTAAATCAAACAGGTTGTGCATCACCTCACTGGAAAGCAGGGTAAGTAGAAAGGGAAAGCTGGTTGTTAAAGGAAATCTTCCCCTTAGATCAAATGAAGCATCTGCTGGTGATGGAATAGAGCTCAAATGTGAAGTTCTAGAAGTACGAGCAAAGAATTTTTTAAGTTGTCAGGTTGATACTCAGCTGCAAATTACTGGATCAATGTTGCAGCCAACTATATCAGGAAATATTAAATTAAGCCAAGGAGAAGCTTATCTGCCTCATGATAAGGGTGGTGGAGCTGCCCCTTTAAATAGATTGGCAGCCAATCAGTATAGTATTCCAGGTGCTGCTATTAATCAAGCAGTTTCTTCACGATATTTTGCTCGATTTTTTGGCACTGAACGGGCTTCTTCAGGGATGAAATTCTCTCAGTCGACTGGTAAATCAAATTCTGTTGAAAAGGAGATTGAAGAAGTGAAAATGAAACCAAATATGGATATCCGTCTGAGTGATATGAAACTTGTTTTAGGACCAGAGCTGAGGATAATGTACCCTTTAATTCTTAATTTTGCTGTTAGTGGTGAGCTTGAACTTGATGGCATGGCTCATCCAAAATTTATCAAACCAAAGGGTGTCCTAACATTCGAAAACGGAGATGTCAATCTTGTGGCTACTCAGGTACGTCTGAAGAGGGAGCATCTCAACGTTGCAAAGTTTGAGCCTGAACATGGACTAGATCCTCTGCTAGATTTAGCCTTAGTTGGTTCAGAATGGCAATTTAGGGTTCAAAGTCGTGCCAGTAATTGGCAGGACAAACTTGTCGTGACTTCAACACGTTCTGTTGAACAGGATGCTCTCTCACCTTCGGAGGCTGCAAAGGTGTTTGAGAGCCAATTAGCAGAATCCATATTAGAAGGAGATGGACAACTTGCTTTCAAAAAACTTGCAACCGCAACACTTGGAACCATAATGCCAAGGATAGAAGGAAAAGGAGAGTTTGGTCAGGCGAGATGGAGGTTGGTGTATGCTCCGCAGATCCCAAGTTTACTCTCTGTTGATCCAACTGTGGATCCTCTCAAGTCTCTGGCGAGCAACATATCATTTGGAACGGAAGTTGAAGTGCAGCTCGGAAAACGTCTTCAGGCCTCTGTGGTAAGACAGATGAAAGATTCTGAGATGGCAATGCAATGGACACTTATTTACCAGCTCACGAGCCGCTTGCGTGTCCTTCTGCAATCTGCACCTTCCAAACGTTTGCTTTTTGAATACTCTGCTACATCACAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TamB

Protein Analysis

2166

Amino Acids

240.34

Weight (kDa)

7.43

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TamB PF04357 1753 - 2150 2e-16 TamB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 6029
AccB7I CCANNNNNTGG 1 cut(s) 6015
AccBSI CCGCTC 2 cut(s) 3789, 5200
AccI GTMKAC 4 cut(s) 2365, 4141, 5127, 5645
AccII CGCG 2 cut(s) 2289, 3455
AccIII TCCGGA 1 cut(s) 3812
AclI AACGTT 2 cut(s) 5916, 6463
AcoI YGGCCR 5 cut(s) 1679, 2203, 2290, 3734, 4305
AcvI CACGTG 1 cut(s) 3431
AcyI GRCGYC 2 cut(s) 1669, 5168
AdeI CACNNNGTG 2 cut(s) 4202, 4361
AfiI CCNNNNNNNGG 9 cut(s) 747, 1037, 2378, 2471, 4464, 4595, 4888, 5939, 6015
AflII CTTAAG 1 cut(s) 4406
AflIII ACRYGT 5 cut(s) 1306, 1921, 3795, 5224, 6044
AjiI CACGTC 1 cut(s) 3796
AjnI CCWGG 4 cut(s) 970, 1030, 4231, 5551
AleI CACNNNNGTG 1 cut(s) 487
AloI GAACNNNNNNTCC 2 cut(s) 4755, 4787
Alw21I GWGCWC 3 cut(s) 1465, 4248, 5352
Alw44I GTGCAC 1 cut(s) 1461
AlwNI CAGNNNCTG 2 cut(s) 4553, 5558
Ama87I CYCGRG 3 cut(s) 1072, 2994, 4882
Aor13HI TCCGGA 1 cut(s) 3812
ApaI GGGCCC 1 cut(s) 2929
ApaLI GTGCAC 1 cut(s) 1461
ArsI GACNNNNNNTTYG 4 cut(s) 391, 423, 4123, 4155
AseI ATTAAT 2 cut(s) 2421, 5565
Asp700I GAANNNNTTC 6 cut(s) 478, 2109, 2187, 2438, 4155, 4209
AspLEI GCGC 2 cut(s) 1158, 2298
AsuC2I CCSGG 1 cut(s) 3903
AsuII TTCGAA 1 cut(s) 5856
AsuNHI GCTAGC 1 cut(s) 2467
AvaI CYCGRG 3 cut(s) 1072, 2994, 4882
AvaII GGWCC 5 cut(s) 518, 967, 3494, 4455, 5741
AxyI CCTNAGG 1 cut(s) 4026
BaeGI GKGCMC 2 cut(s) 1465, 2929
BaeI ACNNNNGTAYC 2 cut(s) 4169, 4202
BalI TGGCCA 2 cut(s) 1681, 2205
BamHI GGATCC 2 cut(s) 774, 6280
BanII GRGCYC 5 cut(s) 1044, 1896, 2929, 4248, 5352
BauI CACGAG 3 cut(s) 384, 1617, 6421
BbrPI CACGTG 1 cut(s) 3431
BbsI GAAGAC 4 cut(s) 1823, 3823, 4220, 6341
Bbv12I GWGCWC 3 cut(s) 1465, 4248, 5352
BbvCI CCTCAGC 1 cut(s) 5750
BceAI ACGGC 1 cut(s) 1297
BcgI CGANNNNNNTGC 4 cut(s) 2235, 2269, 6412, 6446
BciT130I CCWGG 4 cut(s) 972, 1032, 4233, 5553
BciVI GTATCC 1 cut(s) 669
BclI TGATCA 2 cut(s) 3433, 3831
BcnI CCSGG 1 cut(s) 3903
BfmI CTRYAG 3 cut(s) 816, 1653, 3500
BfrI CTTAAG 1 cut(s) 4406
BfuI GTATCC 1 cut(s) 669
BglII AGATCT 2 cut(s) 2572, 4219
BlpI GCTNAGC 2 cut(s) 951, 4242
Bme1390I CCNGG 5 cut(s) 972, 1032, 3903, 4233, 5553
Bme18I GGWCC 5 cut(s) 518, 967, 3494, 4455, 5741
BmeT110I CYCGRG 3 cut(s) 1072, 2994, 4882
BmgBI CACGTC 1 cut(s) 3796
BmrFI CCNGG 5 cut(s) 972, 1032, 3903, 4233, 5553
BmrI ACTGGG 1 cut(s) 2913
BmtI GCTAGC 1 cut(s) 2471
BmuI ACTGGG 1 cut(s) 2913
BoxI GACNNNNGTC 1 cut(s) 3924
BpiI GAAGAC 4 cut(s) 1823, 3823, 4220, 6341
BplI GAGNNNNNCTC 2 cut(s) 3635, 3667
BpmI CTGGAG 4 cut(s) 1808, 2373, 2553, 5039
Bpu10I CCTNAGC 2 cut(s) 194, 5750
Bpu1102I GCTNAGC 2 cut(s) 951, 4242
Bpu14I TTCGAA 1 cut(s) 5856
BpuMI CCSGG 1 cut(s) 3903
BsaAI YACGTR 4 cut(s) 1957, 3167, 3431, 4181
BsaBI GATNNNNATC 2 cut(s) 2123, 5871
BsaHI GRCGYC 2 cut(s) 1669, 5168
BsaI GGTCTC 3 cut(s) 2548, 3202, 3912
BsaWI WCCGGW 1 cut(s) 3812
BsaXI ACNNNNNCTCC 4 cut(s) 2991, 3021, 5109, 5139
Bsc4I CCNNNNNNNGG 9 cut(s) 747, 1037, 2378, 2471, 4464, 4595, 4888, 5939, 6015
Bse118I RCCGGY 1 cut(s) 3870
Bse21I CCTNAGG 1 cut(s) 4026
Bse3DI GCAATG 5 cut(s) 958, 2607, 4297, 6399, 6404
Bse8I GATNNNNATC 2 cut(s) 2123, 5871
BseAI TCCGGA 1 cut(s) 3812
BseBI CCWGG 4 cut(s) 972, 1032, 4233, 5553
BseJI GATNNNNATC 2 cut(s) 2123, 5871
BseLI CCNNNNNNNGG 9 cut(s) 747, 1037, 2378, 2471, 4464, 4595, 4888, 5939, 6015
BseMI GCAATG 5 cut(s) 958, 2607, 4297, 6399, 6404
BseRI GAGGAG 5 cut(s) 27, 1758, 2193, 2766, 4187
BseSI GKGCMC 2 cut(s) 1465, 2929
BseYI CCCAGC 1 cut(s) 1673
BsgI GTGCAG 6 cut(s) 1865, 2646, 4427, 4890, 6353, 6435
Bsh1236I CGCG 2 cut(s) 2289, 3455
BsiHKAI GWGCWC 3 cut(s) 1465, 4248, 5352
BsiHKCI CYCGRG 3 cut(s) 1072, 2994, 4882
BsiSI CCGG 3 cut(s) 3813, 3871, 3902
BsiWI CGTACG 1 cut(s) 253
BslFI GGGAC 8 cut(s) 1055, 1656, 1873, 2078, 3576, 4357, 4468, 4964
BslI CCNNNNNNNGG 9 cut(s) 747, 1037, 2378, 2471, 4464, 4595, 4888, 5939, 6015
BsmFI GGGAC 8 cut(s) 1055, 1656, 1873, 2078, 3576, 4357, 4468, 4964
BsmI GAATGC 3 cut(s) 943, 1768, 2767
Bso31I GGTCTC 3 cut(s) 2548, 3202, 3912
BsoBI CYCGRG 3 cut(s) 1072, 2994, 4882
Bsp119I TTCGAA 1 cut(s) 5856
Bsp120I GGGCCC 1 cut(s) 2925
Bsp1286I GDGCHC 6 cut(s) 1044, 1465, 1896, 2929, 4248, 5352
Bsp13I TCCGGA 1 cut(s) 3812
Bsp1407I TGTACA 2 cut(s) 3523, 4654
Bsp1720I GCTNAGC 2 cut(s) 951, 4242
Bsp19I CCATGG 3 cut(s) 1915, 3993, 4321
BspEI TCCGGA 1 cut(s) 3812
BspFNI CGCG 2 cut(s) 2289, 3455
BspHI TCATGA 1 cut(s) 5491
BspMAI CTGCAG 1 cut(s) 820
BspOI GCTAGC 1 cut(s) 2471
BspQI GCTCTTC 1 cut(s) 2137
BspT104I TTCGAA 1 cut(s) 5856
BspTI CTTAAG 1 cut(s) 4406
BspTNI GGTCTC 3 cut(s) 2548, 3202, 3912
BsrBI CCGCTC 2 cut(s) 3789, 5200
BsrDI GCAATG 5 cut(s) 958, 2607, 4297, 6399, 6404
BsrFI RCCGGY 1 cut(s) 3870
BsrGI TGTACA 2 cut(s) 3523, 4654
BssAI RCCGGY 1 cut(s) 3870
BssNAI GTATAC 1 cut(s) 2366
BssNI GRCGYC 2 cut(s) 1669, 5168
BssSI CACGAG 3 cut(s) 384, 1617, 6421
Bst1107I GTATAC 1 cut(s) 2366
Bst2BI CACGAG 3 cut(s) 384, 1617, 6421
Bst2UI CCWGG 4 cut(s) 972, 1032, 4233, 5553
BstACI GRCGYC 2 cut(s) 1669, 5168
BstAFI CTTAAG 1 cut(s) 4406
BstAPI GCANNNNNTGC 2 cut(s) 2762, 4037
BstAUI TGTACA 2 cut(s) 3523, 4654
BstBAI YACGTR 4 cut(s) 1957, 3167, 3431, 4181
BstBI TTCGAA 1 cut(s) 5856
BstDSI CCRYGG 6 cut(s) 1682, 1915, 2287, 3453, 3993, 4321
BstENI CCTNNNNNAGG 1 cut(s) 1035
BstFNI CGCG 2 cut(s) 2289, 3455
BstHHI GCGC 2 cut(s) 1158, 2298
BstNI CCWGG 4 cut(s) 972, 1032, 4233, 5553
BstNSI RCATGY 5 cut(s) 998, 1310, 1925, 2066, 5228
BstPAI GACNNNNGTC 1 cut(s) 3924
BstSCI CCNGG 5 cut(s) 970, 1030, 3901, 4231, 5551
BstSFI CTRYAG 3 cut(s) 816, 1653, 3500
BstSLI GKGCMC 2 cut(s) 1465, 2929
BstSNI TACGTA 1 cut(s) 3167
BstUI CGCG 2 cut(s) 2289, 3455
BstV2I GAAGAC 4 cut(s) 1823, 3823, 4220, 6341
BstXI CCANNNNNNTGG 1 cut(s) 2130
BstZ17I GTATAC 1 cut(s) 2366
Bsu36I CCTNAGG 1 cut(s) 4026
BsuI GTATCC 1 cut(s) 669
BtgI CCRYGG 6 cut(s) 1682, 1915, 2287, 3453, 3993, 4321
BtgZI GCGATG 1 cut(s) 1794
BtrI CACGTC 1 cut(s) 3796
BtsI GCAGTG 4 cut(s) 1165, 1713, 4439, 4878
CaiI CAGNNNCTG 2 cut(s) 4553, 5558
CciI TCATGA 1 cut(s) 5491
CfoI GCGC 2 cut(s) 1158, 2298
Cfr10I RCCGGY 1 cut(s) 3870
Cfr42I CCGCGG 2 cut(s) 2290, 3456
CseI GACGC 2 cut(s) 1658, 5157
DraI TTTAAA 4 cut(s) 1852, 2073, 3237, 5520
DraIII CACNNNGTG 2 cut(s) 4202, 4361
DrdI GACNNNNNNGTC 1 cut(s) 6029
DseDI GACNNNNNNGTC 1 cut(s) 6029
EaeI YGGCCR 5 cut(s) 1679, 2203, 2290, 3734, 4305
Ecl136II GAGCTC 2 cut(s) 4246, 5350
Eco105I TACGTA 1 cut(s) 3167
Eco147I AGGCCT 1 cut(s) 6356
Eco24I GRGCYC 5 cut(s) 1044, 1896, 2929, 4248, 5352
Eco31I GGTCTC 3 cut(s) 2548, 3202, 3912
Eco32I GATATC 3 cut(s) 3012, 5101, 5710
Eco47I GGWCC 5 cut(s) 518, 967, 3494, 4455, 5741
Eco53kI GAGCTC 2 cut(s) 4246, 5350
Eco72I CACGTG 1 cut(s) 3431
Eco81I CCTNAGG 1 cut(s) 4026
Eco88I CYCGRG 3 cut(s) 1072, 2994, 4882
EcoICRI GAGCTC 2 cut(s) 4246, 5350
EcoNI CCTNNNNNAGG 1 cut(s) 1035
EcoO109I RGGNCCY 3 cut(s) 2925, 2926, 4455
EcoRI GAATTC 5 cut(s) 1096, 1114, 1146, 1507, 2109
EcoRII CCWGG 4 cut(s) 970, 1030, 4231, 5551
EcoRV GATATC 3 cut(s) 3012, 5101, 5710
EcoT22I ATGCAT 1 cut(s) 945
EcoT38I GRGCYC 5 cut(s) 1044, 1896, 2929, 4248, 5352
FalI AAGNNNNNCTT 8 cut(s) 2493, 2525, 4197, 4229, 4605, 4637, 5289, 5321
FaqI GGGAC 8 cut(s) 1055, 1656, 1873, 2078, 3576, 4357, 4468, 4964
FauI CCCGC 3 cut(s) 2472, 2514, 3448
FauNDI CATATG 1 cut(s) 2593
FbaI TGATCA 2 cut(s) 3433, 3831
FblI GTMKAC 4 cut(s) 2365, 4141, 5127, 5645
FriOI GRGCYC 5 cut(s) 1044, 1896, 2929, 4248, 5352
GlaI GCGC 2 cut(s) 1157, 2297
GsaI CCCAGC 1 cut(s) 1677
GsuI CTGGAG 4 cut(s) 1808, 2373, 2553, 5039
HapII CCGG 3 cut(s) 3813, 3871, 3902
HgaI GACGC 2 cut(s) 1658, 5157
HhaI GCGC 2 cut(s) 1158, 2298
Hin1I GRCGYC 2 cut(s) 1669, 5168
Hin6I GCGC 2 cut(s) 1156, 2296
HinP1I GCGC 2 cut(s) 1156, 2296
HincII GTYRAC 3 cut(s) 1743, 5055, 5646
HindII GTYRAC 3 cut(s) 1743, 5055, 5646
HindIII AAGCTT 6 cut(s) 404, 1205, 3377, 3443, 3762, 5478
HpaI GTTAAC 2 cut(s) 1743, 5055
HpaII CCGG 3 cut(s) 3813, 3871, 3902
Hpy99I CGWCG 1 cut(s) 2051
Hsp92I GRCGYC 2 cut(s) 1669, 5168
HspAI GCGC 2 cut(s) 1156, 2296
Kpn2I TCCGGA 1 cut(s) 3812
Ksp22I TGATCA 2 cut(s) 3433, 3831
KspAI GTTAAC 2 cut(s) 1743, 5055
KspI CCGCGG 2 cut(s) 2290, 3456
LguI GCTCTTC 1 cut(s) 2137
MaeIII GTNAC 8 cut(s) 1619, 1996, 2215, 2479, 3791, 3911, 4584, 6034
MbiI CCGCTC 2 cut(s) 3789, 5200
MfeI CAATTG 1 cut(s) 188
MhlI GDGCHC 6 cut(s) 1044, 1465, 1896, 2929, 4248, 5352
MlsI TGGCCA 2 cut(s) 1681, 2205
MluNI TGGCCA 2 cut(s) 1681, 2205
MlyI GAGTC 4 cut(s) 1449, 1832, 4639, 4978
MmeI TCCRAC 6 cut(s) 578, 656, 3621, 4779, 5095, 6296
Mox20I TGGCCA 2 cut(s) 1681, 2205
Mph1103I ATGCAT 1 cut(s) 945
MroI TCCGGA 1 cut(s) 3812
MroXI GAANNNNTTC 6 cut(s) 478, 2109, 2187, 2438, 4155, 4209
MscI TGGCCA 2 cut(s) 1681, 2205
MslI CAYNNNNRTG 8 cut(s) 487, 1920, 2238, 2373, 2879, 4260, 4326, 4449
Msp20I TGGCCA 2 cut(s) 1681, 2205
MspA1I CMGCKG 8 cut(s) 950, 1677, 2289, 2486, 3332, 3455, 4088, 5413
MspCI CTTAAG 1 cut(s) 4406
MspI CCGG 3 cut(s) 3813, 3871, 3902
MspR9I CCNGG 5 cut(s) 972, 1032, 3903, 4233, 5553
MunI CAATTG 1 cut(s) 188
Mva1269I GAATGC 3 cut(s) 943, 1768, 2767
MvaI CCWGG 4 cut(s) 972, 1032, 4233, 5553
MvnI CGCG 2 cut(s) 2289, 3455
NciI CCSGG 1 cut(s) 3903
NcoI CCATGG 3 cut(s) 1915, 3993, 4321
NdeI CATATG 1 cut(s) 2593
NheI GCTAGC 1 cut(s) 2467
NmuCI GTSAC 6 cut(s) 1619, 2479, 3791, 3911, 4584, 6034
NsiI ATGCAT 1 cut(s) 945
NspI RCATGY 5 cut(s) 998, 1310, 1925, 2066, 5228
NspV TTCGAA 1 cut(s) 5856
OliI CACNNNNGTG 1 cut(s) 487
PaeI GCATGC 2 cut(s) 998, 2066
PagI TCATGA 1 cut(s) 5491
PceI AGGCCT 1 cut(s) 6356
PciI ACATGT 3 cut(s) 1306, 1921, 5224
PciSI GCTCTTC 1 cut(s) 2137
PcsI WCGNNNNNNNCGW 1 cut(s) 106
PctI GAATGC 3 cut(s) 943, 1768, 2767
PdmI GAANNNNTTC 6 cut(s) 478, 2109, 2187, 2438, 4155, 4209
Pfl23II CGTACG 1 cut(s) 253
PflMI CCANNNNNTGG 1 cut(s) 6015
PleI GAGTC 4 cut(s) 1449, 1832, 4638, 4977
PmaCI CACGTG 1 cut(s) 3431
PmlI CACGTG 1 cut(s) 3431
PpsI GAGTC 4 cut(s) 1449, 1832, 4638, 4977
Ppu21I YACGTR 4 cut(s) 1957, 3167, 3431, 4181
PpuMI RGGWCCY 1 cut(s) 4455
PscI ACATGT 3 cut(s) 1306, 1921, 5224
PshAI GACNNNNGTC 1 cut(s) 3924
PshBI ATTAAT 2 cut(s) 2421, 5565
Psp124BI GAGCTC 2 cut(s) 4248, 5352
Psp1406I AACGTT 2 cut(s) 5916, 6463
Psp5II RGGWCCY 1 cut(s) 4455
Psp6I CCWGG 4 cut(s) 970, 1030, 4231, 5551
PspCI CACGTG 1 cut(s) 3431
PspFI CCCAGC 1 cut(s) 1673
PspGI CCWGG 4 cut(s) 970, 1030, 4231, 5551
PspLI CGTACG 1 cut(s) 253
PspOMI GGGCCC 1 cut(s) 2925
PspPPI RGGWCCY 1 cut(s) 4455
PstI CTGCAG 1 cut(s) 820
PstNI CAGNNNCTG 2 cut(s) 4553, 5558
PvuII CAGCTG 6 cut(s) 950, 1677, 2486, 3332, 4088, 5413
RseI CAYNNNNRTG 8 cut(s) 487, 1920, 2238, 2373, 2879, 4260, 4326, 4449
SacI GAGCTC 2 cut(s) 4248, 5352
SacII CCGCGG 2 cut(s) 2290, 3456
SalI GTCGAC 1 cut(s) 5644
SapI GCTCTTC 1 cut(s) 2137
SchI GAGTC 4 cut(s) 1449, 1832, 4639, 4978
ScrFI CCNGG 5 cut(s) 972, 1032, 3903, 4233, 5553
SduI GDGCHC 6 cut(s) 1044, 1465, 1896, 2929, 4248, 5352
SfcI CTRYAG 3 cut(s) 816, 1653, 3500
Sfr303I CCGCGG 2 cut(s) 2290, 3456
SfuI TTCGAA 1 cut(s) 5856
SgrBI CCGCGG 2 cut(s) 2290, 3456
SinI GGWCC 5 cut(s) 518, 967, 3494, 4455, 5741
SmiI ATTTAAAT 1 cut(s) 2073
SmiMI CAYNNNNRTG 8 cut(s) 487, 1920, 2238, 2373, 2879, 4260, 4326, 4449
SnaBI TACGTA 1 cut(s) 3167
SphI GCATGC 2 cut(s) 998, 2066
SseBI AGGCCT 1 cut(s) 6356
SspI AATATT 5 cut(s) 1129, 1717, 2867, 4993, 5458
SstI GAGCTC 2 cut(s) 4248, 5352
StuI AGGCCT 1 cut(s) 6356
StyD4I CCNGG 5 cut(s) 970, 1030, 3901, 4231, 5551
SwaI ATTTAAAT 1 cut(s) 2073
TaqII GACCGA 1 cut(s) 3930
TatI WGTACW 4 cut(s) 1080, 3523, 4076, 4654
TauI GCSGC 3 cut(s) 2292, 3455, 6429
TseFI GTSAC 6 cut(s) 1619, 2479, 3791, 3911, 4584, 6034
Tsp45I GTSAC 6 cut(s) 1619, 2479, 3791, 3911, 4584, 6034
TspGWI ACGGA 7 cut(s) 563, 767, 911, 1699, 5702, 5877, 6335
Van91I CCANNNNNTGG 1 cut(s) 6015
Vha464I CTTAAG 1 cut(s) 4406
VneI GTGCAC 1 cut(s) 1461
VpaK11BI GGWCC 5 cut(s) 518, 967, 3494, 4455, 5741
VspI ATTAAT 2 cut(s) 2421, 5565
XagI CCTNNNNNAGG 1 cut(s) 1035
XbaI TCTAGA 4 cut(s) 212, 2113, 2174, 5365
XceI RCATGY 5 cut(s) 998, 1310, 1925, 2066, 5228
XmiI GTMKAC 4 cut(s) 2365, 4141, 5127, 5645
XmnI GAANNNNTTC 6 cut(s) 478, 2109, 2187, 2438, 4155, 4209
Zsp2I ATGCAT 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.