FvH4_2g30180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009462
Physical Location & Seq
Reverse (-)
23335266 .. 23351276
16011 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30180.t1

Sequence Viewer

Length: 6507 bp
ATGAGCGGAAAACTCCATTGTGCATTTCTCGGGGCTCCGATTCACAGCTCTTTAAGTGGTAGGAACCGTGGGAGTTTAATTCATTGGGACAGCAGGCATGTGGGGAGGAGGGTTGTCCGCAGGTGTGTGAGCGAAAAGCAGAATTATTGGATTACTCAAGCAATTCGAGTTTCACATTTTTGGGGGAGAAATGTGGAGTTGTTAAAGAGAACCTTTGAGCTGAAAAATGGGAAGGTACAATGTGTTAAAGAGCCTTTTGCTCAAAGCAAGGCTCTAGTGCGGTCTTTATCTCCTTTATGGGAAGAGGGGTTACTTTTATTTAGGTGCTCTGTGTTTGTGGCCGTGATATCTGGGGTATGCTTGTTGGTATGGTACGGGCAGACAAAAGCCAAGGGTTTTATTGAAGCCAGGGTTTTGCCTTCTGTTTGCTCAGTGCTCAGTGAGTATATCCAGCGCGAAGTTGTTTTTGGTAAGGTTCGGAGGATATCACCTCTGAGCATCACATTGGAAGCCTGTTCAGTTGGCCCTCATGATGAAGAATTTTCTTGTGGTGAGGTCCCATCTATGAAACTTCGTGTTCGTCCTTTTGCAAGTCTGAGGAGGGGAAGGATTGTGATTGATGCAGTTTTATCCCATCCAACTGTCCTGATTGTGCAGAAGAAGGATTTTACATGGTTAGGGATACCCTCATCCGAAGGTTGTCTGCATGGGCACTTATCAACAGAAGAAGGAATTGATTATCGTACAAAAACCCGGAGGCTCGCTAGAGAGGAAGCTGGTGTTTGCTGGGAAAGAGAAAGGGATGAAGCGGCTAGGGAAGCTGCGGAGATGGGTTATATTATTTCAGAAAAGGGTTCCACTCCATCTGAAGGTGATGATTCAAAGGAAGTTGATAGCCATACAGGGGATTTAACGACCTCTGAATCGTTTTTATGCATGGATGAAAAGATGCACTGGAGAGATCACTGCATGGACACAGGCGTCGATTATGATATCAAGCATGCAGACTTGGAGAAATCATTGGGTGTAAAAATACCGGGTTCAGGGCTTAAATTTTGGTCCAGGGTGATAAAGGGGCCTAGAAAGCACAAATTCAAAAGGAATGGTTATGGGAATGATATCTCTGCATCTGGTATGAACGCAAAGAGAAGAATTCTTGGGGATAGTGCAGTGAGGGCACTTGCATATTTTCAGGGTTTGGCGCAGAGGAAGTCTGATGAACCTTCACAGTTGATGAATCTTGACACCTATTTGATGAAGAATGAGGTTGATACTAATGCCAACACTGCTGTCGTAGGTATAAGTCGAGAAACTGTCAGAGATGATAATCAGAATGGAAAAGGCTCTAGAGATTCAGCTGACCAAGCCCTTAAACAAAACCAGAATGCAATCAGTCATTTAAGCAGTTTTAATCTCAAAGATGATCCGTTGGATCAGTCCAATGTAGATGAGAAATCCAGTAACCTATCCACTGAAAAAGTTTCAGAAGCTAATACCAGTAGTAATGTAAAAGATAAGGGTTTAAGAAATGATGTTAACAATAGTCATTCTGAGGATGGTGAATCCGAAAGGCGAGCAGGTGAAACATTGCAGAATTCGATGTCCACAGTACCTTCTTTTACAACATATGATCATGGTCCCATCTGGCCCCCAAGTCCGAAATTAGGGTTTCCCTCATTTTCTATAAATGCTGGAGTTCCATTATCCCATCTTCTCTCTGGTCTCATTCAAAAGCTCACATCTAGCATGCGCCCCAGAGTTGAAGATATTGTTGCAGAACTTGTTGACGAGGTCAATATTGTGCAGCCTGAAGGCATTGAGAAGATGCTTCCAGTTACACTGGATTCTGTACAATTCAAAGGAGGAACACTCATGCTACTTGCATACGGTGACAGGGAACCTCGAGAGATGGAGAATGTCAATGGACACGTGAAGTTTCAAAATCACTACGGTCGCGTGCATGTACAAGTAAATGGCAACTGTAAGATGTGGAGGTCTGAAATCATGTCTGAAGATGGTGGCTGGTTGTCTACCGATGTATTTGTTGACATTGTTGAACAGAAATGGCACGCCAACCTAAAAGTTGCTAACCTCTTTGTTCCGCTATTCGAACGGATTTTAGCCATTCCAATAATTTGGTCTAAAGGAAGAGCCACTGGTGAGGTTCACTTGTGCATGTCAAGAGGAGAATCATTTCCCAATCTTCATGGACAGCTTGATGTGACAGGGTTGGCTTTTCAAACAATTGATGCTCCATCATCATTTTCCGACATCTCGGCAAGCTTATGTTTCCGTGGCCAGCGAATATTCCTACACAATGCAAGTGGCTGGTATGGGGATGTTCCTTTGGAAGCTTCTGGAGATTTTGGTATTCATCCTGATGAAGGAGAGTTTCATCTTATGTGTCAGGTTTCTTGTGTGGAAGTAAATGCCTTGATGAAAACTTTCAAGATGAAGCCTCTTATGTTTCCGCTGGCTGGTTCAGTGACTGCAGTGTTTAACTGTCAAGGTCCTTTGGATGCTCCTATATTTGTGGGAAGTGGAATGGTATCTAGAAGGATGTCCCAATCAGTTTCTGACTTTCCTGCATCTGCCGCATCAGAAGCAGTTTTGAAAAGTAAGGAAGCTGGTGCTGTGGCAGCATTTGATCGTGTACCATTTTCATGCGTATCAGCCAATTTCACTTTTAACACGGATAGTTGTGTTGCTGACTTGTATGGAATTAGAGCTAGTCTTGTTGATGGTGGTGAAATTCGTGGGGCTGGGAATGCTTGGATTTGCCCAGAGGGTGAGGTCGATGATACATCAATGGATGTGAATTTCTCTGGAAGTATGTGCTTTGATAAAATTCTGCATCGATATATTCCAGGGTATCTTCAACTGATGCCACTGAAATTAGGGGATTTAAATGGAGAAACAAAACTTTCTGGATCATTACTGAGACCGAGGTTTGACATTAAATGGACTGCTCCAAAAGCTGAAGGGTCCTTCAGTGATGCTCGAGGAGATATCATAATAGCGCATGATTCCATTACTGTTAGTTCCTCATCTACTGCCTTTGACTTGTCCTCAAAAGTTCAAACATCTTACAACGATAAAGATCGGAACAAAAGAGATGCTGAGACAAAGAGTGACATGCCATTTGTTGTTGAGGGAATCGACTTGGATTTACGTATGCGTGGTTTTGAGTTTTTCAGCTTGGTATCTTCTTATCCTTTTGATTCCCAAAAACCCATGCATTTGAAAGCAACAGGGAAGATCAAGTTTCAGGGAAAAGTATTAAAGCCTTTTAGTATTTCTACTGGAGAAGAATTTGATTCCGAGAGAAATAAACAACAGATGAATATGACTGATGAAGGAAAGACAGATAGTCTTGTAGGAGAGGTTTCAATTTCGGGTCTCAAATTGAACCAGTTGATGCTGGCCCCTCAGTTGGCTGGATCATTGAGCATTTCACGTGAGTGTATCAAGTTAGATGCCACAGGCAGGCCAGATGAAAGTCTTGTGGTGGAGTTTGTTGGGCCACTGAAACCTAACAGTGAAACTCACACGCAAAGTGGACAACTGCTATCTTTCTTCCTTCAAAAGGGCCAGCTAAAAGCGAACATTTGCTTCCAACCATTTCATTCTGCTAGCTTGGAGATACGTCAGTTACCACTTGATGAGTTGGAATTAGCTTCACTCCGTGGAACAATACAAAAGGCAGAAATTGAGCTTAATCTTCAGAAAAGAAGAGGTCATGGGCTGCTATCTGTACTTCGGCCAAAGTTTAGTGGTGTACTAGGTGAAGCCCTTGATGTTGCTGCTAGATGGAGTGGGGATGTTATCACTGTCGAGAAAACTGTCTTGGAGCAAAGCAATAGTCGTTATGAGCTTCAGGGTGAATACGTATTACCTGGCAGTCGAGATCGCAACCCTTCTGGGAAGGAAAGTGGAGGTCTATTGAAAAGAGCGATGGCTGGGAATCTGGGTAGTGTAATATCTTCTATGGGAAGGTGGAGAATGAGACTGGAGGTTCCTAGAGCTGAAGTTGCTGAGATGCTTCCCCTTGCCAGACTTGTTTCTCGAAGTACAGATCCTGCTGTTCACTCGAGATCAAAGGACTTCTTTGTTCAAAGCTTGCAGTCAGTTGGTTTATATACAGAAAGTCTCCAAGAACTGCTTGAGGTCATACGGGGACATTACACCCCATTAAGTGAAGTTATTCTGGAAGATGATCTCCCAGGTTTAACCGAGCTCAGAGGTAGCTGGCATGGCTCTCTTGATGCAAGCGGAGGTGGCAATGGGGACACAATGGCAGAATTTGATTTTCATGGGGAAGACTGGGAGTGGGGAACCTACAAAACTCAGCGTGTTCTGGCAGTAGGTGCATACAGCAATGATGATGGTTTGCGCCTAGAAAAGATCTTTATTCAGAAAGACAATGCCACAGTCCATGCAGATGGGACTTTGTTAGGGCCGAAAACAAATCTACACTTTGCTGTTTTGAACTTTCCTGTCAGTCTGGTTCCCACTGTGATTCAGGTCATTGAATCTTCTGCTACCGATGCTGTTCAATCTCTACGACAATTTTTAGCACCAATTAGAGGCATATTGCACATGGAAGGTGATCTTAGAGGGAGTCTGGCAAAACCGGAATGTGATGTGCAAGTAAGGCTCCTGGATGGTGCTGTTGGGGGGATTGATCTTGGACGAGCTGAAATTGTTGCTTCGCTCACCTCAACCAGTCGTTTTCTGTTCAATGCAAAATTTGAACCAATCATCCAAACTGGGCATGTACACATTCAGGGAAGTGTGCCTGTTTCTTTTGTTCAGAATAACTTGTTAGAGGAAGAAGATTCAGATAAGGATAAAAGCCGAGCTACTCCCTGGGACCATGGTTGGGTGAAGGAAAGAGGCAGAGTGTCTAGCGATGATGCCAGTGAAAAGAAACTTCCAAGAGAGAGAAATGAAGAAGGCTGGGATACAGGATTAGCTGAAAGCCTTAAAGGTTTAAACTGGAACATATTAGATGTAGGGGAAGTCAGAGTAGATGCTGATATAAAAGATGGGGGCATGATGATGCTGACTGCATTATCTCCTTATGCTAAATGGCTTCAGGGTAATGCTGACATCATGCTTCAGGTCAGAGGCACAGTTGAACAACCGGTGCTGGATGGATATGCATCCTTCCACAGGGCATCTATCTCATCCCCAGTTCTCTGGAAACCACTCACAAACTTCGGTGGCACAGTTCATGTAAAATCAAATAGGTTATGCATCACTTCATTGGAGAGTAGAGTAAGTAGAAGGGGTAAGCTGTTTGTCAAGGGAAACCTCCCCCTTAGAACAAGTGAAGCATCCCTTGGAGATAAAATTGAATTGAAATGTGAAGTTCTGGAAGTGCGGGCGAAAAATATTTTAAGTGCCCAGGTTGACACACAGATGCAGATAACTGGTTCCATATTGCAACCAAACATATCTGGAAATATTAAATTGAGCCATGGTGAAGCATATTTGCCACACGATAAAGGTAGTGGGGCTGCCCCCAATAGATTGGCAACAAGTGAGCCGAAGCTTCCTAGTATAGGTGTTGATCGAGCAGTTGCCTCAAGATATGTTTCACGGTTTTTCAGTTCACAGCCTGCTACTTCAAGGACAACATTCCCTCAACCTTCAGCGGTTAAGCAAGCTGAACAGGGGATAGAGCAAGTGAGCATCAAACCGAATGTAGATATTCAACTCAGTGATTTGAAGCTTGTTCTAGGACCAGAGCTGAGGATTGTTTATCCTTTAATTCTAAACTTTGCTGTTAGTGGAGAGCTAGAGTTAAATGGTCCAGCTCATCCCAAATCGATACAACCTAGAGGCATCTTAACTTTTGAAAATGGTGATGTCAATCTTGTCGCTACTCAGGTGAGGCTCAGGCAAGAGCATCTGAATATTGCAAAGTTTGAGCCTGAACATGGATTAGATCCAATGCTAGATCTAGTTTTAGTGGGGTCTGAGTGGCAATTCAGGATTCAAAGCCGAGCCAGCAACTGGCAGGAAAAACTGGTTGTGACCTCCACCCGTTCTGTGGAACAAGATGCCCTCTCACCTACTGAGGCTGCTAGAGTCTTTGAAAGTCAATTGGCCGAGTCCATCTTGGAAGGGGATGGGCAACTTGCATTCCAAAAGCTTGCAACTACAACACTAGAAAAACTAATGCCGAGGATAGAAGGGAAGGGAGAGTTTGGTCAGGCAAGGTGGAGGCTTGTGTATGCACCGCAGATCCCTAGCTTGCTTTCTGTTGATCCTACTGTGGATCCTCTCAAATCCCTGGCCAGTAATATTTCATTTGGTACGGAAGTTGAAGTCCAACTCGGGAAACGTCTTCAGGCCTCAATTGTTCGACAAATGAAGGATTCAGTAATGGAAATGCAATGGACACTGATCTACCAGCTTTCCAGCCGCTTGCGTGTTCTCCTGCAGTCTGCTCCGTCAAAACGCCTCATTTTTGAATACTCTGCTACATCTCAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TamB

Protein Analysis

2169

Amino Acids

239.38

Weight (kDa)

6.17

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TamB PF04357 1757 - 2017 9.7e-16 TamB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 111, 1568
AasI GACNNNNNNGTC 1 cut(s) 980
Acc36I ACCTGC 2 cut(s) 111, 1568
AccB7I CCANNNNNTGG 1 cut(s) 6021
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 2030
AccII CGCG 2 cut(s) 456, 1956
AcoI YGGCCR 5 cut(s) 339, 2296, 3761, 6114, 6303
AcvI CACGTG 2 cut(s) 1930, 3458
AcyI GRCGYC 1 cut(s) 981
AdeI CACNNNGTG 1 cut(s) 3686
AflIII ACRYGT 1 cut(s) 1927
AgeI ACCGGT 1 cut(s) 5146
AhdI GACNNNNNGTC 1 cut(s) 3369
AjnI CCWGG 9 cut(s) 407, 1061, 2866, 3895, 4222, 4659, 4868, 5409, 6300
AjuI GAANNNNNNNTTGG 6 cut(s) 450, 482, 3830, 3862, 5328, 5360
AleI CACNNNNGTG 1 cut(s) 3460
AloI GAACNNNNNNTCC 2 cut(s) 4746, 4778
Alw21I GWGCWC 3 cut(s) 329, 438, 4239
Alw26I GTCTC 6 cut(s) 1727, 2935, 3116, 3404, 4000, 4154
AlwNI CAGNNNCTG 4 cut(s) 2489, 2576, 4079, 6021
Ama87I CYCGRG 5 cut(s) 29, 1902, 3000, 4090, 6344
ApeKI GCWGC 7 cut(s) 821, 1804, 2639, 3745, 3803, 5522, 6089
ArsI GACNNNNNNTTYG 4 cut(s) 3125, 3157, 3847, 3879
AsiGI ACCGGT 1 cut(s) 5146
Asp700I GAANNNNTTC 3 cut(s) 2193, 2444, 4203
AspLEI GCGC 5 cut(s) 456, 1204, 1752, 3022, 4395
AsuC2I CCSGG 2 cut(s) 754, 1038
AsuII TTCGAA 1 cut(s) 2109
AsuNHI GCTAGC 1 cut(s) 3632
AvaI CYCGRG 5 cut(s) 29, 1902, 3000, 4090, 6344
AvaII GGWCC 8 cut(s) 556, 1059, 1637, 2510, 2985, 4873, 5747, 5816
BaeGI GKGCMC 3 cut(s) 714, 1180, 5410
BaeI ACNNNNGTAYC 4 cut(s) 3635, 3668, 6315, 6348
BalI TGGCCA 2 cut(s) 2298, 6305
BamHI GGATCC 1 cut(s) 6286
BanII GRGCYC 2 cut(s) 37, 4239
BarI GAAGNNNNNNTAC 2 cut(s) 294, 326
BbrPI CACGTG 2 cut(s) 1930, 3458
BbsI GAAGAC 2 cut(s) 4326, 6347
Bbv12I GWGCWC 3 cut(s) 329, 438, 4239
BbvCI CCTCAGC 1 cut(s) 5756
BbvI GCAGC 7 cut(s) 808, 1816, 2651, 3732, 3790, 5509, 6076
BceAI ACGGC 1 cut(s) 326
BciT130I CCWGG 9 cut(s) 409, 1063, 2868, 3897, 4224, 4661, 4870, 5411, 6302
BciVI GTATCC 2 cut(s) 675, 4957
BclI TGATCA 1 cut(s) 1630
BcnI CCSGG 2 cut(s) 754, 1038
BcoDI GTCTC 6 cut(s) 1727, 2935, 3116, 3404, 4000, 4154
BfmI CTRYAG 2 cut(s) 2490, 6449
BfuAI ACCTGC 2 cut(s) 111, 1568
BfuI GTATCC 2 cut(s) 675, 4957
BglII AGATCT 2 cut(s) 4404, 5965
Bme18I GGWCC 8 cut(s) 556, 1059, 1637, 2510, 2985, 4873, 5747, 5816
BmeRI GACNNNNNGTC 1 cut(s) 3369
BmeT110I CYCGRG 5 cut(s) 29, 1902, 3000, 4090, 6344
BmrI ACTGGG 3 cut(s) 4333, 4779, 5189
BmtI GCTAGC 1 cut(s) 3636
BmuI ACTGGG 3 cut(s) 4333, 4779, 5189
BpiI GAAGAC 2 cut(s) 4326, 6347
BplI GAGNNNNNCTC 2 cut(s) 1854, 1886
BpmI CTGGAG 5 cut(s) 976, 1713, 2379, 3324, 4031
Bpu10I CCTNAGC 2 cut(s) 5756, 5903
Bpu14I TTCGAA 1 cut(s) 2109
BpuEI CTTGAG 3 cut(s) 141, 4184, 5575
BpuMI CCSGG 2 cut(s) 754, 1038
Bsa29I ATCGAT 2 cut(s) 2857, 5834
BsaAI YACGTR 4 cut(s) 1930, 3173, 3458, 3889
BsaBI GATNNNNATC 4 cut(s) 1326, 3099, 5164, 5877
BsaHI GRCGYC 1 cut(s) 981
BsaI GGTCTC 3 cut(s) 1727, 2935, 3404
BsaWI WCCGGW 2 cut(s) 4633, 5146
Bse118I RCCGGY 1 cut(s) 5146
Bse3DI GCAATG 4 cut(s) 1586, 4288, 4384, 6410
Bse8I GATNNNNATC 4 cut(s) 1326, 3099, 5164, 5877
BseBI CCWGG 9 cut(s) 409, 1063, 2868, 3897, 4224, 4661, 4870, 5411, 6302
BseCI ATCGAT 2 cut(s) 2857, 5834
BseJI GATNNNNATC 4 cut(s) 1326, 3099, 5164, 5877
BseMI GCAATG 4 cut(s) 1586, 4288, 4384, 6410
BseRI GAGGAG 4 cut(s) 121, 613, 2199, 3018
BseSI GKGCMC 3 cut(s) 714, 1180, 5410
BseXI GCAGC 7 cut(s) 808, 1816, 2651, 3732, 3790, 5509, 6076
BseYI CCCAGC 4 cut(s) 786, 2762, 3959, 4959
BsgI GTGCAG 3 cut(s) 674, 1188, 1823
Bsh1236I CGCG 2 cut(s) 456, 1956
Bsh1285I CGRYCG 1 cut(s) 1954
BshTI ACCGGT 1 cut(s) 5146
BshVI ATCGAT 2 cut(s) 2857, 5834
BsiEI CGRYCG 1 cut(s) 1954
BsiHKAI GWGCWC 3 cut(s) 329, 438, 4239
BsiHKCI CYCGRG 5 cut(s) 29, 1902, 3000, 4090, 6344
BsiSI CCGG 4 cut(s) 754, 1037, 4634, 5147
BslFI GGGAC 8 cut(s) 101, 542, 1623, 2548, 4191, 4301, 4459, 4886
BsmAI GTCTC 6 cut(s) 1727, 2935, 3116, 3404, 4000, 4154
BsmFI GGGAC 8 cut(s) 101, 542, 1623, 2548, 4191, 4301, 4459, 4886
BsmI GAATGC 3 cut(s) 1390, 2773, 6149
Bso31I GGTCTC 3 cut(s) 1727, 2935, 3404
BsoBI CYCGRG 5 cut(s) 29, 1902, 3000, 4090, 6344
Bsp119I TTCGAA 1 cut(s) 2109
Bsp1286I GDGCHC 7 cut(s) 37, 329, 438, 714, 1180, 4239, 5410
Bsp1407I TGTACA 3 cut(s) 1849, 1963, 4777
Bsp19I CCATGG 2 cut(s) 4876, 5482
BspDI ATCGAT 2 cut(s) 2857, 5834
BspFNI CGCG 2 cut(s) 456, 1956
BspHI TCATGA 1 cut(s) 529
BspMAI CTGCAG 2 cut(s) 2494, 6453
BspMI ACCTGC 2 cut(s) 111, 1568
BspOI GCTAGC 1 cut(s) 3636
BspQI GCTCTTC 1 cut(s) 2143
BspT104I TTCGAA 1 cut(s) 2109
BspTNI GGTCTC 3 cut(s) 1727, 2935, 3404
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 4 cut(s) 1586, 4288, 4384, 6410
BsrFI RCCGGY 1 cut(s) 5146
BsrGI TGTACA 3 cut(s) 1849, 1963, 4777
BssAI RCCGGY 1 cut(s) 5146
BssNI GRCGYC 1 cut(s) 981
BssT1I CCWWGG 4 cut(s) 390, 4876, 5344, 5482
Bst2UI CCWGG 9 cut(s) 409, 1063, 2868, 3897, 4224, 4661, 4870, 5411, 6302
Bst6I CTCTTC 3 cut(s) 297, 2143, 3727
BstACI GRCGYC 1 cut(s) 981
BstAPI GCANNNNNTGC 1 cut(s) 4367
BstAUI TGTACA 3 cut(s) 1849, 1963, 4777
BstBAI YACGTR 4 cut(s) 1930, 3173, 3458, 3889
BstBI TTCGAA 1 cut(s) 2109
BstDSI CCRYGG 5 cut(s) 67, 2293, 3685, 4876, 5482
BstENI CCTNNNNNAGG 4 cut(s) 2382, 3584, 5173, 5565
BstFNI CGCG 2 cut(s) 456, 1956
BstHHI GCGC 5 cut(s) 456, 1204, 1752, 3022, 4395
BstMAI GTCTC 6 cut(s) 1727, 2935, 3116, 3404, 4000, 4154
BstMCI CGRYCG 1 cut(s) 1954
BstNI CCWGG 9 cut(s) 409, 1063, 2868, 3897, 4224, 4661, 4870, 5411, 6302
BstNSI RCATGY 7 cut(s) 101, 1004, 1750, 1964, 2179, 3139, 4778
BstSFI CTRYAG 2 cut(s) 2490, 6449
BstSLI GKGCMC 3 cut(s) 714, 1180, 5410
BstSNI TACGTA 2 cut(s) 3173, 3889
BstUI CGCG 2 cut(s) 456, 1956
BstV1I GCAGC 7 cut(s) 808, 1816, 2651, 3732, 3790, 5509, 6076
BstV2I GAAGAC 2 cut(s) 4326, 6347
BstX2I RGATCY 6 cut(s) 4075, 4404, 5953, 5965, 6252, 6286
BstXI CCANNNNNNTGG 4 cut(s) 2136, 4442, 5202, 5536
BstYI RGATCY 6 cut(s) 4075, 4404, 5953, 5965, 6252, 6286
Bsu15I ATCGAT 2 cut(s) 2857, 5834
BsuI GTATCC 2 cut(s) 675, 4957
BsuTUI ATCGAT 2 cut(s) 2857, 5834
BtgI CCRYGG 5 cut(s) 67, 2293, 3685, 4876, 5482
BtgZI GCGATG 2 cut(s) 3968, 4926
BtsI GCAGTG 4 cut(s) 964, 1176, 1284, 2499
BveI ACCTGC 2 cut(s) 111, 1568
CaiI CAGNNNCTG 4 cut(s) 2489, 2576, 4079, 6021
CciI TCATGA 1 cut(s) 529
CfoI GCGC 5 cut(s) 456, 1204, 1752, 3022, 4395
Cfr10I RCCGGY 1 cut(s) 5146
ClaI ATCGAT 2 cut(s) 2857, 5834
CseI GACGC 1 cut(s) 970
CspAI ACCGGT 1 cut(s) 5146
CspCI CAANNNNNGTGG 2 cut(s) 2305, 2340
DraI TTTAAA 2 cut(s) 2907, 4995
DraIII CACNNNGTG 1 cut(s) 3686
DrdI GACNNNNNNGTC 1 cut(s) 980
DriI GACNNNNNGTC 1 cut(s) 3369
DseDI GACNNNNNNGTC 1 cut(s) 980
EaeI YGGCCR 5 cut(s) 339, 2296, 3761, 6114, 6303
Eam1104I CTCTTC 3 cut(s) 297, 2143, 3727
Eam1105I GACNNNNNGTC 1 cut(s) 3369
EarI CTCTTC 3 cut(s) 297, 2143, 3727
Ecl136II GAGCTC 1 cut(s) 4237
Eco105I TACGTA 2 cut(s) 3173, 3889
Eco130I CCWWGG 4 cut(s) 390, 4876, 5344, 5482
Eco147I AGGCCT 1 cut(s) 6362
Eco24I GRGCYC 2 cut(s) 37, 4239
Eco31I GGTCTC 3 cut(s) 1727, 2935, 3404
Eco32I GATATC 5 cut(s) 348, 486, 994, 1120, 3010
Eco47I GGWCC 8 cut(s) 556, 1059, 1637, 2510, 2985, 4873, 5747, 5816
Eco53kI GAGCTC 1 cut(s) 4237
Eco72I CACGTG 2 cut(s) 1930, 3458
Eco88I CYCGRG 5 cut(s) 29, 1902, 3000, 4090, 6344
EcoICRI GAGCTC 1 cut(s) 4237
EcoNI CCTNNNNNAGG 4 cut(s) 2382, 3584, 5173, 5565
EcoO109I RGGNCCY 4 cut(s) 556, 1076, 2510, 2985
EcoRI GAATTC 2 cut(s) 1152, 1594
EcoRII CCWGG 9 cut(s) 407, 1061, 2866, 3895, 4222, 4659, 4868, 5409, 6300
EcoRV GATATC 5 cut(s) 348, 486, 994, 1120, 3010
EcoT14I CCWWGG 4 cut(s) 390, 4876, 5344, 5482
EcoT22I ATGCAT 4 cut(s) 938, 3240, 5167, 5261
EcoT38I GRGCYC 2 cut(s) 37, 4239
ErhI CCWWGG 4 cut(s) 390, 4876, 5344, 5482
FaqI GGGAC 8 cut(s) 101, 542, 1623, 2548, 4191, 4301, 4459, 4886
FauI CCCGC 1 cut(s) 5379
FauNDI CATATG 1 cut(s) 1627
FbaI TGATCA 1 cut(s) 1630
FblI GTMKAC 1 cut(s) 2030
FriOI GRGCYC 2 cut(s) 37, 4239
GlaI GCGC 5 cut(s) 455, 1203, 1751, 3021, 4394
GsaI CCCAGC 4 cut(s) 790, 2766, 3963, 4963
GsuI CTGGAG 5 cut(s) 976, 1713, 2379, 3324, 4031
HapII CCGG 4 cut(s) 754, 1037, 4634, 5147
HgaI GACGC 1 cut(s) 970
HhaI GCGC 5 cut(s) 456, 1204, 1752, 3022, 4395
Hin1I GRCGYC 1 cut(s) 981
Hin6I GCGC 5 cut(s) 454, 1202, 1750, 3020, 4393
HinP1I GCGC 5 cut(s) 454, 1202, 1750, 3020, 4393
HincII GTYRAC 4 cut(s) 1537, 1786, 2047, 5416
HindII GTYRAC 4 cut(s) 1537, 1786, 2047, 5416
HindIII AAGCTT 6 cut(s) 2281, 2352, 4117, 5555, 5735, 6158
HpaI GTTAAC 1 cut(s) 1537
HpaII CCGG 4 cut(s) 754, 1037, 4634, 5147
Hpy99I CGWCG 1 cut(s) 986
HpyCH4IV ACGT 6 cut(s) 1929, 3172, 3457, 3646, 3888, 6352
HpySE526I ACGT 6 cut(s) 1929, 3172, 3457, 3646, 3888, 6352
Hsp92I GRCGYC 1 cut(s) 981
HspAI GCGC 5 cut(s) 454, 1202, 1750, 3020, 4393
Ksp22I TGATCA 1 cut(s) 1630
KspAI GTTAAC 1 cut(s) 1537
LguI GCTCTTC 1 cut(s) 2143
LmnI GCTCC 7 cut(s) 40, 2257, 2527, 2974, 3850, 4662, 6463
Lsp1109I GCAGC 7 cut(s) 808, 1816, 2651, 3732, 3790, 5509, 6076
MaeII ACGT 6 cut(s) 1929, 3172, 3457, 3646, 3888, 6352
MaeIII GTNAC 9 cut(s) 309, 1460, 1834, 1889, 2221, 2485, 3131, 3651, 6040
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 3 cut(s) 2244, 6110, 6366
MflI RGATCY 6 cut(s) 4075, 4404, 5953, 5965, 6252, 6286
MhlI GDGCHC 7 cut(s) 37, 329, 438, 714, 1180, 4239, 5410
MlsI TGGCCA 2 cut(s) 2298, 6305
MluNI TGGCCA 2 cut(s) 2298, 6305
MlyI GAGTC 3 cut(s) 4630, 6105, 6128
MmeI TCCRAC 6 cut(s) 662, 1410, 2292, 3640, 3648, 6364
Mox20I TGGCCA 2 cut(s) 2298, 6305
Mph1103I ATGCAT 4 cut(s) 938, 3240, 5167, 5261
MroXI GAANNNNTTC 3 cut(s) 2193, 2444, 4203
MscI TGGCCA 2 cut(s) 2298, 6305
MslI CAYNNNNRTG 6 cut(s) 2379, 2662, 3460, 4440, 5060, 5423
Msp20I TGGCCA 2 cut(s) 2298, 6305
MspA1I CMGCKG 3 cut(s) 1358, 2473, 5660
MspI CCGG 4 cut(s) 754, 1037, 4634, 5147
MssI GTTTAAAC 1 cut(s) 4995
MunI CAATTG 3 cut(s) 2244, 6110, 6366
Mva1269I GAATGC 3 cut(s) 1390, 2773, 6149
MvaI CCWGG 9 cut(s) 409, 1063, 2868, 3897, 4224, 4661, 4870, 5411, 6302
MvnI CGCG 2 cut(s) 456, 1956
NciI CCSGG 2 cut(s) 754, 1038
NcoI CCATGG 2 cut(s) 4876, 5482
NdeI CATATG 1 cut(s) 1627
NheI GCTAGC 1 cut(s) 3632
NmeAIII GCCGAG 5 cut(s) 2255, 4883, 6035, 6142, 6216
NmuCI GTSAC 5 cut(s) 1889, 2221, 2485, 3131, 6040
NsiI ATGCAT 4 cut(s) 938, 3240, 5167, 5261
NspI RCATGY 7 cut(s) 101, 1004, 1750, 1964, 2179, 3139, 4778
NspV TTCGAA 1 cut(s) 2109
OliI CACNNNNGTG 1 cut(s) 3460
PaeI GCATGC 2 cut(s) 1004, 1750
PaeR7I CTCGAG 3 cut(s) 1902, 3000, 4090
PagI TCATGA 1 cut(s) 529
PaqCI CACCTGC 2 cut(s) 111, 1568
PasI CCCWGGG 1 cut(s) 4869
PceI AGGCCT 1 cut(s) 6362
PciSI GCTCTTC 1 cut(s) 2143
PctI GAATGC 3 cut(s) 1390, 2773, 6149
PdmI GAANNNNTTC 3 cut(s) 2193, 2444, 4203
PflFI GACNNNGTC 1 cut(s) 1790
PflMI CCANNNNNTGG 1 cut(s) 6021
PfoI TCCNGGA 1 cut(s) 4659
PinAI ACCGGT 1 cut(s) 5146
PleI GAGTC 3 cut(s) 4629, 6104, 6127
PmaCI CACGTG 2 cut(s) 1930, 3458
PmeI GTTTAAAC 1 cut(s) 4995
PmlI CACGTG 2 cut(s) 1930, 3458
PpsI GAGTC 3 cut(s) 4629, 6104, 6127
Ppu21I YACGTR 4 cut(s) 1930, 3173, 3458, 3889
PpuMI RGGWCCY 3 cut(s) 556, 2510, 2985
Psp124BI GAGCTC 1 cut(s) 4239
Psp5II RGGWCCY 3 cut(s) 556, 2510, 2985
Psp6I CCWGG 9 cut(s) 407, 1061, 2866, 3895, 4222, 4659, 4868, 5409, 6300
PspCI CACGTG 2 cut(s) 1930, 3458
PspFI CCCAGC 4 cut(s) 786, 2762, 3959, 4959
PspGI CCWGG 9 cut(s) 407, 1061, 2866, 3895, 4222, 4659, 4868, 5409, 6300
PspPPI RGGWCCY 3 cut(s) 556, 2510, 2985
PspXI VCTCGAGB 1 cut(s) 3000
PstI CTGCAG 2 cut(s) 2494, 6453
PstNI CAGNNNCTG 4 cut(s) 2489, 2576, 4079, 6021
PsuI RGATCY 6 cut(s) 4075, 4404, 5953, 5965, 6252, 6286
PsyI GACNNNGTC 1 cut(s) 1790
PvuII CAGCTG 1 cut(s) 1358
RseI CAYNNNNRTG 6 cut(s) 2379, 2662, 3460, 4440, 5060, 5423
SacI GAGCTC 1 cut(s) 4239
SapI GCTCTTC 1 cut(s) 2143
SchI GAGTC 3 cut(s) 4630, 6105, 6128
SduI GDGCHC 7 cut(s) 37, 329, 438, 714, 1180, 4239, 5410
SfcI CTRYAG 2 cut(s) 2490, 6449
Sfr274I CTCGAG 3 cut(s) 1902, 3000, 4090
SfuI TTCGAA 1 cut(s) 2109
SinI GGWCC 8 cut(s) 556, 1059, 1637, 2510, 2985, 4873, 5747, 5816
SlaI CTCGAG 3 cut(s) 1902, 3000, 4090
SmiI ATTTAAAT 1 cut(s) 2907
SmiMI CAYNNNNRTG 6 cut(s) 2379, 2662, 3460, 4440, 5060, 5423
SmlI CTYRAG 6 cut(s) 156, 1902, 3000, 4090, 4163, 5590
SmoI CTYRAG 6 cut(s) 156, 1902, 3000, 4090, 4163, 5590
SnaBI TACGTA 2 cut(s) 3173, 3889
SphI GCATGC 2 cut(s) 1004, 1750
SseBI AGGCCT 1 cut(s) 6362
SspI AATATT 6 cut(s) 1798, 2307, 5398, 5470, 5923, 6313
SstI GAGCTC 1 cut(s) 4239
StuI AGGCCT 1 cut(s) 6362
StyI CCWWGG 4 cut(s) 390, 4876, 5344, 5482
SwaI ATTTAAAT 1 cut(s) 2907
TaiI ACGT 6 cut(s) 1932, 3175, 3460, 3649, 3891, 6355
TaqII GACCGA 1 cut(s) 2959
TatI WGTACW 6 cut(s) 1849, 1963, 3754, 3778, 4070, 4777
TauI GCSGC 3 cut(s) 812, 2597, 6435
TseFI GTSAC 5 cut(s) 1889, 2221, 2485, 3131, 6040
TseI GCWGC 7 cut(s) 821, 1804, 2639, 3745, 3803, 5522, 6089
Tsp45I GTSAC 5 cut(s) 1889, 2221, 2485, 3131, 6040
TspGWI ACGGA 7 cut(s) 1416, 2128, 2282, 2708, 3674, 6341, 6450
Tth111I GACNNNGTC 1 cut(s) 1790
Van91I CCANNNNNTGG 1 cut(s) 6021
VpaK11BI GGWCC 8 cut(s) 556, 1059, 1637, 2510, 2985, 4873, 5747, 5816
XagI CCTNNNNNAGG 4 cut(s) 2382, 3584, 5173, 5565
XbaI TCTAGA 2 cut(s) 1346, 2552
XceI RCATGY 7 cut(s) 101, 1004, 1750, 1964, 2179, 3139, 4778
XcmI CCANNNNNNNNNTGG 2 cut(s) 1715, 6055
XhoI CTCGAG 3 cut(s) 1902, 3000, 4090
XmiI GTMKAC 1 cut(s) 2030
XmnI GAANNNNTTC 3 cut(s) 2193, 2444, 4203
Zsp2I ATGCAT 4 cut(s) 938, 3240, 5167, 5261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.