RchiOBHm_Chr6g0312731

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009462
Physical Location & Seq
Forward (+)
69299493 .. 69315428
15936 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28134

Sequence Viewer

Length: 6528 bp
ATGAGTGGAAAACTCCATTGCCCATTTCTCGGGGTTCCGATTCACAGCTCTTTAAGTGGTAGAAACCATGGGAATTTGATTCATTGGGACACAAGGCATGTGGGAAGAAGGGTTGTCCGCAGGTGTGTGAGTGAAAAGCAAAATTATTGGATTACTCAAGCAATGCGAGTTTCACATTTTTGGGGGAAAAATGTGGAGTTATTAAAGAGAACCTTTGAGCTGAAAAATGGCAAGATACAATGTGTTAAAGAGCCTTTTTCTCAAAGCAAGGCTCTGGTGAGGTCTTTATCTCCTCTGTGGGAGGAGGGGTTACTTTTAGTTAGGTGCTCTGTATTTGTTGCCGTGATATCTGGGGTATGCTTGTTGGTATGGTATGGGCAGAAAAAAGCCAAGGGTCTTATTGAAGCCAGGGTTTTGCCTTCTGTTTGCTCAGTACTCAGTGAGTATATTCAGCGCGAAATTGTTTTTGGTAAGGTTCGAAGGATATCACCTCTGAGCATCACATTGGAGGCGTGTTCAGTTGGCCCTCATAATGAGGAATTTTCTTGTGGTGAGGTCCCATCTATGAAACTTCTTTTTCGTCCTTTTGCAAGTCTGAGGAGGGGAAGGATTGTGATTGATGCAGTTTTGTCCCATCCAATTGTCTTGATTGCGCAGAAGAAGGATTATACATGGTTAGGGATACCCTCATCCGAAGGTAGTCTGCAGAGGCACTTATCAACAGAAGAAGGAATTGATCATCGTACAAAAACCCGGAGGCTAGCTAGAGAGGAAGCAGCTGATCGCTGGGAAAGAGCAAGGGATGAAGCAGCTAGAAAAGCTGCGGAGATGGGTTACATTATTTCAGACAAGGGTTCCACTCCATCCAAAGGTGATGATTCAAAGGAAGGTGATAGCCATTCAGGGGATTTAACAACCTCTGAATCGTTTTTATGCATGGATGAAAAGATGCACTGGAGAGATCACTGCATGGACACAGGTGTCGATTATGATATCAAACATGCAGACTTGGAGAAATCATTGGGTGTAAAAATACCGGGTTCTGGGCTTAAATTTTGGTCCAGAGTGATAAAGGGACCTAAAAAGCACAAATTCAAAAGGAAGGGCAACGGGAACGATATTTCTGCATCTGGTTTTACTGCCAAAAGAAGAATTCTTGGGGATAGTGCAGTAAGGGCACTTGCATATTTTCAGGATTTGGTGCATGGGAAGTCTGATGAACTTTCACAGGCATCCGGAAGTTATGATGTGATGAACCTTGACACCTATTTGATGAAGAATGAGGTTGCTAATAATGCCAACACTAGTGTCGCAGGTATTGGTCGAGAAACTGTCAGAGATGAAAACCGGAATGGAAAAGGTTCTAGAGATTCAGCAGACCACGCCCTTAAAGAAAATCAGAATGCAAACAGTCATTTAAGTAGTTTTAATCCCATACATGATCCATTAGATAGGTCCAATGTAGATGAGAAATCCAGTTACCCATCCACTGAAAATGTTTCAGAAACTAAAACCAGTTGTAATGTAAAAGATGAGGATTTTGGAAATGATGTTAAAAATAATCATTCTGAGGATGGTGAATCAGAAAGGCAAGCAGGTGAAACATTGCAGAATTCAACTTCCACAATACCCCCTTTTGCAACATATGATCATGTTCCCACCTGGCCCCTAAGTCAGAAATTAGGGATTCCCTCTTTTTCTAGAAATGCTGGAGTTCCAATATCCCATCTTCTCTCTGGTCTCATTCAAAAGCTGACATCTAGCATGCGCCCCAGAGTTGAAGATATTGTTGCAGAACTTGTCGACGAGGTCAATATTGTGCAGCCTGAAGGCATTGAGAAGATGCTTCCAGTTACACTGGATTCTGTACAATTCAAAGGAGGAACACTCATGCTACTTGCATACGGTGACAGGGAACCTCGAGAGATGGAGAATGTCAATGGACATGTGAAGTTTCAAAATCACTATGGTCGCGTGCATGTGCAAGTCAATGGCAACTGTAAGATGTGGAGGTCTGACATCATGTCTGAAGATGGTGGCTGGTTATCTGCAGATGTATTTGTTGACATTGTTGAACAGAAATGGCATGCGAACCTAAAAGTTGCTAACCTTTTTGCTCCGCTTTTTGAACGGATTTTAGAAATTCCATTAATGTGGTCCAAAGGAAGAGCCACTGGTGAGGTTCACTTGTGCATGTCAAGAGGAGAATCATTTCCCAATCTTCATGGACAGCTTGATGTGACAGGGTTGGCTTTTCAAACAATTGATGCTCCATCATCATTTTCTGACATCTCGGCAAGCTTATGTTTCCGTGGCCAGCGAATATTCTTACACAATGCAAGTGGCTGGTTTGGTGATGTTCCTTTGGAAGCGTCTGGAGATTTTGGTATTCATCCCGAGGAAGGAGAGTTTCATCTTATGTGTCAGGTTTCTTGCGTGGAAGTAAATGCCTTGATGAAAACTTTCAAGATGAAGCCTCTTATGTTTCCGCTGGCTGGTTCAGTGACTGCAGTGTTTAACTGTCAAGGTCCATTGGATGCTCCTATATTTGTGGGAAGTGGAATGGTATCTAGAAGGATGTCCCAGTCAGTTTCTGATTTTCCGCCATCTGCCGCATCAGAAGCAGTTTTGAAAAGTAAGGAAGCTGGTGCTGTGGCAGCGTTTGATCGTGTACCATTTTCATGCGTGTCTGCCAATTTCACTTTCAACACTGATAGCTGTGTCGCCGACTTGTATGGAATTAGAGCTAGTCTTGTCGATGGCGGTGAAATTCGAGGGGCTGGGAATGCATGGATTTGCCCAGAGGGTGAGGTTGATGATACATCAATGGATGTGAATTTCTCCGGAAGTATGTGCTTTGATAAAATTCTGCATCGATATATTCCTGGTTATCTTCAACTGATGCCACTGAAATTAGGGGATTTAAATGGAGAAACAAAACTTTCTGGATCCCTACTGAGACCGAGGTTTGACATTAAATGGACGGCTCCAAAGGCTGAAGGGTCCTTCAGCGATGCTCGAGGAGATATCATAATAGCGCATGATTCCATTACTATTAATTCCTCATCTACTGCCTTTGACTTGTCCTCAAAAGTTCTAACATCTTACAAGGATAAAGATTGGCTAAACAAAAGAGATGCTGAGACAAAGAGTGCCATGCCATTTGTTGTTGAGGGAATCGACTTGGATTTACGTATGCGTGGTTTTGAGTTCTTCAGCTTGGTATCTTCTTATCCCTTTGATTCTCCGAAACCCATGCATTTGAAAGCAACAGGGAAGATCAAGTTTCAGGGAAAGGTTTTAAAGCCTTTTAGTATTTCTAATGGACAAGAATTTGGTTCGGAGAGAAATAAGCAACAGGTGAATATGACAGATACAGGAAAGACAGATAGTCTCGTAGGAGAGGTTTCAATTTCAGGTCTCAAATTGAATCAGTTGATGTTGGCCCCTCAGTTGGCTGGATCGTTGAGCATTTCACGTGAGTGTATCAAGTTAGATGCCACAGGTAGGCCAGATGAAAGTCTTGTAGTGGAGTTTGTAGGGCCACTGAAGCCTAACAGTGAAACTAACACACAAAGTGGACAATTGTTGTCTTTCTTCCTTCAAAAGGGCCAACTAAAAGCAAACATTTGCTTCCAACCATTTCATTCTGCTAGCTTGGAGATACGTCAGTTACCACTCGATGAGTTGGAATTAGCTTCGCTCCGTGGAACAATACAAAAGGCAGAAATTCAGCTTAATCTTCAGAAACGAAGAGGTCATGGGCTGCTATCTGTACTTCGGCCAAAGTTTAGCGGTGTACTAGGTGAAGCCCTTGATGTGGCTGCTAGATGGAGTGGGGATGTTATCACTGTTGAGAAAACCATCTTGGAGCAAAGCAATAGTCGTTATGAGCTTCAGGGTGAATATGTATTGCCCGGCAGTCGAGATCGCAACCCTGCTGAGAAGGAAAGGGGAGGTTTATTGAAAAGAGCGATGGCTGGGAATCTGGGTAGTGTCATATCTTCTATGGGAAGGTGGAGAATGAGACTAGAAGTTCCTAGAGCTGAGGTTGCTGAGATGCTTCCCCTTGCCAGACTTGTTTCTCGAAGTACAGATCCCGCTGTTCACTCAAGATCAAAGGACCTGTTTGTTCAAAGCTTGCAGTCGGTTGGATTATATACAGAAAGTCTCAAAGAACTGCTTGAGGTTATACGGGGACATTATACCCCATTAAATGAAGTTATTCTGGAAGATGATCTTCCAGGTTTAACTGAGCTCAGAGGTAGCTGGCATGGCTCTCTTGATGCAAGCGGAGGTGGCAATGGGGACACAATGGCAGAATTTGATTTTCATGGGGATGACTGGGAGTGGGGAACCTACAAAACTCAGCGCGTTCTGGCAGTAGGTGCATACAGCAATGATGATGGTTTGCGCCTGGAAAAGATCTTTATTCAGAAAGACAACGCCACAGTCCATGCAGATGGGACTTTGTTAGGGCCGAAAACAAATCTACACTTTGCTGTTTTGAACTTTCCTGTTAGTCTAGTTCCCACTGTGATTCAGGTCATTGAATCTTCTGCTACTGATGCTGTTCAATCTCTACGGCAATTTTTAGCACCAATTAGAGGCATACTGCACATGGAAGGCGATCTTAGAGGGAGTCTTGCGAAACCAGAATGTGATGTGCAAGTAAGGCTCCTGGATGGTGCCGTTGGGGGGATTGATCTCGGACGAGCTGAAATTGTTGCTTCCCTCACCTCAACCAGTCGTTTTCTGTTCAATGCAAAATTTGAACCAATCATCCAAACTGGGCATGTACACATTCAGGGAAGCATACCTGTTACTTTTGTTCAGAATAACATGTTAGAGGAAGAAGATTTAGAAAAAGATAAAAGCCGAGCTACTTCCTGGGACCATGGTTGGGTGAAGGAAAGAGGCAGGGGGTCTAGCGATGATGCCAGTGAAAAGAAACTTCCAAGAGAGAGGAATGAAGAAGGCTGGGATACCGGATTAGCTGAAAGCCTTAAAGGTTTAAACTGGAACATATTAGATGTAGGGGAAGTCAGAATAGATGCTGATATAAAAGATGGGGGCATGATGATGTTAACTGCTTTATCTCCTTATGCTAAATGGCTTCAGGGCAATGCCGACATCATGCTTCAGGTCAGAGGCACAGTTGAACAACCAGTGCTGGATGGATATGCATCCTTCCACAGGGCATCTATATCATCACCGGTTCTCTGGAAACCACTTACAAATTTCGGTGGCACAGTTCATGTGAAATCAAATAGGTTATGCATCACTTCATTGGAGAGTAGAGTAAGTAGAAGGGGTAAGCTGTTTGTCAAGGGAAACCTGCCCCTTAGAACAAGTGAAGCATCCCTTGGAGATAAAATTGACTTGAAATGTGAAGTTCTGGAAGTGCGGGCAAAAAATATTTTAAGTGCCCAGGTTGACACTCAGATGCAGATAACTGGTTCCATATTGCAACCAAACATCTCTGGAAGTATTAAATTGAGCCATGGTGAAGCATATTTGCCACACGATAAGGGTAGTGGGGCTGCCCCCAATAGATTGGCAACAAATGAACCTAGGCTTCCTAGTACAGGTGTTGATCGAGCAGTTGCCTCGAGATATGTTTCACGGTTTTTCAGTTCACAACCTGCTGCTTCATGGACAAAATTCCCTCAACCTTCAGCGGTTAAGCAAGCAGTACAGGAGATGGAGCAAGTGAACATTAAACCGAATGTAGATATTCAACTTAGTGATTTGAAGCTTGTTCTAGGACCAGAGCTGAGGATTGTATATCCTTTAATTCTAAATTTTGCTGTTAGTGGAGAGCTAGAGTTAAATGGTCCAGCTCATCCCAAATCGATAAAACCTAGAGGCATCTTAACTTTTGAAAATGGTGATGTCAATCTTGTCGCTACTCAGGTGAGGCTCAGGCAAGAGCATCTGAATATTGCAAAGTTTGAGCCTGAACATGGATTAGATCCAATGCTAGATCTAGTTTTAGTGGGGTCTGAGTGGCAATTCAGGATTCAAAGTCGAGCCAGCAACTGGCAGGAAAAACTGGTTGTGACCTCCACCCGTTCTGTGGAACAGGACGCTCTCTCACCTACTGAGGCTGCTAGAGTCTTTGAAAGTCAATTGGCAGAGTCCATCTTGGAAGGTGATGGGCAGCTTGCATTTCAAAAGCTTGCAACTACAACACTTGAAAAACTAATGCCGAGGATAGAAGGGAAGGGAGAGTTTGGTCAGGCAAGGTGGAGGCTAGTGTATGCACCACAGATCCCTAGCTTGCTTTCTGTTGATCCTACTGTGGATCCTCTGAAATCCTTGGCCAGTAATATTTCATTTGGTACAGAAGTTGAAGTCCAACTTGGGAAACGTCTTCAGGCCTCAATTGTTCGACAAATGAAGGACTCGGAAATGGCAATGCAATGGACATTGATCTACCAGCTTACCAGCCGCCTGCGTGTTCTGCTACAGTCTGCTCCTTCAAAACGCCTCATTTTTGAATATTCTGCAACATCTCAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TamB

Protein Analysis

2175

Amino Acids

240.36

Weight (kDa)

6.49

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TamB PF04357 1764 - 2158 2.7e-15 TamB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 111, 1586
AasI GACNNNNNNGTC 1 cut(s) 980
Acc16I TGCGCA 1 cut(s) 654
Acc36I ACCTGC 5 cut(s) 111, 1304, 1586, 5346, 5653
AccB1I GGYRCC 1 cut(s) 4688
AccB7I CCANNNNNTGG 1 cut(s) 6042
AccI GTMKAC 1 cut(s) 1803
AccII CGCG 3 cut(s) 456, 1974, 4374
AccIII TCCGGA 2 cut(s) 1235, 2843
AcoI YGGCCR 3 cut(s) 2314, 3782, 6324
AcvI CACGTG 1 cut(s) 3479
AdeI CACNNNGTG 1 cut(s) 3578
AflIII ACRYGT 2 cut(s) 1945, 4842
AgeI ACCGGT 1 cut(s) 5215
AhdI GACNNNNNGTC 2 cut(s) 2023, 3390
AhlI ACTAGT 1 cut(s) 1304
AjnI CCWGG 8 cut(s) 407, 1661, 2884, 4243, 4416, 4680, 4889, 5430
AleI CACNNNNGTG 1 cut(s) 3481
AloI GAACNNNNNNTCC 2 cut(s) 4767, 4799
Alw21I GWGCWC 2 cut(s) 329, 4260
Alw26I GTCTC 7 cut(s) 1745, 2953, 3137, 3398, 3425, 4021, 4175
AlwNI CAGNNNCTG 3 cut(s) 2507, 2594, 6042
Ama87I CYCGRG 5 cut(s) 29, 1920, 2396, 3018, 5611
Aor13HI TCCGGA 2 cut(s) 1235, 2843
ArsI GACNNNNNNTTYG 2 cut(s) 3868, 3900
AseI ATTAAT 2 cut(s) 2150, 3057
AsiGI ACCGGT 1 cut(s) 5215
Asp700I GAANNNNTTC 4 cut(s) 1360, 2211, 2462, 4224
AspA2I CCTAGG 1 cut(s) 5573
AspLEI GCGC 6 cut(s) 456, 655, 1770, 3040, 4374, 4416
AsuC2I CCSGG 3 cut(s) 754, 1038, 3918
AsuII TTCGAA 1 cut(s) 478
AsuNHI GCTAGC 2 cut(s) 760, 3653
AvaI CYCGRG 5 cut(s) 29, 1920, 2396, 3018, 5611
AvrII CCTAGG 1 cut(s) 5573
BaeGI GKGCMC 2 cut(s) 1180, 5431
BaeI ACNNNNGTAYC 4 cut(s) 3656, 3689, 6336, 6369
BalI TGGCCA 2 cut(s) 2316, 6326
BamHI GGATCC 2 cut(s) 2948, 6307
BanI GGYRCC 1 cut(s) 4688
BanII GRGCYC 1 cut(s) 4260
BbrPI CACGTG 1 cut(s) 3479
BbsI GAAGAC 1 cut(s) 6368
Bbv12I GWGCWC 2 cut(s) 329, 4260
BbvCI CCTCAGC 2 cut(s) 4047, 5777
BceAI ACGGC 4 cut(s) 326, 3000, 4601, 4676
BcgI CGANNNNNNTGC 6 cut(s) 1106, 1140, 3238, 3272, 5592, 5626
BciT130I CCWGG 8 cut(s) 409, 1663, 2886, 4245, 4418, 4682, 4891, 5432
BciVI GTATCC 2 cut(s) 675, 4978
BclI TGATCA 2 cut(s) 736, 1648
BcnI CCSGG 3 cut(s) 754, 1038, 3918
BcoDI GTCTC 7 cut(s) 1745, 2953, 3137, 3398, 3425, 4021, 4175
BcuI ACTAGT 1 cut(s) 1304
BfmI CTRYAG 4 cut(s) 704, 2049, 2508, 6470
BfuAI ACCTGC 5 cut(s) 111, 1304, 1586, 5346, 5653
BfuI GTATCC 2 cut(s) 675, 4978
BglII AGATCT 2 cut(s) 4425, 5986
BlnI CCTAGG 1 cut(s) 5573
BmcAI AGTACT 1 cut(s) 435
BmeRI GACNNNNNGTC 2 cut(s) 2023, 3390
BmeT110I CYCGRG 5 cut(s) 29, 1920, 2396, 3018, 5611
BmrI ACTGGG 3 cut(s) 2578, 4354, 4800
BmtI GCTAGC 2 cut(s) 764, 3657
BmuI ACTGGG 3 cut(s) 2578, 4354, 4800
BpiI GAAGAC 1 cut(s) 6368
BplI GAGNNNNNCTC 2 cut(s) 1872, 1904
BpmI CTGGAG 3 cut(s) 976, 1731, 2397
Bpu10I CCTNAGC 3 cut(s) 4047, 5777, 5924
Bpu14I TTCGAA 1 cut(s) 478
BpuEI CTTGAG 3 cut(s) 141, 4096, 4205
BpuMI CCSGG 3 cut(s) 754, 1038, 3918
Bsa29I ATCGAT 2 cut(s) 2875, 5855
BsaAI YACGTR 2 cut(s) 3194, 3479
BsaBI GATNNNNATC 2 cut(s) 5185, 5898
BsaI GGTCTC 3 cut(s) 1745, 2953, 3425
BsaWI WCCGGW 5 cut(s) 1235, 1347, 2843, 4988, 5215
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bse118I RCCGGY 1 cut(s) 5215
Bse3DI GCAATG 8 cut(s) 16, 168, 1604, 4309, 4405, 5131, 6426, 6431
Bse8I GATNNNNATC 2 cut(s) 5185, 5898
BseAI TCCGGA 2 cut(s) 1235, 2843
BseBI CCWGG 8 cut(s) 409, 1663, 2886, 4245, 4418, 4682, 4891, 5432
BseCI ATCGAT 2 cut(s) 2875, 5855
BseJI GATNNNNATC 2 cut(s) 5185, 5898
BseMI GCAATG 8 cut(s) 16, 168, 1604, 4309, 4405, 5131, 6426, 6431
BseRI GAGGAG 5 cut(s) 282, 317, 613, 2217, 3036
BseSI GKGCMC 2 cut(s) 1180, 5431
BseYI CCCAGC 4 cut(s) 786, 2780, 3980, 4980
BsgI GTGCAG 3 cut(s) 1188, 1841, 4601
Bsh1236I CGCG 3 cut(s) 456, 1974, 4374
BshNI GGYRCC 1 cut(s) 4688
BshTI ACCGGT 1 cut(s) 5215
BshVI ATCGAT 2 cut(s) 2875, 5855
BsiHKAI GWGCWC 2 cut(s) 329, 4260
BsiHKCI CYCGRG 5 cut(s) 29, 1920, 2396, 3018, 5611
BsiSI CCGG 8 cut(s) 754, 1037, 1236, 1348, 2844, 3918, 4989, 5216
BslFI GGGAC 9 cut(s) 101, 542, 616, 1089, 2566, 4212, 4322, 4480, 4907
BsmAI GTCTC 7 cut(s) 1745, 2953, 3137, 3398, 3425, 4021, 4175
BsmFI GGGAC 9 cut(s) 101, 542, 616, 1089, 2566, 4212, 4322, 4480, 4907
BsmI GAATGC 2 cut(s) 1408, 2791
Bso31I GGTCTC 3 cut(s) 1745, 2953, 3425
BsoBI CYCGRG 5 cut(s) 29, 1920, 2396, 3018, 5611
Bsp119I TTCGAA 1 cut(s) 478
Bsp1286I GDGCHC 4 cut(s) 329, 1180, 4260, 5431
Bsp13I TCCGGA 2 cut(s) 1235, 2843
Bsp1407I TGTACA 2 cut(s) 1867, 4798
Bsp19I CCATGG 3 cut(s) 67, 4897, 5503
BspDI ATCGAT 2 cut(s) 2875, 5855
BspEI TCCGGA 2 cut(s) 1235, 2843
BspFNI CGCG 3 cut(s) 456, 1974, 4374
BspMAI CTGCAG 3 cut(s) 708, 2053, 2512
BspMI ACCTGC 5 cut(s) 111, 1304, 1586, 5346, 5653
BspOI GCTAGC 2 cut(s) 764, 3657
BspQI GCTCTTC 1 cut(s) 2161
BspT104I TTCGAA 1 cut(s) 478
BspT107I GGYRCC 1 cut(s) 4688
BspTNI GGTCTC 3 cut(s) 1745, 2953, 3425
BsrDI GCAATG 8 cut(s) 16, 168, 1604, 4309, 4405, 5131, 6426, 6431
BsrFI RCCGGY 1 cut(s) 5215
BsrGI TGTACA 2 cut(s) 1867, 4798
BssAI RCCGGY 1 cut(s) 5215
BssT1I CCWWGG 7 cut(s) 67, 390, 4897, 5365, 5503, 5573, 6321
Bst2UI CCWGG 8 cut(s) 409, 1663, 2886, 4245, 4418, 4682, 4891, 5432
Bst6I CTCTTC 2 cut(s) 2161, 3748
BstAPI GCANNNNNTGC 1 cut(s) 4388
BstAUI TGTACA 2 cut(s) 1867, 4798
BstBAI YACGTR 2 cut(s) 3194, 3479
BstBI TTCGAA 1 cut(s) 478
BstDSI CCRYGG 5 cut(s) 67, 2311, 3706, 4897, 5503
BstENI CCTNNNNNAGG 3 cut(s) 3605, 5194, 5586
BstFNI CGCG 3 cut(s) 456, 1974, 4374
BstHHI GCGC 6 cut(s) 456, 655, 1770, 3040, 4374, 4416
BstMAI GTCTC 7 cut(s) 1745, 2953, 3137, 3398, 3425, 4021, 4175
BstNI CCWGG 8 cut(s) 409, 1663, 2886, 4245, 4418, 4682, 4891, 5432
BstNSI RCATGY 9 cut(s) 101, 1004, 1768, 1949, 1982, 2090, 2197, 4799, 4846
BstSFI CTRYAG 4 cut(s) 704, 2049, 2508, 6470
BstSLI GKGCMC 2 cut(s) 1180, 5431
BstSNI TACGTA 1 cut(s) 3194
BstUI CGCG 3 cut(s) 456, 1974, 4374
BstV2I GAAGAC 1 cut(s) 6368
BstX2I RGATCY 7 cut(s) 2948, 4096, 4425, 5974, 5986, 6273, 6307
BstXI CCANNNNNNTGG 3 cut(s) 2154, 4463, 5557
BstYI RGATCY 7 cut(s) 2948, 4096, 4425, 5974, 5986, 6273, 6307
Bsu15I ATCGAT 2 cut(s) 2875, 5855
BsuI GTATCC 2 cut(s) 675, 4978
BsuTUI ATCGAT 2 cut(s) 2875, 5855
BtgI CCRYGG 5 cut(s) 67, 2311, 3706, 4897, 5503
BtgZI GCGATG 3 cut(s) 3027, 3989, 4947
BtsI GCAGTG 2 cut(s) 964, 2517
BveI ACCTGC 5 cut(s) 111, 1304, 1586, 5346, 5653
CaiI CAGNNNCTG 3 cut(s) 2507, 2594, 6042
CfoI GCGC 6 cut(s) 456, 655, 1770, 3040, 4374, 4416
Cfr10I RCCGGY 1 cut(s) 5215
ClaI ATCGAT 2 cut(s) 2875, 5855
CseI GACGC 2 cut(s) 2361, 6098
CspAI ACCGGT 1 cut(s) 5215
CspCI CAANNNNNGTGG 4 cut(s) 81, 116, 2323, 2358
DraI TTTAAA 3 cut(s) 2925, 3303, 5016
DraIII CACNNNGTG 1 cut(s) 3578
DrdI GACNNNNNNGTC 1 cut(s) 980
DriI GACNNNNNGTC 2 cut(s) 2023, 3390
DseDI GACNNNNNNGTC 1 cut(s) 980
EaeI YGGCCR 3 cut(s) 2314, 3782, 6324
Eam1104I CTCTTC 2 cut(s) 2161, 3748
Eam1105I GACNNNNNGTC 2 cut(s) 2023, 3390
EarI CTCTTC 2 cut(s) 2161, 3748
EciI GGCGGA 1 cut(s) 2592
Ecl136II GAGCTC 1 cut(s) 4258
Eco105I TACGTA 1 cut(s) 3194
Eco130I CCWWGG 7 cut(s) 67, 390, 4897, 5365, 5503, 5573, 6321
Eco147I AGGCCT 1 cut(s) 6383
Eco24I GRGCYC 1 cut(s) 4260
Eco31I GGTCTC 3 cut(s) 1745, 2953, 3425
Eco32I GATATC 4 cut(s) 348, 486, 994, 3028
Eco53kI GAGCTC 1 cut(s) 4258
Eco72I CACGTG 1 cut(s) 3479
Eco88I CYCGRG 5 cut(s) 29, 1920, 2396, 3018, 5611
EcoICRI GAGCTC 1 cut(s) 4258
EcoNI CCTNNNNNAGG 3 cut(s) 3605, 5194, 5586
EcoO109I RGGNCCY 4 cut(s) 556, 1076, 3003, 4123
EcoRI GAATTC 2 cut(s) 1152, 1612
EcoRII CCWGG 8 cut(s) 407, 1661, 2884, 4243, 4416, 4680, 4889, 5430
EcoRV GATATC 4 cut(s) 348, 486, 994, 3028
EcoT14I CCWWGG 7 cut(s) 67, 390, 4897, 5365, 5503, 5573, 6321
EcoT22I ATGCAT 5 cut(s) 938, 2791, 3261, 5188, 5282
EcoT38I GRGCYC 1 cut(s) 4260
ErhI CCWWGG 7 cut(s) 67, 390, 4897, 5365, 5503, 5573, 6321
FaqI GGGAC 9 cut(s) 101, 542, 616, 1089, 2566, 4212, 4322, 4480, 4907
FauI CCCGC 2 cut(s) 4108, 5400
FauNDI CATATG 1 cut(s) 1645
FbaI TGATCA 2 cut(s) 736, 1648
FblI GTMKAC 1 cut(s) 1803
FriOI GRGCYC 1 cut(s) 4260
FspI TGCGCA 1 cut(s) 654
GlaI GCGC 6 cut(s) 455, 654, 1769, 3039, 4373, 4415
GsaI CCCAGC 4 cut(s) 790, 2784, 3984, 4984
GsuI CTGGAG 3 cut(s) 976, 1731, 2397
HapII CCGG 8 cut(s) 754, 1037, 1236, 1348, 2844, 3918, 4989, 5216
HgaI GACGC 2 cut(s) 2361, 6098
HhaI GCGC 6 cut(s) 456, 655, 1770, 3040, 4374, 4416
Hin6I GCGC 6 cut(s) 454, 653, 1768, 3038, 4372, 4414
HinP1I GCGC 6 cut(s) 454, 653, 1768, 3038, 4372, 4414
HincII GTYRAC 4 cut(s) 1804, 2065, 5088, 5437
HindII GTYRAC 4 cut(s) 1804, 2065, 5088, 5437
HindIII AAGCTT 4 cut(s) 2299, 4138, 5756, 6179
HpaI GTTAAC 1 cut(s) 5088
HpaII CCGG 8 cut(s) 754, 1037, 1236, 1348, 2844, 3918, 4989, 5216
Hpy99I CGWCG 1 cut(s) 1808
HpyCH4IV ACGT 4 cut(s) 3193, 3478, 3667, 6373
HpySE526I ACGT 4 cut(s) 3193, 3478, 3667, 6373
HspAI GCGC 6 cut(s) 454, 653, 1768, 3038, 4372, 4414
Kpn2I TCCGGA 2 cut(s) 1235, 2843
Ksp22I TGATCA 2 cut(s) 736, 1648
KspAI GTTAAC 1 cut(s) 5088
LguI GCTCTTC 1 cut(s) 2161
LmnI GCTCC 9 cut(s) 2122, 2275, 2545, 2992, 3708, 3871, 4683, 5707, 6484
MaeII ACGT 4 cut(s) 3193, 3478, 3667, 6373
MfeI CAATTG 5 cut(s) 639, 2262, 3584, 6131, 6387
MflI RGATCY 7 cut(s) 2948, 4096, 4425, 5974, 5986, 6273, 6307
MhlI GDGCHC 4 cut(s) 329, 1180, 4260, 5431
MlsI TGGCCA 2 cut(s) 2316, 6326
MluNI TGGCCA 2 cut(s) 2316, 6326
MlyI GAGTC 4 cut(s) 4651, 6126, 6149, 6401
MmeI TCCRAC 4 cut(s) 3661, 3669, 4132, 6385
Mox20I TGGCCA 2 cut(s) 2316, 6326
Mph1103I ATGCAT 5 cut(s) 938, 2791, 3261, 5188, 5282
MroI TCCGGA 2 cut(s) 1235, 2843
MroXI GAANNNNTTC 4 cut(s) 1360, 2211, 2462, 4224
MscI TGGCCA 2 cut(s) 2316, 6326
MslI CAYNNNNRTG 7 cut(s) 2152, 2680, 3481, 4338, 4461, 5081, 5444
Msp20I TGGCCA 2 cut(s) 2316, 6326
MspA1I CMGCKG 4 cut(s) 779, 2491, 4103, 5681
MspI CCGG 8 cut(s) 754, 1037, 1236, 1348, 2844, 3918, 4989, 5216
MssI GTTTAAAC 1 cut(s) 5016
MunI CAATTG 5 cut(s) 639, 2262, 3584, 6131, 6387
Mva1269I GAATGC 2 cut(s) 1408, 2791
MvaI CCWGG 8 cut(s) 409, 1663, 2886, 4245, 4418, 4682, 4891, 5432
MvnI CGCG 3 cut(s) 456, 1974, 4374
NciI CCSGG 3 cut(s) 754, 1038, 3918
NcoI CCATGG 3 cut(s) 67, 4897, 5503
NdeI CATATG 1 cut(s) 1645
NheI GCTAGC 2 cut(s) 760, 3653
NmeAIII GCCGAG 3 cut(s) 2273, 4904, 6237
NmuCI GTSAC 4 cut(s) 1907, 2239, 2503, 6061
NsbI TGCGCA 1 cut(s) 654
NsiI ATGCAT 5 cut(s) 938, 2791, 3261, 5188, 5282
NspI RCATGY 9 cut(s) 101, 1004, 1768, 1949, 1982, 2090, 2197, 4799, 4846
NspV TTCGAA 1 cut(s) 478
OliI CACNNNNGTG 1 cut(s) 3481
PaeI GCATGC 2 cut(s) 1768, 2090
PaeR7I CTCGAG 3 cut(s) 1920, 3018, 5611
PaqCI CACCTGC 2 cut(s) 111, 1586
PceI AGGCCT 1 cut(s) 6383
PciI ACATGT 2 cut(s) 1945, 4842
PciSI GCTCTTC 1 cut(s) 2161
PctI GAATGC 2 cut(s) 1408, 2791
PdmI GAANNNNTTC 4 cut(s) 1360, 2211, 2462, 4224
PflFI GACNNNGTC 1 cut(s) 1808
PflMI CCANNNNNTGG 1 cut(s) 6042
PfoI TCCNGGA 1 cut(s) 4680
PinAI ACCGGT 1 cut(s) 5215
PleI GAGTC 4 cut(s) 4650, 6125, 6148, 6401
PmaCI CACGTG 1 cut(s) 3479
PmeI GTTTAAAC 1 cut(s) 5016
PmlI CACGTG 1 cut(s) 3479
PpsI GAGTC 4 cut(s) 4650, 6125, 6148, 6401
Ppu21I YACGTR 2 cut(s) 3194, 3479
PpuMI RGGWCCY 4 cut(s) 556, 1076, 3003, 4123
PscI ACATGT 2 cut(s) 1945, 4842
PshBI ATTAAT 2 cut(s) 2150, 3057
Psp124BI GAGCTC 1 cut(s) 4260
Psp5II RGGWCCY 4 cut(s) 556, 1076, 3003, 4123
Psp6I CCWGG 8 cut(s) 407, 1661, 2884, 4243, 4416, 4680, 4889, 5430
PspCI CACGTG 1 cut(s) 3479
PspFI CCCAGC 4 cut(s) 786, 2780, 3980, 4980
PspGI CCWGG 8 cut(s) 407, 1661, 2884, 4243, 4416, 4680, 4889, 5430
PspPPI RGGWCCY 4 cut(s) 556, 1076, 3003, 4123
PspXI VCTCGAGB 1 cut(s) 3018
PstI CTGCAG 3 cut(s) 708, 2053, 2512
PstNI CAGNNNCTG 3 cut(s) 2507, 2594, 6042
PsuI RGATCY 7 cut(s) 2948, 4096, 4425, 5974, 5986, 6273, 6307
PsyI GACNNNGTC 1 cut(s) 1808
PvuII CAGCTG 1 cut(s) 779
RseI CAYNNNNRTG 7 cut(s) 2152, 2680, 3481, 4338, 4461, 5081, 5444
SacI GAGCTC 1 cut(s) 4260
SalI GTCGAC 1 cut(s) 1802
SapI GCTCTTC 1 cut(s) 2161
ScaI AGTACT 1 cut(s) 435
SchI GAGTC 4 cut(s) 4651, 6126, 6149, 6401
SduI GDGCHC 4 cut(s) 329, 1180, 4260, 5431
SfcI CTRYAG 4 cut(s) 704, 2049, 2508, 6470
Sfr274I CTCGAG 3 cut(s) 1920, 3018, 5611
SfuI TTCGAA 1 cut(s) 478
SlaI CTCGAG 3 cut(s) 1920, 3018, 5611
SmiI ATTTAAAT 1 cut(s) 2925
SmiMI CAYNNNNRTG 7 cut(s) 2152, 2680, 3481, 4338, 4461, 5081, 5444
SmlI CTYRAG 6 cut(s) 156, 1920, 3018, 4111, 4184, 5611
SmoI CTYRAG 6 cut(s) 156, 1920, 3018, 4111, 4184, 5611
SnaBI TACGTA 1 cut(s) 3194
SpeI ACTAGT 1 cut(s) 1304
SphI GCATGC 2 cut(s) 1768, 2090
SseBI AGGCCT 1 cut(s) 6383
SspI AATATT 6 cut(s) 1816, 2325, 5419, 5944, 6334, 6506
SstI GAGCTC 1 cut(s) 4260
StuI AGGCCT 1 cut(s) 6383
StyI CCWWGG 7 cut(s) 67, 390, 4897, 5365, 5503, 5573, 6321
SwaI ATTTAAAT 1 cut(s) 2925
TaiI ACGT 4 cut(s) 3196, 3481, 3670, 6376
TaqII GACCGA 1 cut(s) 2977
TatI WGTACW 8 cut(s) 433, 1867, 3775, 3799, 4091, 4798, 5585, 5695
TauI GCSGC 2 cut(s) 2615, 6456
TseFI GTSAC 4 cut(s) 1907, 2239, 2503, 6061
Tsp45I GTSAC 4 cut(s) 1907, 2239, 2503, 6061
TspGWI ACGGA 3 cut(s) 2146, 2300, 3695
Tth111I GACNNNGTC 1 cut(s) 1808
Van91I CCANNNNNTGG 1 cut(s) 6042
VspI ATTAAT 2 cut(s) 2150, 3057
XagI CCTNNNNNAGG 3 cut(s) 3605, 5194, 5586
XbaI TCTAGA 3 cut(s) 1364, 1700, 2570
XceI RCATGY 9 cut(s) 101, 1004, 1768, 1949, 1982, 2090, 2197, 4799, 4846
XcmI CCANNNNNNNNNTGG 2 cut(s) 1733, 6076
XhoI CTCGAG 3 cut(s) 1920, 3018, 5611
XmaJI CCTAGG 1 cut(s) 5573
XmiI GTMKAC 1 cut(s) 1803
XmnI GAANNNNTTC 4 cut(s) 1360, 2211, 2462, 4224
ZrmI AGTACT 1 cut(s) 435
Zsp2I ATGCAT 5 cut(s) 938, 2791, 3261, 5188, 5282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.