MD08G1135800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009462
Physical Location & Seq
Forward (+)
12908031 .. 12927680
19650 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1135800.v1.1.491

Sequence Viewer

Length: 6513 bp
ATGAGCAGTAAACTCCTCTGCCCATTTATTGGGGTTTCACTTCACGACTCTTTAAATGGTAGAAACAATGGAAATTTCATTTATTGGGACAGAGGGCGTACGGGAAAAAGGGCTGCTCGTAGGTGTGTATGTGAAAAGCAGAACTATTGGATTTCTCAAGCAATTAAAGTTTCACATTTTTGGGGAAAAAATGTGGAATTATTAAGGCGAACCTTCGAGCTAAAAAATGGGATGAAGGTGCAATGTGTTAAAGAGCCCTTTTCTCGAAGCAAGGCTCTGGTGAGGTCTCTGTCTCCTCTGTTGGAAGAGGGGTTACTTTTAATTAGGTGCTCTGTACTTTTGGCTGTCATATCTGGGGTGTGCTTATTGGTATGGTACGGGCAATCCAAAACCAAGGATTTTATTGAAGCCAAGCTTTTGCCTTCTGTTTGTTCAGTACTCAGTGAATATGTTGAGCGTGAAGTTGTTTTTGGTAAGGTTCGGAGGTTATCGCCTTTGAGCATCACACTGGACTCTTGTTCAATTGGGCCTCATAATGAGGAATTTTCTTGTGGTGAGGTCCCATCTATGAAACTTCGGCTTCATCCTTTTGCAAGTCTTAGGAGGGGAAGGATTGTGATTGATGCGGTTTTGTCGCATCCGACGGTCTTGGTTGCACAAAAGAAGGATTATACTTGGTTAGGGATACCCTCAACTGAAGGTGGTCTACAAAGGCACTTATCTAACGAAGAAGGAATTGATCATCGAACCAAAATCCGAAGGCTTTCTAGAGAAGAAGCGGCTGCTTGCTGGGAAAGAGAAAGAGATGAAGCGGCAAAAAAAGCTGCAGAGATGGGTTACATTGTTTCTGACAAGGGTTCCAGTCCTTCAAATGGCAATGACTCAAAGGAAGGTGATAGCCATTCAGTAGATTTAACAAGTTCTGAGTCATTTCCATGCATGGATGAAAAGATGCACTGGAGGGATCATTGCATGGACACAGGCGTTGATTATGAAATAAAGCATGCAGATTTGGAGAAATCGTTAGGTGTCAAAATTCCTGGGTCAGGGCTTAAATTTTGGTCCAGAGTGATAAAGGGGCCTAAAAAGCACAAATTTAAAAGGAAGGGTTATGGGAGTGATATCTCTGCATCCGGTATTACTGCCAAAAGAAGAATTCTTGGGTACAGTGCAGCAAGGGCACTTGCATATTTTCAGGATCTATCTCATCGGAAGTCTGACGAGCCTTTACAGTCATCTGGAGTTTATGATGTGATGAACCTTGACACCTATTTGTTGAACAATGTGGTTGATACTAATGCTGATGCCTCCATCACAAGTATTGGTGAAAAAACTGTAAGAGATGACAACCACAATGGAAAGCATTACGGAGATTCAGCAGACTATCCTCTTAAAGAAAATGAGAATGTCAACAGTCATTTAAATAGTTCTAATTTCATGGATGATCCGCTTCCTATGATTTTTGATAGGTCTAATGGAGATGGGATGTCCAGTAAAACTTTCCCATTCACTGAAAATGTTGCAGGAGCTAAAACCATAAGTGGTAATGTAAACGATGAGGATTTTGGAGTCGATGTTGTAAATAAGCATACTGAGATATCAGAGAGGCAAAGAGGTCAGATGCTGCAGACTTCAAATTTTGCAACATACAATCAAGTTCCTACATGGCCCCTAAGCCTGAAATTAGGTTTTCCCCCCTTTGCAGGAGAGCCATTAGCCTTTCTCTCTGGACCCATTCAAAAGCTGACGTCTAGTGTGGGCCCCAGAGTTGAAGATATTGTTGCAGAACTTGTCGACGGGGTCGGTGTTGGGCAGTCTGAAGGCATTGAGAAGATGCTTCCAGTTACACTAGATTCTGTTCATTTCAAAGGAGGAACATTGATGCTACTTGCATATGGCGACAGGGAACCTCGAGAGATGGAGAATGTCGATGGACATGTGAAGTTTCAAAATCACTATGGCCAAGTTCACGTACAAGTTAGTGGAAACTGTAAGGTGTGGAGGTCTGATAACATATCTGAAGATGGCGGCTGGTTGTCTACAGATGTTTTTGTTGACATTGTTGAACAGAAATGGCATGCCAACTTAAAAGTTGCCAACTTGTTTGTTCCGCTTTTTGAAAGGATCTTAGAAATTCCAATAATTTGGTCCAGAGGAAGAGCCACTGGTGAGGTTCACTTGTGCATGTCAAGTGGAGAAACATTTCCTAATCTTCATGGACAGCTTGATGTGACAGGGTTGGCTTTTCAAACAATTGGCGCGCCTTCATCATTTTCTGATATTTCAGCAAGCTTATGTTTCCGTGGCCAACGGATATTCTTACACAATGCAAGTGGCTGGTTTGGTGATGTTCCCTTGGAAGCTTCTGGAGATTTTGGCATTCATCCTGAGGAAGGAGAGTTTCATCTTATGTGTCAGGTTTCTTGCGTGGAAGTAAATTCCCTGATGAGAACTTTCAAGATGAAACCTCTCTTGTTTCCGCTGGCTGGTTCAGTGACTGCTGTGTTTAACTGTCAAGGTCCATTGGATGCACCTATATTTGTAGGAAGTGGAATGGTCTCTAGAAGGATATCTCATTCAGTTACTGATTTTCCTCCGTCCTCTGCATCTGAAGCAGTTTTGAAAAGTAAAGAAGCCGGTGCAGTAGCAGCATTTGATCGTGTACCATTTTCATGCGTATCGGCTAATTTCACTTTTAACACTGATAGCTGTGTTGCTGATTTGTATGGAATAAGAGCTAGTCTTGTAGATGGCGGTGAAATTCGAGGGGCAGGGAATGCATGGATTTGCCCAGAGGGTGAAGTTGATGATACATCAATGGATGTGAATTTCTCCGGAAGTTTGTGTTTTGATAAAATTCTGCATCGATATGTACCTGGGTATCTTCAACTAATGCCACTCAAATTAGGGGTTTTAAATGGAGAGACAAAACTTTCTGGTTCGCTTTTGAGACCAAGGTTTGACATTAAATGGACAGCTCCAAAAGCTGAAGGGTCCTTCAGTGATGCTAGAGGAGATATCATAATCTCACACGATTCCATTACTGTTAATTCCTCATCTGCTGCTTTTGATTTGTCATTGAAAGTTCAAACATCTTACACTGACGAAATCTGCTTGCACAGAAAAGATGGTTATGGAAAGAGTGCCATGCCATTTGTTGTTGAGGGAATTGACTTGGATTTGCGTATGCGTAGTTTTGAGTTTTTTAATGTGGTATCGCCTTATACCTTCGATTCTCCGAAACCAATGCATTTAAAAGCAACAGGCAAGATCAAGTTTCAAGGCAAGGTTGTAAAGCCTTGCAGTTTTGAAAATGGACAAGATTTTGGTCTAGATAGAAATAAGCTCCCTGTGACAATGACATATAAAGAAAAGACAGACAGTCTTGTTGGAGAGGTTTCAATTTCTGGTCTTAGATTGAATCAGTTGATGTTGGCGCCTCAGTTGGCTGGATCATTGAGCTTGTCACCTGAGTATATCAAGTTGGATGCCACTGGTAGGCCAGATGAAAGTCTTGTAGTGGAGTTTGTTGGGCCGTTGAAGCCTAATAATGAAGATAATTCCCCAAGTGGACAGTTGTTATCTTTTTTGCTTCAAAAGGGGCAACTAAAAGCAAACATTTGCTTCCAGCCATTTCATTCTGCTAGTTTGGAGATACGTCAGTTACCACTGGATGAGTTGGAGTTGGCCTCACTCCGTGGAACAATACAGAAGGCAGAAATTCAACTTAATTTTCAGAAACGAAGAGGTCATGGACTACTATCTGTACTTCGACCGAAATTCAGTGGAGTGCTAGGTGAAGCCCTTGATGTCGCTGCTCGATGGAGTGGAGATGTTATCACTGTTGAGAAAACGGTCCTGGAGCAAAGCAATAGCCGTTATGAGCTTCAGGGTGAATATGTGTTGCCAGGCTCACGAGACCGCAACCCTGCTGGAAAGGAAAAGGGTGGCTTGTTGGAAAGGGCAATGGCTGGGCATCTGGGAAGTGTCATATCTTCTATGGGAAGGTGGAGAATGAGACTGGAAGTTCCCAGAGCTGAGGTTGCTGAGATGCTTCCCCTAGCCAGACTTGTTTCTCGAAGTACAGATCCCGCTGTTCATTCTAGGTCAAAGGATCTCTTTATTCAGAGTTTGCAGTATGTGGGATTATATACTGAAAGTCTTAAAGAACTACTCGAGGTTATACGGGGACATTATACTCCATCAAATGAAGTTATTCTAGAAGACTTAAATCTTCCAGGTTTAACAGAGCTTAGAGGTAGATGGCATGGCTCTCTTGATGCTAGTGGTGGTGGCAATGGGGATACCATGGCAGAATTTGATTTTCATGGGGAGGATTGGGAGTGGGGTACCTACAAAACTCAGCGAGTTCTGGCAGTTGGTGCATACAGCAATGATGATGGTTTGCGCCTGGAAAAGATGTTTATTCAGAAGGATAATGCTACAATTCATGCAGATGGGACTTTGTTAGGGCCTAAAACGAATCTTCACTTTGCTGTTCTAAATTTTCCTGTTAGTTTAGTTCCGACAGTGATTCAGGTGATTGAATCTTCTGCTACTGATGTGGTTCAATCTTTAAGGAAGTTTTTAGCACCAATTAGGGGTATATTGCACATGGAAGGCGATCTCAGAGGGAATCTTGCAAAACCAGAATGTGATGTGCAAGTAAGGCTCCTGGATGGTGCCATTGGGGGGATCGATCTTGGACGAGCTGAAGTTGTTGCTTCTCTAACTTCAACCAGCCGTTTTCTGTTCAACGCAAAATTTGAGCCAATCATCCAAATTGGGCATGTTCACATTCAAGGAAGTGTTCCTGTTACTTTTGTTCAGAATAACATGTTAGAGGAAGAAGATTTAGAGAAAGATAAAGGTCGAGCAAGTTGGGAAGGCTGGGTGAAAGAAAGAGGTAGGGCATCTGTAGATGATGCCAGTGAAAAAAAACTTTCAAGAGAGCGAAATGAAGAAGGTTGGGATACTCGATTAGCAGAAAGCCTCAAGGGTTTAAACTGGAACCTACTGGATGTAGGGGAAGTCAGAATAGATGCTGATGTTAAAGATGGGGGCATGATGCTGTTAACTGCTTTATCGCCTTATGCTAAATGGCTTCAGGGCAATGCCGACGTCATGCTTCAGGTCAGAGGCACAGTTGAACAACCGGTGCTGGATGGGTATGCATCATTCCACAGGGCATCTATATCATCACCAGTTCTCTGGAAACCACTGTCAAACTTCGGTGGCACAGTTCATGTGAAATCGAATAGGTTGTGCATTACTTCGTTGGAGAGTAGGGTAAGCAGAAGGGGAAAACTATTCGTGAAAGGAAACCTACCCCTTAGAACAAGTGAAGCATCCCTTGGAGATAAAATTGACTTGAAATGTGAAGTTCTGGAAGTGCGGGCAAAGAATATTCTGAGTGCCCAGGTTGACACTCAGATGCAGATAACTGGTTCTATATTGCAACCAAACATCTCTGGAAATATTAAATTGAGCCATGGTGAAGCATATCTACCACATGATAAGGGTAGTGGGGCTACTTCTAATAGATTGGTGTCAAATGAGTCTAGGCTTCTAGCTACTGGTGTCGATCGAGCAGTTGCCTCGAGGTATGTTTCACGGTTTTTCAGTTCCCAACCTGCTGCTTCGAGGACAAAATTCTCTCAACCCTCAGTTGAACCAGCTGAAAAGGAGATGGAGCAAGTGAACATTAAACCGAACATAGATATTCAGCTTAGTGATTTAAAGCTTGCTCTAGGACCAGAATTGAGGGTAGTTTATCCTTTAATTCTGAATTTCGCAGTCAGTGGAGAGCTTGAGTTAAATGGCCCGGCTCATCCCAAATTGATACGACCTAGAGGAGTCTTAACCTTTGAGAATGGTGACGTGAATCTTGTGGCAACTCAGGTGAGGCTCAAGCAAGAGCATCTGAATATTGCGAAATTCGAGCCTGAAATTGGACTAGATCCTATGCTAGATTTAGTTCTTGTAGGTTCGGAGTGGCAATTCAGGATTCAAAGTCGAGCCAGCAATTGGCAGGACAAACTGGTGGTGACATCCACTCGCTCTGTGGAACAGGATGCCCTCTCACCGACCGAGGCTGCTAGAGTCTTTGAAAGTCAACTGGCGGAGTCCATATTGGAAAACGATGGGCAGCTTGCATTCCAAAAGCTTGCCACTACAACACTGGAAAAACTAATGCCAAGAATAGAAGGAAAGGGTGAGTTTGGGCAGGCAAGGTGGAGGCTAGTGTATGCACCGCAGATCCCTAGTTTGCTTTCTGTTGATCCCACGGTGGATCCTCTCAAATCCCTGGCCAGTAATATTTCCTTTGGTACAGAAGTTGAAGTCCAACTCGGGAAACGTCTTCAGGCCTCGATTGTTAGGCAAATGAAAGACTCAGAGATGGCAATGCAATGGACGTTGATCTACCAGCTTACGAGCCGCTTGAGGGTTCTCCTGCAGTCTGCTCCGTCGAAGCGCCTCCTTTTCGAATATTCTGCCACCTCGCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TamB

Protein Analysis

2171

Amino Acids

239.77

Weight (kDa)

6.29

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TamB PF04357 1760 - 2154 1.8e-18 TamB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1751, 5124
AbsI CCTCGAGG 1 cut(s) 5599
Acc36I ACCTGC 1 cut(s) 5641
Acc65I GGTACC 1 cut(s) 4349
AccB1I GGYRCC 3 cut(s) 3436, 4349, 4682
AccB7I CCANNNNNTGG 2 cut(s) 29, 6027
AccI GTMKAC 3 cut(s) 706, 1794, 2039
AccII CGCG 1 cut(s) 2261
AccIII TCCGGA 1 cut(s) 2834
AcoI YGGCCR 3 cut(s) 1960, 2305, 6309
AcyI GRCGYC 3 cut(s) 1748, 3437, 5121
AdeI CACNNNGTG 1 cut(s) 3698
AflIII ACRYGT 2 cut(s) 1936, 4836
AgeI ACCGGT 1 cut(s) 5155
AhdI GACNNNNNGTC 1 cut(s) 3381
AjiI CACGTC 1 cut(s) 5881
AjnI CCWGG 9 cut(s) 1039, 2875, 3858, 3907, 4237, 4410, 4674, 5418, 6306
AjuI GAANNNNNNNTTGG 4 cut(s) 453, 485, 5337, 5369
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 7 cut(s) 291, 297, 2563, 2918, 2944, 3912, 4012
AlwNI CAGNNNCTG 3 cut(s) 1624, 2498, 2585
Ama87I CYCGRG 4 cut(s) 1911, 4175, 5599, 6350
Aor13HI TCCGGA 1 cut(s) 2834
ApaI GGGCCC 1 cut(s) 1763
AscI GGCGCGCC 1 cut(s) 2259
AsiGI ACCGGT 1 cut(s) 5155
Asp700I GAANNNNTTC 3 cut(s) 763, 2202, 4215
Asp718I GGTACC 1 cut(s) 4349
AspLEI GCGC 5 cut(s) 2261, 2263, 3439, 4410, 6477
AsuC2I CCSGG 1 cut(s) 5825
AsuII TTCGAA 1 cut(s) 6486
AvaI CYCGRG 4 cut(s) 1911, 4175, 5599, 6350
AvaII GGWCC 8 cut(s) 559, 1062, 1730, 2148, 2519, 2994, 3856, 5753
AxyI CCTNAGG 1 cut(s) 2388
BaeGI GKGCMC 3 cut(s) 1183, 1763, 5419
BaeI ACNNNNGTAYC 6 cut(s) 2864, 2897, 3647, 3680, 6321, 6354
BalI TGGCCA 3 cut(s) 1962, 2307, 6311
BamHI GGATCC 1 cut(s) 6292
BanI GGYRCC 3 cut(s) 3436, 4349, 4682
BanII GRGCYC 2 cut(s) 258, 1763
BarI GAAGNNNNNNTAC 6 cut(s) 297, 329, 690, 722, 5490, 5522
BauI CACGAG 1 cut(s) 3915
BbsI GAAGAC 2 cut(s) 4230, 6353
Bbv12I GWGCWC 1 cut(s) 332
BbvCI CCTCAGC 1 cut(s) 4038
BceAI ACGGC 3 cut(s) 3520, 3861, 4728
BcgI CGANNNNNNTGC 6 cut(s) 3229, 3263, 5580, 5614, 6476, 6510
BciT130I CCWGG 9 cut(s) 1041, 2877, 3860, 3909, 4239, 4412, 4676, 5420, 6308
BciVI GTATCC 3 cut(s) 678, 4297, 4966
BclI TGATCA 1 cut(s) 739
BcnI CCSGG 1 cut(s) 5825
BcoDI GTCTC 7 cut(s) 291, 297, 2563, 2918, 2944, 3912, 4012
BfmI CTRYAG 5 cut(s) 825, 1625, 2040, 4917, 6455
BfoI RGCGCY 2 cut(s) 3440, 6478
BfuAI ACCTGC 1 cut(s) 5641
BfuI GTATCC 3 cut(s) 678, 4297, 4966
BmcAI AGTACT 1 cut(s) 438
Bme18I GGWCC 8 cut(s) 559, 1062, 1730, 2148, 2519, 2994, 3856, 5753
BmeRI GACNNNNNGTC 1 cut(s) 3381
BmeT110I CYCGRG 4 cut(s) 1911, 4175, 5599, 6350
BmgBI CACGTC 1 cut(s) 5881
BpiI GAAGAC 2 cut(s) 4230, 6353
BplI GAGNNNNNCTC 2 cut(s) 3674, 3706
BpmI CTGGAG 4 cut(s) 979, 1260, 2388, 3881
Bpu10I CCTNAGC 2 cut(s) 1673, 4038
Bpu14I TTCGAA 1 cut(s) 6486
BpuEI CTTGAG 5 cut(s) 141, 4979, 5831, 5894, 6463
BpuMI CCSGG 1 cut(s) 5825
Bsa29I ATCGAT 2 cut(s) 2866, 4698
BsaAI YACGTR 1 cut(s) 1972
BsaHI GRCGYC 3 cut(s) 1748, 3437, 5121
BsaI GGTCTC 4 cut(s) 291, 2563, 2944, 3912
BsaWI WCCGGW 3 cut(s) 1133, 2834, 5155
BsaXI ACNNNNNCTCC 4 cut(s) 1007, 1037, 1560, 1590
Bse118I RCCGGY 2 cut(s) 2636, 5155
Bse21I CCTNAGG 1 cut(s) 2388
Bse3DI GCAATG 9 cut(s) 248, 883, 967, 3972, 4303, 4399, 5119, 6411, 6416
BseAI TCCGGA 1 cut(s) 2834
BseBI CCWGG 9 cut(s) 1041, 2877, 3860, 3909, 4239, 4412, 4676, 5420, 6308
BseCI ATCGAT 2 cut(s) 2866, 4698
BseMI GCAATG 9 cut(s) 248, 883, 967, 3972, 4303, 4399, 5119, 6411, 6416
BsePI GCGCGC 1 cut(s) 2259
BseRI GAGGAG 4 cut(s) 5, 285, 3027, 5868
BseSI GKGCMC 3 cut(s) 1183, 1763, 5419
BseYI CCCAGC 3 cut(s) 789, 3971, 4890
BsgI GTGCAG 2 cut(s) 1191, 2661
Bsh1236I CGCG 1 cut(s) 2261
Bsh1285I CGRYCG 3 cut(s) 3776, 5587, 6090
BshNI GGYRCC 3 cut(s) 3436, 4349, 4682
BshTI ACCGGT 1 cut(s) 5155
BshVI ATCGAT 2 cut(s) 2866, 4698
BsiEI CGRYCG 3 cut(s) 3776, 5587, 6090
BsiHKAI GWGCWC 1 cut(s) 332
BsiHKCI CYCGRG 4 cut(s) 1911, 4175, 5599, 6350
BsiSI CCGG 5 cut(s) 1134, 2637, 2835, 5156, 5825
BsiWI CGTACG 1 cut(s) 98
BslFI GGGAC 4 cut(s) 101, 545, 4203, 4474
BsmAI GTCTC 7 cut(s) 291, 297, 2563, 2918, 2944, 3912, 4012
BsmFI GGGAC 4 cut(s) 101, 545, 4203, 4474
BsmI GAATGC 3 cut(s) 2379, 2782, 6155
Bso31I GGTCTC 4 cut(s) 291, 2563, 2944, 3912
BsoBI CYCGRG 4 cut(s) 1911, 4175, 5599, 6350
Bsp119I TTCGAA 1 cut(s) 6486
Bsp120I GGGCCC 1 cut(s) 1759
Bsp1286I GDGCHC 5 cut(s) 258, 332, 1183, 1763, 5419
Bsp13I TCCGGA 1 cut(s) 2834
Bsp19I CCATGG 2 cut(s) 4308, 5491
BspDI ATCGAT 2 cut(s) 2866, 4698
BspEI TCCGGA 1 cut(s) 2834
BspFNI CGCG 1 cut(s) 2261
BspMAI CTGCAG 3 cut(s) 829, 1629, 6459
BspMI ACCTGC 1 cut(s) 5641
BspQI GCTCTTC 1 cut(s) 2152
BspT104I TTCGAA 1 cut(s) 6486
BspT107I GGYRCC 3 cut(s) 3436, 4349, 4682
BspTNI GGTCTC 4 cut(s) 291, 2563, 2944, 3912
BsrDI GCAATG 9 cut(s) 248, 883, 967, 3972, 4303, 4399, 5119, 6411, 6416
BsrFI RCCGGY 2 cut(s) 2636, 5155
BssAI RCCGGY 2 cut(s) 2636, 5155
BssHII GCGCGC 1 cut(s) 2259
BssNI GRCGYC 3 cut(s) 1748, 3437, 5121
BssSI CACGAG 1 cut(s) 3915
BssT1I CCWWGG 6 cut(s) 393, 2355, 2954, 4308, 5353, 5491
Bst2BI CACGAG 1 cut(s) 3915
Bst2UI CCWGG 9 cut(s) 1041, 2877, 3860, 3909, 4239, 4412, 4676, 5420, 6308
Bst6I CTCTTC 3 cut(s) 300, 2152, 3739
BstACI GRCGYC 3 cut(s) 1748, 3437, 5121
BstAPI GCANNNNNTGC 2 cut(s) 2777, 4382
BstBAI YACGTR 1 cut(s) 1972
BstBI TTCGAA 1 cut(s) 6486
BstDSI CCRYGG 5 cut(s) 2302, 3697, 4308, 5491, 6285
BstENI CCTNNNNNAGG 2 cut(s) 1044, 2391
BstFNI CGCG 1 cut(s) 2261
BstH2I RGCGCY 2 cut(s) 3440, 6478
BstHHI GCGC 5 cut(s) 2261, 2263, 3439, 4410, 6477
BstMAI GTCTC 7 cut(s) 291, 297, 2563, 2918, 2944, 3912, 4012
BstMCI CGRYCG 3 cut(s) 3776, 5587, 6090
BstNI CCWGG 9 cut(s) 1041, 2877, 3860, 3909, 4239, 4412, 4676, 5420, 6308
BstNSI RCATGY 6 cut(s) 1007, 1940, 2081, 2188, 4793, 4840
BstSFI CTRYAG 5 cut(s) 825, 1625, 2040, 4917, 6455
BstSLI GKGCMC 3 cut(s) 1183, 1763, 5419
BstUI CGCG 1 cut(s) 2261
BstV2I GAAGAC 2 cut(s) 4230, 6353
BstX2I RGATCY 7 cut(s) 1198, 2124, 4087, 4114, 5959, 6258, 6292
BstXI CCANNNNNNTGG 2 cut(s) 2145, 5211
BstYI RGATCY 7 cut(s) 1198, 2124, 4087, 4114, 5959, 6258, 6292
Bsu15I ATCGAT 2 cut(s) 2866, 4698
Bsu36I CCTNAGG 1 cut(s) 2388
BsuI GTATCC 3 cut(s) 678, 4297, 4966
BsuTUI ATCGAT 2 cut(s) 2866, 4698
BtgI CCRYGG 5 cut(s) 2302, 3697, 4308, 5491, 6285
BtrI CACGTC 1 cut(s) 5881
BveI ACCTGC 1 cut(s) 5641
CaiI CAGNNNCTG 3 cut(s) 1624, 2498, 2585
CfoI GCGC 5 cut(s) 2261, 2263, 3439, 4410, 6477
Cfr10I RCCGGY 2 cut(s) 2636, 5155
ClaI ATCGAT 2 cut(s) 2866, 4698
CspAI ACCGGT 1 cut(s) 5155
CspCI CAANNNNNGTGG 2 cut(s) 2314, 2349
DinI GGCGCC 1 cut(s) 3438
DraI TTTAAA 7 cut(s) 54, 1099, 1422, 2916, 3255, 5004, 5739
DraIII CACNNNGTG 1 cut(s) 3698
DriI GACNNNNNGTC 1 cut(s) 3381
EaeI YGGCCR 3 cut(s) 1960, 2305, 6309
Eam1104I CTCTTC 3 cut(s) 300, 2152, 3739
Eam1105I GACNNNNNGTC 1 cut(s) 3381
EarI CTCTTC 3 cut(s) 300, 2152, 3739
EciI GGCGGA 1 cut(s) 6137
Eco130I CCWWGG 6 cut(s) 393, 2355, 2954, 4308, 5353, 5491
Eco147I AGGCCT 1 cut(s) 6368
Eco24I GRGCYC 2 cut(s) 258, 1763
Eco31I GGTCTC 4 cut(s) 291, 2563, 2944, 3912
Eco32I GATATC 4 cut(s) 1123, 1599, 2571, 3019
Eco47I GGWCC 8 cut(s) 559, 1062, 1730, 2148, 2519, 2994, 3856, 5753
Eco81I CCTNAGG 1 cut(s) 2388
Eco88I CYCGRG 4 cut(s) 1911, 4175, 5599, 6350
EcoNI CCTNNNNNAGG 2 cut(s) 1044, 2391
EcoO109I RGGNCCY 5 cut(s) 559, 1079, 1760, 2994, 4472
EcoRI GAATTC 1 cut(s) 1155
EcoRII CCWGG 9 cut(s) 1039, 2875, 3858, 3907, 4237, 4410, 4674, 5418, 6306
EcoRV GATATC 4 cut(s) 1123, 1599, 2571, 3019
EcoT14I CCWWGG 6 cut(s) 393, 2355, 2954, 4308, 5353, 5491
EcoT22I ATGCAT 4 cut(s) 941, 2782, 3252, 5176
EcoT38I GRGCYC 2 cut(s) 258, 1763
EgeI GGCGCC 1 cut(s) 3438
EheI GGCGCC 1 cut(s) 3438
ErhI CCWWGG 6 cut(s) 393, 2355, 2954, 4308, 5353, 5491
FalI AAGNNNNNCTT 8 cut(s) 399, 431, 3935, 3967, 4103, 4135, 5337, 5369
FaqI GGGAC 4 cut(s) 101, 545, 4203, 4474
FauI CCCGC 2 cut(s) 4099, 5388
FauNDI CATATG 1 cut(s) 1894
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 3 cut(s) 706, 1794, 2039
FriOI GRGCYC 2 cut(s) 258, 1763
GlaI GCGC 5 cut(s) 2260, 2262, 3438, 4409, 6476
GsaI CCCAGC 3 cut(s) 793, 3975, 4894
GsuI CTGGAG 4 cut(s) 979, 1260, 2388, 3881
HaeII RGCGCY 2 cut(s) 3440, 6478
HapII CCGG 5 cut(s) 1134, 2637, 2835, 5156, 5825
HhaI GCGC 5 cut(s) 2261, 2263, 3439, 4410, 6477
Hin1I GRCGYC 3 cut(s) 1748, 3437, 5121
Hin6I GCGC 5 cut(s) 2259, 2261, 3437, 4408, 6475
HinP1I GCGC 5 cut(s) 2259, 2261, 3437, 4408, 6475
HincII GTYRAC 6 cut(s) 1411, 1795, 2056, 5076, 5425, 6116
HindII GTYRAC 6 cut(s) 1411, 1795, 2056, 5076, 5425, 6116
HindIII AAGCTT 5 cut(s) 413, 2290, 2361, 5741, 6164
HpaI GTTAAC 1 cut(s) 5076
HpaII CCGG 5 cut(s) 1134, 2637, 2835, 5156, 5825
Hpy99I CGWCG 4 cut(s) 646, 1799, 5123, 6472
HpyCH4IV ACGT 7 cut(s) 1748, 1971, 3658, 5121, 5880, 6358, 6416
HpySE526I ACGT 7 cut(s) 1748, 1971, 3658, 5121, 5880, 6358, 6416
Hsp92I GRCGYC 3 cut(s) 1748, 3437, 5121
HspAI GCGC 5 cut(s) 2259, 2261, 3437, 4408, 6475
KasI GGCGCC 1 cut(s) 3436
Kpn2I TCCGGA 1 cut(s) 2834
KpnI GGTACC 1 cut(s) 4353
Ksp22I TGATCA 1 cut(s) 739
KspAI GTTAAC 1 cut(s) 5076
LguI GCTCTTC 1 cut(s) 2152
LmnI GCTCC 7 cut(s) 1526, 2983, 3351, 3862, 4677, 5692, 6469
MaeII ACGT 7 cut(s) 1748, 1971, 3658, 5121, 5880, 6358, 6416
MfeI CAATTG 3 cut(s) 522, 2253, 6025
MflI RGATCY 7 cut(s) 1198, 2124, 4087, 4114, 5959, 6258, 6292
MhlI GDGCHC 5 cut(s) 258, 332, 1183, 1763, 5419
MlsI TGGCCA 3 cut(s) 1962, 2307, 6311
MluNI TGGCCA 3 cut(s) 1962, 2307, 6311
Mly113I GGCGCC 1 cut(s) 3437
MmeI TCCRAC 9 cut(s) 282, 665, 3370, 3465, 3660, 3936, 4550, 5259, 6370
Mox20I TGGCCA 3 cut(s) 1962, 2307, 6311
Mph1103I ATGCAT 4 cut(s) 941, 2782, 3252, 5176
MroI TCCGGA 1 cut(s) 2834
MroXI GAANNNNTTC 3 cut(s) 763, 2202, 4215
MscI TGGCCA 3 cut(s) 1962, 2307, 6311
MslI CAYNNNNRTG 5 cut(s) 934, 2671, 2868, 4455, 5432
Msp20I TGGCCA 3 cut(s) 1962, 2307, 6311
MspA1I CMGCKG 3 cut(s) 2482, 4094, 5678
MspI CCGG 5 cut(s) 1134, 2637, 2835, 5156, 5825
MssI GTTTAAAC 1 cut(s) 5004
MunI CAATTG 3 cut(s) 522, 2253, 6025
Mva1269I GAATGC 3 cut(s) 2379, 2782, 6155
MvaI CCWGG 9 cut(s) 1041, 2877, 3860, 3909, 4239, 4412, 4676, 5420, 6308
MvnI CGCG 1 cut(s) 2261
NarI GGCGCC 1 cut(s) 3437
NciI CCSGG 1 cut(s) 5825
NcoI CCATGG 2 cut(s) 4308, 5491
NdeI CATATG 1 cut(s) 1894
NmuCI GTSAC 6 cut(s) 2230, 2494, 3352, 3465, 5876, 6046
NsiI ATGCAT 4 cut(s) 941, 2782, 3252, 5176
NspI RCATGY 6 cut(s) 1007, 1940, 2081, 2188, 4793, 4840
NspV TTCGAA 1 cut(s) 6486
PaeI GCATGC 2 cut(s) 1007, 2081
PaeR7I CTCGAG 3 cut(s) 1911, 4175, 5599
PalAI GGCGCGCC 1 cut(s) 2259
PauI GCGCGC 1 cut(s) 2259
PceI AGGCCT 1 cut(s) 6368
PciI ACATGT 2 cut(s) 1936, 4836
PciSI GCTCTTC 1 cut(s) 2152
PctI GAATGC 3 cut(s) 2379, 2782, 6155
PdmI GAANNNNTTC 3 cut(s) 763, 2202, 4215
Pfl23II CGTACG 1 cut(s) 98
PflFI GACNNNGTC 1 cut(s) 1799
PflMI CCANNNNNTGG 2 cut(s) 29, 6027
PfoI TCCNGGA 2 cut(s) 3858, 4674
PinAI ACCGGT 1 cut(s) 5155
Ple19I CGATCG 1 cut(s) 5587
PluTI GGCGCC 1 cut(s) 3440
PmeI GTTTAAAC 1 cut(s) 5004
Ppu21I YACGTR 1 cut(s) 1972
PpuMI RGGWCCY 2 cut(s) 559, 2994
PscI ACATGT 2 cut(s) 1936, 4836
Psp5II RGGWCCY 2 cut(s) 559, 2994
Psp6I CCWGG 9 cut(s) 1039, 2875, 3858, 3907, 4237, 4410, 4674, 5418, 6306
PspFI CCCAGC 3 cut(s) 789, 3971, 4890
PspGI CCWGG 9 cut(s) 1039, 2875, 3858, 3907, 4237, 4410, 4674, 5418, 6306
PspLI CGTACG 1 cut(s) 98
PspOMI GGGCCC 1 cut(s) 1759
PspPPI RGGWCCY 2 cut(s) 559, 2994
PspXI VCTCGAGB 2 cut(s) 4175, 5599
PstI CTGCAG 3 cut(s) 829, 1629, 6459
PstNI CAGNNNCTG 3 cut(s) 1624, 2498, 2585
PsuI RGATCY 7 cut(s) 1198, 2124, 4087, 4114, 5959, 6258, 6292
PsyI GACNNNGTC 1 cut(s) 1799
PteI GCGCGC 1 cut(s) 2259
PvuI CGATCG 1 cut(s) 5587
PvuII CAGCTG 1 cut(s) 5678
RseI CAYNNNNRTG 5 cut(s) 934, 2671, 2868, 4455, 5432
SalI GTCGAC 1 cut(s) 1793
SapI GCTCTTC 1 cut(s) 2152
ScaI AGTACT 1 cut(s) 438
SduI GDGCHC 5 cut(s) 258, 332, 1183, 1763, 5419
SfcI CTRYAG 5 cut(s) 825, 1625, 2040, 4917, 6455
SfoI GGCGCC 1 cut(s) 3438
Sfr274I CTCGAG 3 cut(s) 1911, 4175, 5599
SfuI TTCGAA 1 cut(s) 6486
SgsI GGCGCGCC 1 cut(s) 2259
SinI GGWCC 8 cut(s) 559, 1062, 1730, 2148, 2519, 2994, 3856, 5753
SlaI CTCGAG 3 cut(s) 1911, 4175, 5599
SmiI ATTTAAAT 1 cut(s) 1422
SmiMI CAYNNNNRTG 5 cut(s) 934, 2671, 2868, 4455, 5432
SmlI CTYRAG 8 cut(s) 156, 1911, 4175, 4994, 5599, 5810, 5909, 6442
SmoI CTYRAG 8 cut(s) 156, 1911, 4175, 4994, 5599, 5810, 5909, 6442
SphI GCATGC 2 cut(s) 1007, 2081
SseBI AGGCCT 1 cut(s) 6368
SspDI GGCGCC 1 cut(s) 3436
SspI AATATT 5 cut(s) 5407, 5479, 5929, 6319, 6491
StuI AGGCCT 1 cut(s) 6368
StyI CCWWGG 6 cut(s) 393, 2355, 2954, 4308, 5353, 5491
SwaI ATTTAAAT 1 cut(s) 1422
TaiI ACGT 7 cut(s) 1751, 1974, 3661, 5124, 5883, 6361, 6419
TaqII GACCGA 2 cut(s) 3790, 6104
TatI WGTACW 4 cut(s) 334, 436, 3766, 4082
TauI GCSGC 4 cut(s) 782, 815, 2031, 6441
TseFI GTSAC 6 cut(s) 2230, 2494, 3352, 3465, 5876, 6046
Tsp45I GTSAC 6 cut(s) 2230, 2494, 3352, 3465, 5876, 6046
TspGWI ACGGA 6 cut(s) 1383, 2291, 2326, 2586, 3686, 6456
Tth111I GACNNNGTC 1 cut(s) 1799
Van91I CCANNNNNTGG 2 cut(s) 29, 6027
VpaK11BI GGWCC 8 cut(s) 559, 1062, 1730, 2148, 2519, 2994, 3856, 5753
XagI CCTNNNNNAGG 2 cut(s) 1044, 2391
XbaI TCTAGA 4 cut(s) 767, 2561, 3331, 4219
XceI RCATGY 6 cut(s) 1007, 1940, 2081, 2188, 4793, 4840
XcmI CCANNNNNNNNNTGG 2 cut(s) 6061, 6178
XhoI CTCGAG 3 cut(s) 1911, 4175, 5599
XmiI GTMKAC 3 cut(s) 706, 1794, 2039
XmnI GAANNNNTTC 3 cut(s) 763, 2202, 4215
ZraI GACGTC 2 cut(s) 1749, 5122
ZrmI AGTACT 1 cut(s) 438
Zsp2I ATGCAT 4 cut(s) 941, 2782, 3252, 5176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.