AT2G25730

phosphatidylinositol-3-phosphate binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005768
Physical Location & Seq
Reverse (-)
10956263 .. 10972951
16689 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25730.2

Sequence Viewer

Length: 7395 bp
ATGGATAAAGAGACGGAGATTCTCTCCCGTCTCGCGGCGAACCACCTTCATCTGGCTCAATTCGAGCCATTGAAGGCTACGTTACTCGCTCTCAGGGTTCGTAACCCTGACCTCGCACTCACCATTCTCCAAACCATCGTCTCCAACGCTGGAAGATTCGATAATGTCCTCTGGTCACGCTCTTGTCCTTCCCCGTCTCTTCTCTCGTTCCTCTCCACGATTGAGCTTCTGAGATTCGAAAATCCTACTTCTCCTTGGGGATTTGATTCAGAAACTCTAAGTTTGCGTGCCGATTTCTTGTTGATGGTTCAGGTTTTGATCGATAGAGTTACAGAGAGGATTAAGGAAGATGAGGAGAGTGAGGATGAAAATTCTGGATTAGGGAATTGTTTAAGGGTGTTGCAAGGTGTTTTGGAGTTAGGTGTTGAGAGGTTGAAGTTTGTTGTTGATACTAGTAGTAGTGAAGGAAGTAATAAGATTGAGGAAGATGCAGTTGTGTCTTTGAGGAGTATAGTATTGGATTACTCTGATGTTTTCGATGCTTTATGTTGTAATATTCAGAGGCAACTTGCGGGTTGCGAGAGTTACGGTACATGTTTGGTTGAGGAAGTTCAGGGAGAAGAACAGAGAAAGGAGATGAATGAGGCCACATGTATTGGTTCTCCGGAGCTGGATAACATCAATGTGTTTGCTTTGATACAGAGGAATGTTCAGTTAGCACAGTTGGATGCTATGAAAACAAAGTTGGATGAAGGTGATGAGCGCGGGGCAGCTGATCGCATTCGTTATCTTCACCTTGATTATGGAGTAGAGAAAGAGAACTATCATGCTGTTCTAAAAGCTCTCCTTTCAAGAGTTATGGAGAAAAAGGATGAATATGGTGATTCCTGGCACATGGTGCGCCAGAACTTGCTGTTTATGTATAAAGAAGCTCTCTCATCGAATTGTGGAGATCTTGTTCAGATGATCCAGGGTATTCAAGATGATATGCTCCTCCCACATAGCCAACTACATTTATCTCTCGACAATGAACAAATTCCACTCCCTCTTGAATGTTTCCGGCGATATCTTGTAGACTTGAAAACTGAGAGAAATATAGAGGACAAAAGTTCTCCTATGAGCAGGGCAATTAATTCTTGTCTCAGAGATATGTATCATTATGCTCGTATTTCTGGATCACATGTTCTTGAGTGTGTGATGTGTGCTGCTTTGTCTTCTGTAAAGAAAGAGAAGCTTCAGGAGGCTAATGATGTTCTTACTTTGTTTCCCCGACTTCGCCCTTTAGTAGCCTCCATGGGTTGGGATCTATTGCCGGGCAAAACTGCAACCCGTAGAAAATTGATGCGGCTACTTTGGACTAGTGACTCGCAAGCACTTCGGCTAGAAGAATCTTCTCTTTATGGAAACCAGACAGATGAACTGGAACTTGCATCTTTCGCTGCTTGTGTCAATTCTGGTAAATCATGGACTCCAAAGGCATCTTTCTTGATGCATGGTAATGTGTCATCCGCGCATGATGATGCGGAGGTGGATCCTTTTGTTGAAAATCTTGTATTGGAAAGGCTTTCAGCGCAAAGTCCACTTCGGGTATTGTTTGACGTTGTTCCGGGCATAAAATTCCAAGATGCTATTTCACTGATTAGTATGCAACCTATTGCTTCAACTGCAGAAGCCTGGAAGAGGATAGAAGATATTGAACTGATGCATATGCGTTATGCTCTGGAGGCAATCGTTTTAGCACTAGGTGCAATGGAAGAGGCTATGAAGGATGAGACAGATGCTAGTCATCGAGTAGTATTTTACCATTTAAAAGACCTCACTAACCATTTGGAGGCCATTAAAAATGTTCCACGCAAGATAATGATGGTGAACATAGTTATTTCACTCTTACATATTGATGATATCCGTCTCAGTTCTACGCAAAGTGCCTCCTCGGCATGTTTTTCTGAAAAAAGTAACACACCTGGTTTGGATCCTGGCGATCTTGGTACAGAAGGGGAAAAGGAAATTGTTATTTCTTTCACAAAACAGCTACTCGATGTTTTACGCCGCAATCTTCCATCACATCCAATTGAACAAGAGTGTCAGCTGGATGGTAATTACAGTACTGATGGAAGACAGGCTTTAGAATGGAGAGTATCCATGGCTAAGCGTTTCATTGAAGATTGTGAATGGCGATTATCTGTTATGCAGCATCTTCTGCCACTTTCTGAACGCCAGTGGGGTTTAAAGGAGGTTTTGAGTATTCTAAGGGCAGCCCCTGAAAAACTGCTTAATCTCTGTATGCAAAGAGCTAAGTATGACATTGGAGAAGAGGCAGTTAATCGGTTTGCGTTATCAGCAGAGGACAAAGCTACTCTTGAATTAGCTGAATGGGTTGATAATGCGTTCAAAGGAACACTGGTAGAAGATGTAATGTCTCGTACTGCTGAAGGAGCAGCTGCCGTGCAAGATTTAGATTTTCATTCTTTAGGTTCTCAATTGAGTCCATTGGCTATGGTTTTACTTTTTGCGCAGTCTCAAGTTATGTTATCGGAAATTTACCCTGGAGGAGCTCCGAAGGTGGGGTTTACTTACTGGGATCAGGTCCACGAAGTTGCAATAATTTCTGTATTGCGAAGGATCTTAAAGCGTCTGCAGGAATTCCTTGAACAGGATGACCCTCAAATTCTTCAAGCCAGTTTTAGTGGAGATACCATAATTTCATCTTGCACGGAATCTCATAGACAGGGACAAAAAGATCGTGCTCTTGCAATGCTACATCAAATGATTGAGGATGCTCATAGGGGCAAGCGTCAGTTCCTGAGTGGTAAGCTTCATAACTTAGCGAGAGCACTCGCTGATGAAAAACCAGAAGTTGACGTACTCAAAGGGGACGGATCAGACATGGCCGTTGAGAAGGATGGAGTTCTTGGTCTTGGGCTAAAATATACAAAGCAAAGTCCTGGTTCAGCAAATAGAGCCGTGGATGGAAATCCTGTTTCACATGAAACAGAAGACAAGGGAAAGAAGTCATTTGGCCCATTAAGCAACAAAACCTCTACTTATCTATCTCAGTTTATCCTCTATACTGCTGCTATTGGTGATATAGTAGATGGAACTGACACAACCCATGATTTCAACTTTTTCTCTCTTGTTTATGAATGGCCTAAAGACCTATTGACGCGTCTGGTTTTTGATCGAAGTAGCACAGATGCAGCTGCAAAAGTTGCTGAGGTTATGTCTGCTGATTTTGTTCATGAAGTGATATCAGCATGTGTTCCCCCAGTTTATCCCCCACGTTCTGGTCATGGGTGGGCTTGTATTCCCGTCATTCCAACCACTCCATGTTCCCACTCAGAGGGTAAAGTGCTCTCTCCTTCAATAGAGGCTAAACCCAACTGTTATGTCCGTTCCTCAGCAACACCTGGTGTCCCTCTGTATCCTCTTCAGTTGGATGTTATCAGGCATTTGGTAAAAATTTCACCAGTACGAGCAGTTTTAGCTTGCGTCTTTGGTGGGAGCATATTGTACAATGGCAGTGATTCTATCATATCTAGCTCCTTGAACGATGAGTTTCCAAGTTCTCCTGATGCAGACAGATTGTTTTATGAATTTTCTCTTGATCAGTCTGAGAGGTATCCCACTTTAAACCGATGGATACAGATGCAGACTAATCTGCATCGAGTTTCGGAATTTGTTGTGACACCGAAGCAAAAACCTGATGACACACGGATTAAGCCTGATGAAAGAACTGGGATCAAGAGACTTCTTGAACATGATAGTGACTCAGAGTCAGATACAGAAGAAACATTTTCTAAAAATAACATTCAACCAGCATTGACAGACGGCAGTGCTCGTGATGGTGGATCCTTTGAAAATGGAGTTTGTAGAACTGATCCTACCGTTTTCCTTTCTTTTGATTGGGAGAATGAAGTACCGTATGAGAAAGCTGTAAATAGACTAATTGATGAAGGAAAACTAATGGATGCTTTAGCACTTTCGGACCGCTTCTTGCGGAATGGGGCTTCTGATTGGTTACTTCAGCTCCTAATCAAAAGTAGAGAAGAAAATCCTTCAACATCAGGACGATCTCAGGGATATGGAGGCCAGAGCAACAGTTGGCAGTATTGCCTGCGGCTAAAGGACAAACAGCTGGCAGCAACACTGGCTCTTAAGTGTTGCATAGGAGACAAGCTCTGCAGAAGTACAGCCACATACTTTCGGCAGATGATCGCCATAATAGCTGGCAAGAGGTTATCTTTCTTCCTTTTGTTTGAGATCATGTTTGGCTCTTGGTATGCTCGATGTGTCACTCTCAAAAATCTAAATGGGAAACAGGTAGAAGCCGAATGTAAAGAAGACCCTGAAGGCTTGGCTCTAAGATTGGCTGGGAAAGGAGCTGTTTCCGCTGCGCTAGAAGTGGCTGAAAGTGCAGGATTATCAATAGATCTTAGAAGAGAATTACAAGGACGGCAGCTTGTAAAGCTTCTAACCACTGATCCACTCAATGGTGGTGGTCCGGCGGAAGCATCTCGGTTTTTATCTTCACTTCAAGACTCGGCTGATGCTCTACCAGTGGTTATGGGTGCAATGCAATTATTACCTGACCTTCGGTCGAAACAGCTCCTGGTCCATTTCTTTCTCAAGCGAAGAGATAGCAATCTGTCGGATTTGGAAGTCGCCCGGCTTAATTCTTGGGCTTTAGGTCTGAAAGTGTTAGCTGCATTACCACTTCCTTGGCAACAGAGATGCTCTTCGCTTCATGAGCATCCCAATTTAATATTTGAAGCTCTGCTAATGAGAAAACAATTACAATACGCCTCACTGATACTCAAAGAGTTCCCAGCATTAAGAGATAACAATGTTATCATGGCCTATGCAGCAAAAGCTATTTCTGTGACAATTATCCCACCACCAAGAGAACCTCGAATAACTGTGTCTGCGTCAAGGTTAAGACAGAAATCAAGAGCAGGGCCAGCAGTAAAAGCATCCTTCACTAGTAGCTTAAGCAATTTTCAGAGAGAGGCTCGAAGGGCATTCTCATGGGCTCCACGTAATGCTGAAAACCGGACGACGTCAAAGGATGTTTATCGCAAAAGAAAGAATTCCGGACTGGGGGCTTCTGAGAGAGCTGCATGGGAGGCGATGACAGGTATTCAAGAGGACCAGGGATCATCTTATTCGGCAGATGGGCAAGATAGGCTACCTTCTGTTTCTATTGCTGAAGAATGGATGCTAACTGGCGACAAAACCAAAGATGAAGGTGTTCGTGCATCTCACAAGTATGAAAGCACCCCCGATATTATTCTCTTTAAGGCTCTACTATCACTTTGTTCAGATGAGCTAGTATCAGCAAGAAGTGCCATGGACCTATGCATCAGTCAAATGAAAAACGTATTGAGCTCCAAACAGTTGTCAGAGGGTGCGTCTGTTGAAACAATTGGCCGAGCATATCATGCAACGGAGGCATTTGTGCAGGGTTTGTCGTATGCAAAATCATTGCTGAGAAAGCTTCTAGGTACCACTGAATCGACCAATAACAATGGTGAAAGAAGTAGAGATGTTGATGATATATCTTCTGATGCTGGCAGCTCCAGTGTTGGCAGTCAGTCAACGGATGAACCATCGGATGTTCTCTCACTTACAGAAATCTGGTTGGGGCGCGCAGAGTTGCTGCAGAGCCTCTTAGGGTCTGGAATTTCTACTTCTCTTGATGACATTGCTGATCAATTGTCATCCGAATGTCTACGAGACAGATTAATATCTGATGAACGATATAGTATGGCGGTGTATATGTGCAAGAAATGCAAGATTGATGTTTTCCCCGTGTGGAAAGCATGGGGTCTTGCTTTGTTACGGATGGAGCGCTATGCTCAAGCTCGAGTCAAATTCAAGCAAGCCTTTCAATTAAAGGGGGAAGACATTCCTGATGTCATTCAGGAGATAATAAATACAATAGAAGGAGGTCCGCCTGTGGATGTGTCAATTGTACGTTCCATGTATGACCATTTGGCGAAAAGCGCACCTACAATTTTGGACGATTCTTTATCAGCAGACTCATACCTAAATGTTCTGCATATGCCATCCACTTTCCCTCGTTCAGAGAGGTCGCGGAGATCTCTGGAATCGGAAAAGAATAGTTCTGTACCTGGTTCAGACTTTGAAGATGGGCCCCGAAGCAACTTGGATACTACACGCTATTCTGAGTGCACCAACTACTTGCAGGAACATGCTCGCCAAAACCTGCTTGGGTTTATGTTCCGCCATGGTCACTTTAAAGATGCATGCATGTTATTCTTTCCGCAAAGTGGTCTTCCCCCTCCTTTGCAAACTTCATCTGTGGGCGCAGTAAGCACATCTTCATCACCTCAACGAACTGATCCCTTGGCAACTGAATATGGGACCATTGAAAGTTTGTGCGAGTTCTGTGTTGGTTATGGAGCTATCTCATCCCTTGAGGAAGTAATTACAGAAAGACTTGAATCCGCAAAGAATCAAGATCAAGCCATAAATCAGTACATAGCTGGAGCTCTTACTCGTATCTGTGCTTTCTTTGAGATCAACCGGCATTTCAATTACCTATACAAGTTTCTGGTACTCAAGAAGGATTATGTCACCTCTGGGTATTGTTGTATTCAGCTTTTTATGAATTCTACAACTCAGGAGGATGCTGTAAGGCATCTTGAGCATGCAAAGAAATACTGGTCTCTAACTATCCTCGGGGTACAGGCACACTTTGAAGAAGCATTGACAGCGCGTCATAGAGGTTCAGACTCAAAAAAACTTGTTACAAAGGGTGTTAGAGGAAAAAGTGCCGCAGAGAAGCTGAGTGAAGAAACTCTTGTTAAGTTGTCCTCACGGGTGAAAATGCAGATTGATGTGGTGAAGTCCTTCAGTGACTCTGAAGGAGCACCATGGAAGCATTCCTTGTTTGGAAATCCAAATGATTCAGAGACATCCAGGAGAAGATGTGAAATAGTGGAGACTCTTGTTGAGAAAAATTTCGACTTAGCTTATTCTGTTATATATGAATTCAAGCTCTCAGCTGTTGATATATATGCTGGTGTTGCTACGTCACTAGCTGATAGGAAGAAAGGCAGTCAGTTGACAGAACTTTTCAAAAACATTAAGGGAACAATCCAGGATGATGACTGGGATCAGGTCCTGGGTGCTGCCATCAATATATATGCCAACAAGCACAAAGAGCGCCCTGACCGTCTCATCGACATGTTAACAAGCAGCCATCGAAAGGTGCTGGCTTGTGTGGTATGTGGCCGCCTGAAAAGCGCATTCCAGATTGCATCTAAAAGCGGAAGCGTGGCTGATGTTCAATATGTAGCTCATCAAGCCTTACATGCCAATTCGCACACAGTACTCGATATGTGCAAGCAATGGCTAGCTAAATACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2464

Amino Acids

274.77

Weight (kDa)

5.65

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ZFYVE26 PF25569 2366 - 2484 1.6e-33 ZFYVE26-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 5060
Acc16I TGCGCA 1 cut(s) 2514
Acc36I ACCTGC 1 cut(s) 6234
Acc65I GGTACC 1 cut(s) 5501
AccB1I GGYRCC 1 cut(s) 5501
AccB7I CCANNNNNTGG 3 cut(s) 1299, 3284, 4490
AccI GTMKAC 2 cut(s) 1074, 5728
AccII CGCG 7 cut(s) 35, 765, 1511, 3166, 5646, 6094, 6739
AccIII TCCGGA 2 cut(s) 664, 5090
AcoI YGGCCR 3 cut(s) 2889, 5425, 7255
AcuI CTGAAG 8 cut(s) 1220, 2451, 3413, 4007, 4368, 5226, 6859, 6906
AcyI GRCGYC 1 cut(s) 5057
AdeI CACNNNGTG 3 cut(s) 898, 1745, 3410
AfeI AGCGCT 1 cut(s) 5849
AflII CTTAAG 2 cut(s) 4154, 4987
AflIII ACRYGT 6 cut(s) 593, 650, 1180, 3164, 7209, 7389
AhdI GACNNNNNGTC 3 cut(s) 3772, 4594, 6738
AhlI ACTAGT 3 cut(s) 452, 1358, 4979
AjuI GAANNNNNNNTTGG 2 cut(s) 728, 760
AloI GAACNNNNNNTCC 4 cut(s) 942, 974, 1167, 1199
Alw21I GWGCWC 9 cut(s) 2557, 2749, 2836, 3354, 3838, 5387, 6194, 6514, 6895
Alw44I GTGCAC 1 cut(s) 6190
AlwNI CAGNNNCTG 4 cut(s) 2261, 2803, 4609, 7147
Ama87I CYCGRG 2 cut(s) 5862, 6701
Aor13HI TCCGGA 2 cut(s) 664, 5090
Aor51HI AGCGCT 1 cut(s) 5849
ApaI GGGCCC 1 cut(s) 6156
ApaLI GTGCAC 1 cut(s) 6190
AseI ATTAAT 2 cut(s) 1131, 5741
Asp700I GAANNNNTTC 6 cut(s) 1035, 5086, 5247, 5904, 6872, 6983
Asp718I GGTACC 1 cut(s) 5501
AsuC2I CCSGG 3 cut(s) 1314, 1608, 4666
AsuII TTCGAA 1 cut(s) 237
AsuNHI GCTAGC 1 cut(s) 7378
AvaI CYCGRG 2 cut(s) 5862, 6701
AvaII GGWCC 9 cut(s) 2587, 3985, 4499, 4612, 5146, 5350, 5948, 6384, 7144
BaeGI GKGCMC 2 cut(s) 6156, 6194
BamHI GGATCC 3 cut(s) 1531, 1972, 3848
BanI GGYRCC 1 cut(s) 5501
BanII GRGCYC 5 cut(s) 2557, 5032, 5387, 6156, 6514
BarI GAAGNNNNNNTAC 2 cut(s) 2847, 2879
BauI CACGAG 1 cut(s) 3837
BbsI GAAGAC 6 cut(s) 1206, 2122, 3003, 4347, 5907, 6287
Bbv12I GWGCWC 9 cut(s) 2557, 2749, 2836, 3354, 3838, 5387, 6194, 6514, 6895
BbvCI CCTCAGC 2 cut(s) 3213, 3397
BceAI ACGGC 5 cut(s) 2429, 2876, 2948, 3844, 4469
BcgI CGANNNNNNTGC 2 cut(s) 1358, 1392
BciVI GTATCC 5 cut(s) 2149, 3432, 3630, 3633, 6163
BclI TGATCA 2 cut(s) 3604, 5707
BcnI CCSGG 3 cut(s) 1314, 1608, 4666
BcuI ACTAGT 3 cut(s) 452, 1358, 4979
BfmI CTRYAG 4 cut(s) 1665, 2636, 4180, 5657
BfoI RGCGCY 2 cut(s) 5851, 7192
BfrI CTTAAG 2 cut(s) 4154, 4987
BfuAI ACCTGC 1 cut(s) 6234
BfuI GTATCC 5 cut(s) 2149, 3432, 3630, 3633, 6163
BglI GCCNNNNNGGC 1 cut(s) 1934
BglII AGATCT 3 cut(s) 952, 4429, 6098
BlpI GCTNAGC 1 cut(s) 2148
BmcAI AGTACT 2 cut(s) 2107, 7356
Bme18I GGWCC 9 cut(s) 2587, 3985, 4499, 4612, 5146, 5350, 5948, 6384, 7144
BmeRI GACNNNNNGTC 3 cut(s) 3772, 4594, 6738
BmeT110I CYCGRG 2 cut(s) 5862, 6701
BmiI GGNNCC 8 cut(s) 1533, 1974, 3850, 5031, 5503, 6154, 6155, 6385
BmrI ACTGGG 5 cut(s) 2587, 3260, 3744, 5105, 7144
BmtI GCTAGC 1 cut(s) 7382
BmuI ACTGGG 5 cut(s) 2587, 3260, 3744, 5105, 7144
BpiI GAAGAC 6 cut(s) 1206, 2122, 3003, 4347, 5907, 6287
BplI GAGNNNNNCTC 6 cut(s) 8, 40, 4161, 4193, 4993, 5025
BpmI CTGGAG 4 cut(s) 1742, 2568, 5560, 6528
Bpu10I CCTNAGC 2 cut(s) 3213, 3397
Bpu1102I GCTNAGC 1 cut(s) 2148
Bpu14I TTCGAA 1 cut(s) 237
BpuEI CTTGAG 7 cut(s) 1208, 2505, 4610, 5841, 6458, 6566, 6686
BpuMI CCSGG 3 cut(s) 1314, 1608, 4666
Bsa29I ATCGAT 1 cut(s) 321
BsaAI YACGTR 1 cut(s) 5036
BsaBI GATNNNNATC 5 cut(s) 1152, 2973, 4641, 5070, 5743
BsaHI GRCGYC 1 cut(s) 5057
BsaI GGTCTC 1 cut(s) 6693
BsaWI WCCGGW 3 cut(s) 664, 5049, 5090
BsaXI ACNNNNNCTCC 6 cut(s) 942, 972, 4011, 4041, 6937, 6967
Bse118I RCCGGY 1 cut(s) 6546
Bse3DI GCAATG 6 cut(s) 1755, 2760, 4578, 5480, 5700, 7379
Bse8I GATNNNNATC 5 cut(s) 1152, 2973, 4641, 5070, 5743
BseAI TCCGGA 2 cut(s) 664, 5090
BseCI ATCGAT 1 cut(s) 321
BseJI GATNNNNATC 5 cut(s) 1152, 2973, 4641, 5070, 5743
BseMI GCAATG 6 cut(s) 1755, 2760, 4578, 5480, 5700, 7379
BsePI GCGCGC 1 cut(s) 5644
BseRI GAGGAG 5 cut(s) 368, 520, 983, 1921, 2565
BseSI GKGCMC 2 cut(s) 6156, 6194
BseYI CCCAGC 2 cut(s) 4370, 4825
BsgI GTGCAG 2 cut(s) 4434, 5477
Bsh1236I CGCG 7 cut(s) 35, 765, 1511, 3166, 5646, 6094, 6739
Bsh1285I CGRYCG 1 cut(s) 4598
BshNI GGYRCC 1 cut(s) 5501
BshVI ATCGAT 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 4598
BsiHKAI GWGCWC 9 cut(s) 2557, 2749, 2836, 3354, 3838, 5387, 6194, 6514, 6895
BsiHKCI CYCGRG 2 cut(s) 5862, 6701
BsiSI CCGG 9 cut(s) 665, 1060, 1313, 1607, 4502, 4666, 5050, 5091, 6547
BslFI GGGAC 4 cut(s) 2745, 2888, 3398, 6397
BsmBI CGTCTC 6 cut(s) 5, 35, 145, 201, 1913, 7205
BsmFI GGGAC 4 cut(s) 2745, 2888, 3398, 6397
BsmI GAATGC 4 cut(s) 780, 5018, 6904, 7271
Bso31I GGTCTC 1 cut(s) 6693
BsoBI CYCGRG 2 cut(s) 5862, 6701
Bsp119I TTCGAA 1 cut(s) 237
Bsp120I GGGCCC 1 cut(s) 6152
Bsp13I TCCGGA 2 cut(s) 664, 5090
Bsp1407I TGTACA 1 cut(s) 3510
Bsp1720I GCTNAGC 1 cut(s) 2148
Bsp19I CCATGG 5 cut(s) 1293, 2142, 5346, 6247, 6896
BspDI ATCGAT 1 cut(s) 321
BspEI TCCGGA 2 cut(s) 664, 5090
BspFNI CGCG 7 cut(s) 35, 765, 1511, 3166, 5646, 6094, 6739
BspHI TCATGA 2 cut(s) 3238, 4744
BspLI GGNNCC 8 cut(s) 1533, 1974, 3850, 5031, 5503, 6154, 6155, 6385
BspMAI CTGCAG 4 cut(s) 1669, 2640, 4184, 5661
BspMI ACCTGC 1 cut(s) 6234
BspOI GCTAGC 1 cut(s) 7382
BspQI GCTCTTC 1 cut(s) 4741
BspT104I TTCGAA 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 5501
BspTI CTTAAG 2 cut(s) 4154, 4987
BspTNI GGTCTC 1 cut(s) 6693
BsrDI GCAATG 6 cut(s) 1755, 2760, 4578, 5480, 5700, 7379
BsrFI RCCGGY 1 cut(s) 6546
BsrGI TGTACA 1 cut(s) 3510
BssAI RCCGGY 1 cut(s) 6546
BssHII GCGCGC 1 cut(s) 5644
BssNI GRCGYC 1 cut(s) 5057
BssSI CACGAG 1 cut(s) 3837
BssT1I CCWWGG 8 cut(s) 254, 1293, 2142, 4718, 5346, 6247, 6366, 6896
Bst2BI CACGAG 1 cut(s) 3837
Bst6I CTCTTC 8 cut(s) 204, 1673, 1749, 2307, 3432, 4432, 4627, 4741
BstACI GRCGYC 1 cut(s) 5057
BstAFI CTTAAG 2 cut(s) 4154, 4987
BstAPI GCANNNNNTGC 7 cut(s) 898, 1745, 2200, 3209, 5342, 5438, 5787
BstAUI TGTACA 1 cut(s) 3510
BstBAI YACGTR 1 cut(s) 5036
BstBI TTCGAA 1 cut(s) 237
BstDSI CCRYGG 6 cut(s) 1293, 2142, 2964, 5346, 6247, 6896
BstENI CCTNNNNNAGG 2 cut(s) 1678, 2651
BstFNI CGCG 7 cut(s) 35, 765, 1511, 3166, 5646, 6094, 6739
BstH2I RGCGCY 2 cut(s) 5851, 7192
BstMCI CGRYCG 1 cut(s) 4598
BstSFI CTRYAG 4 cut(s) 1665, 2636, 4180, 5657
BstSLI GKGCMC 2 cut(s) 6156, 6194
BstUI CGCG 7 cut(s) 35, 765, 1511, 3166, 5646, 6094, 6739
BstV2I GAAGAC 6 cut(s) 1206, 2122, 3003, 4347, 5907, 6287
BstX2I RGATCY 8 cut(s) 952, 1303, 1531, 1972, 2622, 3848, 4429, 6098
BstXI CCANNNNNNTGG 1 cut(s) 4719
BstYI RGATCY 8 cut(s) 952, 1303, 1531, 1972, 2622, 3848, 4429, 6098
Bsu15I ATCGAT 1 cut(s) 321
BsuI GTATCC 5 cut(s) 2149, 3432, 3630, 3633, 6163
BsuTUI ATCGAT 1 cut(s) 321
BtgI CCRYGG 6 cut(s) 1293, 2142, 2964, 5346, 6247, 6896
BtgZI GCGATG 1 cut(s) 5141
BtsI GCAGTG 2 cut(s) 3526, 3838
BveI ACCTGC 1 cut(s) 6234
CaiI CAGNNNCTG 4 cut(s) 2261, 2803, 4609, 7147
CciI TCATGA 2 cut(s) 3238, 4744
Cfr10I RCCGGY 1 cut(s) 6546
ClaI ATCGAT 1 cut(s) 321
CpoI CGGWCCG 1 cut(s) 3985
CseI GACGC 8 cut(s) 2621, 2783, 3155, 3172, 3478, 4914, 5397, 6728
CsiI ACCWGGT 3 cut(s) 1963, 3406, 6130
CspCI CAANNNNNGTGG 4 cut(s) 637, 672, 4477, 4512
CspI CGGWCCG 1 cut(s) 3985
DraI TTTAAA 4 cut(s) 1809, 2229, 3630, 6259
DraIII CACNNNGTG 3 cut(s) 898, 1745, 3410
DriI GACNNNNNGTC 3 cut(s) 3772, 4594, 6738
EaeI YGGCCR 3 cut(s) 2889, 5425, 7255
Eam1104I CTCTTC 8 cut(s) 204, 1673, 1749, 2307, 3432, 4432, 4627, 4741
Eam1105I GACNNNNNGTC 3 cut(s) 3772, 4594, 6738
EarI CTCTTC 8 cut(s) 204, 1673, 1749, 2307, 3432, 4432, 4627, 4741
EciI GGCGGA 3 cut(s) 4520, 5940, 6233
Ecl136II GAGCTC 3 cut(s) 2555, 5385, 6512
Eco130I CCWWGG 8 cut(s) 254, 1293, 2142, 4718, 5346, 6247, 6366, 6896
Eco24I GRGCYC 5 cut(s) 2557, 5032, 5387, 6156, 6514
Eco31I GGTCTC 1 cut(s) 6693
Eco32I GATATC 3 cut(s) 1067, 1903, 3249
Eco47I GGWCC 9 cut(s) 2587, 3985, 4499, 4612, 5146, 5350, 5948, 6384, 7144
Eco47III AGCGCT 1 cut(s) 5849
Eco53kI GAGCTC 3 cut(s) 2555, 5385, 6512
Eco57I CTGAAG 8 cut(s) 1220, 2451, 3413, 4007, 4368, 5226, 6859, 6906
Eco88I CYCGRG 2 cut(s) 5862, 6701
EcoICRI GAGCTC 3 cut(s) 2555, 5385, 6512
EcoNI CCTNNNNNAGG 2 cut(s) 1678, 2651
EcoO109I RGGNCCY 2 cut(s) 6153, 7144
EcoRI GAATTC 4 cut(s) 2642, 5086, 6631, 7013
EcoRV GATATC 3 cut(s) 1067, 1903, 3249
EcoT14I CCWWGG 8 cut(s) 254, 1293, 2142, 4718, 5346, 6247, 6366, 6896
EcoT22I ATGCAT 5 cut(s) 1494, 1707, 5360, 6268, 6272
EcoT38I GRGCYC 5 cut(s) 2557, 5032, 5387, 6156, 6514
ErhI CCWWGG 8 cut(s) 254, 1293, 2142, 4718, 5346, 6247, 6366, 6896
Esp3I CGTCTC 6 cut(s) 5, 35, 145, 201, 1913, 7205
FaqI GGGAC 4 cut(s) 2745, 2888, 3398, 6397
FauI CCCGC 2 cut(s) 565, 758
FauNDI CATATG 2 cut(s) 1707, 6060
FbaI TGATCA 2 cut(s) 3604, 5707
FblI GTMKAC 2 cut(s) 1074, 5728
FriOI GRGCYC 5 cut(s) 2557, 5032, 5387, 6156, 6514
FspI TGCGCA 1 cut(s) 2514
GsaI CCCAGC 2 cut(s) 4374, 4829
GsuI CTGGAG 4 cut(s) 1742, 2568, 5560, 6528
HaeII RGCGCY 2 cut(s) 5851, 7192
HapII CCGG 9 cut(s) 665, 1060, 1313, 1607, 4502, 4666, 5050, 5091, 6547
HgaI GACGC 8 cut(s) 2621, 2783, 3155, 3172, 3478, 4914, 5397, 6728
Hin1I GRCGYC 1 cut(s) 5057
HincII GTYRAC 4 cut(s) 2860, 5595, 7089, 7215
HindII GTYRAC 4 cut(s) 2860, 5595, 7089, 7215
HindIII AAGCTT 4 cut(s) 1232, 2813, 4466, 5492
HpaI GTTAAC 1 cut(s) 7215
HpaII CCGG 9 cut(s) 665, 1060, 1313, 1607, 4502, 4666, 5050, 5091, 6547
Hpy99I CGWCG 1 cut(s) 5059
HpyCH4IV ACGT 9 cut(s) 80, 1599, 2862, 3280, 5035, 5057, 5376, 5974, 7055
HpySE526I ACGT 9 cut(s) 80, 1599, 2862, 3280, 5035, 5057, 5376, 5974, 7055
Hsp92I GRCGYC 1 cut(s) 5057
Kpn2I TCCGGA 2 cut(s) 664, 5090
KpnI GGTACC 1 cut(s) 5505
Ksp22I TGATCA 2 cut(s) 3604, 5707
KspAI GTTAAC 1 cut(s) 7215
LguI GCTCTTC 1 cut(s) 4741
MabI ACCWGGT 3 cut(s) 1963, 3406, 6130
MaeII ACGT 9 cut(s) 80, 1599, 2862, 3280, 5035, 5057, 5376, 5974, 7055
MfeI CAATTG 5 cut(s) 2070, 2480, 5421, 5711, 5967
MflI RGATCY 8 cut(s) 952, 1303, 1531, 1972, 2622, 3848, 4429, 6098
MluI ACGCGT 1 cut(s) 3164
MmeI TCCRAC 6 cut(s) 168, 705, 726, 3341, 3414, 4629
Mph1103I ATGCAT 5 cut(s) 1494, 1707, 5360, 6268, 6272
MroI TCCGGA 2 cut(s) 664, 5090
MroXI GAANNNNTTC 6 cut(s) 1035, 5086, 5247, 5904, 6872, 6983
MslI CAYNNNNRTG 9 cut(s) 683, 1497, 1518, 1896, 3762, 5023, 5265, 5722, 7208
MspA1I CMGCKG 7 cut(s) 773, 2089, 2441, 3200, 4135, 4391, 7028
MspCI CTTAAG 2 cut(s) 4154, 4987
MspI CCGG 9 cut(s) 665, 1060, 1313, 1607, 4502, 4666, 5050, 5091, 6547
MunI CAATTG 5 cut(s) 2070, 2480, 5421, 5711, 5967
Mva1269I GAATGC 4 cut(s) 780, 5018, 6904, 7271
MvnI CGCG 7 cut(s) 35, 765, 1511, 3166, 5646, 6094, 6739
NciI CCSGG 3 cut(s) 1314, 1608, 4666
NcoI CCATGG 5 cut(s) 1293, 2142, 5346, 6247, 6896
NdeI CATATG 2 cut(s) 1707, 6060
NheI GCTAGC 1 cut(s) 7378
NlaIV GGNNCC 8 cut(s) 1533, 1974, 3850, 5031, 5503, 6154, 6155, 6385
NmeAIII GCCGAG 3 cut(s) 1913, 4520, 5453
NsbI TGCGCA 1 cut(s) 2514
NsiI ATGCAT 5 cut(s) 1494, 1707, 5360, 6268, 6272
NspV TTCGAA 1 cut(s) 237
PaeI GCATGC 2 cut(s) 6270, 6674
PaeR7I CTCGAG 1 cut(s) 5862
PagI TCATGA 2 cut(s) 3238, 4744
PauI GCGCGC 1 cut(s) 5644
PciI ACATGT 5 cut(s) 593, 650, 1180, 7209, 7389
PciSI GCTCTTC 1 cut(s) 4741
PctI GAATGC 4 cut(s) 780, 5018, 6904, 7271
PdmI GAANNNNTTC 6 cut(s) 1035, 5086, 5247, 5904, 6872, 6983
PflFI GACNNNGTC 1 cut(s) 5056
PflMI CCANNNNNTGG 3 cut(s) 1299, 3284, 4490
PfoI TCCNGGA 2 cut(s) 6941, 7122
Ppu21I YACGTR 1 cut(s) 5036
PpuMI RGGWCCY 1 cut(s) 7144
PscI ACATGT 5 cut(s) 593, 650, 1180, 7209, 7389
PshBI ATTAAT 2 cut(s) 1131, 5741
Psp124BI GAGCTC 3 cut(s) 2557, 5387, 6514
Psp5II RGGWCCY 1 cut(s) 7144
PspFI CCCAGC 2 cut(s) 4370, 4825
PspN4I GGNNCC 8 cut(s) 1533, 1974, 3850, 5031, 5503, 6154, 6155, 6385
PspOMI GGGCCC 1 cut(s) 6152
PspPPI RGGWCCY 1 cut(s) 7144
PspXI VCTCGAGB 1 cut(s) 5862
PsrI GAACNNNNNNTAC 2 cut(s) 3865, 3897
PstI CTGCAG 4 cut(s) 1669, 2640, 4184, 5661
PstNI CAGNNNCTG 4 cut(s) 2261, 2803, 4609, 7147
PsuI RGATCY 8 cut(s) 952, 1303, 1531, 1972, 2622, 3848, 4429, 6098
PsyI GACNNNGTC 1 cut(s) 5056
PteI GCGCGC 1 cut(s) 5644
PvuII CAGCTG 6 cut(s) 773, 2089, 2441, 3200, 4135, 7028
RseI CAYNNNNRTG 9 cut(s) 683, 1497, 1518, 1896, 3762, 5023, 5265, 5722, 7208
Rsr2I CGGWCCG 1 cut(s) 3985
RsrII CGGWCCG 1 cut(s) 3985
SacI GAGCTC 3 cut(s) 2557, 5387, 6514
SapI GCTCTTC 1 cut(s) 4741
ScaI AGTACT 2 cut(s) 2107, 7356
SexAI ACCWGGT 3 cut(s) 1963, 3406, 6130
SfcI CTRYAG 4 cut(s) 1665, 2636, 4180, 5657
Sfr274I CTCGAG 1 cut(s) 5862
SfuI TTCGAA 1 cut(s) 237
SinI GGWCC 9 cut(s) 2587, 3985, 4499, 4612, 5146, 5350, 5948, 6384, 7144
SlaI CTCGAG 1 cut(s) 5862
SmiMI CAYNNNNRTG 9 cut(s) 683, 1497, 1518, 1896, 3762, 5023, 5265, 5722, 7208
SpeI ACTAGT 3 cut(s) 452, 1358, 4979
SphI GCATGC 2 cut(s) 6270, 6674
SspI AATATT 2 cut(s) 556, 4764
SstI GAGCTC 3 cut(s) 2557, 5387, 6514
StyI CCWWGG 8 cut(s) 254, 1293, 2142, 4718, 5346, 6247, 6366, 6896
TaiI ACGT 9 cut(s) 83, 1602, 2865, 3283, 5038, 5060, 5379, 5977, 7058
TaqII GACCGA 1 cut(s) 4584
TatI WGTACW 5 cut(s) 2105, 3510, 4187, 6498, 7354
TauI GCSGC 6 cut(s) 38, 1348, 2052, 4120, 6800, 7260
TspGWI ACGGA 9 cut(s) 29, 1895, 2729, 2892, 3380, 3727, 5459, 5612, 5854
Tth111I GACNNNGTC 1 cut(s) 5056
Van91I CCANNNNNTGG 3 cut(s) 1299, 3284, 4490
Vha464I CTTAAG 2 cut(s) 4154, 4987
VneI GTGCAC 1 cut(s) 6190
VpaK11BI GGWCC 9 cut(s) 2587, 3985, 4499, 4612, 5146, 5350, 5948, 6384, 7144
VspI ATTAAT 2 cut(s) 1131, 5741
XagI CCTNNNNNAGG 2 cut(s) 1678, 2651
XcmI CCANNNNNNNNNTGG 1 cut(s) 6227
XhoI CTCGAG 1 cut(s) 5862
XmiI GTMKAC 2 cut(s) 1074, 5728
XmnI GAANNNNTTC 6 cut(s) 1035, 5086, 5247, 5904, 6872, 6983
ZraI GACGTC 1 cut(s) 5058
ZrmI AGTACT 2 cut(s) 2107, 7356
Zsp2I ATGCAT 5 cut(s) 1494, 1707, 5360, 6268, 6272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.