MD15G1113500.v1.1

phosphatidylinositol-3-phosphate binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005768
Physical Location & Seq
Forward (+)
7977684 .. 8005564
27881 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1113500.v1.1.491

Sequence Viewer

Length: 7704 bp
ATGGACAAGGAAACCGAAATCCTCTCCCGCCTGGCCGCGAACCACCTCTATCTCGCCCAATTCGAGCCGCTCCGGGCGGTTCTCATCGCCCTCCGAGCCCGTAACCCCGAGCTCGCTCTCGCTGTGCTCCAGACCATCGTCGCGCAGTCTGGCCGGTTCGAGAACGTTCTCTGGTCGCAATCCTGTCCGTCGCCGGCTCTCCTCACTTACTTGTCGACCGTCGAGCTCTTGCAATTCGATAATGCGTCGTCAGCGTGGAGCTTCGATCCCGAAACGCTGCGGTTGCGAGCCGAGTTCTTATTGCTAGTCCAGCAACTGATCGACCGGGTATCTGAGAGTTTGCGGAAGGATTTCGATTTGGAGGGCATAGAGAAAGAGAAGGAGAAAGAGGGTTTGGGCGAGAGCGAGAGTTTCGAAGAGCGCGCGGAGTTGTTGGAGAAGAGTGATGATACTAGCAAGGATTTAAGAGATGGCAGTGGGGAATTAGATAGCTGTGTGAAAGTTTTGGATAGGGTGTTGGAATTGGGGGTCAAGAGGTTGAAGCCGGGTTCTGTGGCGGTAGAGTCTGCTGATGCTGATGTTGGGAGTGGAAATGAGGCGCCAAGCGTTGTACCGATTGAGGAAGGCGAATTGATGTGTTTAAGGAGTGTAGTTTGGGAGTACCGAGATGTTTTCGAGGCGTTGTGTTGGAATATACAGAGCCAAGTGAGGGGGTGGGAGGGAGATGATTCTTCGGGTTTAGCTCTAACGGTGAGGAGGGACGAAAATGCAGGGGGAACCTCCGAGGAGGATTTGAAAGTTCTTGGTTTGATACAGAGGAGTGTTCAGTTAGCTCACTTAGATGCTATGAAAGAGGGCATGAAGAATGGTGATGTCGATGGAGTGGTTTCGCGGATTCGTTTTCTTCAACTTGATTATGGAGTGGAGGAGAACAAGTATCGCATGGTTCTGCAAGATCTTCTCAAGATGGTGTCATCAGGAAAGGAGGGATATGGAGATTCCTGGCGTGACATGCGAGAAAAGTTACTGGGGATTTATGGTGCTGCTCTTGCATCAAGTTCTGGACGTCTTGTTGAAATGATACATGTTCTTCAAGATGAGTTGCTCTCAGAAGAGATTGAGAAGTACAGATCTCTTGATGACAACCAGATTCCACCACCTCTTGAACGTCTTCAAAGATATCTTGCTGGGTTGAATCCTGAGGCAGATGTTTCTGATCAAACTTGTACCTTCAACACTGTAGCCGGATTTTGCATGAGAGACATGTACCATTATGCCCGTGTTTCTGGTTTACATGTCCTTGAATGTGTCATGGATACTGCTTTGTCTGCTGTGAAGAGGGAGCAGCTTCAAGAAGCTAGCAATATTCTTTTGTTGTTTCCCCGACTTCAGCCCCTGGTAGCTGCCATGGGTTGGGATTTATTATCTGGCAAGACTGCAGCACGAAGGAAATTAATGCAACTGCTGTGGAGAAGCAAGTCACAGGTTTTACGCCTGGAAGAATCTTCTCTTTATAGTAATCAATCGGATGAGGTATCTTGTGTGGAATATCTCTGTGATTCTCTTTGTTATCGGCTTGATCTTGCCTCTTTTGTTGCCTGTGTCAATTCTGGTAAATCATGGAATTCAAAATCATCATTGATGTTGTCTGCTAAAGAACAACTAGCCTCCAGTAATGAAGATGCTGAGAATGATCCTTTTGTCGAGAATTTCGTACTGGAAAGGCTTTCTGTTCAAAGTCCTCTTCGGGTATTGTTTGATGTTGTTCCAGGCATAAAGTTTCAGGAGGCCATTGAATTGATAAGCATGCAGCCAATTGCATCAACTTTAGAAGCCTGGAAGAGGATGCAGGATATTGAACTCATGCATATGCGCTATGCTCTAGATTCAGCTGTTCTTGCAGTTGGCGTGATGGAAAGGAGTATTACTGGTGAAAGTGACGGTCTTTATCAAGTTGCATTTAGCCATCTGAAAGATCTGCAGAACCATTTGGAGGCTGTCACCGATATCCCTCGCAAGATCATGATGGCAAATGTCATAATTTCACTTTTACATATGGATGATCTTTCTCTCAATTTGGATCATTGTGCTTCACCGGGGAGCTCTTCTGGATCACACCACTATGCTTCAGAACAAACTGATGAAACATGTGAAGGGGGAAATGAATTGGTTATATCCTTTACTGGGAAACTACTATCAATATTAAATCACTGTCTTCCGTCAACTGTAGCTGAACTAGATAATGCTCTAAGTGAAGGCATTAGTAGGGATGGAAGACAAGCGTTGGAGTGGAGAGCATCAATTGCTAAACATTTCATTGAAGAGTGGGAATGGCGGCTGTCAATTTTGCAGCGTCTTCTTCCATTATCTGAACGTCAGTGGCAGTGGAAGGAGGCATTAACTGTATTACGTGCCGCACCATCTAAGCTACTTAACCTGTGCATGCAAAGAGCAAAGTATGACATTGGCGAAGAAGCAGTGCATCGCTTTTCATTGTCTGCAGAAGATAAAGCTACTCTTGAATTAGCTGAGTGGGTGGATCGTGCTGTCAGAAGACAATCTGTAGAAGATGTAGTCTCTCGTGCTACTGATGGTGGGACTTCTACAATACAAGATCTGGATTTTTCTTCATTACGCTCCCAGTTAGGTCCCTTGGCTGCAATTCTTCTTTGTGTCGATGCTGCTGCTACTTCTGCTAGGTCTGCAAAGATAAATATCATGAGCTTGGAAGAAAGTAAAGTTTGCCTCGATTGGTATCATTGGATTGTTATTGGAAACCGTCTCTGGATGTATTCCAGAACGTTCGCTCAAGTTATGCTGTCTGAGATATATCCAGGAGTTTCTCCAAAGATAGGCTCCATGTACTGGGATCAGATCCTTGAAGTGGCAGTTATATCTGTTTTAAAACGAATTCTGAAGCGTTTACATGAGTTCTTGGATCAGGATAATTCTCCAGCCCTTCAGGTCACTTTGTCTGGGGAGAACATCATTTCTTCACCAAAGGAATTCCAGAGACTAGGACAACGGGAGCGTGTTCTCGATATGCTACATCATATGATTGAGGATGCTCACAAGGGAAAGCGGCAGTTTCTCAGTGGTAAGCTCCATAATCTTGCAAGAGCCATAGCTGATGAAGAAACAGAGCTAAATTCTTTTAAAGGAGAAGGCCCATCTGGTGACCCGAAGGTTCTTTATGATCTCGACAAAGACGGAGTTCTTGGACTTGGGCTAAGAGTTTCCAAACAGATACCATCAAATTCCGCAGTTGGAGAAACTACATTGCAGCCTGTTGGCTATGATGTAAAGGACAATGGGAAGAGATTATTTGGTCCAATAAGTACCAAACCCATGACATATCTTTCACAATTTATTCTCCATATTGCTGCAATTGGAGACATAGTTGATGGAACAGATACAACTCACGATTTCAACTTTTTTTCACTCGTGTATGAGTGGCCCAAGGATCTTTTAACTCGTCTTGTCTTTGAACGAGGCAGCACTGATGCAGCTGCTAAGGTAGCTGAGATCATGTGTGCAGATTTTGTCCATGAAGTAATTTCAGCATGTGTACCTCCAGTTTATCCCCCACGATCTGGTCATGGATGGGCCTGCATTCCAGTCTTTCCCACCTTCCCTAAAAGTGGCTCAGAAAATAAAGTATTATCCCCCTCTTTCAAAGATGCTAAGCCTAATAGTTACTGCCGGTCCTCATCCTTGCCCGGAATCCCTCTATACCCTCTTCAGTTAGATGTCATTAAACATTTGGTTAAGCTATCCCCAGTGAGGGCAGTCTTAGCATGTGTTTTTGGGAGTACAATTTTGTACAATGGCAGTGATTCTTCGATCTCAAGCTCCTTGGATGGTGGATCGCTGCAGGCACCTGATGTTGATCGGTTGTTTTATGAGTTTGCTCTTGATCAGTCGGAGAGGTTTCCAACTTTAAACCGATGGATACAAATGCAAACTAACCTTCATCGAGTTTCTGAGTTTGCTGTAACAATTAAGCAAACTGCCGATGGTGGCGAGGCTAGGGCTGAAGCAAGAGCTATCAAGAGATTACGCGAGCTTGACAGTGATACTGAGTCAGAAGTTGATGACATTGTTGGTAGCAGTAGTGTTTCAACAGCTTTACCAGACCTCAATGACCAAGGTGGTGCAGCTACTGAACCTTGGGATGACTCTTCAAAATCTGATGTTGCTGAGCTTGATACTTCAGTTTTCCTTTCTTTTGATTGGGAAAATGAAGAACCGTATGAGAAAGCTGTACAGAGATTGATAGAAGAAGGTAAACTCATGGATGCTCTTGCACTGTCTGATCGCTATCTACGTAATGGAGCTTCAGATCATTTACTTCAGCTAATCATTGAAAGTGGGGAAGAAACTCGGTCAGTCCCTGTACAATCTCACGTTTATGGAGGAAATAGCATATGGAGCAATAGTTGGCAGTATTGCCTGCGGTTGAACGATAAACAAGTGGCTGCTAGACTTGCTCTCAAGTATATGCATAGATGGGAACTGGATGCTGCTTTGGATGTTCTCACAATGTGCAGCTGCCACCTACCTCAAAGTGATTCCATTAGGAAGGAGGTCATGCATATGAGACAAGCTCTGCAGAGGTACAGCCACATTATAAGTGCAGATGAGCACTTTAGCAGCTGGCAAGAGGTTGAAGCAGAGTGTAAAGAAGATCCTGAGGGCTTGGCACTCAGATTAGCTGGAAAAGGAGCTGTTTCCGCTGCTTTAGAAGTGGCTGAAAGTGCAGGATTATCCATAGATTTGCGGAGAGAATTGCAGGGCCGGCAGCTTGTGAAACTTTTGACTGCAGATCCTCTCAGTGGTGGAGGTCCAGCAGAAGCTTCGCGGTTTCTATCTTCTCTACGTGAATCAGATGATGCTCTACCAGTTGCCATGGGTGCAATGCAGCTTTTACCTGATTTACGGTCAAAGCAGCTTCTTGTTCATTTCTTCCTGAAGCGAAGAGAAGGAAATCTTTCAGATGTTGAGGTTTCTCGGCTTAACTCATGGGCTTTAGGTCTTCGTGTTCTGGCTGCCTTGCCATTGCCATGGCAGCAAAGATGTTCTTCCTTGCATGAGCACCCTCACCTGATACTTGAGGTTCTTCTGATGAGGAAACAGCTACAGTCAGCTGCTTTGATTCTCAAAGAATTTCCTTTGTTGAGAGACAATAATGTCATTATTGCCTATGCTGCTAAAGCTATTGCTATCGGTATTAGCTCTCCCCCGAGAGAATATCGAGTATCTGTTTCAGGGACAAGATCAAAGCAGAAAACAAGAACAGGTGCACCTGTGAGGTCTTCTTTCACGAGCAGTCTAAGTAACCTGCAAAAGGAGGCTCGCAGAGCTTTCTCTTGGGCTCCACGAAATACCGGAGACAGGGCCGCCCCAAAAGATGTTTACCGTAAAAGAAAGAGTTCAGGTTTGACGCCATCAGAAAAAGTAGCTTGGGAGGCCATGGCTGGTATCCAAGAGGATCGTGCTTCAACATATTCTGTTGATGGGCAAGAACGTCTTCCTGCTATTTCCATTTCTGAAGAATGGATGCTAACTGGTGATGCAACGAAGGATGAAGTTGTTCGTGCATCTCACCGTTATGAAAGTGCCCCTGACATTACTCTTTTCAAGGCACTGATTTCTCTATGTTCTGATGATTCTGTCTCTGCAAAAAGTGCATTGGATTTGTGTGTTAAACAGATGAAGAATGTGCTGAGCTCCCAACAATTGCCTGAGAATGCATCTATGGAAATAATTGGTCGAGCATATCATGCCACAGAGACGCTTGTACAGGGGCTACTTTACGCTAAATCCCTGCTGAGAAGGATTGTGGGTGGTAATGATTTGTCAAGTAATTCTGAAAGAAGCAGGGACATTGATGATGCATCCTCTGATGCTGGTAGCTCTAGTGTGGGCAGTCAGTCCACAGATGAGTTATCTGAAGTGTTGTCGCAAGCAGACATTTGGCTAGGACGCGCTGAGCTGCTCCAAAGCCTTCTGGGATCTGGAATAGCTGCTTCTCTAGATGATATTGCCGATAAAGAGTCATCTGCCCGTCTCCGTGATAGGCTGATTGTAGATGAACGGTACAGCATGGCTGTATATACATGCAAAAAGTGCAAGATTGATGTTGTCCCTGTTTGGAATGCATGGGGACATGCTTTGATTCGGATGGAGCACTATGCCCAGGCTCGTGTCAAGTTCAAGCAAGCTCTCCAACTGTACAAAGCTGATCCTGCTCCTGTCATTCTTGAGATCATCAATACAATCGAAGGAGGTCCGCCTGTTGATGTTTCAGCTGTTCGTTCCATGTATGAACATTTGGCTAAAAGTGCACCGACAATCTTGGATGATTCTCTTTCTGCTGATGCATATCTCAACGTATTGTACATGCCTTCGACATTTCCTCGATCAGAGCGATCCAGGCGTTCTCATGAATCTGCGAATAGTAATTCCACTCATATTTCAGACTTTGAAGATGGACCTCGCAGCAACTTAGACAGCATACGATATGTTGAGTGTGTCAACTATTTACAAGAATATGCACGGCAGCATTTGCTCAATTTCATGTTTAGGCATGGTCATTATAATGATGCATGCATGCTGTTCTTTCCTCCAAATGCTGTTCCCCCACCTTCTCAGCCATCAACAGTGGGGGCAGCATCCTCATCTTCATCCCCTCAAAGGCCAGACCCTTTGGGAACTGATTATGGGACCATTGACGATTTGTGTGATTTATGTGTTGGTTATGGTGCAATGGCTGTTTTAGAAGAAGTAATTTCAACAAGAATGTCATCTGAAAACCTGCAAGATGTAGCAGTAAGCCAGTATACAGCTGCGGCCCTTTCCCGTATTTGCATCTACTGTGAAACTCACAGGCATTTCAATTATTTGTACAAGTTTCAGGTTATTAAGAAGGACCATGTTGCTGCTGGACTTTGCTGCATCCAATTGTTTATGAACTCATCTTTGCAAGAGGAAGCTATAAAACATTTGGAAAATGCCAAGATGCACTTTGATGAAGCGTTATCAGCACGATCCAAAGGTGGGGATCCTACTAAGCTTGTGACGAAGGGTGTTAGAGGAAAAAGTGCCTCTGAAAAGCTAACTGAAGAAGGACTTGTCAAGTTTTCGGCGCGGGTATCGATTCAGGTTGAAGTTGTGAGATCGTATAATGATGCGGATGGACCACATTGGAAACATTCACTTTTCGGAAATCCAAATGACCATGAAACATTCAGGAGAAGGTGCAAAATAGCTGAATCTCTTGTGGAAAAGAATTTTGATTTGGCTTTCCAAGTGATTTATGAATTCAATCTTCCAGCTGTGGATATATATGCTGGTGTCGCTGCATCACTCGCTGAGAGAAAAAAGGGTAGTCAACTGACAGAATTCTTTAGAAACATAAAAGGAACAATTGATGACGATGACTGGGACCAGGTCTTGGGAGCTGCAATTAATGTATATGCTAACAAACACAAAGAGCGCCCTGACCGTCTCATAGACATGTTGACCAGTAGCCACAGGAAGGTTCTGGCTTGTGTGGTTTGTGGTCGTCTAAAAAGTGCATTCCAAATTGCTTCTCGTAGTGGAAGTGTAGCCGATGTCCAATATGTAGCTCATCAGGCATTACATGCGAATGCACTTCCTGTGCTGGATATGTGTAAACAATGGTTGGCCCAGTACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2568

Amino Acids

285.58

Weight (kDa)

5.37

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ZFYVE26 PF25569 2445 - 2564 2.4e-32 ZFYVE26-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4631, 6600
AasI GACNNNNNNGTC 1 cut(s) 5189
AatII GACGTC 1 cut(s) 1069
Acc36I ACCTGC 2 cut(s) 5349, 6825
AccB1I GGYRCC 2 cut(s) 598, 3877
AccB7I CCANNNNNTGG 4 cut(s) 1413, 2865, 3593, 7456
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 2 cut(s) 215, 6842
AccII CGCG 9 cut(s) 38, 143, 423, 425, 892, 4062, 4861, 5988, 7150
AclI AACGTT 2 cut(s) 165, 2801
AcoI YGGCCR 2 cut(s) 33, 151
AcyI GRCGYC 3 cut(s) 599, 1066, 5444
AdeI CACNNNGTG 1 cut(s) 4545
AflIII ACRYGT 6 cut(s) 1084, 1263, 1294, 2147, 7518, 7698
AjuI GAANNNNNNNTTGG 4 cut(s) 377, 409, 7283, 7315
Alw44I GTGCAC 2 cut(s) 5301, 6345
AlwNI CAGNNNCTG 4 cut(s) 316, 1396, 4163, 4394
Ama87I CYCGRG 2 cut(s) 107, 5242
ApaLI GTGCAC 2 cut(s) 5301, 6345
AseI ATTAAT 2 cut(s) 1454, 7470
Asp700I GAANNNNTTC 6 cut(s) 350, 1170, 4990, 5431, 5529, 5592
AspLEI GCGC 8 cut(s) 145, 423, 425, 601, 1875, 5990, 7150, 7500
AsuC2I CCSGG 5 cut(s) 74, 326, 546, 2097, 3720
AsuII TTCGAA 1 cut(s) 414
AsuNHI GCTAGC 1 cut(s) 1358
AvaI CYCGRG 2 cut(s) 107, 5242
AxyI CCTNAGG 2 cut(s) 1200, 4692
BaeGI GKGCMC 3 cut(s) 5305, 5623, 6349
BaeI ACNNNNGTAYC 2 cut(s) 4200, 4233
BamHI GGATCC 1 cut(s) 7063
BanI GGYRCC 2 cut(s) 598, 3877
BanII GRGCYC 6 cut(s) 100, 114, 228, 2105, 5377, 5732
BarI GAAGNNNNNNTAC 2 cut(s) 4321, 4353
BauI CACGAG 4 cut(s) 2580, 3443, 5323, 6204
BbsI GAAGAC 8 cut(s) 1163, 2207, 2281, 2348, 2560, 5027, 5307, 5522
BceAI ACGGC 1 cut(s) 6575
BcgI CGANNNNNNTGC 4 cut(s) 2666, 2700, 4839, 4873
BciVI GTATCC 3 cut(s) 1309, 3945, 5492
BclI TGATCA 2 cut(s) 1216, 3916
BcnI CCSGG 5 cut(s) 74, 326, 546, 2097, 3720
BfoI RGCGCY 2 cut(s) 602, 7501
BfuAI ACCTGC 2 cut(s) 5349, 6825
BfuI GTATCC 3 cut(s) 1309, 3945, 5492
BglII AGATCT 4 cut(s) 955, 1130, 1975, 2614
BlpI GCTNAGC 4 cut(s) 3684, 4200, 5726, 5991
BmeT110I CYCGRG 2 cut(s) 107, 5242
BmiI GGNNCC 9 cut(s) 600, 778, 2650, 2857, 3879, 5376, 6727, 7065, 7448
BmrI ACTGGG 7 cut(s) 1037, 2193, 2635, 2875, 3773, 7453, 7687
BmtI GCTAGC 1 cut(s) 1362
BmuI ACTGGG 7 cut(s) 1037, 2193, 2635, 2875, 3773, 7453, 7687
BoxI GACNNNNGTC 1 cut(s) 137
BpiI GAAGAC 8 cut(s) 1163, 2207, 2281, 2348, 2560, 5027, 5307, 5522
BplI GAGNNNNNCTC 4 cut(s) 1091, 1123, 4591, 4623
BpmI CTGGAG 4 cut(s) 113, 1654, 2937, 3558
Bpu10I CCTNAGC 1 cut(s) 3513
Bpu1102I GCTNAGC 4 cut(s) 3684, 4200, 5726, 5991
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 6 cut(s) 947, 2793, 3832, 4478, 5132, 6284
BpuMI CCSGG 5 cut(s) 74, 326, 546, 2097, 3720
Bsa29I ATCGAT 1 cut(s) 7157
BsaAI YACGTR 3 cut(s) 2411, 4328, 4880
BsaBI GATNNNNATC 5 cut(s) 2714, 2754, 3888, 4320, 6384
BsaHI GRCGYC 3 cut(s) 599, 1066, 5444
BsaWI WCCGGW 1 cut(s) 5387
BsaXI ACNNNNNCTCC 6 cut(s) 920, 950, 3384, 3414, 4423, 4453
Bse118I RCCGGY 4 cut(s) 153, 193, 3702, 4797
Bse21I CCTNAGG 2 cut(s) 1200, 4692
Bse3DI GCAATG 4 cut(s) 3278, 4923, 5057, 6774
Bse8I GATNNNNATC 5 cut(s) 2714, 2754, 3888, 4320, 6384
BseCI ATCGAT 1 cut(s) 7157
BseJI GATNNNNATC 5 cut(s) 2714, 2754, 3888, 4320, 6384
BseMI GCAATG 4 cut(s) 3278, 4923, 5057, 6774
BsePI GCGCGC 1 cut(s) 421
BseRI GAGGAG 5 cut(s) 191, 769, 800, 832, 941
BseSI GKGCMC 3 cut(s) 5305, 5623, 6349
BseYI CCCAGC 1 cut(s) 1187
BsgI GTGCAG 5 cut(s) 3555, 4176, 4567, 4656, 4779
Bsh1236I CGCG 9 cut(s) 38, 143, 423, 425, 892, 4062, 4861, 5988, 7150
Bsh1285I CGRYCG 2 cut(s) 219, 325
BshNI GGYRCC 2 cut(s) 598, 3877
BshVI ATCGAT 1 cut(s) 7157
BsiEI CGRYCG 2 cut(s) 219, 325
BsiHKCI CYCGRG 2 cut(s) 107, 5242
BsmBI CGTCTC 4 cut(s) 2786, 5786, 6074, 7514
BsmI GAATGC 5 cut(s) 3612, 5755, 6163, 7580, 7657
BsoBI CYCGRG 2 cut(s) 107, 5242
Bsp119I TTCGAA 1 cut(s) 414
Bsp1407I TGTACA 7 cut(s) 3822, 4264, 4396, 5800, 6234, 6399, 6906
Bsp1720I GCTNAGC 4 cut(s) 3684, 4200, 5726, 5991
Bsp19I CCATGG 4 cut(s) 1407, 4908, 5063, 5472
BspDI ATCGAT 1 cut(s) 7157
BspFNI CGCG 9 cut(s) 38, 143, 423, 425, 892, 4062, 4861, 5988, 7150
BspHI TCATGA 3 cut(s) 2022, 2718, 6445
BspLI GGNNCC 9 cut(s) 600, 778, 2650, 2857, 3879, 5376, 6727, 7065, 7448
BspMAI CTGCAG 6 cut(s) 1441, 1983, 2503, 3876, 4614, 4825
BspMI ACCTGC 2 cut(s) 5349, 6825
BspOI GCTAGC 1 cut(s) 1362
BspQI GCTCTTC 2 cut(s) 411, 2110
BspT104I TTCGAA 1 cut(s) 414
BspT107I GGYRCC 2 cut(s) 598, 3877
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 4 cut(s) 3278, 4923, 5057, 6774
BsrFI RCCGGY 4 cut(s) 153, 193, 3702, 4797
BsrGI TGTACA 7 cut(s) 3822, 4264, 4396, 5800, 6234, 6399, 6906
BssAI RCCGGY 4 cut(s) 153, 193, 3702, 4797
BssHII GCGCGC 1 cut(s) 421
BssNAI GTATAC 1 cut(s) 6843
BssNI GRCGYC 3 cut(s) 599, 1066, 5444
BssSI CACGAG 4 cut(s) 2580, 3443, 5323, 6204
BssT1I CCWWGG 9 cut(s) 1407, 2652, 3459, 3855, 4147, 4169, 4908, 5063, 5472
Bst1107I GTATAC 1 cut(s) 6843
Bst2BI CACGAG 4 cut(s) 2580, 3443, 5323, 6204
BstACI GRCGYC 3 cut(s) 599, 1066, 5444
BstAPI GCANNNNNTGC 6 cut(s) 2303, 5687, 5783, 6129, 6568, 7646
BstAUI TGTACA 7 cut(s) 3822, 4264, 4396, 5800, 6234, 6399, 6906
BstBAI YACGTR 3 cut(s) 2411, 4328, 4880
BstBI TTCGAA 1 cut(s) 414
BstDSI CCRYGG 4 cut(s) 1407, 4908, 5063, 5472
BstEII GGTNACC 1 cut(s) 3176
BstENI CCTNNNNNAGG 2 cut(s) 1840, 5345
BstFNI CGCG 9 cut(s) 38, 143, 423, 425, 892, 4062, 4861, 5988, 7150
BstH2I RGCGCY 2 cut(s) 602, 7501
BstHHI GCGC 8 cut(s) 145, 423, 425, 601, 1875, 5990, 7150, 7500
BstMCI CGRYCG 2 cut(s) 219, 325
BstPAI GACNNNNGTC 1 cut(s) 137
BstPI GGTNACC 1 cut(s) 3176
BstSLI GKGCMC 3 cut(s) 5305, 5623, 6349
BstSNI TACGTA 1 cut(s) 4328
BstUI CGCG 9 cut(s) 38, 143, 423, 425, 892, 4062, 4861, 5988, 7150
BstV2I GAAGAC 8 cut(s) 1163, 2207, 2281, 2348, 2560, 5027, 5307, 5522
BstXI CCANNNNNNTGG 1 cut(s) 5064
BstZ17I GTATAC 1 cut(s) 6843
Bsu15I ATCGAT 1 cut(s) 7157
Bsu36I CCTNAGG 2 cut(s) 1200, 4692
BsuI GTATCC 3 cut(s) 1309, 3945, 5492
BsuTUI ATCGAT 1 cut(s) 7157
BtgI CCRYGG 4 cut(s) 1407, 4908, 5063, 5472
BtgZI GCGATG 2 cut(s) 70, 2468
BtsI GCAGTG 4 cut(s) 481, 2390, 2484, 3838
BveI ACCTGC 2 cut(s) 5349, 6825
CaiI CAGNNNCTG 4 cut(s) 316, 1396, 4163, 4394
CciI TCATGA 3 cut(s) 2022, 2718, 6445
CfoI GCGC 8 cut(s) 145, 423, 425, 601, 1875, 5990, 7150, 7500
Cfr10I RCCGGY 4 cut(s) 153, 193, 3702, 4797
ClaI ATCGAT 1 cut(s) 7157
CseI GACGC 5 cut(s) 234, 2342, 5452, 5803, 5994
CsiI ACCWGGT 1 cut(s) 7449
CspCI CAANNNNNGTGG 2 cut(s) 1448, 1483
DinI GGCGCC 1 cut(s) 600
DraI TTTAAA 3 cut(s) 2904, 3157, 3942
DraIII CACNNNGTG 1 cut(s) 4545
DrdI GACNNNNNNGTC 1 cut(s) 5189
DseDI GACNNNNNNGTC 1 cut(s) 5189
EaeI YGGCCR 2 cut(s) 33, 151
EciI GGCGGA 1 cut(s) 6282
Ecl136II GAGCTC 4 cut(s) 112, 226, 2103, 5730
Eco105I TACGTA 1 cut(s) 4328
Eco130I CCWWGG 9 cut(s) 1407, 2652, 3459, 3855, 4147, 4169, 4908, 5063, 5472
Eco24I GRGCYC 6 cut(s) 100, 114, 228, 2105, 5377, 5732
Eco32I GATATC 2 cut(s) 1181, 2008
Eco53kI GAGCTC 4 cut(s) 112, 226, 2103, 5730
Eco81I CCTNAGG 2 cut(s) 1200, 4692
Eco88I CYCGRG 2 cut(s) 107, 5242
Eco91I GGTNACC 1 cut(s) 3176
EcoICRI GAGCTC 4 cut(s) 112, 226, 2103, 5730
EcoNI CCTNNNNNAGG 2 cut(s) 1840, 5345
EcoO109I RGGNCCY 1 cut(s) 2648
EcoO65I GGTNACC 1 cut(s) 3176
EcoRI GAATTC 5 cut(s) 1624, 2910, 3005, 7322, 7403
EcoRV GATATC 2 cut(s) 1181, 2008
EcoT14I CCWWGG 9 cut(s) 1407, 2652, 3459, 3855, 4147, 4169, 4908, 5063, 5472
EcoT22I ATGCAT 9 cut(s) 1869, 4506, 4596, 5755, 5899, 6163, 6385, 6610, 6614
EcoT38I GRGCYC 6 cut(s) 100, 114, 228, 2105, 5377, 5732
EgeI GGCGCC 1 cut(s) 600
EheI GGCGCC 1 cut(s) 600
ErhI CCWWGG 9 cut(s) 1407, 2652, 3459, 3855, 4147, 4169, 4908, 5063, 5472
Esp3I CGTCTC 4 cut(s) 2786, 5786, 6074, 7514
FauI CCCGC 2 cut(s) 35, 7143
FauNDI CATATG 5 cut(s) 1869, 2055, 3054, 4427, 4596
FbaI TGATCA 2 cut(s) 1216, 3916
FblI GTMKAC 2 cut(s) 215, 6842
FriOI GRGCYC 6 cut(s) 100, 114, 228, 2105, 5377, 5732
GlaI GCGC 8 cut(s) 144, 422, 424, 600, 1874, 5989, 7149, 7499
GsaI CCCAGC 1 cut(s) 1191
GsuI CTGGAG 4 cut(s) 113, 1654, 2937, 3558
HaeII RGCGCY 2 cut(s) 602, 7501
HgaI GACGC 5 cut(s) 234, 2342, 5452, 5803, 5994
HhaI GCGC 8 cut(s) 145, 423, 425, 601, 1875, 5990, 7150, 7500
Hin1I GRCGYC 3 cut(s) 599, 1066, 5444
Hin6I GCGC 8 cut(s) 143, 421, 423, 599, 1873, 5988, 7148, 7498
HinP1I GCGC 8 cut(s) 143, 421, 423, 599, 1873, 5988, 7148, 7498
HincII GTYRAC 5 cut(s) 216, 2223, 6538, 7394, 7524
HindII GTYRAC 5 cut(s) 216, 2223, 6538, 7394, 7524
HindIII AAGCTT 2 cut(s) 4854, 7073
Hpy99I CGWCG 4 cut(s) 143, 193, 224, 250
Hsp92I GRCGYC 3 cut(s) 599, 1066, 5444
HspAI GCGC 8 cut(s) 143, 421, 423, 599, 1873, 5988, 7148, 7498
KasI GGCGCC 1 cut(s) 598
KroI GCCGGC 2 cut(s) 193, 4797
KroNI GCCGGC 2 cut(s) 195, 4799
Ksp22I TGATCA 2 cut(s) 1216, 3916
LguI GCTCTTC 2 cut(s) 411, 2110
MabI ACCWGGT 1 cut(s) 7449
MbiI CCGCTC 1 cut(s) 70
MfeI CAATTG 6 cut(s) 1815, 2301, 3387, 5738, 6962, 7428
Mly113I GGCGCC 1 cut(s) 599
MlyI GAGTC 4 cut(s) 572, 4091, 4172, 6065
MmeI TCCRAC 8 cut(s) 414, 498, 668, 2265, 3247, 3903, 3959, 6253
Mph1103I ATGCAT 9 cut(s) 1869, 4506, 4596, 5755, 5899, 6163, 6385, 6610, 6614
MroNI GCCGGC 2 cut(s) 193, 4797
MroXI GAANNNNTTC 6 cut(s) 350, 1170, 4990, 5431, 5529, 5592
MspA1I CMGCKG 9 cut(s) 1892, 3509, 4551, 4656, 4736, 5147, 6311, 6848, 7337
MunI CAATTG 6 cut(s) 1815, 2301, 3387, 5738, 6962, 7428
Mva1269I GAATGC 5 cut(s) 3612, 5755, 6163, 7580, 7657
MvnI CGCG 9 cut(s) 38, 143, 423, 425, 892, 4062, 4861, 5988, 7150
NaeI GCCGGC 2 cut(s) 195, 4799
NarI GGCGCC 1 cut(s) 599
NciI CCSGG 5 cut(s) 74, 326, 546, 2097, 3720
NcoI CCATGG 4 cut(s) 1407, 4908, 5063, 5472
NdeI CATATG 5 cut(s) 1869, 2055, 3054, 4427, 4596
NgoMIV GCCGGC 2 cut(s) 193, 4797
NheI GCTAGC 1 cut(s) 1358
NlaIV GGNNCC 9 cut(s) 600, 778, 2650, 2857, 3879, 5376, 6727, 7065, 7448
NmeAIII GCCGAG 2 cut(s) 316, 4990
NmuCI GTSAC 7 cut(s) 1007, 1479, 1937, 1999, 2965, 3176, 7078
NsiI ATGCAT 9 cut(s) 1869, 4506, 4596, 5755, 5899, 6163, 6385, 6610, 6614
NspV TTCGAA 1 cut(s) 414
PaeI GCATGC 4 cut(s) 1810, 2446, 6612, 6616
PagI TCATGA 3 cut(s) 2022, 2718, 6445
PauI GCGCGC 1 cut(s) 421
PciI ACATGT 6 cut(s) 1084, 1263, 1294, 2147, 7518, 7698
PciSI GCTCTTC 2 cut(s) 411, 2110
PcsI WCGNNNNNNNCGW 4 cut(s) 60, 1710, 4324, 6427
PctI GAATGC 5 cut(s) 3612, 5755, 6163, 7580, 7657
PdiI GCCGGC 2 cut(s) 195, 4799
PdmI GAANNNNTTC 6 cut(s) 350, 1170, 4990, 5431, 5529, 5592
PflFI GACNNNGTC 1 cut(s) 7451
PflMI CCANNNNNTGG 4 cut(s) 1413, 2865, 3593, 7456
PfoI TCCNGGA 1 cut(s) 2833
PleI GAGTC 4 cut(s) 571, 4090, 4172, 6064
PluTI GGCGCC 1 cut(s) 602
PpsI GAGTC 4 cut(s) 571, 4090, 4172, 6064
Ppu21I YACGTR 3 cut(s) 2411, 4328, 4880
PpuMI RGGWCCY 1 cut(s) 2648
PscI ACATGT 6 cut(s) 1084, 1263, 1294, 2147, 7518, 7698
PshAI GACNNNNGTC 1 cut(s) 137
PshBI ATTAAT 2 cut(s) 1454, 7470
PsiI TTATAA 2 cut(s) 4631, 6600
Psp124BI GAGCTC 4 cut(s) 114, 228, 2105, 5732
Psp1406I AACGTT 2 cut(s) 165, 2801
Psp5II RGGWCCY 1 cut(s) 2648
PspEI GGTNACC 1 cut(s) 3176
PspFI CCCAGC 1 cut(s) 1187
PspN4I GGNNCC 9 cut(s) 600, 778, 2650, 2857, 3879, 5376, 6727, 7065, 7448
PspPPI RGGWCCY 1 cut(s) 2648
PstI CTGCAG 6 cut(s) 1441, 1983, 2503, 3876, 4614, 4825
PstNI CAGNNNCTG 4 cut(s) 316, 1396, 4163, 4394
PsyI GACNNNGTC 1 cut(s) 7451
PteI GCGCGC 1 cut(s) 421
PvuII CAGCTG 8 cut(s) 1892, 3509, 4551, 4656, 5147, 6311, 6848, 7337
SacI GAGCTC 4 cut(s) 114, 228, 2105, 5732
SalI GTCGAC 1 cut(s) 214
SapI GCTCTTC 2 cut(s) 411, 2110
SchI GAGTC 4 cut(s) 572, 4091, 4172, 6065
SexAI ACCWGGT 1 cut(s) 7449
SfoI GGCGCC 1 cut(s) 600
SfuI TTCGAA 1 cut(s) 414
SmlI CTYRAG 6 cut(s) 962, 2808, 3847, 4493, 5111, 6263
SmoI CTYRAG 6 cut(s) 962, 2808, 3847, 4493, 5111, 6263
SnaBI TACGTA 1 cut(s) 4328
SphI GCATGC 4 cut(s) 1810, 2446, 6612, 6616
SspDI GGCGCC 1 cut(s) 598
SspI AATATT 2 cut(s) 1366, 2202
SstI GAGCTC 4 cut(s) 114, 228, 2105, 5732
StyI CCWWGG 9 cut(s) 1407, 2652, 3459, 3855, 4147, 4169, 4908, 5063, 5472
TaqII GACCGA 1 cut(s) 4374
TauI GCSGC 7 cut(s) 38, 70, 2338, 2417, 3085, 5402, 6854
TseFI GTSAC 7 cut(s) 1007, 1479, 1937, 1999, 2965, 3176, 7078
Tsp45I GTSAC 7 cut(s) 1007, 1479, 1937, 1999, 2965, 3176, 7078
TspGWI ACGGA 4 cut(s) 177, 2208, 3225, 6062
Tth111I GACNNNGTC 1 cut(s) 7451
Van91I CCANNNNNTGG 4 cut(s) 1413, 2865, 3593, 7456
VneI GTGCAC 2 cut(s) 5301, 6345
VspI ATTAAT 2 cut(s) 1454, 7470
XagI CCTNNNNNAGG 2 cut(s) 1840, 5345
XbaI TCTAGA 2 cut(s) 1882, 6034
XcmI CCANNNNNNNNNTGG 1 cut(s) 6941
XmiI GTMKAC 2 cut(s) 215, 6842
XmnI GAANNNNTTC 6 cut(s) 350, 1170, 4990, 5431, 5529, 5592
ZraI GACGTC 1 cut(s) 1067
Zsp2I ATGCAT 9 cut(s) 1869, 4506, 4596, 5755, 5899, 6163, 6385, 6610, 6614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.