Rroxscaffold_7G00156680

phosphatidylinositol-3-phosphate binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005768
Physical Location & Seq
Reverse (-)
593590 .. 615104
21515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00156680.1

Sequence Viewer

Length: 7050 bp
ATGGAGGACAAAGAAACCGAAATCCTTTCCCGCCTGGCCGCAAACCACCTCTTCCTCGGCCAATTCGAGCCCCTCCGAGCCATCCTCATCGCTCTCCGCGCCCGAAACCCCGCCCTCGCCCTCGCCCTCCTCCAGACCATAGTCGCCCACTCCGCTCGCTTCGACAATGTTCTATGGTCCCCGTCCTGCCCTTCCCCGGCGATCCTCACTTACCTCTCCACCGTCGAGCTCTTGCAATTCGACGACGCCTCCTCCGCCTGGGGATTCGATCCCGAAACGCTGCGGCTGCGCGCCGAGTTTCTGCTGCTGGTTCAGAGCTTGATCGATAGGGTTTCGGAGAGCCTGCGGAGGAGTTTCGATTTGGAGAGCATAGAGAAGGAGAAGGAGAGGAGTGATGGTTTCGAAGAGCGGGCGGAGTTGATGGATGATACGATGAGCAAGGAGTTGAGAGACGGCGGTGGTGAATTGGAGACCTGTGTTAGGGTTTTGGATAGAGTATTGGAGTTGGGGGTGAGCAGGTTGAAGCCGGAATCGTTACTAGTAGGGAATGGTGATCAGGCTGTGGAAATTGAGGAAGGCGAGTTCATGTGCTTGAGGAGTGTGGTGTGGGAGTATGCTGATGTTTTCGATGCCTTGTGTTGGAATGTACAGAGGCAAGTGAGGGGTTGGGAGGGAGATGATGCTTCGGGTTTGGCCATAACAGTGAGGAGGGATGATATGCCGAAAGAGGAGGATGTGAAGGTTCTTCGTTTGATACAGAGGAGTGTACAGTTAGCTCACTTGGATGCTATGAGAGAGTGCATCAAGGATGGTCAAGTGGATGGAGTGGTTTCGCGGATTCGGTTTCTTCACCTTGATTATGGAGTGGAGGAGACTGAGTATCGCATTGCTTTGCAAGATCTCCTCAAGATGGTGTCATCAGGAAAGGAGGGATATGGAGATTCCTGGCGAGACATGAGAGAAATATTACTGCAGATTTATAGTGCAGCTCTTGCTTCAAACTCTGGGCATCTTGTTCAAATGATACAAGTTCTTCAGGATGAGTTACTCTCAAAAGAGATTGAGATATATAGATCTCTTGATAACAATCAAATTCCGCCACCTCTTGAACGTCTTCAAACATATCTTGAAGAATTAAAACCTGGCACAGATGTAAATGATAAAACTTCTCCCTTCAATAGTGTAGTTGCATTTTGCATGAGAGACATGTACCATTATGCTCGTGTTTCTGGTTTACACCTGCTTGAATGTGTCATGGAGACAGCTGTGTCTGTGGTGAAGAGGGAGCAGCTTCAAGAAGCTAGCAATATTCTTTTATTGTTCCCCCGTCTTCAGCCCCTAGTAGCTGCCATGGGTTGGGATTTACTGTCTGGCAAAACTGCAGCACGAAGGAAATTAATGCAGCTGCTGTGGAAAACTAAGTCGCAGGTTTTACGGCTGGAAGAATCTTCCCTTTATAGTAACCAGTCAGATGAGATATCTTGCGTGGAATATCTTTGTGATTCTTTATGTTATCAACTTGATCTTGCCTCATTTGTGGCCTGTGTTAATTCTGGTCAATCATGGAATTCAAAATTATCACTGACATTGTCTGCAAAAGACCAAATAGCATTCAATGGTGAAGATGCCCAGTTAGACCCTTTTGTTGAAAACTTCGTACTGGAAAGGCTTTCCGCTCAAAGTCCTCTTCGGGTACTGTTTGATGTTGTTCCAGGCATAAAATTTCAGGATGCTATTGAATTGATAAGCATGCAGCCAATTGCTTCAACTTTAGAAGCCTGGAAGAGGATGCAGGATATTGAACTTATGCACATGCGGTATGCTCTAGATTCAGCCGTTCTTGCACTTGGAGTGATGGAAAAGAGTATGACCGCTGAAAGTCATCAAGTTGCATTTTGCTATTTAAAAGACCTGCAGAACCATTTGGAGGCTGTAAATACTATCCCCCGCAAGATCATGATCGTGAATGTCATAATTTCGCTCTTGCATATGGATGATCAGTCTCTCAATTTGAATCATTGTGCCTCACCAGGGAGTTATTCTGAAGCACACTACACATGTGCTTCAGAACAGATCAATCTGACTACATGTGAAGGGGGAAATGAATTGGTTATATCTTTCACAGGCAAACTACTTGAGATATTACATCACTGTCTTCCTTCAACGATAGCGGACCGTGATCATGCTCTTAGTGATGGTATAAATAGGGGTGGAAGACAAGCAGTGGAGTGGAGAGTGTCAATTGCTAAACACTTTATTGAAGAGTGGGAATGGCGGTTGTCAATTTTGCAGCGTCTCCTTCCATTATCTGAACGTCAGTGGAAATGGAAGGAAGCATTGACGGTATTACGTGCTGCACCATCTAAGCTACTTAACCTAGCTACACTTGAATTAGCTGAATGGGTAGATAGTGCTGTCAGAAGACAGTCGGTAGAAGATGTAGTCTCTCGTGCTGCTGATGATGGAACTTCTACAGTACATGATCTGGATTTTTCATCATTACGCTCTCAATTAGGTCCTTTGGCTGCAGCTCAAGTTATGCTGTCTGAGATATATCCAGGAGTTTCTCCAAAGATGGGCTCCACGTACTGGGATCAGATTCTTGAAGTGGGAGTTATTTCTGTTCTAAAAAGAATCCTCAAGCGTTTACATGAGTTCTTGGATCAGGATGATCCTCCAGCCCTTCAGGCCACTCTGTCTGGGGAGATTTTCATCTCCTCGCCAAAGGACTCCCATCGACTGGGACAACGGGAACGTAAGCTCCATAATCTTGCAAGAGCTGTGGCTGATGAAGAATCAGAGCTCAATTTTTCTAAAGGAGAAGGCCCTTCTGTTGACCAAAAGGTTCTATCCGATTATGACAAGGATGGAGTTCTTGGACTGGGGTTAAGAGTTGCCAAACAGATACCTTCAAGTTCCACAATTGGAGAAACTAGTGTGCAGCCTGTTGTTTATGATGTAAAGGACAGTGGGAAGAGATTGTTTGGTCCATTAAGTACCAAACCCATGACATATCTTTCACAATTTATTCTTCACATTGCTGCAATTGGTGACATAGTTGATGGCACAGATACAACTCATGATTTCAACTTTTTCTCCCTGGTGTATGAGTGGCCCAAGGATCTTTTAACCCGTCTTGTCTTTGACCGGGGCAGCACTGATGCAGCTGGTAAGGTAGCTGAGATTATGTGTGCAGATTTTGTGCATGAATTAGATATAGTAAAACATTTGGTCAAACTATCTCCAGTGAGGGCAGTCCTGGCATGTGTATTTGGGAGTAGCATTTTGTACAATGGCAGTAATTCTTCTATATCGAGCTCCTTGGATGATGAACTTCTACAGGCACCAGATGTTGACCGGTTGTTTTATGAGTTTGCTCTTGATCAATCTGAGAGGTTCCCAACTTTAAACCGATGGATACAAATGCAAACTAACCTGCATCGAGTTTCAGAGTTTGCTGTAATGGTTAAGCAAACTGATGATGGTGGTGAGACTAGAGGTGAAGCAAGAGCTGCTATCAAGAGGCTTCGTGAGCTTGACAGTGATACTGAGTCCGAAGTTGATGACGTTGTTGGTAATAGTGTTTCAACAGCTTTACCAGACCTCGATAGCCAAGGTGGTACTGCTCTTGATTCATGGCGTGATTCTTCTGAACCAGATGTTGCTGAGTTTGACACTTCAGTTTTCCTTTCTTTTGATTGGGAAAATGAAGAACCTTATGAGAAAGCTGTACAAAGGACTGGCATTGCTAATGACTTTAAAAGGATGACCTACTACAACATCTACTCAGAGATATTCCAATTGATAGATGATGAAAAACTTATGGATGCTCTTGCTCTTTCCGATCGCTTTCTACGCAATGGAGCTTCAGATCAACTACTTCAGCTACTCATTGAACATGGGGAAGAGAACCAGTCAGTCTCTGGACACTCTCAGGGTTACGGTGGCAATAGCATTTGGAGCACTAGTTGGCAGTATTGCCTGCGGTTGAAGGATAAACAAGTGGCTGCCAGACTTGCTCTCAAGTGTATGCATAGATGGGAACTGGATGCTGCTTTAGACGTACTCACCATGTGCAGCTGCCACCTGCCTCAAAGTGACCCCATTAGGGAGGAGGTCATGTATAGGAGACAAGCTTTGCAGAGGTTGAGGCAGAGTGTAAGGAAGATCCTGAGGGGGTTGGCACTTAGATTAGCTGGAAAAGGAGCTGTTTCTGCTGCCTTAGAAGTGGCTGAAAGCGCGGGGTTATCCATTGATTTGCGTAGGGAACTGCAGGGCCGGCAACTTGTGAAGCTTTTGACTGCAGATCCTCTCAATGGGGGTGGTCCAGCAGAAGCTTCACGATTTCTTTCTTCATTGCGCGACTCAGATGATGCTCTACCAGTTGCCATGGGTGCGATGCAGCTTTTACCTGATTTGCGGTCAAAGCAGCTTCTTGTTCATTTCTTCCTGAAGCGGAGAGAAGGGAATCTTTCAGATGTTGAGGTTTCTCGGCTTAATTCATGGGCCTTGGGGCTTCGTGTTCTGGCTGCCTTGCCATTGCCATGGCAGCAAAGATGTTCTTCCTTGCATGAGCACCCTCATTTGATACTTGAGGTTCTTCTGATGAGGAAACAACTACAATCTGCTGCTTTGATTCTCAAAGAATTTCCTTTGTTGAGAGACAATAATGCGCTTATTGCCTATGCTACTAAAGCTATTGCAATCAGTATTAGCTCTCCCCCTAGAGAACATCGAGTATCTGTTTCGGGGACAAGATTAAAGCAGAAAACAAGAACAGGTGCACCTGTGAAGTCTTCTTTCACTAGCAGTCTCAGTAACTTGCAAAAAGAAGCTCGCAGAGCCTTCTCTTGGGCTCCACGAAATAGTGGAGACAGGTCCACCCCCAAAGATGGTTACCGTAAGAGAAAGAGTTCTGGATTGACACCCTCTGAAAAAGTAGCTTGGGAGGCCATGGCTGGAATCCAGGAGGATCGTGCTTCAACATATTCTGTTGATGGGCAAGAACGGCTTCCTTCAATTTCAATATCTGAAGAATGGATGCTAACTGGTGATCCATTGAAGGATGAAGCCGTTCGTGCATCTCACCGATACGAGAGTGCTCCTGACATAACGCTTTTCAAGCATGCCAAGTTCTTGTCTAGGCATAACGCGTCTGTTGCTGAGAGTTCAGGCTTCTCTGTGCAGGCACTGCTTTCTCTATGCTCAGATGATTCTGTGTCTGCCAAAACTGCATTGGATTTGTGCGTAACTCAGATGAAGAATGTACTGAGTTCCCAACAATTGCCAGAGAATGCATCTGTGGAAACCATTGGCCGAGCATATCATGCCACAGAGACGTTTGTACAGGGGCTACTTTATGCTAAATCACTGTTGAGAAAGCTTGTGGGCAGCAGTGATTTGTTGAGTAATTCTGAAAGAAGTAGGGATGCCGATGATGCATCTTCTGATGCTGGTAGCTCTAGTGTGGGTAGTCAGTCCACAGATGAGCTATCTGAAGTTCTGTTGCAGGCAGACACTTGGCTAGGACGTGCTGAGCTGCTTCAGAGCCTTCTGGGATCAGGAATTGCTGCTTCTCTAGATGATATTGCGGATAAAGAGTCATCTGCCTCTCTTCGTGATAGGCTGATTGTGGAAGAACGGTACAGCATGGCCGTATATACATGTAAAAAGTGCAAGATTGATGTGGTTCCTGTTTGGAATGCATGGGGACATGCTTTGATTCGGATGGAGCACTATGCACAAGCTCGCGTGAAGTTCAAGCAAGCTCTTCAGCTGTACAAAGGTGATCCTGCTCCTGTTATTCTTGAGATAATCAATACAATTGAAGGAGGTCCGCCAGTTGATGTTTCAGCTGTTCGTTCCATGTATGAACATTTGGCTAAAAGTGCACCAACAATCTTGGATGATTCTCTTTCTGCTGATTCATATCTCAATGTGTTATACATGCCATCCACATTTCCTCGTTCAGAGAGATCCAGGCGTTCTCAGGAATCTGCAAATAACAGCTCCGCATATATGTCAGACTTTGAAGATGGACCACGCAGCAACTTAGACAGTGTTCGGTATGTTGAGTGTGTCAACTATTTACAAGAATATGCCAGGCAGCATTTGCTCAATTTCATGTTCAGGCATGGCCACTATAATGACGCTTGCGTGTTGTTCTTTCCTCAAAATGCTGTTCCCCCTCCTCCTCAGCCATCAGTAGCAGGGGTGGCATCCTCATCTTCATCCCCTCAAAGACCAGATCCTCTAGGAACTGATTATGGGACCATTGATGATTTGTGTGATTTATGTGTTGGTTATGGCGCAATGCATGTTTTAGAAGAAGTGATCTCAGTAAGAATGTCATCTACAACCCCACAAGATGTGGCAGTGATCCAGCATACAGCTGCAGCCCTTGCTCGTATTTGTGTCTACTGTGAAACACATAGGCATTTCAATTATTTATACAAGTTTCAGGTTATTAAAAAGGACCATGTCGCTGCTGGACTGTGCTGCATCCAACTGTTTATGAACTCTTCTTTGCAAGAGGAAGCTATAAAACATTTGGAAAATTCCAAGATGCACTTTGATGAAGCGCTATCAGCACGGTACAAAGGTGGGGATTCTACTAAGCTTGTGACGAAGGGTGCTAGAGGAAAAAGTGCCTCTGAAAAGCTCACTGAAGAAGGACTTGTCAAGTTTTCTGCTCGGTTATCAATCCAGGTTGAAGTTGTGAGATCCTATAATGATTCAGATGGACCCCATTGGAAACATTCACTTTTCGGAAACCCAAATGACTCCGAAACATTCAGGAGAAGGTGTAAAATTGCTGAATCTCTTGTGGAAAAGAACTTCGATTTGGCATTCCAAGTGATTTATGAATTCACCCTTCCAGCTGTGGATATATATGCTGGTGTAGCTGCATCACTTGCTGAGAGAAAAAAAGGTAGTCAGCTGACAGAATTTTTTAGAAACATTAAAGGGACCATTGATGACGATGATTGGGACCAGGTCTTGGGAGCTGCAATTAATGTATATGCTAACAAACACAAAGAACGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2349

Amino Acids

262.36

Weight (kDa)

5.34

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ZFYVE26 PF25569 2293 - 2347 4e-07 ZFYVE26-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1248, 4095
AasI GACNNNNNNGTC 1 cut(s) 1267
Acc36I ACCTGC 6 cut(s) 507, 1248, 1417, 1920, 3444, 4095
AccB1I GGYRCC 1 cut(s) 3343
AccB7I CCANNNNNTGG 4 cut(s) 1356, 2589, 2740, 7000
AccBSI CCGCTC 3 cut(s) 155, 409, 1676
AccI GTMKAC 1 cut(s) 6417
AccII CGCG 7 cut(s) 99, 291, 835, 4241, 4362, 5153, 5751
AcoI YGGCCR 6 cut(s) 36, 58, 693, 5314, 5652, 6136
AcsI RAATTY 7 cut(s) 1092, 1567, 1721, 4646, 6556, 6866, 6947
AcyI GRCGYC 1 cut(s) 246
AdeI CACNNNGTG 1 cut(s) 4074
AfeI AGCGCT 1 cut(s) 6582
AflIII ACRYGT 5 cut(s) 1206, 2057, 2087, 5151, 5663
AgeI ACCGGT 1 cut(s) 3357
AhlI ACTAGT 3 cut(s) 538, 2933, 3965
AjiI CACGTC 1 cut(s) 5531
AjuI GAANNNNNNNTTGG 2 cut(s) 6827, 6859
AloI GAACNNNNNNTCC 4 cut(s) 566, 598, 2745, 2777
Alw21I GWGCWC 9 cut(s) 231, 2805, 3320, 3965, 4578, 4786, 5104, 5736, 5893
Alw44I GTGCAC 2 cut(s) 4782, 5889
AlwNI CAGNNNCTG 3 cut(s) 1408, 3923, 5191
Aor51HI AGCGCT 1 cut(s) 6582
ApaLI GTGCAC 2 cut(s) 4782, 5889
ApoI RAATTY 7 cut(s) 1092, 1567, 1721, 4646, 6556, 6866, 6947
AseI ATTAAT 2 cut(s) 1397, 7014
AsiGI ACCGGT 1 cut(s) 3357
Asp700I GAANNNNTTC 4 cut(s) 1113, 4912, 5010, 5073
AspLEI GCGC 8 cut(s) 101, 291, 293, 4241, 4362, 4675, 6311, 6583
AsuC2I CCSGG 2 cut(s) 197, 3149
AsuII TTCGAA 1 cut(s) 402
AsuNHI GCTAGC 1 cut(s) 1301
AxyI CCTNAGG 1 cut(s) 4172
BaeGI GKGCMC 2 cut(s) 4786, 5893
BaeI ACNNNNGTAYC 4 cut(s) 5083, 5116, 6586, 6619
BalI TGGCCA 2 cut(s) 695, 6138
BanI GGYRCC 1 cut(s) 3343
BanII GRGCYC 6 cut(s) 72, 231, 2582, 2805, 3320, 4858
BauI CACGAG 2 cut(s) 1221, 2448
BbsI GAAGAC 6 cut(s) 1106, 1322, 2147, 2221, 2428, 4788
Bbv12I GWGCWC 9 cut(s) 231, 2805, 3320, 3965, 4578, 4786, 5104, 5736, 5893
BbvCI CCTCAGC 1 cut(s) 6195
BceAI ACGGC 6 cut(s) 469, 1451, 1820, 5024, 5057, 5639
BciVI GTATCC 1 cut(s) 3411
BclI TGATCA 4 cut(s) 553, 1996, 2179, 3382
BcnI CCSGG 2 cut(s) 197, 3149
BcuI ACTAGT 3 cut(s) 538, 2933, 3965
BfmI CTRYAG 9 cut(s) 971, 1380, 1913, 2472, 2526, 3338, 4271, 4302, 6393
BfoI RGCGCY 1 cut(s) 6584
BfuAI ACCTGC 6 cut(s) 507, 1248, 1417, 1920, 3444, 4095
BfuI GTATCC 1 cut(s) 3411
BglI GCCNNNNNGGC 1 cut(s) 2687
BglII AGATCT 2 cut(s) 898, 1073
BlpI GCTNAGC 1 cut(s) 5535
BmgBI CACGTC 1 cut(s) 5531
BmrI ACTGGG 4 cut(s) 1624, 2599, 2750, 2891
BmtI GCTAGC 1 cut(s) 1305
BmuI ACTGGG 4 cut(s) 1624, 2599, 2750, 2891
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 6 cut(s) 1106, 1322, 2147, 2221, 2428, 4788
BplI GAGNNNNNCTC 4 cut(s) 69, 101, 1034, 1066
BpmI CTGGAG 3 cut(s) 116, 2661, 3228
Bpu10I CCTNAGC 1 cut(s) 6195
Bpu1102I GCTNAGC 1 cut(s) 5535
Bpu14I TTCGAA 1 cut(s) 402
BpuEI CTTGAG 8 cut(s) 613, 890, 2156, 2517, 2624, 4007, 4613, 5828
BpuMI CCSGG 2 cut(s) 197, 3149
Bsa29I ATCGAT 1 cut(s) 324
BsaAI YACGTR 2 cut(s) 2351, 2586
BsaBI GATNNNNATC 4 cut(s) 1086, 1958, 2711, 5928
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 464
BsaWI WCCGGW 1 cut(s) 3357
Bse118I RCCGGY 2 cut(s) 3357, 4278
Bse21I CCTNAGG 1 cut(s) 4172
Bse3DI GCAATG 7 cut(s) 885, 3036, 3744, 3865, 4355, 4538, 6318
Bse8I GATNNNNATC 4 cut(s) 1086, 1958, 2711, 5928
BseCI ATCGAT 1 cut(s) 324
BseJI GATNNNNATC 4 cut(s) 1086, 1958, 2711, 5928
BseMI GCAATG 7 cut(s) 885, 3036, 3744, 3865, 4355, 4538, 6318
BsePI GCGCGC 1 cut(s) 289
BseSI GKGCMC 2 cut(s) 4786, 5893
BsgI GTGCAG 6 cut(s) 1005, 2340, 2960, 3213, 4096, 5204
Bsh1236I CGCG 7 cut(s) 99, 291, 835, 4241, 4362, 5153, 5751
Bsh1285I CGRYCG 1 cut(s) 3847
BshNI GGYRCC 1 cut(s) 3343
BshTI ACCGGT 1 cut(s) 3357
BshVI ATCGAT 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 3847
BsiHKAI GWGCWC 9 cut(s) 231, 2805, 3320, 3965, 4578, 4786, 5104, 5736, 5893
BsiSI CCGG 5 cut(s) 197, 527, 3148, 3358, 4279
BslFI GGGAC 7 cut(s) 163, 2757, 4765, 5724, 6283, 6982, 7004
BsmBI CGTCTC 3 cut(s) 444, 2300, 5330
BsmFI GGGAC 7 cut(s) 163, 2757, 4765, 5724, 6283, 6982, 7004
BsmI GAATGC 4 cut(s) 1610, 5299, 5707, 6848
Bso31I GGTCTC 1 cut(s) 464
Bsp119I TTCGAA 1 cut(s) 402
Bsp1407I TGTACA 6 cut(s) 646, 766, 3288, 3730, 5344, 5778
Bsp1720I GCTNAGC 1 cut(s) 5535
Bsp19I CCATGG 4 cut(s) 1350, 4389, 4544, 4953
BspDI ATCGAT 1 cut(s) 324
BspFNI CGCG 7 cut(s) 99, 291, 835, 4241, 4362, 5153, 5751
BspHI TCATGA 2 cut(s) 1956, 3079
BspMAI CTGCAG 7 cut(s) 975, 1384, 1917, 2530, 4275, 4306, 6397
BspMI ACCTGC 6 cut(s) 507, 1248, 1417, 1920, 3444, 4095
BspOI GCTAGC 1 cut(s) 1305
BspQI GCTCTTC 2 cut(s) 399, 5775
BspT104I TTCGAA 1 cut(s) 402
BspT107I GGYRCC 1 cut(s) 3343
BspTNI GGTCTC 1 cut(s) 464
BsrBI CCGCTC 3 cut(s) 155, 409, 1676
BsrDI GCAATG 7 cut(s) 885, 3036, 3744, 3865, 4355, 4538, 6318
BsrFI RCCGGY 2 cut(s) 3357, 4278
BsrGI TGTACA 6 cut(s) 646, 766, 3288, 3730, 5344, 5778
BssAI RCCGGY 2 cut(s) 3357, 4278
BssHII GCGCGC 1 cut(s) 289
BssNI GRCGYC 1 cut(s) 246
BssSI CACGAG 2 cut(s) 1221, 2448
BssT1I CCWWGG 8 cut(s) 1350, 3117, 3321, 3613, 4389, 4509, 4544, 4953
Bst2BI CACGAG 2 cut(s) 1221, 2448
BstACI GRCGYC 1 cut(s) 246
BstAPI GCANNNNNTGC 7 cut(s) 992, 3512, 5191, 5327, 6112, 6401, 6914
BstAUI TGTACA 6 cut(s) 646, 766, 3288, 3730, 5344, 5778
BstBAI YACGTR 2 cut(s) 2351, 2586
BstBI TTCGAA 1 cut(s) 402
BstDSI CCRYGG 4 cut(s) 1350, 4389, 4544, 4953
BstEII GGTNACC 1 cut(s) 4895
BstENI CCTNNNNNAGG 2 cut(s) 478, 1783
BstFNI CGCG 7 cut(s) 99, 291, 835, 4241, 4362, 5153, 5751
BstH2I RGCGCY 1 cut(s) 6584
BstHHI GCGC 8 cut(s) 101, 291, 293, 4241, 4362, 4675, 6311, 6583
BstMCI CGRYCG 1 cut(s) 3847
BstPAI GACNNNNGTC 1 cut(s) 140
BstPI GGTNACC 1 cut(s) 4895
BstSFI CTRYAG 9 cut(s) 971, 1380, 1913, 2472, 2526, 3338, 4271, 4302, 6393
BstSLI GKGCMC 2 cut(s) 4786, 5893
BstUI CGCG 7 cut(s) 99, 291, 835, 4241, 4362, 5153, 5751
BstV2I GAAGAC 6 cut(s) 1106, 1322, 2147, 2221, 2428, 4788
BstX2I RGATCY 8 cut(s) 898, 1073, 3121, 4167, 4306, 5975, 6247, 6722
BstXI CCANNNNNNTGG 1 cut(s) 4545
BstYI RGATCY 8 cut(s) 898, 1073, 3121, 4167, 4306, 5975, 6247, 6722
Bsu15I ATCGAT 1 cut(s) 324
Bsu36I CCTNAGG 1 cut(s) 4172
BsuI GTATCC 1 cut(s) 3411
BsuTUI ATCGAT 1 cut(s) 324
BtgI CCRYGG 4 cut(s) 1350, 4389, 4544, 4953
BtgZI GCGATG 2 cut(s) 73, 4412
BtrI CACGTC 1 cut(s) 5531
BtsI GCAGTG 4 cut(s) 2229, 5189, 5401, 6381
BveI ACCTGC 6 cut(s) 507, 1248, 1417, 1920, 3444, 4095
CaiI CAGNNNCTG 3 cut(s) 1408, 3923, 5191
CciI TCATGA 2 cut(s) 1956, 3079
CfoI GCGC 8 cut(s) 101, 291, 293, 4241, 4362, 4675, 6311, 6583
Cfr10I RCCGGY 2 cut(s) 3357, 4278
ClaI ATCGAT 1 cut(s) 324
CpoI CGGWCCG 1 cut(s) 2173
CseI GACGC 4 cut(s) 254, 2282, 5142, 6158
CsiI ACCWGGT 1 cut(s) 6993
CspAI ACCGGT 1 cut(s) 3357
CspCI CAANNNNNGTGG 4 cut(s) 2763, 2798, 4303, 4338
CspI CGGWCCG 1 cut(s) 2173
DraI TTTAAA 3 cut(s) 1905, 3408, 3760
DraIII CACNNNGTG 1 cut(s) 4074
DrdI GACNNNNNNGTC 1 cut(s) 1267
DseDI GACNNNNNNGTC 1 cut(s) 1267
EaeI YGGCCR 6 cut(s) 36, 58, 693, 5314, 5652, 6136
EciI GGCGGA 4 cut(s) 244, 428, 1086, 5826
Ecl136II GAGCTC 3 cut(s) 229, 2803, 3318
Eco130I CCWWGG 8 cut(s) 1350, 3117, 3321, 3613, 4389, 4509, 4544, 4953
Eco24I GRGCYC 6 cut(s) 72, 231, 2582, 2805, 3320, 4858
Eco31I GGTCTC 1 cut(s) 464
Eco32I GATATC 1 cut(s) 1479
Eco47III AGCGCT 1 cut(s) 6582
Eco53kI GAGCTC 3 cut(s) 229, 2803, 3318
Eco81I CCTNAGG 1 cut(s) 4172
Eco91I GGTNACC 1 cut(s) 4895
EcoICRI GAGCTC 3 cut(s) 229, 2803, 3318
EcoNI CCTNNNNNAGG 2 cut(s) 478, 1783
EcoO109I RGGNCCY 2 cut(s) 2516, 2825
EcoO65I GGTNACC 1 cut(s) 4895
EcoRI GAATTC 2 cut(s) 1567, 6866
EcoRV GATATC 1 cut(s) 1479
EcoT14I CCWWGG 8 cut(s) 1350, 3117, 3321, 3613, 4389, 4509, 4544, 4953
EcoT22I ATGCAT 5 cut(s) 4035, 5299, 5443, 5707, 6318
EcoT38I GRGCYC 6 cut(s) 72, 231, 2582, 2805, 3320, 4858
ErhI CCWWGG 8 cut(s) 1350, 3117, 3321, 3613, 4389, 4509, 4544, 4953
Esp3I CGTCTC 3 cut(s) 444, 2300, 5330
FaqI GGGAC 7 cut(s) 163, 2757, 4765, 5724, 6283, 6982, 7004
FauI CCCGC 5 cut(s) 38, 118, 402, 1955, 4234
FauNDI CATATG 1 cut(s) 1989
FbaI TGATCA 4 cut(s) 553, 1996, 2179, 3382
FblI GTMKAC 1 cut(s) 6417
FriOI GRGCYC 6 cut(s) 72, 231, 2582, 2805, 3320, 4858
GlaI GCGC 8 cut(s) 100, 290, 292, 4240, 4361, 4674, 6310, 6582
GsuI CTGGAG 3 cut(s) 116, 2661, 3228
HaeII RGCGCY 1 cut(s) 6584
HapII CCGG 5 cut(s) 197, 527, 3148, 3358, 4279
HgaI GACGC 4 cut(s) 254, 2282, 5142, 6158
HhaI GCGC 8 cut(s) 101, 291, 293, 4241, 4362, 4675, 6311, 6583
Hin1I GRCGYC 1 cut(s) 246
Hin6I GCGC 8 cut(s) 99, 289, 291, 4239, 4360, 4673, 6309, 6581
HinP1I GCGC 8 cut(s) 99, 289, 291, 4239, 4360, 4673, 6309, 6581
HincII GTYRAC 3 cut(s) 2836, 3355, 6082
HindII GTYRAC 3 cut(s) 2836, 3355, 6082
HindIII AAGCTT 5 cut(s) 4134, 4292, 4335, 5381, 6617
HpaII CCGG 5 cut(s) 197, 527, 3148, 3358, 4279
Hpy99I CGWCG 3 cut(s) 227, 245, 248
HpyCH4IV ACGT 9 cut(s) 1111, 2314, 2350, 2585, 2755, 3567, 4062, 5339, 5530
HpySE526I ACGT 9 cut(s) 1111, 2314, 2350, 2585, 2755, 3567, 4062, 5339, 5530
Hsp92I GRCGYC 1 cut(s) 246
HspAI GCGC 8 cut(s) 99, 289, 291, 4239, 4360, 4673, 6309, 6581
KroI GCCGGC 1 cut(s) 4278
KroNI GCCGGC 1 cut(s) 4280
Ksp22I TGATCA 4 cut(s) 553, 1996, 2179, 3382
LguI GCTCTTC 2 cut(s) 399, 5775
MabI ACCWGGT 1 cut(s) 6993
MaeII ACGT 9 cut(s) 1111, 2314, 2350, 2585, 2755, 3567, 4062, 5339, 5530
MbiI CCGCTC 3 cut(s) 155, 409, 1676
MfeI CAATTG 7 cut(s) 1758, 2241, 2922, 3045, 3800, 5282, 5823
MflI RGATCY 8 cut(s) 898, 1073, 3121, 4167, 4306, 5975, 6247, 6722
MlsI TGGCCA 2 cut(s) 695, 6138
MluI ACGCGT 1 cut(s) 5151
MluNI TGGCCA 2 cut(s) 695, 6138
MlyI GAGTC 5 cut(s) 2723, 3560, 4358, 5609, 6776
MmeI TCCRAC 2 cut(s) 620, 6529
Mox20I TGGCCA 2 cut(s) 695, 6138
Mph1103I ATGCAT 5 cut(s) 4035, 5299, 5443, 5707, 6318
MroNI GCCGGC 1 cut(s) 4278
MroXI GAANNNNTTC 4 cut(s) 1113, 4912, 5010, 5073
MscI TGGCCA 2 cut(s) 695, 6138
MslI CAYNNNNRTG 7 cut(s) 701, 783, 1961, 1992, 4543, 6144, 6573
Msp20I TGGCCA 2 cut(s) 695, 6138
MspI CCGG 5 cut(s) 197, 527, 3148, 3358, 4279
MunI CAATTG 7 cut(s) 1758, 2241, 2922, 3045, 3800, 5282, 5823
Mva1269I GAATGC 4 cut(s) 1610, 5299, 5707, 6848
MvnI CGCG 7 cut(s) 99, 291, 835, 4241, 4362, 5153, 5751
NaeI GCCGGC 1 cut(s) 4280
NciI CCSGG 2 cut(s) 197, 3149
NcoI CCATGG 4 cut(s) 1350, 4389, 4544, 4953
NdeI CATATG 1 cut(s) 1989
NgoMIV GCCGGC 1 cut(s) 4278
NheI GCTAGC 1 cut(s) 1301
NmeAIII GCCGAG 4 cut(s) 36, 319, 4471, 5342
NmuCI GTSAC 3 cut(s) 3050, 4097, 6622
NsiI ATGCAT 5 cut(s) 4035, 5299, 5443, 5707, 6318
NspV TTCGAA 1 cut(s) 402
PaeI GCATGC 2 cut(s) 1753, 5129
PagI TCATGA 2 cut(s) 1956, 3079
PaqCI CACCTGC 2 cut(s) 1248, 4095
PauI GCGCGC 1 cut(s) 289
PciI ACATGT 4 cut(s) 1206, 2057, 2087, 5663
PciSI GCTCTTC 2 cut(s) 399, 5775
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 4 cut(s) 1610, 5299, 5707, 6848
PdiI GCCGGC 1 cut(s) 4280
PdmI GAANNNNTTC 4 cut(s) 1113, 4912, 5010, 5073
PflFI GACNNNGTC 3 cut(s) 1588, 6479, 6995
PflMI CCANNNNNTGG 4 cut(s) 1356, 2589, 2740, 7000
PfoI TCCNGGA 2 cut(s) 2557, 4965
PinAI ACCGGT 1 cut(s) 3357
Ple19I CGATCG 1 cut(s) 3847
PleI GAGTC 5 cut(s) 2723, 3559, 4358, 5608, 6776
PpsI GAGTC 5 cut(s) 2723, 3559, 4358, 5608, 6776
Ppu21I YACGTR 2 cut(s) 2351, 2586
PpuMI RGGWCCY 1 cut(s) 2516
PscI ACATGT 4 cut(s) 1206, 2057, 2087, 5663
PshAI GACNNNNGTC 1 cut(s) 140
PshBI ATTAAT 2 cut(s) 1397, 7014
Psp124BI GAGCTC 3 cut(s) 231, 2805, 3320
Psp5II RGGWCCY 1 cut(s) 2516
PspEI GGTNACC 1 cut(s) 4895
PspPPI RGGWCCY 1 cut(s) 2516
PstI CTGCAG 7 cut(s) 975, 1384, 1917, 2530, 4275, 4306, 6397
PstNI CAGNNNCTG 3 cut(s) 1408, 3923, 5191
PsuI RGATCY 8 cut(s) 898, 1073, 3121, 4167, 4306, 5975, 6247, 6722
PsyI GACNNNGTC 3 cut(s) 1588, 6479, 6995
PteI GCGCGC 1 cut(s) 289
PvuI CGATCG 1 cut(s) 3847
PvuII CAGCTG 9 cut(s) 1265, 1405, 3167, 4080, 5776, 5855, 6392, 6881, 6940
RseI CAYNNNNRTG 7 cut(s) 701, 783, 1961, 1992, 4543, 6144, 6573
Rsr2I CGGWCCG 1 cut(s) 2173
RsrII CGGWCCG 1 cut(s) 2173
SacI GAGCTC 3 cut(s) 231, 2805, 3320
SapI GCTCTTC 2 cut(s) 399, 5775
SchI GAGTC 5 cut(s) 2723, 3560, 4358, 5609, 6776
SexAI ACCWGGT 1 cut(s) 6993
SfcI CTRYAG 9 cut(s) 971, 1380, 1913, 2472, 2526, 3338, 4271, 4302, 6393
SfuI TTCGAA 1 cut(s) 402
SmiMI CAYNNNNRTG 7 cut(s) 701, 783, 1961, 1992, 4543, 6144, 6573
SmlI CTYRAG 8 cut(s) 592, 905, 2135, 2532, 2639, 4022, 4592, 5807
SmoI CTYRAG 8 cut(s) 592, 905, 2135, 2532, 2639, 4022, 4592, 5807
SpeI ACTAGT 3 cut(s) 538, 2933, 3965
SphI GCATGC 2 cut(s) 1753, 5129
SspI AATATT 2 cut(s) 966, 1309
SstI GAGCTC 3 cut(s) 231, 2805, 3320
StyI CCWWGG 8 cut(s) 1350, 3117, 3321, 3613, 4389, 4509, 4544, 4953
TaiI ACGT 9 cut(s) 1114, 2317, 2353, 2588, 2758, 3570, 4065, 5342, 5533
TatI WGTACW 8 cut(s) 646, 766, 2476, 3288, 3730, 5266, 5344, 5778
TauI GCSGC 2 cut(s) 41, 286
TseFI GTSAC 3 cut(s) 3050, 4097, 6622
Tsp45I GTSAC 3 cut(s) 3050, 4097, 6622
Tth111I GACNNNGTC 3 cut(s) 1588, 6479, 6995
Van91I CCANNNNNTGG 4 cut(s) 1356, 2589, 2740, 7000
VneI GTGCAC 2 cut(s) 4782, 5889
VspI ATTAAT 2 cut(s) 1397, 7014
XagI CCTNNNNNAGG 2 cut(s) 478, 1783
XapI RAATTY 7 cut(s) 1092, 1567, 1721, 4646, 6556, 6866, 6947
XbaI TCTAGA 2 cut(s) 1825, 5578
XcmI CCANNNNNNNNNTGG 3 cut(s) 3920, 5233, 6485
XmiI GTMKAC 1 cut(s) 6417
XmnI GAANNNNTTC 4 cut(s) 1113, 4912, 5010, 5073
Zsp2I ATGCAT 5 cut(s) 4035, 5299, 5443, 5707, 6318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.