AT2G27450

N-carbamoylputrescine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006306
Physical Location & Seq
Reverse (-)
11737374 .. 11739690
2317 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27450.2

Sequence Viewer

Length: 900 bp
ATGGAAACGGAAGGAAGAAGACGAGAAGTGGTTGTTTCATCTCTCCAATTCGCTTGCTCCGACGATATCTCCACCAATGTCGCCGCCGCTGAGCGATTAGTCAGAGAAGCTCACGCTAAAGGAGCTAATATCATTCTTATTCAGGAACTTTTTGAAGGATATTACTTCTGTCAAGCACAAAGAGAAGACTTCTTTAAGCGAGCCAAGCCTTACAAGAACCATCCTACCATTGCAAGGATGCAGAAGCTGGCAAAGGAATTGGGTGTAGTGATACCTGTTAGTTTCTTTGAGGAGGCAAATACTGCACATTACAATTCGATTGCTATAATTGATGCTGATGGAACTGACTTGGGAATTTATAGGAAGTCTCATATTCCTGATGGACCTGGTTATCAAGAGAAGTTTTATTTCAATCCTGGAGATACTGGCTTTAAGGTTTTCCAGACGAAGTTTGCTAAAATTGGAGTGGCTATATGTTGGGATCAGTGGTTTCCTGAGGCAGCTCGAGCTATGGTTCTACAGGGTGCTGAAATACTGTTTTATCCCACTGCTATTGGTTCTGAACCTCAAGACCAGGGATTGGATTCGCGTGATCATTGGAGGAGAGTTATGCAAGGGCATGCTGGAGCTAACGTGGTACCTCTAGTAGCCTCAAATCGCATAGGGAAGGAAATAATTGAAACTGAGCATGGACCTAGTCAGATCACTTTCTACGGAACTTCCTTCATTGCCGGACCAACAGGTGAAATTGTGGCCGAGGCAGATGATAAATCGGAAGCTGTTCTTGTAGCACAGTTTGATCTTGATATGATCAAGTCGAAAAGGCAAAGCTGGGGAGTGTTCCGTGACCGTCGTCCAGATTTGTACAAGGTGCTTCTTACTATGGATGGTAATCTCTAA

Protein Analysis

299

Amino Acids

33.53

Weight (kDa)

5.71

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 60 - 302 1.4e-54 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 637
AccB1I GGYRCC 1 cut(s) 637
AccB7I CCANNNNNTGG 1 cut(s) 580
AccII CGCG 1 cut(s) 589
AciI CCGC 2 cut(s) 84, 87
AclWI GGATC 1 cut(s) 489
AcoI YGGCCR 1 cut(s) 753
AcsI RAATTY 1 cut(s) 354
AfaI GTAC 2 cut(s) 639, 866
AfiI CCNNNNNNNGG 2 cut(s) 234, 580
AgsI TTSAA 3 cut(s) 155, 412, 680
AjnI CCWGG 3 cut(s) 385, 415, 573
AluBI AGCT 8 cut(s) 110, 125, 247, 503, 509, 629, 779, 831
AluI AGCT 8 cut(s) 110, 125, 247, 503, 509, 629, 779, 831
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 1 cut(s) 489
AlwNI CAGNNNCTG 1 cut(s) 247
Ama87I CYCGRG 1 cut(s) 504
AoxI GGCC 1 cut(s) 753
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 780
Asp718I GGTACC 1 cut(s) 637
AspS9I GGNCC 3 cut(s) 383, 692, 734
AsuHPI GGTGA 1 cut(s) 755
AvaI CYCGRG 1 cut(s) 504
AvaII GGWCC 3 cut(s) 383, 692, 734
AxyI CCTNAGG 1 cut(s) 495
BanI GGYRCC 1 cut(s) 637
BbsI GAAGAC 2 cut(s) 25, 192
BbvI GCAGC 1 cut(s) 512
BccI CCATC 4 cut(s) 228, 332, 374, 881
BciT130I CCWGG 3 cut(s) 387, 417, 575
BclI TGATCA 2 cut(s) 592, 810
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 2 cut(s) 644, 696
BfmI CTRYAG 1 cut(s) 518
BisI GCNGC 3 cut(s) 84, 87, 501
BlpI GCTNAGC 1 cut(s) 90
BlsI GCNGC 3 cut(s) 85, 88, 502
Bme1390I CCNGG 3 cut(s) 387, 417, 575
Bme18I GGWCC 3 cut(s) 383, 692, 734
BmeT110I CYCGRG 1 cut(s) 504
BmgT120I GGNCC 3 cut(s) 383, 692, 734
BmiI GGNNCC 1 cut(s) 639
BmrFI CCNGG 3 cut(s) 387, 417, 575
BmsI GCATC 2 cut(s) 228, 322
BoxI GACNNNNGTC 1 cut(s) 852
BpiI GAAGAC 2 cut(s) 25, 192
BpmI CTGGAG 2 cut(s) 438, 645
Bpu1102I GCTNAGC 1 cut(s) 90
BpuEI CTTGAG 1 cut(s) 552
BsaBI GATNNNNATC 1 cut(s) 891
BsaJI CCNNGG 2 cut(s) 574, 756
BsaXI ACNNNNNCTCC 4 cut(s) 53, 83, 618, 648
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 580
Bse1I ACTGG 1 cut(s) 430
Bse21I CCTNAGG 1 cut(s) 495
Bse3DI GCAATG 2 cut(s) 228, 726
Bse8I GATNNNNATC 1 cut(s) 891
BseBI CCWGG 3 cut(s) 387, 417, 575
BseDI CCNNGG 2 cut(s) 574, 756
BseGI GGATG 3 cut(s) 220, 243, 892
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 2 cut(s) 234, 580
BseMI GCAATG 2 cut(s) 228, 726
BseMII CTCAG 3 cut(s) 81, 486, 675
BseNI ACTGG 1 cut(s) 430
BseRI GAGGAG 2 cut(s) 305, 616
BseXI GCAGC 1 cut(s) 512
BseYI CCCAGC 1 cut(s) 831
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 1 cut(s) 589
BshFI GGCC 1 cut(s) 755
BshNI GGYRCC 1 cut(s) 637
BsiHKCI CYCGRG 1 cut(s) 504
BsiSI CCGG 1 cut(s) 732
BslI CCNNNNNNNGG 2 cut(s) 234, 580
BsmAI GTCTC 1 cut(s) 372
BsnI GGCC 1 cut(s) 755
BsoBI CYCGRG 1 cut(s) 504
Bsp1407I TGTACA 1 cut(s) 864
Bsp143I GATC 5 cut(s) 481, 592, 702, 799, 810
Bsp1720I GCTNAGC 1 cut(s) 90
BspACI CCGC 2 cut(s) 84, 87
BspANI GGCC 1 cut(s) 755
BspCNI CTCAG 3 cut(s) 82, 487, 676
BspFNI CGCG 1 cut(s) 589
BspLI GGNNCC 1 cut(s) 639
BspPI GGATC 1 cut(s) 489
BspT107I GGYRCC 1 cut(s) 637
BsrDI GCAATG 2 cut(s) 228, 726
BsrGI TGTACA 1 cut(s) 864
BsrI ACTGG 1 cut(s) 430
BssECI CCNNGG 2 cut(s) 574, 756
BssMI GATC 5 cut(s) 481, 592, 702, 799, 810
Bst2UI CCWGG 3 cut(s) 387, 417, 575
Bst4CI ACNGT 3 cut(s) 537, 795, 851
BstAPI GCANNNNNTGC 1 cut(s) 302
BstAUI TGTACA 1 cut(s) 864
BstC8I GCNNGC 4 cut(s) 55, 201, 249, 621
BstDEI CTNAG 3 cut(s) 90, 495, 684
BstF5I GGATG 3 cut(s) 220, 243, 892
BstFNI CGCG 1 cut(s) 589
BstKTI GATC 5 cut(s) 484, 595, 705, 802, 813
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 5 cut(s) 481, 592, 702, 799, 810
BstMWI GCNNNNNNNGC 4 cut(s) 122, 205, 302, 506
BstNI CCWGG 3 cut(s) 387, 417, 575
BstNSI RCATGY 1 cut(s) 623
BstPAI GACNNNNGTC 1 cut(s) 852
BstSCI CCNGG 3 cut(s) 385, 415, 573
BstSFI CTRYAG 1 cut(s) 518
BstUI CGCG 1 cut(s) 589
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 2 cut(s) 25, 192
Bsu36I CCTNAGG 1 cut(s) 495
BsuRI GGCC 1 cut(s) 755
BtsCI GGATG 3 cut(s) 220, 243, 892
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 2 cut(s) 491, 546
Cac8I GCNNGC 4 cut(s) 55, 201, 249, 621
CaiI CAGNNNCTG 1 cut(s) 247
Cfr13I GGNCC 3 cut(s) 383, 692, 734
CsiI ACCWGGT 1 cut(s) 385
Csp6I GTAC 2 cut(s) 638, 865
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviAII CATG 2 cut(s) 620, 689
CviQI GTAC 2 cut(s) 638, 865
DdeI CTNAG 3 cut(s) 90, 495, 684
DpnI GATC 5 cut(s) 483, 594, 704, 801, 812
DpnII GATC 5 cut(s) 481, 592, 702, 799, 810
EaeI YGGCCR 1 cut(s) 753
Eco32I GATATC 1 cut(s) 67
Eco47I GGWCC 3 cut(s) 383, 692, 734
Eco81I CCTNAGG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 504
EcoRII CCWGG 3 cut(s) 385, 415, 573
EcoRV GATATC 1 cut(s) 67
FaeI CATG 2 cut(s) 623, 692
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FatI CATG 2 cut(s) 619, 688
FbaI TGATCA 2 cut(s) 592, 810
Fnu4HI GCNGC 3 cut(s) 84, 87, 501
FokI GGATG 2 cut(s) 207, 250
Fsp4HI GCNGC 3 cut(s) 84, 87, 501
FspBI CTAG 2 cut(s) 644, 696
GluI GCNGC 3 cut(s) 84, 87, 501
GsaI CCCAGC 1 cut(s) 835
GsuI CTGGAG 2 cut(s) 438, 645
HaeIII GGCC 1 cut(s) 755
HapII CCGG 1 cut(s) 732
Hin1II CATG 2 cut(s) 623, 692
HinfI GANTC 1 cut(s) 584
HpaII CCGG 1 cut(s) 732
HphI GGTGA 1 cut(s) 755
Hpy188I TCNGA 5 cut(s) 61, 104, 562, 702, 775
Hpy188III TCNNGA 8 cut(s) 143, 377, 395, 442, 494, 569, 803, 857
Hpy99I CGWCG 2 cut(s) 65, 855
HpyAV CCTTC 4 cut(s) 5, 149, 661, 733
HpyCH4III ACNGT 3 cut(s) 537, 795, 851
HpyCH4IV ACGT 1 cut(s) 633
HpyCH4V TGCA 4 cut(s) 233, 241, 305, 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 205, 302, 506
HpyF3I CTNAG 3 cut(s) 90, 495, 684
HpySE526I ACGT 1 cut(s) 633
Hsp92II CATG 2 cut(s) 623, 692
KpnI GGTACC 1 cut(s) 641
Ksp22I TGATCA 2 cut(s) 592, 810
Kzo9I GATC 5 cut(s) 481, 592, 702, 799, 810
LmnI GCTCC 3 cut(s) 62, 122, 626
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 2 cut(s) 228, 322
MabI ACCWGGT 1 cut(s) 385
MaeI CTAG 2 cut(s) 644, 696
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 1 cut(s) 845
MalI GATC 5 cut(s) 483, 594, 704, 801, 812
MboI GATC 5 cut(s) 481, 592, 702, 799, 810
MboII GAAGA 3 cut(s) 27, 30, 197
MluCI AATT 8 cut(s) 47, 257, 313, 327, 354, 459, 675, 747
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 8 cut(s) 283, 286, 490, 576, 594, 651, 661, 751
MroXI GAANNNNTTC 1 cut(s) 780
MseI TTAA 2 cut(s) 195, 432
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 1 cut(s) 732
MspR9I CCNGG 3 cut(s) 387, 417, 575
MvaI CCWGG 3 cut(s) 387, 417, 575
MvnI CGCG 1 cut(s) 589
MwoI GCNNNNNNNGC 4 cut(s) 122, 205, 302, 506
NdeII GATC 5 cut(s) 481, 592, 702, 799, 810
NlaIII CATG 2 cut(s) 623, 692
NlaIV GGNNCC 1 cut(s) 639
NmeAIII GCCGAG 1 cut(s) 781
NmuCI GTSAC 1 cut(s) 845
NspI RCATGY 1 cut(s) 623
PaeI GCATGC 1 cut(s) 623
PaeR7I CTCGAG 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 780
PfeI GAWTC 1 cut(s) 584
PflFI GACNNNGTC 1 cut(s) 696
PflMI CCANNNNNTGG 1 cut(s) 580
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 3 cut(s) 85, 88, 502
PshAI GACNNNNGTC 1 cut(s) 852
Psp6I CCWGG 3 cut(s) 385, 415, 573
PspFI CCCAGC 1 cut(s) 831
PspGI CCWGG 3 cut(s) 385, 415, 573
PspN4I GGNNCC 1 cut(s) 639
PspPI GGNCC 3 cut(s) 383, 692, 734
PspXI VCTCGAGB 1 cut(s) 504
PstNI CAGNNNCTG 1 cut(s) 247
PsyI GACNNNGTC 1 cut(s) 696
RsaI GTAC 2 cut(s) 639, 866
RsaNI GTAC 2 cut(s) 638, 865
SaqAI TTAA 2 cut(s) 195, 432
SatI GCNGC 3 cut(s) 84, 87, 501
Sau3AI GATC 5 cut(s) 481, 592, 702, 799, 810
Sau96I GGNCC 3 cut(s) 383, 692, 734
ScrFI CCNGG 3 cut(s) 387, 417, 575
SexAI ACCWGGT 1 cut(s) 385
SfaNI GCATC 2 cut(s) 228, 322
SfcI CTRYAG 1 cut(s) 518
Sfr274I CTCGAG 1 cut(s) 504
SinI GGWCC 3 cut(s) 383, 692, 734
SlaI CTCGAG 1 cut(s) 504
SmlI CTYRAG 2 cut(s) 504, 567
SmoI CTYRAG 2 cut(s) 504, 567
SphI GCATGC 1 cut(s) 623
Sse9I AATT 8 cut(s) 47, 257, 313, 327, 354, 459, 675, 747
SsiI CCGC 2 cut(s) 84, 87
SspMI CTAG 2 cut(s) 644, 696
StyD4I CCNGG 3 cut(s) 385, 415, 573
TaaI ACNGT 3 cut(s) 537, 795, 851
TaiI ACGT 1 cut(s) 636
TaqI TCGA 3 cut(s) 317, 505, 818
TasI AATT 8 cut(s) 47, 257, 313, 327, 354, 459, 675, 747
TatI WGTACW 1 cut(s) 864
TauI GCSGC 2 cut(s) 86, 89
TfiI GAWTC 1 cut(s) 584
Tru1I TTAA 2 cut(s) 195, 432
Tru9I TTAA 2 cut(s) 195, 432
TscAI CASTG 2 cut(s) 491, 553
TseFI GTSAC 1 cut(s) 845
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 1 cut(s) 845
TspDTI ATGAA 2 cut(s) 27, 715
TspGWI ACGGA 3 cut(s) 23, 729, 833
TspRI CASTG 2 cut(s) 491, 553
Tth111I GACNNNGTC 1 cut(s) 696
Van91I CCANNNNNTGG 1 cut(s) 580
VpaK11BI GGWCC 3 cut(s) 383, 692, 734
XapI RAATTY 1 cut(s) 354
XceI RCATGY 1 cut(s) 623
XhoI CTCGAG 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 780
XspI CTAG 2 cut(s) 644, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.