Rh5DG324300

N-carbamoylputrescine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006306
Physical Location & Seq
Forward (+)
42317902 .. 42326358
8457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG324300.1

Sequence Viewer

Length: 693 bp
ATGGAAAACAAGGGTAAGAGGGAGGTGGTGGTCTCCGCTTTGCAGTTCGCTTGCACCGACGACGTCGCCACCAACGTCGCCACCGCCGAAAGGTTGGTTAGAGCTGCTCATTCAAAGGGTGCAAACATCATTCTTATACAGGAGCTTTTTGAAGGATATTACTTCTGTCAAGCACAAAGGGAGGACTTCATTCAGCGAGCAAAGCCTTACAGCAACCATCCAACTATTCTTAGGATGCAGAAACTTGCAAAAGAGTTGGGTGTGGTAATACCAGTTAGCTTTTTTGAAGAGGCGAACAATGCCCACTACAATTCAATTGCCATTATTGATGCAGACGGTGCCGATCTTGGGCTTTATAGAAAGTCCCACATCCCAGATGGACCAGGCTACCAGGAAAAGTTCTACTTCAATCCAGGTGACACTGGATTCAAGGTTTTCCAGACCAAGTTCGCAAAAATAGGAGTTGCAATTTGCTGGGATCAATGGTTTCCTGAGGCAGCCCGAGCTTTGGTTCTTCAAGGTGCAGAGATTTTGTTTTATCCTACTGCTATTGGTTCTGAACCTCAAGATGGAGGACTTGATTCTAGGGATCACTGGAGGCGAGTAATGCAAGGGCATGCTGGGGCGAATTTGGTAATTTTCTTAGATTTTAATGCCATCAACTACAGCTGGGATGGATATTGGGGAGCATGA

Protein Analysis

230

Amino Acids

25.79

Weight (kDa)

5.68

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 13 - 209 2.4e-41 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
AccB1I GGYRCC 1 cut(s) 338
AciI CCGC 2 cut(s) 36, 84
AclWI GGATC 2 cut(s) 486, 597
AcsI RAATTY 1 cut(s) 628
AcyI GRCGYC 1 cut(s) 63
AfiI CCNNNNNNNGG 4 cut(s) 90, 348, 508, 569
AgsI TTSAA 7 cut(s) 114, 152, 287, 315, 409, 430, 518
AjnI CCWGG 3 cut(s) 382, 390, 412
AluBI AGCT 5 cut(s) 104, 145, 279, 506, 669
AluI AGCT 5 cut(s) 104, 145, 279, 506, 669
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 2 cut(s) 486, 597
Ama87I CYCGRG 1 cut(s) 501
ApeKI GCWGC 2 cut(s) 104, 497
ApoI RAATTY 1 cut(s) 628
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 501
AvaII GGWCC 1 cut(s) 380
AxyI CCTNAGG 1 cut(s) 492
BanI GGYRCC 1 cut(s) 338
BbvI GCAGC 2 cut(s) 91, 509
BccI CCATC 5 cut(s) 225, 371, 563, 665, 668
BciT130I CCWGG 3 cut(s) 384, 392, 414
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 1 cut(s) 585
BfmI CTRYAG 1 cut(s) 664
BisI GCNGC 2 cut(s) 105, 498
BlsI GCNGC 2 cut(s) 106, 499
Bme1390I CCNGG 3 cut(s) 384, 392, 414
Bme18I GGWCC 1 cut(s) 380
BmeT110I CYCGRG 1 cut(s) 501
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 3 cut(s) 384, 392, 414
BmsI GCATC 2 cut(s) 225, 319
BpmI CTGGAG 1 cut(s) 616
BpuEI CTTGAG 1 cut(s) 549
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 37
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 348, 508, 569
Bse1I ACTGG 3 cut(s) 272, 427, 599
Bse21I CCTNAGG 1 cut(s) 492
BseBI CCWGG 3 cut(s) 384, 392, 414
BseGI GGATG 4 cut(s) 217, 240, 369, 679
BseLI CCNNNNNNNGG 4 cut(s) 90, 348, 508, 569
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 3 cut(s) 272, 427, 599
BseXI GCAGC 2 cut(s) 91, 509
BseYI CCCAGC 3 cut(s) 474, 620, 669
BsgI GTGCAG 1 cut(s) 543
BshNI GGYRCC 1 cut(s) 338
BsiHKCI CYCGRG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 4 cut(s) 90, 348, 508, 569
BsmAI GTCTC 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 349
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 1 cut(s) 501
Bsp143I GATC 3 cut(s) 343, 478, 589
BspACI CCGC 2 cut(s) 36, 84
BspCNI CTCAG 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 340
BspPI GGATC 2 cut(s) 486, 597
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 37
BsrI ACTGG 3 cut(s) 272, 427, 599
BssMI GATC 3 cut(s) 343, 478, 589
BssNI GRCGYC 1 cut(s) 63
Bst2UI CCWGG 3 cut(s) 384, 392, 414
Bst4CI ACNGT 1 cut(s) 338
Bst6I CTCTTC 1 cut(s) 282
BstACI GRCGYC 1 cut(s) 63
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 52, 198, 618
BstDEI CTNAG 3 cut(s) 230, 492, 643
BstF5I GGATG 4 cut(s) 217, 240, 369, 679
BstKTI GATC 3 cut(s) 346, 481, 592
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 3 cut(s) 343, 478, 589
BstMWI GCNNNNNNNGC 5 cut(s) 202, 299, 338, 503, 607
BstNI CCWGG 3 cut(s) 384, 392, 414
BstNSI RCATGY 1 cut(s) 620
BstSCI CCNGG 3 cut(s) 382, 390, 412
BstSFI CTRYAG 1 cut(s) 664
BstV1I GCAGC 2 cut(s) 91, 509
Bsu36I CCTNAGG 1 cut(s) 492
BtsCI GGATG 4 cut(s) 217, 240, 369, 679
BtsIMutI CAGTG 2 cut(s) 420, 592
Cac8I GCNNGC 3 cut(s) 52, 198, 618
Cfr13I GGNCC 1 cut(s) 380
CviAII CATG 2 cut(s) 617, 690
CviJI RGCY 9 cut(s) 104, 145, 205, 279, 352, 387, 500, 506, 669
CviKI_1 RGCY 9 cut(s) 104, 145, 205, 279, 352, 387, 500, 506, 669
DdeI CTNAG 3 cut(s) 230, 492, 643
DpnI GATC 3 cut(s) 345, 480, 591
DpnII GATC 3 cut(s) 343, 478, 589
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 380
Eco81I CCTNAGG 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 501
EcoRII CCWGG 3 cut(s) 382, 390, 412
FaeI CATG 2 cut(s) 620, 693
FaiI YATR 4 cut(s) 137, 357, 618, 691
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 1 cut(s) 349
FatI CATG 2 cut(s) 616, 689
Fnu4HI GCNGC 2 cut(s) 105, 498
FokI GGATG 4 cut(s) 204, 247, 356, 686
Fsp4HI GCNGC 2 cut(s) 105, 498
FspBI CTAG 1 cut(s) 585
GluI GCNGC 2 cut(s) 105, 498
GsaI CCCAGC 3 cut(s) 478, 624, 673
GsuI CTGGAG 1 cut(s) 616
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 2 cut(s) 620, 693
HinfI GANTC 2 cut(s) 426, 581
HphI GGTGA 1 cut(s) 428
Hpy188I TCNGA 1 cut(s) 559
Hpy188III TCNNGA 3 cut(s) 439, 491, 566
Hpy99I CGWCG 4 cut(s) 62, 65, 68, 80
HpyAV CCTTC 1 cut(s) 146
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4IV ACGT 2 cut(s) 63, 75
HpyCH4V TGCA 9 cut(s) 43, 54, 122, 238, 248, 332, 467, 524, 610
HpyF10VI GCNNNNNNNGC 5 cut(s) 202, 299, 338, 503, 607
HpyF3I CTNAG 3 cut(s) 230, 492, 643
HpySE526I ACGT 2 cut(s) 63, 75
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 2 cut(s) 620, 693
Kzo9I GATC 3 cut(s) 343, 478, 589
LmnI GCTCC 2 cut(s) 142, 686
Lsp1109I GCAGC 2 cut(s) 91, 509
LweI GCATC 2 cut(s) 225, 319
MaeI CTAG 1 cut(s) 585
MaeII ACGT 2 cut(s) 63, 75
MaeIII GTNAC 1 cut(s) 416
MalI GATC 3 cut(s) 345, 480, 591
MboI GATC 3 cut(s) 343, 478, 589
MboII GAAGA 2 cut(s) 299, 506
MfeI CAATTG 1 cut(s) 315
MluCI AATT 5 cut(s) 310, 315, 468, 628, 636
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 8 cut(s) 12, 16, 175, 283, 487, 566, 573, 591
MseI TTAA 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 669
MspR9I CCNGG 3 cut(s) 384, 392, 414
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 3 cut(s) 384, 392, 414
MwoI GCNNNNNNNGC 5 cut(s) 202, 299, 338, 503, 607
NdeII GATC 3 cut(s) 343, 478, 589
NlaIII CATG 2 cut(s) 620, 693
NlaIV GGNNCC 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 1 cut(s) 620
PaeI GCATGC 1 cut(s) 620
PcsI WCGNNNNNNNCGW 3 cut(s) 54, 72, 84
PfeI GAWTC 2 cut(s) 426, 581
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 106, 499
Psp6I CCWGG 3 cut(s) 382, 390, 412
PspFI CCCAGC 3 cut(s) 474, 620, 669
PspGI CCWGG 3 cut(s) 382, 390, 412
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 380
PsyI GACNNNGTC 1 cut(s) 62
PvuII CAGCTG 1 cut(s) 669
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 2 cut(s) 105, 498
Sau3AI GATC 3 cut(s) 343, 478, 589
Sau96I GGNCC 1 cut(s) 380
ScrFI CCNGG 3 cut(s) 384, 392, 414
SfaNI GCATC 2 cut(s) 225, 319
SfcI CTRYAG 1 cut(s) 664
SinI GGWCC 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 564
SmoI CTYRAG 1 cut(s) 564
SphI GCATGC 1 cut(s) 620
Sse9I AATT 5 cut(s) 310, 315, 468, 628, 636
SsiI CCGC 2 cut(s) 36, 84
SspMI CTAG 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 382, 390, 412
TaaI ACNGT 1 cut(s) 338
TaiI ACGT 2 cut(s) 66, 78
TasI AATT 5 cut(s) 310, 315, 468, 628, 636
TfiI GAWTC 2 cut(s) 426, 581
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 2 cut(s) 427, 599
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 2 cut(s) 104, 497
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 1 cut(s) 178
TspRI CASTG 2 cut(s) 427, 599
Tth111I GACNNNGTC 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 1 cut(s) 628
XceI RCATGY 1 cut(s) 620
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XspI CTAG 1 cut(s) 585
ZraI GACGTC 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.