Rroxscaffold_1G00035570

N-carbamoylputrescine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006306
Physical Location & Seq
Reverse (-)
51676088 .. 51681790
5703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035570.1

Sequence Viewer

Length: 1122 bp
ATGGAAAACAAGGGTAAGAGGGAGGTGGTGGTCTCAGCTTTGCAGTTCGCTTGCACCGACGACGTCGCCACCAACGTCGCCACCGCCGAAAGGTTGGTTAGAGCTGCTCATTCAAAGGGTGCAAACATCATTCTTATACAGGAGCTTTTTGAAGGATATTACTTCTGTCAAGCACAAAGGGAGGACTTCATTCAGCGAGCAAAGCCTTACAGCAACCATCCAACTATTCTTAGGATGCAGAAACTTGCAAAAGAGTTGGGTGTGGTAATACCAGTTAGCTTTTTTGAAGAGGCGAACAATGCCCACTACAATTCAATTGCCATTATTGATGCAGATGGTGCCGATCTTGGACTTTATAGAAAGTCCCACATCCCAGATGGACCAGGAAAGTTCTACTTCAATCCAGGAGACACTGGATTCAAGGTTTTCCAGACCAAGTTCGCAAAAATAGGAGTTGGTGAGAAAACTAAGCAAGGCTGGCAATTTATTTGGCTAATACATCATTTAATTTCAGCAATTTGCTGGGATCAATGGTTTCCTGAGGCAGCCCGAGCTTTGGTTCTTCAAGGTGCAGAGATTTTGTTTTATCCTACTGCTATTGGTTCTGAACCTCAAGATGGAGGACTTGATTCTAGGGATCACTGGAGGCGAGTAATGCAAGGGCATGCTGGGGCGAATTTGGTGCCTGTAGTAACATCAAACCGCATAGGAAAGGAGATAATTGTGACTGAGCATGGAAAGAGTGAGATCACATTTTATGGTAACTCTTTCATAGCAGGACCTACTGGAGAAATTGTTACAGCTGCTAATGATAAAGATGAAGCGGTACTAGTGGCAGAATTTGATTTGGATAAGATCAAATGCAAGAGGCATAGCTGGGGAGTATTCCGTGATCGACGTCCAGAGTTATACAAGTGTATGGAATATTTTATGTCTTTCGCTGCCGGTTCAGTTGATAAAACAGACCTAATGGTGAAGATACTTGGAATATTCCAACTGAGTAACTGTGATGATACTCAGTATGGATGGATGAGGTCTTCTGAAGCACTAGAGCCCCAGATGTGGACTCATTTCGTCCAGATTAATGCATTGCCCAAAGTCAAGTTTTTTCGAAGGCGATAA

Protein Analysis

373

Amino Acids

42.18

Weight (kDa)

7.17

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 13 - 286 9e-45 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 66, 901
AccB1I GGYRCC 2 cut(s) 338, 682
AciI CCGC 3 cut(s) 84, 703, 824
AclWI GGATC 2 cut(s) 534, 645
AcsI RAATTY 2 cut(s) 676, 839
AcuI CTGAAG 1 cut(s) 1062
AcyI GRCGYC 2 cut(s) 63, 898
AfaI GTAC 1 cut(s) 828
AfiI CCNNNNNNNGG 4 cut(s) 90, 556, 617, 1062
AgsI TTSAA 7 cut(s) 114, 152, 287, 315, 400, 421, 566
AhlI ACTAGT 1 cut(s) 829
AjnI CCWGG 2 cut(s) 382, 403
AluBI AGCT 7 cut(s) 38, 104, 145, 279, 554, 803, 876
AluI AGCT 7 cut(s) 38, 104, 145, 279, 554, 803, 876
Alw26I GTCTC 2 cut(s) 37, 402
AlwI GGATC 2 cut(s) 534, 645
Ama87I CYCGRG 1 cut(s) 549
ApeKI GCWGC 4 cut(s) 104, 545, 803, 941
ApoI RAATTY 2 cut(s) 676, 839
AseI ATTAAT 1 cut(s) 1083
AspS9I GGNCC 2 cut(s) 380, 779
AsuHPI GGTGA 2 cut(s) 470, 985
AsuII TTCGAA 1 cut(s) 1111
AvaI CYCGRG 1 cut(s) 549
AvaII GGWCC 2 cut(s) 380, 779
AxyI CCTNAGG 1 cut(s) 540
BanI GGYRCC 2 cut(s) 338, 682
BanII GRGCYC 1 cut(s) 1056
BbsI GAAGAC 1 cut(s) 1029
BbvI GCAGC 4 cut(s) 91, 557, 790, 928
BccI CCATC 5 cut(s) 225, 329, 371, 611, 1020
BcgI CGANNNNNNTGC 2 cut(s) 664, 698
BciT130I CCWGG 2 cut(s) 384, 405
BcoDI GTCTC 2 cut(s) 37, 402
BcuI ACTAGT 1 cut(s) 829
BfaI CTAG 3 cut(s) 633, 830, 1049
BfmI CTRYAG 1 cut(s) 687
BisI GCNGC 4 cut(s) 105, 546, 804, 942
BlsI GCNGC 4 cut(s) 106, 547, 805, 943
Bme1390I CCNGG 2 cut(s) 384, 405
Bme18I GGWCC 2 cut(s) 380, 779
BmeT110I CYCGRG 1 cut(s) 549
BmgT120I GGNCC 2 cut(s) 380, 779
BmiI GGNNCC 2 cut(s) 340, 684
BmrFI CCNGG 2 cut(s) 384, 405
BmsI GCATC 2 cut(s) 225, 319
BpiI GAAGAC 1 cut(s) 1029
BpmI CTGGAG 2 cut(s) 664, 807
Bpu14I TTCGAA 1 cut(s) 1111
BpuEI CTTGAG 1 cut(s) 597
BsaHI GRCGYC 2 cut(s) 63, 898
BsaI GGTCTC 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 780, 810
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 556, 617, 1062
Bse118I RCCGGY 1 cut(s) 944
Bse1I ACTGG 4 cut(s) 272, 418, 647, 790
Bse21I CCTNAGG 1 cut(s) 540
Bse3DI GCAATG 1 cut(s) 1088
BseBI CCWGG 2 cut(s) 384, 405
BseGI GGATG 5 cut(s) 217, 240, 369, 1031, 1035
BseLI CCNNNNNNNGG 4 cut(s) 90, 556, 617, 1062
BseMI GCAATG 1 cut(s) 1088
BseMII CTCAG 5 cut(s) 48, 531, 720, 989, 1031
BseNI ACTGG 4 cut(s) 272, 418, 647, 790
BseXI GCAGC 4 cut(s) 91, 557, 790, 928
BseYI CCCAGC 3 cut(s) 522, 668, 876
BsgI GTGCAG 1 cut(s) 591
BshNI GGYRCC 2 cut(s) 338, 682
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 1 cut(s) 945
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 4 cut(s) 90, 556, 617, 1062
BsmAI GTCTC 2 cut(s) 37, 402
BsmFI GGGAC 1 cut(s) 349
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 1 cut(s) 549
Bsp119I TTCGAA 1 cut(s) 1111
Bsp1286I GDGCHC 1 cut(s) 1056
Bsp143I GATC 6 cut(s) 343, 526, 637, 747, 855, 892
BspACI CCGC 3 cut(s) 84, 703, 824
BspCNI CTCAG 5 cut(s) 47, 532, 721, 990, 1030
BspLI GGNNCC 2 cut(s) 340, 684
BspPI GGATC 2 cut(s) 534, 645
BspT104I TTCGAA 1 cut(s) 1111
BspT107I GGYRCC 2 cut(s) 338, 682
BspTNI GGTCTC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 1088
BsrFI RCCGGY 1 cut(s) 944
BsrI ACTGG 4 cut(s) 272, 418, 647, 790
BssAI RCCGGY 1 cut(s) 944
BssMI GATC 6 cut(s) 343, 526, 637, 747, 855, 892
BssNI GRCGYC 2 cut(s) 63, 898
Bst2UI CCWGG 2 cut(s) 384, 405
Bst4CI ACNGT 1 cut(s) 1007
Bst6I CTCTTC 1 cut(s) 282
BstACI GRCGYC 2 cut(s) 63, 898
BstAPI GCANNNNNTGC 1 cut(s) 338
BstBI TTCGAA 1 cut(s) 1111
BstC8I GCNNGC 4 cut(s) 52, 198, 479, 666
BstDEI CTNAG 7 cut(s) 34, 230, 468, 540, 729, 998, 1017
BstF5I GGATG 5 cut(s) 217, 240, 369, 1031, 1035
BstKTI GATC 6 cut(s) 346, 529, 640, 750, 858, 895
BstMAI GTCTC 2 cut(s) 37, 402
BstMBI GATC 6 cut(s) 343, 526, 637, 747, 855, 892
BstMWI GCNNNNNNNGC 6 cut(s) 202, 299, 338, 478, 551, 655
BstNI CCWGG 2 cut(s) 384, 405
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 2 cut(s) 382, 403
BstSFI CTRYAG 1 cut(s) 687
BstV1I GCAGC 4 cut(s) 91, 557, 790, 928
BstV2I GAAGAC 1 cut(s) 1029
Bsu36I CCTNAGG 1 cut(s) 540
BtsCI GGATG 5 cut(s) 217, 240, 369, 1031, 1035
BtsIMutI CAGTG 2 cut(s) 411, 640
Cac8I GCNNGC 4 cut(s) 52, 198, 479, 666
Cfr10I RCCGGY 1 cut(s) 944
Cfr13I GGNCC 2 cut(s) 380, 779
Csp6I GTAC 1 cut(s) 827
CviAII CATG 2 cut(s) 665, 734
CviQI GTAC 1 cut(s) 827
DdeI CTNAG 7 cut(s) 34, 230, 468, 540, 729, 998, 1017
DpnI GATC 6 cut(s) 345, 528, 639, 749, 857, 894
DpnII GATC 6 cut(s) 343, 526, 637, 747, 855, 892
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 1056
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 2 cut(s) 380, 779
Eco57I CTGAAG 1 cut(s) 1062
Eco81I CCTNAGG 1 cut(s) 540
Eco88I CYCGRG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 779
EcoRII CCWGG 2 cut(s) 382, 403
EcoT22I ATGCAT 1 cut(s) 1090
EcoT38I GRGCYC 1 cut(s) 1056
FaeI CATG 2 cut(s) 668, 737
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 1 cut(s) 349
FatI CATG 2 cut(s) 664, 733
Fnu4HI GCNGC 4 cut(s) 105, 546, 804, 942
FokI GGATG 5 cut(s) 204, 247, 356, 1038, 1042
FriOI GRGCYC 1 cut(s) 1056
Fsp4HI GCNGC 4 cut(s) 105, 546, 804, 942
FspBI CTAG 3 cut(s) 633, 830, 1049
GluI GCNGC 4 cut(s) 105, 546, 804, 942
GsaI CCCAGC 3 cut(s) 526, 672, 880
GsuI CTGGAG 2 cut(s) 664, 807
HapII CCGG 1 cut(s) 945
Hin1I GRCGYC 2 cut(s) 63, 898
Hin1II CATG 2 cut(s) 668, 737
HinfI GANTC 3 cut(s) 417, 629, 1066
HpaII CCGG 1 cut(s) 945
HphI GGTGA 2 cut(s) 470, 985
Hpy166II GTNNAC 1 cut(s) 1065
Hpy188I TCNGA 2 cut(s) 607, 1042
Hpy188III TCNNGA 5 cut(s) 430, 539, 614, 902, 1078
Hpy8I GTNNAC 1 cut(s) 1065
Hpy99I CGWCG 5 cut(s) 62, 65, 68, 80, 900
HpyAV CCTTC 2 cut(s) 146, 1107
HpyCH4III ACNGT 1 cut(s) 1007
HpyCH4IV ACGT 3 cut(s) 63, 75, 898
HpyF10VI GCNNNNNNNGC 6 cut(s) 202, 299, 338, 478, 551, 655
HpyF3I CTNAG 7 cut(s) 34, 230, 468, 540, 729, 998, 1017
HpySE526I ACGT 3 cut(s) 63, 75, 898
Hsp92I GRCGYC 2 cut(s) 63, 898
Hsp92II CATG 2 cut(s) 668, 737
Kzo9I GATC 6 cut(s) 343, 526, 637, 747, 855, 892
LmnI GCTCC 1 cut(s) 142
Lsp1109I GCAGC 4 cut(s) 91, 557, 790, 928
LweI GCATC 2 cut(s) 225, 319
MaeI CTAG 3 cut(s) 633, 830, 1049
MaeII ACGT 3 cut(s) 63, 75, 898
MaeIII GTNAC 5 cut(s) 691, 724, 761, 796, 1001
MalI GATC 6 cut(s) 345, 528, 639, 749, 857, 894
MboI GATC 6 cut(s) 343, 526, 637, 747, 855, 892
MboII GAAGA 4 cut(s) 299, 554, 988, 1029
MfeI CAATTG 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 1056
MluCI AATT 9 cut(s) 310, 315, 482, 507, 516, 676, 720, 792, 839
MlyI GAGTC 1 cut(s) 1060
MmeI TCCRAC 2 cut(s) 245, 1018
Mph1103I ATGCAT 1 cut(s) 1090
MseI TTAA 2 cut(s) 506, 1083
MspA1I CMGCKG 1 cut(s) 803
MspI CCGG 1 cut(s) 945
MspR9I CCNGG 2 cut(s) 384, 405
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 2 cut(s) 384, 405
MwoI GCNNNNNNNGC 6 cut(s) 202, 299, 338, 478, 551, 655
NdeII GATC 6 cut(s) 343, 526, 637, 747, 855, 892
NlaIII CATG 2 cut(s) 668, 737
NlaIV GGNNCC 2 cut(s) 340, 684
NmuCI GTSAC 1 cut(s) 724
NsiI ATGCAT 1 cut(s) 1090
NspI RCATGY 1 cut(s) 668
NspV TTCGAA 1 cut(s) 1111
PaeI GCATGC 1 cut(s) 668
PcsI WCGNNNNNNNCGW 3 cut(s) 54, 72, 84
PfeI GAWTC 2 cut(s) 417, 629
PflFI GACNNNGTC 1 cut(s) 62
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 4 cut(s) 106, 547, 805, 943
PleI GAGTC 1 cut(s) 1060
PpsI GAGTC 1 cut(s) 1060
PpuMI RGGWCCY 1 cut(s) 779
PshBI ATTAAT 1 cut(s) 1083
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 2 cut(s) 382, 403
PspFI CCCAGC 3 cut(s) 522, 668, 876
PspGI CCWGG 2 cut(s) 382, 403
PspN4I GGNNCC 2 cut(s) 340, 684
PspPI GGNCC 2 cut(s) 380, 779
PspPPI RGGWCCY 1 cut(s) 779
PsyI GACNNNGTC 1 cut(s) 62
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 1 cut(s) 828
RsaNI GTAC 1 cut(s) 827
SaqAI TTAA 2 cut(s) 506, 1083
SatI GCNGC 4 cut(s) 105, 546, 804, 942
Sau3AI GATC 6 cut(s) 343, 526, 637, 747, 855, 892
Sau96I GGNCC 2 cut(s) 380, 779
SchI GAGTC 1 cut(s) 1060
ScrFI CCNGG 2 cut(s) 384, 405
SduI GDGCHC 1 cut(s) 1056
SfaNI GCATC 2 cut(s) 225, 319
SfcI CTRYAG 1 cut(s) 687
SfuI TTCGAA 1 cut(s) 1111
SinI GGWCC 2 cut(s) 380, 779
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
SpeI ACTAGT 1 cut(s) 829
SphI GCATGC 1 cut(s) 668
Sse9I AATT 9 cut(s) 310, 315, 482, 507, 516, 676, 720, 792, 839
SsiI CCGC 3 cut(s) 84, 703, 824
SspI AATATT 2 cut(s) 926, 990
SspMI CTAG 3 cut(s) 633, 830, 1049
StyD4I CCNGG 2 cut(s) 382, 403
TaaI ACNGT 1 cut(s) 1007
TaiI ACGT 3 cut(s) 66, 78, 901
TaqI TCGA 2 cut(s) 895, 1111
TasI AATT 9 cut(s) 310, 315, 482, 507, 516, 676, 720, 792, 839
TfiI GAWTC 2 cut(s) 417, 629
Tru1I TTAA 2 cut(s) 506, 1083
Tru9I TTAA 2 cut(s) 506, 1083
TscAI CASTG 2 cut(s) 418, 647
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 4 cut(s) 104, 545, 803, 941
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 3 cut(s) 178, 760, 834
TspGWI ACGGA 1 cut(s) 878
TspRI CASTG 2 cut(s) 418, 647
Tth111I GACNNNGTC 1 cut(s) 62
VpaK11BI GGWCC 2 cut(s) 380, 779
VspI ATTAAT 1 cut(s) 1083
XapI RAATTY 2 cut(s) 676, 839
XceI RCATGY 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XspI CTAG 3 cut(s) 633, 830, 1049
ZraI GACGTC 2 cut(s) 64, 899
Zsp2I ATGCAT 1 cut(s) 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.