MD03G1147500.v1.1

N-carbamoylputrescine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006306
Physical Location & Seq
Forward (+)
16252492 .. 16257118
4627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1147500.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGAGAAGGGCAAGAGGGAGGTGGTGGTCTCAGCTCTGCAGTTCGCTTGCACCGACGACGTCGCCACCAACGTCGCCACCGCCGAAAGGTTGGTTAGAGCTGCCCATGCGAAGGGTGCAAACATCATTCTTATACAGGAACTTTTTGAGGGACATTACTTCTGTCAGGCACAAAGGGAGGACTTCTTTCAGCGAGCAAAGCCTTACAAGGATCATCCAACCATTCTTAGGATGCAAATACTTGCAAAAGAGTTGGGTGTGGTAATACCAGTTAGCTTTTTCGAAGAGGCAAATAATGCTCATTACAATTCAGTTGCCATTATAGATGCTGATGGGGCAGATCTTGGACTTTATAGGAAGTCCCACATCCCAGACGGACCTGGCTACCAGGAAAAGTTCTACTTCAATCCAGGTGACACTGGATTCAAGGTTTTCAAGACCAAGTTCGCAACCATTGGAGTTGCAATTTGCTGGGATCAGTGGTTCCCTGAGGCTGCCCGGTGTTTGGTTCTTCAAGGTGCAGAGATATTGTTCTACCCAACTGCTATTGGTTCCGAACCTCAAGATGGAGGACTTGATTCTCGGGATCACTGGAGGCGAGTGATGCAAGGGCATGCTGGGGCTAATGTGGTCCCTGTAGTAACTTCAAATCGTATAGGAAAGGAGATAATCGAGACCGAGCACGGAAAGAGTGAGATCACTTTCTATGGTAACTCTTTCATAGCAGGACCCACTGGAGAAATTGTTGCAACTGCTAATGATAAGGAGGAAGCTGTACTAGTAGCACAATTTGACCTAGACAAAATCAAATGGAAGAGACATAGCTGGGGAGTATTCCGTGATCGCCGCCCAGATTTATACAAGGTGCTACTGACATCAGATGGCAGCAATTCCCCGTTGTGA

Protein Analysis

301

Amino Acids

33.48

Weight (kDa)

6.01

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 12 - 269 3e-53 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
AciI CCGC 2 cut(s) 81, 847
AclWI GGATC 3 cut(s) 219, 483, 594
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 777
AfiI CCNNNNNNNGG 5 cut(s) 87, 112, 228, 505, 566
AgsI TTSAA 5 cut(s) 406, 427, 436, 515, 648
AhlI ACTAGT 1 cut(s) 778
AjnI CCWGG 3 cut(s) 379, 387, 409
AloI GAACNNNNNNTCC 2 cut(s) 467, 499
AluBI AGCT 5 cut(s) 35, 101, 276, 773, 825
AluI AGCT 5 cut(s) 35, 101, 276, 773, 825
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 3 cut(s) 34, 668, 811
AlwI GGATC 3 cut(s) 219, 483, 594
Ama87I CYCGRG 1 cut(s) 582
ApeKI GCWGC 3 cut(s) 101, 494, 885
AspS9I GGNCC 3 cut(s) 377, 631, 728
AsuC2I CCSGG 1 cut(s) 499
AsuHPI GGTGA 1 cut(s) 425
AsuII TTCGAA 1 cut(s) 282
AvaI CYCGRG 1 cut(s) 582
AvaII GGWCC 3 cut(s) 377, 631, 728
AxyI CCTNAGG 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 3 cut(s) 88, 481, 897
BccI CCATC 3 cut(s) 326, 560, 875
BciT130I CCWGG 3 cut(s) 381, 389, 411
BcnI CCSGG 1 cut(s) 499
BcoDI GTCTC 3 cut(s) 34, 668, 811
BcuI ACTAGT 1 cut(s) 778
BfaI CTAG 2 cut(s) 779, 797
BfmI CTRYAG 2 cut(s) 38, 636
BglII AGATCT 1 cut(s) 340
BisI GCNGC 4 cut(s) 102, 495, 847, 886
BlsI GCNGC 4 cut(s) 103, 496, 848, 887
Bme1390I CCNGG 4 cut(s) 381, 389, 411, 499
Bme18I GGWCC 3 cut(s) 377, 631, 728
BmeT110I CYCGRG 1 cut(s) 582
BmgT120I GGNCC 3 cut(s) 377, 631, 728
BmiI GGNNCC 4 cut(s) 485, 553, 633, 730
BmrFI CCNGG 4 cut(s) 381, 389, 411, 499
BmsI GCATC 3 cut(s) 222, 316, 594
BpmI CTGGAG 2 cut(s) 613, 756
Bpu14I TTCGAA 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 499
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 2 cut(s) 34, 668
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 112, 228, 505, 566
Bse1I ACTGG 4 cut(s) 269, 424, 596, 739
Bse21I CCTNAGG 1 cut(s) 489
BseBI CCWGG 3 cut(s) 381, 389, 411
BseGI GGATG 3 cut(s) 214, 237, 366
BseLI CCNNNNNNNGG 5 cut(s) 87, 112, 228, 505, 566
BseMII CTCAG 2 cut(s) 45, 480
BseNI ACTGG 4 cut(s) 269, 424, 596, 739
BseXI GCAGC 3 cut(s) 88, 481, 897
BseYI CCCAGC 3 cut(s) 471, 617, 825
BsgI GTGCAG 1 cut(s) 540
BsiHKAI GWGCWC 1 cut(s) 684
BsiHKCI CYCGRG 1 cut(s) 582
BsiSI CCGG 1 cut(s) 499
BslFI GGGAC 3 cut(s) 165, 346, 617
BslI CCNNNNNNNGG 5 cut(s) 87, 112, 228, 505, 566
BsmAI GTCTC 3 cut(s) 34, 668, 811
BsmFI GGGAC 3 cut(s) 165, 346, 617
Bso31I GGTCTC 2 cut(s) 34, 668
BsoBI CYCGRG 1 cut(s) 582
Bsp119I TTCGAA 1 cut(s) 282
Bsp1286I GDGCHC 1 cut(s) 684
Bsp143I GATC 6 cut(s) 211, 340, 475, 586, 696, 841
BspACI CCGC 2 cut(s) 81, 847
BspCNI CTCAG 2 cut(s) 44, 481
BspLI GGNNCC 4 cut(s) 485, 553, 633, 730
BspMAI CTGCAG 1 cut(s) 42
BspPI GGATC 3 cut(s) 219, 483, 594
BspT104I TTCGAA 1 cut(s) 282
BspTNI GGTCTC 2 cut(s) 34, 668
BsrI ACTGG 4 cut(s) 269, 424, 596, 739
BssMI GATC 6 cut(s) 211, 340, 475, 586, 696, 841
BssNI GRCGYC 1 cut(s) 60
Bst2UI CCWGG 3 cut(s) 381, 389, 411
Bst6I CTCTTC 2 cut(s) 279, 809
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 3 cut(s) 49, 195, 615
BstDEI CTNAG 3 cut(s) 31, 227, 489
BstF5I GGATG 3 cut(s) 214, 237, 366
BstKTI GATC 6 cut(s) 214, 343, 478, 589, 699, 844
BstMAI GTCTC 3 cut(s) 34, 668, 811
BstMBI GATC 6 cut(s) 211, 340, 475, 586, 696, 841
BstMWI GCNNNNNNNGC 6 cut(s) 107, 116, 199, 296, 335, 604
BstNI CCWGG 3 cut(s) 381, 389, 411
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 4 cut(s) 379, 387, 409, 497
BstSFI CTRYAG 2 cut(s) 38, 636
BstV1I GCAGC 3 cut(s) 88, 481, 897
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
Bsu36I CCTNAGG 1 cut(s) 489
BtsCI GGATG 3 cut(s) 214, 237, 366
BtsIMutI CAGTG 4 cut(s) 417, 485, 589, 732
Cac8I GCNNGC 3 cut(s) 49, 195, 615
Cfr13I GGNCC 3 cut(s) 377, 631, 728
Csp6I GTAC 1 cut(s) 776
CviAII CATG 2 cut(s) 107, 614
CviJI RGCY 9 cut(s) 35, 101, 202, 276, 384, 494, 623, 773, 825
CviKI_1 RGCY 9 cut(s) 35, 101, 202, 276, 384, 494, 623, 773, 825
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 3 cut(s) 31, 227, 489
DpnI GATC 6 cut(s) 213, 342, 477, 588, 698, 843
DpnII GATC 6 cut(s) 211, 340, 475, 586, 696, 841
Eam1104I CTCTTC 2 cut(s) 279, 809
EarI CTCTTC 2 cut(s) 279, 809
Eco31I GGTCTC 2 cut(s) 34, 668
Eco47I GGWCC 3 cut(s) 377, 631, 728
Eco81I CCTNAGG 1 cut(s) 489
Eco88I CYCGRG 1 cut(s) 582
EcoO109I RGGNCCY 1 cut(s) 728
EcoRII CCWGG 3 cut(s) 379, 387, 409
FaeI CATG 2 cut(s) 110, 617
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FaqI GGGAC 3 cut(s) 165, 346, 617
FatI CATG 2 cut(s) 106, 613
Fnu4HI GCNGC 4 cut(s) 102, 495, 847, 886
FokI GGATG 3 cut(s) 201, 244, 353
Fsp4HI GCNGC 4 cut(s) 102, 495, 847, 886
FspBI CTAG 2 cut(s) 779, 797
GluI GCNGC 4 cut(s) 102, 495, 847, 886
GsaI CCCAGC 3 cut(s) 475, 621, 829
GsuI CTGGAG 2 cut(s) 613, 756
HapII CCGG 1 cut(s) 499
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 2 cut(s) 110, 617
HinfI GANTC 2 cut(s) 423, 578
HpaII CCGG 1 cut(s) 499
HphI GGTGA 1 cut(s) 425
Hpy188I TCNGA 2 cut(s) 556, 880
Hpy188III TCNNGA 4 cut(s) 436, 563, 584, 673
Hpy99I CGWCG 4 cut(s) 59, 62, 65, 77
HpyAV CCTTC 1 cut(s) 106
HpyCH4IV ACGT 2 cut(s) 60, 72
HpyCH4V TGCA 9 cut(s) 40, 51, 119, 235, 245, 464, 521, 607, 749
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 116, 199, 296, 335, 604
HpyF3I CTNAG 3 cut(s) 31, 227, 489
HpySE526I ACGT 2 cut(s) 60, 72
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 2 cut(s) 110, 617
Kzo9I GATC 6 cut(s) 211, 340, 475, 586, 696, 841
Lsp1109I GCAGC 3 cut(s) 88, 481, 897
LweI GCATC 3 cut(s) 222, 316, 594
MaeI CTAG 2 cut(s) 779, 797
MaeII ACGT 2 cut(s) 60, 72
MaeIII GTNAC 3 cut(s) 413, 640, 710
MalI GATC 6 cut(s) 213, 342, 477, 588, 698, 843
MboI GATC 6 cut(s) 211, 340, 475, 586, 696, 841
MboII GAAGA 3 cut(s) 296, 503, 826
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 1 cut(s) 684
MluCI AATT 5 cut(s) 307, 465, 741, 788, 889
MmeI TCCRAC 1 cut(s) 242
MspI CCGG 1 cut(s) 499
MspR9I CCNGG 4 cut(s) 381, 389, 411, 499
MvaI CCWGG 3 cut(s) 381, 389, 411
MwoI GCNNNNNNNGC 6 cut(s) 107, 116, 199, 296, 335, 604
NciI CCSGG 1 cut(s) 499
NdeII GATC 6 cut(s) 211, 340, 475, 586, 696, 841
NlaIII CATG 2 cut(s) 110, 617
NlaIV GGNNCC 4 cut(s) 485, 553, 633, 730
NmuCI GTSAC 1 cut(s) 413
NspI RCATGY 1 cut(s) 617
NspV TTCGAA 1 cut(s) 282
PaeI GCATGC 1 cut(s) 617
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 69, 81
PfeI GAWTC 2 cut(s) 423, 578
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 4 cut(s) 103, 496, 848, 887
PpuMI RGGWCCY 1 cut(s) 728
Psp5II RGGWCCY 1 cut(s) 728
Psp6I CCWGG 3 cut(s) 379, 387, 409
PspFI CCCAGC 3 cut(s) 471, 617, 825
PspGI CCWGG 3 cut(s) 379, 387, 409
PspN4I GGNNCC 4 cut(s) 485, 553, 633, 730
PspPI GGNCC 3 cut(s) 377, 631, 728
PspPPI RGGWCCY 1 cut(s) 728
PstI CTGCAG 1 cut(s) 42
PsuI RGATCY 1 cut(s) 340
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
SatI GCNGC 4 cut(s) 102, 495, 847, 886
Sau3AI GATC 6 cut(s) 211, 340, 475, 586, 696, 841
Sau96I GGNCC 3 cut(s) 377, 631, 728
ScrFI CCNGG 4 cut(s) 381, 389, 411, 499
SduI GDGCHC 1 cut(s) 684
SfaNI GCATC 3 cut(s) 222, 316, 594
SfcI CTRYAG 2 cut(s) 38, 636
SfuI TTCGAA 1 cut(s) 282
SinI GGWCC 3 cut(s) 377, 631, 728
SmlI CTYRAG 1 cut(s) 561
SmoI CTYRAG 1 cut(s) 561
SpeI ACTAGT 1 cut(s) 778
SphI GCATGC 1 cut(s) 617
Sse9I AATT 5 cut(s) 307, 465, 741, 788, 889
SsiI CCGC 2 cut(s) 81, 847
SspMI CTAG 2 cut(s) 779, 797
StyD4I CCNGG 4 cut(s) 379, 387, 409, 497
TaiI ACGT 2 cut(s) 63, 75
TaqI TCGA 2 cut(s) 282, 672
TaqII GACCGA 1 cut(s) 692
TasI AATT 5 cut(s) 307, 465, 741, 788, 889
TatI WGTACW 1 cut(s) 775
TauI GCSGC 1 cut(s) 849
TfiI GAWTC 2 cut(s) 423, 578
TscAI CASTG 4 cut(s) 424, 485, 596, 739
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 3 cut(s) 101, 494, 885
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 709
TspGWI ACGGA 3 cut(s) 390, 699, 827
TspRI CASTG 4 cut(s) 424, 485, 596, 739
Tth111I GACNNNGTC 1 cut(s) 59
VpaK11BI GGWCC 3 cut(s) 377, 631, 728
XceI RCATGY 1 cut(s) 617
XspI CTAG 2 cut(s) 779, 797
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.