MD11G1166200.v1.1

N-carbamoylputrescine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006306
Physical Location & Seq
Forward (+)
17587121 .. 17591331
4211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1166200.v1.1.491

Sequence Viewer

Length: 891 bp
ATGGAAAAGGATAAACGGGAGGTGGTGGTTTCAGCTCTGCAATTCGCTTGCACCGACGACGTCGCCACCAACGTCGCCACCGCCGAAAGGTTGGTTAGAGCTGCTCATGCGAAGGGTGCAAATATCATTCTTATACAGGAACTCTTTGAGGGACATTACTTCTGTCAGGCACAAAGGGAGGACTTCTTTCAGATGCAGAAACTTGCCAAAGAGTTGGGTGTGGTAATACCAGTTAGCTTTTTCGAAGAGGCAAATAATGCCCATTACAATTCAATTGCCATTATTGATGCTGATGGGGCAGATCTTGGACTTTATAGGAAGTCCCACATCCCAGACGGACCAGGTATTCGAGCTACCAGGAAAAGTTCTACTTCAATCCAAGGTGATACTGGATTCAAGGTTTTCAAGACCAAGTTTGCAACTATTGCAGTTGCAATTTGCTGGGATCAATGGTTTCCCGAGGCTGCCCGGGCTTTGGAGGCTGCCCGGGCTTTGGTTCTTCAAGGTGCTGAGATATTGTTTTACCCCACTGCTATTGGTTCCGAACCTCAAGATGGAGGTCTTGATTCTCGTGATCACTGGAGGCGAGTGATGCAAGGGCATGCTGGGGCTAATGTGGTCCCTGTAGTAACTTCAAATCGCATAGGAAAGGAGATAATTGAGACCGAGCATGGAAAGAGTGAGATCACTTTCTACGGTAACTCTTTTATAGCAGGACCCACTGGTGAAATTGTTGCAACTGCTAATGATAAAGAGGAAGCTGTGCTTGTAGCCCAGTTTGACCTAGACAAAATCAAATGGAAGAGACATAGCTGGGGAGTATTCCGCGACCGCCGCCCAGATTTATACAAGGTGCTACTGACATCAGATGGCAGCAATTCCCCTTTGTGA

Protein Analysis

297

Amino Acids

32.7

Weight (kDa)

5.94

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 12 - 265 9.7e-44 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
AccII CGCG 1 cut(s) 828
AciI CCGC 4 cut(s) 81, 826, 832, 835
AclWI GGATC 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 60
AfiI CCNNNNNNNGG 4 cut(s) 87, 475, 493, 554
AgsI TTSAA 6 cut(s) 273, 375, 397, 406, 503, 636
AjnI CCWGG 2 cut(s) 340, 356
AluBI AGCT 6 cut(s) 35, 101, 237, 353, 761, 813
AluI AGCT 6 cut(s) 35, 101, 237, 353, 761, 813
Alw26I GTCTC 2 cut(s) 656, 799
AlwI GGATC 1 cut(s) 453
Ama87I CYCGRG 3 cut(s) 458, 468, 486
ApeKI GCWGC 4 cut(s) 101, 464, 482, 873
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
AspS9I GGNCC 3 cut(s) 338, 619, 716
AsuC2I CCSGG 4 cut(s) 469, 470, 487, 488
AsuHPI GGTGA 2 cut(s) 395, 737
AsuII TTCGAA 1 cut(s) 243
AvaI CYCGRG 3 cut(s) 458, 468, 486
AvaII GGWCC 3 cut(s) 338, 619, 716
BauI CACGAG 1 cut(s) 570
BbvI GCAGC 4 cut(s) 88, 451, 469, 885
BccI CCATC 3 cut(s) 287, 548, 863
BciT130I CCWGG 2 cut(s) 342, 358
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 4 cut(s) 469, 470, 487, 488
BcoDI GTCTC 2 cut(s) 656, 799
BfaI CTAG 1 cut(s) 785
BfmI CTRYAG 1 cut(s) 624
BglII AGATCT 1 cut(s) 301
BisI GCNGC 5 cut(s) 102, 465, 483, 835, 874
BlsI GCNGC 5 cut(s) 103, 466, 484, 836, 875
Bme1390I CCNGG 6 cut(s) 342, 358, 469, 470, 487, 488
Bme18I GGWCC 3 cut(s) 338, 619, 716
BmeT110I CYCGRG 3 cut(s) 458, 468, 486
BmgT120I GGNCC 3 cut(s) 338, 619, 716
BmiI GGNNCC 3 cut(s) 541, 621, 718
BmrFI CCNGG 6 cut(s) 342, 358, 469, 470, 487, 488
BmrI ACTGGG 1 cut(s) 769
BmsI GCATC 3 cut(s) 183, 277, 582
BmuI ACTGGG 1 cut(s) 769
BpmI CTGGAG 1 cut(s) 601
Bpu14I TTCGAA 1 cut(s) 243
BpuEI CTTGAG 1 cut(s) 534
BpuMI CCSGG 4 cut(s) 469, 470, 487, 488
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 4 cut(s) 379, 459, 468, 486
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 475, 493, 554
Bse1I ACTGG 5 cut(s) 230, 394, 584, 727, 775
BseBI CCWGG 2 cut(s) 342, 358
BseDI CCNNGG 4 cut(s) 379, 459, 468, 486
BseGI GGATG 1 cut(s) 327
BseLI CCNNNNNNNGG 4 cut(s) 87, 475, 493, 554
BseMII CTCAG 1 cut(s) 501
BseNI ACTGG 5 cut(s) 230, 394, 584, 727, 775
BseXI GCAGC 4 cut(s) 88, 451, 469, 885
BseYI CCCAGC 3 cut(s) 441, 605, 813
Bsh1236I CGCG 1 cut(s) 828
Bsh1285I CGRYCG 1 cut(s) 832
BsiEI CGRYCG 1 cut(s) 832
BsiHKCI CYCGRG 3 cut(s) 458, 468, 486
BsiSI CCGG 2 cut(s) 469, 487
BslFI GGGAC 3 cut(s) 165, 307, 605
BslI CCNNNNNNNGG 4 cut(s) 87, 475, 493, 554
BsmAI GTCTC 2 cut(s) 656, 799
BsmFI GGGAC 3 cut(s) 165, 307, 605
Bso31I GGTCTC 1 cut(s) 656
BsoBI CYCGRG 3 cut(s) 458, 468, 486
Bsp119I TTCGAA 1 cut(s) 243
Bsp143I GATC 4 cut(s) 301, 445, 574, 684
BspACI CCGC 4 cut(s) 81, 826, 832, 835
BspCNI CTCAG 1 cut(s) 502
BspFNI CGCG 1 cut(s) 828
BspLI GGNNCC 3 cut(s) 541, 621, 718
BspPI GGATC 1 cut(s) 453
BspT104I TTCGAA 1 cut(s) 243
BspTNI GGTCTC 1 cut(s) 656
BsrI ACTGG 5 cut(s) 230, 394, 584, 727, 775
BssECI CCNNGG 4 cut(s) 379, 459, 468, 486
BssMI GATC 4 cut(s) 301, 445, 574, 684
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 379
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 2 cut(s) 342, 358
Bst4CI ACNGT 1 cut(s) 698
Bst6I CTCTTC 2 cut(s) 240, 797
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 2 cut(s) 257, 425
BstBI TTCGAA 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 49, 603
BstDEI CTNAG 1 cut(s) 510
BstF5I GGATG 1 cut(s) 327
BstFNI CGCG 1 cut(s) 828
BstKTI GATC 4 cut(s) 304, 448, 577, 687
BstMAI GTCTC 2 cut(s) 656, 799
BstMBI GATC 4 cut(s) 301, 445, 574, 684
BstMCI CGRYCG 1 cut(s) 832
BstNI CCWGG 2 cut(s) 342, 358
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 6 cut(s) 340, 356, 467, 468, 485, 486
BstSFI CTRYAG 1 cut(s) 624
BstUI CGCG 1 cut(s) 828
BstV1I GCAGC 4 cut(s) 88, 451, 469, 885
BstX2I RGATCY 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 214
BstYI RGATCY 1 cut(s) 301
BtsCI GGATG 1 cut(s) 327
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 3 cut(s) 528, 577, 720
Cac8I GCNNGC 2 cut(s) 49, 603
Cfr13I GGNCC 3 cut(s) 338, 619, 716
Cfr9I CCCGGG 2 cut(s) 468, 486
CsiI ACCWGGT 1 cut(s) 340
CspCI CAANNNNNGTGG 2 cut(s) 517, 552
CviAII CATG 3 cut(s) 107, 602, 671
DdeI CTNAG 1 cut(s) 510
DpnI GATC 4 cut(s) 303, 447, 576, 686
DpnII GATC 4 cut(s) 301, 445, 574, 684
Eam1104I CTCTTC 2 cut(s) 240, 797
EarI CTCTTC 2 cut(s) 240, 797
Eco130I CCWWGG 1 cut(s) 379
Eco31I GGTCTC 1 cut(s) 656
Eco47I GGWCC 3 cut(s) 338, 619, 716
Eco88I CYCGRG 3 cut(s) 458, 468, 486
EcoO109I RGGNCCY 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 340, 356
EcoT14I CCWWGG 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 379
FaeI CATG 3 cut(s) 110, 605, 674
FaiI YATR 9 cut(s) 108, 134, 315, 603, 644, 672, 710, 810, 847
FalI AAGNNNNNCTT 4 cut(s) 355, 387, 750, 782
FaqI GGGAC 3 cut(s) 165, 307, 605
FatI CATG 3 cut(s) 106, 601, 670
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 5 cut(s) 102, 465, 483, 835, 874
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 5 cut(s) 102, 465, 483, 835, 874
FspBI CTAG 1 cut(s) 785
GluI GCNGC 5 cut(s) 102, 465, 483, 835, 874
GsaI CCCAGC 3 cut(s) 445, 609, 817
GsuI CTGGAG 1 cut(s) 601
HapII CCGG 2 cut(s) 469, 487
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 3 cut(s) 110, 605, 674
HinfI GANTC 2 cut(s) 393, 566
HpaII CCGG 2 cut(s) 469, 487
HphI GGTGA 2 cut(s) 395, 737
Hpy188I TCNGA 3 cut(s) 192, 544, 868
Hpy188III TCNNGA 5 cut(s) 406, 458, 551, 563, 572
Hpy99I CGWCG 4 cut(s) 59, 62, 65, 77
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 698
HpyCH4IV ACGT 2 cut(s) 60, 72
HpyCH4V TGCA 9 cut(s) 40, 51, 119, 196, 419, 428, 434, 595, 737
HpyF3I CTNAG 1 cut(s) 510
HpySE526I ACGT 2 cut(s) 60, 72
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 3 cut(s) 110, 605, 674
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 4 cut(s) 301, 445, 574, 684
Lsp1109I GCAGC 4 cut(s) 88, 451, 469, 885
LweI GCATC 3 cut(s) 183, 277, 582
MabI ACCWGGT 1 cut(s) 340
MaeI CTAG 1 cut(s) 785
MaeII ACGT 2 cut(s) 60, 72
MaeIII GTNAC 2 cut(s) 628, 698
MalI GATC 4 cut(s) 303, 447, 576, 686
MboI GATC 4 cut(s) 301, 445, 574, 684
MboII GAAGA 3 cut(s) 257, 491, 814
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 1 cut(s) 301
MluCI AATT 7 cut(s) 41, 268, 273, 435, 657, 729, 877
MspI CCGG 2 cut(s) 469, 487
MspR9I CCNGG 6 cut(s) 342, 358, 469, 470, 487, 488
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 2 cut(s) 342, 358
MvnI CGCG 1 cut(s) 828
NciI CCSGG 4 cut(s) 469, 470, 487, 488
NdeII GATC 4 cut(s) 301, 445, 574, 684
NlaIII CATG 3 cut(s) 110, 605, 674
NlaIV GGNNCC 3 cut(s) 541, 621, 718
NspI RCATGY 1 cut(s) 605
NspV TTCGAA 1 cut(s) 243
PaeI GCATGC 1 cut(s) 605
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 69, 81
PfeI GAWTC 2 cut(s) 393, 566
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 5 cut(s) 103, 466, 484, 836, 875
PpuMI RGGWCCY 1 cut(s) 716
Psp5II RGGWCCY 1 cut(s) 716
Psp6I CCWGG 2 cut(s) 340, 356
PspFI CCCAGC 3 cut(s) 441, 605, 813
PspGI CCWGG 2 cut(s) 340, 356
PspN4I GGNNCC 3 cut(s) 541, 621, 718
PspPI GGNCC 3 cut(s) 338, 619, 716
PspPPI RGGWCCY 1 cut(s) 716
PsuI RGATCY 1 cut(s) 301
PsyI GACNNNGTC 1 cut(s) 59
SatI GCNGC 5 cut(s) 102, 465, 483, 835, 874
Sau3AI GATC 4 cut(s) 301, 445, 574, 684
Sau96I GGNCC 3 cut(s) 338, 619, 716
ScrFI CCNGG 6 cut(s) 342, 358, 469, 470, 487, 488
SexAI ACCWGGT 1 cut(s) 340
SfaNI GCATC 3 cut(s) 183, 277, 582
SfcI CTRYAG 1 cut(s) 624
SfuI TTCGAA 1 cut(s) 243
SinI GGWCC 3 cut(s) 338, 619, 716
SmaI CCCGGG 2 cut(s) 470, 488
SmlI CTYRAG 1 cut(s) 549
SmoI CTYRAG 1 cut(s) 549
SphI GCATGC 1 cut(s) 605
SrfI GCCCGGGC 2 cut(s) 470, 488
Sse9I AATT 7 cut(s) 41, 268, 273, 435, 657, 729, 877
SsiI CCGC 4 cut(s) 81, 826, 832, 835
SspMI CTAG 1 cut(s) 785
StyD4I CCNGG 6 cut(s) 340, 356, 467, 468, 485, 486
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 1 cut(s) 698
TaiI ACGT 2 cut(s) 63, 75
TaqI TCGA 2 cut(s) 243, 349
TaqII GACCGA 1 cut(s) 680
TasI AATT 7 cut(s) 41, 268, 273, 435, 657, 729, 877
TauI GCSGC 1 cut(s) 837
TfiI GAWTC 2 cut(s) 393, 566
TscAI CASTG 3 cut(s) 535, 584, 727
TseI GCWGC 4 cut(s) 101, 464, 482, 873
TspGWI ACGGA 1 cut(s) 351
TspMI CCCGGG 2 cut(s) 468, 486
TspRI CASTG 3 cut(s) 535, 584, 727
Tth111I GACNNNGTC 1 cut(s) 59
VpaK11BI GGWCC 3 cut(s) 338, 619, 716
XceI RCATGY 1 cut(s) 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 386
XmaI CCCGGG 2 cut(s) 468, 486
XspI CTAG 1 cut(s) 785
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.