AT2G27460

sec23 sec24 transport family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011378
Physical Location & Seq
Forward (+)
11740502 .. 11745011
4510 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27460.1

Sequence Viewer

Length: 2238 bp
ATGGCAGTGAGAGCAACGGTGTCGCGTTTTCCGATCGATTCAGACGCTCAAGAGGCGTCAGGACTTCCTTGGGGACTGACAGTAACACCATTCGCGGCCAAAGACGAGAACGGAATCGGACCAGCGTGCGGATCTAACGGTCATCTTCTTCCTCGTTGCGAAAACTGCTACGCTTACTTCAATACCTATTGTGAGCTTGATCAATGGGCTTGGAATTGCTCGCTTTGTGGAACTCTCAATGGACTTCCTTCTGATGCTATTGCTCGTTACTCTAATCCTCACTCTATACCTGAAATGACTTCCTCCTTCATTGATCTTGAGATGCCTCTGGATGGATCGGAGGAAGAGATGACGCAAGCACGACCTGTGTATGTTGCGGCGATTGATATCTCTTCTTCTGAGGAATTTTTGGAGCTTACAAAAAGTGCATTGCTAGCCGCTCTGGAAGCTCTAAGTCCAGGGGCTCTTTTTGGTCTTGTTACGTTCAGTCACAAAATAGGACTGTATGATGTTCAAGGACCTATACCAGTTGTAAAGAATGTATTCATTCCCCCTGATGGGGAGAGCAGCTTATCGCTAGAACTTGAGGATGTCATGCCCTTGTTACAATTTTTGGCTCCGGTAGAAACTTGCAAGGATCGTATTGCTGCTGCACTTGAGACACTCAGACCAATTACTTCTTGGGAGAGATCAGCTGGAGCAGGTCAAGGGATGGATAGTGTGTTGATGGGTGGTAGGGGCTTTGGTACAGCTATGGAAGCTCTATTCAACTATCTAGGATCTGAATTTGGGAATACATTTGCATTAGCTAGGGTGTTTGCTTTCCTATCTGGTCCTCCTGATTATGGACGTGGACAATTGGATACAAGTAGATATGGTGAACAATATGCAAGTAAAAGGGTAGATGCTGATCGTGCTCTACTTCCTGAACAAACACCATTCTACAAAGACCTAGCCACCATTGCTGTCCAATCAGGTGTATGTGTAGATTTATTTGCAGTTACAAATGAGTACACAGATTTAGCATCCCTGAAGTTCCTTAGCATTGAAAGTGGCGGTTCACTGTTTTTGTATTCAAGCACTGATGATTCAACTCTTCCCCAAGACATGTTTCGAATGCTTAACCGGCCGTATGCATTCAATTGTGTCTTGAGATTGAGGACGTCAACTGAATTCAAACCTGGCAACTCTTTTGGTCACTTCTTTCCAGATCCACAGTACGAGAACCTTCAGCATATCATCTGCTGTGATTCATATGCAACATATGCATATGACTTTGAATTTGCAGATAACACTGGATTTTCCAGGCATTCTGGAGAGCAACCAGTGGTGCAAATTGCATTTCAGTACACAGTTGTTGTACCTCCCGAGGGACTGTCAAATTCAGAAATGTCATCTTCAAGTAGAGGCAAGCACACACTTCAGAGACGATTAAGGATCAGAACCATGCAATTTGGAACTGCCCACAATATCAATGAAATTTACGACAGTGTGGATCATGAAGTTGTTCTCTCATTGCTCGTTCACAAGGTAATTTTAGCCTCTCTGGAAGATGGAGTCAGAGAAGGGAGGGCATTGCTGCATGATTGGCTAGTAATTCTAACAGCACAATACAACGATGCCTTTAACCTTGTCCAATACAAAAACGGAAATAAGTCAATGAGTTCTCAGATTGATATCACGTTTTCACAATGTCCTCAACTTGAGCCTTTGCCTCGTTTAGTCTTTGCCTTACTCCGTAATCCCCTTCTCCGGTTTCATGAAGAAGGTGTTCATCCTGATTACAGAATCTACTTGCAATGCCTTTTCAGTGTGTTGGACCCGAGCTCTCTTCATTGCGGCATTTACCCAGCACTAATGTCATATTCAACACCGGATACACTGGCATATCCGCGTCATTCGTTGAGCCGTGCAGCATTGATAACAAGTGGTAGTCCGATATTTTTCCTAGATGCATATACCACTTTGATAGTCTTCTACTCATCAACAGCAGACCCGTCAATACCTTTCCCTCCACCTCAAGACTGTTTATTGAGGCAGACCATAAATAAAGTGAAGCAAGAAAGGAGCATCACACCAAAACTAGTGTTTATCCGAGGAGGACGAGACGATGCTACTGTCTTTGAGAACTATCTTATAGAAGAACAAGATGTGGACGGTAATGGTTTTGCTAGCGCCATGGGTTTCGTGTCTTTCCTCGATGATATTTCGCAGCGTGTCACTGAATACATGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

745

Amino Acids

82.58

Weight (kDa)

4.81

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 51 - 84 1.9e-07 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 121 - 373 2.1e-27 Sec23/Sec24 trunk domain
Sec23_BS PF08033 379 - 451 1.9e-06 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 499 - 611 3.7e-14 Sec23/Sec24 helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1166
Acc36I ACCTGC 1 cut(s) 692
AccBSI CCGCTC 1 cut(s) 440
AccII CGCG 3 cut(s) 25, 95, 1894
AciI CCGC 7 cut(s) 95, 129, 377, 438, 1056, 1839, 1892
AclWI GGATC 7 cut(s) 139, 343, 645, 787, 1205, 1445, 1503
AcoI YGGCCR 2 cut(s) 96, 1127
AcsI RAATTY 6 cut(s) 404, 785, 1172, 1280, 1381, 1479
AcuI CTGAAG 3 cut(s) 1052, 1214, 1406
AcyI GRCGYC 2 cut(s) 56, 1163
AfaI GTAC 5 cut(s) 748, 1013, 1220, 1349, 1362
AfiI CCNNNNNNNGG 8 cut(s) 128, 332, 557, 558, 559, 845, 1370, 1752
AflIII ACRYGT 1 cut(s) 1107
AhlI ACTAGT 1 cut(s) 2083
AjiI CACGTC 1 cut(s) 851
AjnI CCWGG 3 cut(s) 457, 1180, 1304
AjuI GAANNNNNNNTTGG 2 cut(s) 1095, 1127
AluBI AGCT 9 cut(s) 196, 415, 449, 570, 695, 752, 761, 809, 1827
AluI AGCT 9 cut(s) 196, 415, 449, 570, 695, 752, 761, 809, 1827
Alw21I GWGCWC 2 cut(s) 919, 1829
Alw26I GTCTC 3 cut(s) 653, 1420, 2100
AlwI GGATC 7 cut(s) 139, 343, 645, 787, 1205, 1445, 1503
Ama87I CYCGRG 2 cut(s) 1367, 1822
AoxI GGCC 2 cut(s) 96, 1127
ApeKI GCWGC 6 cut(s) 567, 647, 650, 1579, 1913, 2212
ApoI RAATTY 6 cut(s) 404, 785, 1172, 1280, 1381, 1479
ArsI GACNNNNNNTTYG 2 cut(s) 1265, 1297
Asp700I GAANNNNTTC 3 cut(s) 542, 1506, 1770
AspLEI GCGC 1 cut(s) 2177
AspS9I GGNCC 4 cut(s) 119, 518, 833, 1819
AsuHPI GGTGA 1 cut(s) 890
AsuII TTCGAA 1 cut(s) 1114
AsuNHI GCTAGC 2 cut(s) 433, 2171
AvaI CYCGRG 2 cut(s) 1367, 1822
AvaII GGWCC 4 cut(s) 119, 518, 833, 1819
BanII GRGCYC 2 cut(s) 466, 1829
BbsI GAAGAC 1 cut(s) 1966
Bbv12I GWGCWC 2 cut(s) 919, 1829
BbvI GCAGC 6 cut(s) 579, 634, 637, 1566, 1925, 2224
BccI CCATC 5 cut(s) 326, 551, 706, 721, 1547
BceAI ACGGC 2 cut(s) 1114, 1893
BcgI CGANNNNNNTGC 1 cut(s) 37
BciT130I CCWGG 3 cut(s) 459, 1182, 1306
BciVI GTATCC 2 cut(s) 856, 1870
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 3 cut(s) 653, 1420, 2100
BcuI ACTAGT 1 cut(s) 2083
BfaI CTAG 9 cut(s) 434, 578, 776, 810, 953, 1592, 1949, 2084, 2172
BfoI RGCGCY 1 cut(s) 2178
BfuAI ACCTGC 1 cut(s) 692
BfuI GTATCC 2 cut(s) 856, 1870
Bme1390I CCNGG 3 cut(s) 459, 1182, 1306
Bme18I GGWCC 4 cut(s) 119, 518, 833, 1819
BmeT110I CYCGRG 2 cut(s) 1367, 1822
BmgBI CACGTC 1 cut(s) 851
BmgT120I GGNCC 4 cut(s) 119, 518, 833, 1819
BmiI GGNNCC 2 cut(s) 618, 1821
BmrFI CCNGG 3 cut(s) 459, 1182, 1306
BmsI GCATC 8 cut(s) 244, 312, 895, 1034, 1609, 1942, 2079, 2101
BmtI GCTAGC 2 cut(s) 437, 2175
BpiI GAAGAC 1 cut(s) 1966
BpmI CTGGAG 2 cut(s) 717, 1335
Bpu10I CCTNAGC 1 cut(s) 1040
Bpu14I TTCGAA 1 cut(s) 1114
BpuEI CTTGAG 7 cut(s) 33, 338, 605, 677, 1171, 1724, 2004
Bsa29I ATCGAT 1 cut(s) 36
BsaBI GATNNNNATC 3 cut(s) 386, 909, 1676
BsaHI GRCGYC 2 cut(s) 56, 1163
BsaJI CCNNGG 5 cut(s) 68, 458, 1368, 2095, 2178
BsaWI WCCGGW 3 cut(s) 619, 1752, 1873
Bsc4I CCNNNNNNNGG 8 cut(s) 128, 332, 557, 558, 559, 845, 1370, 1752
Bse118I RCCGGY 1 cut(s) 1125
Bse1I ACTGG 4 cut(s) 527, 1300, 1325, 1887
Bse3DI GCAATG 6 cut(s) 428, 960, 1514, 1574, 1805, 1834
Bse8I GATNNNNATC 3 cut(s) 386, 909, 1676
BseBI CCWGG 3 cut(s) 459, 1182, 1306
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 5 cut(s) 68, 458, 1368, 2095, 2178
BseGI GGATG 5 cut(s) 337, 595, 717, 1025, 1774
BseJI GATNNNNATC 3 cut(s) 386, 909, 1676
BseLI CCNNNNNNNGG 8 cut(s) 128, 332, 557, 558, 559, 845, 1370, 1752
BseMI GCAATG 6 cut(s) 428, 960, 1514, 1574, 1805, 1834
BseMII CTCAG 3 cut(s) 390, 679, 1682
BseNI ACTGG 4 cut(s) 527, 1300, 1325, 1887
BseRI GAGGAG 1 cut(s) 2112
BseX3I CGGCCG 1 cut(s) 1127
BseXI GCAGC 6 cut(s) 579, 634, 637, 1566, 1925, 2224
BseYI CCCAGC 1 cut(s) 1849
BsgI GTGCAG 2 cut(s) 636, 1932
Bsh1236I CGCG 3 cut(s) 25, 95, 1894
Bsh1285I CGRYCG 2 cut(s) 36, 1130
BshFI GGCC 2 cut(s) 98, 1129
BshVI ATCGAT 1 cut(s) 36
BsiEI CGRYCG 2 cut(s) 36, 1130
BsiHKAI GWGCWC 2 cut(s) 919, 1829
BsiHKCI CYCGRG 2 cut(s) 1367, 1822
BsiSI CCGG 4 cut(s) 620, 1126, 1753, 1874
BslFI GGGAC 2 cut(s) 87, 1386
BslI CCNNNNNNNGG 8 cut(s) 128, 332, 557, 558, 559, 845, 1370, 1752
BsmAI GTCTC 3 cut(s) 653, 1420, 2100
BsmBI CGTCTC 2 cut(s) 1420, 2100
BsmFI GGGAC 2 cut(s) 87, 1386
BsmI GAATGC 3 cut(s) 1122, 1136, 1309
BsnI GGCC 2 cut(s) 98, 1129
BsoBI CYCGRG 2 cut(s) 1367, 1822
Bsp119I TTCGAA 1 cut(s) 1114
Bsp1286I GDGCHC 3 cut(s) 466, 919, 1829
Bsp19I CCATGG 1 cut(s) 2178
BspACI CCGC 7 cut(s) 95, 129, 377, 438, 1056, 1839, 1892
BspANI GGCC 2 cut(s) 98, 1129
BspCNI CTCAG 3 cut(s) 391, 678, 1681
BspDI ATCGAT 1 cut(s) 36
BspFNI CGCG 3 cut(s) 25, 95, 1894
BspHI TCATGA 2 cut(s) 1498, 1759
BspLI GGNNCC 2 cut(s) 618, 1821
BspMI ACCTGC 1 cut(s) 692
BspOI GCTAGC 2 cut(s) 437, 2175
BspPI GGATC 7 cut(s) 139, 343, 645, 787, 1205, 1445, 1503
BspT104I TTCGAA 1 cut(s) 1114
BsrBI CCGCTC 1 cut(s) 440
BsrDI GCAATG 6 cut(s) 428, 960, 1514, 1574, 1805, 1834
BsrFI RCCGGY 1 cut(s) 1125
BsrI ACTGG 4 cut(s) 527, 1300, 1325, 1887
BssAI RCCGGY 1 cut(s) 1125
BssECI CCNNGG 5 cut(s) 68, 458, 1368, 2095, 2178
BssNI GRCGYC 2 cut(s) 56, 1163
BssT1I CCWWGG 2 cut(s) 68, 2178
Bst2UI CCWGG 3 cut(s) 459, 1182, 1306
Bst6I CTCTTC 4 cut(s) 339, 397, 1101, 1836
BstACI GRCGYC 2 cut(s) 56, 1163
BstAPI GCANNNNNTGC 1 cut(s) 1265
BstBI TTCGAA 1 cut(s) 1114
BstC8I GCNNGC 6 cut(s) 127, 221, 357, 435, 1412, 2173
BstDEI CTNAG 5 cut(s) 399, 452, 665, 1040, 1668
BstDSI CCRYGG 1 cut(s) 2178
BstF5I GGATG 5 cut(s) 337, 595, 717, 1025, 1774
BstFNI CGCG 3 cut(s) 25, 95, 1894
BstH2I RGCGCY 1 cut(s) 2178
BstHHI GCGC 1 cut(s) 2177
BstMAI GTCTC 3 cut(s) 653, 1420, 2100
BstMCI CGRYCG 2 cut(s) 36, 1130
BstNI CCWGG 3 cut(s) 459, 1182, 1306
BstNSI RCATGY 1 cut(s) 1111
BstSCI CCNGG 3 cut(s) 457, 1180, 1304
BstUI CGCG 3 cut(s) 25, 95, 1894
BstV1I GCAGC 6 cut(s) 579, 634, 637, 1566, 1925, 2224
BstV2I GAAGAC 1 cut(s) 1966
BstX2I RGATCY 3 cut(s) 131, 779, 1210
BstYI RGATCY 3 cut(s) 131, 779, 1210
BstZI CGGCCG 1 cut(s) 1127
Bsu15I ATCGAT 1 cut(s) 36
BsuI GTATCC 2 cut(s) 856, 1870
BsuRI GGCC 2 cut(s) 98, 1129
BsuTUI ATCGAT 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 2178
BtrI CACGTC 1 cut(s) 851
BtsCI GGATG 5 cut(s) 337, 595, 717, 1025, 1774
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 9 cut(s) 12, 1061, 1080, 1293, 1332, 1495, 1816, 1880, 2220
BveI ACCTGC 1 cut(s) 692
Cac8I GCNNGC 6 cut(s) 127, 221, 357, 435, 1412, 2173
CciI TCATGA 2 cut(s) 1498, 1759
CfoI GCGC 1 cut(s) 2177
Cfr10I RCCGGY 1 cut(s) 1125
Cfr13I GGNCC 4 cut(s) 119, 518, 833, 1819
ClaI ATCGAT 1 cut(s) 36
CseI GACGC 4 cut(s) 45, 53, 361, 1883
Csp6I GTAC 5 cut(s) 747, 1012, 1219, 1348, 1361
CviAII CATG 8 cut(s) 595, 1108, 1447, 1499, 1583, 1760, 2179, 2230
CviQI GTAC 5 cut(s) 747, 1012, 1219, 1348, 1361
DdeI CTNAG 5 cut(s) 399, 452, 665, 1040, 1668
EaeI YGGCCR 2 cut(s) 96, 1127
EagI CGGCCG 1 cut(s) 1127
Eam1104I CTCTTC 4 cut(s) 339, 397, 1101, 1836
EarI CTCTTC 4 cut(s) 339, 397, 1101, 1836
Ecl136II GAGCTC 1 cut(s) 1827
EclXI CGGCCG 1 cut(s) 1127
Eco130I CCWWGG 2 cut(s) 68, 2178
Eco24I GRGCYC 2 cut(s) 466, 1829
Eco32I GATATC 2 cut(s) 388, 1678
Eco47I GGWCC 4 cut(s) 119, 518, 833, 1819
Eco52I CGGCCG 1 cut(s) 1127
Eco53kI GAGCTC 1 cut(s) 1827
Eco57I CTGAAG 3 cut(s) 1052, 1214, 1406
Eco88I CYCGRG 2 cut(s) 1367, 1822
EcoICRI GAGCTC 1 cut(s) 1827
EcoO109I RGGNCCY 1 cut(s) 518
EcoRI GAATTC 1 cut(s) 1172
EcoRII CCWGG 3 cut(s) 457, 1180, 1304
EcoRV GATATC 2 cut(s) 388, 1678
EcoT14I CCWWGG 2 cut(s) 68, 2178
EcoT22I ATGCAT 3 cut(s) 1138, 1270, 1957
EcoT38I GRGCYC 2 cut(s) 466, 1829
ErhI CCWWGG 2 cut(s) 68, 2178
Esp3I CGTCTC 2 cut(s) 1420, 2100
FaeI CATG 8 cut(s) 598, 1111, 1450, 1502, 1586, 1763, 2182, 2233
FaqI GGGAC 2 cut(s) 87, 1386
FatI CATG 8 cut(s) 594, 1107, 1446, 1498, 1582, 1759, 2178, 2229
FauNDI CATATG 3 cut(s) 1255, 1264, 1270
FbaI TGATCA 1 cut(s) 199
FokI GGATG 5 cut(s) 344, 602, 724, 1012, 1761
FriOI GRGCYC 2 cut(s) 466, 1829
FspBI CTAG 9 cut(s) 434, 578, 776, 810, 953, 1592, 1949, 2084, 2172
GlaI GCGC 1 cut(s) 2176
GsaI CCCAGC 1 cut(s) 1853
GsuI CTGGAG 2 cut(s) 717, 1335
HaeII RGCGCY 1 cut(s) 2178
HaeIII GGCC 2 cut(s) 98, 1129
HapII CCGG 4 cut(s) 620, 1126, 1753, 1874
HgaI GACGC 4 cut(s) 45, 53, 361, 1883
HhaI GCGC 1 cut(s) 2177
Hin1I GRCGYC 2 cut(s) 56, 1163
Hin1II CATG 8 cut(s) 598, 1111, 1450, 1502, 1586, 1763, 2182, 2233
Hin6I GCGC 1 cut(s) 2175
HinP1I GCGC 1 cut(s) 2175
HincII GTYRAC 1 cut(s) 1167
HindII GTYRAC 1 cut(s) 1167
HinfI GANTC 6 cut(s) 38, 114, 1088, 1250, 1557, 1788
HpaII CCGG 4 cut(s) 620, 1126, 1753, 1874
HphI GGTGA 1 cut(s) 890
Hpy166II GTNNAC 8 cut(s) 854, 881, 1014, 1061, 1167, 1350, 1525, 2155
Hpy8I GTNNAC 8 cut(s) 854, 881, 1014, 1061, 1167, 1350, 1525, 2155
HpyAV CCTTC 6 cut(s) 258, 316, 1238, 1559, 1757, 1760
HpyCH4IV ACGT 4 cut(s) 482, 850, 1163, 1682
HpyF3I CTNAG 5 cut(s) 399, 452, 665, 1040, 1668
HpySE526I ACGT 4 cut(s) 482, 850, 1163, 1682
Hsp92I GRCGYC 2 cut(s) 56, 1163
Hsp92II CATG 8 cut(s) 598, 1111, 1450, 1502, 1586, 1763, 2182, 2233
HspAI GCGC 1 cut(s) 2175
Ksp22I TGATCA 1 cut(s) 199
LmnI GCTCC 4 cut(s) 412, 622, 698, 2067
Lsp1109I GCAGC 6 cut(s) 579, 634, 637, 1566, 1925, 2224
LweI GCATC 8 cut(s) 244, 312, 895, 1034, 1609, 1942, 2079, 2101
MaeI CTAG 9 cut(s) 434, 578, 776, 810, 953, 1592, 1949, 2084, 2172
MaeII ACGT 4 cut(s) 482, 850, 1163, 1682
MaeIII GTNAC 8 cut(s) 82, 266, 478, 488, 603, 1000, 1196, 2218
MbiI CCGCTC 1 cut(s) 440
MfeI CAATTG 2 cut(s) 857, 1141
MflI RGATCY 3 cut(s) 131, 779, 1210
MhlI GDGCHC 3 cut(s) 466, 919, 1829
MlyI GAGTC 1 cut(s) 1566
MmeI TCCRAC 1 cut(s) 1797
Mph1103I ATGCAT 3 cut(s) 1138, 1270, 1957
MroXI GAANNNNTTC 3 cut(s) 542, 1506, 1770
MseI TTAA 3 cut(s) 1122, 1433, 1626
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 4 cut(s) 620, 1126, 1753, 1874
MspR9I CCNGG 3 cut(s) 459, 1182, 1306
MunI CAATTG 2 cut(s) 857, 1141
Mva1269I GAATGC 3 cut(s) 1122, 1136, 1309
MvaI CCWGG 3 cut(s) 459, 1182, 1306
MvnI CGCG 3 cut(s) 25, 95, 1894
NcoI CCATGG 1 cut(s) 2178
NdeI CATATG 3 cut(s) 1255, 1264, 1270
NheI GCTAGC 2 cut(s) 433, 2171
NlaIII CATG 8 cut(s) 598, 1111, 1450, 1502, 1586, 1763, 2182, 2233
NlaIV GGNNCC 2 cut(s) 618, 1821
NmuCI GTSAC 3 cut(s) 488, 1196, 2218
NsiI ATGCAT 3 cut(s) 1138, 1270, 1957
NspI RCATGY 1 cut(s) 1111
NspV TTCGAA 1 cut(s) 1114
PagI TCATGA 2 cut(s) 1498, 1759
PciI ACATGT 1 cut(s) 1107
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 3 cut(s) 1122, 1136, 1309
PdmI GAANNNNTTC 3 cut(s) 542, 1506, 1770
PfeI GAWTC 5 cut(s) 38, 114, 1088, 1250, 1788
Ple19I CGATCG 1 cut(s) 36
PleI GAGTC 1 cut(s) 1565
PpsI GAGTC 1 cut(s) 1565
PpuMI RGGWCCY 1 cut(s) 518
PscI ACATGT 1 cut(s) 1107
Psp124BI GAGCTC 1 cut(s) 1829
Psp5II RGGWCCY 1 cut(s) 518
Psp6I CCWGG 3 cut(s) 457, 1180, 1304
PspFI CCCAGC 1 cut(s) 1849
PspGI CCWGG 3 cut(s) 457, 1180, 1304
PspN4I GGNNCC 2 cut(s) 618, 1821
PspPI GGNCC 4 cut(s) 119, 518, 833, 1819
PspPPI RGGWCCY 1 cut(s) 518
PsuI RGATCY 3 cut(s) 131, 779, 1210
PvuI CGATCG 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 5 cut(s) 748, 1013, 1220, 1349, 1362
RsaNI GTAC 5 cut(s) 747, 1012, 1219, 1348, 1361
SacI GAGCTC 1 cut(s) 1829
SaqAI TTAA 3 cut(s) 1122, 1433, 1626
Sau96I GGNCC 4 cut(s) 119, 518, 833, 1819
SchI GAGTC 1 cut(s) 1566
ScrFI CCNGG 3 cut(s) 459, 1182, 1306
SduI GDGCHC 3 cut(s) 466, 919, 1829
SfaNI GCATC 8 cut(s) 244, 312, 895, 1034, 1609, 1942, 2079, 2101
SfuI TTCGAA 1 cut(s) 1114
SinI GGWCC 4 cut(s) 119, 518, 833, 1819
SmlI CTYRAG 7 cut(s) 48, 317, 584, 656, 1150, 1703, 2019
SmoI CTYRAG 7 cut(s) 48, 317, 584, 656, 1150, 1703, 2019
SpeI ACTAGT 1 cut(s) 2083
SsiI CCGC 7 cut(s) 95, 129, 377, 438, 1056, 1839, 1892
SspMI CTAG 9 cut(s) 434, 578, 776, 810, 953, 1592, 1949, 2084, 2172
SstI GAGCTC 1 cut(s) 1829
StyD4I CCNGG 3 cut(s) 457, 1180, 1304
StyI CCWWGG 2 cut(s) 68, 2178
TaiI ACGT 4 cut(s) 485, 853, 1166, 1685
TaqI TCGA 3 cut(s) 36, 1114, 2199
TatI WGTACW 2 cut(s) 1011, 1347
TauI GCSGC 4 cut(s) 98, 380, 440, 1842
TfiI GAWTC 5 cut(s) 38, 114, 1088, 1250, 1788
Tru1I TTAA 3 cut(s) 1122, 1433, 1626
Tru9I TTAA 3 cut(s) 1122, 1433, 1626
TscAI CASTG 9 cut(s) 12, 1068, 1087, 1300, 1332, 1495, 1816, 1887, 2227
TseFI GTSAC 3 cut(s) 488, 1196, 2218
TseI GCWGC 6 cut(s) 567, 647, 650, 1579, 1913, 2212
Tsp45I GTSAC 3 cut(s) 488, 1196, 2218
TspDTI ATGAA 9 cut(s) 298, 535, 1242, 1491, 1515, 1748, 1763, 1776, 1823
TspGWI ACGGA 3 cut(s) 126, 1662, 1727
TspRI CASTG 9 cut(s) 12, 1068, 1087, 1300, 1332, 1495, 1816, 1887, 2227
VpaK11BI GGWCC 4 cut(s) 119, 518, 833, 1819
XapI RAATTY 6 cut(s) 404, 785, 1172, 1280, 1381, 1479
XceI RCATGY 1 cut(s) 1111
XcmI CCANNNNNNNNNTGG 1 cut(s) 678
XmnI GAANNNNTTC 3 cut(s) 542, 1506, 1770
XspI CTAG 9 cut(s) 434, 578, 776, 810, 953, 1592, 1949, 2084, 2172
ZraI GACGTC 1 cut(s) 1164
Zsp2I ATGCAT 3 cut(s) 1138, 1270, 1957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.