Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0011378

Physical Location & Seq

Genomic Coordinates Reverse (-)

15923942 .. 15931622

Gene Structure 7681 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1145900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2241 bp

ATGGCGGTCCGAGCCTCCGTGTCGCGGTTTCCGATCGACGCCGGGGCCCACGAGAGCTCAGGCATCCCATGGGGCGTTTCGGTGACGCCGTTCGCGACGAAGGACGAGAACGGCCAGTCGCCGGCGTACGGATCGGACGGCCAGCTGCTGCCGAGGTGCGAGAACTGCTGGGCGTATTTCAACACGTTCTGCGAGCTGGATCAGTGGTCGTGGAACTGTGCCCTGTGCGGAAACCCCCAGGGGCTTTCGTCTCAGGCCATTGCTCGATACTCGCACCCTCAGTCCTGCCCCGAAATGATGTCCTCGTTCGTAGATCTGGAGCTTCCTGTAGAAGAGTCGGATGAAGTAATGCAACCTCGCCCAGTTTACGTTGCAGTCATTGATTTGTCCTCTTCAGAAGAATTTTTGGAACTTATTAAAAGTGCATTGCTGGCCTCCTTGGAAGCTCTTGGGCCCGGTTCTCTGTTTGGTCTTGCTACTTTTAGCCACAAATTAGGGTTGTATGATGTTCAAGGACCTATACCAGTTGTAAAGAATGTTTTCATTCCACCGGAAACAGACGGTACCCTACCAATTGAGCTTGAAGATGTCATGCCATTGTTACAGTTTTTGGCTCCAGTGGAAACTTGTAAGGACCGTATTGGATCTGCACTTGAAACGCTCAGACCAACAACTTCATGGGAGAGAACTACAGCAGCTGGTCAAGGAATTGATGGTGTTTTGATGGGCGGGCGAGGGTTTGGGGTGGCAATGGAAGCACTTTTGAACTACCTTGGATCTGAGTATGGAAGTACATTTGCATTAGGTAGAGTCTTTGCCTTTTTGTCTGGTCCTCCTGATTATGGAGCTGGGCAACTAGATACAAGACGTTATGGTGAACAATATGCTAGTAAGGGAGAGGATGCGGACCGTGCATTGCTTCCTGAGCAGACACCATTCTACAGAGATCTGGCTGCTGTCGCTGTTCAAGCAGGTGTTTGCGTGGACATTTTTGCTGTTACAAATGAGTATACAGATTTGGCGTCTTTGAAGTTTCTAAGTGTAGACAGTGGAGGCTCATTGTATTTATATTCGAACACTGATGACTCAACTCTTCCTCAGGACATGTACCGAATGCTAAGTCGGCCATATGCTTTTGGTTGTGTACTGCGATTGAGGACCTCATCTGAATTCAAAACTGGCCACTCTTATGGTCATTTCTTTCCGGATCCACAGTATGAGAATGTTCAACACATTATCTGTTGTGATTCTTATGCGACATATGCTTATGACTTTGAGTTTGAAGACACCAAGGGATTTGCAAAACATGGATCAGAGCCTCCTATTATACAGATTGCATTTCAATACACTGTTGTACTGCCTCCTAAGGAGCTCTCCGATTCAACATCCGTGTCCTCAAGTAGAGGCAAGTATTCCCTTAAACGACGACTAAGGATCCGAACATTACAATGTGGAGTTGCCCAAAATATGAATGAACTCTACGACAGTGTTGATCCTGAAGTAATCCTGTCACTGCTTGTTCACAAGGTTATACTAGCCTCTTTGGAGCAAGGAGTTCGGGAAGGCAGAATATTGCTTCATGATTGGCTTGTGATCCTAACAGCTCAATACAATGAAGCGTATAAACTAATTCAATACAAGAATGGGAGTGCAGGAACTGCTCAGATTGATGTTGCATTCACACAATGTCCAGAACTGCAGCCTTTGCCCCGCTTGGTTTTTGCATTGCTTCGAAATCCTCTGCTCCGTTTTCATGAAGAAGGTGTTCATCCGGACTACCGAATCTACCTGCAGTGCCTTTTCAGTGCACTGGAACCAAGTTCACTTCATCGTGCCGTGTATCCAGTGCTGACCTCCTATTCTACTCCAGACAAACAAGCATACCCACGCCATTCACCGAGTCGTGCAGCGCTGATTACCAGTGGTAGTCCTATATTTTTCCTTGATGCATTTACGTCTCTGATTGTGTTCTATTCCTCAACGGCAGACCCTTCACTTCCTTTTCCTCCACCTCATGATTGTTTGTTGCGGACTGTGATCAACAATTTGAAGAAAGAGAGATGCATCACCCCCAAACTCATATTTATCAGAGGAGGGCAGGACGATGCTACACCTTTCGAGAATTATCTTATTGAGGAACAGGATGTTGACGGAAGCGGTATAACGAGCGTTATGGGTTTTGTTTCCTTTCTGGAGGATGTTACGCAGAGTGTGCTAGAATACATGAAGTCTGGTATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

747

Amino Acids

82.47

Weight (kDa)

4.85

Isoelectric Point (pI)

43.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec23_trunk	PF04811	120 - 372	1.3e-27	Sec23/Sec24 trunk domain
Sec23_helical	PF04815	498 - 609	1.2e-14	Sec23/Sec24 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011378)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27460
fragaria_vesca	FvH4_3g24980
malus_domestica	MD03G1145900.v1.1 MD11G1164800.v1.1
prunus_persica	Prupe.6G130700_v2.0.a1 Prupe.6G130700_v2.0.a1
pyrus_communis	pycom03g10000 pycom439g00040
rosa_chinensis	RchiOBHm_Chr5g0040981 RchiOBHm_Chr5g0044841 RchiOBHm_Chr5g0045601
rosa_laevigata	RLG00000034301
rosa_multiflora	Rmu_sc0004567.1_g000018
rosa_roxburghii	Rroxscaffold_1G00034820 Rroxscaffold_1G00036200
rosa_rugosa	Rorug05G0219300
rosa_samantha	Rh5AG301100 Rh5CG335200 Rh5DG318500
rosa_wichuraiana	Rw5G027910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	962
Acc36I	ACCTGC	2 cut(s)	962, 1797
Acc65I	GGTACC	1 cut(s)	563
AccB1I	GGYRCC	1 cut(s)	563
AccI	GTMKAC	2 cut(s)	1010, 1044
AccII	CGCG	2 cut(s)	25, 95
AccIII	TCCGGA	2 cut(s)	1204, 1771
AciI	CCGC	8 cut(s)	5, 25, 228, 729, 905, 1711, 2029, 2157
AcoI	YGGCCR	4 cut(s)	112, 139, 1124, 1180
AcsI	RAATTY	2 cut(s)	401, 1169
AcuI	CTGAAG	2 cut(s)	378, 1518
AcyI	GRCGYC	3 cut(s)	39, 86, 1022
AfaI	GTAC	6 cut(s)	128, 565, 793, 1109, 1146, 1356
AfeI	AGCGCT	1 cut(s)	1911
AfiI	CCNNNNNNNGG	4 cut(s)	24, 121, 128, 842
AflIII	ACRYGT	2 cut(s)	183, 1104
AjnI	CCWGG	1 cut(s)	237
Alw21I	GWGCWC	3 cut(s)	59, 1374, 1810
Alw26I	GTCTC	2 cut(s)	255, 1962
Alw44I	GTGCAC	1 cut(s)	1806
AlwNI	CAGNNNCTG	3 cut(s)	148, 698, 1658
Aor13HI	TCCGGA	2 cut(s)	1204, 1771
Aor51HI	AGCGCT	1 cut(s)	1911
AoxI	GGCC	8 cut(s)	45, 112, 139, 255, 432, 452, 1124, 1180
ApaI	GGGCCC	2 cut(s)	49, 456
ApaLI	GTGCAC	1 cut(s)	1806
ApeKI	GCWGC	6 cut(s)	145, 148, 695, 953, 1699, 1907
ApoI	RAATTY	2 cut(s)	401, 1169
ArsI	GACNNNNNNTTYG	4 cut(s)	1105, 1137, 1262, 1294
Asp700I	GAANNNNTTC	2 cut(s)	539, 1764
Asp718I	GGTACC	1 cut(s)	563
AspLEI	GCGC	1 cut(s)	1912
AsuC2I	CCSGG	2 cut(s)	43, 456
AsuHPI	GGTGA	4 cut(s)	94, 887, 1887, 2059
AsuII	TTCGAA	2 cut(s)	1073, 1732
AvaII	GGWCC	6 cut(s)	7, 515, 634, 830, 907, 1158
AxyI	CCTNAGG	2 cut(s)	1098, 1365
BaeGI	GKGCMC	4 cut(s)	49, 223, 456, 1810
BalI	TGGCCA	1 cut(s)	1182
BamHI	GGATCC	2 cut(s)	1207, 1434
BanI	GGYRCC	1 cut(s)	563
BanII	GRGCYC	4 cut(s)	49, 59, 456, 1374
BauI	CACGAG	1 cut(s)	50
BbsI	GAAGAC	1 cut(s)	1290
Bbv12I	GWGCWC	3 cut(s)	59, 1374, 1810
BbvI	GCAGC	6 cut(s)	132, 135, 707, 940, 1711, 1919
BccI	CCATC	2 cut(s)	707, 718
BceAI	ACGGC	5 cut(s)	73, 127, 154, 1820, 1998
BcgI	CGANNNNNNTGC	2 cut(s)	1538, 1572
BciT130I	CCWGG	1 cut(s)	239
BciVI	GTATCC	1 cut(s)	1851
BclI	TGATCA	1 cut(s)	2037
BcnI	CCSGG	2 cut(s)	43, 456
BcoDI	GTCTC	2 cut(s)	255, 1962
BfaI	CTAG	4 cut(s)	857, 888, 1535, 2216
BfmI	CTRYAG	5 cut(s)	327, 690, 940, 1697, 1790
BfoI	RGCGCY	1 cut(s)	1913
BfuAI	ACCTGC	2 cut(s)	962, 1797
BfuI	GTATCC	1 cut(s)	1851
BglII	AGATCT	2 cut(s)	313, 946
BisI	GCNGC	6 cut(s)	146, 149, 696, 954, 1700, 1908
BlsI	GCNGC	6 cut(s)	147, 150, 697, 955, 1701, 1909
Bme1390I	CCNGG	3 cut(s)	43, 239, 456
Bme18I	GGWCC	6 cut(s)	7, 515, 634, 830, 907, 1158
BmiI	GGNNCC	8 cut(s)	46, 47, 454, 565, 615, 1209, 1436, 1815
BmrFI	CCNGG	3 cut(s)	43, 239, 456
BmrI	ACTGGG	1 cut(s)	356
BmsI	GCATC	6 cut(s)	72, 892, 1936, 2051, 2073, 2095
BmuI	ACTGGG	1 cut(s)	356
BpiI	GAAGAC	1 cut(s)	1290
BpmI	CTGGAG	4 cut(s)	338, 600, 1851, 2213
Bpu10I	CCTNAGC	2 cut(s)	58, 924
Bpu14I	TTCGAA	2 cut(s)	1073, 1732
BpuEI	CTTGAG	1 cut(s)	1381
BpuMI	CCSGG	2 cut(s)	43, 456
BsaHI	GRCGYC	3 cut(s)	39, 86, 1022
BsaJI	CCNNGG	8 cut(s)	42, 68, 152, 237, 238, 438, 772, 1290
BsaWI	WCCGGW	3 cut(s)	550, 1204, 1771
Bsc4I	CCNNNNNNNGG	4 cut(s)	24, 121, 128, 842
Bse118I	RCCGGY	1 cut(s)	121
Bse1I	ACTGG	8 cut(s)	115, 362, 524, 617, 1183, 1815, 1844, 1920
Bse21I	CCTNAGG	2 cut(s)	1098, 1365
Bse3DI	GCAATG	5 cut(s)	258, 425, 756, 914, 1724
BseAI	TCCGGA	2 cut(s)	1204, 1771
BseBI	CCWGG	1 cut(s)	239
BseDI	CCNNGG	8 cut(s)	42, 68, 152, 237, 238, 438, 772, 1290
BseGI	GGATG	7 cut(s)	63, 346, 907, 1385, 1768, 2149, 2203
BseLI	CCNNNNNNNGG	4 cut(s)	24, 121, 128, 842
BseMI	GCAATG	5 cut(s)	258, 425, 756, 914, 1724
BseMII	CTCAG	8 cut(s)	72, 266, 293, 676, 771, 915, 1112, 1676
BseNI	ACTGG	8 cut(s)	115, 362, 524, 617, 1183, 1815, 1844, 1920
BseRI	GAGGAG	1 cut(s)	2106
BseSI	GKGCMC	4 cut(s)	49, 223, 456, 1810
BseXI	GCAGC	6 cut(s)	132, 135, 707, 940, 1711, 1919
BseYI	CCCAGC	2 cut(s)	168, 848
BsgI	GTGCAG	3 cut(s)	633, 1671, 1926
Bsh1236I	CGCG	2 cut(s)	25, 95
Bsh1285I	CGRYCG	1 cut(s)	36
BshFI	GGCC	8 cut(s)	47, 114, 141, 257, 434, 454, 1126, 1182
BshNI	GGYRCC	1 cut(s)	563
BsiEI	CGRYCG	1 cut(s)	36
BsiHKAI	GWGCWC	3 cut(s)	59, 1374, 1810
BsiSI	CCGG	6 cut(s)	42, 122, 456, 551, 1205, 1772
BsiWI	CGTACG	1 cut(s)	126
BslI	CCNNNNNNNGG	4 cut(s)	24, 121, 128, 842
BsmAI	GTCTC	2 cut(s)	255, 1962
BsmBI	CGTCTC	2 cut(s)	255, 1962
BsmI	GAATGC	2 cut(s)	1119, 1676
BsnI	GGCC	8 cut(s)	47, 114, 141, 257, 434, 454, 1126, 1182
Bsp119I	TTCGAA	2 cut(s)	1073, 1732
Bsp120I	GGGCCC	2 cut(s)	45, 452
Bsp1286I	GDGCHC	6 cut(s)	49, 59, 223, 456, 1374, 1810
Bsp13I	TCCGGA	2 cut(s)	1204, 1771
Bsp19I	CCATGG	1 cut(s)	68
Bsp68I	TCGCGA	1 cut(s)	95
BspACI	CCGC	8 cut(s)	5, 25, 228, 729, 905, 1711, 2029, 2157
BspANI	GGCC	8 cut(s)	47, 114, 141, 257, 434, 454, 1126, 1182
BspCNI	CTCAG	8 cut(s)	71, 265, 292, 675, 772, 916, 1111, 1675
BspEI	TCCGGA	2 cut(s)	1204, 1771
BspFNI	CGCG	2 cut(s)	25, 95
BspHI	TCATGA	3 cut(s)	1579, 1753, 2014
BspLI	GGNNCC	8 cut(s)	46, 47, 454, 565, 615, 1209, 1436, 1815
BspMAI	CTGCAG	2 cut(s)	1701, 1794
BspMI	ACCTGC	2 cut(s)	962, 1797
BspT104I	TTCGAA	2 cut(s)	1073, 1732
BspT107I	GGYRCC	1 cut(s)	563
BsrDI	GCAATG	5 cut(s)	258, 425, 756, 914, 1724
BsrFI	RCCGGY	1 cut(s)	121
BsrI	ACTGG	8 cut(s)	115, 362, 524, 617, 1183, 1815, 1844, 1920
BssAI	RCCGGY	1 cut(s)	121
BssECI	CCNNGG	8 cut(s)	42, 68, 152, 237, 238, 438, 772, 1290
BssNAI	GTATAC	1 cut(s)	1011
BssNI	GRCGYC	3 cut(s)	39, 86, 1022
BssSI	CACGAG	1 cut(s)	50
BssT1I	CCWWGG	4 cut(s)	68, 438, 772, 1290
Bst1107I	GTATAC	1 cut(s)	1011
Bst2BI	CACGAG	1 cut(s)	50
Bst2UI	CCWGG	1 cut(s)	239
Bst6I	CTCTTC	3 cut(s)	327, 397, 1098
BstACI	GRCGYC	3 cut(s)	39, 86, 1022
BstAPI	GCANNNNNTGC	3 cut(s)	1658, 1705, 2212
BstBI	TTCGAA	2 cut(s)	1073, 1732
BstC8I	GCNNGC	5 cut(s)	123, 143, 194, 432, 731
BstDSI	CCRYGG	1 cut(s)	68
BstF5I	GGATG	7 cut(s)	63, 346, 907, 1385, 1768, 2149, 2203
BstFNI	CGCG	2 cut(s)	25, 95
BstH2I	RGCGCY	1 cut(s)	1913
BstHHI	GCGC	1 cut(s)	1912
BstMAI	GTCTC	2 cut(s)	255, 1962
BstMCI	CGRYCG	1 cut(s)	36
BstNI	CCWGG	1 cut(s)	239
BstNSI	RCATGY	1 cut(s)	1108
BstSCI	CCNGG	3 cut(s)	41, 237, 454
BstSFI	CTRYAG	5 cut(s)	327, 690, 940, 1697, 1790
BstSLI	GKGCMC	4 cut(s)	49, 223, 456, 1810
BstUI	CGCG	2 cut(s)	25, 95
BstV1I	GCAGC	6 cut(s)	132, 135, 707, 940, 1711, 1919
BstV2I	GAAGAC	1 cut(s)	1290
BstX2I	RGATCY	6 cut(s)	313, 644, 776, 946, 1207, 1434
BstXI	CCANNNNNNTGG	1 cut(s)	1190
BstYI	RGATCY	6 cut(s)	313, 644, 776, 946, 1207, 1434
BstZ17I	GTATAC	1 cut(s)	1011
Bsu36I	CCTNAGG	2 cut(s)	1098, 1365
BsuI	GTATCC	1 cut(s)	1851
BsuRI	GGCC	8 cut(s)	47, 114, 141, 257, 434, 454, 1126, 1182
BtgI	CCRYGG	1 cut(s)	68
BtsCI	GGATG	7 cut(s)	63, 346, 907, 1385, 1768, 2149, 2203
BtsI	GCAGTG	2 cut(s)	1511, 1799
BtuMI	TCGCGA	1 cut(s)	95
BveI	ACCTGC	2 cut(s)	962, 1797
Cac8I	GCNNGC	5 cut(s)	123, 143, 194, 432, 731
CaiI	CAGNNNCTG	3 cut(s)	148, 698, 1658
CciI	TCATGA	3 cut(s)	1579, 1753, 2014
CfoI	GCGC	1 cut(s)	1912
Cfr10I	RCCGGY	1 cut(s)	121
CpoI	CGGWCCG	2 cut(s)	7, 907
CseI	GACGC	3 cut(s)	47, 94, 1011
Csp6I	GTAC	6 cut(s)	127, 564, 792, 1108, 1145, 1355
CspI	CGGWCCG	2 cut(s)	7, 907
CviAII	CATG	9 cut(s)	69, 592, 678, 1105, 1307, 1580, 1754, 2015, 2224
CviQI	GTAC	6 cut(s)	127, 564, 792, 1108, 1145, 1355
EaeI	YGGCCR	4 cut(s)	112, 139, 1124, 1180
Eam1104I	CTCTTC	3 cut(s)	327, 397, 1098
EarI	CTCTTC	3 cut(s)	327, 397, 1098
Ecl136II	GAGCTC	2 cut(s)	57, 1372
Eco130I	CCWWGG	4 cut(s)	68, 438, 772, 1290
Eco24I	GRGCYC	4 cut(s)	49, 59, 456, 1374
Eco47I	GGWCC	6 cut(s)	7, 515, 634, 830, 907, 1158
Eco47III	AGCGCT	1 cut(s)	1911
Eco53kI	GAGCTC	2 cut(s)	57, 1372
Eco57I	CTGAAG	2 cut(s)	378, 1518
Eco81I	CCTNAGG	2 cut(s)	1098, 1365
EcoICRI	GAGCTC	2 cut(s)	57, 1372
EcoO109I	RGGNCCY	3 cut(s)	45, 515, 1158
EcoRI	GAATTC	1 cut(s)	1169
EcoRII	CCWGG	1 cut(s)	237
EcoT14I	CCWWGG	4 cut(s)	68, 438, 772, 1290
EcoT22I	ATGCAT	2 cut(s)	1951, 2066
EcoT38I	GRGCYC	4 cut(s)	49, 59, 456, 1374
ErhI	CCWWGG	4 cut(s)	68, 438, 772, 1290
Esp3I	CGTCTC	2 cut(s)	255, 1962
FaeI	CATG	9 cut(s)	72, 595, 681, 1108, 1310, 1583, 1757, 2018, 2227
FatI	CATG	9 cut(s)	68, 591, 677, 1104, 1306, 1579, 1753, 2014, 2223
FauI	CCCGC	2 cut(s)	722, 1718
FauNDI	CATATG	2 cut(s)	1129, 1261
FbaI	TGATCA	1 cut(s)	2037
FblI	GTMKAC	2 cut(s)	1010, 1044
Fnu4HI	GCNGC	6 cut(s)	146, 149, 696, 954, 1700, 1908
FokI	GGATG	7 cut(s)	50, 353, 914, 1372, 1755, 2156, 2210
FriOI	GRGCYC	4 cut(s)	49, 59, 456, 1374
Fsp4HI	GCNGC	6 cut(s)	146, 149, 696, 954, 1700, 1908
FspBI	CTAG	4 cut(s)	857, 888, 1535, 2216
GlaI	GCGC	1 cut(s)	1911
GluI	GCNGC	6 cut(s)	146, 149, 696, 954, 1700, 1908
GsaI	CCCAGC	2 cut(s)	172, 852
GsuI	CTGGAG	4 cut(s)	338, 600, 1851, 2213
HaeII	RGCGCY	1 cut(s)	1913
HaeIII	GGCC	8 cut(s)	47, 114, 141, 257, 434, 454, 1126, 1182
HapII	CCGG	6 cut(s)	42, 122, 456, 551, 1205, 1772
HgaI	GACGC	3 cut(s)	47, 94, 1011
HhaI	GCGC	1 cut(s)	1912
Hin1I	GRCGYC	3 cut(s)	39, 86, 1022
Hin1II	CATG	9 cut(s)	72, 595, 681, 1108, 1310, 1583, 1757, 2018, 2227
Hin6I	GCGC	1 cut(s)	1910
HinP1I	GCGC	1 cut(s)	1910
HincII	GTYRAC	1 cut(s)	2149
HindII	GTYRAC	1 cut(s)	2149
HinfI	GANTC	7 cut(s)	335, 810, 1085, 1247, 1379, 1782, 1900
HpaII	CCGG	6 cut(s)	42, 122, 456, 551, 1205, 1772
HphI	GGTGA	4 cut(s)	94, 887, 1887, 2059
Hpy99I	CGWCG	3 cut(s)	41, 100, 1428
HpyAV	CCTTC	4 cut(s)	94, 1556, 1754, 2001
HpyCH4IV	ACGT	4 cut(s)	185, 369, 868, 1955
HpySE526I	ACGT	4 cut(s)	185, 369, 868, 1955
Hsp92I	GRCGYC	3 cut(s)	39, 86, 1022
Hsp92II	CATG	9 cut(s)	72, 595, 681, 1108, 1310, 1583, 1757, 2018, 2227
HspAI	GCGC	1 cut(s)	1910
Kpn2I	TCCGGA	2 cut(s)	1204, 1771
KpnI	GGTACC	1 cut(s)	567
KroI	GCCGGC	1 cut(s)	121
KroNI	GCCGGC	1 cut(s)	123
Ksp22I	TGATCA	1 cut(s)	2037
LmnI	GCTCC	6 cut(s)	319, 619, 845, 1369, 1546, 1749
Lsp1109I	GCAGC	6 cut(s)	132, 135, 707, 940, 1711, 1919
LweI	GCATC	6 cut(s)	72, 892, 1936, 2051, 2073, 2095
MaeI	CTAG	4 cut(s)	857, 888, 1535, 2216
MaeII	ACGT	4 cut(s)	185, 369, 868, 1955
MaeIII	GTNAC	5 cut(s)	82, 600, 997, 1509, 2200
MboII	GAAGA	8 cut(s)	344, 384, 410, 596, 1085, 1295, 1769, 2062
MfeI	CAATTG	1 cut(s)	573
MflI	RGATCY	6 cut(s)	313, 644, 776, 946, 1207, 1434
MhlI	GDGCHC	6 cut(s)	49, 59, 223, 456, 1374, 1810
MlsI	TGGCCA	1 cut(s)	1182
MluCI	AATT	8 cut(s)	401, 491, 573, 708, 1169, 1629, 2044, 2122
MluNI	TGGCCA	1 cut(s)	1182
MlyI	GAGTC	4 cut(s)	344, 819, 1079, 1909
MmeI	TCCRAC	1 cut(s)	318
Mox20I	TGGCCA	1 cut(s)	1182
Mph1103I	ATGCAT	2 cut(s)	1951, 2066
MreI	CGCCGGCG	1 cut(s)	121
MroI	TCCGGA	2 cut(s)	1204, 1771
MroNI	GCCGGC	1 cut(s)	121
MroXI	GAANNNNTTC	2 cut(s)	539, 1764
MscI	TGGCCA	1 cut(s)	1182
MseI	TTAA	2 cut(s)	417, 1419
MslI	CAYNNNNRTG	2 cut(s)	1188, 1447
Msp20I	TGGCCA	1 cut(s)	1182
MspA1I	CMGCKG	2 cut(s)	145, 698
MspI	CCGG	6 cut(s)	42, 122, 456, 551, 1205, 1772
MspR9I	CCNGG	3 cut(s)	43, 239, 456
MunI	CAATTG	1 cut(s)	573
Mva1269I	GAATGC	2 cut(s)	1119, 1676
MvaI	CCWGG	1 cut(s)	239
MvnI	CGCG	2 cut(s)	25, 95
NaeI	GCCGGC	1 cut(s)	123
NciI	CCSGG	2 cut(s)	43, 456
NcoI	CCATGG	1 cut(s)	68
NdeI	CATATG	2 cut(s)	1129, 1261
NgoMIV	GCCGGC	1 cut(s)	121
NlaIII	CATG	9 cut(s)	72, 595, 681, 1108, 1310, 1583, 1757, 2018, 2227
NlaIV	GGNNCC	8 cut(s)	46, 47, 454, 565, 615, 1209, 1436, 1815
NmeAIII	GCCGAG	1 cut(s)	177
NmuCI	GTSAC	2 cut(s)	82, 1509
NruI	TCGCGA	1 cut(s)	95
NsiI	ATGCAT	2 cut(s)	1951, 2066
NspI	RCATGY	1 cut(s)	1108
NspV	TTCGAA	2 cut(s)	1073, 1732
PagI	TCATGA	3 cut(s)	1579, 1753, 2014
PaqCI	CACCTGC	1 cut(s)	962
PasI	CCCWGGG	1 cut(s)	238
PciI	ACATGT	1 cut(s)	1104
PcsI	WCGNNNNNNNCGW	3 cut(s)	29, 86, 92
PctI	GAATGC	2 cut(s)	1119, 1676
PdiI	GCCGGC	1 cut(s)	123
PdmI	GAANNNNTTC	2 cut(s)	539, 1764
PfeI	GAWTC	3 cut(s)	1247, 1379, 1782
Pfl23II	CGTACG	1 cut(s)	126
PkrI	GCNGC	6 cut(s)	147, 150, 697, 955, 1701, 1909
Ple19I	CGATCG	1 cut(s)	36
PleI	GAGTC	4 cut(s)	343, 818, 1079, 1908
PpsI	GAGTC	4 cut(s)	343, 818, 1079, 1908
PpuMI	RGGWCCY	2 cut(s)	515, 1158
PscI	ACATGT	1 cut(s)	1104
Psp124BI	GAGCTC	2 cut(s)	59, 1374
Psp5II	RGGWCCY	2 cut(s)	515, 1158
Psp6I	CCWGG	1 cut(s)	237
PspFI	CCCAGC	2 cut(s)	168, 848
PspGI	CCWGG	1 cut(s)	237
PspLI	CGTACG	1 cut(s)	126
PspN4I	GGNNCC	8 cut(s)	46, 47, 454, 565, 615, 1209, 1436, 1815
PspOMI	GGGCCC	2 cut(s)	45, 452
PspPPI	RGGWCCY	2 cut(s)	515, 1158
PstI	CTGCAG	2 cut(s)	1701, 1794
PstNI	CAGNNNCTG	3 cut(s)	148, 698, 1658
PsuI	RGATCY	6 cut(s)	313, 644, 776, 946, 1207, 1434
PvuI	CGATCG	1 cut(s)	36
PvuII	CAGCTG	2 cut(s)	145, 698
RruI	TCGCGA	1 cut(s)	95
RsaI	GTAC	6 cut(s)	128, 565, 793, 1109, 1146, 1356
RsaNI	GTAC	6 cut(s)	127, 564, 792, 1108, 1145, 1355
RseI	CAYNNNNRTG	2 cut(s)	1188, 1447
Rsr2I	CGGWCCG	2 cut(s)	7, 907
RsrII	CGGWCCG	2 cut(s)	7, 907
SacI	GAGCTC	2 cut(s)	59, 1374
SaqAI	TTAA	2 cut(s)	417, 1419
SatI	GCNGC	6 cut(s)	146, 149, 696, 954, 1700, 1908
SchI	GAGTC	4 cut(s)	344, 819, 1079, 1909
ScrFI	CCNGG	3 cut(s)	43, 239, 456
SduI	GDGCHC	6 cut(s)	49, 59, 223, 456, 1374, 1810
SfaNI	GCATC	6 cut(s)	72, 892, 1936, 2051, 2073, 2095
SfcI	CTRYAG	5 cut(s)	327, 690, 940, 1697, 1790
SfuI	TTCGAA	2 cut(s)	1073, 1732
SgrAI	CRCCGGYG	1 cut(s)	121
SinI	GGWCC	6 cut(s)	7, 515, 634, 830, 907, 1158
SmiMI	CAYNNNNRTG	2 cut(s)	1188, 1447
SmlI	CTYRAG	1 cut(s)	1396
SmoI	CTYRAG	1 cut(s)	1396
Sse9I	AATT	8 cut(s)	401, 491, 573, 708, 1169, 1629, 2044, 2122
SsiI	CCGC	8 cut(s)	5, 25, 228, 729, 905, 1711, 2029, 2157
SspI	AATATT	1 cut(s)	1572
SspMI	CTAG	4 cut(s)	857, 888, 1535, 2216
SstI	GAGCTC	2 cut(s)	59, 1374
StyD4I	CCNGG	3 cut(s)	41, 237, 454
StyI	CCWWGG	4 cut(s)	68, 438, 772, 1290
TaiI	ACGT	4 cut(s)	188, 372, 871, 1958
TaqI	TCGA	5 cut(s)	36, 265, 1073, 1732, 2118
TasI	AATT	8 cut(s)	401, 491, 573, 708, 1169, 1629, 2044, 2122
TatI	WGTACW	3 cut(s)	791, 1144, 1354
TfiI	GAWTC	3 cut(s)	1247, 1379, 1782
Tru1I	TTAA	2 cut(s)	417, 1419
Tru9I	TTAA	2 cut(s)	417, 1419
TseFI	GTSAC	2 cut(s)	82, 1509
TseI	GCWGC	6 cut(s)	145, 148, 695, 953, 1699, 1907
Tsp45I	GTSAC	2 cut(s)	82, 1509
TspGWI	ACGGA	5 cut(s)	7, 144, 1378, 1736, 2166
VneI	GTGCAC	1 cut(s)	1806
VpaK11BI	GGWCC	6 cut(s)	7, 515, 634, 830, 907, 1158
XapI	RAATTY	2 cut(s)	401, 1169
XceI	RCATGY	1 cut(s)	1108
XcmI	CCANNNNNNNNNTGG	1 cut(s)	675
XmiI	GTMKAC	2 cut(s)	1010, 1044
XmnI	GAANNNNTTC	2 cut(s)	539, 1764
XspI	CTAG	4 cut(s)	857, 888, 1535, 2216
Zsp2I	ATGCAT	2 cut(s)	1951, 2066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.