AT2G29560

Cytosolic enolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014315
Physical Location & Seq
Forward (+)
12646560 .. 12650184
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G29560.1

Sequence Viewer

Length: 1428 bp
ATGTCTGTGCAAGAGTATTTAGACAAGCATATGCTTTCTCGGAAAATCGAAGACGCCGTTAATGCCGCCGTAAGGGCTAAAACCTCAGATCCCGTTCTCTTCATCGCTAATCATCTGAAGAAAGCTGTATCATCTGTGATAACGAAGGTTAAAGCACGACAGATCCTTGACAGCAGAGGAATTCCAACAGTTGAAGTTGATCTGCACACGAACAAAGGCGTTTTTCGTGCTTCTGTTCCCAGTGGTGATTCTTCTGGAACGTATGAAGCTATTGAGCTACGTGATGGAGACAAAGGAATGTATCTTGGAAACAGTGTGGCTAAAGCTGTTAAGAACATAAATGAAAAAATTTCTGAGGCGTTGATTGGTATGGACCCAAAACTTCAGGGTCAGATAGATCAGGCTATGATAGATTTGGATAAAACTGAAAAGAAGAGTGAACTTGGTGCTAATGCTATACTTGCTGTGTCAATTGCGGCATGCAAGGCTGGAGCTGCTGAGAAAGAGGTCCCTTTGTGCAAGCATCTTTCTGATCTTAGTGGCAGAGCAAACATGGTGTTACCTGTACCTGCGTTCACTGTTTTGAGTGGTGGGAAGCATGCTTCGAATACTTTTGCCATTCAGGAAATTATGATTCTCCCAATTGGAGCAAGTAGATTTGAGGAGGCCCTGCAATGGGGATCTGAGACATATCATCATTTAAAGGCTGTCATTTCAGAAAAGAATGGTGGTTTGGGATGTAATGTTGGTGAAGATGGTGGTCTTGCTCCAGATATCTCGAGCCTCAAGGAAGGTTTGGAGCTTGTAAAAGAAGCTATCAACCGAACAGGGTACAATGATAAGATAAAGATAGCCATTGATATTGCCGCCACTAATTTTTGTTTAGGTACCAAGTATGATTTAGATATCAAGTCTCCAAATAAATCTGGGCAAAATTTCAAGTCAGCGGAAGATATGATAGATATGTACAAAGAAATTTGTAATGATTATCCAATTGTGTCTATAGAAGACCCTTTTGACAAGGAGGACTGGGAACACACCAAGTATTTTTCGAGTCTTGGAATATGTCAGGTGGTAGGTGACGATTTGTTGATGTCAAATTCAAAACGAGTTGAGCGTGCCATACAGGAGTCTTCTTGTAATGCTCTTCTTCTCAAGGTGAATCAGATTGGTACAGTAACAGAAGCCATTGAAGTAGTGAAAATGGCAAGGGATGCCCAGTGGGGTGTGGTGACATCTCATAGATGTGGAGAAACAGAGGACTCTTTCATCTCTGACTTATCTGTGGGTCTCGCAACAGGTGTGATTAAAGCTGGTGCTCCTTGCAGAGGAGAACGTACTATGAAGTATAACCAGTTGCTACGGATAGAGGAAGAGCTTGGGGATCAAGCAGTGTATGCTGGAGAAGATTGGAAGCTATCTCTCTAA

Protein Analysis

475

Amino Acids

51.6

Weight (kDa)

5.34

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 47 - 176 2.8e-54 Enolase, N-terminal domain
Enolase_C PF00113 185 - 470 2.4e-118 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 315 - 417 1.2e-06 Enolase C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29560
fragaria_vesca FvH4_3g37690
malus_domestica MD03G1078500.v1.1
prunus_persica Prupe.6G063500_v2.0.a1
pyrus_communis pycom03g06290 pycom11g07120
rosa_chinensis RchiOBHm_Chr5g0067961
rosa_laevigata RLG00000035947
rosa_roxburghii Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa Rorug05G0386800
rosa_samantha Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 577
Acc65I GGTACC 1 cut(s) 887
AccB1I GGYRCC 1 cut(s) 887
AciI CCGC 4 cut(s) 66, 476, 867, 947
AclWI GGATC 4 cut(s) 83, 157, 688, 1392
AcsI RAATTY 5 cut(s) 180, 348, 934, 975, 1099
AcuI CTGAAG 2 cut(s) 137, 368
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 6 cut(s) 567, 833, 889, 968, 1174, 1339
AfiI CCNNNNNNNGG 4 cut(s) 72, 675, 676, 1328
AgsI TTSAA 4 cut(s) 194, 940, 1104, 1193
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
Alw21I GWGCWC 1 cut(s) 1321
Alw26I GTCTC 4 cut(s) 282, 680, 918, 1295
AlwI GGATC 4 cut(s) 83, 157, 688, 1392
Ama87I CYCGRG 1 cut(s) 778
AoxI GGCC 1 cut(s) 666
ApeKI GCWGC 1 cut(s) 494
ApoI RAATTY 5 cut(s) 180, 348, 934, 975, 1099
Asp718I GGTACC 1 cut(s) 887
AspS9I GGNCC 3 cut(s) 373, 508, 667
AsuHPI GGTGA 5 cut(s) 257, 761, 1091, 1171, 1243
AsuII TTCGAA 1 cut(s) 605
AvaI CYCGRG 1 cut(s) 778
AvaII GGWCC 2 cut(s) 373, 508
BanI GGYRCC 1 cut(s) 887
BbsI GAAGAC 3 cut(s) 57, 1014, 1125
Bbv12I GWGCWC 1 cut(s) 1321
BbvI GCAGC 1 cut(s) 481
BccI CCATC 2 cut(s) 278, 749
BceAI ACGGC 2 cut(s) 41, 53
BcoDI GTCTC 4 cut(s) 282, 680, 918, 1295
BfmI CTRYAG 1 cut(s) 1002
BfuAI ACCTGC 1 cut(s) 577
BglI GCCNNNNNGGC 1 cut(s) 74
BisI GCNGC 4 cut(s) 66, 477, 495, 867
BlsI GCNGC 4 cut(s) 67, 478, 496, 868
Bme18I GGWCC 2 cut(s) 373, 508
BmeT110I CYCGRG 1 cut(s) 778
BmgT120I GGNCC 3 cut(s) 373, 508, 667
BmiI GGNNCC 3 cut(s) 375, 510, 889
BmrI ACTGGG 3 cut(s) 234, 1039, 1213
BmsI GCATC 2 cut(s) 532, 1204
BmuI ACTGGG 3 cut(s) 234, 1039, 1213
BpiI GAAGAC 3 cut(s) 57, 1014, 1125
BpmI CTGGAG 3 cut(s) 510, 753, 1422
Bpu14I TTCGAA 1 cut(s) 605
BpuEI CTTGAG 2 cut(s) 770, 1139
BsaAI YACGTR 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 1295
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 675, 676, 1328
Bse1I ACTGG 4 cut(s) 240, 1034, 1219, 1354
Bse3DI GCAATG 1 cut(s) 680
BseGI GGATG 2 cut(s) 743, 1219
BseLI CCNNNNNNNGG 4 cut(s) 72, 675, 676, 1328
BseMI GCAATG 1 cut(s) 680
BseMII CTCAG 4 cut(s) 99, 345, 489, 675
BseNI ACTGG 4 cut(s) 240, 1034, 1219, 1354
BseRI GAGGAG 2 cut(s) 677, 1344
BseXI GCAGC 1 cut(s) 481
BsgI GTGCAG 1 cut(s) 188
BshFI GGCC 1 cut(s) 668
BshNI GGYRCC 1 cut(s) 887
BsiHKAI GWGCWC 1 cut(s) 1321
BsiHKCI CYCGRG 1 cut(s) 778
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 4 cut(s) 72, 675, 676, 1328
BsmAI GTCTC 4 cut(s) 282, 680, 918, 1295
BsmFI GGGAC 1 cut(s) 494
BsnI GGCC 1 cut(s) 668
Bso31I GGTCTC 1 cut(s) 1295
BsoBI CYCGRG 1 cut(s) 778
Bsp119I TTCGAA 1 cut(s) 605
Bsp1286I GDGCHC 1 cut(s) 1321
Bsp1407I TGTACA 1 cut(s) 966
Bsp143I GATC 7 cut(s) 88, 162, 199, 397, 532, 680, 1384
BspACI CCGC 4 cut(s) 66, 476, 867, 947
BspANI GGCC 1 cut(s) 668
BspCNI CTCAG 4 cut(s) 98, 346, 490, 676
BspLI GGNNCC 3 cut(s) 375, 510, 889
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 4 cut(s) 83, 157, 688, 1392
BspQI GCTCTTC 2 cut(s) 1152, 1368
BspT104I TTCGAA 1 cut(s) 605
BspT107I GGYRCC 1 cut(s) 887
BspTNI GGTCTC 1 cut(s) 1295
BsrDI GCAATG 1 cut(s) 680
BsrGI TGTACA 1 cut(s) 966
BsrI ACTGG 4 cut(s) 240, 1034, 1219, 1354
BssMI GATC 7 cut(s) 88, 162, 199, 397, 532, 680, 1384
BssNI GRCGYC 1 cut(s) 54
Bst4CI ACNGT 4 cut(s) 190, 314, 580, 1177
Bst6I CTCTTC 4 cut(s) 104, 428, 1152, 1368
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 2 cut(s) 1214, 1397
BstAUI TGTACA 1 cut(s) 966
BstBAI YACGTR 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 605
BstC8I GCNNGC 4 cut(s) 481, 521, 600, 1119
BstDEI CTNAG 5 cut(s) 85, 354, 498, 536, 684
BstENI CCTNNNNNAGG 1 cut(s) 1326
BstF5I GGATG 2 cut(s) 743, 1219
BstKTI GATC 7 cut(s) 91, 165, 202, 400, 535, 683, 1387
BstMAI GTCTC 4 cut(s) 282, 680, 918, 1295
BstMBI GATC 7 cut(s) 88, 162, 199, 397, 532, 680, 1384
BstMWI GCNNNNNNNGC 7 cut(s) 62, 74, 461, 485, 494, 1214, 1397
BstNSI RCATGY 2 cut(s) 483, 602
BstSFI CTRYAG 1 cut(s) 1002
BstV1I GCAGC 1 cut(s) 481
BstV2I GAAGAC 3 cut(s) 57, 1014, 1125
BstX2I RGATCY 3 cut(s) 88, 162, 680
BstYI RGATCY 3 cut(s) 88, 162, 680
BsuRI GGCC 1 cut(s) 668
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 2 cut(s) 743, 1219
BtsI GCAGTG 1 cut(s) 1398
BtsIMutI CAGTG 5 cut(s) 247, 319, 576, 1226, 1398
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 4 cut(s) 481, 521, 600, 1119
Cfr13I GGNCC 3 cut(s) 373, 508, 667
CseI GACGC 1 cut(s) 62
Csp6I GTAC 6 cut(s) 566, 832, 888, 967, 1173, 1338
CviAII CATG 3 cut(s) 480, 553, 599
CviQI GTAC 6 cut(s) 566, 832, 888, 967, 1173, 1338
DdeI CTNAG 5 cut(s) 85, 354, 498, 536, 684
DpnI GATC 7 cut(s) 90, 164, 201, 399, 534, 682, 1386
DpnII GATC 7 cut(s) 88, 162, 199, 397, 532, 680, 1384
DraI TTTAAA 1 cut(s) 702
Eam1104I CTCTTC 4 cut(s) 104, 428, 1152, 1368
EarI CTCTTC 4 cut(s) 104, 428, 1152, 1368
Eco31I GGTCTC 1 cut(s) 1295
Eco32I GATATC 2 cut(s) 775, 907
Eco47I GGWCC 2 cut(s) 373, 508
Eco57I CTGAAG 2 cut(s) 137, 368
Eco88I CYCGRG 1 cut(s) 778
EcoNI CCTNNNNNAGG 1 cut(s) 1326
EcoO109I RGGNCCY 2 cut(s) 508, 667
EcoRI GAATTC 1 cut(s) 180
EcoRV GATATC 2 cut(s) 775, 907
FaeI CATG 3 cut(s) 483, 556, 602
FaqI GGGAC 1 cut(s) 494
FatI CATG 3 cut(s) 479, 552, 598
FauNDI CATATG 1 cut(s) 30
Fnu4HI GCNGC 4 cut(s) 66, 477, 495, 867
FokI GGATG 2 cut(s) 750, 1226
Fsp4HI GCNGC 4 cut(s) 66, 477, 495, 867
GluI GCNGC 4 cut(s) 66, 477, 495, 867
GsuI CTGGAG 3 cut(s) 510, 753, 1422
HaeIII GGCC 1 cut(s) 668
HgaI GACGC 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 3 cut(s) 483, 556, 602
HinfI GANTC 6 cut(s) 248, 634, 1054, 1130, 1162, 1262
HphI GGTGA 5 cut(s) 257, 761, 1091, 1171, 1243
Hpy166II GTNNAC 2 cut(s) 440, 576
Hpy188III TCNNGA 4 cut(s) 255, 623, 770, 778
Hpy8I GTNNAC 2 cut(s) 440, 576
HpyAV CCTTC 2 cut(s) 139, 785
HpyCH4III ACNGT 4 cut(s) 190, 314, 580, 1177
HpyCH4IV ACGT 3 cut(s) 260, 280, 1336
HpyCH4V TGCA 6 cut(s) 10, 205, 483, 519, 673, 1326
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 74, 461, 485, 494, 1214, 1397
HpyF3I CTNAG 5 cut(s) 85, 354, 498, 536, 684
HpySE526I ACGT 3 cut(s) 260, 280, 1336
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 3 cut(s) 483, 556, 602
KpnI GGTACC 1 cut(s) 891
Kzo9I GATC 7 cut(s) 88, 162, 199, 397, 532, 680, 1384
LguI GCTCTTC 2 cut(s) 1152, 1368
LmnI GCTCC 5 cut(s) 491, 647, 772, 799, 1324
Lsp1109I GCAGC 1 cut(s) 481
LweI GCATC 2 cut(s) 532, 1204
MaeII ACGT 3 cut(s) 260, 280, 1336
MaeIII GTNAC 4 cut(s) 558, 1079, 1177, 1231
MalI GATC 7 cut(s) 90, 164, 201, 399, 534, 682, 1386
MboI GATC 7 cut(s) 88, 162, 199, 397, 532, 680, 1384
MfeI CAATTG 3 cut(s) 471, 642, 993
MflI RGATCY 3 cut(s) 88, 162, 680
MhlI GDGCHC 1 cut(s) 1321
MlyI GAGTC 3 cut(s) 1063, 1139, 1256
MmeI TCCRAC 1 cut(s) 209
MseI TTAA 5 cut(s) 60, 150, 330, 701, 1308
MslI CAYNNNNRTG 1 cut(s) 1245
MspA1I CMGCKG 1 cut(s) 947
MunI CAATTG 3 cut(s) 471, 642, 993
MwoI GCNNNNNNNGC 7 cut(s) 62, 74, 461, 485, 494, 1214, 1397
NdeI CATATG 1 cut(s) 30
NdeII GATC 7 cut(s) 88, 162, 199, 397, 532, 680, 1384
NlaIII CATG 3 cut(s) 483, 556, 602
NlaIV GGNNCC 3 cut(s) 375, 510, 889
NmuCI GTSAC 2 cut(s) 1079, 1231
NspI RCATGY 2 cut(s) 483, 602
NspV TTCGAA 1 cut(s) 605
PaeI GCATGC 2 cut(s) 483, 602
PaeR7I CTCGAG 1 cut(s) 778
PciSI GCTCTTC 2 cut(s) 1152, 1368
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 1114
PfeI GAWTC 3 cut(s) 248, 634, 1162
PkrI GCNGC 4 cut(s) 67, 478, 496, 868
PleI GAGTC 3 cut(s) 1062, 1138, 1256
PpsI GAGTC 3 cut(s) 1062, 1138, 1256
Ppu21I YACGTR 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 508
PspN4I GGNNCC 3 cut(s) 375, 510, 889
PspPI GGNCC 3 cut(s) 373, 508, 667
PspPPI RGGWCCY 1 cut(s) 508
PsuI RGATCY 3 cut(s) 88, 162, 680
RsaI GTAC 6 cut(s) 567, 833, 889, 968, 1174, 1339
RsaNI GTAC 6 cut(s) 566, 832, 888, 967, 1173, 1338
RseI CAYNNNNRTG 1 cut(s) 1245
SapI GCTCTTC 2 cut(s) 1152, 1368
SaqAI TTAA 5 cut(s) 60, 150, 330, 701, 1308
SatI GCNGC 4 cut(s) 66, 477, 495, 867
Sau3AI GATC 7 cut(s) 88, 162, 199, 397, 532, 680, 1384
Sau96I GGNCC 3 cut(s) 373, 508, 667
SchI GAGTC 3 cut(s) 1063, 1139, 1256
SduI GDGCHC 1 cut(s) 1321
SfaNI GCATC 2 cut(s) 532, 1204
SfcI CTRYAG 1 cut(s) 1002
Sfr274I CTCGAG 1 cut(s) 778
SfuI TTCGAA 1 cut(s) 605
SinI GGWCC 2 cut(s) 373, 508
SlaI CTCGAG 1 cut(s) 778
SmiMI CAYNNNNRTG 1 cut(s) 1245
SmlI CTYRAG 3 cut(s) 778, 785, 1154
SmoI CTYRAG 3 cut(s) 778, 785, 1154
SphI GCATGC 2 cut(s) 483, 602
SsiI CCGC 4 cut(s) 66, 476, 867, 947
TaaI ACNGT 4 cut(s) 190, 314, 580, 1177
TaiI ACGT 3 cut(s) 263, 283, 1339
TaqI TCGA 4 cut(s) 48, 605, 779, 1052
TatI WGTACW 1 cut(s) 966
TauI GCSGC 3 cut(s) 68, 479, 869
TfiI GAWTC 3 cut(s) 248, 634, 1162
Tru1I TTAA 5 cut(s) 60, 150, 330, 701, 1308
Tru9I TTAA 5 cut(s) 60, 150, 330, 701, 1308
TscAI CASTG 5 cut(s) 247, 319, 583, 1226, 1398
TseFI GTSAC 2 cut(s) 1079, 1231
TseI GCWGC 1 cut(s) 494
Tsp45I GTSAC 2 cut(s) 1079, 1231
TspDTI ATGAA 5 cut(s) 91, 279, 357, 1258, 1358
TspGWI ACGGA 1 cut(s) 1378
TspRI CASTG 5 cut(s) 247, 319, 583, 1226, 1398
VpaK11BI GGWCC 2 cut(s) 373, 508
XagI CCTNNNNNAGG 1 cut(s) 1326
XapI RAATTY 5 cut(s) 180, 348, 934, 975, 1099
XceI RCATGY 2 cut(s) 483, 602
XhoI CTCGAG 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.