Rroxscaffold_1G00013350

Cytosolic enolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014315
Physical Location & Seq
Reverse (-)
16402144 .. 16407276
5133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00013350.1

Sequence Viewer

Length: 1278 bp
ATGTCGGTCCAAGAGTATCTGGATAATCACTTGCTCTCTCGCAAAATCGAAGACGCCGTCAATGCCGCCGTCAGGGCCAAAGCCGTCGATCCCGTCCTCTTCATCTCGAATCATATGAGGAAGGCGATTCCCTCAGTGATAACGAAGGTCAAAGCTCGGCAGATCCTCGATAGCAGAGGAATTCCTACTGTTGAAGTTGATCTTCATACTAACAAGGGCATGTTTCGTGCTTCAGCTCCGAGTTCCGATCTTACTGGATTTGGTGAAACTAGGTATGACGCTGTTGAATTGCGAGATGGGGATAAGGGATCGTATCTTGGAAGTAGTGTGAATAAAGCTGTTAAGAATGTGAATGAGAAAATATCTGAAGCGTTGGTTGGGATGGATCCTACGCTTCAATCGCAGATTGATCAGGCAATGATAGACTTGGATAAGACAGAAAAGAAGGTTCCACTGTACAAACACATTGCTGACCTTTCTGGAAGAAACACCCTGACCCTGCCTGTTCCTGCTTTCACTCTTATAAGTGGAGGAAAGCGTGCTGGGAATAGTCTAGCCATTCAGGAAATTATGATTCTCCCAATTGGAGCTAACAGGTTTGAGGAGGCACTACAAATGGGATCAGAAACTTATCATCACTTGAAGGCTGTTATTGTAGACAAGTATGGCGCACATGGATGTAATGTCGGTGAAGACGGTGGTTTTGCTCCCAACATCTCCAGGCAAGTCTGTCTTAGTACGAAGTATGACCTGGACTACAAATCACCGAATAAGTCAGGGCAAAACTTTAAGTCGGCAGAAGATATGATCAAGTTGTATAGAGAACTTTGTACTGAGTACCCAATTGTATCAATTGAAGATCCGTTTGATAAGGAGGACTGGGAACACACCAAACGCTTTTCTAATCTTGCAATTTGTCAGGTAGTTGGAGATGACTTGTTAATGTCAAATTCACAACGTATGAAGAAAGCAATAGAGGAATCTACGTGTGATGCTCTTCTTCTGAAGATTAACCAGATTGGGACAGTGACTGAGGCCATTGAAGTGGTTAAACTGGCAAAGGATGCCCACTGGGGAGTTGTGGCATCTCATAGATGTGGTGAAACTGAAGAGTCCTTTATAGCTGACTTCTCTGTTGGTCTCTCTACTGGTCAAATCAAAGCTGGTGCTCCTTGCAGAAGCGAGCGACTGGCTAAGTATAACCAGTTGCTTCGAATTGAGGAAGAGCTTGGGGATGACGCATTTTATGCTGGTGAAGAGTGGAGGGAATCACCCTGA

Protein Analysis

425

Amino Acids

46.94

Weight (kDa)

5.51

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 47 - 150 5.1e-33 Enolase, N-terminal domain
Enolase_C PF00113 165 - 241 7e-27 Enolase, C-terminal TIM barrel domain
Enolase_C PF00113 247 - 422 5.1e-73 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 265 - 387 1.2e-08 Enolase C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29560
fragaria_vesca FvH4_3g37690
malus_domestica MD03G1078500.v1.1
prunus_persica Prupe.6G063500_v2.0.a1
pyrus_communis pycom03g06290 pycom11g07120
rosa_chinensis RchiOBHm_Chr5g0067961
rosa_laevigata RLG00000035947
rosa_roxburghii Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa Rorug05G0386800
rosa_samantha Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AccI GTMKAC 1 cut(s) 657
AciI CCGC 1 cut(s) 66
AclWI GGATC 7 cut(s) 83, 157, 316, 380, 393, 628, 854
AcsI RAATTY 2 cut(s) 180, 949
AcuI CTGAAG 4 cut(s) 216, 387, 1025, 1128
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 4 cut(s) 458, 739, 832, 839
AfiI CCNNNNNNNGG 1 cut(s) 72
AflIII ACRYGT 1 cut(s) 986
AgsI TTSAA 6 cut(s) 194, 287, 398, 643, 857, 1043
AjnI CCWGG 2 cut(s) 719, 750
AjuI GAANNNNNNNTTGG 2 cut(s) 360, 392
AluBI AGCT 7 cut(s) 155, 236, 338, 590, 1124, 1163, 1228
AluI AGCT 7 cut(s) 155, 236, 338, 590, 1124, 1163, 1228
Alw21I GWGCWC 1 cut(s) 1171
Alw26I GTCTC 1 cut(s) 1145
AlwI GGATC 7 cut(s) 83, 157, 316, 380, 393, 628, 854
AlwNI CAGNNNCTG 1 cut(s) 1031
AoxI GGCC 2 cut(s) 75, 1035
ApoI RAATTY 2 cut(s) 180, 949
ArsI GACNNNNNNTTYG 4 cut(s) 42, 74, 686, 718
AspLEI GCGC 1 cut(s) 671
AspS9I GGNCC 2 cut(s) 7, 75
AsuHPI GGTGA 6 cut(s) 275, 701, 756, 1112, 1263, 1265
AsuII TTCGAA 1 cut(s) 1213
AvaII GGWCC 1 cut(s) 7
BamHI GGATCC 1 cut(s) 385
BbsI GAAGAC 2 cut(s) 57, 699
Bbv12I GWGCWC 1 cut(s) 1171
BccI CCATC 2 cut(s) 290, 376
BceAI ACGGC 3 cut(s) 41, 53, 68
BciT130I CCWGG 2 cut(s) 721, 752
BclI TGATCA 2 cut(s) 409, 807
BcoDI GTCTC 1 cut(s) 1145
BfaI CTAG 2 cut(s) 270, 554
BglI GCCNNNNNGGC 1 cut(s) 74
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 721, 752
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 7, 75
BmiI GGNNCC 2 cut(s) 387, 450
BmrFI CCNGG 2 cut(s) 721, 752
BmrI ACTGGG 2 cut(s) 889, 1081
BmsI GCATC 3 cut(s) 982, 1054, 1094
BmuI ACTGGG 2 cut(s) 889, 1081
BpiI GAAGAC 2 cut(s) 57, 699
BpmI CTGGAG 1 cut(s) 703
Bpu14I TTCGAA 1 cut(s) 1213
BsaAI YACGTR 1 cut(s) 987
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 1145
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse1I ACTGG 7 cut(s) 259, 884, 1059, 1076, 1153, 1194, 1204
Bse3DI GCAATG 2 cut(s) 423, 465
BseBI CCWGG 2 cut(s) 721, 752
BseGI GGATG 4 cut(s) 387, 683, 1069, 1240
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMI GCAATG 2 cut(s) 423, 465
BseMII CTCAG 3 cut(s) 147, 825, 1023
BseNI ACTGG 7 cut(s) 259, 884, 1059, 1076, 1153, 1194, 1204
BseRI GAGGAG 1 cut(s) 617
BseYI CCCAGC 1 cut(s) 542
BshFI GGCC 2 cut(s) 77, 1037
BsiHKAI GWGCWC 1 cut(s) 1171
BslFI GGGAC 1 cut(s) 1036
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 1 cut(s) 1145
BsmFI GGGAC 1 cut(s) 1036
BsnI GGCC 2 cut(s) 77, 1037
Bso31I GGTCTC 1 cut(s) 1145
Bsp119I TTCGAA 1 cut(s) 1213
Bsp1286I GDGCHC 1 cut(s) 1171
Bsp1407I TGTACA 1 cut(s) 456
BspACI CCGC 1 cut(s) 66
BspANI GGCC 2 cut(s) 77, 1037
BspCNI CTCAG 3 cut(s) 146, 826, 1024
BspLI GGNNCC 2 cut(s) 387, 450
BspPI GGATC 7 cut(s) 83, 157, 316, 380, 393, 628, 854
BspQI GCTCTTC 2 cut(s) 1002, 1218
BspT104I TTCGAA 1 cut(s) 1213
BspTNI GGTCTC 1 cut(s) 1145
BsrDI GCAATG 2 cut(s) 423, 465
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 7 cut(s) 259, 884, 1059, 1076, 1153, 1194, 1204
BssNI GRCGYC 1 cut(s) 54
Bst2UI CCWGG 2 cut(s) 721, 752
Bst4CI ACNGT 4 cut(s) 190, 456, 698, 1027
Bst6I CTCTTC 5 cut(s) 104, 1002, 1104, 1218, 1251
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 2 cut(s) 1064, 1247
BstAUI TGTACA 1 cut(s) 456
BstBAI YACGTR 1 cut(s) 987
BstBI TTCGAA 1 cut(s) 1213
BstC8I GCNNGC 2 cut(s) 540, 1184
BstDEI CTNAG 5 cut(s) 133, 734, 834, 1032, 1194
BstF5I GGATG 4 cut(s) 387, 683, 1069, 1240
BstHHI GCGC 1 cut(s) 671
BstMAI GTCTC 1 cut(s) 1145
BstMWI GCNNNNNNNGC 5 cut(s) 62, 74, 400, 1064, 1247
BstNI CCWGG 2 cut(s) 721, 752
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 2 cut(s) 719, 750
BstV2I GAAGAC 2 cut(s) 57, 699
BstX2I RGATCY 3 cut(s) 162, 385, 859
BstXI CCANNNNNNTGG 1 cut(s) 1045
BstYI RGATCY 3 cut(s) 162, 385, 859
BsuRI GGCC 2 cut(s) 77, 1037
BtsCI GGATG 4 cut(s) 387, 683, 1069, 1240
BtsIMutI CAGTG 4 cut(s) 141, 452, 1032, 1069
Cac8I GCNNGC 2 cut(s) 540, 1184
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 1 cut(s) 671
Cfr13I GGNCC 2 cut(s) 7, 75
CseI GACGC 3 cut(s) 62, 287, 1247
Csp6I GTAC 4 cut(s) 457, 738, 831, 838
CviAII CATG 2 cut(s) 220, 674
CviQI GTAC 4 cut(s) 457, 738, 831, 838
DdeI CTNAG 5 cut(s) 133, 734, 834, 1032, 1194
Eam1104I CTCTTC 5 cut(s) 104, 1002, 1104, 1218, 1251
EarI CTCTTC 5 cut(s) 104, 1002, 1104, 1218, 1251
Eco31I GGTCTC 1 cut(s) 1145
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 4 cut(s) 216, 387, 1025, 1128
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 719, 750
FaeI CATG 2 cut(s) 223, 677
FalI AAGNNNNNCTT 4 cut(s) 186, 218, 717, 749
FaqI GGGAC 1 cut(s) 1036
FatI CATG 2 cut(s) 219, 673
FauNDI CATATG 1 cut(s) 114
FbaI TGATCA 2 cut(s) 409, 807
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 4 cut(s) 394, 690, 1076, 1247
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 2 cut(s) 270, 554
GlaI GCGC 1 cut(s) 670
GluI GCNGC 1 cut(s) 66
GsaI CCCAGC 1 cut(s) 546
GsuI CTGGAG 1 cut(s) 703
HaeIII GGCC 2 cut(s) 77, 1037
HgaI GACGC 3 cut(s) 62, 287, 1247
HhaI GCGC 1 cut(s) 671
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 2 cut(s) 223, 677
Hin6I GCGC 1 cut(s) 669
HinP1I GCGC 1 cut(s) 669
HinfI GANTC 6 cut(s) 109, 127, 574, 980, 1112, 1268
HphI GGTGA 6 cut(s) 275, 701, 756, 1112, 1263, 1265
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 5 cut(s) 240, 247, 367, 625, 1005
Hpy188III TCNNGA 4 cut(s) 20, 106, 480, 563
Hpy8I GTNNAC 1 cut(s) 658
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 4 cut(s) 115, 139, 439, 637
HpyCH4III ACNGT 4 cut(s) 190, 456, 698, 1027
HpyCH4IV ACGT 2 cut(s) 958, 986
HpyCH4V TGCA 2 cut(s) 911, 1176
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 74, 400, 1064, 1247
HpyF3I CTNAG 5 cut(s) 133, 734, 834, 1032, 1194
HpySE526I ACGT 2 cut(s) 958, 986
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 2 cut(s) 223, 677
HspAI GCGC 1 cut(s) 669
Ksp22I TGATCA 2 cut(s) 409, 807
LguI GCTCTTC 2 cut(s) 1002, 1218
LmnI GCTCC 4 cut(s) 241, 587, 712, 1174
LweI GCATC 3 cut(s) 982, 1054, 1094
MaeI CTAG 2 cut(s) 270, 554
MaeII ACGT 2 cut(s) 958, 986
MaeIII GTNAC 1 cut(s) 1027
MfeI CAATTG 3 cut(s) 582, 843, 852
MflI RGATCY 3 cut(s) 162, 385, 859
MhlI GDGCHC 1 cut(s) 1171
MluCI AATT 9 cut(s) 180, 287, 567, 582, 843, 852, 912, 949, 1215
MlyI GAGTC 1 cut(s) 1121
MmeI TCCRAC 1 cut(s) 907
MseI TTAA 5 cut(s) 342, 789, 941, 1011, 1050
MslI CAYNNNNRTG 3 cut(s) 676, 1043, 1095
MspR9I CCNGG 2 cut(s) 721, 752
MunI CAATTG 3 cut(s) 582, 843, 852
MvaI CCWGG 2 cut(s) 721, 752
MwoI GCNNNNNNNGC 5 cut(s) 62, 74, 400, 1064, 1247
NdeI CATATG 1 cut(s) 114
NlaIII CATG 2 cut(s) 223, 677
NlaIV GGNNCC 2 cut(s) 387, 450
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 1027
NspI RCATGY 1 cut(s) 223
NspV TTCGAA 1 cut(s) 1213
PciSI GCTCTTC 2 cut(s) 1002, 1218
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 5 cut(s) 109, 127, 574, 980, 1268
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 1 cut(s) 1120
PpsI GAGTC 1 cut(s) 1120
Ppu21I YACGTR 1 cut(s) 987
PsiI TTATAA 1 cut(s) 524
Psp6I CCWGG 2 cut(s) 719, 750
PspFI CCCAGC 1 cut(s) 542
PspGI CCWGG 2 cut(s) 719, 750
PspN4I GGNNCC 2 cut(s) 387, 450
PspPI GGNCC 2 cut(s) 7, 75
PstNI CAGNNNCTG 1 cut(s) 1031
PsuI RGATCY 3 cut(s) 162, 385, 859
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 4 cut(s) 458, 739, 832, 839
RsaNI GTAC 4 cut(s) 457, 738, 831, 838
RseI CAYNNNNRTG 3 cut(s) 676, 1043, 1095
SapI GCTCTTC 2 cut(s) 1002, 1218
SaqAI TTAA 5 cut(s) 342, 789, 941, 1011, 1050
SatI GCNGC 1 cut(s) 66
Sau96I GGNCC 2 cut(s) 7, 75
SchI GAGTC 1 cut(s) 1121
ScrFI CCNGG 2 cut(s) 721, 752
SduI GDGCHC 1 cut(s) 1171
SfaNI GCATC 3 cut(s) 982, 1054, 1094
SfuI TTCGAA 1 cut(s) 1213
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 3 cut(s) 676, 1043, 1095
Sse9I AATT 9 cut(s) 180, 287, 567, 582, 843, 852, 912, 949, 1215
SsiI CCGC 1 cut(s) 66
SspMI CTAG 2 cut(s) 270, 554
StyD4I CCNGG 2 cut(s) 719, 750
TaaI ACNGT 4 cut(s) 190, 456, 698, 1027
TaiI ACGT 2 cut(s) 961, 989
TaqI TCGA 5 cut(s) 48, 87, 107, 168, 1213
TasI AATT 9 cut(s) 180, 287, 567, 582, 843, 852, 912, 949, 1215
TatI WGTACW 2 cut(s) 456, 830
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 5 cut(s) 109, 127, 574, 980, 1268
Tru1I TTAA 5 cut(s) 342, 789, 941, 1011, 1050
Tru9I TTAA 5 cut(s) 342, 789, 941, 1011, 1050
TscAI CASTG 4 cut(s) 141, 459, 1032, 1076
TseFI GTSAC 1 cut(s) 1027
Tsp45I GTSAC 1 cut(s) 1027
TspDTI ATGAA 3 cut(s) 91, 194, 977
TspGWI ACGGA 1 cut(s) 852
TspRI CASTG 4 cut(s) 141, 459, 1032, 1076
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 2 cut(s) 180, 949
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 657
XspI CTAG 2 cut(s) 270, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.