Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0014315

Physical Location & Seq

Genomic Coordinates Reverse (-)

6351612 .. 6357418

Gene Structure 5807 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1078500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1401 bp

ATGTCGGTGCAAGATTACTTGGATAACCACATGCTCTCTCGGAAAATCGAAGACGCCGTCAATGCCGCCGTTAGGGCTAAGGCCTCCGATCCCGTCCTCTTCATCTCGAATCATATGAAGAAAGCGGTACCTTCAGTGATAACAAAGGTCAAAGCTCGGCAGATCCTCGATAGCAGAGGAATTCCAACTGTTGAAGTCGACCTCCACACTAACAAAGGAACGTTTCGTGCTTCAGCTCCTAGCTCTGATGTTTCCGGAATGTATGAGGCTGTTGAACTGCGAGATGGGGACAAGGGATTATATCTTGGAAATAGTGTGACTAAAGCTGTTAAGAATGTCAATGAGAAAATATCTGAAGCATTGGTAGGCATGGATCCTACGCTTCAGTCGCAGATTGATCAGGCAATGATAGACCTCGATAAAACAGAAAAGAAGAGTGAACTTGGAGTAAATGCCATATTGGCTGTTCCACTGTACAAACATATTGCTGACCTTTCTGGAAAAGGCCACCTGACCCTGCCTGTCCCTGCTTTTACTGTTATTAGTGGAGGAAAGCATGCTGGGAATAATCTGGCCATTCAGGAAATTATGATTCTCCCAATTGGAGCTAGCAAGTTTGAGGAGGCATTGCAAATGGGATCTGAAACCTATCATCACCTGAAGCAGGCTGTTATTACAGAAAAATATGGCTCACATGGATGTAATGTTGGTGAAGATGGTGGTTTTGCTCCCAACATCTCGAGGCAAGTATTTATTGTCGTTAGAGAAGTCTTGGATCTTGTAAAAGAGGCGATCAGTAGAACAGGTTATAATGAGCAAATCAAACTAGCAATTGATTTTGCTGCTACTGACTTTTGCATAGGTACAAAGTATGACCTGGATTACAAATCAGCGGATAAGGGACAAAATTTCAAGTCGGCTGATGATATGATCGAGATGTATAAAGAACTTTGTAGTGAGTACCAAATTGTGTCAATTGAAGATCCATTTGATAAAGAGGACTGGGAACACACTAAACGCTTTTCTAGCCTTGGAATTTGTCAGGTAGTCGGAGATGACTTGATAACATCAAATCCAAAGCGTATTAAGAGAGCTATAGAGGAGTCCACCTGCAATGCTCTACTTGTAAAGATAAACCAGATTGGAACAGTGACCGAGGCCATTGAAGTGATGAAGCTGGCAAAGGATGCCCACTGGGGAGTGGTCATGTCTCATAGATGTGGTGAAACGGAAGATTCCTTCATAGCTGACTTAGCTGTTGGTCTCTCAACGGGTCAGATCAAAGCTGGTGCTCCTTGCAGAGGCGAACGGCTGGCCAAGTATAACCAGTTACTTCGAATTGAGGAAGAACTTGGGGACGATGCATTTTATGCTGGTGAGGATTGGAGGCAATCATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

467

Amino Acids

51.17

Weight (kDa)

5.36

Isoelectric Point (pI)

40.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	47 - 158	3.3e-42	Enolase, N-terminal domain
Enolase_C	PF00113	171 - 464	1.7e-117	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	306 - 429	4.1e-10	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014315)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29560
fragaria_vesca	FvH4_3g37690
malus_domestica	MD03G1078500.v1.1
prunus_persica	Prupe.6G063500_v2.0.a1
pyrus_communis	pycom03g06290 pycom11g07120
rosa_chinensis	RchiOBHm_Chr5g0067961
rosa_laevigata	RLG00000035947
rosa_roxburghii	Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa	Rorug05G0386800
rosa_samantha	Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana	Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	810
AarI	CACCTGC	1 cut(s)	1118
Acc36I	ACCTGC	1 cut(s)	1118
Acc65I	GGTACC	1 cut(s)	127
AccB1I	GGYRCC	1 cut(s)	127
AccI	GTMKAC	1 cut(s)	198
AccIII	TCCGGA	1 cut(s)	254
AciI	CCGC	3 cut(s)	66, 125, 893
AclI	AACGTT	1 cut(s)	221
AclWI	GGATC	7 cut(s)	83, 157, 368, 381, 646, 783, 977
AcoI	YGGCCR	2 cut(s)	573, 1314
AcsI	RAATTY	3 cut(s)	180, 907, 1035
AcuI	CTGAAG	5 cut(s)	117, 216, 368, 375, 680
AcyI	GRCGYC	1 cut(s)	54
AfaI	GTAC	4 cut(s)	129, 476, 865, 962
AfiI	CCNNNNNNNGG	3 cut(s)	72, 664, 1301
AgsI	TTSAA	5 cut(s)	194, 275, 913, 980, 1166
AjnI	CCWGG	1 cut(s)	876
Alw21I	GWGCWC	1 cut(s)	1294
Alw26I	GTCTC	2 cut(s)	1215, 1268
AlwI	GGATC	7 cut(s)	83, 157, 368, 381, 646, 783, 977
Ama87I	CYCGRG	1 cut(s)	739
Aor13HI	TCCGGA	1 cut(s)	254
AoxI	GGCC	5 cut(s)	81, 505, 573, 1158, 1314
ApeKI	GCWGC	1 cut(s)	842
ApoI	RAATTY	3 cut(s)	180, 907, 1035
ArsI	GACNNNNNNTTYG	2 cut(s)	42, 74
Asp718I	GGTACC	1 cut(s)	127
AsuHPI	GGTGA	4 cut(s)	647, 722, 1235, 1388
AsuII	TTCGAA	1 cut(s)	1336
AsuNHI	GCTAGC	1 cut(s)	608
AvaI	CYCGRG	1 cut(s)	739
BalI	TGGCCA	2 cut(s)	575, 1316
BamHI	GGATCC	1 cut(s)	373
BanI	GGYRCC	1 cut(s)	127
BbsI	GAAGAC	1 cut(s)	57
Bbv12I	GWGCWC	1 cut(s)	1294
BbvI	GCAGC	1 cut(s)	829
BccI	CCATC	2 cut(s)	278, 710
BceAI	ACGGC	3 cut(s)	41, 53, 1325
BciT130I	CCWGG	1 cut(s)	878
BclI	TGATCA	1 cut(s)	397
BcoDI	GTCTC	2 cut(s)	1215, 1268
BfaI	CTAG	4 cut(s)	240, 609, 827, 1026
BfmI	CTRYAG	1 cut(s)	1095
BfuAI	ACCTGC	1 cut(s)	1118
BglI	GCCNNNNNGGC	2 cut(s)	74, 461
BisI	GCNGC	2 cut(s)	66, 843
BlsI	GCNGC	2 cut(s)	67, 844
Bme1390I	CCNGG	1 cut(s)	878
BmeT110I	CYCGRG	1 cut(s)	739
BmiI	GGNNCC	2 cut(s)	129, 375
BmrFI	CCNGG	1 cut(s)	878
BmrI	ACTGGG	2 cut(s)	1012, 1204
BmsI	GCATC	2 cut(s)	1177, 1351
BmtI	GCTAGC	1 cut(s)	612
BmuI	ACTGGG	2 cut(s)	1012, 1204
BpiI	GAAGAC	1 cut(s)	57
Bpu10I	CCTNAGC	1 cut(s)	78
Bpu14I	TTCGAA	1 cut(s)	1336
BsaHI	GRCGYC	1 cut(s)	54
BsaI	GGTCTC	1 cut(s)	1268
BsaJI	CCNNGG	2 cut(s)	1030, 1155
BsaWI	WCCGGW	1 cut(s)	254
Bsc4I	CCNNNNNNNGG	3 cut(s)	72, 664, 1301
Bse1I	ACTGG	3 cut(s)	1007, 1199, 1327
Bse3DI	GCAATG	3 cut(s)	411, 626, 1120
BseAI	TCCGGA	1 cut(s)	254
BseBI	CCWGG	1 cut(s)	878
BseDI	CCNNGG	2 cut(s)	1030, 1155
BseGI	GGATG	3 cut(s)	704, 1192, 1394
BseLI	CCNNNNNNNGG	3 cut(s)	72, 664, 1301
BseMI	GCAATG	3 cut(s)	411, 626, 1120
BseNI	ACTGG	3 cut(s)	1007, 1199, 1327
BseRI	GAGGAG	2 cut(s)	635, 1115
BseXI	GCAGC	1 cut(s)	829
BseYI	CCCAGC	1 cut(s)	560
BshFI	GGCC	5 cut(s)	83, 507, 575, 1160, 1316
BshNI	GGYRCC	1 cut(s)	127
BsiHKAI	GWGCWC	1 cut(s)	1294
BsiHKCI	CYCGRG	1 cut(s)	739
BsiSI	CCGG	1 cut(s)	255
BslFI	GGGAC	4 cut(s)	302, 509, 915, 1370
BslI	CCNNNNNNNGG	3 cut(s)	72, 664, 1301
BsmAI	GTCTC	2 cut(s)	1215, 1268
BsmFI	GGGAC	4 cut(s)	302, 509, 915, 1370
BsnI	GGCC	5 cut(s)	83, 507, 575, 1160, 1316
Bso31I	GGTCTC	1 cut(s)	1268
BsoBI	CYCGRG	1 cut(s)	739
Bsp119I	TTCGAA	1 cut(s)	1336
Bsp1286I	GDGCHC	1 cut(s)	1294
Bsp13I	TCCGGA	1 cut(s)	254
Bsp1407I	TGTACA	1 cut(s)	474
BspACI	CCGC	3 cut(s)	66, 125, 893
BspANI	GGCC	5 cut(s)	83, 507, 575, 1160, 1316
BspEI	TCCGGA	1 cut(s)	254
BspLI	GGNNCC	2 cut(s)	129, 375
BspMI	ACCTGC	1 cut(s)	1118
BspOI	GCTAGC	1 cut(s)	612
BspPI	GGATC	7 cut(s)	83, 157, 368, 381, 646, 783, 977
BspT104I	TTCGAA	1 cut(s)	1336
BspT107I	GGYRCC	1 cut(s)	127
BspTNI	GGTCTC	1 cut(s)	1268
BsrDI	GCAATG	3 cut(s)	411, 626, 1120
BsrGI	TGTACA	1 cut(s)	474
BsrI	ACTGG	3 cut(s)	1007, 1199, 1327
BssECI	CCNNGG	2 cut(s)	1030, 1155
BssNI	GRCGYC	1 cut(s)	54
BssT1I	CCWWGG	1 cut(s)	1030
Bst2UI	CCWGG	1 cut(s)	878
Bst4CI	ACNGT	4 cut(s)	190, 474, 538, 1150
Bst6I	CTCTTC	2 cut(s)	104, 428
BstACI	GRCGYC	1 cut(s)	54
BstAPI	GCANNNNNTGC	2 cut(s)	1187, 1370
BstAUI	TGTACA	1 cut(s)	474
BstBI	TTCGAA	1 cut(s)	1336
BstC8I	GCNNGC	5 cut(s)	558, 610, 666, 1179, 1314
BstDEI	CTNAG	2 cut(s)	78, 1252
BstENI	CCTNNNNNAGG	2 cut(s)	662, 1299
BstF5I	GGATG	3 cut(s)	704, 1192, 1394
BstMAI	GTCTC	2 cut(s)	1215, 1268
BstMWI	GCNNNNNNNGC	8 cut(s)	62, 74, 388, 461, 1026, 1187, 1253, 1370
BstNI	CCWGG	1 cut(s)	878
BstNSI	RCATGY	2 cut(s)	34, 560
BstSCI	CCNGG	1 cut(s)	876
BstSFI	CTRYAG	1 cut(s)	1095
BstV1I	GCAGC	1 cut(s)	829
BstV2I	GAAGAC	1 cut(s)	57
BstX2I	RGATCY	5 cut(s)	162, 373, 638, 775, 982
BstYI	RGATCY	5 cut(s)	162, 373, 638, 775, 982
BsuRI	GGCC	5 cut(s)	83, 507, 575, 1160, 1316
BtsCI	GGATG	3 cut(s)	704, 1192, 1394
BtsIMutI	CAGTG	4 cut(s)	141, 470, 1155, 1192
BveI	ACCTGC	1 cut(s)	1118
Cac8I	GCNNGC	5 cut(s)	558, 610, 666, 1179, 1314
CseI	GACGC	1 cut(s)	62
Csp6I	GTAC	4 cut(s)	128, 475, 864, 961
CviAII	CATG	5 cut(s)	31, 370, 557, 695, 1207
CviQI	GTAC	4 cut(s)	128, 475, 864, 961
DdeI	CTNAG	2 cut(s)	78, 1252
EaeI	YGGCCR	2 cut(s)	573, 1314
Eam1104I	CTCTTC	2 cut(s)	104, 428
EarI	CTCTTC	2 cut(s)	104, 428
Eco130I	CCWWGG	1 cut(s)	1030
Eco147I	AGGCCT	1 cut(s)	83
Eco31I	GGTCTC	1 cut(s)	1268
Eco57I	CTGAAG	5 cut(s)	117, 216, 368, 375, 680
Eco88I	CYCGRG	1 cut(s)	739
EcoNI	CCTNNNNNAGG	2 cut(s)	662, 1299
EcoRI	GAATTC	1 cut(s)	180
EcoRII	CCWGG	1 cut(s)	876
EcoT14I	CCWWGG	1 cut(s)	1030
EcoT22I	ATGCAT	1 cut(s)	1366
ErhI	CCWWGG	1 cut(s)	1030
FaeI	CATG	5 cut(s)	34, 373, 560, 698, 1210
FaqI	GGGAC	4 cut(s)	302, 509, 915, 1370
FatI	CATG	5 cut(s)	30, 369, 556, 694, 1206
FauNDI	CATATG	1 cut(s)	114
FbaI	TGATCA	1 cut(s)	397
FblI	GTMKAC	1 cut(s)	198
Fnu4HI	GCNGC	2 cut(s)	66, 843
FokI	GGATG	3 cut(s)	711, 1199, 1381
Fsp4HI	GCNGC	2 cut(s)	66, 843
FspBI	CTAG	4 cut(s)	240, 609, 827, 1026
GluI	GCNGC	2 cut(s)	66, 843
GsaI	CCCAGC	1 cut(s)	564
HaeIII	GGCC	5 cut(s)	83, 507, 575, 1160, 1316
HapII	CCGG	1 cut(s)	255
HgaI	GACGC	1 cut(s)	62
Hin1I	GRCGYC	1 cut(s)	54
Hin1II	CATG	5 cut(s)	34, 373, 560, 698, 1210
HincII	GTYRAC	1 cut(s)	199
HindII	GTYRAC	1 cut(s)	199
HinfI	GANTC	4 cut(s)	109, 592, 1103, 1235
HpaII	CCGG	1 cut(s)	255
HphI	GGTGA	4 cut(s)	647, 722, 1235, 1388
Hpy166II	GTNNAC	3 cut(s)	199, 440, 1107
Hpy188I	TCNGA	7 cut(s)	42, 88, 247, 355, 643, 1052, 1278
Hpy188III	TCNNGA	7 cut(s)	106, 255, 498, 581, 739, 934, 1398
Hpy8I	GTNNAC	3 cut(s)	199, 440, 1107
HpyAV	CCTTC	2 cut(s)	141, 1249
HpyCH4III	ACNGT	4 cut(s)	190, 474, 538, 1150
HpyCH4IV	ACGT	1 cut(s)	221
HpyCH4V	TGCA	6 cut(s)	10, 631, 858, 1113, 1299, 1364
HpyF10VI	GCNNNNNNNGC	8 cut(s)	62, 74, 388, 461, 1026, 1187, 1253, 1370
HpyF3I	CTNAG	2 cut(s)	78, 1252
HpySE526I	ACGT	1 cut(s)	221
Hsp92I	GRCGYC	1 cut(s)	54
Hsp92II	CATG	5 cut(s)	34, 373, 560, 698, 1210
Kpn2I	TCCGGA	1 cut(s)	254
KpnI	GGTACC	1 cut(s)	131
Ksp22I	TGATCA	1 cut(s)	397
LmnI	GCTCC	4 cut(s)	241, 605, 733, 1297
Lsp1109I	GCAGC	1 cut(s)	829
LweI	GCATC	2 cut(s)	1177, 1351
MaeI	CTAG	4 cut(s)	240, 609, 827, 1026
MaeII	ACGT	1 cut(s)	221
MaeIII	GTNAC	3 cut(s)	316, 1150, 1329
MboII	GAAGA	8 cut(s)	62, 91, 130, 445, 725, 992, 1244, 1358
MfeI	CAATTG	3 cut(s)	600, 831, 975
MflI	RGATCY	5 cut(s)	162, 373, 638, 775, 982
MhlI	GDGCHC	1 cut(s)	1294
MlsI	TGGCCA	2 cut(s)	575, 1316
MluCI	AATT	9 cut(s)	180, 585, 600, 831, 907, 966, 975, 1035, 1338
MluNI	TGGCCA	2 cut(s)	575, 1316
MlyI	GAGTC	1 cut(s)	1112
MmeI	TCCRAC	2 cut(s)	209, 1030
Mox20I	TGGCCA	2 cut(s)	575, 1316
Mph1103I	ATGCAT	1 cut(s)	1366
MroI	TCCGGA	1 cut(s)	254
MscI	TGGCCA	2 cut(s)	575, 1316
MseI	TTAA	2 cut(s)	330, 1086
MslI	CAYNNNNRTG	3 cut(s)	697, 1166, 1218
Msp20I	TGGCCA	2 cut(s)	575, 1316
MspA1I	CMGCKG	1 cut(s)	893
MspI	CCGG	1 cut(s)	255
MspR9I	CCNGG	1 cut(s)	878
MunI	CAATTG	3 cut(s)	600, 831, 975
MvaI	CCWGG	1 cut(s)	878
MwoI	GCNNNNNNNGC	8 cut(s)	62, 74, 388, 461, 1026, 1187, 1253, 1370
NdeI	CATATG	1 cut(s)	114
NheI	GCTAGC	1 cut(s)	608
NlaIII	CATG	5 cut(s)	34, 373, 560, 698, 1210
NlaIV	GGNNCC	2 cut(s)	129, 375
NmeAIII	GCCGAG	1 cut(s)	136
NmuCI	GTSAC	2 cut(s)	316, 1150
NsiI	ATGCAT	1 cut(s)	1366
NspI	RCATGY	2 cut(s)	34, 560
NspV	TTCGAA	1 cut(s)	1336
PaeI	GCATGC	1 cut(s)	560
PaeR7I	CTCGAG	1 cut(s)	739
PaqCI	CACCTGC	1 cut(s)	1118
PceI	AGGCCT	1 cut(s)	83
PcsI	WCGNNNNNNNCGW	1 cut(s)	54
PfeI	GAWTC	3 cut(s)	109, 592, 1235
PflFI	GACNNNGTC	1 cut(s)	56
PkrI	GCNGC	2 cut(s)	67, 844
PleI	GAGTC	1 cut(s)	1111
PpsI	GAGTC	1 cut(s)	1111
PsiI	TTATAA	1 cut(s)	810
Psp1406I	AACGTT	1 cut(s)	221
Psp6I	CCWGG	1 cut(s)	876
PspFI	CCCAGC	1 cut(s)	560
PspGI	CCWGG	1 cut(s)	876
PspN4I	GGNNCC	2 cut(s)	129, 375
PsuI	RGATCY	5 cut(s)	162, 373, 638, 775, 982
PsyI	GACNNNGTC	1 cut(s)	56
RsaI	GTAC	4 cut(s)	129, 476, 865, 962
RsaNI	GTAC	4 cut(s)	128, 475, 864, 961
RseI	CAYNNNNRTG	3 cut(s)	697, 1166, 1218
SalI	GTCGAC	1 cut(s)	197
SaqAI	TTAA	2 cut(s)	330, 1086
SatI	GCNGC	2 cut(s)	66, 843
SchI	GAGTC	1 cut(s)	1112
ScrFI	CCNGG	1 cut(s)	878
SduI	GDGCHC	1 cut(s)	1294
SfaNI	GCATC	2 cut(s)	1177, 1351
SfcI	CTRYAG	1 cut(s)	1095
Sfr274I	CTCGAG	1 cut(s)	739
SfuI	TTCGAA	1 cut(s)	1336
SlaI	CTCGAG	1 cut(s)	739
SmiMI	CAYNNNNRTG	3 cut(s)	697, 1166, 1218
SmlI	CTYRAG	1 cut(s)	739
SmoI	CTYRAG	1 cut(s)	739
SphI	GCATGC	1 cut(s)	560
Sse9I	AATT	9 cut(s)	180, 585, 600, 831, 907, 966, 975, 1035, 1338
SseBI	AGGCCT	1 cut(s)	83
SsiI	CCGC	3 cut(s)	66, 125, 893
SspMI	CTAG	4 cut(s)	240, 609, 827, 1026
StuI	AGGCCT	1 cut(s)	83
StyD4I	CCNGG	1 cut(s)	876
StyI	CCWWGG	1 cut(s)	1030
TaaI	ACNGT	4 cut(s)	190, 474, 538, 1150
TaiI	ACGT	1 cut(s)	224
TaqI	TCGA	8 cut(s)	48, 107, 168, 198, 417, 740, 933, 1336
TaqII	GACCGA	1 cut(s)	1169
TasI	AATT	9 cut(s)	180, 585, 600, 831, 907, 966, 975, 1035, 1338
TatI	WGTACW	1 cut(s)	474
TauI	GCSGC	1 cut(s)	68
TfiI	GAWTC	3 cut(s)	109, 592, 1235
Tru1I	TTAA	2 cut(s)	330, 1086
Tru9I	TTAA	2 cut(s)	330, 1086
TscAI	CASTG	4 cut(s)	141, 477, 1155, 1199
TseFI	GTSAC	2 cut(s)	316, 1150
TseI	GCWGC	1 cut(s)	842
Tsp45I	GTSAC	2 cut(s)	316, 1150
TspDTI	ATGAA	4 cut(s)	91, 131, 1187, 1231
TspGWI	ACGGA	1 cut(s)	1244
TspRI	CASTG	4 cut(s)	141, 477, 1155, 1199
Tth111I	GACNNNGTC	1 cut(s)	56
XagI	CCTNNNNNAGG	2 cut(s)	662, 1299
XapI	RAATTY	3 cut(s)	180, 907, 1035
XceI	RCATGY	2 cut(s)	34, 560
XhoI	CTCGAG	1 cut(s)	739
XmiI	GTMKAC	1 cut(s)	198
XspI	CTAG	4 cut(s)	240, 609, 827, 1026
Zsp2I	ATGCAT	1 cut(s)	1366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.