RLG00000035947

Cytosolic enolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014315
Physical Location & Seq
Forward (+)
73923665 .. 73929060
5396 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035947

Sequence Viewer

Length: 1440 bp
ATGTCGGTCCAAGAGTATCTGGATAATCACTTGCTCTCTCGCAAAATCGAAGACGCCGTCAATGCCGCCGTCAGGGCCAAAGCCGCCGATCCCGTCCTGTTCATCTCGAATCATATGAGGAAGGCGATTCCCTCAGTGATAACGAAGGTCAAAGCTCGGCAGATCCTTGATAGCAGAGGAATTCCTACTGTTGAAGTTGATCTTCATACTAACAAGGGCATGTTTCGTGCTTCAGCTCCGAGTTCCGATCTCACTGGATTTGGTGAAACTAGGTATGAGGCTGTTGAGTTGCGAGATGGGGATAAGGGATCGTATCTCGGAAGTAGTGTGAATAAAGCTGTTAAGAATGTGAATGAGAAAATATCTGAAGCCTTGGTTGGGATGGATCCTACGCTTCAATCACAGATTGATCAGGCAATGATAGACTTGGATAAGACAGAAAAGAAGGGTGAACTTGGAGTGAATGCCATATTGGCTGTGTCGATGGCTGCTTGCAAAGCTGGGGCTGCTCAGAAAGAGGTTCCACTGTACAAACACATTGCTGACCTTTCTGGAAGAAACACCCTGACCCTGCCTGTTCCTGCTTTCACTCTTATAAGTGGAGGAAAGCGTGCTGGGAATAGTCTAGCCATTCAGGAAATTATGATTCTCCCAATTGGAGCTAACAGGTTTGAGGAGGCACTACAAATGGGATCAGAAACTTATCATCACTTGAAGGCTGTTATTGTAGACAAGTATGGCGCACATGGATGTAATGTTGGTGAAGACGGTGGTTTTGCTCCCAACATCTCCAGTTTTAGAGAAGGCTTGGATCTTGTAAAAGAGGCTATCAGCAGAACAGGCTATAATGACCGGATAAAAATAGCAATTGATGTTGCTGCTACGAACTTCTGCATGGGTACGAAGTATGACCTGGACTACAAATCACCGAATAAGTCAGGGCAAAACTTTAAGTCGGCAGAAGATATGATTAAGTTGTATAGAGAACTATGTACTGAGTACCCAATTGTATCAATTGAAGACCCGTTTGATAAGGAGGACTGGGAACACACCAAAAGCTTTTCTAATCTTGCAATTTGTCAGGTAGTTGGAGATGATTTGTTAATGTCAAATTCACAACGTATGAAGAAAGCGATAGAGGAATCTACGTGTGATGCTCTTCTTCTGAAGATTAACCAGATTGGGACAGTGACTGAGGCCATTGAAGTGGTTAAACTGGCAAAGGATGCCCACTGGGGAGTTGTTGCATCTCATAGATGTGGTGAAACTGAAGAGTCCTTTATAGCTGACTTCTCTGTTGGTCTCTCTACTGGTCAAATCAAAGCTGGTGCTCCTTGTAGGAGCGAGCGACTGGCTAAGTATAACCAGTTGCTTCGAATTGAGGAAGAGCTTGGGGACGATGCATTTTATGCTGGTGAAGAGTGGAGGGAATCACCCTGA

Protein Analysis

480

Amino Acids

52.42

Weight (kDa)

5.4

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 47 - 180 6.1e-50 Enolase, N-terminal domain
Enolase_C PF00113 189 - 476 2.8e-119 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 319 - 441 2.5e-08 Enolase C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29560
fragaria_vesca FvH4_3g37690
malus_domestica MD03G1078500.v1.1
prunus_persica Prupe.6G063500_v2.0.a1
pyrus_communis pycom03g06290 pycom11g07120
rosa_chinensis RchiOBHm_Chr5g0067961
rosa_laevigata RLG00000035947
rosa_roxburghii Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa Rorug05G0386800
rosa_samantha Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 596
AccI GTMKAC 1 cut(s) 729
AciI CCGC 2 cut(s) 66, 84
AclWI GGATC 7 cut(s) 83, 157, 316, 380, 393, 700, 819
AcsI RAATTY 2 cut(s) 180, 1111
AcuI CTGAAG 4 cut(s) 216, 387, 1187, 1290
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 4 cut(s) 530, 901, 994, 1001
AfiI CCNNNNNNNGG 2 cut(s) 72, 378
AflIII ACRYGT 1 cut(s) 1148
AgsI TTSAA 5 cut(s) 194, 398, 715, 1019, 1205
AjnI CCWGG 1 cut(s) 912
AjuI GAANNNNNNNTTGG 4 cut(s) 360, 392, 455, 487
AluBI AGCT 9 cut(s) 155, 236, 338, 500, 662, 1059, 1286, 1325, 1390
AluI AGCT 9 cut(s) 155, 236, 338, 500, 662, 1059, 1286, 1325, 1390
Alw21I GWGCWC 1 cut(s) 1333
Alw26I GTCTC 1 cut(s) 1307
AlwI GGATC 7 cut(s) 83, 157, 316, 380, 393, 700, 819
AlwNI CAGNNNCTG 1 cut(s) 1193
AoxI GGCC 2 cut(s) 75, 1197
ApeKI GCWGC 3 cut(s) 488, 506, 878
ApoI RAATTY 2 cut(s) 180, 1111
ArsI GACNNNNNNTTYG 4 cut(s) 42, 74, 758, 790
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 2 cut(s) 7, 75
AsuHPI GGTGA 7 cut(s) 275, 461, 773, 918, 1274, 1425, 1427
AsuII TTCGAA 1 cut(s) 1375
AvaII GGWCC 1 cut(s) 7
BamHI GGATCC 1 cut(s) 385
BbsI GAAGAC 3 cut(s) 57, 771, 1026
Bbv12I GWGCWC 1 cut(s) 1333
BbvI GCAGC 3 cut(s) 475, 493, 865
BccI CCATC 3 cut(s) 290, 376, 478
BceAI ACGGC 2 cut(s) 41, 53
BciT130I CCWGG 1 cut(s) 914
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 1307
BfaI CTAG 2 cut(s) 270, 626
BglI GCCNNNNNGGC 2 cut(s) 74, 473
BisI GCNGC 5 cut(s) 66, 84, 489, 507, 879
BlsI GCNGC 5 cut(s) 67, 85, 490, 508, 880
Bme1390I CCNGG 1 cut(s) 914
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 7, 75
BmiI GGNNCC 2 cut(s) 387, 522
BmrFI CCNGG 1 cut(s) 914
BmrI ACTGGG 2 cut(s) 1051, 1243
BmsI GCATC 4 cut(s) 1144, 1216, 1256, 1390
BmuI ACTGGG 2 cut(s) 1051, 1243
BpiI GAAGAC 3 cut(s) 57, 771, 1026
BpmI CTGGAG 1 cut(s) 775
Bpu14I TTCGAA 1 cut(s) 1375
BsaAI YACGTR 1 cut(s) 1149
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 1307
BsaJI CCNNGG 1 cut(s) 372
BsaWI WCCGGW 1 cut(s) 852
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 378
Bse1I ACTGG 8 cut(s) 259, 792, 1046, 1221, 1238, 1315, 1356, 1366
Bse3DI GCAATG 2 cut(s) 423, 537
BseBI CCWGG 1 cut(s) 914
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 3 cut(s) 387, 755, 1231
BseLI CCNNNNNNNGG 2 cut(s) 72, 378
BseMI GCAATG 2 cut(s) 423, 537
BseMII CTCAG 4 cut(s) 147, 524, 987, 1185
BseNI ACTGG 8 cut(s) 259, 792, 1046, 1221, 1238, 1315, 1356, 1366
BseRI GAGGAG 1 cut(s) 689
BseXI GCAGC 3 cut(s) 475, 493, 865
BseYI CCCAGC 2 cut(s) 500, 614
BshFI GGCC 2 cut(s) 77, 1199
BsiHKAI GWGCWC 1 cut(s) 1333
BsiSI CCGG 1 cut(s) 853
BslFI GGGAC 2 cut(s) 1198, 1409
BslI CCNNNNNNNGG 2 cut(s) 72, 378
BsmAI GTCTC 1 cut(s) 1307
BsmFI GGGAC 2 cut(s) 1198, 1409
BsmI GAATGC 1 cut(s) 469
BsnI GGCC 2 cut(s) 77, 1199
Bso31I GGTCTC 1 cut(s) 1307
Bsp119I TTCGAA 1 cut(s) 1375
Bsp1286I GDGCHC 1 cut(s) 1333
Bsp1407I TGTACA 1 cut(s) 528
Bsp143I GATC 9 cut(s) 88, 162, 199, 247, 308, 385, 409, 692, 811
BspACI CCGC 2 cut(s) 66, 84
BspANI GGCC 2 cut(s) 77, 1199
BspCNI CTCAG 4 cut(s) 146, 523, 988, 1186
BspLI GGNNCC 2 cut(s) 387, 522
BspPI GGATC 7 cut(s) 83, 157, 316, 380, 393, 700, 819
BspQI GCTCTTC 2 cut(s) 1164, 1380
BspT104I TTCGAA 1 cut(s) 1375
BspTNI GGTCTC 1 cut(s) 1307
BsrDI GCAATG 2 cut(s) 423, 537
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 8 cut(s) 259, 792, 1046, 1221, 1238, 1315, 1356, 1366
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 9 cut(s) 88, 162, 199, 247, 308, 385, 409, 692, 811
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 4 cut(s) 190, 528, 770, 1189
Bst6I CTCTTC 4 cut(s) 1164, 1266, 1380, 1413
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 2 cut(s) 1226, 1409
BstAUI TGTACA 1 cut(s) 528
BstBAI YACGTR 1 cut(s) 1149
BstBI TTCGAA 1 cut(s) 1375
BstC8I GCNNGC 3 cut(s) 493, 612, 1346
BstDEI CTNAG 5 cut(s) 133, 510, 996, 1194, 1356
BstF5I GGATG 3 cut(s) 387, 755, 1231
BstHHI GCGC 1 cut(s) 743
BstKTI GATC 9 cut(s) 91, 165, 202, 250, 311, 388, 412, 695, 814
BstMAI GTCTC 1 cut(s) 1307
BstMBI GATC 9 cut(s) 88, 162, 199, 247, 308, 385, 409, 692, 811
BstMWI GCNNNNNNNGC 9 cut(s) 62, 74, 83, 473, 497, 506, 840, 1226, 1409
BstNI CCWGG 1 cut(s) 914
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 912
BstV1I GCAGC 3 cut(s) 475, 493, 865
BstV2I GAAGAC 3 cut(s) 57, 771, 1026
BstX2I RGATCY 3 cut(s) 162, 385, 811
BstXI CCANNNNNNTGG 1 cut(s) 1207
BstYI RGATCY 3 cut(s) 162, 385, 811
BsuRI GGCC 2 cut(s) 77, 1199
BtsCI GGATG 3 cut(s) 387, 755, 1231
BtsIMutI CAGTG 5 cut(s) 141, 252, 524, 1194, 1231
Cac8I GCNNGC 3 cut(s) 493, 612, 1346
CaiI CAGNNNCTG 1 cut(s) 1193
CfoI GCGC 1 cut(s) 743
Cfr13I GGNCC 2 cut(s) 7, 75
CseI GACGC 1 cut(s) 62
Csp6I GTAC 4 cut(s) 529, 900, 993, 1000
CviAII CATG 3 cut(s) 220, 746, 895
CviQI GTAC 4 cut(s) 529, 900, 993, 1000
DdeI CTNAG 5 cut(s) 133, 510, 996, 1194, 1356
DpnI GATC 9 cut(s) 90, 164, 201, 249, 310, 387, 411, 694, 813
DpnII GATC 9 cut(s) 88, 162, 199, 247, 308, 385, 409, 692, 811
Eam1104I CTCTTC 4 cut(s) 1164, 1266, 1380, 1413
EarI CTCTTC 4 cut(s) 1164, 1266, 1380, 1413
Eco130I CCWWGG 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 1307
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 4 cut(s) 216, 387, 1187, 1290
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 912
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 1405
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 3 cut(s) 223, 749, 898
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 2 cut(s) 1198, 1409
FatI CATG 3 cut(s) 219, 745, 894
FauNDI CATATG 1 cut(s) 114
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 1 cut(s) 729
Fnu4HI GCNGC 5 cut(s) 66, 84, 489, 507, 879
FokI GGATG 3 cut(s) 394, 762, 1238
Fsp4HI GCNGC 5 cut(s) 66, 84, 489, 507, 879
FspBI CTAG 2 cut(s) 270, 626
GlaI GCGC 1 cut(s) 742
GluI GCNGC 5 cut(s) 66, 84, 489, 507, 879
GsaI CCCAGC 2 cut(s) 504, 618
GsuI CTGGAG 1 cut(s) 775
HaeIII GGCC 2 cut(s) 77, 1199
HapII CCGG 1 cut(s) 853
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 743
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 3 cut(s) 223, 749, 898
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HindIII AAGCTT 1 cut(s) 1057
HinfI GANTC 6 cut(s) 109, 127, 646, 1142, 1274, 1430
HpaII CCGG 1 cut(s) 853
HphI GGTGA 7 cut(s) 275, 461, 773, 918, 1274, 1425, 1427
Hpy166II GTNNAC 2 cut(s) 452, 730
Hpy188I TCNGA 7 cut(s) 240, 247, 320, 367, 513, 697, 1167
Hpy188III TCNNGA 4 cut(s) 20, 106, 552, 635
Hpy8I GTNNAC 2 cut(s) 452, 730
HpyAV CCTTC 5 cut(s) 115, 139, 439, 709, 797
HpyCH4III ACNGT 4 cut(s) 190, 528, 770, 1189
HpyCH4IV ACGT 2 cut(s) 1120, 1148
HpyCH4V TGCA 5 cut(s) 495, 894, 1073, 1247, 1403
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 74, 83, 473, 497, 506, 840, 1226, 1409
HpyF3I CTNAG 5 cut(s) 133, 510, 996, 1194, 1356
HpySE526I ACGT 2 cut(s) 1120, 1148
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 3 cut(s) 223, 749, 898
HspAI GCGC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 9 cut(s) 88, 162, 199, 247, 308, 385, 409, 692, 811
LguI GCTCTTC 2 cut(s) 1164, 1380
LmnI GCTCC 5 cut(s) 241, 659, 784, 1336, 1341
Lsp1109I GCAGC 3 cut(s) 475, 493, 865
LweI GCATC 4 cut(s) 1144, 1216, 1256, 1390
MaeI CTAG 2 cut(s) 270, 626
MaeII ACGT 2 cut(s) 1120, 1148
MaeIII GTNAC 1 cut(s) 1189
MalI GATC 9 cut(s) 90, 164, 201, 249, 310, 387, 411, 694, 813
MboI GATC 9 cut(s) 88, 162, 199, 247, 308, 385, 409, 692, 811
MfeI CAATTG 4 cut(s) 654, 867, 1005, 1014
MflI RGATCY 3 cut(s) 162, 385, 811
MhlI GDGCHC 1 cut(s) 1333
MluCI AATT 9 cut(s) 180, 639, 654, 867, 1005, 1014, 1074, 1111, 1377
MlyI GAGTC 1 cut(s) 1283
MmeI TCCRAC 1 cut(s) 1069
Mph1103I ATGCAT 1 cut(s) 1405
MseI TTAA 6 cut(s) 342, 951, 972, 1103, 1173, 1212
MslI CAYNNNNRTG 3 cut(s) 748, 1205, 1257
MspI CCGG 1 cut(s) 853
MspR9I CCNGG 1 cut(s) 914
MunI CAATTG 4 cut(s) 654, 867, 1005, 1014
Mva1269I GAATGC 1 cut(s) 469
MvaI CCWGG 1 cut(s) 914
MwoI GCNNNNNNNGC 9 cut(s) 62, 74, 83, 473, 497, 506, 840, 1226, 1409
NdeI CATATG 1 cut(s) 114
NdeII GATC 9 cut(s) 88, 162, 199, 247, 308, 385, 409, 692, 811
NlaIII CATG 3 cut(s) 223, 749, 898
NlaIV GGNNCC 2 cut(s) 387, 522
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 1189
NsiI ATGCAT 1 cut(s) 1405
NspI RCATGY 1 cut(s) 223
NspV TTCGAA 1 cut(s) 1375
PciSI GCTCTTC 2 cut(s) 1164, 1380
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 469
PfeI GAWTC 5 cut(s) 109, 127, 646, 1142, 1430
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 5 cut(s) 67, 85, 490, 508, 880
PleI GAGTC 1 cut(s) 1282
PpsI GAGTC 1 cut(s) 1282
Ppu21I YACGTR 1 cut(s) 1149
PsiI TTATAA 1 cut(s) 596
Psp6I CCWGG 1 cut(s) 912
PspFI CCCAGC 2 cut(s) 500, 614
PspGI CCWGG 1 cut(s) 912
PspN4I GGNNCC 2 cut(s) 387, 522
PspPI GGNCC 2 cut(s) 7, 75
PstNI CAGNNNCTG 1 cut(s) 1193
PsuI RGATCY 3 cut(s) 162, 385, 811
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 4 cut(s) 530, 901, 994, 1001
RsaNI GTAC 4 cut(s) 529, 900, 993, 1000
RseI CAYNNNNRTG 3 cut(s) 748, 1205, 1257
SapI GCTCTTC 2 cut(s) 1164, 1380
SaqAI TTAA 6 cut(s) 342, 951, 972, 1103, 1173, 1212
SatI GCNGC 5 cut(s) 66, 84, 489, 507, 879
Sau3AI GATC 9 cut(s) 88, 162, 199, 247, 308, 385, 409, 692, 811
Sau96I GGNCC 2 cut(s) 7, 75
SchI GAGTC 1 cut(s) 1283
ScrFI CCNGG 1 cut(s) 914
SduI GDGCHC 1 cut(s) 1333
SfaNI GCATC 4 cut(s) 1144, 1216, 1256, 1390
SfuI TTCGAA 1 cut(s) 1375
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 3 cut(s) 748, 1205, 1257
Sse9I AATT 9 cut(s) 180, 639, 654, 867, 1005, 1014, 1074, 1111, 1377
SsiI CCGC 2 cut(s) 66, 84
SspMI CTAG 2 cut(s) 270, 626
StyD4I CCNGG 1 cut(s) 912
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 4 cut(s) 190, 528, 770, 1189
TaiI ACGT 2 cut(s) 1123, 1151
TaqI TCGA 4 cut(s) 48, 107, 482, 1375
TasI AATT 9 cut(s) 180, 639, 654, 867, 1005, 1014, 1074, 1111, 1377
TatI WGTACW 2 cut(s) 528, 992
TauI GCSGC 2 cut(s) 68, 86
TfiI GAWTC 5 cut(s) 109, 127, 646, 1142, 1430
Tru1I TTAA 6 cut(s) 342, 951, 972, 1103, 1173, 1212
Tru9I TTAA 6 cut(s) 342, 951, 972, 1103, 1173, 1212
TscAI CASTG 5 cut(s) 141, 259, 531, 1194, 1238
TseFI GTSAC 1 cut(s) 1189
TseI GCWGC 3 cut(s) 488, 506, 878
Tsp45I GTSAC 1 cut(s) 1189
TspDTI ATGAA 3 cut(s) 91, 194, 1139
TspRI CASTG 5 cut(s) 141, 259, 531, 1194, 1238
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 2 cut(s) 180, 1111
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 729
XspI CTAG 2 cut(s) 270, 626
Zsp2I ATGCAT 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.