Rh5CG485100

Cytosolic enolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014315
Physical Location & Seq
Forward (+)
67705000 .. 67709593
4594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG485100.1

Sequence Viewer

Length: 1440 bp
ATGTCGGTCCAAGAGTATCTGGATAATCACTTGCTCTCTCGCAAAATCGAAGACGCCGTCAATGCCGCCGTCAGGGCCAAAGCCGCTGATCCCGTCCTCTTCATCTCCAATCATATGAGGAAGGCGATTCCCTCAGTGATAACGAAGGTCAAAGCTCGGCAGATCCTCGATAGCAGAGGCATTCCTACTGTTGAAGTTGATCTTCATACTAACAAGGGCATGTTTCGTGCTTCAGCTCCGAGTTCCGATCTAACTGGATTTGGTGAAACTAGGTATGAGGCTGTTGAATTGCGAGATGGGGATAAGGGATCGTATCTCGGAAGTAGTGTGAATAAAGCTGTTAAGAATGTGAATGAGAAAATATCTGAAGCATTGGTTGGGATGGATCCTACGCTTCAATCGCAGATTGATCAGGCAATGATAGACTTGGATAAGACAGAAAAGAAGGGTGAACTTGGAGTAAATGCCATATTGGCTGTGTCAATGGCTGCTTGCAAAGCTGGGGCTGCTCAGAAAGAGGTTCCACTGTACAAACACATTGCTGACCTTTCTGGAAGAAACACCCTGACCCTGCCTGTTCCTGCTTTCACTCTTATAAGTGGAGGAAAGCGTGCTGGGAATAGTCTAGCCATTCAGGAAATTATGATTCTCCCAATTGGAGCTAACAGGTTTGCGGAGGCACTACAAATGGGATCAGAAACTTATCATCACTTGAAGGCTGTTATTGTAGACAAGTATGGCGCACATGGATGTAATGTCGGTGAAGATGGTGGTTTTGCTCCCAACATCTCCAGTTTTAGAGAAGGCTTGGATCTTGTAAAAGAGGCTATCAGCAGAACAGGCTATAATGACCGGATAAAAATAGCAATTGATGTTGCTGCTACGAACTTCTGCATGGGTACAAAGTATGACCTGGACTACAAATCACCGAATAAGTCAGGGCAAAACTTTAAGTCGGCAGAAGATATGATTAAGTTGTATAGAGAACTTTGTACTGAGTACCCAATTGTATCAATTGAAGATCCGTTTGATAAGGAGGACTGGGAACACACCAAACGCTTTTCTAATCTTGCAATTTGTCAGGTAGTTGGAGATGACTTGTTAATGTCAAATTCACAACGTATGAAGAAAGCAATAGAGGAATCTACATGTGATGCTCTTCTTCTGAAGATTAACCAGATTGGGACAGTGACTGAGGCCATTGAAGTCGTTAAACTGGCAAAGGATGCCCACTGGGGAGTTGTGGCATCTCATAGATGTGGTGAAACTGAAGAGTCCTTTATAGCTGACTTCTCTGTTGGTCTCTCTACTGGTCAAATCAAAGCTGGTGCTCCTTGCAGAAGCGAGCGACTGGCCAAGTATAACCAGTTGCTTCGAATTGAGGAAGAGCTTGGGGACGATGCATTTTATGCCGGTGAAGAGTGGAGGGAATCACCCTGA

Protein Analysis

479

Amino Acids

52.43

Weight (kDa)

5.54

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 47 - 180 6.1e-50 Enolase, N-terminal domain
Enolase_C PF00113 189 - 476 4.2e-119 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 319 - 441 1.1e-08 Enolase C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29560
fragaria_vesca FvH4_3g37690
malus_domestica MD03G1078500.v1.1
prunus_persica Prupe.6G063500_v2.0.a1
pyrus_communis pycom03g06290 pycom11g07120
rosa_chinensis RchiOBHm_Chr5g0067961
rosa_laevigata RLG00000035947
rosa_roxburghii Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa Rorug05G0386800
rosa_samantha Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 596
AccI GTMKAC 1 cut(s) 729
AciI CCGC 3 cut(s) 66, 84, 674
AclWI GGATC 8 cut(s) 83, 157, 316, 380, 393, 700, 819, 1016
AcoI YGGCCR 1 cut(s) 1353
AcsI RAATTY 1 cut(s) 1111
AcuI CTGAAG 4 cut(s) 216, 387, 1187, 1290
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 4 cut(s) 530, 901, 994, 1001
AfiI CCNNNNNNNGG 1 cut(s) 72
AflIII ACRYGT 1 cut(s) 1148
AgsI TTSAA 6 cut(s) 194, 287, 398, 715, 1019, 1205
AjnI CCWGG 1 cut(s) 912
AjuI GAANNNNNNNTTGG 2 cut(s) 360, 392
AluBI AGCT 8 cut(s) 155, 236, 338, 500, 662, 1286, 1325, 1390
AluI AGCT 8 cut(s) 155, 236, 338, 500, 662, 1286, 1325, 1390
Alw21I GWGCWC 1 cut(s) 1333
Alw26I GTCTC 1 cut(s) 1307
AlwI GGATC 8 cut(s) 83, 157, 316, 380, 393, 700, 819, 1016
AlwNI CAGNNNCTG 1 cut(s) 1193
AoxI GGCC 3 cut(s) 75, 1197, 1353
ApeKI GCWGC 3 cut(s) 488, 506, 878
ApoI RAATTY 1 cut(s) 1111
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 2 cut(s) 7, 75
AsuHPI GGTGA 7 cut(s) 275, 461, 773, 918, 1274, 1425, 1427
AsuII TTCGAA 1 cut(s) 1375
AvaII GGWCC 1 cut(s) 7
BalI TGGCCA 1 cut(s) 1355
BamHI GGATCC 1 cut(s) 385
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 1333
BbvI GCAGC 3 cut(s) 475, 493, 865
BccI CCATC 3 cut(s) 290, 376, 761
BceAI ACGGC 2 cut(s) 41, 53
BciT130I CCWGG 1 cut(s) 914
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 1307
BfaI CTAG 2 cut(s) 270, 626
BglI GCCNNNNNGGC 2 cut(s) 74, 473
BisI GCNGC 5 cut(s) 66, 84, 489, 507, 879
BlsI GCNGC 5 cut(s) 67, 85, 490, 508, 880
Bme1390I CCNGG 1 cut(s) 914
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 7, 75
BmiI GGNNCC 2 cut(s) 387, 522
BmrFI CCNGG 1 cut(s) 914
BmrI ACTGGG 2 cut(s) 1051, 1243
BmsI GCATC 4 cut(s) 1144, 1216, 1256, 1390
BmuI ACTGGG 2 cut(s) 1051, 1243
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 775
Bpu14I TTCGAA 1 cut(s) 1375
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 1307
BsaWI WCCGGW 1 cut(s) 852
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse118I RCCGGY 1 cut(s) 1412
Bse1I ACTGG 8 cut(s) 259, 792, 1046, 1221, 1238, 1315, 1356, 1366
Bse3DI GCAATG 2 cut(s) 423, 537
BseBI CCWGG 1 cut(s) 914
BseGI GGATG 3 cut(s) 387, 755, 1231
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMI GCAATG 2 cut(s) 423, 537
BseMII CTCAG 4 cut(s) 147, 524, 987, 1185
BseNI ACTGG 8 cut(s) 259, 792, 1046, 1221, 1238, 1315, 1356, 1366
BseXI GCAGC 3 cut(s) 475, 493, 865
BseYI CCCAGC 2 cut(s) 500, 614
BshFI GGCC 3 cut(s) 77, 1199, 1355
BsiHKAI GWGCWC 1 cut(s) 1333
BsiSI CCGG 2 cut(s) 853, 1413
BslFI GGGAC 2 cut(s) 1198, 1409
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 1 cut(s) 1307
BsmFI GGGAC 2 cut(s) 1198, 1409
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 3 cut(s) 77, 1199, 1355
Bso31I GGTCTC 1 cut(s) 1307
Bsp119I TTCGAA 1 cut(s) 1375
Bsp1286I GDGCHC 1 cut(s) 1333
Bsp1407I TGTACA 1 cut(s) 528
BspACI CCGC 3 cut(s) 66, 84, 674
BspANI GGCC 3 cut(s) 77, 1199, 1355
BspCNI CTCAG 4 cut(s) 146, 523, 988, 1186
BspLI GGNNCC 2 cut(s) 387, 522
BspPI GGATC 8 cut(s) 83, 157, 316, 380, 393, 700, 819, 1016
BspQI GCTCTTC 2 cut(s) 1164, 1380
BspT104I TTCGAA 1 cut(s) 1375
BspTNI GGTCTC 1 cut(s) 1307
BsrDI GCAATG 2 cut(s) 423, 537
BsrFI RCCGGY 1 cut(s) 1412
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 8 cut(s) 259, 792, 1046, 1221, 1238, 1315, 1356, 1366
BssAI RCCGGY 1 cut(s) 1412
BssNI GRCGYC 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 3 cut(s) 190, 528, 1189
Bst6I CTCTTC 5 cut(s) 104, 1164, 1266, 1380, 1413
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 2 cut(s) 1226, 1409
BstAUI TGTACA 1 cut(s) 528
BstBI TTCGAA 1 cut(s) 1375
BstC8I GCNNGC 3 cut(s) 493, 612, 1346
BstDEI CTNAG 4 cut(s) 133, 510, 996, 1194
BstF5I GGATG 3 cut(s) 387, 755, 1231
BstHHI GCGC 1 cut(s) 743
BstMAI GTCTC 1 cut(s) 1307
BstNI CCWGG 1 cut(s) 914
BstNSI RCATGY 2 cut(s) 223, 1152
BstSCI CCNGG 1 cut(s) 912
BstV1I GCAGC 3 cut(s) 475, 493, 865
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 4 cut(s) 162, 385, 811, 1021
BstYI RGATCY 4 cut(s) 162, 385, 811, 1021
BsuRI GGCC 3 cut(s) 77, 1199, 1355
BtsCI GGATG 3 cut(s) 387, 755, 1231
BtsIMutI CAGTG 4 cut(s) 141, 524, 1194, 1231
Cac8I GCNNGC 3 cut(s) 493, 612, 1346
CaiI CAGNNNCTG 1 cut(s) 1193
CfoI GCGC 1 cut(s) 743
Cfr10I RCCGGY 1 cut(s) 1412
Cfr13I GGNCC 2 cut(s) 7, 75
CseI GACGC 1 cut(s) 62
Csp6I GTAC 4 cut(s) 529, 900, 993, 1000
CviAII CATG 4 cut(s) 220, 746, 895, 1149
CviQI GTAC 4 cut(s) 529, 900, 993, 1000
DdeI CTNAG 4 cut(s) 133, 510, 996, 1194
EaeI YGGCCR 1 cut(s) 1353
Eam1104I CTCTTC 5 cut(s) 104, 1164, 1266, 1380, 1413
EarI CTCTTC 5 cut(s) 104, 1164, 1266, 1380, 1413
Eco31I GGTCTC 1 cut(s) 1307
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 4 cut(s) 216, 387, 1187, 1290
EcoRII CCWGG 1 cut(s) 912
EcoT22I ATGCAT 1 cut(s) 1405
FaeI CATG 4 cut(s) 223, 749, 898, 1152
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 2 cut(s) 1198, 1409
FatI CATG 4 cut(s) 219, 745, 894, 1148
FauNDI CATATG 1 cut(s) 114
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 1 cut(s) 729
Fnu4HI GCNGC 5 cut(s) 66, 84, 489, 507, 879
FokI GGATG 3 cut(s) 394, 762, 1238
Fsp4HI GCNGC 5 cut(s) 66, 84, 489, 507, 879
FspBI CTAG 2 cut(s) 270, 626
GlaI GCGC 1 cut(s) 742
GluI GCNGC 5 cut(s) 66, 84, 489, 507, 879
GsaI CCCAGC 2 cut(s) 504, 618
GsuI CTGGAG 1 cut(s) 775
HaeIII GGCC 3 cut(s) 77, 1199, 1355
HapII CCGG 2 cut(s) 853, 1413
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 743
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 4 cut(s) 223, 749, 898, 1152
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HinfI GANTC 5 cut(s) 127, 646, 1142, 1274, 1430
HpaII CCGG 2 cut(s) 853, 1413
HphI GGTGA 7 cut(s) 275, 461, 773, 918, 1274, 1425, 1427
Hpy166II GTNNAC 2 cut(s) 452, 730
Hpy188I TCNGA 7 cut(s) 240, 247, 320, 367, 513, 697, 1167
Hpy188III TCNNGA 3 cut(s) 20, 552, 635
Hpy8I GTNNAC 2 cut(s) 452, 730
HpyAV CCTTC 5 cut(s) 115, 139, 439, 709, 797
HpyCH4III ACNGT 3 cut(s) 190, 528, 1189
HpyCH4IV ACGT 1 cut(s) 1120
HpyCH4V TGCA 5 cut(s) 495, 894, 1073, 1338, 1403
HpyF3I CTNAG 4 cut(s) 133, 510, 996, 1194
HpySE526I ACGT 1 cut(s) 1120
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 4 cut(s) 223, 749, 898, 1152
HspAI GCGC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 409
LguI GCTCTTC 2 cut(s) 1164, 1380
LmnI GCTCC 4 cut(s) 241, 659, 784, 1336
Lsp1109I GCAGC 3 cut(s) 475, 493, 865
LweI GCATC 4 cut(s) 1144, 1216, 1256, 1390
MaeI CTAG 2 cut(s) 270, 626
MaeII ACGT 1 cut(s) 1120
MaeIII GTNAC 1 cut(s) 1189
MfeI CAATTG 4 cut(s) 654, 867, 1005, 1014
MflI RGATCY 4 cut(s) 162, 385, 811, 1021
MhlI GDGCHC 1 cut(s) 1333
MlsI TGGCCA 1 cut(s) 1355
MluCI AATT 9 cut(s) 287, 639, 654, 867, 1005, 1014, 1074, 1111, 1377
MluNI TGGCCA 1 cut(s) 1355
MlyI GAGTC 1 cut(s) 1283
MmeI TCCRAC 1 cut(s) 1069
Mox20I TGGCCA 1 cut(s) 1355
Mph1103I ATGCAT 1 cut(s) 1405
MscI TGGCCA 1 cut(s) 1355
MseI TTAA 6 cut(s) 342, 951, 972, 1103, 1173, 1212
MslI CAYNNNNRTG 2 cut(s) 748, 1257
Msp20I TGGCCA 1 cut(s) 1355
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 2 cut(s) 853, 1413
MspR9I CCNGG 1 cut(s) 914
MunI CAATTG 4 cut(s) 654, 867, 1005, 1014
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 1 cut(s) 914
NdeI CATATG 1 cut(s) 114
NlaIII CATG 4 cut(s) 223, 749, 898, 1152
NlaIV GGNNCC 2 cut(s) 387, 522
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 1189
NsiI ATGCAT 1 cut(s) 1405
NspI RCATGY 2 cut(s) 223, 1152
NspV TTCGAA 1 cut(s) 1375
PciI ACATGT 1 cut(s) 1148
PciSI GCTCTTC 2 cut(s) 1164, 1380
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 180
PfeI GAWTC 4 cut(s) 127, 646, 1142, 1430
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 5 cut(s) 67, 85, 490, 508, 880
PleI GAGTC 1 cut(s) 1282
PpsI GAGTC 1 cut(s) 1282
PscI ACATGT 1 cut(s) 1148
PsiI TTATAA 1 cut(s) 596
Psp6I CCWGG 1 cut(s) 912
PspFI CCCAGC 2 cut(s) 500, 614
PspGI CCWGG 1 cut(s) 912
PspN4I GGNNCC 2 cut(s) 387, 522
PspPI GGNCC 2 cut(s) 7, 75
PstNI CAGNNNCTG 1 cut(s) 1193
PsuI RGATCY 4 cut(s) 162, 385, 811, 1021
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 4 cut(s) 530, 901, 994, 1001
RsaNI GTAC 4 cut(s) 529, 900, 993, 1000
RseI CAYNNNNRTG 2 cut(s) 748, 1257
SapI GCTCTTC 2 cut(s) 1164, 1380
SaqAI TTAA 6 cut(s) 342, 951, 972, 1103, 1173, 1212
SatI GCNGC 5 cut(s) 66, 84, 489, 507, 879
Sau96I GGNCC 2 cut(s) 7, 75
SchI GAGTC 1 cut(s) 1283
ScrFI CCNGG 1 cut(s) 914
SduI GDGCHC 1 cut(s) 1333
SfaNI GCATC 4 cut(s) 1144, 1216, 1256, 1390
SfuI TTCGAA 1 cut(s) 1375
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 2 cut(s) 748, 1257
Sse9I AATT 9 cut(s) 287, 639, 654, 867, 1005, 1014, 1074, 1111, 1377
SsiI CCGC 3 cut(s) 66, 84, 674
SspMI CTAG 2 cut(s) 270, 626
StyD4I CCNGG 1 cut(s) 912
TaaI ACNGT 3 cut(s) 190, 528, 1189
TaiI ACGT 1 cut(s) 1123
TaqI TCGA 3 cut(s) 48, 168, 1375
TasI AATT 9 cut(s) 287, 639, 654, 867, 1005, 1014, 1074, 1111, 1377
TatI WGTACW 2 cut(s) 528, 992
TauI GCSGC 2 cut(s) 68, 86
TfiI GAWTC 4 cut(s) 127, 646, 1142, 1430
Tru1I TTAA 6 cut(s) 342, 951, 972, 1103, 1173, 1212
Tru9I TTAA 6 cut(s) 342, 951, 972, 1103, 1173, 1212
TscAI CASTG 4 cut(s) 141, 531, 1194, 1238
TseFI GTSAC 1 cut(s) 1189
TseI GCWGC 3 cut(s) 488, 506, 878
Tsp45I GTSAC 1 cut(s) 1189
TspDTI ATGAA 3 cut(s) 91, 194, 1139
TspGWI ACGGA 1 cut(s) 1014
TspRI CASTG 4 cut(s) 141, 531, 1194, 1238
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 1 cut(s) 1111
XceI RCATGY 2 cut(s) 223, 1152
XmiI GTMKAC 1 cut(s) 729
XspI CTAG 2 cut(s) 270, 626
Zsp2I ATGCAT 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.