Rw5G041620

Cytosolic enolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014315
Physical Location & Seq
Forward (+)
73225078 .. 73229689
4612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G041620.1

Sequence Viewer

Length: 1428 bp
ATGTCGGTCCAAGAGTATCTGGATAATCACTTGCTCTCTCGCAAAATCGAAGACGCCGTCAATGCCGCCGTCAGGGCCAAAGCCGCCGATCCCGTCCTCTTCATCTCGAATCATATGAGGAAGGCGATTCCCTCAGTGATAACGAAGGTCAAAGCTCGGCAGATCCTCGATAGCAGAGGCATTCCTACTGTTGAAGTTGATCTTCATACTAACAAGGGCATGTTTCGTGCTTCAGCTCCGAGTTCCGATCTTACTGGATTGTATGAGGCCGTTGAGTTGCGAGATGGGGATAAGGGATCGTATCTCGGAAGTAGTGTGAATAAAGCTGTTAAGAATGTGAATGAGAAAATATCTGAAGCATTGGTTGGGATGGATCCTACGCTTCAATCGCAGATTGATCAGGCAATGATAGACTTGGATAAGACAGAAAAGAAGGGTGAACTTGGAGTAAATGCCATATTGGCTGTGTCAATGGCTGCTTGCAAAGCTGGGGCTGCTCAGAAAGAGGTTCCACTGTACAAACACATTGCTGACCTTTCTGGAAGAAACACCCTGACCTTGCCCGTTCCTGCTTTCACTCTTATAAGTGGAGGAAAGCGTGCTGGGAATAGTCTAGCCATTCAGGAAATTATGATTCTCCCAATTGGAGCTAACAGGTTTGCGGAGGCACTACAAATGGGATCAGAAACTTATCATCACTTGAAGGCTGTTATTGTAGACAAGTATGGCGCACATGGATGTAATGTCGGTGAAGATGGTGGTTTTGCTCCCAACATCTCCAGTTTTAGAGAAGGCTTGGATCTTGTAAAAGAGGCTATCAGCAGAACAGGCTATAATGACCGGATAAAAATAGCAATTGATGTTGCTGCTACGAACTTCTGCATGGGTACGAAGTATGACCTGGACTACAAATCACCGAATAAGTCAGGGCAAAACTTTAAGTCGGCAGAAGATATGATTAAGTTGTATAGAGAACTTTGTACTGAGTACCCAATTGTATCAATTGAAGATCCGTTTGATAAGGAGGACTGGGAACACACCAAACGCTTTTCTAATCTTGCAATTTGTCAGGTAGTTGGAGATGACTTGTTAATGTCAAATTCACAACGTATGAAGAAAGCAATAGAGGAATCTACGTGTGATGCTCTTCTTCTGAAGATTAACCAGATTGGGACAGTGACTGAGGCCATTGAAGTCGTTAAACTGGCAAAGGATGCCCACTGGGGAGTTGTGGCATCTCATAGATGTGGTGAAACTGAGGAGTCCTTTATAGCTGACTTCTCTGTTGGTCTCTCTACTGGTCAAATCAAAGCTGGTGCTCCTTGCAGAAGCGAGCGACTGGCCAAGTATAACCAGTTGCTTCGAATTGAGGAAGAGCTTGGGGACGATGCATTTTATGCCGGTGAAGAGTGGAGGGAATCACCCTGA

Protein Analysis

475

Amino Acids

51.95

Weight (kDa)

5.54

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 47 - 176 2e-52 Enolase, N-terminal domain
Enolase_C PF00113 185 - 472 4.1e-119 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 315 - 437 1e-08 Enolase C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29560
fragaria_vesca FvH4_3g37690
malus_domestica MD03G1078500.v1.1
prunus_persica Prupe.6G063500_v2.0.a1
pyrus_communis pycom03g06290 pycom11g07120
rosa_chinensis RchiOBHm_Chr5g0067961
rosa_laevigata RLG00000035947
rosa_roxburghii Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa Rorug05G0386800
rosa_samantha Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
AccI GTMKAC 1 cut(s) 717
AciI CCGC 3 cut(s) 66, 84, 662
AclWI GGATC 8 cut(s) 83, 157, 304, 368, 381, 688, 807, 1004
AcoI YGGCCR 1 cut(s) 1341
AcsI RAATTY 1 cut(s) 1099
AcuI CTGAAG 3 cut(s) 216, 375, 1175
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 4 cut(s) 518, 889, 982, 989
AfiI CCNNNNNNNGG 1 cut(s) 72
AflIII ACRYGT 1 cut(s) 1136
AgsI TTSAA 5 cut(s) 194, 386, 703, 1007, 1193
AjnI CCWGG 1 cut(s) 900
AjuI GAANNNNNNNTTGG 2 cut(s) 348, 380
AluBI AGCT 8 cut(s) 155, 236, 326, 488, 650, 1274, 1313, 1378
AluI AGCT 8 cut(s) 155, 236, 326, 488, 650, 1274, 1313, 1378
Alw21I GWGCWC 1 cut(s) 1321
Alw26I GTCTC 1 cut(s) 1295
AlwI GGATC 8 cut(s) 83, 157, 304, 368, 381, 688, 807, 1004
AlwNI CAGNNNCTG 1 cut(s) 1181
AoxI GGCC 4 cut(s) 75, 267, 1185, 1341
ApeKI GCWGC 3 cut(s) 476, 494, 866
ApoI RAATTY 1 cut(s) 1099
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 2 cut(s) 7, 75
AsuHPI GGTGA 6 cut(s) 449, 761, 906, 1262, 1413, 1415
AsuII TTCGAA 1 cut(s) 1363
AvaII GGWCC 1 cut(s) 7
BalI TGGCCA 1 cut(s) 1343
BamHI GGATCC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 1321
BbvI GCAGC 3 cut(s) 463, 481, 853
BccI CCATC 3 cut(s) 278, 364, 749
BceAI ACGGC 3 cut(s) 41, 53, 254
BciT130I CCWGG 1 cut(s) 902
BclI TGATCA 1 cut(s) 397
BcoDI GTCTC 1 cut(s) 1295
BfaI CTAG 1 cut(s) 614
BglI GCCNNNNNGGC 2 cut(s) 74, 461
BisI GCNGC 5 cut(s) 66, 84, 477, 495, 867
BlsI GCNGC 5 cut(s) 67, 85, 478, 496, 868
Bme1390I CCNGG 1 cut(s) 902
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 7, 75
BmiI GGNNCC 2 cut(s) 375, 510
BmrFI CCNGG 1 cut(s) 902
BmrI ACTGGG 2 cut(s) 1039, 1231
BmsI GCATC 4 cut(s) 1132, 1204, 1244, 1378
BmuI ACTGGG 2 cut(s) 1039, 1231
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 763
Bpu14I TTCGAA 1 cut(s) 1363
BsaAI YACGTR 1 cut(s) 1137
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 1295
BsaWI WCCGGW 1 cut(s) 840
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse118I RCCGGY 1 cut(s) 1400
Bse1I ACTGG 8 cut(s) 259, 780, 1034, 1209, 1226, 1303, 1344, 1354
Bse3DI GCAATG 2 cut(s) 411, 525
BseBI CCWGG 1 cut(s) 902
BseGI GGATG 3 cut(s) 375, 743, 1219
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMI GCAATG 2 cut(s) 411, 525
BseMII CTCAG 5 cut(s) 147, 512, 975, 1173, 1248
BseNI ACTGG 8 cut(s) 259, 780, 1034, 1209, 1226, 1303, 1344, 1354
BseRI GAGGAG 1 cut(s) 1274
BseXI GCAGC 3 cut(s) 463, 481, 853
BseYI CCCAGC 2 cut(s) 488, 602
BshFI GGCC 4 cut(s) 77, 269, 1187, 1343
BsiHKAI GWGCWC 1 cut(s) 1321
BsiSI CCGG 2 cut(s) 841, 1401
BslFI GGGAC 2 cut(s) 1186, 1397
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 1 cut(s) 1295
BsmFI GGGAC 2 cut(s) 1186, 1397
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 4 cut(s) 77, 269, 1187, 1343
Bso31I GGTCTC 1 cut(s) 1295
Bsp119I TTCGAA 1 cut(s) 1363
Bsp1286I GDGCHC 1 cut(s) 1321
Bsp1407I TGTACA 1 cut(s) 516
BspACI CCGC 3 cut(s) 66, 84, 662
BspANI GGCC 4 cut(s) 77, 269, 1187, 1343
BspCNI CTCAG 5 cut(s) 146, 511, 976, 1174, 1249
BspLI GGNNCC 2 cut(s) 375, 510
BspPI GGATC 8 cut(s) 83, 157, 304, 368, 381, 688, 807, 1004
BspQI GCTCTTC 2 cut(s) 1152, 1368
BspT104I TTCGAA 1 cut(s) 1363
BspTNI GGTCTC 1 cut(s) 1295
BsrDI GCAATG 2 cut(s) 411, 525
BsrFI RCCGGY 1 cut(s) 1400
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 8 cut(s) 259, 780, 1034, 1209, 1226, 1303, 1344, 1354
BssAI RCCGGY 1 cut(s) 1400
BssNI GRCGYC 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 902
Bst4CI ACNGT 3 cut(s) 190, 516, 1177
Bst6I CTCTTC 4 cut(s) 104, 1152, 1368, 1401
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 2 cut(s) 1214, 1397
BstAUI TGTACA 1 cut(s) 516
BstBAI YACGTR 1 cut(s) 1137
BstBI TTCGAA 1 cut(s) 1363
BstC8I GCNNGC 3 cut(s) 481, 600, 1334
BstDEI CTNAG 5 cut(s) 133, 498, 984, 1182, 1257
BstF5I GGATG 3 cut(s) 375, 743, 1219
BstHHI GCGC 1 cut(s) 731
BstMAI GTCTC 1 cut(s) 1295
BstNI CCWGG 1 cut(s) 902
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 900
BstV1I GCAGC 3 cut(s) 463, 481, 853
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 4 cut(s) 162, 373, 799, 1009
BstYI RGATCY 4 cut(s) 162, 373, 799, 1009
BsuRI GGCC 4 cut(s) 77, 269, 1187, 1343
BtsCI GGATG 3 cut(s) 375, 743, 1219
BtsIMutI CAGTG 4 cut(s) 141, 512, 1182, 1219
Cac8I GCNNGC 3 cut(s) 481, 600, 1334
CaiI CAGNNNCTG 1 cut(s) 1181
CfoI GCGC 1 cut(s) 731
Cfr10I RCCGGY 1 cut(s) 1400
Cfr13I GGNCC 2 cut(s) 7, 75
CseI GACGC 1 cut(s) 62
Csp6I GTAC 4 cut(s) 517, 888, 981, 988
CviAII CATG 3 cut(s) 220, 734, 883
CviQI GTAC 4 cut(s) 517, 888, 981, 988
DdeI CTNAG 5 cut(s) 133, 498, 984, 1182, 1257
EaeI YGGCCR 1 cut(s) 1341
Eam1104I CTCTTC 4 cut(s) 104, 1152, 1368, 1401
EarI CTCTTC 4 cut(s) 104, 1152, 1368, 1401
Eco31I GGTCTC 1 cut(s) 1295
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 3 cut(s) 216, 375, 1175
EcoRII CCWGG 1 cut(s) 900
EcoT22I ATGCAT 1 cut(s) 1393
FaeI CATG 3 cut(s) 223, 737, 886
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 2 cut(s) 1186, 1397
FatI CATG 3 cut(s) 219, 733, 882
FauNDI CATATG 1 cut(s) 114
FbaI TGATCA 1 cut(s) 397
FblI GTMKAC 1 cut(s) 717
Fnu4HI GCNGC 5 cut(s) 66, 84, 477, 495, 867
FokI GGATG 3 cut(s) 382, 750, 1226
Fsp4HI GCNGC 5 cut(s) 66, 84, 477, 495, 867
FspBI CTAG 1 cut(s) 614
GlaI GCGC 1 cut(s) 730
GluI GCNGC 5 cut(s) 66, 84, 477, 495, 867
GsaI CCCAGC 2 cut(s) 492, 606
GsuI CTGGAG 1 cut(s) 763
HaeIII GGCC 4 cut(s) 77, 269, 1187, 1343
HapII CCGG 2 cut(s) 841, 1401
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 731
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 3 cut(s) 223, 737, 886
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HinfI GANTC 6 cut(s) 109, 127, 634, 1130, 1262, 1418
HpaII CCGG 2 cut(s) 841, 1401
HphI GGTGA 6 cut(s) 449, 761, 906, 1262, 1413, 1415
Hpy166II GTNNAC 2 cut(s) 440, 718
Hpy188I TCNGA 7 cut(s) 240, 247, 308, 355, 501, 685, 1155
Hpy188III TCNNGA 4 cut(s) 20, 106, 540, 623
Hpy8I GTNNAC 2 cut(s) 440, 718
HpyAV CCTTC 5 cut(s) 115, 139, 427, 697, 785
HpyCH4III ACNGT 3 cut(s) 190, 516, 1177
HpyCH4IV ACGT 2 cut(s) 1108, 1136
HpyCH4V TGCA 5 cut(s) 483, 882, 1061, 1326, 1391
HpyF3I CTNAG 5 cut(s) 133, 498, 984, 1182, 1257
HpySE526I ACGT 2 cut(s) 1108, 1136
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 3 cut(s) 223, 737, 886
HspAI GCGC 1 cut(s) 729
Ksp22I TGATCA 1 cut(s) 397
LguI GCTCTTC 2 cut(s) 1152, 1368
LmnI GCTCC 4 cut(s) 241, 647, 772, 1324
Lsp1109I GCAGC 3 cut(s) 463, 481, 853
LweI GCATC 4 cut(s) 1132, 1204, 1244, 1378
MaeI CTAG 1 cut(s) 614
MaeII ACGT 2 cut(s) 1108, 1136
MaeIII GTNAC 1 cut(s) 1177
MfeI CAATTG 4 cut(s) 642, 855, 993, 1002
MflI RGATCY 4 cut(s) 162, 373, 799, 1009
MhlI GDGCHC 1 cut(s) 1321
MlsI TGGCCA 1 cut(s) 1343
MluCI AATT 8 cut(s) 627, 642, 855, 993, 1002, 1062, 1099, 1365
MluNI TGGCCA 1 cut(s) 1343
MlyI GAGTC 1 cut(s) 1271
MmeI TCCRAC 1 cut(s) 1057
Mox20I TGGCCA 1 cut(s) 1343
Mph1103I ATGCAT 1 cut(s) 1393
MscI TGGCCA 1 cut(s) 1343
MseI TTAA 6 cut(s) 330, 939, 960, 1091, 1161, 1200
MslI CAYNNNNRTG 2 cut(s) 736, 1245
Msp20I TGGCCA 1 cut(s) 1343
MspI CCGG 2 cut(s) 841, 1401
MspR9I CCNGG 1 cut(s) 902
MunI CAATTG 4 cut(s) 642, 855, 993, 1002
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 1 cut(s) 902
NdeI CATATG 1 cut(s) 114
NlaIII CATG 3 cut(s) 223, 737, 886
NlaIV GGNNCC 2 cut(s) 375, 510
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 1177
NsiI ATGCAT 1 cut(s) 1393
NspI RCATGY 1 cut(s) 223
NspV TTCGAA 1 cut(s) 1363
PciSI GCTCTTC 2 cut(s) 1152, 1368
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 180
PfeI GAWTC 5 cut(s) 109, 127, 634, 1130, 1418
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 5 cut(s) 67, 85, 478, 496, 868
PleI GAGTC 1 cut(s) 1270
PpsI GAGTC 1 cut(s) 1270
Ppu21I YACGTR 1 cut(s) 1137
PsiI TTATAA 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 900
PspFI CCCAGC 2 cut(s) 488, 602
PspGI CCWGG 1 cut(s) 900
PspN4I GGNNCC 2 cut(s) 375, 510
PspPI GGNCC 2 cut(s) 7, 75
PstNI CAGNNNCTG 1 cut(s) 1181
PsuI RGATCY 4 cut(s) 162, 373, 799, 1009
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 4 cut(s) 518, 889, 982, 989
RsaNI GTAC 4 cut(s) 517, 888, 981, 988
RseI CAYNNNNRTG 2 cut(s) 736, 1245
SapI GCTCTTC 2 cut(s) 1152, 1368
SaqAI TTAA 6 cut(s) 330, 939, 960, 1091, 1161, 1200
SatI GCNGC 5 cut(s) 66, 84, 477, 495, 867
Sau96I GGNCC 2 cut(s) 7, 75
SchI GAGTC 1 cut(s) 1271
ScrFI CCNGG 1 cut(s) 902
SduI GDGCHC 1 cut(s) 1321
SfaNI GCATC 4 cut(s) 1132, 1204, 1244, 1378
SfuI TTCGAA 1 cut(s) 1363
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 2 cut(s) 736, 1245
Sse9I AATT 8 cut(s) 627, 642, 855, 993, 1002, 1062, 1099, 1365
SsiI CCGC 3 cut(s) 66, 84, 662
SspMI CTAG 1 cut(s) 614
StyD4I CCNGG 1 cut(s) 900
TaaI ACNGT 3 cut(s) 190, 516, 1177
TaiI ACGT 2 cut(s) 1111, 1139
TaqI TCGA 4 cut(s) 48, 107, 168, 1363
TasI AATT 8 cut(s) 627, 642, 855, 993, 1002, 1062, 1099, 1365
TatI WGTACW 2 cut(s) 516, 980
TauI GCSGC 2 cut(s) 68, 86
TfiI GAWTC 5 cut(s) 109, 127, 634, 1130, 1418
Tru1I TTAA 6 cut(s) 330, 939, 960, 1091, 1161, 1200
Tru9I TTAA 6 cut(s) 330, 939, 960, 1091, 1161, 1200
TscAI CASTG 4 cut(s) 141, 519, 1182, 1226
TseFI GTSAC 1 cut(s) 1177
TseI GCWGC 3 cut(s) 476, 494, 866
Tsp45I GTSAC 1 cut(s) 1177
TspDTI ATGAA 3 cut(s) 91, 194, 1127
TspGWI ACGGA 1 cut(s) 1002
TspRI CASTG 4 cut(s) 141, 519, 1182, 1226
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 1 cut(s) 1099
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 717
XspI CTAG 1 cut(s) 614
Zsp2I ATGCAT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.