pycom03g06290

Cytosolic enolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014315
Physical Location & Seq
Reverse (-)
4925380 .. 4930269
4890 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g06290.1

Sequence Viewer

Length: 1425 bp
ATGTCGGTGCAAGATTACTTGGATAACCACATGCTCTCTCGGAAAATCGAAGACGCCGTCAATGCCGCCGTTAGGGCTAAGGCCTTCGATCCCGTCCTCTTCATTTCGAATCATATGAAGAAAGCGGTTCCTTCAGTGATAACAAAGGTCAAAGCTCGGCAGATCCTCGATAGCAGAGGAATTCCAACCGTTGAAGTCGACCTCCACACTAACAAAGGAACGTTTCGTGCTTCAGCTCCTAGCTCTGATGTTTCCGGAATGTACGAGGCTGTTGAACTGCGAGATGGGGACAAGGGATTATATCTTGGAAATAGTGTGACTAAAGCTGTTAAGAATGTCAATGAGAAAATATCTGAAGCATTGGTAGGCATGGATCCTATGCTTCAGTCGCAGATTGATCAGGCAATGATAGACCTCGATAAAACAGAAAAGAAGAGTGAACTTGGAGTAAATGCCATATTGGCTGTGTCAATCGCTGCTTGCAAAGCTGGAGCTGCTCAAAAGGAGGTTCCACTGTACAAATATATTGCCGACCTTTCTGGAAAAGGCCACCTGACCCTGCCTGTTCCTGCTTTTACTGTCATTAGTGGAGGAAAGCATGCTGGGAATAATCTGGCCATTCAGGAAATTATGATTCTCCCAATTGGAGCTAGCAAATTTGAGGAGGCATTGCAAATGGGATCTGAAACCTATCATCACCTGAAGGCTGTTATTACAGAAAAATATGGCGCACATGGATGTAATGTTGGTGAAGATGGTGGTTTTGCTCCCAACATCTCGAGCGTTAGAGAAGTCTTGGATCTTGTAAAAGAGGCGATCAGTAGAACAGGTTATAATGAACGAATCAAACTAGCAATTGATTTTGCTGCTACTGACTTTTGCATAGGTACAAAGTATGACCTGGATTACAAATCAGTGGATAAGGGACAAAATTTCAAGTCGGCTGATGATATGATCGAGATGTATAAAGAACTTTGTAGTGAGTACCCAATTGTGTCAATTGAAGATCCGTTTGATAAAGAGGACTGGGAACACACTAAACGCTTTTCTAGTCTTGGAATTTGTCAGGTAGTTGGAGATGACTTGATAACGTCAAGTCTAAAGCGTATTAAGAGAGCTATAGAGGAGTCCACCTGCAATGCTCTACTCGTAAAGATAAACCAGATTGGAACAGTGACCGAGGCCATTGAAGTGATGAAGCTGGCGAAGGATGCCCACTGGGGAGTGGTCATGTCTCATAGATGTGGTGAAACTGAAGATTCCTTCATTGCCGACTTAGCTGTTGGTCTCTCAACTGGTCAGATCAAAGCTGGTGCTCCTTGCAGAGGCGAACGGCTGGCCAAGTATAACCAGTTACTTCGAATTGAGGAAGAACTTGGGGACGATGCATTTTATGCTGGTGAAGATTGGAGGCAACCATCCTGA

Protein Analysis

475

Amino Acids

51.84

Weight (kDa)

5.38

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 47 - 176 1.6e-52 Enolase, N-terminal domain
Enolase_C PF00113 185 - 472 5.8e-121 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 307 - 437 9.2e-10 Enolase C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29560
fragaria_vesca FvH4_3g37690
malus_domestica MD03G1078500.v1.1
prunus_persica Prupe.6G063500_v2.0.a1
pyrus_communis pycom03g06290 pycom11g07120
rosa_chinensis RchiOBHm_Chr5g0067961
rosa_laevigata RLG00000035947
rosa_roxburghii Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa Rorug05G0386800
rosa_samantha Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
AarI CACCTGC 1 cut(s) 1142
Acc36I ACCTGC 1 cut(s) 1142
AccI GTMKAC 1 cut(s) 198
AccIII TCCGGA 1 cut(s) 254
AciI CCGC 2 cut(s) 66, 125
AclI AACGTT 1 cut(s) 221
AclWI GGATC 7 cut(s) 83, 157, 368, 381, 688, 807, 1001
AcoI YGGCCR 2 cut(s) 615, 1338
AcsI RAATTY 4 cut(s) 180, 656, 931, 1059
AcuI CTGAAG 6 cut(s) 117, 216, 368, 375, 722, 1275
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 4 cut(s) 263, 518, 889, 986
AfiI CCNNNNNNNGG 2 cut(s) 72, 1325
AgsI TTSAA 5 cut(s) 194, 275, 937, 1004, 1190
AjnI CCWGG 1 cut(s) 900
Alw21I GWGCWC 1 cut(s) 1318
Alw26I GTCTC 2 cut(s) 1239, 1292
AlwI GGATC 7 cut(s) 83, 157, 368, 381, 688, 807, 1001
Ama87I CYCGRG 1 cut(s) 778
Aor13HI TCCGGA 1 cut(s) 254
AoxI GGCC 5 cut(s) 81, 547, 615, 1182, 1338
ApeKI GCWGC 3 cut(s) 476, 494, 866
ApoI RAATTY 4 cut(s) 180, 656, 931, 1059
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AspLEI GCGC 1 cut(s) 731
AsuHPI GGTGA 4 cut(s) 689, 761, 1259, 1412
AsuII TTCGAA 2 cut(s) 107, 1360
AsuNHI GCTAGC 1 cut(s) 650
AvaI CYCGRG 1 cut(s) 778
BalI TGGCCA 2 cut(s) 617, 1340
BamHI GGATCC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 1318
BbvI GCAGC 3 cut(s) 463, 481, 853
BccI CCATC 2 cut(s) 278, 749
BceAI ACGGC 3 cut(s) 41, 53, 1349
BciT130I CCWGG 1 cut(s) 902
BclI TGATCA 1 cut(s) 397
BcoDI GTCTC 2 cut(s) 1239, 1292
BfaI CTAG 4 cut(s) 240, 651, 851, 1050
BfmI CTRYAG 1 cut(s) 1119
BfuAI ACCTGC 1 cut(s) 1142
BglI GCCNNNNNGGC 2 cut(s) 74, 461
BisI GCNGC 4 cut(s) 66, 477, 495, 867
BlsI GCNGC 4 cut(s) 67, 478, 496, 868
Bme1390I CCNGG 1 cut(s) 902
BmeT110I CYCGRG 1 cut(s) 778
BmiI GGNNCC 3 cut(s) 129, 375, 510
BmrFI CCNGG 1 cut(s) 902
BmrI ACTGGG 2 cut(s) 1036, 1228
BmsI GCATC 2 cut(s) 1201, 1375
BmtI GCTAGC 1 cut(s) 654
BmuI ACTGGG 2 cut(s) 1036, 1228
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 2 cut(s) 107, 1360
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 1292
BsaJI CCNNGG 1 cut(s) 1179
BsaWI WCCGGW 1 cut(s) 254
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 1325
Bse1I ACTGG 4 cut(s) 1031, 1223, 1300, 1351
Bse3DI GCAATG 4 cut(s) 411, 668, 1144, 1266
BseAI TCCGGA 1 cut(s) 254
BseBI CCWGG 1 cut(s) 902
BseDI CCNNGG 1 cut(s) 1179
BseGI GGATG 3 cut(s) 743, 1216, 1418
BseLI CCNNNNNNNGG 2 cut(s) 72, 1325
BseMI GCAATG 4 cut(s) 411, 668, 1144, 1266
BseNI ACTGG 4 cut(s) 1031, 1223, 1300, 1351
BseRI GAGGAG 2 cut(s) 677, 1139
BseXI GCAGC 3 cut(s) 463, 481, 853
BseYI CCCAGC 1 cut(s) 602
BshFI GGCC 5 cut(s) 83, 549, 617, 1184, 1340
BsiHKAI GWGCWC 1 cut(s) 1318
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 3 cut(s) 302, 939, 1394
BslI CCNNNNNNNGG 2 cut(s) 72, 1325
BsmAI GTCTC 2 cut(s) 1239, 1292
BsmFI GGGAC 3 cut(s) 302, 939, 1394
BsnI GGCC 5 cut(s) 83, 549, 617, 1184, 1340
Bso31I GGTCTC 1 cut(s) 1292
BsoBI CYCGRG 1 cut(s) 778
Bsp119I TTCGAA 2 cut(s) 107, 1360
Bsp1286I GDGCHC 1 cut(s) 1318
Bsp13I TCCGGA 1 cut(s) 254
Bsp1407I TGTACA 1 cut(s) 516
BspACI CCGC 2 cut(s) 66, 125
BspANI GGCC 5 cut(s) 83, 549, 617, 1184, 1340
BspEI TCCGGA 1 cut(s) 254
BspLI GGNNCC 3 cut(s) 129, 375, 510
BspMI ACCTGC 1 cut(s) 1142
BspOI GCTAGC 1 cut(s) 654
BspPI GGATC 7 cut(s) 83, 157, 368, 381, 688, 807, 1001
BspT104I TTCGAA 2 cut(s) 107, 1360
BspTNI GGTCTC 1 cut(s) 1292
BsrDI GCAATG 4 cut(s) 411, 668, 1144, 1266
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 4 cut(s) 1031, 1223, 1300, 1351
BssECI CCNNGG 1 cut(s) 1179
BssNI GRCGYC 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 902
Bst4CI ACNGT 4 cut(s) 190, 516, 580, 1174
Bst6I CTCTTC 2 cut(s) 104, 428
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 1394
BstAUI TGTACA 1 cut(s) 516
BstBI TTCGAA 2 cut(s) 107, 1360
BstC8I GCNNGC 5 cut(s) 481, 600, 652, 1203, 1338
BstDEI CTNAG 2 cut(s) 78, 1276
BstENI CCTNNNNNAGG 1 cut(s) 1323
BstF5I GGATG 3 cut(s) 743, 1216, 1418
BstHHI GCGC 1 cut(s) 731
BstMAI GTCTC 2 cut(s) 1239, 1292
BstMWI GCNNNNNNNGC 9 cut(s) 62, 74, 388, 461, 485, 494, 1211, 1277, 1394
BstNI CCWGG 1 cut(s) 902
BstNSI RCATGY 2 cut(s) 34, 602
BstSCI CCNGG 1 cut(s) 900
BstSFI CTRYAG 1 cut(s) 1119
BstV1I GCAGC 3 cut(s) 463, 481, 853
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 5 cut(s) 162, 373, 680, 799, 1006
BstYI RGATCY 5 cut(s) 162, 373, 680, 799, 1006
BsuRI GGCC 5 cut(s) 83, 549, 617, 1184, 1340
BtsCI GGATG 3 cut(s) 743, 1216, 1418
BtsIMutI CAGTG 5 cut(s) 141, 512, 921, 1179, 1216
BveI ACCTGC 1 cut(s) 1142
Cac8I GCNNGC 5 cut(s) 481, 600, 652, 1203, 1338
CfoI GCGC 1 cut(s) 731
CseI GACGC 1 cut(s) 62
Csp6I GTAC 4 cut(s) 262, 517, 888, 985
CviAII CATG 5 cut(s) 31, 370, 599, 734, 1231
CviQI GTAC 4 cut(s) 262, 517, 888, 985
DdeI CTNAG 2 cut(s) 78, 1276
EaeI YGGCCR 2 cut(s) 615, 1338
Eam1104I CTCTTC 2 cut(s) 104, 428
EarI CTCTTC 2 cut(s) 104, 428
Eco147I AGGCCT 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 1292
Eco57I CTGAAG 6 cut(s) 117, 216, 368, 375, 722, 1275
Eco88I CYCGRG 1 cut(s) 778
EcoNI CCTNNNNNAGG 1 cut(s) 1323
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 900
EcoT22I ATGCAT 1 cut(s) 1390
FaeI CATG 5 cut(s) 34, 373, 602, 737, 1234
FaqI GGGAC 3 cut(s) 302, 939, 1394
FatI CATG 5 cut(s) 30, 369, 598, 733, 1230
FauNDI CATATG 1 cut(s) 114
FbaI TGATCA 1 cut(s) 397
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 4 cut(s) 66, 477, 495, 867
FokI GGATG 3 cut(s) 750, 1223, 1405
Fsp4HI GCNGC 4 cut(s) 66, 477, 495, 867
FspBI CTAG 4 cut(s) 240, 651, 851, 1050
GlaI GCGC 1 cut(s) 730
GluI GCNGC 4 cut(s) 66, 477, 495, 867
GsaI CCCAGC 1 cut(s) 606
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 5 cut(s) 83, 549, 617, 1184, 1340
HapII CCGG 1 cut(s) 255
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 731
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 5 cut(s) 34, 373, 602, 737, 1234
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 5 cut(s) 109, 634, 843, 1127, 1259
HpaII CCGG 1 cut(s) 255
HphI GGTGA 4 cut(s) 689, 761, 1259, 1412
Hpy166II GTNNAC 3 cut(s) 199, 440, 1131
Hpy188I TCNGA 5 cut(s) 42, 247, 355, 685, 1302
Hpy188III TCNNGA 6 cut(s) 255, 540, 623, 778, 958, 1422
Hpy8I GTNNAC 3 cut(s) 199, 440, 1131
HpyAV CCTTC 5 cut(s) 94, 141, 697, 1201, 1273
HpyCH4III ACNGT 4 cut(s) 190, 516, 580, 1174
HpyCH4IV ACGT 2 cut(s) 221, 1091
HpyCH4V TGCA 7 cut(s) 10, 483, 673, 882, 1137, 1323, 1388
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 74, 388, 461, 485, 494, 1211, 1277, 1394
HpyF3I CTNAG 2 cut(s) 78, 1276
HpySE526I ACGT 2 cut(s) 221, 1091
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 5 cut(s) 34, 373, 602, 737, 1234
HspAI GCGC 1 cut(s) 729
Kpn2I TCCGGA 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 397
LmnI GCTCC 5 cut(s) 241, 491, 647, 772, 1321
Lsp1109I GCAGC 3 cut(s) 463, 481, 853
LweI GCATC 2 cut(s) 1201, 1375
MaeI CTAG 4 cut(s) 240, 651, 851, 1050
MaeII ACGT 2 cut(s) 221, 1091
MaeIII GTNAC 3 cut(s) 316, 1174, 1353
MboII GAAGA 9 cut(s) 62, 91, 130, 445, 764, 1016, 1268, 1382, 1415
MfeI CAATTG 4 cut(s) 642, 855, 990, 999
MflI RGATCY 5 cut(s) 162, 373, 680, 799, 1006
MhlI GDGCHC 1 cut(s) 1318
MlsI TGGCCA 2 cut(s) 617, 1340
MluNI TGGCCA 2 cut(s) 617, 1340
MlyI GAGTC 1 cut(s) 1136
MmeI TCCRAC 2 cut(s) 209, 1054
Mox20I TGGCCA 2 cut(s) 617, 1340
Mph1103I ATGCAT 1 cut(s) 1390
MroI TCCGGA 1 cut(s) 254
MscI TGGCCA 2 cut(s) 617, 1340
MseI TTAA 2 cut(s) 330, 1110
MslI CAYNNNNRTG 3 cut(s) 736, 1190, 1242
Msp20I TGGCCA 2 cut(s) 617, 1340
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 902
MunI CAATTG 4 cut(s) 642, 855, 990, 999
MvaI CCWGG 1 cut(s) 902
MwoI GCNNNNNNNGC 9 cut(s) 62, 74, 388, 461, 485, 494, 1211, 1277, 1394
NdeI CATATG 1 cut(s) 114
NheI GCTAGC 1 cut(s) 650
NlaIII CATG 5 cut(s) 34, 373, 602, 737, 1234
NlaIV GGNNCC 3 cut(s) 129, 375, 510
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 2 cut(s) 316, 1174
NsiI ATGCAT 1 cut(s) 1390
NspI RCATGY 2 cut(s) 34, 602
NspV TTCGAA 2 cut(s) 107, 1360
PaeI GCATGC 1 cut(s) 602
PaeR7I CTCGAG 1 cut(s) 778
PaqCI CACCTGC 1 cut(s) 1142
PceI AGGCCT 1 cut(s) 83
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 4 cut(s) 109, 634, 843, 1259
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 4 cut(s) 67, 478, 496, 868
PleI GAGTC 1 cut(s) 1135
PpsI GAGTC 1 cut(s) 1135
PsiI TTATAA 1 cut(s) 834
Psp1406I AACGTT 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 900
PspFI CCCAGC 1 cut(s) 602
PspGI CCWGG 1 cut(s) 900
PspN4I GGNNCC 3 cut(s) 129, 375, 510
PsuI RGATCY 5 cut(s) 162, 373, 680, 799, 1006
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 4 cut(s) 263, 518, 889, 986
RsaNI GTAC 4 cut(s) 262, 517, 888, 985
RseI CAYNNNNRTG 3 cut(s) 736, 1190, 1242
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 330, 1110
SatI GCNGC 4 cut(s) 66, 477, 495, 867
SchI GAGTC 1 cut(s) 1136
ScrFI CCNGG 1 cut(s) 902
SduI GDGCHC 1 cut(s) 1318
SfaNI GCATC 2 cut(s) 1201, 1375
SfcI CTRYAG 1 cut(s) 1119
Sfr274I CTCGAG 1 cut(s) 778
SfuI TTCGAA 2 cut(s) 107, 1360
SlaI CTCGAG 1 cut(s) 778
SmiMI CAYNNNNRTG 3 cut(s) 736, 1190, 1242
SmlI CTYRAG 1 cut(s) 778
SmoI CTYRAG 1 cut(s) 778
SphI GCATGC 1 cut(s) 602
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 2 cut(s) 66, 125
SspMI CTAG 4 cut(s) 240, 651, 851, 1050
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 1 cut(s) 900
TaaI ACNGT 4 cut(s) 190, 516, 580, 1174
TaiI ACGT 2 cut(s) 224, 1094
TaqI TCGA 9 cut(s) 48, 87, 107, 168, 198, 417, 779, 957, 1360
TaqII GACCGA 1 cut(s) 1193
TatI WGTACW 1 cut(s) 516
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 4 cut(s) 109, 634, 843, 1259
Tru1I TTAA 2 cut(s) 330, 1110
Tru9I TTAA 2 cut(s) 330, 1110
TscAI CASTG 5 cut(s) 141, 519, 921, 1179, 1223
TseFI GTSAC 2 cut(s) 316, 1174
TseI GCWGC 3 cut(s) 476, 494, 866
Tsp45I GTSAC 2 cut(s) 316, 1174
TspDTI ATGAA 5 cut(s) 91, 131, 852, 1211, 1255
TspGWI ACGGA 1 cut(s) 999
TspRI CASTG 5 cut(s) 141, 519, 921, 1179, 1223
Tth111I GACNNNGTC 1 cut(s) 56
XagI CCTNNNNNAGG 1 cut(s) 1323
XapI RAATTY 4 cut(s) 180, 656, 931, 1059
XceI RCATGY 2 cut(s) 34, 602
XhoI CTCGAG 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 4 cut(s) 240, 651, 851, 1050
Zsp2I ATGCAT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.