FvH4_3g37690

Cytosolic enolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014315
Physical Location & Seq
Forward (+)
32282465 .. 32287812
5348 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37690.t1

Sequence Viewer

Length: 1302 bp
ATGTCGGTCCAAGAGTACCTGGACAATCACATGCTCTCTCGCAAAATCGAAGACGCCGTCAATGCCGCCGTCAGGGCCAAGGCCCCCGATCCCGTCCTCTTCATCTCGAATCATATGAGGAAGGCGATTCCTTCAGTGATAACGAAGCTCAAAGCTCGGCAGATCCTCGATAGCAGAGGAATTCCTACTGTTGAAGTTGATCTTCATACTAACAAAGGAGTGTTCCGAGCTTCGGCTCCCAGTTCCGATCTTACTGGAGCTGGTGAAACTAGGTATGAGGCTGTTGAGTTGCGAGATGGGGACAAGAAGTTGTATCTTGGAAGTAGTGTGACTAAAGCTGTTAAGAATGTGAATGAGAAGATATCTGAAGCCTTGGTTGGGATGGATCCTACGCTTCAATCGCAGATTGATCAGGCAATGATAGACTTGGATAAGACAGAAAAGAAGGGTGAGCTTGGAGTAAATGCCATATTGGCTGTTTCGATGGCTGCTTGCAAAGCTGGGGCTGCTCAGAAAGAGGTTCCACTGTACAAACATATTGCTGACCTTTCTGGAAGAAACACCCTGACCCTGCCTGTTCCTGCTTTCACTGTTATAAGTGGAGGCAAGCGTGCTGGGAATAGTCTGGCCATTCAGGAAATTATGATCCTGCCAATTGGAGCTAACAGGTTTGAGGAGGCACTGCAAATGGGATCAGAAACGTATCATCACTTGAAGGCTGTTATTTTAGAGAAGTATGGCTCAACTGGTTGTAATGTCGGTGAAGACGGTGGTTTTGCACCCAACATCTCCAGCTTTACTGAAGGCTTGGATCTTGTAAAAGAGGCCATCGGCAGAACAGGTTATAATGACCAGATAAAAATAGCAATTGATGTTGCTGCTACAGACTTCTGCATGGGTACGAAGTATGACCTGGACTACAAATCACCAAATAAGTCAGGGCAAAACTTTAAGACTGCAGAAGATATGATTAAGTTGTATAGTGAACTTTGTACTGCGTACGCAATCGTATCTATTGAAGATCCATTTGATAAGGAGGACTGGGAACACACTAAACGCTTTTCTAACCTTGCAATTTGTCAGGTAGTGGGAGATGACTTGTTAATGTCAAATTCACAACGTATGAAGAAAGCAATAGAGGAATCTACGTGTGATGCTCTTCTTTTGAAGGTTGGTCAGATCAAAGCTGGTGCTCCTTGCAGAGGCGAGCGACTGGCTAAATATAACCAGTTGCTTCGAATTGAGGAAGAGCTTGGGGACAATGCATTTTATGCTGGTGAAGAGTGGAGGGAGTCACCCTGA

Protein Analysis

434

Amino Acids

47.33

Weight (kDa)

5.49

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 47 - 180 3.2e-50 Enolase, N-terminal domain
Enolase_C PF00113 189 - 394 5.8e-72 Enolase, C-terminal TIM barrel domain
Enolase_C PF00113 390 - 430 4e-15 Enolase, C-terminal TIM barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29560
fragaria_vesca FvH4_3g37690
malus_domestica MD03G1078500.v1.1
prunus_persica Prupe.6G063500_v2.0.a1
pyrus_communis pycom03g06290 pycom11g07120
rosa_chinensis RchiOBHm_Chr5g0067961
rosa_laevigata RLG00000035947
rosa_roxburghii Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa Rorug05G0386800
rosa_samantha Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 596, 846
AciI CCGC 1 cut(s) 66
AclWI GGATC 8 cut(s) 83, 157, 380, 393, 640, 700, 819, 1016
AcoI YGGCCR 1 cut(s) 627
AcsI RAATTY 2 cut(s) 180, 1111
AcuI CTGAAG 3 cut(s) 117, 387, 822
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 5 cut(s) 17, 530, 901, 994, 1001
AfiI CCNNNNNNNGG 4 cut(s) 72, 232, 378, 1202
AflIII ACRYGT 1 cut(s) 1148
AgsI TTSAA 5 cut(s) 194, 398, 715, 1019, 1168
AjnI CCWGG 2 cut(s) 18, 912
AjuI GAANNNNNNNTTGG 2 cut(s) 360, 392
Alw21I GWGCWC 1 cut(s) 1195
AlwI GGATC 8 cut(s) 83, 157, 380, 393, 640, 700, 819, 1016
AoxI GGCC 4 cut(s) 75, 81, 627, 825
ApeKI GCWGC 3 cut(s) 488, 506, 878
ApoI RAATTY 2 cut(s) 180, 1111
ArsI GACNNNNNNTTYG 4 cut(s) 42, 74, 758, 790
AspS9I GGNCC 3 cut(s) 7, 75, 82
AsuHPI GGTGA 6 cut(s) 275, 461, 773, 918, 1287, 1289
AsuII TTCGAA 1 cut(s) 1237
AvaII GGWCC 1 cut(s) 7
BalI TGGCCA 1 cut(s) 629
BamHI GGATCC 1 cut(s) 385
BbsI GAAGAC 2 cut(s) 57, 771
Bbv12I GWGCWC 1 cut(s) 1195
BbvI GCAGC 3 cut(s) 475, 493, 865
BccI CCATC 4 cut(s) 290, 376, 478, 836
BceAI ACGGC 2 cut(s) 41, 53
BciT130I CCWGG 2 cut(s) 20, 914
BclI TGATCA 1 cut(s) 409
BfaI CTAG 1 cut(s) 270
BfmI CTRYAG 2 cut(s) 882, 957
BglI GCCNNNNNGGC 2 cut(s) 74, 473
BisI GCNGC 4 cut(s) 66, 489, 507, 879
BlsI GCNGC 4 cut(s) 67, 490, 508, 880
Bme1390I CCNGG 2 cut(s) 20, 914
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 3 cut(s) 7, 75, 82
BmiI GGNNCC 4 cut(s) 84, 237, 387, 522
BmrFI CCNGG 2 cut(s) 20, 914
BmrI ACTGGG 2 cut(s) 234, 1051
BmsI GCATC 1 cut(s) 1144
BmuI ACTGGG 2 cut(s) 234, 1051
BpiI GAAGAC 2 cut(s) 57, 771
BpmI CTGGAG 2 cut(s) 276, 775
Bpu14I TTCGAA 1 cut(s) 1237
BsaAI YACGTR 1 cut(s) 1149
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 78, 372
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 232, 378, 1202
Bse1I ACTGG 6 cut(s) 240, 259, 751, 1046, 1218, 1228
Bse3DI GCAATG 1 cut(s) 423
BseBI CCWGG 2 cut(s) 20, 914
BseDI CCNNGG 2 cut(s) 78, 372
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 4 cut(s) 72, 232, 378, 1202
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 524
BseNI ACTGG 6 cut(s) 240, 259, 751, 1046, 1218, 1228
BseRI GAGGAG 1 cut(s) 689
BseXI GCAGC 3 cut(s) 475, 493, 865
BseYI CCCAGC 2 cut(s) 500, 614
BshFI GGCC 4 cut(s) 77, 83, 629, 827
BsiHKAI GWGCWC 1 cut(s) 1195
BsiWI CGTACG 1 cut(s) 999
BslFI GGGAC 2 cut(s) 314, 1271
BslI CCNNNNNNNGG 4 cut(s) 72, 232, 378, 1202
BsmFI GGGAC 2 cut(s) 314, 1271
BsnI GGCC 4 cut(s) 77, 83, 629, 827
Bsp119I TTCGAA 1 cut(s) 1237
Bsp1286I GDGCHC 1 cut(s) 1195
Bsp1407I TGTACA 1 cut(s) 528
BspACI CCGC 1 cut(s) 66
BspANI GGCC 4 cut(s) 77, 83, 629, 827
BspCNI CTCAG 1 cut(s) 523
BspLI GGNNCC 4 cut(s) 84, 237, 387, 522
BspMAI CTGCAG 1 cut(s) 961
BspPI GGATC 8 cut(s) 83, 157, 380, 393, 640, 700, 819, 1016
BspQI GCTCTTC 2 cut(s) 1164, 1242
BspT104I TTCGAA 1 cut(s) 1237
BsrDI GCAATG 1 cut(s) 423
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 6 cut(s) 240, 259, 751, 1046, 1218, 1228
BssECI CCNNGG 2 cut(s) 78, 372
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 2 cut(s) 78, 372
Bst2UI CCWGG 2 cut(s) 20, 914
Bst4CI ACNGT 4 cut(s) 190, 528, 592, 770
Bst6I CTCTTC 4 cut(s) 104, 1164, 1242, 1275
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 1271
BstAUI TGTACA 1 cut(s) 528
BstBAI YACGTR 1 cut(s) 1149
BstBI TTCGAA 1 cut(s) 1237
BstC8I GCNNGC 4 cut(s) 493, 608, 612, 1208
BstDEI CTNAG 1 cut(s) 510
BstENI CCTNNNNNAGG 1 cut(s) 1200
BstF5I GGATG 1 cut(s) 387
BstMWI GCNNNNNNNGC 7 cut(s) 62, 74, 400, 473, 497, 506, 1271
BstNI CCWGG 2 cut(s) 20, 914
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 18, 912
BstSFI CTRYAG 2 cut(s) 882, 957
BstV1I GCAGC 3 cut(s) 475, 493, 865
BstV2I GAAGAC 2 cut(s) 57, 771
BstX2I RGATCY 4 cut(s) 162, 385, 811, 1021
BstYI RGATCY 4 cut(s) 162, 385, 811, 1021
BsuRI GGCC 4 cut(s) 77, 83, 629, 827
BtsCI GGATG 1 cut(s) 387
BtsI GCAGTG 1 cut(s) 680
BtsIMutI CAGTG 4 cut(s) 141, 524, 588, 680
Cac8I GCNNGC 4 cut(s) 493, 608, 612, 1208
Cfr13I GGNCC 3 cut(s) 7, 75, 82
CseI GACGC 1 cut(s) 62
Csp6I GTAC 5 cut(s) 16, 529, 900, 993, 1000
CviAII CATG 2 cut(s) 31, 895
CviQI GTAC 5 cut(s) 16, 529, 900, 993, 1000
DdeI CTNAG 1 cut(s) 510
EaeI YGGCCR 1 cut(s) 627
Eam1104I CTCTTC 4 cut(s) 104, 1164, 1242, 1275
EarI CTCTTC 4 cut(s) 104, 1164, 1242, 1275
Eco130I CCWWGG 2 cut(s) 78, 372
Eco32I GATATC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 3 cut(s) 117, 387, 822
EcoNI CCTNNNNNAGG 1 cut(s) 1200
EcoO109I RGGNCCY 1 cut(s) 82
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 18, 912
EcoRV GATATC 1 cut(s) 363
EcoT14I CCWWGG 2 cut(s) 78, 372
EcoT22I ATGCAT 1 cut(s) 1267
ErhI CCWWGG 2 cut(s) 78, 372
FaeI CATG 2 cut(s) 34, 898
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 2 cut(s) 314, 1271
FatI CATG 2 cut(s) 30, 894
FauNDI CATATG 1 cut(s) 114
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 4 cut(s) 66, 489, 507, 879
FokI GGATG 1 cut(s) 394
Fsp4HI GCNGC 4 cut(s) 66, 489, 507, 879
FspBI CTAG 1 cut(s) 270
GluI GCNGC 4 cut(s) 66, 489, 507, 879
GsaI CCCAGC 2 cut(s) 504, 618
GsuI CTGGAG 2 cut(s) 276, 775
HaeIII GGCC 4 cut(s) 77, 83, 629, 827
HgaI GACGC 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 2 cut(s) 34, 898
HinfI GANTC 4 cut(s) 109, 127, 1142, 1292
HphI GGTGA 6 cut(s) 275, 461, 773, 918, 1287, 1289
Hpy166II GTNNAC 1 cut(s) 986
Hpy188I TCNGA 6 cut(s) 227, 247, 367, 513, 697, 1179
Hpy188III TCNNGA 3 cut(s) 106, 552, 635
Hpy8I GTNNAC 1 cut(s) 986
HpyAV CCTTC 6 cut(s) 115, 141, 439, 709, 797, 1162
HpyCH4III ACNGT 4 cut(s) 190, 528, 592, 770
HpyCH4IV ACGT 3 cut(s) 701, 1120, 1148
HpyCH4V TGCA 8 cut(s) 495, 685, 779, 894, 959, 1073, 1200, 1265
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 74, 400, 473, 497, 506, 1271
HpyF3I CTNAG 1 cut(s) 510
HpySE526I ACGT 3 cut(s) 701, 1120, 1148
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 2 cut(s) 34, 898
Ksp22I TGATCA 1 cut(s) 409
LguI GCTCTTC 2 cut(s) 1164, 1242
LmnI GCTCC 4 cut(s) 241, 257, 659, 1198
Lsp1109I GCAGC 3 cut(s) 475, 493, 865
LweI GCATC 1 cut(s) 1144
MaeI CTAG 1 cut(s) 270
MaeII ACGT 3 cut(s) 701, 1120, 1148
MaeIII GTNAC 2 cut(s) 328, 1293
MfeI CAATTG 2 cut(s) 654, 867
MflI RGATCY 4 cut(s) 162, 385, 811, 1021
MhlI GDGCHC 1 cut(s) 1195
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 7 cut(s) 180, 639, 654, 867, 1074, 1111, 1239
MluNI TGGCCA 1 cut(s) 629
MlyI GAGTC 1 cut(s) 1301
Mox20I TGGCCA 1 cut(s) 629
Mph1103I ATGCAT 1 cut(s) 1267
MscI TGGCCA 1 cut(s) 629
MseI TTAA 4 cut(s) 342, 951, 972, 1103
Msp20I TGGCCA 1 cut(s) 629
MspR9I CCNGG 2 cut(s) 20, 914
MunI CAATTG 2 cut(s) 654, 867
MvaI CCWGG 2 cut(s) 20, 914
MwoI GCNNNNNNNGC 7 cut(s) 62, 74, 400, 473, 497, 506, 1271
NdeI CATATG 1 cut(s) 114
NlaIII CATG 2 cut(s) 34, 898
NlaIV GGNNCC 4 cut(s) 84, 237, 387, 522
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 2 cut(s) 328, 1293
NsiI ATGCAT 1 cut(s) 1267
NspI RCATGY 1 cut(s) 34
NspV TTCGAA 1 cut(s) 1237
PciSI GCTCTTC 2 cut(s) 1164, 1242
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 3 cut(s) 109, 127, 1142
Pfl23II CGTACG 1 cut(s) 999
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 4 cut(s) 67, 490, 508, 880
PleI GAGTC 1 cut(s) 1300
PpsI GAGTC 1 cut(s) 1300
Ppu21I YACGTR 1 cut(s) 1149
PsiI TTATAA 2 cut(s) 596, 846
Psp6I CCWGG 2 cut(s) 18, 912
PspFI CCCAGC 2 cut(s) 500, 614
PspGI CCWGG 2 cut(s) 18, 912
PspLI CGTACG 1 cut(s) 999
PspN4I GGNNCC 4 cut(s) 84, 237, 387, 522
PspPI GGNCC 3 cut(s) 7, 75, 82
PstI CTGCAG 1 cut(s) 961
PsuI RGATCY 4 cut(s) 162, 385, 811, 1021
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 5 cut(s) 17, 530, 901, 994, 1001
RsaNI GTAC 5 cut(s) 16, 529, 900, 993, 1000
SapI GCTCTTC 2 cut(s) 1164, 1242
SaqAI TTAA 4 cut(s) 342, 951, 972, 1103
SatI GCNGC 4 cut(s) 66, 489, 507, 879
Sau96I GGNCC 3 cut(s) 7, 75, 82
SchI GAGTC 1 cut(s) 1301
ScrFI CCNGG 2 cut(s) 20, 914
SduI GDGCHC 1 cut(s) 1195
SfaNI GCATC 1 cut(s) 1144
SfcI CTRYAG 2 cut(s) 882, 957
SfuI TTCGAA 1 cut(s) 1237
SinI GGWCC 1 cut(s) 7
Sse9I AATT 7 cut(s) 180, 639, 654, 867, 1074, 1111, 1239
SsiI CCGC 1 cut(s) 66
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 2 cut(s) 18, 912
StyI CCWWGG 2 cut(s) 78, 372
TaaI ACNGT 4 cut(s) 190, 528, 592, 770
TaiI ACGT 3 cut(s) 704, 1123, 1151
TaqI TCGA 5 cut(s) 48, 107, 168, 482, 1237
TasI AATT 7 cut(s) 180, 639, 654, 867, 1074, 1111, 1239
TatI WGTACW 2 cut(s) 528, 992
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 3 cut(s) 109, 127, 1142
Tru1I TTAA 4 cut(s) 342, 951, 972, 1103
Tru9I TTAA 4 cut(s) 342, 951, 972, 1103
TscAI CASTG 4 cut(s) 141, 531, 595, 687
TseFI GTSAC 2 cut(s) 328, 1293
TseI GCWGC 3 cut(s) 488, 506, 878
Tsp45I GTSAC 2 cut(s) 328, 1293
TspDTI ATGAA 3 cut(s) 91, 194, 1139
TspRI CASTG 4 cut(s) 141, 531, 595, 687
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 7
XagI CCTNNNNNAGG 1 cut(s) 1200
XapI RAATTY 2 cut(s) 180, 1111
XceI RCATGY 1 cut(s) 34
XspI CTAG 1 cut(s) 270
Zsp2I ATGCAT 1 cut(s) 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.