Rw0G015360

Cytosolic enolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00696
OG0014315
Physical Location & Seq
Reverse (-)
149 .. 4738
4590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015360.1

Sequence Viewer

Length: 1371 bp
ATGTCGGTCCAAGAGTATCTGGATAATCACTTGCTCTCCCGCAAAATCGAAGACGCCGTCAATGCCGCCGTTAGGGCCAAAGCCGCCGATCCCGTCCTCTTCATCTCGAATCATATGAGGAAGGCGATTCCCTCAGTGATAACGAAGGTCAAAGCTCGGCAGATCCTCGATAGTAGAGGAATTCCTACTGTTGAAGTTGATCTTCATACTAACAAGGGCATGTTTCGTGCTTCAGCTCCGAGTTCCGATCTTACTGGATTGTATGAGGCCGTTGAGTTGCGAGATGGGGATAAGGGATCGTATCTCGGAAGTAGTGTGAATAAAGCTGTTAAGAATGTGAATGAGAAAATATCTGAAGCATTGGTTGGGATGGATCCTACGCTTCAATCGCAGATTGATCAGGCAATGATAGACTTGGATAAGACAGAAAAGAAGGGTGAACTTGGAGTAAATGCCATATTGGCTGTGTCAATGGCTGCTTGCAAAGCTGGGGCTGCTCAGAAAGAGGTTCCACTGTACAAACACATTGCTGACCTTTCTGGAAGAAACACCCTGACCTTGCCCGTTCCTGCTTTCACTCTTATAAGTGGAGGAAAGCGTGCTGGGAATAGTCTAGCCATTCAGGAAATTATGATTCTCCCAATTGGAGCTAACAGGTTTGCGGAGGCACTACAAATGGGATCAGAAACTTATCATCACTTGAAGGCTGTTATTGTAGACAAGTATGGCGCACATGGATGTAATGTCGGTGAAGATGGTGGTTTTGCTCCCAACATCTCCAGTTTTAGAGAAGGCTTGGATCTTGTAAAAGAGGCTATCAGCAGAACAGGTACGAAGTATGACCTGGACTACAAATCACCGAATAAGTCAGGGCAAAACTTTAAGTCGGCAGAAGATATGATTAAGTTGTATAGAGAACTTTGTACTGAGTACCCAATTGTATCAATTGAAGATCCGTTTGATAAGGAGGACTGGGAACACACCAAACGCTTTTCTAATCTTGCAATTTGTCAGGTAGTTGGAGATGACTTGTTAATGTCAAATTCACAACGTATGAAGAAAGCAATAGAGGAATCTACGTGTGATGCTCTTCTTCTGAAGATTAACCAGATTGGGACAGTGACTGAAGCCATTGAAGTCGTTAAACTGGCAAAGGATGCCCACTGGGGAGTTGTGGCATCTCATAGATGTGGTGAAACTGAAGAGTCCTTTATAGCTGACTTCTCTGTTGGTCTTTCTACTGGTCAAATCAAAGCTGGTGCTCCTTGCAGAAGCGAGCGACTGGCCAAGTATAACCAGTTGCTTCGAATTGAGGAAGAGCTTGGGGACGATGCATTTTATGCCGGTGAAGAGTGGAGGGAATCACCCTGA

Protein Analysis

456

Amino Acids

49.85

Weight (kDa)

5.53

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 47 - 176 1.8e-52 Enolase, N-terminal domain
Enolase_C PF00113 185 - 278 2.8e-32 Enolase, C-terminal TIM barrel domain
Enolase_C PF00113 278 - 453 5.4e-73 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 296 - 418 1.1e-08 Enolase C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29560
fragaria_vesca FvH4_3g37690
malus_domestica MD03G1078500.v1.1
prunus_persica Prupe.6G063500_v2.0.a1
pyrus_communis pycom03g06290 pycom11g07120
rosa_chinensis RchiOBHm_Chr5g0067961
rosa_laevigata RLG00000035947
rosa_roxburghii Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa Rorug05G0386800
rosa_samantha Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
AccI GTMKAC 1 cut(s) 717
AciI CCGC 4 cut(s) 40, 66, 84, 662
AclWI GGATC 8 cut(s) 83, 157, 304, 368, 381, 688, 807, 947
AcoI YGGCCR 1 cut(s) 1284
AcsI RAATTY 2 cut(s) 180, 1042
AcuI CTGAAG 5 cut(s) 216, 375, 1118, 1146, 1221
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 4 cut(s) 518, 832, 925, 932
AfiI CCNNNNNNNGG 1 cut(s) 72
AflIII ACRYGT 1 cut(s) 1079
AgsI TTSAA 5 cut(s) 194, 386, 703, 950, 1136
AjnI CCWGG 1 cut(s) 843
AjuI GAANNNNNNNTTGG 2 cut(s) 348, 380
AluBI AGCT 8 cut(s) 155, 236, 326, 488, 650, 1217, 1256, 1321
AluI AGCT 8 cut(s) 155, 236, 326, 488, 650, 1217, 1256, 1321
Alw21I GWGCWC 1 cut(s) 1264
AlwI GGATC 8 cut(s) 83, 157, 304, 368, 381, 688, 807, 947
AlwNI CAGNNNCTG 1 cut(s) 1124
AoxI GGCC 3 cut(s) 75, 267, 1284
ApeKI GCWGC 2 cut(s) 476, 494
ApoI RAATTY 2 cut(s) 180, 1042
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 2 cut(s) 7, 75
AsuHPI GGTGA 6 cut(s) 449, 761, 849, 1205, 1356, 1358
AsuII TTCGAA 1 cut(s) 1306
AvaII GGWCC 1 cut(s) 7
BalI TGGCCA 1 cut(s) 1286
BamHI GGATCC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 1264
BbvI GCAGC 2 cut(s) 463, 481
BccI CCATC 3 cut(s) 278, 364, 749
BceAI ACGGC 3 cut(s) 41, 53, 254
BciT130I CCWGG 1 cut(s) 845
BclI TGATCA 1 cut(s) 397
BfaI CTAG 1 cut(s) 614
BglI GCCNNNNNGGC 2 cut(s) 74, 461
BisI GCNGC 4 cut(s) 66, 84, 477, 495
BlsI GCNGC 4 cut(s) 67, 85, 478, 496
Bme1390I CCNGG 1 cut(s) 845
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 7, 75
BmiI GGNNCC 2 cut(s) 375, 510
BmrFI CCNGG 1 cut(s) 845
BmrI ACTGGG 2 cut(s) 982, 1174
BmsI GCATC 4 cut(s) 1075, 1147, 1187, 1321
BmuI ACTGGG 2 cut(s) 982, 1174
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 763
Bpu14I TTCGAA 1 cut(s) 1306
BsaAI YACGTR 1 cut(s) 1080
BsaHI GRCGYC 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse118I RCCGGY 1 cut(s) 1343
Bse1I ACTGG 8 cut(s) 259, 780, 977, 1152, 1169, 1246, 1287, 1297
Bse3DI GCAATG 2 cut(s) 411, 525
BseBI CCWGG 1 cut(s) 845
BseGI GGATG 3 cut(s) 375, 743, 1162
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMI GCAATG 2 cut(s) 411, 525
BseMII CTCAG 3 cut(s) 147, 512, 918
BseNI ACTGG 8 cut(s) 259, 780, 977, 1152, 1169, 1246, 1287, 1297
BseXI GCAGC 2 cut(s) 463, 481
BseYI CCCAGC 2 cut(s) 488, 602
BshFI GGCC 3 cut(s) 77, 269, 1286
BsiHKAI GWGCWC 1 cut(s) 1264
BsiSI CCGG 1 cut(s) 1344
BslFI GGGAC 2 cut(s) 1129, 1340
BslI CCNNNNNNNGG 1 cut(s) 72
BsmFI GGGAC 2 cut(s) 1129, 1340
BsnI GGCC 3 cut(s) 77, 269, 1286
Bsp119I TTCGAA 1 cut(s) 1306
Bsp1286I GDGCHC 1 cut(s) 1264
Bsp1407I TGTACA 1 cut(s) 516
BspACI CCGC 4 cut(s) 40, 66, 84, 662
BspANI GGCC 3 cut(s) 77, 269, 1286
BspCNI CTCAG 3 cut(s) 146, 511, 919
BspLI GGNNCC 2 cut(s) 375, 510
BspPI GGATC 8 cut(s) 83, 157, 304, 368, 381, 688, 807, 947
BspQI GCTCTTC 2 cut(s) 1095, 1311
BspT104I TTCGAA 1 cut(s) 1306
BsrDI GCAATG 2 cut(s) 411, 525
BsrFI RCCGGY 1 cut(s) 1343
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 8 cut(s) 259, 780, 977, 1152, 1169, 1246, 1287, 1297
BssAI RCCGGY 1 cut(s) 1343
BssNI GRCGYC 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 845
Bst4CI ACNGT 3 cut(s) 190, 516, 1120
Bst6I CTCTTC 5 cut(s) 104, 1095, 1197, 1311, 1344
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 2 cut(s) 1157, 1340
BstAUI TGTACA 1 cut(s) 516
BstBAI YACGTR 1 cut(s) 1080
BstBI TTCGAA 1 cut(s) 1306
BstC8I GCNNGC 3 cut(s) 481, 600, 1277
BstDEI CTNAG 3 cut(s) 133, 498, 927
BstF5I GGATG 3 cut(s) 375, 743, 1162
BstHHI GCGC 1 cut(s) 731
BstMWI GCNNNNNNNGC 9 cut(s) 62, 74, 83, 388, 461, 485, 494, 1157, 1340
BstNI CCWGG 1 cut(s) 845
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 843
BstV1I GCAGC 2 cut(s) 463, 481
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 4 cut(s) 162, 373, 799, 952
BstYI RGATCY 4 cut(s) 162, 373, 799, 952
BsuRI GGCC 3 cut(s) 77, 269, 1286
BtsCI GGATG 3 cut(s) 375, 743, 1162
BtsIMutI CAGTG 4 cut(s) 141, 512, 1125, 1162
Cac8I GCNNGC 3 cut(s) 481, 600, 1277
CaiI CAGNNNCTG 1 cut(s) 1124
CfoI GCGC 1 cut(s) 731
Cfr10I RCCGGY 1 cut(s) 1343
Cfr13I GGNCC 2 cut(s) 7, 75
CseI GACGC 1 cut(s) 62
Csp6I GTAC 4 cut(s) 517, 831, 924, 931
CviAII CATG 2 cut(s) 220, 734
CviQI GTAC 4 cut(s) 517, 831, 924, 931
DdeI CTNAG 3 cut(s) 133, 498, 927
EaeI YGGCCR 1 cut(s) 1284
Eam1104I CTCTTC 5 cut(s) 104, 1095, 1197, 1311, 1344
EarI CTCTTC 5 cut(s) 104, 1095, 1197, 1311, 1344
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 5 cut(s) 216, 375, 1118, 1146, 1221
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 843
EcoT22I ATGCAT 1 cut(s) 1336
FaeI CATG 2 cut(s) 223, 737
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 2 cut(s) 1129, 1340
FatI CATG 2 cut(s) 219, 733
FauI CCCGC 1 cut(s) 47
FauNDI CATATG 1 cut(s) 114
FbaI TGATCA 1 cut(s) 397
FblI GTMKAC 1 cut(s) 717
Fnu4HI GCNGC 4 cut(s) 66, 84, 477, 495
FokI GGATG 3 cut(s) 382, 750, 1169
Fsp4HI GCNGC 4 cut(s) 66, 84, 477, 495
FspBI CTAG 1 cut(s) 614
GlaI GCGC 1 cut(s) 730
GluI GCNGC 4 cut(s) 66, 84, 477, 495
GsaI CCCAGC 2 cut(s) 492, 606
GsuI CTGGAG 1 cut(s) 763
HaeIII GGCC 3 cut(s) 77, 269, 1286
HapII CCGG 1 cut(s) 1344
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 731
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 2 cut(s) 223, 737
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HinfI GANTC 6 cut(s) 109, 127, 634, 1073, 1205, 1361
HpaII CCGG 1 cut(s) 1344
HphI GGTGA 6 cut(s) 449, 761, 849, 1205, 1356, 1358
Hpy166II GTNNAC 2 cut(s) 440, 718
Hpy188I TCNGA 7 cut(s) 240, 247, 308, 355, 501, 685, 1098
Hpy188III TCNNGA 4 cut(s) 20, 106, 540, 623
Hpy8I GTNNAC 2 cut(s) 440, 718
HpyAV CCTTC 5 cut(s) 115, 139, 427, 697, 785
HpyCH4III ACNGT 3 cut(s) 190, 516, 1120
HpyCH4IV ACGT 2 cut(s) 1051, 1079
HpyCH4V TGCA 4 cut(s) 483, 1004, 1269, 1334
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 74, 83, 388, 461, 485, 494, 1157, 1340
HpyF3I CTNAG 3 cut(s) 133, 498, 927
HpySE526I ACGT 2 cut(s) 1051, 1079
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 2 cut(s) 223, 737
HspAI GCGC 1 cut(s) 729
Ksp22I TGATCA 1 cut(s) 397
LguI GCTCTTC 2 cut(s) 1095, 1311
LmnI GCTCC 4 cut(s) 241, 647, 772, 1267
Lsp1109I GCAGC 2 cut(s) 463, 481
LweI GCATC 4 cut(s) 1075, 1147, 1187, 1321
MaeI CTAG 1 cut(s) 614
MaeII ACGT 2 cut(s) 1051, 1079
MaeIII GTNAC 1 cut(s) 1120
MfeI CAATTG 3 cut(s) 642, 936, 945
MflI RGATCY 4 cut(s) 162, 373, 799, 952
MhlI GDGCHC 1 cut(s) 1264
MlsI TGGCCA 1 cut(s) 1286
MluCI AATT 8 cut(s) 180, 627, 642, 936, 945, 1005, 1042, 1308
MluNI TGGCCA 1 cut(s) 1286
MlyI GAGTC 1 cut(s) 1214
MmeI TCCRAC 1 cut(s) 1000
Mox20I TGGCCA 1 cut(s) 1286
Mph1103I ATGCAT 1 cut(s) 1336
MscI TGGCCA 1 cut(s) 1286
MseI TTAA 6 cut(s) 330, 882, 903, 1034, 1104, 1143
MslI CAYNNNNRTG 2 cut(s) 736, 1188
Msp20I TGGCCA 1 cut(s) 1286
MspI CCGG 1 cut(s) 1344
MspR9I CCNGG 1 cut(s) 845
MunI CAATTG 3 cut(s) 642, 936, 945
MvaI CCWGG 1 cut(s) 845
MwoI GCNNNNNNNGC 9 cut(s) 62, 74, 83, 388, 461, 485, 494, 1157, 1340
NdeI CATATG 1 cut(s) 114
NlaIII CATG 2 cut(s) 223, 737
NlaIV GGNNCC 2 cut(s) 375, 510
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 1120
NsiI ATGCAT 1 cut(s) 1336
NspI RCATGY 1 cut(s) 223
NspV TTCGAA 1 cut(s) 1306
PciSI GCTCTTC 2 cut(s) 1095, 1311
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 5 cut(s) 109, 127, 634, 1073, 1361
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 4 cut(s) 67, 85, 478, 496
PleI GAGTC 1 cut(s) 1213
PpsI GAGTC 1 cut(s) 1213
Ppu21I YACGTR 1 cut(s) 1080
PsiI TTATAA 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 843
PspFI CCCAGC 2 cut(s) 488, 602
PspGI CCWGG 1 cut(s) 843
PspN4I GGNNCC 2 cut(s) 375, 510
PspPI GGNCC 2 cut(s) 7, 75
PstNI CAGNNNCTG 1 cut(s) 1124
PsuI RGATCY 4 cut(s) 162, 373, 799, 952
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 4 cut(s) 518, 832, 925, 932
RsaNI GTAC 4 cut(s) 517, 831, 924, 931
RseI CAYNNNNRTG 2 cut(s) 736, 1188
SapI GCTCTTC 2 cut(s) 1095, 1311
SaqAI TTAA 6 cut(s) 330, 882, 903, 1034, 1104, 1143
SatI GCNGC 4 cut(s) 66, 84, 477, 495
Sau96I GGNCC 2 cut(s) 7, 75
SchI GAGTC 1 cut(s) 1214
ScrFI CCNGG 1 cut(s) 845
SduI GDGCHC 1 cut(s) 1264
SfaNI GCATC 4 cut(s) 1075, 1147, 1187, 1321
SfuI TTCGAA 1 cut(s) 1306
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 2 cut(s) 736, 1188
Sse9I AATT 8 cut(s) 180, 627, 642, 936, 945, 1005, 1042, 1308
SsiI CCGC 4 cut(s) 40, 66, 84, 662
SspMI CTAG 1 cut(s) 614
StyD4I CCNGG 1 cut(s) 843
TaaI ACNGT 3 cut(s) 190, 516, 1120
TaiI ACGT 2 cut(s) 1054, 1082
TaqI TCGA 4 cut(s) 48, 107, 168, 1306
TasI AATT 8 cut(s) 180, 627, 642, 936, 945, 1005, 1042, 1308
TatI WGTACW 2 cut(s) 516, 923
TauI GCSGC 2 cut(s) 68, 86
TfiI GAWTC 5 cut(s) 109, 127, 634, 1073, 1361
Tru1I TTAA 6 cut(s) 330, 882, 903, 1034, 1104, 1143
Tru9I TTAA 6 cut(s) 330, 882, 903, 1034, 1104, 1143
TscAI CASTG 4 cut(s) 141, 519, 1125, 1169
TseFI GTSAC 1 cut(s) 1120
TseI GCWGC 2 cut(s) 476, 494
Tsp45I GTSAC 1 cut(s) 1120
TspDTI ATGAA 3 cut(s) 91, 194, 1070
TspGWI ACGGA 1 cut(s) 945
TspRI CASTG 4 cut(s) 141, 519, 1125, 1169
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 2 cut(s) 180, 1042
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 717
XspI CTAG 1 cut(s) 614
Zsp2I ATGCAT 1 cut(s) 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.