AT2G31530

Belongs to the SecY SEC61-alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008295
Physical Location & Seq
Forward (+)
13427011 .. 13430632
3622 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31530.1

Sequence Viewer

Length: 1728 bp
ATGAATTCCTCTCAAGCTTGCTTCTTCCATTTTTCTCTTAGACCAATCTCGCTTTCGCATCCCTCCTATGCCTTCCTCTCGAAACGAGACCCATTTCTGTGTTCTCAACCCAGAAAATGCCTTACTACGAATTTGAATATGTCAAGAACGAGACAAGGGCATTCTATACAAATGAACAGAAGACATCTTCTTATGAAAGAAAGGAAGAGTTTTTCGATAAATTATTCCGATAAGTTCAGAGATGATTCTATGAGTAGCGAGGAGATGCATACAGATGCTTTGGATGTGGAGATTATTCCGCCAGATTCACAGGACATTCGTAATTCACAGAACTCTGCTGTCTCCAACACACTACAGGATGATAGGCCAAAGTCTTTTAGAAATAGGTTTCTTGATTTTGTTCGGATAAGTTCAGTACTCAATACTGCAGCAGAAAGATTCTTTAAGAGCGAGATAAGGAGGAGGTTGTTTGTAACGGCTGTTTTGCTTGTACTTAGCCGTGTTGGATATTTTATACCTTTACCTGGATTCGACAGAAGATTAATACCTCAAGATTACCTTAGCTTTGTCTCGGGTTCTGTTGAGGAACTGGGTGAGTTTGGAGCAGAGATCAAGCTTTCGCTCTTCCAGCTTGGGCTCAGTCCACAAATAATAGCATCCATAATTATGCAGGTGCTTTGTCACGTTCTTCCATCCTTGGTGAAACTGCGAAAGGAAGGCTTAGATGGTCACGAGAAGATCAAGAGTTATATATGGTGGCTATCGTTTTTCTTCGCTATAGTGGAAGCATTAGTGGTGGCTTACACTTCTCTTCAATATTCAGTTTTTGCAGCCACTGCCCAGGTCAAGCATGTGATGATGACATCATCTCTGCTAGTCTGTGGTGCAATGACAATGACGTGGCTCTGTGATACAATATCAGAATCTGGATTTGGTCATGGCTCATCTCTGATTATTTGTGTGGGAATATTGACGGGCTACACAGAGACTTTACACAAGATGCTCAATCAAATCTCAGGAAGTTTCTCGAACTGGTTGCCTTACTTGTTAGGCTTGCTGGGCATTTTTACCGTAGTAACTATGTTCGCTGTTGTTGTTACTGAGGGCTGCAGGAAGATTAAACTACAATATTATGGTTTTAAACTTGCTTCTGCTTCGAGAGAAGGTTCTCCAATAACAGAGGTGGAGCCGTACATACCTTTTAACATCAACCCGGCTGGAATGCAACCTGTTCTCACAACCACATATCTTTTGGCATTCCCCAGCATTCTTGCAAGTATTCTGGGTTCACCTTTCTTGCTAAACATGAAGGAGATATTAAACCCTGAAAGCACAGTTGGAGCACCACCTTGGGTCTATTACTCAATATATGCGTTTTTCGTCTTTCTCTTCAACATATTTGACATTGCCAACTTGCCAAAGGAAATAGCGGATTATTTAAACAAAATGGGGGCGAGAATACCGAACATAAAGCCTGGGAAAGCCACAATCGAGTACTTAACGAAGATACAAGCATCAACTAGGTTTTGGGGTGGCCTTCTGTTGAGCTTTTTGGCAACAGCTTCTACTGTACTTGACCACTATCTACGCAGCATCAATCAAGGCTTTTCTATCGGATTTACATCTGTTTTGATCATTGTTGGTTCCATTATTGAACTCAGAAGATCTTACCATGCCTACAATGTAATGCCGAGTTTAAGCAAAGCGTTAAAGAGATATGGCGTATAA

Protein Analysis

575

Amino Acids

64.71

Weight (kDa)

9.24

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecY PF00344 207 - 552 7.4e-71 SecY
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 661
Acc36I ACCTGC 1 cut(s) 661
AciI CCGC 2 cut(s) 299, 1430
AcsI RAATTY 2 cut(s) 4, 130
AfaI GTAC 5 cut(s) 417, 492, 1193, 1496, 1572
AfiI CCNNNNNNNGG 1 cut(s) 524
AgsI TTSAA 4 cut(s) 136, 815, 1393, 1655
AjiI CACGTC 1 cut(s) 900
AjnI CCWGG 3 cut(s) 523, 840, 1474
AjuI GAANNNNNNNTTGG 6 cut(s) 37, 69, 915, 947, 1320, 1352
AluBI AGCT 6 cut(s) 17, 564, 616, 631, 1548, 1562
AluI AGCT 6 cut(s) 17, 564, 616, 631, 1548, 1562
Alw21I GWGCWC 1 cut(s) 1345
Alw26I GTCTC 5 cut(s) 81, 145, 346, 574, 980
AlwNI CAGNNNCTG 2 cut(s) 836, 926
Ama87I CYCGRG 1 cut(s) 571
AoxI GGCC 2 cut(s) 365, 1534
ApeKI GCWGC 4 cut(s) 428, 830, 1107, 1590
ApoI RAATTY 2 cut(s) 4, 130
AseI ATTAAT 1 cut(s) 542
AsuC2I CCSGG 1 cut(s) 1214
AsuHPI GGTGA 3 cut(s) 605, 712, 1281
AvaI CYCGRG 1 cut(s) 571
BanII GRGCYC 1 cut(s) 639
BauI CACGAG 1 cut(s) 731
BbsI GAAGAC 1 cut(s) 187
Bbv12I GWGCWC 1 cut(s) 1345
BbvI GCAGC 4 cut(s) 440, 842, 1094, 1602
BccI CCATC 2 cut(s) 700, 719
BceAI ACGGC 3 cut(s) 483, 492, 1174
BcgI CGANNNNNNTGC 2 cut(s) 1018, 1052
BciT130I CCWGG 3 cut(s) 525, 842, 1476
BclI TGATCA 1 cut(s) 1632
BcnI CCSGG 1 cut(s) 1214
BcoDI GTCTC 5 cut(s) 81, 145, 346, 574, 980
BfaI CTAG 2 cut(s) 875, 1521
BfmI CTRYAG 4 cut(s) 353, 426, 777, 1108
BfuAI ACCTGC 1 cut(s) 661
BglII AGATCT 1 cut(s) 1664
BisI GCNGC 4 cut(s) 429, 831, 1108, 1591
BlsI GCNGC 4 cut(s) 430, 832, 1109, 1592
BmcAI AGTACT 2 cut(s) 417, 1496
Bme1390I CCNGG 4 cut(s) 525, 842, 1214, 1476
BmeT110I CYCGRG 1 cut(s) 571
BmgBI CACGTC 1 cut(s) 900
BmiI GGNNCC 2 cut(s) 1188, 1645
BmrFI CCNGG 4 cut(s) 525, 842, 1214, 1476
BmrI ACTGGG 1 cut(s) 599
BmsI GCATC 7 cut(s) 67, 255, 265, 665, 990, 1523, 1602
BmuI ACTGGG 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 1214
BsaBI GATNNNNATC 1 cut(s) 1621
BsaI GGTCTC 1 cut(s) 81
BsaJI CCNNGG 4 cut(s) 696, 840, 1349, 1475
Bsc4I CCNNNNNNNGG 1 cut(s) 524
Bse1I ACTGG 2 cut(s) 594, 1037
Bse3DI GCAATG 2 cut(s) 894, 1404
Bse8I GATNNNNATC 1 cut(s) 1621
BseBI CCWGG 3 cut(s) 525, 842, 1476
BseDI CCNNGG 4 cut(s) 696, 840, 1349, 1475
BseGI GGATG 5 cut(s) 58, 289, 364, 656, 692
BseJI GATNNNNATC 1 cut(s) 1621
BseLI CCNNNNNNNGG 1 cut(s) 524
BseMI GCAATG 2 cut(s) 894, 1404
BseMII CTCAG 4 cut(s) 652, 1029, 1092, 1672
BseNI ACTGG 2 cut(s) 594, 1037
BseRI GAGGAG 2 cut(s) 275, 475
BseXI GCAGC 4 cut(s) 440, 842, 1094, 1602
BseYI CCCAGC 2 cut(s) 1057, 1262
BshFI GGCC 2 cut(s) 367, 1536
BsiHKAI GWGCWC 1 cut(s) 1345
BsiHKCI CYCGRG 1 cut(s) 571
BsiSI CCGG 1 cut(s) 1214
BslI CCNNNNNNNGG 1 cut(s) 524
BsmAI GTCTC 5 cut(s) 81, 145, 346, 574, 980
BsmI GAATGC 4 cut(s) 160, 1227, 1256, 1266
BsnI GGCC 2 cut(s) 367, 1536
Bso31I GGTCTC 1 cut(s) 81
BsoBI CYCGRG 1 cut(s) 571
Bsp1286I GDGCHC 2 cut(s) 639, 1345
Bsp143I GATC 4 cut(s) 609, 738, 1632, 1664
BspACI CCGC 2 cut(s) 299, 1430
BspANI GGCC 2 cut(s) 367, 1536
BspCNI CTCAG 4 cut(s) 651, 1028, 1093, 1671
BspLI GGNNCC 2 cut(s) 1188, 1645
BspMAI CTGCAG 2 cut(s) 430, 1112
BspMI ACCTGC 1 cut(s) 661
BspQI GCTCTTC 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 81
BsrDI GCAATG 2 cut(s) 894, 1404
BsrI ACTGG 2 cut(s) 594, 1037
BssECI CCNNGG 4 cut(s) 696, 840, 1349, 1475
BssMI GATC 4 cut(s) 609, 738, 1632, 1664
BssSI CACGAG 1 cut(s) 731
BssT1I CCWWGG 2 cut(s) 696, 1349
Bst2BI CACGAG 1 cut(s) 731
Bst2UI CCWGG 3 cut(s) 525, 842, 1476
Bst4CI ACNGT 3 cut(s) 1072, 1336, 1570
Bst6I CTCTTC 4 cut(s) 200, 629, 816, 1394
BstAPI GCANNNNNTGC 1 cut(s) 836
BstC8I GCNNGC 2 cut(s) 19, 1055
BstDEI CTNAG 8 cut(s) 38, 494, 560, 638, 721, 1015, 1101, 1658
BstF5I GGATG 5 cut(s) 58, 289, 364, 656, 692
BstKTI GATC 4 cut(s) 612, 741, 1635, 1667
BstMAI GTCTC 5 cut(s) 81, 145, 346, 574, 980
BstMBI GATC 4 cut(s) 609, 738, 1632, 1664
BstMWI GCNNNNNNNGC 3 cut(s) 628, 836, 1059
BstNI CCWGG 3 cut(s) 525, 842, 1476
BstNSI RCATGY 1 cut(s) 854
BstSCI CCNGG 4 cut(s) 523, 840, 1212, 1474
BstSFI CTRYAG 4 cut(s) 353, 426, 777, 1108
BstV1I GCAGC 4 cut(s) 440, 842, 1094, 1602
BstV2I GAAGAC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 1664
BstYI RGATCY 1 cut(s) 1664
BsuRI GGCC 2 cut(s) 367, 1536
BtrI CACGTC 1 cut(s) 900
BtsCI GGATG 5 cut(s) 58, 289, 364, 656, 692
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 1 cut(s) 834
BveI ACCTGC 1 cut(s) 661
Cac8I GCNNGC 2 cut(s) 19, 1055
CaiI CAGNNNCTG 2 cut(s) 836, 926
Csp6I GTAC 5 cut(s) 416, 491, 1192, 1495, 1571
CviAII CATG 4 cut(s) 851, 938, 1306, 1673
CviQI GTAC 5 cut(s) 416, 491, 1192, 1495, 1571
DdeI CTNAG 8 cut(s) 38, 494, 560, 638, 721, 1015, 1101, 1658
DpnI GATC 4 cut(s) 611, 740, 1634, 1666
DpnII GATC 4 cut(s) 609, 738, 1632, 1664
DraI TTTAAA 2 cut(s) 1141, 1440
Eam1104I CTCTTC 4 cut(s) 200, 629, 816, 1394
EarI CTCTTC 4 cut(s) 200, 629, 816, 1394
EciI GGCGGA 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 696, 1349
Eco24I GRGCYC 1 cut(s) 639
Eco31I GGTCTC 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 571
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 3 cut(s) 523, 840, 1474
EcoT14I CCWWGG 2 cut(s) 696, 1349
EcoT22I ATGCAT 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 639
ErhI CCWWGG 2 cut(s) 696, 1349
FaeI CATG 4 cut(s) 854, 941, 1309, 1676
FalI AAGNNNNNCTT 4 cut(s) 543, 575, 704, 736
FatI CATG 4 cut(s) 850, 937, 1305, 1672
FbaI TGATCA 1 cut(s) 1632
Fnu4HI GCNGC 4 cut(s) 429, 831, 1108, 1591
FokI GGATG 5 cut(s) 45, 296, 371, 643, 679
FriOI GRGCYC 1 cut(s) 639
Fsp4HI GCNGC 4 cut(s) 429, 831, 1108, 1591
FspBI CTAG 2 cut(s) 875, 1521
GluI GCNGC 4 cut(s) 429, 831, 1108, 1591
GsaI CCCAGC 2 cut(s) 1061, 1266
HaeIII GGCC 2 cut(s) 367, 1536
HapII CCGG 1 cut(s) 1214
Hin1II CATG 4 cut(s) 854, 941, 1309, 1676
HindIII AAGCTT 2 cut(s) 15, 614
HinfI GANTC 5 cut(s) 245, 305, 438, 528, 923
HpaII CCGG 1 cut(s) 1214
HphI GGTGA 3 cut(s) 605, 712, 1281
Hpy166II GTNNAC 2 cut(s) 644, 1289
Hpy188I TCNGA 7 cut(s) 229, 239, 405, 922, 951, 1616, 1661
Hpy8I GTNNAC 2 cut(s) 644, 1289
HpyAV CCTTC 5 cut(s) 82, 710, 1157, 1303, 1547
HpyCH4III ACNGT 3 cut(s) 1072, 1336, 1570
HpyCH4IV ACGT 2 cut(s) 684, 899
HpyCH4V TGCA 8 cut(s) 268, 428, 670, 830, 887, 1110, 1225, 1274
HpyF10VI GCNNNNNNNGC 3 cut(s) 628, 836, 1059
HpyF3I CTNAG 8 cut(s) 38, 494, 560, 638, 721, 1015, 1101, 1658
HpySE526I ACGT 2 cut(s) 684, 899
Hsp92II CATG 4 cut(s) 854, 941, 1309, 1676
Ksp22I TGATCA 1 cut(s) 1632
Kzo9I GATC 4 cut(s) 609, 738, 1632, 1664
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 3 cut(s) 602, 1186, 1340
Lsp1109I GCAGC 4 cut(s) 440, 842, 1094, 1602
LweI GCATC 7 cut(s) 67, 255, 265, 665, 990, 1523, 1602
MaeI CTAG 2 cut(s) 875, 1521
MaeII ACGT 2 cut(s) 684, 899
MaeIII GTNAC 5 cut(s) 472, 680, 728, 1075, 1096
MalI GATC 4 cut(s) 611, 740, 1634, 1666
MboI GATC 4 cut(s) 609, 738, 1632, 1664
MflI RGATCY 1 cut(s) 1664
MhlI GDGCHC 2 cut(s) 639, 1345
MluCI AATT 5 cut(s) 4, 130, 220, 322, 663
MmeI TCCRAC 3 cut(s) 369, 484, 1318
Mph1103I ATGCAT 1 cut(s) 270
MslI CAYNNNNRTG 3 cut(s) 97, 273, 665
MspI CCGG 1 cut(s) 1214
MspR9I CCNGG 4 cut(s) 525, 842, 1214, 1476
Mva1269I GAATGC 4 cut(s) 160, 1227, 1256, 1266
MvaI CCWGG 3 cut(s) 525, 842, 1476
MwoI GCNNNNNNNGC 3 cut(s) 628, 836, 1059
NciI CCSGG 1 cut(s) 1214
NdeII GATC 4 cut(s) 609, 738, 1632, 1664
NlaIII CATG 4 cut(s) 854, 941, 1309, 1676
NlaIV GGNNCC 2 cut(s) 1188, 1645
NmeAIII GCCGAG 1 cut(s) 1716
NmuCI GTSAC 2 cut(s) 680, 728
NsiI ATGCAT 1 cut(s) 270
NspI RCATGY 1 cut(s) 854
PaqCI CACCTGC 1 cut(s) 661
PciSI GCTCTTC 1 cut(s) 629
PctI GAATGC 4 cut(s) 160, 1227, 1256, 1266
PfeI GAWTC 5 cut(s) 245, 305, 438, 528, 923
PkrI GCNGC 4 cut(s) 430, 832, 1109, 1592
PshBI ATTAAT 1 cut(s) 542
Psp6I CCWGG 3 cut(s) 523, 840, 1474
PspFI CCCAGC 2 cut(s) 1057, 1262
PspGI CCWGG 3 cut(s) 523, 840, 1474
PspN4I GGNNCC 2 cut(s) 1188, 1645
PsrI GAACNNNNNNTAC 2 cut(s) 1270, 1302
PstI CTGCAG 2 cut(s) 430, 1112
PstNI CAGNNNCTG 2 cut(s) 836, 926
PsuI RGATCY 1 cut(s) 1664
RsaI GTAC 5 cut(s) 417, 492, 1193, 1496, 1572
RsaNI GTAC 5 cut(s) 416, 491, 1192, 1495, 1571
RseI CAYNNNNRTG 3 cut(s) 97, 273, 665
SapI GCTCTTC 1 cut(s) 629
SatI GCNGC 4 cut(s) 429, 831, 1108, 1591
Sau3AI GATC 4 cut(s) 609, 738, 1632, 1664
ScaI AGTACT 2 cut(s) 417, 1496
ScrFI CCNGG 4 cut(s) 525, 842, 1214, 1476
SduI GDGCHC 2 cut(s) 639, 1345
SfaNI GCATC 7 cut(s) 67, 255, 265, 665, 990, 1523, 1602
SfcI CTRYAG 4 cut(s) 353, 426, 777, 1108
SmiMI CAYNNNNRTG 3 cut(s) 97, 273, 665
SmlI CTYRAG 2 cut(s) 12, 549
SmoI CTYRAG 2 cut(s) 12, 549
Sse9I AATT 5 cut(s) 4, 130, 220, 322, 663
SsiI CCGC 2 cut(s) 299, 1430
SspI AATATT 3 cut(s) 818, 969, 1130
SspMI CTAG 2 cut(s) 875, 1521
StyD4I CCNGG 4 cut(s) 523, 840, 1212, 1474
StyI CCWWGG 2 cut(s) 696, 1349
TaaI ACNGT 3 cut(s) 1072, 1336, 1570
TaiI ACGT 2 cut(s) 687, 902
TaqI TCGA 6 cut(s) 80, 215, 531, 1028, 1157, 1491
TasI AATT 5 cut(s) 4, 130, 220, 322, 663
TatI WGTACW 4 cut(s) 415, 490, 1494, 1570
TfiI GAWTC 5 cut(s) 245, 305, 438, 528, 923
TscAI CASTG 1 cut(s) 841
TseFI GTSAC 2 cut(s) 680, 728
TseI GCWGC 4 cut(s) 428, 830, 1107, 1590
Tsp45I GTSAC 2 cut(s) 680, 728
TspDTI ATGAA 4 cut(s) 17, 188, 209, 1322
TspRI CASTG 1 cut(s) 841
VspI ATTAAT 1 cut(s) 542
XapI RAATTY 2 cut(s) 4, 130
XceI RCATGY 1 cut(s) 854
XcmI CCANNNNNNNNNTGG 1 cut(s) 1249
XspI CTAG 2 cut(s) 875, 1521
ZrmI AGTACT 2 cut(s) 417, 1496
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.