Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0008295

Physical Location & Seq

Genomic Coordinates Forward (+)

19379776 .. 19385157

Gene Structure 5382 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g23670.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGGAAGCCACCCTTCTCAGCTCCCAACGCTTCCACCCCCAACTCTTCCCCGCCAAACTTCGAGGTTCAGATACTCAAATTTATCGTCTTCCCCTGGTTAGAGCCAAAACCAGCTGCCTCTCCCTTAAGACCAATCCTAACCAATTATGCAGGACAAATTCAATTTTTCTAAACAGGCCTTTTGTCTCCAAACTAAGCACAAGAGTGTCTGCTAAGTTTTCGGACCAATTCCGAAGTGATTATACAGATGTTGAGGCCACACCTGTTGAATCCTTAAATGTTGACGTTGCTCCTCCGGGGCCGAAGGAGAGGACAGATGTTGTTAGAGTGGATGAGAGTGAGGATTCCCGTGGCGTGGAGGCGAAACCTGTAATGTTTAGGAACCGGTATTTGGATTTAGTGAGGATAGGTTCGGTGATTAATAATGCTGCTGAGTCGTTTTTCAAGAGTGAGATTAGGAGGAGGTTGTGTGTGACCGCAGTATTGATTGTGATTAGTCGTGTTGGTTATTTTATTCCGCTGCCGGGGTTTGATAGAAGGTTGATACCTCAGGACTATCTTAGCTTTGTCTCGGGATCAGTTGATGAGCTCGGGGATTTTACAGCAGAGCTTAAAATGTCCTTGTTCCAGCTTGGAATCAGTCCTCAAATTATAGCATCGATTATTATGCAGGTACTCTCTTATGTCATCCCCTCCCTAGCGAAGTTGCGGAAAGAAGGCTTAGATGGTCAAGAGAAGATTAAGAGTTATATATGGTGGATGTCTCTTGGCTTTGCAATTGTGGAGGCTGTGATACTGGCTTGTTATTCACTTCCCTATTCAATCTATGTAGCTAGCCACAGGGTCAAGCATGTTATGCTGACAAGTATGTTATTAGTCTGCGGTGCGATGACAATTACGTGGATCTGTGATACCATCACAGAATCTGGATTTGGCCAAGGTTCATCTCTTATAATATGTGTGCAAATATTGATCGGCTACACAGAGACATTATACAATATGTTGACCCAGCTCTCAGGAAGTTCTGTTAGTTGGTGGCCGTACTTATTTGGACTATTGGGCGTTTTCACAATAGTCACTATGTGGGCTGTTGTAGTAACTGAAGGTTGTAGGAAGATAAAGCTTCAGTACTATGGTTTTAAGCTTGCTTCTGCTGCAAGGGATGACTCACCTATTACTGAAGTGGAGCCCTATATCCCTTTAACTATTAATCCAGCAGGAATGCAGCCTGTTCTCACCACTACTTATCTCTTGGCATTTCCCAGCATTCTAGCAAGTCTTCTTGGTTCATCTTTCTGGTTGCATGTTAAAGAGATATTGAACCCCGATACTTCTATTGGTGCAGCGCCTTGGGTGTACTACTCCATATATGCATTTTTCGTCTTTTTATTCAACATATTGGACATTGCCAACTTGCCCAAGGAAATTGCTGATTATTTGAATAAGATGGGTGCCAGGATACCGAATATAAAACCTGGGAAGGCTACGATTGAATACCTCACAAAGGTTCAGGCATCAACACGCTTTTGGGGTGGTGTATTATTAAGCATCTTAGCAACATCATCAAGCATACTTGATCACTATTTACGTAATATCAACGCGGGATTTTCAATCGGGTTGACATCAGTCTTAATCATAGTGGGTTCGATTATCGAACTCAGAAGATCATATCAAGCTTACAATGTAATGCCGAGTTTAAGTAAAGCCTTAAGCCGGTATGGTGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008104 GO:0008150 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009668 GO:0009987 GO:0010027 GO:0016043 GO:0031967 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051641 GO:0061024 GO:0070727 GO:0071840 GO:0072598

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SecY

Protein Analysis

576

Amino Acids

64.11

Weight (kDa)

9.03

Isoelectric Point (pI)

39.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecY	PF00344	207 - 468	8.7e-49	SecY

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008295)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31530
fragaria_vesca	FvH4_2g23670 FvH4_2g23670 FvH4_2g23670 FvH4_2g23670 FvH4_2g23670 FvH4_2g23670
malus_domestica	MD03G1266100.v1.1 MD11G1286500.v1.1
prunus_persica	Prupe.8G241800_v2.0.a1
pyrus_communis	pycom03g21260 pycom11g25400
rosa_chinensis	RchiOBHm_Chr6g0291131
rosa_laevigata	RLG00000012141
rosa_multiflora	Rmu_sc0004642.1_g000022 Rmu_sc0013275.1_g000001
rosa_roxburghii	Rroxscaffold_7G00176350
rosa_rugosa	Rorug06G0217900
rosa_samantha	Rh6AG328200 Rh6BG335700 Rh6CG341800 Rh6DG329100
rosa_wichuraiana	Rw6G028550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	953
Acc36I	ACCTGC	1 cut(s)	661
AccB1I	GGYRCC	1 cut(s)	1451
AccII	CGCG	1 cut(s)	1601
AciI	CCGC	6 cut(s)	51, 477, 518, 709, 882, 1601
AclWI	GGATC	2 cut(s)	583, 911
AcoI	YGGCCR	2 cut(s)	934, 1037
AcsI	RAATTY	2 cut(s)	78, 157
AcuI	CTGAAG	3 cut(s)	1109, 1122, 1200
AdeI	CACNNNGTG	1 cut(s)	1083
AfaI	GTAC	4 cut(s)	675, 1043, 1130, 1358
AfiI	CCNNNNNNNGG	5 cut(s)	355, 391, 524, 1504, 1713
AflII	CTTAAG	2 cut(s)	125, 1708
AgeI	ACCGGT	1 cut(s)	384
AgsI	TTSAA	9 cut(s)	162, 269, 445, 822, 1321, 1393, 1441, 1493, 1611
AjnI	CCWGG	3 cut(s)	93, 1454, 1474
AjuI	GAANNNNNNNTTGG	4 cut(s)	374, 406, 915, 947
AleI	CACNNNNGTG	1 cut(s)	203
Alw21I	GWGCWC	1 cut(s)	591
Alw26I	GTCTC	4 cut(s)	190, 574, 768, 980
AlwI	GGATC	2 cut(s)	583, 911
AlwNI	CAGNNNCTG	1 cut(s)	926
Ama87I	CYCGRG	2 cut(s)	571, 590
AoxI	GGCC	5 cut(s)	176, 255, 299, 934, 1037
ApeKI	GCWGC	6 cut(s)	114, 428, 520, 1154, 1225, 1343
ApoI	RAATTY	2 cut(s)	78, 157
ArsI	GACNNNNNNTTYG	2 cut(s)	70, 102
AseI	ATTAAT	2 cut(s)	420, 1209
AsiGI	ACCGGT	1 cut(s)	384
AspLEI	GCGC	1 cut(s)	1348
AspS9I	GGNCC	2 cut(s)	223, 299
AsuC2I	CCSGG	2 cut(s)	297, 525
AsuHPI	GGTGA	3 cut(s)	427, 1161, 1228
AsuNHI	GCTAGC	1 cut(s)	833
AvaI	CYCGRG	2 cut(s)	571, 590
AvaII	GGWCC	1 cut(s)	223
AxyI	CCTNAGG	1 cut(s)	549
BalI	TGGCCA	1 cut(s)	936
BanI	GGYRCC	1 cut(s)	1451
BanII	GRGCYC	2 cut(s)	591, 1191
BarI	GAAGNNNNNNTAC	2 cut(s)	226, 258
BbsI	GAAGAC	2 cut(s)	80, 1271
Bbv12I	GWGCWC	1 cut(s)	591
BbvI	GCAGC	6 cut(s)	101, 415, 507, 1141, 1237, 1355
BccI	CCATC	3 cut(s)	719, 923, 1441
BceAI	ACGGC	1 cut(s)	1024
BcgI	CGANNNNNNTGC	2 cut(s)	649, 683
BciT130I	CCWGG	3 cut(s)	95, 1456, 1476
BciVI	GTATCC	1 cut(s)	1452
BclI	TGATCA	1 cut(s)	1576
BcnI	CCSGG	2 cut(s)	297, 525
BcoDI	GTCTC	4 cut(s)	190, 574, 768, 980
BfaI	CTAG	3 cut(s)	698, 834, 1271
BfoI	RGCGCY	1 cut(s)	1349
BfrI	CTTAAG	2 cut(s)	125, 1708
BfuAI	ACCTGC	1 cut(s)	661
BfuI	GTATCC	1 cut(s)	1452
BisI	GCNGC	6 cut(s)	115, 429, 521, 1155, 1226, 1344
BlsI	GCNGC	6 cut(s)	116, 430, 522, 1156, 1227, 1345
BmcAI	AGTACT	1 cut(s)	1130
Bme1390I	CCNGG	5 cut(s)	95, 297, 525, 1456, 1476
Bme18I	GGWCC	1 cut(s)	223
BmeT110I	CYCGRG	2 cut(s)	571, 590
BmgT120I	GGNCC	2 cut(s)	223, 299
BmiI	GGNNCC	4 cut(s)	300, 383, 1188, 1453
BmrFI	CCNGG	5 cut(s)	95, 297, 525, 1456, 1476
BmsI	GCATC	3 cut(s)	665, 1523, 1557
BmtI	GCTAGC	1 cut(s)	837
BoxI	GACNNNNGTC	1 cut(s)	1625
BpiI	GAAGAC	2 cut(s)	80, 1271
BpuMI	CCSGG	2 cut(s)	297, 525
Bsa29I	ATCGAT	1 cut(s)	659
BsaAI	YACGTR	2 cut(s)	900, 1589
BsaJI	CCNNGG	8 cut(s)	93, 296, 349, 524, 937, 1349, 1419, 1475
BsaWI	WCCGGW	1 cut(s)	384
BsaXI	ACNNNNNCTCC	2 cut(s)	454, 484
Bsc4I	CCNNNNNNNGG	5 cut(s)	355, 391, 524, 1504, 1713
Bse118I	RCCGGY	2 cut(s)	384, 1713
Bse1I	ACTGG	1 cut(s)	801
Bse21I	CCTNAGG	1 cut(s)	549
Bse3DI	GCAATG	1 cut(s)	1404
BseBI	CCWGG	3 cut(s)	95, 1456, 1476
BseCI	ATCGAT	1 cut(s)	659
BseDI	CCNNGG	8 cut(s)	93, 296, 349, 524, 937, 1349, 1419, 1475
BseGI	GGATG	4 cut(s)	337, 687, 765, 1168
BseLI	CCNNNNNNNGG	5 cut(s)	355, 391, 524, 1504, 1713
BseMI	GCAATG	1 cut(s)	1404
BseMII	CTCAG	5 cut(s)	31, 423, 563, 1029, 1672
BseNI	ACTGG	1 cut(s)	801
BseRI	GAGGAG	2 cut(s)	282, 475
BseXI	GCAGC	6 cut(s)	101, 415, 507, 1141, 1237, 1355
BseYI	CCCAGC	2 cut(s)	1008, 1262
BsgI	GTGCAG	1 cut(s)	1362
Bsh1236I	CGCG	1 cut(s)	1601
BshFI	GGCC	5 cut(s)	178, 257, 301, 936, 1039
BshNI	GGYRCC	1 cut(s)	1451
BshTI	ACCGGT	1 cut(s)	384
BshVI	ATCGAT	1 cut(s)	659
BsiHKAI	GWGCWC	1 cut(s)	591
BsiHKCI	CYCGRG	2 cut(s)	571, 590
BsiSI	CCGG	4 cut(s)	296, 385, 524, 1714
BslI	CCNNNNNNNGG	5 cut(s)	355, 391, 524, 1504, 1713
BsmAI	GTCTC	4 cut(s)	190, 574, 768, 980
BsmI	GAATGC	2 cut(s)	1227, 1266
BsnI	GGCC	5 cut(s)	178, 257, 301, 936, 1039
BsoBI	CYCGRG	2 cut(s)	571, 590
Bsp1286I	GDGCHC	2 cut(s)	591, 1191
Bsp143I	GATC	5 cut(s)	575, 903, 972, 1576, 1664
BspACI	CCGC	6 cut(s)	51, 477, 518, 709, 882, 1601
BspANI	GGCC	5 cut(s)	178, 257, 301, 936, 1039
BspCNI	CTCAG	5 cut(s)	30, 424, 562, 1028, 1671
BspDI	ATCGAT	1 cut(s)	659
BspFNI	CGCG	1 cut(s)	1601
BspLI	GGNNCC	4 cut(s)	300, 383, 1188, 1453
BspMI	ACCTGC	1 cut(s)	661
BspOI	GCTAGC	1 cut(s)	837
BspPI	GGATC	2 cut(s)	583, 911
BspT107I	GGYRCC	1 cut(s)	1451
BspTI	CTTAAG	2 cut(s)	125, 1708
BsrDI	GCAATG	1 cut(s)	1404
BsrFI	RCCGGY	2 cut(s)	384, 1713
BsrI	ACTGG	1 cut(s)	801
BssAI	RCCGGY	2 cut(s)	384, 1713
BssECI	CCNNGG	8 cut(s)	93, 296, 349, 524, 937, 1349, 1419, 1475
BssMI	GATC	5 cut(s)	575, 903, 972, 1576, 1664
BssT1I	CCWWGG	3 cut(s)	937, 1349, 1419
Bst2UI	CCWGG	3 cut(s)	95, 1456, 1476
Bst6I	CTCTTC	1 cut(s)	50
BstAFI	CTTAAG	2 cut(s)	125, 1708
BstAPI	GCANNNNNTGC	1 cut(s)	856
BstBAI	YACGTR	2 cut(s)	900, 1589
BstC8I	GCNNGC	2 cut(s)	835, 1146
BstDSI	CCRYGG	1 cut(s)	349
BstENI	CCTNNNNNAGG	1 cut(s)	1502
BstF5I	GGATG	4 cut(s)	337, 687, 765, 1168
BstFNI	CGCG	1 cut(s)	1601
BstH2I	RGCGCY	1 cut(s)	1349
BstHHI	GCGC	1 cut(s)	1348
BstKTI	GATC	5 cut(s)	578, 906, 975, 1579, 1667
BstMAI	GTCTC	4 cut(s)	190, 574, 768, 980
BstMBI	GATC	5 cut(s)	575, 903, 972, 1576, 1664
BstMWI	GCNNNNNNNGC	3 cut(s)	27, 856, 1154
BstNI	CCWGG	3 cut(s)	95, 1456, 1476
BstNSI	RCATGY	2 cut(s)	854, 1307
BstPAI	GACNNNNGTC	1 cut(s)	1625
BstSCI	CCNGG	5 cut(s)	93, 295, 523, 1454, 1474
BstSNI	TACGTA	1 cut(s)	1589
BstUI	CGCG	1 cut(s)	1601
BstV1I	GCAGC	6 cut(s)	101, 415, 507, 1141, 1237, 1355
BstV2I	GAAGAC	2 cut(s)	80, 1271
BstX2I	RGATCY	1 cut(s)	903
BstYI	RGATCY	1 cut(s)	903
Bsu15I	ATCGAT	1 cut(s)	659
Bsu36I	CCTNAGG	1 cut(s)	549
BsuI	GTATCC	1 cut(s)	1452
BsuRI	GGCC	5 cut(s)	178, 257, 301, 936, 1039
BsuTUI	ATCGAT	1 cut(s)	659
BtgI	CCRYGG	1 cut(s)	349
BtgZI	GCGATG	1 cut(s)	902
BtsCI	GGATG	4 cut(s)	337, 687, 765, 1168
BveI	ACCTGC	1 cut(s)	661
Cac8I	GCNNGC	2 cut(s)	835, 1146
CaiI	CAGNNNCTG	1 cut(s)	926
CfoI	GCGC	1 cut(s)	1348
Cfr10I	RCCGGY	2 cut(s)	384, 1713
Cfr13I	GGNCC	2 cut(s)	223, 299
ClaI	ATCGAT	1 cut(s)	659
Csp6I	GTAC	4 cut(s)	674, 1042, 1129, 1357
CspAI	ACCGGT	1 cut(s)	384
CspCI	CAANNNNNGTGG	2 cut(s)	247, 282
CviAII	CATG	2 cut(s)	851, 1304
CviQI	GTAC	4 cut(s)	674, 1042, 1129, 1357
DpnI	GATC	5 cut(s)	577, 905, 974, 1578, 1666
DpnII	GATC	5 cut(s)	575, 903, 972, 1576, 1664
DraIII	CACNNNGTG	1 cut(s)	1083
EaeI	YGGCCR	2 cut(s)	934, 1037
Eam1104I	CTCTTC	1 cut(s)	50
EarI	CTCTTC	1 cut(s)	50
Ecl136II	GAGCTC	1 cut(s)	589
Eco105I	TACGTA	1 cut(s)	1589
Eco130I	CCWWGG	3 cut(s)	937, 1349, 1419
Eco147I	AGGCCT	1 cut(s)	178
Eco24I	GRGCYC	2 cut(s)	591, 1191
Eco47I	GGWCC	1 cut(s)	223
Eco53kI	GAGCTC	1 cut(s)	589
Eco57I	CTGAAG	3 cut(s)	1109, 1122, 1200
Eco81I	CCTNAGG	1 cut(s)	549
Eco88I	CYCGRG	2 cut(s)	571, 590
EcoICRI	GAGCTC	1 cut(s)	589
EcoNI	CCTNNNNNAGG	1 cut(s)	1502
EcoRII	CCWGG	3 cut(s)	93, 1454, 1474
EcoT14I	CCWWGG	3 cut(s)	937, 1349, 1419
EcoT22I	ATGCAT	1 cut(s)	1375
EcoT38I	GRGCYC	2 cut(s)	591, 1191
ErhI	CCWWGG	3 cut(s)	937, 1349, 1419
FaeI	CATG	2 cut(s)	854, 1307
FalI	AAGNNNNNCTT	1 cut(s)	29
FatI	CATG	2 cut(s)	850, 1303
FauI	CCCGC	2 cut(s)	58, 1594
FbaI	TGATCA	1 cut(s)	1576
Fnu4HI	GCNGC	6 cut(s)	115, 429, 521, 1155, 1226, 1344
FokI	GGATG	4 cut(s)	344, 674, 772, 1175
FriOI	GRGCYC	2 cut(s)	591, 1191
Fsp4HI	GCNGC	6 cut(s)	115, 429, 521, 1155, 1226, 1344
FspBI	CTAG	3 cut(s)	698, 834, 1271
GlaI	GCGC	1 cut(s)	1347
GluI	GCNGC	6 cut(s)	115, 429, 521, 1155, 1226, 1344
GsaI	CCCAGC	2 cut(s)	1012, 1266
HaeII	RGCGCY	1 cut(s)	1349
HaeIII	GGCC	5 cut(s)	178, 257, 301, 936, 1039
HapII	CCGG	4 cut(s)	296, 385, 524, 1714
HhaI	GCGC	1 cut(s)	1348
Hin1II	CATG	2 cut(s)	854, 1307
Hin6I	GCGC	1 cut(s)	1346
HinP1I	GCGC	1 cut(s)	1346
HincII	GTYRAC	3 cut(s)	283, 1005, 1620
HindII	GTYRAC	3 cut(s)	283, 1005, 1620
HindIII	AAGCTT	3 cut(s)	1121, 1142, 1674
HinfI	GANTC	6 cut(s)	269, 344, 434, 636, 923, 1166
HpaII	CCGG	4 cut(s)	296, 385, 524, 1714
HphI	GGTGA	3 cut(s)	427, 1161, 1228
Hpy166II	GTNNAC	4 cut(s)	283, 1005, 1357, 1620
Hpy188I	TCNGA	4 cut(s)	70, 223, 233, 1661
Hpy188III	TCNNGA	6 cut(s)	445, 551, 573, 731, 927, 1017
Hpy8I	GTNNAC	4 cut(s)	283, 1005, 1357, 1620
HpyAV	CCTTC	6 cut(s)	23, 298, 531, 710, 1097, 1474
HpyCH4IV	ACGT	3 cut(s)	285, 899, 1588
HpyCH4V	TGCA	9 cut(s)	150, 670, 776, 964, 1157, 1225, 1303, 1343, 1373
HpyF10VI	GCNNNNNNNGC	3 cut(s)	27, 856, 1154
HpySE526I	ACGT	3 cut(s)	285, 899, 1588
Hsp92II	CATG	2 cut(s)	854, 1307
HspAI	GCGC	1 cut(s)	1346
Ksp22I	TGATCA	1 cut(s)	1576
Kzo9I	GATC	5 cut(s)	575, 903, 972, 1576, 1664
LmnI	GCTCC	3 cut(s)	26, 295, 1186
Lsp1109I	GCAGC	6 cut(s)	101, 415, 507, 1141, 1237, 1355
LweI	GCATC	3 cut(s)	665, 1523, 1557
MaeI	CTAG	3 cut(s)	698, 834, 1271
MaeII	ACGT	3 cut(s)	285, 899, 1588
MaeIII	GTNAC	3 cut(s)	472, 1075, 1096
MalI	GATC	5 cut(s)	577, 905, 974, 1578, 1666
MboI	GATC	5 cut(s)	575, 903, 972, 1576, 1664
MboII	GAAGA	6 cut(s)	37, 80, 748, 1126, 1271, 1674
MfeI	CAATTG	1 cut(s)	777
MflI	RGATCY	1 cut(s)	903
MhlI	GDGCHC	2 cut(s)	591, 1191
MlsI	TGGCCA	1 cut(s)	936
MluCI	AATT	9 cut(s)	78, 143, 157, 162, 227, 648, 777, 894, 1425
MluNI	TGGCCA	1 cut(s)	936
MlyI	GAGTC	2 cut(s)	443, 1160
Mox20I	TGGCCA	1 cut(s)	936
Mph1103I	ATGCAT	1 cut(s)	1375
MscI	TGGCCA	1 cut(s)	936
MslI	CAYNNNNRTG	1 cut(s)	203
Msp20I	TGGCCA	1 cut(s)	936
MspA1I	CMGCKG	2 cut(s)	114, 520
MspCI	CTTAAG	2 cut(s)	125, 1708
MspI	CCGG	4 cut(s)	296, 385, 524, 1714
MspR9I	CCNGG	5 cut(s)	95, 297, 525, 1456, 1476
MunI	CAATTG	1 cut(s)	777
Mva1269I	GAATGC	2 cut(s)	1227, 1266
MvaI	CCWGG	3 cut(s)	95, 1456, 1476
MvnI	CGCG	1 cut(s)	1601
MwoI	GCNNNNNNNGC	3 cut(s)	27, 856, 1154
NciI	CCSGG	2 cut(s)	297, 525
NdeII	GATC	5 cut(s)	575, 903, 972, 1576, 1664
NheI	GCTAGC	1 cut(s)	833
NlaIII	CATG	2 cut(s)	854, 1307
NlaIV	GGNNCC	4 cut(s)	300, 383, 1188, 1453
NmeAIII	GCCGAG	1 cut(s)	1716
NmuCI	GTSAC	2 cut(s)	472, 1075
NsiI	ATGCAT	1 cut(s)	1375
NspI	RCATGY	2 cut(s)	854, 1307
OliI	CACNNNNGTG	1 cut(s)	203
PceI	AGGCCT	1 cut(s)	178
PctI	GAATGC	2 cut(s)	1227, 1266
PfeI	GAWTC	4 cut(s)	269, 344, 636, 923
PinAI	ACCGGT	1 cut(s)	384
PkrI	GCNGC	6 cut(s)	116, 430, 522, 1156, 1227, 1345
PleI	GAGTC	2 cut(s)	442, 1160
PpsI	GAGTC	2 cut(s)	442, 1160
Ppu21I	YACGTR	2 cut(s)	900, 1589
PshAI	GACNNNNGTC	1 cut(s)	1625
PshBI	ATTAAT	2 cut(s)	420, 1209
PsiI	TTATAA	1 cut(s)	953
Psp124BI	GAGCTC	1 cut(s)	591
Psp6I	CCWGG	3 cut(s)	93, 1454, 1474
PspFI	CCCAGC	2 cut(s)	1008, 1262
PspGI	CCWGG	3 cut(s)	93, 1454, 1474
PspN4I	GGNNCC	4 cut(s)	300, 383, 1188, 1453
PspPI	GGNCC	2 cut(s)	223, 299
PstNI	CAGNNNCTG	1 cut(s)	926
PsuI	RGATCY	1 cut(s)	903
PvuII	CAGCTG	1 cut(s)	114
RsaI	GTAC	4 cut(s)	675, 1043, 1130, 1358
RsaNI	GTAC	4 cut(s)	674, 1042, 1129, 1357
RseI	CAYNNNNRTG	1 cut(s)	203
SacI	GAGCTC	1 cut(s)	591
SatI	GCNGC	6 cut(s)	115, 429, 521, 1155, 1226, 1344
Sau3AI	GATC	5 cut(s)	575, 903, 972, 1576, 1664
Sau96I	GGNCC	2 cut(s)	223, 299
ScaI	AGTACT	1 cut(s)	1130
SchI	GAGTC	2 cut(s)	443, 1160
ScrFI	CCNGG	5 cut(s)	95, 297, 525, 1456, 1476
SduI	GDGCHC	2 cut(s)	591, 1191
SfaNI	GCATC	3 cut(s)	665, 1523, 1557
SinI	GGWCC	1 cut(s)	223
SmiMI	CAYNNNNRTG	1 cut(s)	203
SmlI	CTYRAG	2 cut(s)	125, 1708
SmoI	CTYRAG	2 cut(s)	125, 1708
SnaBI	TACGTA	1 cut(s)	1589
Sse9I	AATT	9 cut(s)	78, 143, 157, 162, 227, 648, 777, 894, 1425
SseBI	AGGCCT	1 cut(s)	178
SsiI	CCGC	6 cut(s)	51, 477, 518, 709, 882, 1601
SspI	AATATT	1 cut(s)	969
SspMI	CTAG	3 cut(s)	698, 834, 1271
SstI	GAGCTC	1 cut(s)	591
StuI	AGGCCT	1 cut(s)	178
StyD4I	CCNGG	5 cut(s)	93, 295, 523, 1454, 1474
StyI	CCWWGG	3 cut(s)	937, 1349, 1419
TaiI	ACGT	3 cut(s)	288, 902, 1591
TaqI	TCGA	4 cut(s)	61, 659, 1646, 1653
TasI	AATT	9 cut(s)	78, 143, 157, 162, 227, 648, 777, 894, 1425
TatI	WGTACW	2 cut(s)	1128, 1356
TfiI	GAWTC	4 cut(s)	269, 344, 636, 923
TseFI	GTSAC	2 cut(s)	472, 1075
TseI	GCWGC	6 cut(s)	114, 428, 520, 1154, 1225, 1343
Tsp45I	GTSAC	2 cut(s)	472, 1075
TspDTI	ATGAA	2 cut(s)	933, 1278
Vha464I	CTTAAG	2 cut(s)	125, 1708
VpaK11BI	GGWCC	1 cut(s)	223
VspI	ATTAAT	2 cut(s)	420, 1209
XagI	CCTNNNNNAGG	1 cut(s)	1502
XapI	RAATTY	2 cut(s)	78, 157
XceI	RCATGY	2 cut(s)	854, 1307
XspI	CTAG	3 cut(s)	698, 834, 1271
ZrmI	AGTACT	1 cut(s)	1130
Zsp2I	ATGCAT	1 cut(s)	1375

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.