RchiOBHm_Chr6g0291131

Belongs to the SecY SEC61-alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008295
Physical Location & Seq
Forward (+)
54323282 .. 54329213
5932 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26123

Sequence Viewer

Length: 1728 bp
ATGGAAGCCACTCTTCTCAGCTCTCAACGCTTCCAGCCCCAACTCTTCCCCTCTAAATTTCGAGGTTCTGATACTCCAATTTGTTGCCCACGCTTAGTTAGAACCAAAGCCAACTTTGTTTCCCTCAAGACCAATCCTTCACAATTATGCAGCACAAATTCAATTTTGGCAAGCAGGTCTTTATTCTCCAGAGTAAGCACAAGAGTGTCTGCTAAGTTTTCGAACCAATTCCGAAATGAATATACCGATGTTGAGGCCACAGCTGCAGAATCTTTAAATCTTGAAGTTGTTCCGCCGAGGCCGAATGGCGGGAGAGGTGTTGTTACGCAGGATGAGATTGGCGACTCCGGTGGTGCGGAGGCGAGACCTGTAATGTTTAGGAACAGGTTTCTGGATTTTGTGAGGATAAGTTCAGTGATTAATAATGCTGCTGAGTCGTTCTTCAAGAGTGAGATTAGGAGGAGGTTGTTTGTGACGGCAGTGCTGATTGTGATTAGTCGTGTCGGTTATTTCATTCCTCTGCCTGGGTTTGATAGAAGGTTGATACCTCAGGATTATCTCAGCTTTGTCTCGGGATCAGTTGATGAGCTCGGCGATTTTACAGCAGAGCTTAAAATGTCCTTGTTCCAGCTTGGAATCAGTCCTCAAATTATAGCATCGATTATCATGCAGGTACTCTGTTATGTCGTCCCCTCCCTAGTAAAGCTGCGGAAAGAAGGCTTAGATGGTCAAGAGAAGATTAAGAGTTATATATGGTGGATGTCTCTTGGCTTTGCAATTGTGGAGGCTGTGATACTTGCTTGTTATTCACTTCCCTATTCAATCTATGTAGCTAGCCACAGGGTCAAGCATGTGATGCTGACAAGTATGTTATTGGTCTGTGGTGCAATGACAATGACGTGGATATGTGATACCATTACAGAATCTGGATTCGGCCAAGGTTCATCTTTAATAATATGTGTGCAAATATTGATTGGCTACACAGAGACATTATACAAAATGTTGACCCAGCTTTCAGGAAGTTCTGTAAGTTGGTGGCCATACTTATTTGGAGTATTGGGCGTTTTCACTATAGTCACTATGTGGGCAGTTGTGGTAACTGAAGGATGCAGGAAGATAAAGCTTCAGTACTACGGTTTTAAGCTGGCTTCTGCTGCAAGGGATGACTCACCTATTACTGAAGTGGAGCCCTATATACCTTTTACTATTAATCCAGCAGGAATGCAGCCTGTTCTCACAACTACTTATCTCTTGGCATTTCCCAGCATTCTTGCAAGTCTTCTTGGTTCGTCTTTCTGGGAGCATGTTAAGGAGATATTGAACCCTGATACTTCTATTGGTGCAGCGCCTTGGGTGTACTACACAATATATGCGTTTTTTGTCTTTTTATTCAATATATTTGACATAGCCAACTTGCCCAAGGAAATTGCTGACTACTTAAATAAGATGGGTGCAAGGATACCAAATATAAAACCTGGAAAGGCTACGATTGAATATCTCACAAAGATTCAGGCATCAACACGTTTTTGGGGTGGTGTATTGTTAAGCATCTTAGCAACAACATCAAGCATACTTGATCATTATTTACGTAACATCAATGCGGGATTTTCAATCGGGTTGACATCAGTCTTAATAATAGTGGGTTCGATTATTGAACTTAGAAGATCATATCAAGCATATAATGTGATGCCAAGTTTAAGTAAAGCCTTAAGACGGTACGGTGTGTAG

Protein Analysis

575

Amino Acids

64.04

Weight (kDa)

9.15

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecY PF00344 207 - 552 3.7e-69 SecY
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 165, 661
AciI CCGC 5 cut(s) 293, 309, 356, 709, 1601
AclWI GGATC 1 cut(s) 583
AcoI YGGCCR 2 cut(s) 934, 1037
AcsI RAATTY 2 cut(s) 56, 157
AcuI CTGAAG 3 cut(s) 1109, 1122, 1200
AdeI CACNNNGTG 1 cut(s) 1083
AfaI GTAC 4 cut(s) 675, 1130, 1358, 1718
AfiI CCNNNNNNNGG 3 cut(s) 308, 524, 1713
AflII CTTAAG 1 cut(s) 1708
AflIII ACRYGT 1 cut(s) 1520
AgsI TTSAA 9 cut(s) 162, 284, 445, 822, 1321, 1393, 1493, 1611, 1655
AjiI CACGTC 1 cut(s) 900
AjnI CCWGG 2 cut(s) 523, 1474
AleI CACNNNNGTG 1 cut(s) 203
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 4 cut(s) 358, 574, 768, 980
AlwI GGATC 1 cut(s) 583
AlwNI CAGNNNCTG 1 cut(s) 926
Ama87I CYCGRG 1 cut(s) 571
AoxI GGCC 4 cut(s) 255, 299, 934, 1037
ApeKI GCWGC 7 cut(s) 150, 263, 428, 706, 1154, 1225, 1343
ApoI RAATTY 2 cut(s) 56, 157
AseI ATTAAT 2 cut(s) 420, 1209
Asp700I GAANNNNTTC 2 cut(s) 227, 288
AspLEI GCGC 1 cut(s) 1348
AsuHPI GGTGA 1 cut(s) 1161
AsuII TTCGAA 1 cut(s) 221
AsuNHI GCTAGC 1 cut(s) 833
AvaI CYCGRG 1 cut(s) 571
AxyI CCTNAGG 1 cut(s) 549
BalI TGGCCA 1 cut(s) 1039
BanII GRGCYC 2 cut(s) 591, 1191
BbsI GAAGAC 1 cut(s) 1271
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 7 cut(s) 162, 250, 415, 693, 1141, 1237, 1355
BccI CCATC 2 cut(s) 719, 1441
BceAI ACGGC 1 cut(s) 492
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 2 cut(s) 525, 1476
BciVI GTATCC 1 cut(s) 1452
BclI TGATCA 1 cut(s) 1576
BcoDI GTCTC 4 cut(s) 358, 574, 768, 980
BfaI CTAG 2 cut(s) 698, 834
BfmI CTRYAG 2 cut(s) 264, 1071
BfoI RGCGCY 1 cut(s) 1349
BfrI CTTAAG 1 cut(s) 1708
BfuAI ACCTGC 2 cut(s) 165, 661
BfuI GTATCC 1 cut(s) 1452
BisI GCNGC 7 cut(s) 151, 264, 429, 707, 1155, 1226, 1344
BlsI GCNGC 7 cut(s) 152, 265, 430, 708, 1156, 1227, 1345
BmcAI AGTACT 1 cut(s) 1130
Bme1390I CCNGG 2 cut(s) 525, 1476
BmeT110I CYCGRG 1 cut(s) 571
BmgBI CACGTC 1 cut(s) 900
BmiI GGNNCC 1 cut(s) 1188
BmrFI CCNGG 2 cut(s) 525, 1476
BmsI GCATC 6 cut(s) 665, 846, 1097, 1523, 1557, 1677
BmtI GCTAGC 1 cut(s) 837
BoxI GACNNNNGTC 1 cut(s) 1625
BpiI GAAGAC 1 cut(s) 1271
BpmI CTGGAG 1 cut(s) 172
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 1 cut(s) 110
Bsa29I ATCGAT 1 cut(s) 659
BsaAI YACGTR 1 cut(s) 1589
BsaI GGTCTC 1 cut(s) 358
BsaJI CCNNGG 5 cut(s) 296, 524, 937, 1349, 1419
BsaWI WCCGGW 1 cut(s) 347
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 524, 1713
Bse21I CCTNAGG 1 cut(s) 549
Bse3DI GCAATG 1 cut(s) 894
BseBI CCWGG 2 cut(s) 525, 1476
BseCI ATCGAT 1 cut(s) 659
BseDI CCNNGG 5 cut(s) 296, 524, 937, 1349, 1419
BseGI GGATG 4 cut(s) 337, 765, 1112, 1168
BseLI CCNNNNNNNGG 3 cut(s) 308, 524, 1713
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 4 cut(s) 31, 423, 563, 574
BseRI GAGGAG 1 cut(s) 475
BseXI GCAGC 7 cut(s) 162, 250, 415, 693, 1141, 1237, 1355
BseYI CCCAGC 2 cut(s) 1008, 1262
BsgI GTGCAG 1 cut(s) 1362
BshFI GGCC 4 cut(s) 257, 301, 936, 1039
BshVI ATCGAT 1 cut(s) 659
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 571
BsiSI CCGG 1 cut(s) 348
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 3 cut(s) 308, 524, 1713
BsmAI GTCTC 4 cut(s) 358, 574, 768, 980
BsmFI GGGAC 1 cut(s) 674
BsmI GAATGC 2 cut(s) 1227, 1266
BsnI GGCC 4 cut(s) 257, 301, 936, 1039
Bso31I GGTCTC 1 cut(s) 358
BsoBI CYCGRG 1 cut(s) 571
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 591, 1191
Bsp143I GATC 3 cut(s) 575, 1576, 1664
BspACI CCGC 5 cut(s) 293, 309, 356, 709, 1601
BspANI GGCC 4 cut(s) 257, 301, 936, 1039
BspCNI CTCAG 4 cut(s) 30, 424, 562, 573
BspDI ATCGAT 1 cut(s) 659
BspLI GGNNCC 1 cut(s) 1188
BspMAI CTGCAG 1 cut(s) 268
BspMI ACCTGC 2 cut(s) 165, 661
BspOI GCTAGC 1 cut(s) 837
BspPI GGATC 1 cut(s) 583
BspT104I TTCGAA 1 cut(s) 221
BspTI CTTAAG 1 cut(s) 1708
BspTNI GGTCTC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 894
BssECI CCNNGG 5 cut(s) 296, 524, 937, 1349, 1419
BssMI GATC 3 cut(s) 575, 1576, 1664
BssT1I CCWWGG 3 cut(s) 937, 1349, 1419
Bst2UI CCWGG 2 cut(s) 525, 1476
Bst4CI ACNGT 3 cut(s) 1136, 1716, 1721
Bst6I CTCTTC 2 cut(s) 18, 50
BstAFI CTTAAG 1 cut(s) 1708
BstAPI GCANNNNNTGC 1 cut(s) 856
BstBAI YACGTR 1 cut(s) 1589
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 3 cut(s) 172, 835, 1146
BstDEI CTNAG 9 cut(s) 17, 94, 213, 432, 549, 560, 721, 1552, 1658
BstF5I GGATG 4 cut(s) 337, 765, 1112, 1168
BstH2I RGCGCY 1 cut(s) 1349
BstHHI GCGC 1 cut(s) 1348
BstKTI GATC 3 cut(s) 578, 1579, 1667
BstMAI GTCTC 4 cut(s) 358, 574, 768, 980
BstMBI GATC 3 cut(s) 575, 1576, 1664
BstMWI GCNNNNNNNGC 4 cut(s) 27, 263, 856, 1154
BstNI CCWGG 2 cut(s) 525, 1476
BstNSI RCATGY 2 cut(s) 854, 1307
BstPAI GACNNNNGTC 1 cut(s) 1625
BstSCI CCNGG 2 cut(s) 523, 1474
BstSFI CTRYAG 2 cut(s) 264, 1071
BstSNI TACGTA 1 cut(s) 1589
BstV1I GCAGC 7 cut(s) 162, 250, 415, 693, 1141, 1237, 1355
BstV2I GAAGAC 1 cut(s) 1271
Bsu15I ATCGAT 1 cut(s) 659
Bsu36I CCTNAGG 1 cut(s) 549
BsuI GTATCC 1 cut(s) 1452
BsuRI GGCC 4 cut(s) 257, 301, 936, 1039
BsuTUI ATCGAT 1 cut(s) 659
BtrI CACGTC 1 cut(s) 900
BtsCI GGATG 4 cut(s) 337, 765, 1112, 1168
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 420, 486
BveI ACCTGC 2 cut(s) 165, 661
Cac8I GCNNGC 3 cut(s) 172, 835, 1146
CaiI CAGNNNCTG 1 cut(s) 926
CfoI GCGC 1 cut(s) 1348
ClaI ATCGAT 1 cut(s) 659
Csp6I GTAC 4 cut(s) 674, 1129, 1357, 1717
CviAII CATG 3 cut(s) 667, 851, 1304
CviQI GTAC 4 cut(s) 674, 1129, 1357, 1717
DdeI CTNAG 9 cut(s) 17, 94, 213, 432, 549, 560, 721, 1552, 1658
DpnI GATC 3 cut(s) 577, 1578, 1666
DpnII GATC 3 cut(s) 575, 1576, 1664
DraI TTTAAA 1 cut(s) 276
DraIII CACNNNGTG 1 cut(s) 1083
EaeI YGGCCR 2 cut(s) 934, 1037
Eam1104I CTCTTC 2 cut(s) 18, 50
EarI CTCTTC 2 cut(s) 18, 50
EciI GGCGGA 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 589
Eco105I TACGTA 1 cut(s) 1589
Eco130I CCWWGG 3 cut(s) 937, 1349, 1419
Eco24I GRGCYC 2 cut(s) 591, 1191
Eco31I GGTCTC 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 589
Eco57I CTGAAG 3 cut(s) 1109, 1122, 1200
Eco81I CCTNAGG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 571
EcoICRI GAGCTC 1 cut(s) 589
EcoRII CCWGG 2 cut(s) 523, 1474
EcoT14I CCWWGG 3 cut(s) 937, 1349, 1419
EcoT38I GRGCYC 2 cut(s) 591, 1191
ErhI CCWWGG 3 cut(s) 937, 1349, 1419
FaeI CATG 3 cut(s) 670, 854, 1307
FalI AAGNNNNNCTT 3 cut(s) 29, 163, 195
FaqI GGGAC 1 cut(s) 674
FatI CATG 3 cut(s) 666, 850, 1303
FauI CCCGC 2 cut(s) 302, 1594
FbaI TGATCA 1 cut(s) 1576
Fnu4HI GCNGC 7 cut(s) 151, 264, 429, 707, 1155, 1226, 1344
FokI GGATG 4 cut(s) 344, 772, 1119, 1175
FriOI GRGCYC 2 cut(s) 591, 1191
Fsp4HI GCNGC 7 cut(s) 151, 264, 429, 707, 1155, 1226, 1344
FspBI CTAG 2 cut(s) 698, 834
GlaI GCGC 1 cut(s) 1347
GluI GCNGC 7 cut(s) 151, 264, 429, 707, 1155, 1226, 1344
GsaI CCCAGC 2 cut(s) 1012, 1266
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 1 cut(s) 1349
HaeIII GGCC 4 cut(s) 257, 301, 936, 1039
HapII CCGG 1 cut(s) 348
HhaI GCGC 1 cut(s) 1348
Hin1II CATG 3 cut(s) 670, 854, 1307
Hin6I GCGC 1 cut(s) 1346
HinP1I GCGC 1 cut(s) 1346
HincII GTYRAC 2 cut(s) 1005, 1620
HindII GTYRAC 2 cut(s) 1005, 1620
HindIII AAGCTT 1 cut(s) 1121
HinfI GANTC 8 cut(s) 269, 344, 434, 636, 923, 930, 1166, 1507
HpaII CCGG 1 cut(s) 348
HphI GGTGA 1 cut(s) 1161
Hpy166II GTNNAC 3 cut(s) 1005, 1357, 1620
Hpy188I TCNGA 2 cut(s) 70, 233
Hpy8I GTNNAC 3 cut(s) 1005, 1357, 1620
HpyAV CCTTC 4 cut(s) 147, 531, 710, 1097
HpyCH4III ACNGT 3 cut(s) 1136, 1716, 1721
HpyCH4IV ACGT 3 cut(s) 899, 1522, 1588
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 263, 856, 1154
HpyF3I CTNAG 9 cut(s) 17, 94, 213, 432, 549, 560, 721, 1552, 1658
HpySE526I ACGT 3 cut(s) 899, 1522, 1588
Hsp92II CATG 3 cut(s) 670, 854, 1307
HspAI GCGC 1 cut(s) 1346
Ksp22I TGATCA 1 cut(s) 1576
Kzo9I GATC 3 cut(s) 575, 1576, 1664
LmnI GCTCC 2 cut(s) 1186, 1300
Lsp1109I GCAGC 7 cut(s) 162, 250, 415, 693, 1141, 1237, 1355
LweI GCATC 6 cut(s) 665, 846, 1097, 1523, 1557, 1677
MaeI CTAG 2 cut(s) 698, 834
MaeII ACGT 3 cut(s) 899, 1522, 1588
MaeIII GTNAC 5 cut(s) 322, 472, 1075, 1096, 1589
MalI GATC 3 cut(s) 577, 1578, 1666
MboI GATC 3 cut(s) 575, 1576, 1664
MboII GAAGA 7 cut(s) 5, 37, 433, 748, 1126, 1271, 1674
MfeI CAATTG 1 cut(s) 777
MhlI GDGCHC 2 cut(s) 591, 1191
MlsI TGGCCA 1 cut(s) 1039
MluCI AATT 9 cut(s) 56, 78, 143, 157, 162, 227, 648, 777, 1425
MluNI TGGCCA 1 cut(s) 1039
MlyI GAGTC 3 cut(s) 338, 443, 1160
Mox20I TGGCCA 1 cut(s) 1039
MroXI GAANNNNTTC 2 cut(s) 227, 288
MscI TGGCCA 1 cut(s) 1039
MslI CAYNNNNRTG 2 cut(s) 145, 203
Msp20I TGGCCA 1 cut(s) 1039
MspA1I CMGCKG 1 cut(s) 263
MspCI CTTAAG 1 cut(s) 1708
MspI CCGG 1 cut(s) 348
MspR9I CCNGG 2 cut(s) 525, 1476
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 2 cut(s) 1227, 1266
MvaI CCWGG 2 cut(s) 525, 1476
MwoI GCNNNNNNNGC 4 cut(s) 27, 263, 856, 1154
NdeII GATC 3 cut(s) 575, 1576, 1664
NheI GCTAGC 1 cut(s) 833
NlaIII CATG 3 cut(s) 670, 854, 1307
NlaIV GGNNCC 1 cut(s) 1188
NmeAIII GCCGAG 2 cut(s) 321, 570
NmuCI GTSAC 2 cut(s) 472, 1075
NspI RCATGY 2 cut(s) 854, 1307
NspV TTCGAA 1 cut(s) 221
OliI CACNNNNGTG 1 cut(s) 203
PctI GAATGC 2 cut(s) 1227, 1266
PdmI GAANNNNTTC 2 cut(s) 227, 288
PfeI GAWTC 5 cut(s) 269, 636, 923, 930, 1507
PkrI GCNGC 7 cut(s) 152, 265, 430, 708, 1156, 1227, 1345
PleI GAGTC 3 cut(s) 338, 442, 1160
PpsI GAGTC 3 cut(s) 338, 442, 1160
Ppu21I YACGTR 1 cut(s) 1589
PshAI GACNNNNGTC 1 cut(s) 1625
PshBI ATTAAT 2 cut(s) 420, 1209
Psp124BI GAGCTC 1 cut(s) 591
Psp6I CCWGG 2 cut(s) 523, 1474
PspFI CCCAGC 2 cut(s) 1008, 1262
PspGI CCWGG 2 cut(s) 523, 1474
PspN4I GGNNCC 1 cut(s) 1188
PstI CTGCAG 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 926
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 4 cut(s) 675, 1130, 1358, 1718
RsaNI GTAC 4 cut(s) 674, 1129, 1357, 1717
RseI CAYNNNNRTG 2 cut(s) 145, 203
SacI GAGCTC 1 cut(s) 591
SatI GCNGC 7 cut(s) 151, 264, 429, 707, 1155, 1226, 1344
Sau3AI GATC 3 cut(s) 575, 1576, 1664
ScaI AGTACT 1 cut(s) 1130
SchI GAGTC 3 cut(s) 338, 443, 1160
ScrFI CCNGG 2 cut(s) 525, 1476
SduI GDGCHC 2 cut(s) 591, 1191
SfaNI GCATC 6 cut(s) 665, 846, 1097, 1523, 1557, 1677
SfcI CTRYAG 2 cut(s) 264, 1071
SfuI TTCGAA 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 145, 203
SmlI CTYRAG 2 cut(s) 125, 1708
SmoI CTYRAG 2 cut(s) 125, 1708
SnaBI TACGTA 1 cut(s) 1589
Sse9I AATT 9 cut(s) 56, 78, 143, 157, 162, 227, 648, 777, 1425
SsiI CCGC 5 cut(s) 293, 309, 356, 709, 1601
SspI AATATT 1 cut(s) 969
SspMI CTAG 2 cut(s) 698, 834
SstI GAGCTC 1 cut(s) 591
StyD4I CCNGG 2 cut(s) 523, 1474
StyI CCWWGG 3 cut(s) 937, 1349, 1419
TaaI ACNGT 3 cut(s) 1136, 1716, 1721
TaiI ACGT 3 cut(s) 902, 1525, 1591
TaqI TCGA 4 cut(s) 61, 221, 659, 1646
TasI AATT 9 cut(s) 56, 78, 143, 157, 162, 227, 648, 777, 1425
TatI WGTACW 2 cut(s) 1128, 1356
TfiI GAWTC 5 cut(s) 269, 636, 923, 930, 1507
TscAI CASTG 2 cut(s) 420, 486
TseFI GTSAC 2 cut(s) 472, 1075
TseI GCWGC 7 cut(s) 150, 263, 428, 706, 1154, 1225, 1343
Tsp45I GTSAC 2 cut(s) 472, 1075
TspDTI ATGAA 3 cut(s) 252, 502, 933
TspRI CASTG 2 cut(s) 420, 486
Vha464I CTTAAG 1 cut(s) 1708
VspI ATTAAT 2 cut(s) 420, 1209
XapI RAATTY 2 cut(s) 56, 157
XceI RCATGY 2 cut(s) 854, 1307
XmnI GAANNNNTTC 2 cut(s) 227, 288
XspI CTAG 2 cut(s) 698, 834
ZrmI AGTACT 1 cut(s) 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.