Rroxscaffold_7G00176350

Belongs to the SecY SEC61-alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008295
Physical Location & Seq
Reverse (-)
15814572 .. 15820608
6037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00176350.1

Sequence Viewer

Length: 1911 bp
ATGGAAGCCACTGTTCTCAGCTCTCAACGCTTCCACCCCCAACTCTTCCCCTCTAAATTTCGAGGTTCTGATACTCCATTTTGTTGCCCACCCTTAGTTAGAACCAAAGCCAACTTTGTTTCCCTTAAGACCAATCCTTCACAATTATGCACCACAAATTCAATTTTCCCAAGCTGGTCTTTATTCTCCAGAGTAAGCACAAGAGTGTCTGCTAAGTTTTCGAACCAATTCCAAAATGAATATACCGATGTTGAGGCCACAGCTGCAGAATCTCTAAATCTTGAAGTTGCTCCGCCGAGGCCGAATGGCGGGAGCGTTGTTGTTACGCCGGATGAGATTGGCGACTCCGGAGTTGCGGAGGCGAAACCTGTAATGTTTAGGAACAGGTTTCTGGATTTTGTGAGGATAAGTTCAGTGATTAATAATGCTGCTGAGTCGTTTTTCAAAAGTGAGATTCGGAGGAGGTTGTTTGTGACGGCAGTGCTGATTGTGATTAGTCGTGTTGGTTATTTCATTCCTCTGCCTGGGTTTGATAGAAGGTTGATACCTCAGGATTATCTCAGCTTTGTCTCGGGATCAGTTGATGAGCTTGGCGATTTTACAGCAGAGCTTAAAATGTCCTTGTTCCAGCTTGGAATCAGTCCTCAAATTATAGCGTCGATTATCATGCAGGTACTCTGTTATGTCGTCCCCTCCCTAGTAAAGCTGCGGAAAGAAGGCTTAGATGGTCAAGAGAAGATTAAGAGTTATATATGGTGGATGTCTCTTGGCTTTGCAATTGTGGAGGCTCTGATACTTGCTTGTTATTCACTTCCCTATTCAATCTATGTAGCTAGCCACAGGGTCAAGCATGTGATGCTGACAAGTATGTTATTGGTCTGTGGTGCAATGACAATGACGTGGATATGTGATACCATTACAGAATCTGGATTCGGCCAAGGTTCATCTTTAATAATATGTGTGCAAATATTGATTGGCTACACAGAGACATTATACAAAATGTTGACCCAGCTTTCGGGAAGTTCTGTTAGTTGGTGGCCATACTTATTTGGAGTATTGGGCGTTTTCACTATAGTCACTATGTGGGCAGTTGTGGTAACTGAAGGATGTAGGAAGATAAAGCTTCAGTACTACGGTTTTAAGCTTGCTTCTGCTGCAAGGGATGACTCACCTATTACTGAAGTGGAGCCCTATATACCTTTTACTATTAATCCAGCAGGAATGCAGCCTGTTCTCACAACTGCTTATCTCTTGGCATTTCCCAGCATTCTTGCAAGTCTTCTTGGTTCGTCTTTCTGGGAGCATGTTAAGGAGATATTGAACCCTGATTCTTCTATTGGTGCGGCGCCTTGGGTGTACTACACAATATATGCCTTTTTTGTCTTTTTATTCAATATATTTGACATAGCCAACTTGCCCAAGGAAATTGCTGACTACTTAAATAAGATGGGTGCAAGGATACCAAATATAAAACCTGGAAAGGCTACGATTGAATATCTCACAAAGATGCAGGCATCGACACGTTTTTGGGGTGGTGTATTGTTAAGCATCTTAGCAACAACATCAAGCATACTTGATCATTATTTACGTAACATCAATGCGGGATTTTCAATCGGGTTGACATCAGTCTTAATAATAGTGGGTTCGATTATTGAACTTAGAAGATCATATCAAGCATATAATGTGATGCCAAGTTTAAGTAAAGCCTTAAGACGGTACGTGCTGGAATTCGACCTGCAGCAGAAAGGGTTCTTACATTACGATCTGGTTCAAGGGGTCTGTACTGGTGGTTTTGAGCAGCAGCTAATACCAAGGTCATCGGAAAATACATACACAAGACTCATCGCCAGCATGGTTTCTGTATCTTATGGAAACGGTTATGTTTCCGTTAGTTCATCAAAGCAACCCTAA

Protein Analysis

636

Amino Acids

70.79

Weight (kDa)

8.71

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecY PF00344 207 - 552 9.2e-69 SecY
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 661, 1743
AccB1I GGYRCC 1 cut(s) 1345
AccIII TCCGGA 1 cut(s) 347
AciI CCGC 6 cut(s) 293, 309, 356, 709, 1343, 1601
AclWI GGATC 1 cut(s) 583
AcoI YGGCCR 2 cut(s) 934, 1037
AcsI RAATTY 3 cut(s) 56, 157, 1727
AcuI CTGAAG 3 cut(s) 1109, 1122, 1200
AcyI GRCGYC 1 cut(s) 1346
AdeI CACNNNGTG 1 cut(s) 1083
AfaI GTAC 5 cut(s) 675, 1130, 1358, 1718, 1783
AfiI CCNNNNNNNGG 4 cut(s) 308, 524, 1015, 1713
AflII CTTAAG 2 cut(s) 125, 1708
AflIII ACRYGT 1 cut(s) 1520
AjiI CACGTC 1 cut(s) 900
AjnI CCWGG 2 cut(s) 523, 1474
AleI CACNNNNGTG 1 cut(s) 203
Alw26I GTCTC 3 cut(s) 574, 768, 980
AlwI GGATC 1 cut(s) 583
AlwNI CAGNNNCTG 1 cut(s) 926
Ama87I CYCGRG 1 cut(s) 571
Aor13HI TCCGGA 1 cut(s) 347
AoxI GGCC 4 cut(s) 255, 299, 934, 1037
ApeKI GCWGC 8 cut(s) 263, 428, 706, 1154, 1225, 1738, 1798, 1801
ApoI RAATTY 3 cut(s) 56, 157, 1727
ArsI GACNNNNNNTTYG 2 cut(s) 997, 1029
AseI ATTAAT 2 cut(s) 420, 1209
Asp700I GAANNNNTTC 2 cut(s) 227, 1748
AspLEI GCGC 1 cut(s) 1348
AsuHPI GGTGA 1 cut(s) 1161
AsuII TTCGAA 1 cut(s) 221
AsuNHI GCTAGC 1 cut(s) 833
AvaI CYCGRG 1 cut(s) 571
AxyI CCTNAGG 1 cut(s) 549
BalI TGGCCA 1 cut(s) 1039
BanI GGYRCC 1 cut(s) 1345
BanII GRGCYC 1 cut(s) 1191
BbsI GAAGAC 1 cut(s) 1271
BbvI GCAGC 8 cut(s) 250, 415, 693, 1141, 1237, 1750, 1810, 1813
BccI CCATC 2 cut(s) 719, 1441
BceAI ACGGC 1 cut(s) 492
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 2 cut(s) 525, 1476
BciVI GTATCC 1 cut(s) 1452
BclI TGATCA 1 cut(s) 1576
BcoDI GTCTC 3 cut(s) 574, 768, 980
BfaI CTAG 2 cut(s) 698, 834
BfmI CTRYAG 3 cut(s) 264, 1071, 1736
BfoI RGCGCY 1 cut(s) 1349
BfrI CTTAAG 2 cut(s) 125, 1708
BfuAI ACCTGC 2 cut(s) 661, 1743
BfuI GTATCC 1 cut(s) 1452
BisI GCNGC 9 cut(s) 264, 429, 707, 1155, 1226, 1344, 1739, 1799, 1802
BlsI GCNGC 9 cut(s) 265, 430, 708, 1156, 1227, 1345, 1740, 1800, 1803
BmcAI AGTACT 1 cut(s) 1130
Bme1390I CCNGG 2 cut(s) 525, 1476
BmeT110I CYCGRG 1 cut(s) 571
BmgBI CACGTC 1 cut(s) 900
BmiI GGNNCC 2 cut(s) 1188, 1347
BmrFI CCNGG 2 cut(s) 525, 1476
BmsI GCATC 5 cut(s) 846, 1497, 1523, 1557, 1677
BmtI GCTAGC 1 cut(s) 837
BoxI GACNNNNGTC 1 cut(s) 1625
BpiI GAAGAC 1 cut(s) 1271
BpmI CTGGAG 1 cut(s) 172
Bpu14I TTCGAA 1 cut(s) 221
BsaAI YACGTR 2 cut(s) 1589, 1720
BsaHI GRCGYC 1 cut(s) 1346
BsaJI CCNNGG 6 cut(s) 296, 524, 937, 1349, 1419, 1811
BsaWI WCCGGW 1 cut(s) 347
Bsc4I CCNNNNNNNGG 4 cut(s) 308, 524, 1015, 1713
Bse1I ACTGG 1 cut(s) 1789
Bse21I CCTNAGG 1 cut(s) 549
Bse3DI GCAATG 1 cut(s) 894
BseAI TCCGGA 1 cut(s) 347
BseBI CCWGG 2 cut(s) 525, 1476
BseDI CCNNGG 6 cut(s) 296, 524, 937, 1349, 1419, 1811
BseGI GGATG 4 cut(s) 337, 765, 1112, 1168
BseLI CCNNNNNNNGG 4 cut(s) 308, 524, 1015, 1713
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 4 cut(s) 31, 423, 563, 574
BseNI ACTGG 1 cut(s) 1789
BseRI GAGGAG 1 cut(s) 475
BseXI GCAGC 8 cut(s) 250, 415, 693, 1141, 1237, 1750, 1810, 1813
BseYI CCCAGC 2 cut(s) 1008, 1262
BshFI GGCC 4 cut(s) 257, 301, 936, 1039
BshNI GGYRCC 1 cut(s) 1345
BsiHKCI CYCGRG 1 cut(s) 571
BsiSI CCGG 2 cut(s) 329, 348
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 4 cut(s) 308, 524, 1015, 1713
BsmAI GTCTC 3 cut(s) 574, 768, 980
BsmFI GGGAC 1 cut(s) 674
BsmI GAATGC 2 cut(s) 1227, 1266
BsnI GGCC 4 cut(s) 257, 301, 936, 1039
BsoBI CYCGRG 1 cut(s) 571
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 1191
Bsp13I TCCGGA 1 cut(s) 347
Bsp143I GATC 4 cut(s) 575, 1576, 1664, 1762
BspACI CCGC 6 cut(s) 293, 309, 356, 709, 1343, 1601
BspANI GGCC 4 cut(s) 257, 301, 936, 1039
BspCNI CTCAG 4 cut(s) 30, 424, 562, 573
BspEI TCCGGA 1 cut(s) 347
BspLI GGNNCC 2 cut(s) 1188, 1347
BspMAI CTGCAG 2 cut(s) 268, 1740
BspMI ACCTGC 2 cut(s) 661, 1743
BspOI GCTAGC 1 cut(s) 837
BspPI GGATC 1 cut(s) 583
BspT104I TTCGAA 1 cut(s) 221
BspT107I GGYRCC 1 cut(s) 1345
BspTI CTTAAG 2 cut(s) 125, 1708
BsrDI GCAATG 1 cut(s) 894
BsrI ACTGG 1 cut(s) 1789
BssECI CCNNGG 6 cut(s) 296, 524, 937, 1349, 1419, 1811
BssMI GATC 4 cut(s) 575, 1576, 1664, 1762
BssNI GRCGYC 1 cut(s) 1346
BssT1I CCWWGG 4 cut(s) 937, 1349, 1419, 1811
Bst2UI CCWGG 2 cut(s) 525, 1476
Bst4CI ACNGT 4 cut(s) 13, 1136, 1716, 1877
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 1346
BstAFI CTTAAG 2 cut(s) 125, 1708
BstAPI GCANNNNNTGC 1 cut(s) 856
BstBAI YACGTR 2 cut(s) 1589, 1720
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 4 cut(s) 835, 1146, 1512, 1849
BstDEI CTNAG 9 cut(s) 17, 94, 213, 432, 549, 560, 721, 1552, 1658
BstF5I GGATG 4 cut(s) 337, 765, 1112, 1168
BstH2I RGCGCY 1 cut(s) 1349
BstHHI GCGC 1 cut(s) 1348
BstKTI GATC 4 cut(s) 578, 1579, 1667, 1765
BstMAI GTCTC 3 cut(s) 574, 768, 980
BstMBI GATC 4 cut(s) 575, 1576, 1664, 1762
BstMWI GCNNNNNNNGC 4 cut(s) 27, 263, 856, 1154
BstNI CCWGG 2 cut(s) 525, 1476
BstNSI RCATGY 2 cut(s) 854, 1307
BstPAI GACNNNNGTC 1 cut(s) 1625
BstSCI CCNGG 2 cut(s) 523, 1474
BstSFI CTRYAG 3 cut(s) 264, 1071, 1736
BstSNI TACGTA 1 cut(s) 1589
BstV1I GCAGC 8 cut(s) 250, 415, 693, 1141, 1237, 1750, 1810, 1813
BstV2I GAAGAC 1 cut(s) 1271
Bsu36I CCTNAGG 1 cut(s) 549
BsuI GTATCC 1 cut(s) 1452
BsuRI GGCC 4 cut(s) 257, 301, 936, 1039
BtgZI GCGATG 1 cut(s) 1828
BtrI CACGTC 1 cut(s) 900
BtsCI GGATG 4 cut(s) 337, 765, 1112, 1168
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 3 cut(s) 9, 420, 486
BveI ACCTGC 2 cut(s) 661, 1743
Cac8I GCNNGC 4 cut(s) 835, 1146, 1512, 1849
CaiI CAGNNNCTG 1 cut(s) 926
CfoI GCGC 1 cut(s) 1348
CseI GACGC 1 cut(s) 645
Csp6I GTAC 5 cut(s) 674, 1129, 1357, 1717, 1782
CviAII CATG 4 cut(s) 667, 851, 1304, 1852
CviQI GTAC 5 cut(s) 674, 1129, 1357, 1717, 1782
DdeI CTNAG 9 cut(s) 17, 94, 213, 432, 549, 560, 721, 1552, 1658
DinI GGCGCC 1 cut(s) 1347
DpnI GATC 4 cut(s) 577, 1578, 1666, 1764
DpnII GATC 4 cut(s) 575, 1576, 1664, 1762
DraIII CACNNNGTG 1 cut(s) 1083
EaeI YGGCCR 2 cut(s) 934, 1037
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EciI GGCGGA 1 cut(s) 282
Eco105I TACGTA 1 cut(s) 1589
Eco130I CCWWGG 4 cut(s) 937, 1349, 1419, 1811
Eco24I GRGCYC 1 cut(s) 1191
Eco57I CTGAAG 3 cut(s) 1109, 1122, 1200
Eco81I CCTNAGG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 571
EcoRI GAATTC 1 cut(s) 1727
EcoRII CCWGG 2 cut(s) 523, 1474
EcoT14I CCWWGG 4 cut(s) 937, 1349, 1419, 1811
EcoT38I GRGCYC 1 cut(s) 1191
EgeI GGCGCC 1 cut(s) 1347
EheI GGCGCC 1 cut(s) 1347
ErhI CCWWGG 4 cut(s) 937, 1349, 1419, 1811
FaeI CATG 4 cut(s) 670, 854, 1307, 1855
FalI AAGNNNNNCTT 2 cut(s) 163, 195
FaqI GGGAC 1 cut(s) 674
FatI CATG 4 cut(s) 666, 850, 1303, 1851
FauI CCCGC 2 cut(s) 302, 1594
FbaI TGATCA 1 cut(s) 1576
Fnu4HI GCNGC 9 cut(s) 264, 429, 707, 1155, 1226, 1344, 1739, 1799, 1802
FokI GGATG 4 cut(s) 344, 772, 1119, 1175
FriOI GRGCYC 1 cut(s) 1191
Fsp4HI GCNGC 9 cut(s) 264, 429, 707, 1155, 1226, 1344, 1739, 1799, 1802
FspBI CTAG 2 cut(s) 698, 834
GlaI GCGC 1 cut(s) 1347
GluI GCNGC 9 cut(s) 264, 429, 707, 1155, 1226, 1344, 1739, 1799, 1802
GsaI CCCAGC 2 cut(s) 1012, 1266
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 1 cut(s) 1349
HaeIII GGCC 4 cut(s) 257, 301, 936, 1039
HapII CCGG 2 cut(s) 329, 348
HgaI GACGC 1 cut(s) 645
HhaI GCGC 1 cut(s) 1348
Hin1I GRCGYC 1 cut(s) 1346
Hin1II CATG 4 cut(s) 670, 854, 1307, 1855
Hin6I GCGC 1 cut(s) 1346
HinP1I GCGC 1 cut(s) 1346
HincII GTYRAC 2 cut(s) 1005, 1620
HindII GTYRAC 2 cut(s) 1005, 1620
HindIII AAGCTT 2 cut(s) 1121, 1142
HpaII CCGG 2 cut(s) 329, 348
HphI GGTGA 1 cut(s) 1161
Hpy166II GTNNAC 3 cut(s) 1005, 1357, 1620
Hpy188I TCNGA 4 cut(s) 70, 459, 792, 1822
Hpy188III TCNNGA 9 cut(s) 189, 281, 348, 392, 551, 573, 731, 927, 1017
Hpy8I GTNNAC 3 cut(s) 1005, 1357, 1620
Hpy99I CGWCG 1 cut(s) 661
HpyAV CCTTC 4 cut(s) 147, 531, 710, 1097
HpyCH4III ACNGT 4 cut(s) 13, 1136, 1716, 1877
HpyCH4IV ACGT 4 cut(s) 899, 1522, 1588, 1719
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 263, 856, 1154
HpyF3I CTNAG 9 cut(s) 17, 94, 213, 432, 549, 560, 721, 1552, 1658
HpySE526I ACGT 4 cut(s) 899, 1522, 1588, 1719
Hsp92I GRCGYC 1 cut(s) 1346
Hsp92II CATG 4 cut(s) 670, 854, 1307, 1855
HspAI GCGC 1 cut(s) 1346
KasI GGCGCC 1 cut(s) 1345
Kpn2I TCCGGA 1 cut(s) 347
Ksp22I TGATCA 1 cut(s) 1576
Kzo9I GATC 4 cut(s) 575, 1576, 1664, 1762
LmnI GCTCC 4 cut(s) 295, 312, 1186, 1300
Lsp1109I GCAGC 8 cut(s) 250, 415, 693, 1141, 1237, 1750, 1810, 1813
LweI GCATC 5 cut(s) 846, 1497, 1523, 1557, 1677
MaeI CTAG 2 cut(s) 698, 834
MaeII ACGT 4 cut(s) 899, 1522, 1588, 1719
MaeIII GTNAC 5 cut(s) 322, 472, 1075, 1096, 1589
MalI GATC 4 cut(s) 577, 1578, 1666, 1764
MboI GATC 4 cut(s) 575, 1576, 1664, 1762
MboII GAAGA 6 cut(s) 37, 748, 1126, 1271, 1323, 1674
MfeI CAATTG 1 cut(s) 777
MhlI GDGCHC 1 cut(s) 1191
MlsI TGGCCA 1 cut(s) 1039
MluCI AATT 9 cut(s) 56, 143, 157, 162, 227, 648, 777, 1425, 1727
MluNI TGGCCA 1 cut(s) 1039
Mly113I GGCGCC 1 cut(s) 1346
MlyI GAGTC 4 cut(s) 338, 443, 1160, 1833
Mox20I TGGCCA 1 cut(s) 1039
MroI TCCGGA 1 cut(s) 347
MroXI GAANNNNTTC 2 cut(s) 227, 1748
MscI TGGCCA 1 cut(s) 1039
MslI CAYNNNNRTG 3 cut(s) 145, 203, 1505
Msp20I TGGCCA 1 cut(s) 1039
MspA1I CMGCKG 1 cut(s) 263
MspCI CTTAAG 2 cut(s) 125, 1708
MspI CCGG 2 cut(s) 329, 348
MspR9I CCNGG 2 cut(s) 525, 1476
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 2 cut(s) 1227, 1266
MvaI CCWGG 2 cut(s) 525, 1476
MwoI GCNNNNNNNGC 4 cut(s) 27, 263, 856, 1154
NarI GGCGCC 1 cut(s) 1346
NdeII GATC 4 cut(s) 575, 1576, 1664, 1762
NheI GCTAGC 1 cut(s) 833
NlaIII CATG 4 cut(s) 670, 854, 1307, 1855
NlaIV GGNNCC 2 cut(s) 1188, 1347
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 2 cut(s) 472, 1075
NspI RCATGY 2 cut(s) 854, 1307
NspV TTCGAA 1 cut(s) 221
OliI CACNNNNGTG 1 cut(s) 203
PctI GAATGC 2 cut(s) 1227, 1266
PdmI GAANNNNTTC 2 cut(s) 227, 1748
PfeI GAWTC 6 cut(s) 269, 454, 636, 923, 930, 1328
PkrI GCNGC 9 cut(s) 265, 430, 708, 1156, 1227, 1345, 1740, 1800, 1803
PleI GAGTC 4 cut(s) 338, 442, 1160, 1833
PluTI GGCGCC 1 cut(s) 1349
PpsI GAGTC 4 cut(s) 338, 442, 1160, 1833
Ppu21I YACGTR 2 cut(s) 1589, 1720
PshAI GACNNNNGTC 1 cut(s) 1625
PshBI ATTAAT 2 cut(s) 420, 1209
Psp6I CCWGG 2 cut(s) 523, 1474
PspFI CCCAGC 2 cut(s) 1008, 1262
PspGI CCWGG 2 cut(s) 523, 1474
PspN4I GGNNCC 2 cut(s) 1188, 1347
PstI CTGCAG 2 cut(s) 268, 1740
PstNI CAGNNNCTG 1 cut(s) 926
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 5 cut(s) 675, 1130, 1358, 1718, 1783
RsaNI GTAC 5 cut(s) 674, 1129, 1357, 1717, 1782
RseI CAYNNNNRTG 3 cut(s) 145, 203, 1505
SatI GCNGC 9 cut(s) 264, 429, 707, 1155, 1226, 1344, 1739, 1799, 1802
Sau3AI GATC 4 cut(s) 575, 1576, 1664, 1762
ScaI AGTACT 1 cut(s) 1130
SchI GAGTC 4 cut(s) 338, 443, 1160, 1833
ScrFI CCNGG 2 cut(s) 525, 1476
SduI GDGCHC 1 cut(s) 1191
SfaNI GCATC 5 cut(s) 846, 1497, 1523, 1557, 1677
SfcI CTRYAG 3 cut(s) 264, 1071, 1736
SfoI GGCGCC 1 cut(s) 1347
SfuI TTCGAA 1 cut(s) 221
SmiMI CAYNNNNRTG 3 cut(s) 145, 203, 1505
SmlI CTYRAG 2 cut(s) 125, 1708
SmoI CTYRAG 2 cut(s) 125, 1708
SnaBI TACGTA 1 cut(s) 1589
Sse9I AATT 9 cut(s) 56, 143, 157, 162, 227, 648, 777, 1425, 1727
SsiI CCGC 6 cut(s) 293, 309, 356, 709, 1343, 1601
SspDI GGCGCC 1 cut(s) 1345
SspI AATATT 1 cut(s) 969
SspMI CTAG 2 cut(s) 698, 834
StyD4I CCNGG 2 cut(s) 523, 1474
StyI CCWWGG 4 cut(s) 937, 1349, 1419, 1811
TaaI ACNGT 4 cut(s) 13, 1136, 1716, 1877
TaiI ACGT 4 cut(s) 902, 1525, 1591, 1722
TaqI TCGA 6 cut(s) 61, 221, 659, 1517, 1646, 1731
TasI AATT 9 cut(s) 56, 143, 157, 162, 227, 648, 777, 1425, 1727
TatI WGTACW 3 cut(s) 1128, 1356, 1781
TauI GCSGC 1 cut(s) 1346
TfiI GAWTC 6 cut(s) 269, 454, 636, 923, 930, 1328
TscAI CASTG 3 cut(s) 16, 420, 486
TseFI GTSAC 2 cut(s) 472, 1075
TseI GCWGC 8 cut(s) 263, 428, 706, 1154, 1225, 1738, 1798, 1801
Tsp45I GTSAC 2 cut(s) 472, 1075
TspDTI ATGAA 4 cut(s) 252, 502, 933, 1884
TspGWI ACGGA 1 cut(s) 1876
TspRI CASTG 3 cut(s) 16, 420, 486
Vha464I CTTAAG 2 cut(s) 125, 1708
VspI ATTAAT 2 cut(s) 420, 1209
XapI RAATTY 3 cut(s) 56, 157, 1727
XceI RCATGY 2 cut(s) 854, 1307
XmnI GAANNNNTTC 2 cut(s) 227, 1748
XspI CTAG 2 cut(s) 698, 834
ZrmI AGTACT 1 cut(s) 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.