RLG00000012141

Belongs to the SecY SEC61-alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008295
Physical Location & Seq
Reverse (-)
15689689 .. 15697058
7370 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012141

Sequence Viewer

Length: 2511 bp
ATGGAAGCCACTCTTCTCAGCTCTCAACGCTTCCACCCCCAACTCTTCCCCTCTAAATTTCGAGGTTCTGATACTCCAATTTGTTACCCACCCTTAGTTAGAACCAAAGCCAACTCTGTTTCCCTTAAGACCAGTCCTTCACAATTATGGATCACAAATTCAATTTTGCCAAGCAGGTCTTTATTCTCCAGAGTAAGCACAAGAGTGTCTGCTAAGTTTTCGAACCAATTCCGAAATGAATATACCGATGTTGAGGCCACAGCTGCAGAATCTCTAAATCTTGAAGATGCTCCACCGAGGCCGAATGGTGGGAGAGGTGTTGTTAAGCCGGATGAGATTGGCGACTCCGGTGGTGCGGAGGCCAAACCTGTAATGTTTAGGAACAGGTTTCTGGATTTTGTGAGGATGAGTTCAGTGATTAATAATGCTGCTGAGTCGTTTTTCAAAAGTGAGATTCGGAGGAGGTTGTTTGTGACGGCAGTGCTGATTGTGATTAGTCGTGTTGGTTATTTCATTCCTCTGCCTGGGTTTGATAGAAGGTTGATACCTCAGGATTATCTCAGCTTTGTCTCGGGATCAGTTGATGAGCTCGGCGATTTTACAGCAGAGCTTAAAATGTCCTTGTTCCAGCTTGGAATCAGTCCTCAAATTATAGCATCGATTATCATGCAGGTTCTCTGTTATGTCGTCCCCTCCCTAGTAAAGCTGCGGAAAGAAGGCTTAGATGGCCAAGAGAAGATTAAGAGTTATATATGGTGGATGTCTCTTGGCTTTGCAATTGTGGAGGCTGTGATACTTGCTTGTTATTCACTTCCCTATTCAATCTATGTAGCTAGCCACAGGGTCAAGCATGTGATGCTGACAAGTATGTTATTGGTCTGTGGTGCAATGACAATGACGTGGATATGTGATACCATTACAGAATCTGGATTCGGCCAAGGTTCATCTTTAATAATATGTGTGCAAATATTGATTGGCTACACAGAGACATTATACAAAATGTTGACCCAGCTTTCAGGCAGTTCTGTTAGTTGGTGGCCATACTTATTTGGAGTATTGGGCGTTTTCACTATTGTCACTATGTGGGCAGTTGTGGTAACTGAAGGATGTAGGAAGATAAAGCTTCAGTACTACGGTTTTAAGCTGGCTTCTGCTGCAAGGGATGACTCACCTATTACTGAAGTGGAGCCCTATATACCTTTTACTATTAATCCAGCAGGAATGCAGCCTGTTCTCACAACTACTTATCTCTTGGCATTTCCCAGCATTCTTGATGCTTATTTATCTGTCATGATGTTTGTCTCCTGCTGTGTTCATCAAATTTTTTATGCTTTTTTATGCTGTGCCAACTTGCCCAAGGAAATTGCTGACTACTTAAATAAGATGGGTGCAAGGATACCAAATATAAAACCTGGAAAGGCTACGATTGAATATCTCACAAAGATTCAGGCATCGACACGTTTTTGGGGTGGTGTATTGTTAAGCATCTTAGCAACAACATCAAGCATACTTGATCATTATTTACGTAACATCAATGCGGGATTTTCAATCGGGTTGACATCAGTCTTAATAATACTTCCAGGCAATCTTGTGCCTTCTAGTCCTAAGTGCTGGAATTCTCAGACCGATGAAACACCTGCAGCAGAAAGGGTTCTTACATTACGATCTGGTTTAAGGGATAGTTCTAGGTCTGTGCCTCTCGGGACGAAGAAGATAGAGTCATCGGAGATGGAGTTCACGGCCAACTCACTGGACGGCTCCGTCATCTTCCACGCCATTAACGAAATCTCAAGTTTCCTACTCTTCATGCACCAGCAAATCCCTGCTGTCTTGCAGGACATCACCACGGAATTCGATTCCCGCTGCGACGAGTACAAGGCCTTGGAATCGGCTCTGAGAGAAGACCAGGTGAAGCCGGCGCTGCGGAGAAAGCACGCCGGCGAAATGAGGGAAGCCAAGCGAGGGATTCGAAGGTTCCAGAAGCTGATGCACGGCGTTTCGAATTTCCAAACGGCGCTGAAGACGATCATCAGCGAAACTCCTCGGTTCCGAACAGCGCTTCTGATTCTCGGAGGGAATCCAATGCGGCCTCAACACGTCTATGAATTGAGCTTCTCCGGAAATGTCAGTGGCGAAGAATTGGATTTTGCGAAAAGCAGAGCGGCGGAAGTGCTTTGTAGAAAAGCGATTCGGGGGTTGGTCTCGGAGAATGTTGGGTCTGTTTCGTATCCGGGTCCTTGGAAGCTGTTCCTGTTGGTTAAAGCTCCCTCTTCTTTCAATCAGCCTCTGCATTTTCTTCCCAAGCGAGATTTTAAATACAGCAACAAGATTTTGCCTATGAGGCTAAGCTTCAAATGCAAAAACCAGAACCAGAACATTGATCAGTTTTCGCAAACCGGCAGCTCTGATGGGTTGGTTCAGTCTGCACCGGATGGTGACGATTTTATATGGTTTCAATGTCGACATGTAATCAAGGGAATAGCATTTAACACAGTACCTGAAGGAGAATAG

Protein Analysis

837

Amino Acids

93.23

Weight (kDa)

9.0

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecY PF00344 207 - 525 3e-61 SecY
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1645
AasI GACNNNNNNGTC 1 cut(s) 1760
Acc36I ACCTGC 3 cut(s) 165, 661, 1645
AccBSI CCGCTC 1 cut(s) 2162
AccI GTMKAC 1 cut(s) 2461
AccIII TCCGGA 1 cut(s) 2117
AciI CCGC 8 cut(s) 356, 709, 1538, 1861, 1924, 2086, 2162, 2165
AclWI GGATC 2 cut(s) 158, 583
AcoI YGGCCR 4 cut(s) 727, 934, 1037, 1740
AcsI RAATTY 6 cut(s) 56, 157, 1320, 1615, 1850, 2002
AcuI CTGAAG 4 cut(s) 1109, 1122, 1200, 2039
AdeI CACNNNGTG 1 cut(s) 1083
AfaI GTAC 3 cut(s) 1130, 1874, 2496
AfeI AGCGCT 1 cut(s) 2058
AfiI CCNNNNNNNGG 3 cut(s) 308, 524, 1962
AflII CTTAAG 1 cut(s) 125
AflIII ACRYGT 3 cut(s) 1457, 2095, 2464
AgsI TTSAA 9 cut(s) 162, 284, 445, 822, 1430, 1548, 2278, 2353, 2456
AjiI CACGTC 2 cut(s) 900, 2098
AjnI CCWGG 4 cut(s) 523, 1411, 1579, 1905
AleI CACNNNNGTG 1 cut(s) 203
AloI GAACNNNNNNTCC 2 cut(s) 1718, 1750
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 5 cut(s) 574, 768, 980, 1306, 2206
AlwI GGATC 2 cut(s) 158, 583
AlwNI CAGNNNCTG 4 cut(s) 926, 1984, 2287, 2498
Ama87I CYCGRG 2 cut(s) 571, 1700
Aor13HI TCCGGA 1 cut(s) 2117
Aor51HI AGCGCT 1 cut(s) 2058
AoxI GGCC 9 cut(s) 255, 299, 360, 727, 934, 1037, 1740, 1878, 2087
ApeKI GCWGC 9 cut(s) 263, 428, 706, 1154, 1225, 1640, 1863, 1921, 2400
ApoI RAATTY 6 cut(s) 56, 157, 1320, 1615, 1850, 2002
AseI ATTAAT 2 cut(s) 420, 1209
Asp700I GAANNNNTTC 3 cut(s) 227, 1650, 2246
AspLEI GCGC 3 cut(s) 1921, 2017, 2059
AspS9I GGNCC 1 cut(s) 2234
AsuC2I CCSGG 1 cut(s) 2232
AsuHPI GGTGA 4 cut(s) 1161, 1834, 1921, 2447
AsuII TTCGAA 3 cut(s) 221, 1969, 2000
AsuNHI GCTAGC 1 cut(s) 833
AvaI CYCGRG 2 cut(s) 571, 1700
AvaII GGWCC 1 cut(s) 2234
AxyI CCTNAGG 1 cut(s) 549
BalI TGGCCA 2 cut(s) 729, 1039
BanII GRGCYC 2 cut(s) 591, 1191
BbsI GAAGAC 2 cut(s) 1908, 2027
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 9 cut(s) 250, 415, 693, 1141, 1237, 1652, 1850, 1908, 2412
BccI CCATC 5 cut(s) 719, 1378, 1723, 2402, 2426
BceAI ACGGC 5 cut(s) 492, 1755, 1771, 2008, 2028
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 4 cut(s) 525, 1413, 1581, 1907
BciVI GTATCC 2 cut(s) 1389, 2238
BclI TGATCA 2 cut(s) 1513, 2380
BcnI CCSGG 1 cut(s) 2232
BcoDI GTCTC 5 cut(s) 574, 768, 980, 1306, 2206
BfaI CTAG 4 cut(s) 698, 834, 1599, 1686
BfmI CTRYAG 2 cut(s) 264, 1638
BfoI RGCGCY 3 cut(s) 1922, 2018, 2060
BfrI CTTAAG 1 cut(s) 125
BfuAI ACCTGC 3 cut(s) 165, 661, 1645
BfuI GTATCC 2 cut(s) 1389, 2238
BglI GCCNNNNNGGC 1 cut(s) 2341
BlpI GCTNAGC 1 cut(s) 2345
BmcAI AGTACT 1 cut(s) 1130
Bme1390I CCNGG 5 cut(s) 525, 1413, 1581, 1907, 2232
Bme18I GGWCC 1 cut(s) 2234
BmeT110I CYCGRG 2 cut(s) 571, 1700
BmgBI CACGTC 2 cut(s) 900, 2098
BmgT120I GGNCC 1 cut(s) 2234
BmiI GGNNCC 5 cut(s) 1188, 1759, 1976, 2048, 2235
BmrFI CCNGG 5 cut(s) 525, 1413, 1581, 1907, 2232
BmsI GCATC 7 cut(s) 277, 665, 846, 1264, 1460, 1494, 1977
BmtI GCTAGC 1 cut(s) 837
BoxI GACNNNNGTC 1 cut(s) 1562
BpiI GAAGAC 2 cut(s) 1908, 2027
BpmI CTGGAG 1 cut(s) 172
Bpu1102I GCTNAGC 1 cut(s) 2345
Bpu14I TTCGAA 3 cut(s) 221, 1969, 2000
BpuEI CTTGAG 1 cut(s) 1774
BpuMI CCSGG 1 cut(s) 2232
Bsa29I ATCGAT 1 cut(s) 659
BsaAI YACGTR 1 cut(s) 1526
BsaI GGTCTC 1 cut(s) 2206
BsaJI CCNNGG 8 cut(s) 296, 524, 937, 1356, 1845, 1881, 2042, 2237
BsaWI WCCGGW 3 cut(s) 347, 2117, 2428
BsaXI ACNNNNNCTCC 2 cut(s) 1718, 1748
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 524, 1962
Bse118I RCCGGY 3 cut(s) 1915, 1937, 2396
Bse1I ACTGG 2 cut(s) 132, 1755
Bse21I CCTNAGG 1 cut(s) 549
Bse3DI GCAATG 1 cut(s) 894
BseAI TCCGGA 1 cut(s) 2117
BseBI CCWGG 4 cut(s) 525, 1413, 1581, 1907
BseCI ATCGAT 1 cut(s) 659
BseDI CCNNGG 8 cut(s) 296, 524, 937, 1356, 1845, 1881, 2042, 2237
BseGI GGATG 6 cut(s) 337, 411, 765, 1112, 1168, 2437
BseLI CCNNNNNNNGG 3 cut(s) 308, 524, 1962
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 6 cut(s) 31, 423, 563, 574, 1634, 1886
BseNI ACTGG 2 cut(s) 132, 1755
BseRI GAGGAG 2 cut(s) 475, 2031
BseXI GCAGC 9 cut(s) 250, 415, 693, 1141, 1237, 1652, 1850, 1908, 2412
BseYI CCCAGC 2 cut(s) 1008, 1262
BsgI GTGCAG 1 cut(s) 2409
BshFI GGCC 9 cut(s) 257, 301, 362, 729, 936, 1039, 1742, 1880, 2089
BshVI ATCGAT 1 cut(s) 659
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 2 cut(s) 571, 1700
BsiSI CCGG 8 cut(s) 329, 348, 1916, 1938, 2118, 2231, 2397, 2429
BslFI GGGAC 2 cut(s) 674, 1717
BslI CCNNNNNNNGG 3 cut(s) 308, 524, 1962
BsmAI GTCTC 5 cut(s) 574, 768, 980, 1306, 2206
BsmFI GGGAC 2 cut(s) 674, 1717
BsmI GAATGC 2 cut(s) 1227, 1266
BsnI GGCC 9 cut(s) 257, 301, 362, 729, 936, 1039, 1742, 1880, 2089
Bso31I GGTCTC 1 cut(s) 2206
BsoBI CYCGRG 2 cut(s) 571, 1700
Bsp119I TTCGAA 3 cut(s) 221, 1969, 2000
Bsp1286I GDGCHC 2 cut(s) 591, 1191
Bsp13I TCCGGA 1 cut(s) 2117
Bsp143I GATC 6 cut(s) 150, 575, 1513, 1664, 2025, 2380
Bsp1720I GCTNAGC 1 cut(s) 2345
BspACI CCGC 8 cut(s) 356, 709, 1538, 1861, 1924, 2086, 2162, 2165
BspANI GGCC 9 cut(s) 257, 301, 362, 729, 936, 1039, 1742, 1880, 2089
BspCNI CTCAG 6 cut(s) 30, 424, 562, 573, 1633, 1887
BspDI ATCGAT 1 cut(s) 659
BspEI TCCGGA 1 cut(s) 2117
BspHI TCATGA 1 cut(s) 1290
BspLI GGNNCC 5 cut(s) 1188, 1759, 1976, 2048, 2235
BspMAI CTGCAG 2 cut(s) 268, 1642
BspMI ACCTGC 3 cut(s) 165, 661, 1645
BspOI GCTAGC 1 cut(s) 837
BspPI GGATC 2 cut(s) 158, 583
BspT104I TTCGAA 3 cut(s) 221, 1969, 2000
BspTI CTTAAG 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 2206
BsrBI CCGCTC 1 cut(s) 2162
BsrDI GCAATG 1 cut(s) 894
BsrFI RCCGGY 3 cut(s) 1915, 1937, 2396
BsrI ACTGG 2 cut(s) 132, 1755
BssAI RCCGGY 3 cut(s) 1915, 1937, 2396
BssECI CCNNGG 8 cut(s) 296, 524, 937, 1356, 1845, 1881, 2042, 2237
BssMI GATC 6 cut(s) 150, 575, 1513, 1664, 2025, 2380
BssT1I CCWWGG 4 cut(s) 937, 1356, 1881, 2237
Bst2UI CCWGG 4 cut(s) 525, 1413, 1581, 1907
Bst4CI ACNGT 2 cut(s) 1136, 2494
Bst6I CTCTTC 4 cut(s) 18, 50, 1808, 2275
BstAFI CTTAAG 1 cut(s) 125
BstAPI GCANNNNNTGC 1 cut(s) 856
BstBAI YACGTR 1 cut(s) 1526
BstBI TTCGAA 3 cut(s) 221, 1969, 2000
BstC8I GCNNGC 5 cut(s) 835, 1146, 1917, 1935, 1939
BstDSI CCRYGG 1 cut(s) 1845
BstF5I GGATG 6 cut(s) 337, 411, 765, 1112, 1168, 2437
BstH2I RGCGCY 3 cut(s) 1922, 2018, 2060
BstHHI GCGC 3 cut(s) 1921, 2017, 2059
BstKTI GATC 6 cut(s) 153, 578, 1516, 1667, 2028, 2383
BstMAI GTCTC 5 cut(s) 574, 768, 980, 1306, 2206
BstMBI GATC 6 cut(s) 150, 575, 1513, 1664, 2025, 2380
BstMWI GCNNNNNNNGC 9 cut(s) 27, 263, 726, 856, 1154, 1921, 1930, 2341, 2355
BstNI CCWGG 4 cut(s) 525, 1413, 1581, 1907
BstNSI RCATGY 2 cut(s) 854, 2468
BstPAI GACNNNNGTC 1 cut(s) 1562
BstSCI CCNGG 5 cut(s) 523, 1411, 1579, 1905, 2230
BstSFI CTRYAG 2 cut(s) 264, 1638
BstSNI TACGTA 1 cut(s) 1526
BstV1I GCAGC 9 cut(s) 250, 415, 693, 1141, 1237, 1652, 1850, 1908, 2412
BstV2I GAAGAC 2 cut(s) 1908, 2027
BstXI CCANNNNNNTGG 1 cut(s) 1750
Bsu15I ATCGAT 1 cut(s) 659
Bsu36I CCTNAGG 1 cut(s) 549
BsuI GTATCC 2 cut(s) 1389, 2238
BsuRI GGCC 9 cut(s) 257, 301, 362, 729, 936, 1039, 1742, 1880, 2089
BsuTUI ATCGAT 1 cut(s) 659
BtgI CCRYGG 1 cut(s) 1845
BtrI CACGTC 2 cut(s) 900, 2098
BtsCI GGATG 6 cut(s) 337, 411, 765, 1112, 1168, 2437
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 4 cut(s) 420, 486, 1748, 2134
BveI ACCTGC 3 cut(s) 165, 661, 1645
Cac8I GCNNGC 5 cut(s) 835, 1146, 1917, 1935, 1939
CaiI CAGNNNCTG 4 cut(s) 926, 1984, 2287, 2498
CciI TCATGA 1 cut(s) 1290
CfoI GCGC 3 cut(s) 1921, 2017, 2059
Cfr10I RCCGGY 3 cut(s) 1915, 1937, 2396
Cfr13I GGNCC 1 cut(s) 2234
ClaI ATCGAT 1 cut(s) 659
CsiI ACCWGGT 1 cut(s) 1905
Csp6I GTAC 3 cut(s) 1129, 1873, 2495
CviAII CATG 5 cut(s) 667, 851, 1291, 1807, 2465
CviQI GTAC 3 cut(s) 1129, 1873, 2495
DpnI GATC 6 cut(s) 152, 577, 1515, 1666, 2027, 2382
DpnII GATC 6 cut(s) 150, 575, 1513, 1664, 2025, 2380
DraI TTTAAA 1 cut(s) 2314
DraIII CACNNNGTG 1 cut(s) 1083
DrdI GACNNNNNNGTC 1 cut(s) 1760
DseDI GACNNNNNNGTC 1 cut(s) 1760
EaeI YGGCCR 4 cut(s) 727, 934, 1037, 1740
Eam1104I CTCTTC 4 cut(s) 18, 50, 1808, 2275
EarI CTCTTC 4 cut(s) 18, 50, 1808, 2275
EciI GGCGGA 1 cut(s) 2180
Ecl136II GAGCTC 1 cut(s) 589
Eco105I TACGTA 1 cut(s) 1526
Eco130I CCWWGG 4 cut(s) 937, 1356, 1881, 2237
Eco147I AGGCCT 1 cut(s) 1880
Eco24I GRGCYC 2 cut(s) 591, 1191
Eco31I GGTCTC 1 cut(s) 2206
Eco47I GGWCC 1 cut(s) 2234
Eco47III AGCGCT 1 cut(s) 2058
Eco53kI GAGCTC 1 cut(s) 589
Eco57I CTGAAG 4 cut(s) 1109, 1122, 1200, 2039
Eco81I CCTNAGG 1 cut(s) 549
Eco88I CYCGRG 2 cut(s) 571, 1700
EcoICRI GAGCTC 1 cut(s) 589
EcoO109I RGGNCCY 1 cut(s) 2234
EcoRI GAATTC 2 cut(s) 1615, 1850
EcoRII CCWGG 4 cut(s) 523, 1411, 1579, 1905
EcoT14I CCWWGG 4 cut(s) 937, 1356, 1881, 2237
EcoT38I GRGCYC 2 cut(s) 591, 1191
ErhI CCWWGG 4 cut(s) 937, 1356, 1881, 2237
FaeI CATG 5 cut(s) 670, 854, 1294, 1810, 2468
FalI AAGNNNNNCTT 3 cut(s) 29, 163, 195
FaqI GGGAC 2 cut(s) 674, 1717
FatI CATG 5 cut(s) 666, 850, 1290, 1806, 2464
FauI CCCGC 2 cut(s) 1531, 1868
FbaI TGATCA 2 cut(s) 1513, 2380
FblI GTMKAC 1 cut(s) 2461
FokI GGATG 6 cut(s) 344, 418, 772, 1119, 1175, 2444
FriOI GRGCYC 2 cut(s) 591, 1191
FspBI CTAG 4 cut(s) 698, 834, 1599, 1686
GlaI GCGC 3 cut(s) 1920, 2016, 2058
GsaI CCCAGC 2 cut(s) 1012, 1266
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 3 cut(s) 1922, 2018, 2060
HaeIII GGCC 9 cut(s) 257, 301, 362, 729, 936, 1039, 1742, 1880, 2089
HapII CCGG 8 cut(s) 329, 348, 1916, 1938, 2118, 2231, 2397, 2429
HhaI GCGC 3 cut(s) 1921, 2017, 2059
Hin1II CATG 5 cut(s) 670, 854, 1294, 1810, 2468
Hin6I GCGC 3 cut(s) 1919, 2015, 2057
HinP1I GCGC 3 cut(s) 1919, 2015, 2057
HincII GTYRAC 3 cut(s) 1005, 1557, 2462
HindII GTYRAC 3 cut(s) 1005, 1557, 2462
HindIII AAGCTT 2 cut(s) 1121, 2347
HpaII CCGG 8 cut(s) 329, 348, 1916, 1938, 2118, 2231, 2397, 2429
HphI GGTGA 4 cut(s) 1161, 1834, 1921, 2447
Hpy166II GTNNAC 4 cut(s) 1005, 1557, 1737, 2462
Hpy8I GTNNAC 4 cut(s) 1005, 1557, 1737, 2462
Hpy99I CGWCG 1 cut(s) 1871
HpyAV CCTTC 7 cut(s) 147, 531, 710, 1097, 1605, 1965, 2495
HpyCH4III ACNGT 2 cut(s) 1136, 2494
HpyCH4IV ACGT 4 cut(s) 899, 1459, 1525, 2097
HpyF10VI GCNNNNNNNGC 9 cut(s) 27, 263, 726, 856, 1154, 1921, 1930, 2341, 2355
HpySE526I ACGT 4 cut(s) 899, 1459, 1525, 2097
Hsp92II CATG 5 cut(s) 670, 854, 1294, 1810, 2468
HspAI GCGC 3 cut(s) 1919, 2015, 2057
Kpn2I TCCGGA 1 cut(s) 2117
KroI GCCGGC 2 cut(s) 1915, 1937
KroNI GCCGGC 2 cut(s) 1917, 1939
Ksp22I TGATCA 2 cut(s) 1513, 2380
Kzo9I GATC 6 cut(s) 150, 575, 1513, 1664, 2025, 2380
LmnI GCTCC 4 cut(s) 295, 1186, 1763, 2269
Lsp1109I GCAGC 9 cut(s) 250, 415, 693, 1141, 1237, 1652, 1850, 1908, 2412
LweI GCATC 7 cut(s) 277, 665, 846, 1264, 1460, 1494, 1977
MabI ACCWGGT 1 cut(s) 1905
MaeI CTAG 4 cut(s) 698, 834, 1599, 1686
MaeII ACGT 4 cut(s) 899, 1459, 1525, 2097
MaeIII GTNAC 6 cut(s) 83, 472, 1075, 1096, 1526, 2435
MalI GATC 6 cut(s) 152, 577, 1515, 1666, 2027, 2382
MbiI CCGCTC 1 cut(s) 2162
MboI GATC 6 cut(s) 150, 575, 1513, 1664, 2025, 2380
MfeI CAATTG 1 cut(s) 777
MhlI GDGCHC 2 cut(s) 591, 1191
MlsI TGGCCA 2 cut(s) 729, 1039
MluNI TGGCCA 2 cut(s) 729, 1039
MlyI GAGTC 4 cut(s) 338, 443, 1160, 1727
Mox20I TGGCCA 2 cut(s) 729, 1039
MreI CGCCGGCG 1 cut(s) 1937
MroI TCCGGA 1 cut(s) 2117
MroNI GCCGGC 2 cut(s) 1915, 1937
MroXI GAANNNNTTC 3 cut(s) 227, 1650, 2246
MscI TGGCCA 2 cut(s) 729, 1039
MslI CAYNNNNRTG 3 cut(s) 145, 203, 2100
Msp20I TGGCCA 2 cut(s) 729, 1039
MspA1I CMGCKG 2 cut(s) 263, 1863
MspCI CTTAAG 1 cut(s) 125
MspI CCGG 8 cut(s) 329, 348, 1916, 1938, 2118, 2231, 2397, 2429
MspR9I CCNGG 5 cut(s) 525, 1413, 1581, 1907, 2232
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 2 cut(s) 1227, 1266
MvaI CCWGG 4 cut(s) 525, 1413, 1581, 1907
MwoI GCNNNNNNNGC 9 cut(s) 27, 263, 726, 856, 1154, 1921, 1930, 2341, 2355
NaeI GCCGGC 2 cut(s) 1917, 1939
NciI CCSGG 1 cut(s) 2232
NdeII GATC 6 cut(s) 150, 575, 1513, 1664, 2025, 2380
NgoMIV GCCGGC 2 cut(s) 1915, 1937
NheI GCTAGC 1 cut(s) 833
NlaIII CATG 5 cut(s) 670, 854, 1294, 1810, 2468
NlaIV GGNNCC 5 cut(s) 1188, 1759, 1976, 2048, 2235
NmeAIII GCCGAG 1 cut(s) 570
NmuCI GTSAC 3 cut(s) 472, 1075, 2435
NspI RCATGY 2 cut(s) 854, 2468
NspV TTCGAA 3 cut(s) 221, 1969, 2000
OliI CACNNNNGTG 1 cut(s) 203
PagI TCATGA 1 cut(s) 1290
PaqCI CACCTGC 1 cut(s) 1645
PceI AGGCCT 1 cut(s) 1880
PciI ACATGT 1 cut(s) 2464
PcsI WCGNNNNNNNCGW 1 cut(s) 1779
PctI GAATGC 2 cut(s) 1227, 1266
PdiI GCCGGC 2 cut(s) 1917, 1939
PdmI GAANNNNTTC 3 cut(s) 227, 1650, 2246
PleI GAGTC 4 cut(s) 338, 442, 1160, 1726
PpsI GAGTC 4 cut(s) 338, 442, 1160, 1726
Ppu21I YACGTR 1 cut(s) 1526
PpuMI RGGWCCY 1 cut(s) 2234
PscI ACATGT 1 cut(s) 2464
PshAI GACNNNNGTC 1 cut(s) 1562
PshBI ATTAAT 2 cut(s) 420, 1209
Psp124BI GAGCTC 1 cut(s) 591
Psp5II RGGWCCY 1 cut(s) 2234
Psp6I CCWGG 4 cut(s) 523, 1411, 1579, 1905
PspFI CCCAGC 2 cut(s) 1008, 1262
PspGI CCWGG 4 cut(s) 523, 1411, 1579, 1905
PspN4I GGNNCC 5 cut(s) 1188, 1759, 1976, 2048, 2235
PspPI GGNCC 1 cut(s) 2234
PspPPI RGGWCCY 1 cut(s) 2234
PstI CTGCAG 2 cut(s) 268, 1642
PstNI CAGNNNCTG 4 cut(s) 926, 1984, 2287, 2498
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 3 cut(s) 1130, 1874, 2496
RsaNI GTAC 3 cut(s) 1129, 1873, 2495
RseI CAYNNNNRTG 3 cut(s) 145, 203, 2100
SacI GAGCTC 1 cut(s) 591
SalI GTCGAC 1 cut(s) 2460
Sau3AI GATC 6 cut(s) 150, 575, 1513, 1664, 2025, 2380
Sau96I GGNCC 1 cut(s) 2234
ScaI AGTACT 1 cut(s) 1130
SchI GAGTC 4 cut(s) 338, 443, 1160, 1727
ScrFI CCNGG 5 cut(s) 525, 1413, 1581, 1907, 2232
SduI GDGCHC 2 cut(s) 591, 1191
SexAI ACCWGGT 1 cut(s) 1905
SfaNI GCATC 7 cut(s) 277, 665, 846, 1264, 1460, 1494, 1977
SfcI CTRYAG 2 cut(s) 264, 1638
SfuI TTCGAA 3 cut(s) 221, 1969, 2000
SgrAI CRCCGGYG 1 cut(s) 1937
SinI GGWCC 1 cut(s) 2234
SmiMI CAYNNNNRTG 3 cut(s) 145, 203, 2100
SmlI CTYRAG 2 cut(s) 125, 1789
SmoI CTYRAG 2 cut(s) 125, 1789
SnaBI TACGTA 1 cut(s) 1526
SseBI AGGCCT 1 cut(s) 1880
SsiI CCGC 8 cut(s) 356, 709, 1538, 1861, 1924, 2086, 2162, 2165
SspI AATATT 1 cut(s) 969
SspMI CTAG 4 cut(s) 698, 834, 1599, 1686
SstI GAGCTC 1 cut(s) 591
StuI AGGCCT 1 cut(s) 1880
StyD4I CCNGG 5 cut(s) 523, 1411, 1579, 1905, 2230
StyI CCWWGG 4 cut(s) 937, 1356, 1881, 2237
TaaI ACNGT 2 cut(s) 1136, 2494
TaiI ACGT 4 cut(s) 902, 1462, 1528, 2100
TaqI TCGA 8 cut(s) 61, 221, 659, 1454, 1854, 1969, 2000, 2461
TaqII GACCGA 1 cut(s) 1640
TatI WGTACW 2 cut(s) 1128, 1872
TauI GCSGC 2 cut(s) 2089, 2165
TscAI CASTG 4 cut(s) 420, 486, 1755, 2134
TseFI GTSAC 3 cut(s) 472, 1075, 2435
TseI GCWGC 9 cut(s) 263, 428, 706, 1154, 1225, 1640, 1863, 1921, 2400
Tsp45I GTSAC 3 cut(s) 472, 1075, 2435
TspDTI ATGAA 7 cut(s) 252, 502, 933, 1304, 1644, 1795, 2118
TspGWI ACGGA 2 cut(s) 1750, 1862
TspRI CASTG 4 cut(s) 420, 486, 1755, 2134
Vha464I CTTAAG 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 2234
VspI ATTAAT 2 cut(s) 420, 1209
XapI RAATTY 6 cut(s) 56, 157, 1320, 1615, 1850, 2002
XceI RCATGY 2 cut(s) 854, 2468
XmiI GTMKAC 1 cut(s) 2461
XmnI GAANNNNTTC 3 cut(s) 227, 1650, 2246
XspI CTAG 4 cut(s) 698, 834, 1599, 1686
ZrmI AGTACT 1 cut(s) 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.