Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0008295

Physical Location & Seq

Genomic Coordinates Forward (+)

21572140 .. 21576799

Gene Structure 4660 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g21260.11

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1674 bp

ATGGAAGCCGCTCTTCTGAGCTCGCAACGCTTCCATCCCAAGCTTTCACCCGAAAATTCTCGAGGTGCAGTTTCAGGTAGTGAAATTCAAATTTGTTGTTTGCCCCTTGTTAGGAATAGCTATGTTTCACTTAGGACAAACCCTAGAAATTTCATGTTTCCAAACAGTCCCTTATTGTCCAGATTGAACAGAAGAGTCTCTGTTAAGTTCTCGAAACAGTTCGTGAATGATTATACAAATGTTGAGGCTACACCTCCAGAGTCTCTAAATGTTGAAGTTAATCCGTTGAGTGGGAGTGACAGGACGGAAATTTCAAAACAGAACGCGGTTGAGAATGCAAAGGCGGAGCAGGCGGGATCTATCACGTTTAGAAACCGGTTTCTGAATTTCGTGCGCCTGAGTTCAGTGGTTAATAATGCTGCTGAATCATTCTTCAAGAGCGAGATTAGGAGGAGGCTGTTTGTGACAGCAGTGCTAATTGTAATTAGTCGTGTCGGTTATTTCATTCCTCTGCCTGGATTTGATAGAAGGTTAATCCCTCAAGATTACCTAAGCTTTGTATCGGGATCTGTTGATGAACTCGGTGATTTTGCAGCAGAGCTTAAAATGTCTTTCTTTCAGCTTGGAATCAGTCCTCAAATCATAGCGTCAATCATCATGCAGGTACTCTGTCATGTTGTTCCCTCCCTGGTAAAGCTGCGGAAGGAAGGCTTAGATGGTCATGAGAAGATTAAGAGTTATATATGGTGGCTGTCTTTCGGCTTTGCAATAGTGGAAGCTATAATACTTGCTTGTTATTCACTTCCATATTCAATCTATGCGGCTAGCTACAGGGTCAAGCATGTGATGGTGACAAGTATGTTATTGGTTTGTGGAGCAATGGCAATGACGTGGATTTGTGATACCATCTCAGAATCTGGATTTGGTCAAGGTTCATCTCTAATTATTTGTGTACAAATATTGATTGGCTACACAGAGACATTATACAAAATGCTGTCTCAGCTCTCAGGAAGTTCTGTTAGTTGGGGCCCATATTTATTTGCAGTATTGGGCGTTTTCACTATAGTCACTATGTGGGCAGTTGTGGTGACTGAAGGGTGCAGGAAGATAAAGCTGCAGTACTATGGTTTTAAGCTTGCTTCTGCTGCAAGAGATGACTCACCTATTACCGAAGTGGAGCCCTATATCCCTTTTACCATTAACCCATCAGGAATGCAGCCTGTTCTCACCACTACTTATCTCTTGGCATTTCCCAGCATTCTTGCAAGTATTCTTGGTTCACCTTTCTGGGAGCATGTTAAGGAGATATTGAACCCCGAAAATACTATTGGTGCAGGGCCTTGGGTGTACTACTCAATATATGCGTTTTTTGTGTTCTTATTCAATATATTTGACATTGCCAACTTGCCCAAGGAAATTGCTGATTACTTGAATAAGATGGGTGCGAGGATACCAAACATAAAACCTGGGAAGGCTACAATTGAGTACCTTACAAAGATTCAGGCATCGACACGTTTCTGGGGGGGGCTATTGTTAAGTATCTTGGCAACAGCATCAAGCATAATCGACCATTATTTACGCAATATCAATGCGGGATTTGCAATTGGGTTGACATCAGTCCTAATAATAGTGGGTTCTATTATTGAACTTAGAACGATCATATCAGGCATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008104 GO:0008150 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009668 GO:0009987 GO:0010027 GO:0016043 GO:0031967 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051641 GO:0061024 GO:0070727 GO:0071840 GO:0072598

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SecY

Protein Analysis

558

Amino Acids

61.86

Weight (kDa)

8.92

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008295)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31530
fragaria_vesca	FvH4_2g23670 FvH4_2g23670 FvH4_2g23670 FvH4_2g23670 FvH4_2g23670 FvH4_2g23670
malus_domestica	MD03G1266100.v1.1 MD11G1286500.v1.1
prunus_persica	Prupe.8G241800_v2.0.a1
pyrus_communis	pycom03g21260 pycom11g25400
rosa_chinensis	RchiOBHm_Chr6g0291131
rosa_laevigata	RLG00000012141
rosa_multiflora	Rmu_sc0004642.1_g000022 Rmu_sc0013275.1_g000001
rosa_roxburghii	Rroxscaffold_7G00176350
rosa_rugosa	Rorug06G0217900
rosa_samantha	Rh6AG328200 Rh6BG335700 Rh6CG341800 Rh6DG329100
rosa_wichuraiana	Rw6G028550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	652
AccBSI	CCGCTC	1 cut(s)	11
AccII	CGCG	1 cut(s)	326
AciI	CCGC	7 cut(s)	9, 326, 344, 353, 700, 821, 1592
AclWI	GGATC	2 cut(s)	364, 574
AcsI	RAATTY	6 cut(s)	55, 84, 90, 148, 309, 385
AcuI	CTGAAG	1 cut(s)	1113
AdeI	CACNNNGTG	1 cut(s)	1074
AfaI	GTAC	5 cut(s)	666, 954, 1121, 1349, 1487
AfiI	CCNNNNNNNGG	3 cut(s)	111, 290, 515
AflIII	ACRYGT	1 cut(s)	1511
AgeI	ACCGGT	1 cut(s)	375
AjiI	CACGTC	1 cut(s)	891
AjnI	CCWGG	3 cut(s)	514, 687, 1465
AjuI	GAANNNNNNNTTGG	4 cut(s)	906, 938, 1311, 1343
Alw21I	GWGCWC	1 cut(s)	23
Alw26I	GTCTC	4 cut(s)	202, 267, 971, 1002
AlwI	GGATC	2 cut(s)	364, 574
AlwNI	CAGNNNCTG	1 cut(s)	917
Ama87I	CYCGRG	1 cut(s)	60
AoxI	GGCC	2 cut(s)	1027, 1337
ApaI	GGGCCC	1 cut(s)	1031
ApeKI	GCWGC	6 cut(s)	419, 593, 697, 1114, 1145, 1216
ApoI	RAATTY	6 cut(s)	55, 84, 90, 148, 309, 385
AsiGI	ACCGGT	1 cut(s)	375
Asp700I	GAANNNNTTC	1 cut(s)	218
AspLEI	GCGC	1 cut(s)	396
AspS9I	GGNCC	3 cut(s)	1027, 1028, 1337
AsuHPI	GGTGA	7 cut(s)	39, 596, 862, 1099, 1152, 1219, 1272
AsuNHI	GCTAGC	1 cut(s)	824
AvaI	CYCGRG	1 cut(s)	60
BaeGI	GKGCMC	1 cut(s)	1031
BanII	GRGCYC	3 cut(s)	23, 1031, 1182
BarI	GAAGNNNNNNTAC	2 cut(s)	768, 800
Bbv12I	GWGCWC	1 cut(s)	23
BbvI	GCAGC	6 cut(s)	406, 605, 684, 1101, 1132, 1228
BccI	CCATC	6 cut(s)	42, 710, 841, 914, 1213, 1432
BciT130I	CCWGG	3 cut(s)	516, 689, 1467
BciVI	GTATCC	1 cut(s)	1443
BcoDI	GTCTC	4 cut(s)	202, 267, 971, 1002
BfaI	CTAG	2 cut(s)	144, 825
BfmI	CTRYAG	3 cut(s)	829, 1062, 1115
BfuAI	ACCTGC	1 cut(s)	652
BfuI	GTATCC	1 cut(s)	1443
BisI	GCNGC	8 cut(s)	9, 420, 594, 698, 822, 1115, 1146, 1217
BlsI	GCNGC	8 cut(s)	10, 421, 595, 699, 823, 1116, 1147, 1218
BmcAI	AGTACT	1 cut(s)	1121
Bme1390I	CCNGG	3 cut(s)	516, 689, 1467
BmeT110I	CYCGRG	1 cut(s)	60
BmgBI	CACGTC	1 cut(s)	891
BmgT120I	GGNCC	3 cut(s)	1027, 1028, 1337
BmiI	GGNNCC	3 cut(s)	1028, 1029, 1179
BmrFI	CCNGG	3 cut(s)	516, 689, 1467
BmsI	GCATC	2 cut(s)	1514, 1562
BmtI	GCTAGC	1 cut(s)	828
BoxI	GACNNNNGTC	1 cut(s)	1616
BpmI	CTGGAG	1 cut(s)	240
Bpu10I	CCTNAGC	1 cut(s)	551
BpuEI	CTTGAG	1 cut(s)	525
BsaJI	CCNNGG	4 cut(s)	687, 1340, 1410, 1466
BsaWI	WCCGGW	1 cut(s)	375
Bsc4I	CCNNNNNNNGG	3 cut(s)	111, 290, 515
Bse118I	RCCGGY	1 cut(s)	375
Bse3DI	GCAATG	3 cut(s)	885, 891, 1395
BseBI	CCWGG	3 cut(s)	516, 689, 1467
BseDI	CCNNGG	4 cut(s)	687, 1340, 1410, 1466
BseGI	GGATG	1 cut(s)	34
BseLI	CCNNNNNNNGG	3 cut(s)	111, 290, 515
BseMI	GCAATG	3 cut(s)	885, 891, 1395
BseMII	CTCAG	5 cut(s)	8, 389, 924, 1013, 1020
BseRI	GAGGAG	1 cut(s)	466
BseSI	GKGCMC	1 cut(s)	1031
BseXI	GCAGC	6 cut(s)	406, 605, 684, 1101, 1132, 1228
BseYI	CCCAGC	1 cut(s)	1253
BsgI	GTGCAG	3 cut(s)	87, 1120, 1353
Bsh1236I	CGCG	1 cut(s)	326
BshFI	GGCC	2 cut(s)	1029, 1339
BshTI	ACCGGT	1 cut(s)	375
BsiHKAI	GWGCWC	1 cut(s)	23
BsiHKCI	CYCGRG	1 cut(s)	60
BsiSI	CCGG	1 cut(s)	376
BslFI	GGGAC	1 cut(s)	153
BslI	CCNNNNNNNGG	3 cut(s)	111, 290, 515
BsmAI	GTCTC	4 cut(s)	202, 267, 971, 1002
BsmFI	GGGAC	1 cut(s)	153
BsmI	GAATGC	3 cut(s)	340, 1218, 1257
BsnI	GGCC	2 cut(s)	1029, 1339
BsoBI	CYCGRG	1 cut(s)	60
Bsp120I	GGGCCC	1 cut(s)	1027
Bsp1286I	GDGCHC	3 cut(s)	23, 1031, 1182
Bsp1407I	TGTACA	1 cut(s)	952
Bsp143I	GATC	3 cut(s)	356, 566, 1656
BspACI	CCGC	7 cut(s)	9, 326, 344, 353, 700, 821, 1592
BspANI	GGCC	2 cut(s)	1029, 1339
BspCNI	CTCAG	5 cut(s)	9, 390, 923, 1012, 1019
BspFNI	CGCG	1 cut(s)	326
BspHI	TCATGA	1 cut(s)	721
BspLI	GGNNCC	3 cut(s)	1028, 1029, 1179
BspMAI	CTGCAG	1 cut(s)	1119
BspMI	ACCTGC	1 cut(s)	652
BspOI	GCTAGC	1 cut(s)	828
BspPI	GGATC	2 cut(s)	364, 574
BspQI	GCTCTTC	1 cut(s)	18
BsrBI	CCGCTC	1 cut(s)	11
BsrDI	GCAATG	3 cut(s)	885, 891, 1395
BsrFI	RCCGGY	1 cut(s)	375
BsrGI	TGTACA	1 cut(s)	952
BssAI	RCCGGY	1 cut(s)	375
BssECI	CCNNGG	4 cut(s)	687, 1340, 1410, 1466
BssMI	GATC	3 cut(s)	356, 566, 1656
BssT1I	CCWWGG	2 cut(s)	1340, 1410
Bst2UI	CCWGG	3 cut(s)	516, 689, 1467
Bst4CI	ACNGT	2 cut(s)	167, 219
Bst6I	CTCTTC	2 cut(s)	18, 187
BstAUI	TGTACA	1 cut(s)	952
BstC8I	GCNNGC	4 cut(s)	23, 351, 826, 1137
BstDEI	CTNAG	9 cut(s)	17, 131, 398, 551, 712, 910, 999, 1006, 1649
BstF5I	GGATG	1 cut(s)	34
BstFNI	CGCG	1 cut(s)	326
BstHHI	GCGC	1 cut(s)	396
BstKTI	GATC	3 cut(s)	359, 569, 1659
BstMAI	GTCTC	4 cut(s)	202, 267, 971, 1002
BstMBI	GATC	3 cut(s)	356, 566, 1656
BstMWI	GCNNNNNNNGC	5 cut(s)	27, 350, 1000, 1145, 1598
BstNI	CCWGG	3 cut(s)	516, 689, 1467
BstNSI	RCATGY	2 cut(s)	845, 1298
BstPAI	GACNNNNGTC	1 cut(s)	1616
BstSCI	CCNGG	3 cut(s)	514, 687, 1465
BstSFI	CTRYAG	3 cut(s)	829, 1062, 1115
BstSLI	GKGCMC	1 cut(s)	1031
BstUI	CGCG	1 cut(s)	326
BstV1I	GCAGC	6 cut(s)	406, 605, 684, 1101, 1132, 1228
BstX2I	RGATCY	2 cut(s)	356, 566
BstYI	RGATCY	2 cut(s)	356, 566
BsuI	GTATCC	1 cut(s)	1443
BsuRI	GGCC	2 cut(s)	1029, 1339
BtrI	CACGTC	1 cut(s)	891
BtsCI	GGATG	1 cut(s)	34
BtsI	GCAGTG	1 cut(s)	477
BtsIMutI	CAGTG	2 cut(s)	411, 477
BveI	ACCTGC	1 cut(s)	652
Cac8I	GCNNGC	4 cut(s)	23, 351, 826, 1137
CaiI	CAGNNNCTG	1 cut(s)	917
CciI	TCATGA	1 cut(s)	721
CfoI	GCGC	1 cut(s)	396
Cfr10I	RCCGGY	1 cut(s)	375
Cfr13I	GGNCC	3 cut(s)	1027, 1028, 1337
CseI	GACGC	1 cut(s)	636
Csp6I	GTAC	5 cut(s)	665, 953, 1120, 1348, 1486
CspAI	ACCGGT	1 cut(s)	375
CviAII	CATG	6 cut(s)	154, 658, 674, 722, 842, 1295
CviQI	GTAC	5 cut(s)	665, 953, 1120, 1348, 1486
DdeI	CTNAG	9 cut(s)	17, 131, 398, 551, 712, 910, 999, 1006, 1649
DpnI	GATC	3 cut(s)	358, 568, 1658
DpnII	GATC	3 cut(s)	356, 566, 1656
DraIII	CACNNNGTG	1 cut(s)	1074
Eam1104I	CTCTTC	2 cut(s)	18, 187
EarI	CTCTTC	2 cut(s)	18, 187
EciI	GGCGGA	1 cut(s)	359
Ecl136II	GAGCTC	1 cut(s)	21
Eco130I	CCWWGG	2 cut(s)	1340, 1410
Eco24I	GRGCYC	3 cut(s)	23, 1031, 1182
Eco53kI	GAGCTC	1 cut(s)	21
Eco57I	CTGAAG	1 cut(s)	1113
Eco88I	CYCGRG	1 cut(s)	60
EcoICRI	GAGCTC	1 cut(s)	21
EcoO109I	RGGNCCY	2 cut(s)	1027, 1337
EcoRII	CCWGG	3 cut(s)	514, 687, 1465
EcoT14I	CCWWGG	2 cut(s)	1340, 1410
EcoT38I	GRGCYC	3 cut(s)	23, 1031, 1182
ErhI	CCWWGG	2 cut(s)	1340, 1410
FaeI	CATG	6 cut(s)	157, 661, 677, 725, 845, 1298
FalI	AAGNNNNNCTT	3 cut(s)	29, 695, 727
FaqI	GGGAC	1 cut(s)	153
FatI	CATG	6 cut(s)	153, 657, 673, 721, 841, 1294
FauI	CCCGC	2 cut(s)	346, 1585
Fnu4HI	GCNGC	8 cut(s)	9, 420, 594, 698, 822, 1115, 1146, 1217
FokI	GGATG	1 cut(s)	21
FriOI	GRGCYC	3 cut(s)	23, 1031, 1182
Fsp4HI	GCNGC	8 cut(s)	9, 420, 594, 698, 822, 1115, 1146, 1217
FspBI	CTAG	2 cut(s)	144, 825
GlaI	GCGC	1 cut(s)	395
GluI	GCNGC	8 cut(s)	9, 420, 594, 698, 822, 1115, 1146, 1217
GsaI	CCCAGC	1 cut(s)	1257
GsuI	CTGGAG	1 cut(s)	240
HaeIII	GGCC	2 cut(s)	1029, 1339
HapII	CCGG	1 cut(s)	376
HgaI	GACGC	1 cut(s)	636
HhaI	GCGC	1 cut(s)	396
Hin1II	CATG	6 cut(s)	157, 661, 677, 725, 845, 1298
Hin6I	GCGC	1 cut(s)	394
HinP1I	GCGC	1 cut(s)	394
HincII	GTYRAC	1 cut(s)	1611
HindII	GTYRAC	1 cut(s)	1611
HindIII	AAGCTT	3 cut(s)	41, 553, 1133
HinfI	GANTC	7 cut(s)	195, 260, 425, 627, 914, 1157, 1498
HpaII	CCGG	1 cut(s)	376
HphI	GGTGA	7 cut(s)	39, 596, 862, 1099, 1152, 1219, 1272
Hpy166II	GTNNAC	4 cut(s)	953, 1280, 1348, 1611
Hpy188I	TCNGA	3 cut(s)	18, 384, 913
Hpy8I	GTNNAC	4 cut(s)	953, 1280, 1348, 1611
HpyAV	CCTTC	5 cut(s)	522, 697, 701, 1088, 1465
HpyCH4III	ACNGT	2 cut(s)	167, 219
HpyCH4IV	ACGT	3 cut(s)	365, 890, 1513
HpyF10VI	GCNNNNNNNGC	5 cut(s)	27, 350, 1000, 1145, 1598
HpyF3I	CTNAG	9 cut(s)	17, 131, 398, 551, 712, 910, 999, 1006, 1649
HpySE526I	ACGT	3 cut(s)	365, 890, 1513
Hsp92II	CATG	6 cut(s)	157, 661, 677, 725, 845, 1298
HspAI	GCGC	1 cut(s)	394
Kzo9I	GATC	3 cut(s)	356, 566, 1656
LguI	GCTCTTC	1 cut(s)	18
LmnI	GCTCC	4 cut(s)	346, 875, 1177, 1291
Lsp1109I	GCAGC	6 cut(s)	406, 605, 684, 1101, 1132, 1228
LweI	GCATC	2 cut(s)	1514, 1562
MaeI	CTAG	2 cut(s)	144, 825
MaeII	ACGT	3 cut(s)	365, 890, 1513
MaeIII	GTNAC	5 cut(s)	296, 463, 850, 1066, 1087
MalI	GATC	3 cut(s)	358, 568, 1658
MbiI	CCGCTC	1 cut(s)	11
MboI	GATC	3 cut(s)	356, 566, 1656
MboII	GAAGA	5 cut(s)	5, 204, 424, 739, 1117
MfeI	CAATTG	2 cut(s)	1479, 1602
MflI	RGATCY	2 cut(s)	356, 566
MhlI	GDGCHC	3 cut(s)	23, 1031, 1182
MlyI	GAGTC	3 cut(s)	204, 269, 1151
MroXI	GAANNNNTTC	1 cut(s)	218
MspI	CCGG	1 cut(s)	376
MspR9I	CCNGG	3 cut(s)	516, 689, 1467
MunI	CAATTG	2 cut(s)	1479, 1602
Mva1269I	GAATGC	3 cut(s)	340, 1218, 1257
MvaI	CCWGG	3 cut(s)	516, 689, 1467
MvnI	CGCG	1 cut(s)	326
MwoI	GCNNNNNNNGC	5 cut(s)	27, 350, 1000, 1145, 1598
NdeII	GATC	3 cut(s)	356, 566, 1656
NheI	GCTAGC	1 cut(s)	824
NlaIII	CATG	6 cut(s)	157, 661, 677, 725, 845, 1298
NlaIV	GGNNCC	3 cut(s)	1028, 1029, 1179
NmuCI	GTSAC	5 cut(s)	296, 463, 850, 1066, 1087
NspI	RCATGY	2 cut(s)	845, 1298
PaeR7I	CTCGAG	1 cut(s)	60
PagI	TCATGA	1 cut(s)	721
PciSI	GCTCTTC	1 cut(s)	18
PctI	GAATGC	3 cut(s)	340, 1218, 1257
PdmI	GAANNNNTTC	1 cut(s)	218
PfeI	GAWTC	4 cut(s)	425, 627, 914, 1498
PinAI	ACCGGT	1 cut(s)	375
PkrI	GCNGC	8 cut(s)	10, 421, 595, 699, 823, 1116, 1147, 1218
PleI	GAGTC	3 cut(s)	203, 268, 1151
PpsI	GAGTC	3 cut(s)	203, 268, 1151
PshAI	GACNNNNGTC	1 cut(s)	1616
Psp124BI	GAGCTC	1 cut(s)	23
Psp6I	CCWGG	3 cut(s)	514, 687, 1465
PspFI	CCCAGC	1 cut(s)	1253
PspGI	CCWGG	3 cut(s)	514, 687, 1465
PspN4I	GGNNCC	3 cut(s)	1028, 1029, 1179
PspOMI	GGGCCC	1 cut(s)	1027
PspPI	GGNCC	3 cut(s)	1027, 1028, 1337
PsrI	GAACNNNNNNTAC	2 cut(s)	1261, 1293
PstI	CTGCAG	1 cut(s)	1119
PstNI	CAGNNNCTG	1 cut(s)	917
PsuI	RGATCY	2 cut(s)	356, 566
RsaI	GTAC	5 cut(s)	666, 954, 1121, 1349, 1487
RsaNI	GTAC	5 cut(s)	665, 953, 1120, 1348, 1486
SacI	GAGCTC	1 cut(s)	23
SapI	GCTCTTC	1 cut(s)	18
SatI	GCNGC	8 cut(s)	9, 420, 594, 698, 822, 1115, 1146, 1217
Sau3AI	GATC	3 cut(s)	356, 566, 1656
Sau96I	GGNCC	3 cut(s)	1027, 1028, 1337
ScaI	AGTACT	1 cut(s)	1121
SchI	GAGTC	3 cut(s)	204, 269, 1151
ScrFI	CCNGG	3 cut(s)	516, 689, 1467
SduI	GDGCHC	3 cut(s)	23, 1031, 1182
SfaNI	GCATC	2 cut(s)	1514, 1562
SfcI	CTRYAG	3 cut(s)	829, 1062, 1115
Sfr274I	CTCGAG	1 cut(s)	60
SlaI	CTCGAG	1 cut(s)	60
SmlI	CTYRAG	2 cut(s)	60, 540
SmoI	CTYRAG	2 cut(s)	60, 540
SsiI	CCGC	7 cut(s)	9, 326, 344, 353, 700, 821, 1592
SspI	AATATT	1 cut(s)	960
SspMI	CTAG	2 cut(s)	144, 825
SstI	GAGCTC	1 cut(s)	23
StyD4I	CCNGG	3 cut(s)	514, 687, 1465
StyI	CCWWGG	2 cut(s)	1340, 1410
TaaI	ACNGT	2 cut(s)	167, 219
TaiI	ACGT	3 cut(s)	368, 893, 1516
TaqI	TCGA	4 cut(s)	61, 212, 1508, 1566
TatI	WGTACW	3 cut(s)	952, 1119, 1347
TauI	GCSGC	2 cut(s)	11, 824
TfiI	GAWTC	4 cut(s)	425, 627, 914, 1498
TscAI	CASTG	2 cut(s)	411, 477
TseFI	GTSAC	5 cut(s)	296, 463, 850, 1066, 1087
TseI	GCWGC	6 cut(s)	419, 593, 697, 1114, 1145, 1216
Tsp45I	GTSAC	5 cut(s)	296, 463, 850, 1066, 1087
TspDTI	ATGAA	4 cut(s)	142, 493, 591, 924
TspGWI	ACGGA	2 cut(s)	273, 320
TspRI	CASTG	2 cut(s)	411, 477
XapI	RAATTY	6 cut(s)	55, 84, 90, 148, 309, 385
XceI	RCATGY	2 cut(s)	845, 1298
XhoI	CTCGAG	1 cut(s)	60
XmnI	GAANNNNTTC	1 cut(s)	218
XspI	CTAG	2 cut(s)	144, 825
ZrmI	AGTACT	1 cut(s)	1121

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.