Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014328

Physical Location & Seq

Genomic Coordinates Reverse (-)

13919127 .. 13925421

Gene Structure 6295 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G32810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

ATGGCAGAGTCAATTCGAACTTTTTCGCTTCAATGGCGGATACTGTCACTGATCATCGCTTTACTTGTGTACTTTCCGATTCTGTCGGGAAGTTACTTTAAACCGTTCAATGTGAGCTACGATCACCGGGCTTTAATCATCGCCGGCAAGAGGCGCATGCTCGTCTCAGCTGGAATTCACTACCCTCGAGCCACTCCTGAGATGTGGTCTGATCTCATCGCAAAGAGTAAAGAAGGTGGAGCTGATGTGGTTCAAACTTATGTGTTTTGGAATGGGCACGAGCCTGTAAAGGGTCAGTATAACTTTGAAGGAAGATATGACCTTGTCAAATTTGTGAAGCTTATTGGATCAAGTGGTCTTTATCTCCATCTACGGATAGGTCCTTATGTTTGTGCTGAGTGGAACTTTGGGGGGTTCCCAGTGTGGCTGCGGGATATTCCTGGCATTGAGTTTCGAACAGATAATGAACCTTTTAAGAAAGAGATGCAAAAGTTTGTAACAAAGATTGTGGATTTGATGCGAGAAGCAAAGCTCTTCTGTTGGCAAGGAGGTCCCATAATCATGCTGCAGATCGAGAATGAATATGGGGATGTTGAAAAATCTTACGGGCAGAAGGGAAAGGATTACGTCAAATGGGCAGCTAGCATGGCTCTAGGGCTTGGTGCTGGAGTTCCGTGGGTTATGTGCAAACAAACGGACGCTCCAGAGAATATTATTGATGCATGCAATGGATACTATTGTGATGGTTTTAAACCAAATTCGCGGACGAAGCCAGTACTTTGGACAGAGGATTGGGATGGATGGTACACGAAGTGGGGTGGAAGTTTGCCTCACCGACCTGCTGAAGATCTTGCATTTGCAGTTGCAAGATTTTATCAGCGTGGAGGAAGCTTTCAAAATTATTATATGTATTTTGGTGGAACAAACTTTGGTCGAACTTCTGGTGGTCCTTTCTACATTACAAGCTATGATTATGATGCTCCTCTTGATGAATATGGTCTGAGGAGCGAGCCAAAATGGGGGCATCTAAAGGACCTTCATGCTGCCATAAAACTTTGTGAACCTGCTCTTGTCGCGGCGGATGCACCTCAATATAGAAAATTGGGATCAAAGCAGGAGGCACACATTTATCATGGAGATGGTGAGACTGGAGGAAAGGTGTGCGCTGCATTTCTCGCAAACATTGATGAGCATAAATCTGCACATGTGAAATTTAATGGCCAGTCATATACTTTACCACCTTGGTCAGTGAGTATATTGCCAGACTGTCGACATGTGGCCTTCAACACTGCCAAGGTAGGAGCACAAACTTCTGTTAAAACGGTTGAGTCTGCAAGGCCATCTTTAGGTAGTATGTCTATACTGCAAAAAGTCGTGAGGCAGGATAATGTTTCTTATATCTCAAAATCATGGATGGCTCTGAAAGAACCTATTGGCATATGGGGTGAAAACAATTTCACTTTCCAAGGATTATTGGAGCATTTGAATGTTACAAAAGACCGGTCTGATTACCTGTGGCATAAGACAAGAATAAGCGTATCTGAAGATGATATCTCGTTCTGGAAGAAAAATGGACCTAACTCAACAGTGTCGATTGATAGCATGAGGGATGTGTTACGTGTGTTTGTTAACAAACAACTCGCAGGCAGTATTGTTGGTCACTGGGTGAAGGCAGTGCAGCCTGTACGATTTATACAGGGAAACAATGATTTACTTCTGTTGACTCAAACAGTTGGTTTACAGAATTATGGTGCTTTCTTGGAGAAGGATGGAGCCGGTTTTAGAGGCAAAGCTAAGCTTACTGGATTCAAGAATGGAGATTTAGACCTGTCAAAATCGTCATGGACGTATCAGGTGGGATTGAAGGGCGAGGCTGATAAAATATATACTGTTGAACACAATGAAAAAGCTGAGTGGAGTACTTTGGAGACCGATGCTTCACCTTCGATCTTCATGTGGTACAAGACATACTTTGACCCCCCGGCCGGAACAGATCCTGTTGTTCTTAATCTGGAAAGTATGGGAAGGGGGCAAGCTTGGGTCAATGGACAACATATTGGAAGGTATTGGAATATCATTTCCCAAAAGGATGGATGTGACAGAACCTGTGATTATCGTGGAGCCTACAATTCAGATAAGTGCACAACAAATTGTGGAAAGCCTACACAAACCAGGTACCATGTACCGCGTTCGTGGTTAAAGCCGTCAAGTAATCTACTTGTGCTCTTTGAAGAGACTGGGGGAAACCCATTTAAGATATCGGTTAAGACAGTTACTGCTGGGATTCTGTGCGGTCAAGTCTCGGAATCGCATTATCCACCTCTGCGGAAATGGTCCACACCAGATTACATAAACGGAACAATGTCGATAAACAGTGTGGCACCAGAAGTGCATCTGCATTGTGAAGATGGACATGTCATATCCTCCATAGAATTTGCTAGCTATGGGACTCCAAGAGGTAGCTGCGACGGGTTTTCTATCGGGAAATGTCATGCATCCAACTCATTATCAATCGTCTCTGAGGCTTGTAAAGGACGCAACAGCTGCTTCATCGAGGTATCAAATACTGCTTTTATCAGTGACCCATGTAGCGGAACTTTAAAGACTTTAGCGGTTATGTCACGATGTTCCCCGTCTCAAAACATGAGTGACCTCAGTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009505 GO:0015925 GO:0016020 GO:0016787 GO:0016798 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

887

Amino Acids

99.2

Weight (kDa)

8.29

Isoelectric Point (pI)

37.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	45 - 349	1.3e-120	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	66 - 211	1.7e-06	Beta-galactosidase
GHD	PF17834	374 - 433	8.5e-23	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	652 - 738	2.9e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	799 - 876	1.1e-21	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32810 AT2G32810
fragaria_vesca	FvH4_4g13490
malus_domestica	MD13G1283100.v1.1
prunus_persica	Prupe.1G160600_v2.0.a1 Prupe.1G160600_v2.0.a1
pyrus_communis	pycom420g01190
rosa_chinensis	RchiOBHm_Chr4g0414111
rosa_laevigata	RLG00000008175
rosa_multiflora	Rmu_sc0003264.1_g000008
rosa_roxburghii	Rroxscaffold_5G00358040
rosa_rugosa	Rorug04G0125600 Rorug04G0125700
rosa_samantha	Rh4AG184200 Rh4BG183700 Rh4DG179600
rosa_wichuraiana	Rw4G015680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	847, 1072
Acc65I	GGTACC	1 cut(s)	2175
AccB1I	GGYRCC	2 cut(s)	2175, 2380
AccI	GTMKAC	1 cut(s)	1270
AccII	CGCG	3 cut(s)	763, 1076, 2188
AclWI	GGATC	3 cut(s)	355, 1114, 1988
AcoI	YGGCCR	2 cut(s)	1219, 1983
AcsI	RAATTY	5 cut(s)	174, 329, 757, 1211, 2432
AcuI	CTGAAG	2 cut(s)	864, 1563
AdeI	CACNNNGTG	2 cut(s)	813, 1666
AfaI	GTAC	8 cut(s)	71, 777, 806, 1686, 1921, 1961, 2177, 2184
AfiI	CCNNNNNNNGG	6 cut(s)	150, 290, 1019, 1346, 1985, 2591
AflIII	ACRYGT	4 cut(s)	1204, 1273, 1618, 2413
AgeI	ACCGGT	1 cut(s)	1500
AjnI	CCWGG	2 cut(s)	439, 2171
Alw21I	GWGCWC	3 cut(s)	1306, 2144, 2226
Alw26I	GTCTC	7 cut(s)	169, 1139, 1922, 2228, 2305, 2521, 2640
Alw44I	GTGCAC	1 cut(s)	2140
AlwI	GGATC	3 cut(s)	355, 1114, 1988
AlwNI	CAGNNNCTG	3 cut(s)	1997, 2106, 2276
Ama87I	CYCGRG	1 cut(s)	186
AoxI	GGCC	4 cut(s)	1219, 1278, 1337, 1983
ApaLI	GTGCAC	1 cut(s)	2140
ApeKI	GCWGC	8 cut(s)	427, 565, 638, 1043, 1166, 1678, 2463, 2544
ApoI	RAATTY	5 cut(s)	174, 329, 757, 1211, 2432
ArsI	GACNNNNNNTTYG	2 cut(s)	1487, 1519
AsiGI	ACCGGT	1 cut(s)	1500
Asp700I	GAANNNNTTC	1 cut(s)	22
Asp718I	GGTACC	1 cut(s)	2175
AspLEI	GCGC	2 cut(s)	156, 1166
AspS9I	GGNCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
AsuC2I	CCSGG	2 cut(s)	128, 1982
AsuHPI	GGTGA	6 cut(s)	116, 824, 1154, 1457, 1678, 1932
AsuII	TTCGAA	2 cut(s)	16, 454
AsuNHI	GCTAGC	2 cut(s)	641, 2438
AvaI	CYCGRG	1 cut(s)	186
AvaII	GGWCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
BaeGI	GKGCMC	2 cut(s)	279, 2144
BaeI	ACNNNNGTAYC	2 cut(s)	724, 757
BalI	TGGCCA	1 cut(s)	1221
BanI	GGYRCC	2 cut(s)	2175, 2380
BauI	CACGAG	1 cut(s)	278
Bbv12I	GWGCWC	3 cut(s)	1306, 2144, 2226
BbvI	GCAGC	8 cut(s)	414, 552, 650, 1030, 1153, 1690, 2450, 2531
BceAI	ACGGC	1 cut(s)	2188
BciT130I	CCWGG	2 cut(s)	441, 2173
BciVI	GTATCC	2 cut(s)	33, 725
BclI	TGATCA	1 cut(s)	51
BcnI	CCSGG	2 cut(s)	128, 1982
BcoDI	GTCTC	7 cut(s)	169, 1139, 1922, 2228, 2305, 2521, 2640
BfaI	CTAG	3 cut(s)	642, 653, 2439
BfmI	CTRYAG	1 cut(s)	566
BfuAI	ACCTGC	2 cut(s)	847, 1072
BfuI	GTATCC	2 cut(s)	33, 725
BglII	AGATCT	1 cut(s)	847
BisI	GCNGC	9 cut(s)	428, 566, 639, 1044, 1077, 1167, 1679, 2464, 2545
BlpI	GCTNAGC	1 cut(s)	1794
BlsI	GCNGC	9 cut(s)	429, 567, 640, 1045, 1078, 1168, 1680, 2465, 2546
BmcAI	AGTACT	2 cut(s)	777, 1921
Bme1390I	CCNGG	4 cut(s)	128, 441, 1982, 2173
Bme18I	GGWCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
BmeT110I	CYCGRG	1 cut(s)	186
BmgT120I	GGNCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
BmiI	GGNNCC	6 cut(s)	416, 553, 1774, 2122, 2177, 2382
BmrFI	CCNGG	4 cut(s)	128, 441, 1982, 2173
BmrI	ACTGGG	3 cut(s)	413, 1672, 2247
BmsI	GCATC	9 cut(s)	474, 507, 709, 967, 1033, 1072, 1924, 2401, 2504
BmtI	GCTAGC	2 cut(s)	645, 2442
BmuI	ACTGGG	3 cut(s)	413, 1672, 2247
BpmI	CTGGAG	3 cut(s)	687, 687, 1170
Bpu1102I	GCTNAGC	1 cut(s)	1794
Bpu14I	TTCGAA	2 cut(s)	16, 454
BpuMI	CCSGG	2 cut(s)	128, 1982
BsaAI	YACGTR	1 cut(s)	1619
BsaI	GGTCTC	1 cut(s)	1922
BsaJI	CCNNGG	5 cut(s)	674, 1241, 1293, 1465, 1980
BsaWI	WCCGGW	1 cut(s)	1500
BsaXI	ACNNNNNCTCC	6 cut(s)	231, 261, 685, 715, 1143, 1173
Bsc4I	CCNNNNNNNGG	6 cut(s)	150, 290, 1019, 1346, 1985, 2591
Bse118I	RCCGGY	3 cut(s)	143, 1500, 1775
Bse1I	ACTGG	7 cut(s)	419, 773, 1153, 1222, 1667, 1807, 2242
Bse3DI	GCAATG	1 cut(s)	733
BseBI	CCWGG	2 cut(s)	441, 2173
BseDI	CCNNGG	5 cut(s)	674, 1241, 1293, 1465, 1980
BseLI	CCNNNNNNNGG	6 cut(s)	150, 290, 1019, 1346, 1985, 2591
BseMI	GCAATG	1 cut(s)	733
BseMII	CTCAG	6 cut(s)	180, 189, 387, 992, 1902, 2511
BseNI	ACTGG	7 cut(s)	419, 773, 1153, 1222, 1667, 1807, 2242
BseRI	GAGGAG	2 cut(s)	972, 1018
BseSI	GKGCMC	2 cut(s)	279, 2144
BseX3I	CGGCCG	1 cut(s)	1983
BseXI	GCAGC	8 cut(s)	414, 552, 650, 1030, 1153, 1690, 2450, 2531
BseYI	CCCAGC	1 cut(s)	2279
BsgI	GTGCAG	2 cut(s)	1185, 1697
Bsh1236I	CGCG	3 cut(s)	763, 1076, 2188
Bsh1285I	CGRYCG	1 cut(s)	1986
BshFI	GGCC	4 cut(s)	1221, 1280, 1339, 1985
BshNI	GGYRCC	2 cut(s)	2175, 2380
BshTI	ACCGGT	1 cut(s)	1500
BsiEI	CGRYCG	1 cut(s)	1986
BsiHKAI	GWGCWC	3 cut(s)	1306, 2144, 2226
BsiHKCI	CYCGRG	1 cut(s)	186
BsiSI	CCGG	6 cut(s)	127, 144, 1501, 1776, 1982, 1986
BslFI	GGGAC	2 cut(s)	537, 2461
BslI	CCNNNNNNNGG	6 cut(s)	150, 290, 1019, 1346, 1985, 2591
BsmAI	GTCTC	7 cut(s)	169, 1139, 1922, 2228, 2305, 2521, 2640
BsmBI	CGTCTC	3 cut(s)	169, 2521, 2640
BsmFI	GGGAC	2 cut(s)	537, 2461
BsnI	GGCC	4 cut(s)	1221, 1280, 1339, 1985
Bso31I	GGTCTC	1 cut(s)	1922
BsoBI	CYCGRG	1 cut(s)	186
Bsp119I	TTCGAA	2 cut(s)	16, 454
Bsp1286I	GDGCHC	4 cut(s)	279, 1306, 2144, 2226
Bsp143I	GATC	9 cut(s)	51, 121, 211, 347, 570, 847, 1106, 1947, 1993
Bsp1720I	GCTNAGC	1 cut(s)	1794
BspANI	GGCC	4 cut(s)	1221, 1280, 1339, 1985
BspCNI	CTCAG	6 cut(s)	179, 190, 388, 993, 1903, 2512
BspFNI	CGCG	3 cut(s)	763, 1076, 2188
BspLI	GGNNCC	6 cut(s)	416, 553, 1774, 2122, 2177, 2382
BspMAI	CTGCAG	1 cut(s)	570
BspMI	ACCTGC	2 cut(s)	847, 1072
BspOI	GCTAGC	2 cut(s)	645, 2442
BspPI	GGATC	3 cut(s)	355, 1114, 1988
BspQI	GCTCTTC	1 cut(s)	539
BspT104I	TTCGAA	2 cut(s)	16, 454
BspT107I	GGYRCC	2 cut(s)	2175, 2380
BspTNI	GGTCTC	1 cut(s)	1922
BsrDI	GCAATG	1 cut(s)	733
BsrFI	RCCGGY	3 cut(s)	143, 1500, 1775
BsrI	ACTGG	7 cut(s)	419, 773, 1153, 1222, 1667, 1807, 2242
BssAI	RCCGGY	3 cut(s)	143, 1500, 1775
BssECI	CCNNGG	5 cut(s)	674, 1241, 1293, 1465, 1980
BssMI	GATC	9 cut(s)	51, 121, 211, 347, 570, 847, 1106, 1947, 1993
BssSI	CACGAG	1 cut(s)	278
BssT1I	CCWWGG	3 cut(s)	1241, 1293, 1465
Bst2BI	CACGAG	1 cut(s)	278
Bst2UI	CCWGG	2 cut(s)	441, 2173
Bst4CI	ACNGT	9 cut(s)	45, 105, 1268, 1324, 1588, 1732, 1891, 2272, 2375
Bst6I	CTCTTC	2 cut(s)	539, 2226
BstAPI	GCANNNNNTGC	1 cut(s)	2544
BstBAI	YACGTR	1 cut(s)	1619
BstBI	TTCGAA	2 cut(s)	16, 454
BstC8I	GCNNGC	8 cut(s)	145, 158, 643, 724, 1010, 1645, 2034, 2440
BstDEI	CTNAG	8 cut(s)	166, 198, 396, 1001, 1794, 1911, 2520, 2654
BstDSI	CCRYGG	1 cut(s)	674
BstFNI	CGCG	3 cut(s)	763, 1076, 2188
BstHHI	GCGC	2 cut(s)	156, 1166
BstKTI	GATC	9 cut(s)	54, 124, 214, 350, 573, 850, 1109, 1950, 1996
BstMAI	GTCTC	7 cut(s)	169, 1139, 1922, 2228, 2305, 2521, 2640
BstMBI	GATC	9 cut(s)	51, 121, 211, 347, 570, 847, 1106, 1947, 1993
BstMCI	CGRYCG	1 cut(s)	1986
BstMWI	GCNNNNNNNGC	8 cut(s)	34, 153, 647, 769, 1073, 1082, 1175, 2544
BstNI	CCWGG	2 cut(s)	441, 2173
BstNSI	RCATGY	5 cut(s)	160, 726, 1208, 1277, 2417
BstSCI	CCNGG	4 cut(s)	126, 439, 1980, 2171
BstSFI	CTRYAG	1 cut(s)	566
BstSLI	GKGCMC	2 cut(s)	279, 2144
BstUI	CGCG	3 cut(s)	763, 1076, 2188
BstV1I	GCAGC	8 cut(s)	414, 552, 650, 1030, 1153, 1690, 2450, 2531
BstX2I	RGATCY	2 cut(s)	847, 1993
BstXI	CCANNNNNNTGG	2 cut(s)	780, 2090
BstYI	RGATCY	2 cut(s)	847, 1993
BstZI	CGGCCG	1 cut(s)	1983
BsuI	GTATCC	2 cut(s)	33, 725
BsuRI	GGCC	4 cut(s)	1221, 1280, 1339, 1985
BtgI	CCRYGG	1 cut(s)	674
BtgZI	GCGATG	3 cut(s)	40, 124, 202
BtsI	GCAGTG	2 cut(s)	1287, 1680
BtsIMutI	CAGTG	9 cut(s)	47, 426, 1254, 1287, 1593, 1660, 1680, 2380, 2584
BveI	ACCTGC	2 cut(s)	847, 1072
Cac8I	GCNNGC	8 cut(s)	145, 158, 643, 724, 1010, 1645, 2034, 2440
CaiI	CAGNNNCTG	3 cut(s)	1997, 2106, 2276
CfoI	GCGC	2 cut(s)	156, 1166
Cfr10I	RCCGGY	3 cut(s)	143, 1500, 1775
Cfr13I	GGNCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
CseI	GACGC	2 cut(s)	707, 2544
CsiI	ACCWGGT	1 cut(s)	2171
Csp6I	GTAC	8 cut(s)	70, 776, 805, 1685, 1920, 1960, 2176, 2183
CspAI	ACCGGT	1 cut(s)	1500
CspCI	CAANNNNNGTGG	2 cut(s)	489, 524
CviQI	GTAC	8 cut(s)	70, 776, 805, 1685, 1920, 1960, 2176, 2183
DdeI	CTNAG	8 cut(s)	166, 198, 396, 1001, 1794, 1911, 2520, 2654
DpnI	GATC	9 cut(s)	53, 123, 213, 349, 572, 849, 1108, 1949, 1995
DpnII	GATC	9 cut(s)	51, 121, 211, 347, 570, 847, 1106, 1947, 1993
DraI	TTTAAA	3 cut(s)	100, 751, 2601
DraIII	CACNNNGTG	2 cut(s)	813, 1666
EaeI	YGGCCR	2 cut(s)	1219, 1983
EagI	CGGCCG	1 cut(s)	1983
Eam1104I	CTCTTC	2 cut(s)	539, 2226
EarI	CTCTTC	2 cut(s)	539, 2226
EciI	GGCGGA	2 cut(s)	52, 1094
EclXI	CGGCCG	1 cut(s)	1983
Eco130I	CCWWGG	3 cut(s)	1241, 1293, 1465
Eco31I	GGTCTC	1 cut(s)	1922
Eco32I	GATATC	2 cut(s)	1552, 2259
Eco47I	GGWCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
Eco52I	CGGCCG	1 cut(s)	1983
Eco57I	CTGAAG	2 cut(s)	864, 1563
Eco88I	CYCGRG	1 cut(s)	186
EcoO109I	RGGNCCY	3 cut(s)	380, 551, 1033
EcoRI	GAATTC	1 cut(s)	174
EcoRII	CCWGG	2 cut(s)	439, 2171
EcoRV	GATATC	2 cut(s)	1552, 2259
EcoT14I	CCWWGG	3 cut(s)	1241, 1293, 1465
EcoT22I	ATGCAT	2 cut(s)	724, 2497
ErhI	CCWWGG	3 cut(s)	1241, 1293, 1465
Esp3I	CGTCTC	3 cut(s)	169, 2521, 2640
FalI	AAGNNNNNCTT	6 cut(s)	1327, 1359, 1782, 1814, 1955, 1987
FaqI	GGGAC	2 cut(s)	537, 2461
FauI	CCCGC	1 cut(s)	423
FauNDI	CATATG	1 cut(s)	1439
FbaI	TGATCA	1 cut(s)	51
FblI	GTMKAC	1 cut(s)	1270
Fnu4HI	GCNGC	9 cut(s)	428, 566, 639, 1044, 1077, 1167, 1679, 2464, 2545
Fsp4HI	GCNGC	9 cut(s)	428, 566, 639, 1044, 1077, 1167, 1679, 2464, 2545
FspBI	CTAG	3 cut(s)	642, 653, 2439
GlaI	GCGC	2 cut(s)	155, 1165
GluI	GCNGC	9 cut(s)	428, 566, 639, 1044, 1077, 1167, 1679, 2464, 2545
GsaI	CCCAGC	1 cut(s)	2283
GsuI	CTGGAG	3 cut(s)	687, 687, 1170
HaeIII	GGCC	4 cut(s)	1221, 1280, 1339, 1985
HapII	CCGG	6 cut(s)	127, 144, 1501, 1776, 1982, 1986
HgaI	GACGC	2 cut(s)	707, 2544
HhaI	GCGC	2 cut(s)	156, 1166
Hin6I	GCGC	2 cut(s)	154, 1164
HinP1I	GCGC	2 cut(s)	154, 1164
HincII	GTYRAC	3 cut(s)	1271, 1630, 1722
HindII	GTYRAC	3 cut(s)	1271, 1630, 1722
HindIII	AAGCTT	4 cut(s)	338, 889, 1796, 2034
HinfI	GANTC	8 cut(s)	8, 79, 1328, 1723, 1806, 2284, 2306, 2449
HpaI	GTTAAC	1 cut(s)	1630
HpaII	CCGG	6 cut(s)	127, 144, 1501, 1776, 1982, 1986
HphI	GGTGA	6 cut(s)	116, 824, 1154, 1457, 1678, 1932
Hpy166II	GTNNAC	9 cut(s)	70, 807, 1061, 1271, 1630, 1722, 1739, 2142, 2337
Hpy188I	TCNGA	9 cut(s)	78, 211, 1002, 1422, 1507, 1543, 2134, 2305, 2521
Hpy8I	GTNNAC	9 cut(s)	70, 807, 1061, 1271, 1630, 1722, 1739, 2142, 2337
Hpy99I	CGWCG	1 cut(s)	2471
HpyCH4III	ACNGT	9 cut(s)	45, 105, 1268, 1324, 1588, 1732, 1891, 2272, 2375
HpyCH4IV	ACGT	3 cut(s)	627, 1618, 1847
HpyF10VI	GCNNNNNNNGC	8 cut(s)	34, 153, 647, 769, 1073, 1082, 1175, 2544
HpyF3I	CTNAG	8 cut(s)	166, 198, 396, 1001, 1794, 1911, 2520, 2654
HpySE526I	ACGT	3 cut(s)	627, 1618, 1847
HspAI	GCGC	2 cut(s)	154, 1164
KpnI	GGTACC	1 cut(s)	2179
KroI	GCCGGC	1 cut(s)	143
KroNI	GCCGGC	1 cut(s)	145
Ksp22I	TGATCA	1 cut(s)	51
KspAI	GTTAAC	1 cut(s)	1630
Kzo9I	GATC	9 cut(s)	51, 121, 211, 347, 570, 847, 1106, 1947, 1993
LguI	GCTCTTC	1 cut(s)	539
LmnI	GCTCC	8 cut(s)	239, 706, 985, 1005, 1301, 1477, 1772, 2120
Lsp1109I	GCAGC	8 cut(s)	414, 552, 650, 1030, 1153, 1690, 2450, 2531
LweI	GCATC	9 cut(s)	474, 507, 709, 967, 1033, 1072, 1924, 2401, 2504
MabI	ACCWGGT	1 cut(s)	2171
MaeI	CTAG	3 cut(s)	642, 653, 2439
MaeII	ACGT	3 cut(s)	627, 1618, 1847
MalI	GATC	9 cut(s)	53, 123, 213, 349, 572, 849, 1108, 1949, 1995
MboI	GATC	9 cut(s)	51, 121, 211, 347, 570, 847, 1106, 1947, 1993
MboII	GAAGA	8 cut(s)	324, 526, 857, 1556, 1576, 1942, 2243, 2417
MflI	RGATCY	2 cut(s)	847, 1993
MhlI	GDGCHC	4 cut(s)	279, 1306, 2144, 2226
MlsI	TGGCCA	1 cut(s)	1221
MluNI	TGGCCA	1 cut(s)	1221
MlyI	GAGTC	4 cut(s)	17, 1337, 1717, 2443
MmeI	TCCRAC	1 cut(s)	2523
Mox20I	TGGCCA	1 cut(s)	1221
Mph1103I	ATGCAT	2 cut(s)	724, 2497
MroNI	GCCGGC	1 cut(s)	143
MroXI	GAANNNNTTC	1 cut(s)	22
MscI	TGGCCA	1 cut(s)	1221
MslI	CAYNNNNRTG	3 cut(s)	560, 1137, 1485
Msp20I	TGGCCA	1 cut(s)	1221
MspA1I	CMGCKG	2 cut(s)	170, 2544
MspI	CCGG	6 cut(s)	127, 144, 1501, 1776, 1982, 1986
MspR9I	CCNGG	4 cut(s)	128, 441, 1982, 2173
MvaI	CCWGG	2 cut(s)	441, 2173
MvnI	CGCG	3 cut(s)	763, 1076, 2188
MwoI	GCNNNNNNNGC	8 cut(s)	34, 153, 647, 769, 1073, 1082, 1175, 2544
NaeI	GCCGGC	1 cut(s)	145
NciI	CCSGG	2 cut(s)	128, 1982
NdeI	CATATG	1 cut(s)	1439
NdeII	GATC	9 cut(s)	51, 121, 211, 347, 570, 847, 1106, 1947, 1993
NgoMIV	GCCGGC	1 cut(s)	143
NheI	GCTAGC	2 cut(s)	641, 2438
NlaIV	GGNNCC	6 cut(s)	416, 553, 1774, 2122, 2177, 2382
NmuCI	GTSAC	6 cut(s)	45, 1658, 2096, 2579, 2619, 2648
NsiI	ATGCAT	2 cut(s)	724, 2497
NspI	RCATGY	5 cut(s)	160, 726, 1208, 1277, 2417
NspV	TTCGAA	2 cut(s)	16, 454
PaeI	GCATGC	2 cut(s)	160, 726
PaeR7I	CTCGAG	1 cut(s)	186
PciI	ACATGT	3 cut(s)	1204, 1273, 2413
PciSI	GCTCTTC	1 cut(s)	539
PcsI	WCGNNNNNNNCGW	1 cut(s)	1844
PdiI	GCCGGC	1 cut(s)	145
PdmI	GAANNNNTTC	1 cut(s)	22
PfeI	GAWTC	4 cut(s)	79, 1806, 2284, 2306
PflFI	GACNNNGTC	1 cut(s)	323
PinAI	ACCGGT	1 cut(s)	1500
PkrI	GCNGC	9 cut(s)	429, 567, 640, 1045, 1078, 1168, 1680, 2465, 2546
PleI	GAGTC	4 cut(s)	16, 1336, 1717, 2443
PpsI	GAGTC	4 cut(s)	16, 1336, 1717, 2443
Ppu21I	YACGTR	1 cut(s)	1619
PpuMI	RGGWCCY	3 cut(s)	380, 551, 1033
PscI	ACATGT	3 cut(s)	1204, 1273, 2413
Psp5II	RGGWCCY	3 cut(s)	380, 551, 1033
Psp6I	CCWGG	2 cut(s)	439, 2171
PspFI	CCCAGC	1 cut(s)	2279
PspGI	CCWGG	2 cut(s)	439, 2171
PspN4I	GGNNCC	6 cut(s)	416, 553, 1774, 2122, 2177, 2382
PspPI	GGNCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
PspPPI	RGGWCCY	3 cut(s)	380, 551, 1033
PspXI	VCTCGAGB	1 cut(s)	186
PstI	CTGCAG	1 cut(s)	570
PstNI	CAGNNNCTG	3 cut(s)	1997, 2106, 2276
PsuI	RGATCY	2 cut(s)	847, 1993
PsyI	GACNNNGTC	1 cut(s)	323
PvuII	CAGCTG	2 cut(s)	170, 2544
RsaI	GTAC	8 cut(s)	71, 777, 806, 1686, 1921, 1961, 2177, 2184
RsaNI	GTAC	8 cut(s)	70, 776, 805, 1685, 1920, 1960, 2176, 2183
RseI	CAYNNNNRTG	3 cut(s)	560, 1137, 1485
SalI	GTCGAC	1 cut(s)	1269
SapI	GCTCTTC	1 cut(s)	539
SatI	GCNGC	9 cut(s)	428, 566, 639, 1044, 1077, 1167, 1679, 2464, 2545
Sau3AI	GATC	9 cut(s)	51, 121, 211, 347, 570, 847, 1106, 1947, 1993
Sau96I	GGNCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
ScaI	AGTACT	2 cut(s)	777, 1921
SchI	GAGTC	4 cut(s)	17, 1337, 1717, 2443
ScrFI	CCNGG	4 cut(s)	128, 441, 1982, 2173
SduI	GDGCHC	4 cut(s)	279, 1306, 2144, 2226
SexAI	ACCWGGT	1 cut(s)	2171
SfaNI	GCATC	9 cut(s)	474, 507, 709, 967, 1033, 1072, 1924, 2401, 2504
SfcI	CTRYAG	1 cut(s)	566
Sfr274I	CTCGAG	1 cut(s)	186
SfuI	TTCGAA	2 cut(s)	16, 454
SinI	GGWCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
SlaI	CTCGAG	1 cut(s)	186
SmiMI	CAYNNNNRTG	3 cut(s)	560, 1137, 1485
SmlI	CTYRAG	1 cut(s)	186
SmoI	CTYRAG	1 cut(s)	186
SphI	GCATGC	2 cut(s)	160, 726
SspI	AATATT	1 cut(s)	712
SspMI	CTAG	3 cut(s)	642, 653, 2439
StyD4I	CCNGG	4 cut(s)	126, 439, 1980, 2171
StyI	CCWWGG	3 cut(s)	1241, 1293, 1465
TaaI	ACNGT	9 cut(s)	45, 105, 1268, 1324, 1588, 1732, 1891, 2272, 2375
TaiI	ACGT	3 cut(s)	630, 1621, 1850
TaqII	GACCGA	1 cut(s)	1946
TatI	WGTACW	3 cut(s)	69, 775, 1919
TauI	GCSGC	1 cut(s)	1079
TfiI	GAWTC	4 cut(s)	79, 1806, 2284, 2306
TscAI	CASTG	9 cut(s)	54, 426, 1254, 1294, 1593, 1667, 1680, 2380, 2584
TseFI	GTSAC	6 cut(s)	45, 1658, 2096, 2579, 2619, 2648
TseI	GCWGC	8 cut(s)	427, 565, 638, 1043, 1166, 1678, 2463, 2544
Tsp45I	GTSAC	6 cut(s)	45, 1658, 2096, 2579, 2619, 2648
TspDTI	ATGAA	7 cut(s)	480, 594, 1005, 1028, 1917, 1942, 2539
TspGWI	ACGGA	4 cut(s)	388, 663, 710, 2370
TspRI	CASTG	9 cut(s)	54, 426, 1254, 1294, 1593, 1667, 1680, 2380, 2584
Tth111I	GACNNNGTC	1 cut(s)	323
VneI	GTGCAC	1 cut(s)	2140
VpaK11BI	GGWCC	6 cut(s)	380, 551, 947, 1033, 1574, 2334
XapI	RAATTY	5 cut(s)	174, 329, 757, 1211, 2432
XceI	RCATGY	5 cut(s)	160, 726, 1208, 1277, 2417
XhoI	CTCGAG	1 cut(s)	186
XmiI	GTMKAC	1 cut(s)	1270
XmnI	GAANNNNTTC	1 cut(s)	22
XspI	CTAG	3 cut(s)	642, 653, 2439
ZrmI	AGTACT	2 cut(s)	777, 1921
Zsp2I	ATGCAT	2 cut(s)	724, 2497

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.